RESUMEN
There is opinion in the literature as to that liver trematode infections, such as opisthorchiasis, clonorchiasis, fascioliasis, and metorchiasis, can induce cancer of the liver pancreas, intestine - this all is clinically observed. The authors were the first in world practice to show the development of a hepatic blastomatous process in animals (albino rats, cats) with opisthorchiasis in 13%; cancer developed in 28 and 56% with the use of a hepatotropic carcinogen and combined (opisthorchiasis + a carcinogen) exposure, respectively. Throughout his life, a human being can easily catch these trematodes that have carcinogenic activity and these diseases concurrent with household and food carcinogens can give rise to tumors in the liver pancreas and intestine. Timely diagnosis and specific anthelmintic therapy are necessary to prevent parasitic cancer.
Asunto(s)
Cocarcinogénesis , Neoplasias Hepáticas Experimentales/parasitología , Hígado/parasitología , Opistorquiasis/complicaciones , Alimentación Animal , Animales , Pruebas de Carcinogenicidad , Gatos , Peces/parasitología , Hígado/efectos de los fármacos , Hígado/patología , Neoplasias Hepáticas Experimentales/inducido químicamente , Neoplasias Hepáticas Experimentales/etiología , Neoplasias Hepáticas Experimentales/patología , Opistorquiasis/parasitología , Opisthorchis/aislamiento & purificación , Opisthorchis/patogenicidad , Ratas , p-Dimetilaminoazobenceno/toxicidadRESUMEN
Trematoda O. felinius-induced hepatic lesions were investigated in Syrian golden hamsters with superinvasive opistorchiasis. One hundred hamsters were divided into 4 groups: (1)--control; (2) N-nitrosodiethylamine (DENA), i.p., twice a week, 3 weeks, total dose 72 mg/kg; (3) metacercariae O. felinius, with drinking water, 3 injections per day, once in 2 weeks, and (4) metacercariae O. felinius, as in group 3, followed by DENA, as in group 2. Animals were sacrificed 12 months after the beginning of the study. No changes in the liver were found in group 2. Reddish protrusions, up to 4 cm in diameter, appeared on liver surfaces in groups 3 and 4. Group 4 featured the highest relative and absolute weights of liver as well as clusters of oval cells and cholangiocellular tubules and cholangiofibrosis (in group 3, they were less visible). Electron microscopic examination identified hepatocytes with destructive changes to plasmalemma, nucleus and cytoplasmic organelles. Also, perisinusoidal cells (Ito cells) occurred. Tumor-bearing animals showed low hepatic cytochrome P-450. It is suggested that proliferative growth in the liver was stimulated by opistorchis invasion.
Asunto(s)
Neoplasias Hepáticas Experimentales/parasitología , Hígado/patología , Hígado/parasitología , Opistorquiasis/complicaciones , Animales , Carcinógenos , Cricetinae , Dietilnitrosamina , Fibrosis , Hígado/ultraestructura , Neoplasias Hepáticas Experimentales/inducido químicamente , Neoplasias Hepáticas Experimentales/patología , Mesocricetus , Microscopía Electrónica , Opistorquiasis/patología , Tamaño de los ÓrganosRESUMEN
Utilizing the experimental model in Syrian golden hamsters, we explored the role of immunization in carcinogenesis. The animals, which were infected with liver flukes (Opisthorchis viverrini), and administered a subcarcinogenic dose of dimethylnitrosamine, developed cancer. Pre-immunizing with a crude somatic antigen did not reduce cancer development, but accelerated carcinogenesis. Histopathological analysis of the cancer tissues was done once at week 30 and again at week 39 using H and E staining, immunostaining for the p53 tumor suppressor phosphoprotein, and electron microscopy. Thirty weeks after immunization, the immunized hamsters developed tubular adenocarcinoma at a higher rate (71.43%) than the non-immunized group (20.00%). This rate (20.00%) increased to 63.64% by week 39. The small foci cancer in the non-immunized group decreased in frequency from 80.00% (at week 30) to 36.36% (by week 39), suggesting the small foci cancer progressed to tubular adenocarcinoma during the 9-week interval. Most of the observed tubular adenocarcinoma was well differentiated. Nearly all hamsters that tested positive for cancer also tested positive for p53 immunostaining in the epithelia of the small bile ducts. The positive reaction for p53-immunostaining was localized in the rough endoplasmic reticulum, Golgi apparatus and perinuclear membranes. The electron micrographs of these positive p53-immunostained cells showed characteristics of early cancer. The detection of p53 in early cancer development makes it a candidate as a tumor marker.
