RESUMEN
FALC cells are natural helper cells producing Th2-type cytokines, which express c-kit, Sca-1, IL7R and CD45 in mouse and human. These cells are involved in allergic responses and contribute to the inflammatory reactions of adipose tissue; however, a lack of information prevails about the presence of these cells in other species. The aim of the study was to identify and characterise FALC cells in the common carp (Cyprinus carpio) using immunohistochemistry and molecular biology techniques as well as to explore their relationships with their microenvironment. Histological description of the FALC was performed using H&E and polyclonal antibodies were used against cell-surface markers such as c-kit, Sca-1 and CD45. Furthermore, gene expression of c-kit, Sca-1 and IL7R was assessed. C. carpio FALC cells express the same surface markers reported in FALC of the mouse at both the pre- and post-transcriptional level. By exposure to the soluble fraction of helminths, FALC cells produce abundant Th2 cytokines (IL-5, IL-6 and IL-13) but do not synthesise IL-1α. Additionally, FALC cells probably participate in vascular remodelling of the intestine vessels, inducing tumours because a malignant haemangiosarcoma in the peritoneal cavity was found. In this tumour, abundant FALC with their characteristic cell-surface markers were detected. The findings of this study suggest the involvement of some proto-oncogenes such as c-kit and Sca-1, and the deregulation of Src kinases modulated by CD45 present in C. carpio FALC with the ontogeny of peritoneal haemangiosarcoma in this fish species.
Asunto(s)
Carpas/inmunología , Enfermedades de los Peces/inmunología , Hemangiosarcoma/veterinaria , Grasa Intraabdominal/inmunología , Tejido Linfoide/inmunología , Neoplasias Peritoneales/veterinaria , Análisis de Varianza , Animales , Secuencia de Bases , Análisis por Conglomerados , Citocinas/biosíntesis , Cartilla de ADN/genética , ADN Complementario/genética , Ensayo de Inmunoadsorción Enzimática , Hemangiosarcoma/inmunología , Inmunohistoquímica/veterinaria , Antígenos Comunes de Leucocito/genética , Antígenos Comunes de Leucocito/metabolismo , Funciones de Verosimilitud , Tejido Linfoide/citología , Tejido Linfoide/metabolismo , Modelos Genéticos , Datos de Secuencia Molecular , Neoplasias Peritoneales/inmunología , Filogenia , Proteínas Proto-Oncogénicas c-kit/genética , Proteínas Proto-Oncogénicas c-kit/metabolismo , Receptores de Interleucina-7/genética , Receptores de Interleucina-7/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADNRESUMEN
The authors report one case of peritoneal splenosis. The patient submitted to a gastrectomy had a past history of traumatic rupture of the spleen. The morphologic and immunologic aspects of splenosis are discussed. The value of imaging splenic tissue by scynthscanning are pointed out in basis of infection prophylaxis.