RESUMEN
Anaerobic mollicutes were isolated from the intestinal content and the epithelium of the caecum and colon of 4 of 8 swine. All of the isolates belonged to the same species. This species was resistant to digitonin and polyanethol sulfonate and was able to ferment glucose, hemolyse erythrocytes and lyse horse serum. For testing the cholesterol requirement the strain 4 A 11 was selected which was independent of cholesterol. The agent was different in the immunofluorescence test from the mollicutes reported previously in swine and quite different in its nutritional requirements from cholesterol-independent mollicutes isolated from the rumen of cattle and sheep. Thus the isolated mollicutes seem to represent a new species.
Asunto(s)
Intestinos/microbiología , Mycoplasmatales/aislamiento & purificación , Animales , Células Cultivadas , Mycoplasmatales/crecimiento & desarrollo , Mycoplasmatales/metabolismo , PorcinosRESUMEN
Two species of obligately anaerobic mycoplasmas were the major components of a methanogenic glucose-limited enrichment culture. In pure culture, one of these organisms, tentatively named Anaeroplasma sp. strain London, was shown to be responsible for the fermentation of glucose to fatty acids, hydrogen, and carbon dioxide; the other mycoplasma was shown to produce methane from hydrogen and carbon dioxide and was named Methanoplasma elizabethii. This same methanogenic mycoplasma contained a low-molecular-weight fluorescent cofactor which had a maximum light absorbance at 430 nm. When both species of mycoplasmas were grown together on glucose, fermentation products included fatty acids and methane. For the first time, mycoplasmas are implicated as agents of anaerobic degradation and methanogenesis in a sewage sludge digester.
Asunto(s)
Glucosa/metabolismo , Metano/biosíntesis , Mycoplasma/metabolismo , Mycoplasmatales/metabolismo , Anaerobiosis , Dióxido de Carbono/metabolismo , Ácidos Grasos/biosíntesis , Hidrógeno/metabolismo , Mycoplasmatales/citología , Mycoplasmatales/crecimiento & desarrolloRESUMEN
The scanning electron microscope was utilized to observe the morphology of the thermophilic, acidophilic mycoplasma, Thermoplasma acidophilum. Upon examination of the surface morphology, the size and shape of this unusual mycoplasma revealed its similarity to the other mycoplasmas that have been investigated.