RESUMEN
Three experimental assays with Duddingtonia flagrans (isolated AC001) were carried out. The growth of the genus Duddingtonia present in formulation of rice bran, its predatory capability on Oesophagostomum spp. infective larvae (L3) in petri dishes (assay 1), its action in faecal cultures with eggs of that parasite (assay 2) and isolate's capability of predation after passing through gastrointestinal tract of swine (assay 3) was evaluated. At assay 3, feces were collected at time intervals of 12, 24, 36, 48, and 60 h after feed animals with the formulation. Assays 1 and 2 showed a statistical difference (p < 0.01) by the F test when comparing the treated group with the control group. At the both assays, was observed in the treated group a reduction percentage of 74.18% and 88.38%, respectively. In assay 3, there was a statistical difference between the treated group and the control group at all collection times (p < 0.01). Regarding the collection periods, there was no statistical difference over time in the treatment group (p > 0.05). The results demonstrate that the fungal isolate AC001 formulated in rice bran can prey on L3 of Oesophagostomum spp., in vitro and after passing through the gastrointestinal tract, without loss of viability. This isolate may be an alternative in the control of Oesophagostomum spp. in swine.
Asunto(s)
Duddingtonia/fisiología , Parasitosis Intestinales/veterinaria , Esofagostomiasis/veterinaria , Control Biológico de Vectores/métodos , Enfermedades de los Porcinos/prevención & control , Animales , Duddingtonia/crecimiento & desarrollo , Heces/parasitología , Femenino , Parasitosis Intestinales/parasitología , Parasitosis Intestinales/prevención & control , Masculino , Esofagostomiasis/prevención & control , Oesophagostomum/microbiología , Oryza/microbiología , Recuento de Huevos de Parásitos/veterinaria , Esporas Fúngicas/crecimiento & desarrollo , Porcinos , Enfermedades de los Porcinos/parasitologíaRESUMEN
One isolate of predator fungi Duddingtonia flagrans (AC001) was assessed in vitro regarding the capacity of supporting the passage through pigs' gastrointestinal tract without loss of the ability of preying infective larvae Oesophagostomum spp. Fungal isolates survived the passage and were efficient in preying L(3) since the first 8 h of collection (p < 0.01) in relation to the control group (without fungus). Compared with control, there was a significant decrease (p < 0.01) of 59.6% (8 h), 71.7% (12 h), 76.8% (24 h), 81.0% (36 h), 78.0% (48 h), 76.1% (72 h), and 82.7% (96 h) in means of infective larvae Oesophagostomum spp. recovered from treatments with isolate AC001. Linear regression coefficients of L(3) of recovered Oesophagostomum spp. regarding the collections due to time were -0.621 for control, -1.40 for AC001, and -2.64 for NF34. Fungi D. flagrans (AC001) had demonstrated to be promising for use in the biological control of pig parasite Oesophagostomum spp.