Asunto(s)
Colangiocarcinoma/patología , Colangiocarcinoma/parasitología , Cocarcinogénesis , Inmunización/efectos adversos , Neoplasias Hepáticas Experimentales/patología , Neoplasias Hepáticas Experimentales/parasitología , Opisthorchis/inmunología , Animales , Antígenos Helmínticos/administración & dosificación , Antígenos Helmínticos/efectos adversos , Antígenos Helmínticos/inmunología , Carcinógenos , Colangiocarcinoma/inducido químicamente , Colangiocarcinoma/inmunología , Cricetinae , Dimetilaminas , Neoplasias Hepáticas Experimentales/inducido químicamente , Neoplasias Hepáticas Experimentales/inmunología , Masculino , Mesocricetus , Microscopía InmunoelectrónicaRESUMEN
The impact of opisthorchiasis on the manifestations of herpes simplex viruses type 2 (HSV-2) was studied. The parasites were established to tend to cause the body's hypersensitivity to HSV-2, particularly after repeated invasions and in summer, the process being characterized by urogenital and bile duct lesions coupled with high proliferation rate in their epithelium. Opisthorchiasis became a reservoir and an additional source of viral infection. There were also a definite damage to their reproductive system. The possible involvement of HSV-2 in the development of primary liver carcinoma in opisthorchiasis is discussed in the paper.
Asunto(s)
Herpes Genital/etiología , Herpesvirus Humano 2/patogenicidad , Neoplasias Hepáticas Experimentales/etiología , Opistorquiasis/complicaciones , Opisthorchis/patogenicidad , Animales , Conductos Biliares/patología , Cricetinae , Femenino , Herpes Genital/complicaciones , Herpes Genital/parasitología , Herpes Genital/patología , Herpes Genital/virología , Neoplasias Hepáticas Experimentales/parasitología , Neoplasias Hepáticas Experimentales/patología , Neoplasias Hepáticas Experimentales/virología , Masculino , Mesocricetus , Opistorquiasis/parasitología , Opistorquiasis/patología , Opistorquiasis/virología , Factores de TiempoRESUMEN
When inoculated with sporozoites of Plasmodium berghei, a line of hepatoma cells (HepG2-A16) derived from human liver supports the complete asexual developmental cycle of the exoerythrocytic stage. Parasites were shown to resemble parasites in vivo in hepatocytes. Subinoculation of merozoites into mice induced a red blood cell infection.
Asunto(s)
Neoplasias Hepáticas Experimentales/parasitología , Malaria/parasitología , Plasmodium berghei/parasitología , Animales , Eritrocitos/parasitología , Técnicas In Vitro , Ratones , Ratones EndogámicosRESUMEN
The attachment and entry of Plasmodium berghei sporozoites to cultured human lung fibroblast (WI38) or hepatoma (HepG2-A16) cells in vitro has been visualized using an immunoperoxidase technique coupled with light microscopy. Attachment and entry was substantially more frequent with HepG2-A16 cells, and appeared to be mediated by the Pb44 sporozoite surface protective antigen. When sporozoites were incubated with intact monoclonal antibodies to Pb44 or their monovalent Fab fragments, attachment was inhibited, suggesting that this technique may be an in vitro assay of protective immunity. Sporozoites appeared to enter cells actively, rather than by cell phagocytosis. Once within a membrane-bound vacuole, the sporozoite transformed into a trophozoite. It was suggested that Pb44 recognized a specific cell receptor, and that this technique may permit receptor characterization.