RESUMEN
We report an unusual case of anaphylaxis induced by the lysozyme-containing over-the-counter-drug Lysopaine®, which contains 20 mg lysozyme hydrochloride and 1.5 mg cetylpyridinium chloride, in a 9-year-old child with allergy to hen's egg as well as multiple IgE-mediated food allergies. The involvement of lysozyme was confirmed by positive skin prick tests for Lysopaine® and the presence of specific IgE against lysozyme. Our case highlights the importance of properly educating allergic patients to recognize allergens, even minor ones. Despite the presence of lysozyme in various food and drug products, it is not necessarily perceived as an allergenic protein by patients with egg allergy, and the labeling may be misleading, thereby exposing patients to potentially severe reactions.
Asunto(s)
Anafilaxia , Hipersensibilidad al Huevo , Niño , Humanos , Femenino , Animales , Hipersensibilidad al Huevo/complicaciones , Hipersensibilidad al Huevo/diagnóstico , Anafilaxia/diagnóstico , Anafilaxia/etiología , Muramidasa/efectos adversos , Pollos , Inmunoglobulina E , Alérgenos/efectos adversosRESUMEN
Uma área de pesquisa que vem ganhando muita atenção nos últimos anos é a nanomedicina, com especial atenção para os sistemas com entrega controlada de fármacos, ou drug delivery. Dentre as diversas nanopartículas utilizadas para este fim, destacam-se os sistemas formados por lipídeos e polímeros, como por exemplo os lipossomos e os cubossomos. Neste trabalho, é estudada a influência estrutural da lisozima e da curcumina, proteínas modelo. A lisozima é uma enzima antimicrobiana produzida por animais e que faz parte do sistema imunológico. Ela é uma hidrolase glicosídica que catalisa a hidrólise dos componentes da parede celular de bactérias gram-positivas. Esta hidrólise, por sua vez, compromete a integridade das paredes celulares, causando a lise (e como consequência a morte) das bactérias. Curcumina é um composto cristalino de cor amarelada brilhante, encontrada no caule da Curcuma longa (ou açafrão), que tem sido utilizada como corante ou até mesmo como aditivo alimentar. Este composto tem sido uma grande aposta no tratamento de doenças crônicas como inflamação, artrite, síndrome metabólica, doença hepática, obesidade, doenças neurodegenerativas e principalmente canceres, sendo também utilizada em estudos como potencial agente antibacteriano. O principal objetivo deste trabalho é construir sistemas nanoestruturados com potencial de atuarem como sistemas antimicrobianos, com a liberação controlada de ambos dos fármacos. Estes sistemas são compostos por cubossomos de fitantriol (PHY) em ausência e presença da lisozima, da curcumina e de suas combinações, a fim de analisar ação antimicrobiana conjunta da lisozima e da curcumina. As técnicas biofísicas utilizadas para caracterizar essas partículas são SAXS (espalhamento de raios-X em baixos ângulos), DLS (espalhamento dinâmico de luz), Cryo-TEM (criomicroscopia eletrônica de transmissão) e NTA (análise de rastreamento de nanopartículas). Foi possível verificar que as formulações lipídicas são eficazes na formação de estruturas cúbicas com estabilidade desejável. As nanopartículas cúbicas demonstraram alta capacidade de encapsulação da lisozima e da curcumina. A cinética de liberação desses medicamentos mostrou-se promissora, sugerindo que a encapsulação dos fármacos é eficaz, bem como a liberação controlada e direcionada. Duas linhagens de bactérias foram estudadas, sendo que a E. coli, não sofreu nenhum dano citotóxico, enquanto a Bacillus subtilis sim. Tal resultado indica o potencial antimicrobiano do sistema para alguns tipos de bactérias
An area of research that has gained significant attention in recent years is nanomedicine, with a particular focus on drug delivery systems. Among the various nanoparticles used for this purpose, lipid and polymer-based systems, such as liposomes and cubosomes stand out. This study investigate the structural influence of encapsulating lysozyme and curcumin, model compounds. Lysozyme is an antimicrobial enzyme produced by animals and is part of the immune system. It is a glycosidic hydrolase that catalyzes the hydrolysis of components in the cell walls of gram-positive bacteria. This hydrolysis compromises the integrity of cell walls, leading to the lysis (and consequently the death) of bacteria. Curcumin is a bright yellow crystalline compound found in the stem of Curcuma longa (or turmeric), commonly used as a dye or even as a food additive. It has been a significant focus in the treatment of chronic diseases such as inflammation, arthritis, metabolic syndrome, liver disease, obesity, neurodegenerative diseases, and