Asunto(s)

Humanos , Neisseria meningitidis/inmunología , Neisseria meningitidis/aislamiento & purificación , Neisseria meningitidis/ultraestructura , Vacunación/estadística & datos numéricos , Vacunación/instrumentación , Vacunación/métodos , Vacunación/normas

RESUMEN

The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequence for the 46 kDa and 28 kDa proteins matched that reported by others for class 1 and 5 proteins respectively, whereas the sequence (25 amino acids) for the 38 kDa (class 3) protein was similar to class 1 meningococcal proteins. The sequence for the 33 kDa (class 4) was unique and not homologous to any known protein.

Asunto(s)

Proteínas de la Membrana Bacteriana Externa/química , Neisseria meningitidis/ultraestructura , Secuencia de Aminoácidos , Brasil , Electroforesis en Gel de Poliacrilamida , Humanos , Datos de Secuencia Molecular , Neisseria meningitidis/aislamiento & purificación