especially cancers. It is also studied as a potential antibacterial agent. The main objective of this study is to construct nanostructured systems with the potential to act as antimicrobial agents, with controlled release of both drugs. These systems consist of phytantriol (PHY) cubosomes in the absence and presence of lysozyme, curcumin, and their combinations to analyze the joint antimicrobial action of lysozyme and curcumin. Biophysical techniques used for characterization include Small-Angle X-ray Scattering (SAXS), Dynamic Light Scattering (DLS), Cryo-Transmission Electron Microscopy (Cryo-TEM), and Nanoparticle Tracking Analysis (NTA). It was observed that lipid formulations are effective in forming cubic structures with desirable stability. Cubic nanoparticles have demonstrated a high encapsulation capacity for lysozyme and curcumin. The release kinetics of these drugs have shown promise, suggesting that drug encapsulation is effective, as well as their controlled and targeted release. Two bacterial strains were studied, with E. coli showing no cytotoxic damage, while Bacillus subtilis did. This result indicates the antimicrobial potential of the system against types of bacteria
Asunto(s)
Muramidasa/efectos adversos , Curcumina/efectos adversos , Aditivos Alimentarios/clasificación , Bacillus subtilis/clasificación , Preparaciones Farmacéuticas/análisis , Enfermedad Crónica/prevención & control , Microscopía Electrónica de Transmisión de Rastreo/métodos , Microscopía por Crioelectrón/métodos , Microscopía Electrónica de Transmisión/métodos , Colorantes/clasificación , Antiinfecciosos/efectos adversosRESUMEN
INTRODUCTION AND OBJECTIVES: It was urgent to explain the role of egg yolk allergen sensitization to the egg allergic population and we would evaluate the diagnostic value of allergen components in whole eggs, including egg white and egg yolk. MATERIALS AND METHODS: Firstly, we collected 99 positive and 21 negative sera against egg allergy. Then we used modified enzyme linked immunosorbent assay (ELISA) to survey specific IgE (sIgE) to all-proven and single component in eggs, Ovomucoid (Gal d 1), Ovalbumin (Gal d 2), Ovotransferrin (Gal d 3), Lysozyme C (Gal d 4), Serum Albumin (Gal d 5), and YGP42(Gal d 6) in allergic and non-allergic populations. Last but not least, we studied the sIgE reactivities to egg allergen components by receiver operating characteristic (ROC) analysis. RESULTS: Among egg-allergic individuals, nearly 10% were sensitized to five of six egg allergen components, and the cross-reaction frequency between two egg yolk allergens with Gal d 1 was about 30% in the groups diagnosed with egg allergy or non-allergy. The best component-combination diagnosis in egg allergy of Gal d 1+ Gal d 6 demonstrated the largest area under curve (AUC) of 0.994. CONCLUSIONS: Our results suggested that there were individual differences in allergenicity of different egg allergen components, especially in the samples negative to egg allergy diagnosed but sensitive to egg yolk components. It was indicated that component resolved diagnosis of egg yolk improved the value for egg allergy management indispensably.
Asunto(s)
Alérgenos/efectos adversos , Hipersensibilidad al Huevo/diagnóstico , Yema de Huevo/efectos adversos , Adolescente , Adulto , Alérgenos/inmunología , Estudios de Casos y Controles , Niño , Preescolar , Conalbúmina/efectos adversos , Conalbúmina/inmunología , Hipersensibilidad al Huevo/sangre , Hipersensibilidad al Huevo/inmunología , Clara de Huevo/efectos adversos , Yema de Huevo/inmunología , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Lactante , Masculino , Muramidasa/efectos adversos , Muramidasa/inmunología , Ovalbúmina/efectos adversos , Ovalbúmina/inmunología , Ovomucina/efectos adversos , Ovomucina/inmunología , Adulto JovenRESUMEN
Combinations of human lysozyme (hLYS) and antimicrobial peptides (AMPs) are known to exhibit either additive or synergistic activity, and as a result, they have therapeutic potential for persistent and antibiotic-resistant infections. We examined hLYS activity against Pseudomonas aeruginosa when combined with six different AMPs. In contrast to prior reports, we discovered that some therapeutically relevant AMPs manifest striking antagonistic interactions with hLYS across particular concentration ranges. We further found that the synthetic AMP Tet009 can inhibit hLYS-mediated bacterial lysis. To the best of our knowledge, these results represent the first observations of antagonism between hLYS and AMPs, and they advise that future development of lytic enzyme and AMP combination therapies considers the potential for antagonistic interactions.
Asunto(s)
Péptidos Antimicrobianos/farmacología , Muramidasa/efectos adversos , Pseudomonas aeruginosa/crecimiento & desarrollo , Péptidos Antimicrobianos/química , Bacteriólisis/efectos de los fármacos , Antagonismo de Drogas , Humanos , Pseudomonas aeruginosa/efectos de los fármacosRESUMEN
Drug-induced hypersensitivity syndrome (DIHS), also referred to as drug reaction with eosinophilia and systemic symptoms (DRESS), is a severe hypersensitivity drug reaction affecting the skin and multiple internal organ systems. We report a 47-year-old man with DIHS/DRESS and comorbidities (fulminant type 1 diabetes mellitus, valsartan-induced photosensitivity, vitiligo and acute interstitial nephritis). Although acute interstitial nephritis usually appears in the early phase, his is a rare case of acute interstitial nephritis more than 2 years after the onset of DIHS/DRESS.
Asunto(s)
Síndrome de Hipersensibilidad a Medicamentos/complicaciones , Fatiga/tratamiento farmacológico , Nefritis Intersticial/diagnóstico , Acetaminofén/efectos adversos , Biopsia , Carbocisteína/efectos adversos , Claritromicina/efectos adversos , Creatinina/sangre , Síndrome de Hipersensibilidad a Medicamentos/tratamiento farmacológico , Síndrome de Hipersensibilidad a Medicamentos/patología , Quimioterapia Combinada/efectos adversos , Medicamentos Herbarios Chinos/efectos adversos , Humanos , Riñón/patología , Masculino , Persona de Mediana Edad , Muramidasa/efectos adversos , Nefritis Intersticial/sangre , Nefritis Intersticial/etiología , Nefritis Intersticial/patología , Prednisolona/uso terapéutico , Piel/patología , Factores de TiempoRESUMEN
This observational clinical study was composed of two substudies: a non-comparative one (n = 166), testing only lysozyme-based compounds (LBCs), and a comparative substudy (n = 275), testing both LBCs and bicarbonate-based local compounds (BBCs) on the healing of oral mucositis during radio- or chemotherapy. The density of ulcerations has decreased significantly after the treatment with lysozyme in both substudies. The density of ulcerations in the radiotherapy group was lower in patients treated with LBCs compared to patients treated with BBCs (p < 0.001). In the chemotherapy group, reduction of ulceration density was similar with both LBCs and BBCs. The LBCs reduced pain intensity during the intake of solid food and speech more than BBCs in both patient cohorts (p < 0.05). In the radiotherapy cohort, pain intensity when consuming liquid foods was reduced more with LBCs than with BBCs (p < 0.05). No adverse events were recorded. This study demonstrates the advantages of treating oral mucositis during radiotherapy or chemo-therapy with LBCs.
Asunto(s)
Muramidasa/efectos adversos , Muramidasa/uso terapéutico , Radioterapia/efectos adversos , Estomatitis/tratamiento farmacológico , Antineoplásicos/efectos adversos , Fármacos Dermatológicos/efectos adversos , Fármacos Dermatológicos/uso terapéutico , Femenino , Humanos , Masculino , Persona de Mediana Edad , Dolor/tratamiento farmacológico , Estomatitis/etiologíaRESUMEN
BACKGROUND: Chronic childhood malnutrition, as manifested by stunted linear growth, remains a persistent barrier to optimal child growth and societal development. Environmental enteric dysfunction (EED) is a significant underlying factor in the causal pathway to stunting, delayed cognitive development, and ultimately morbidity and mortality. Effective therapies against EED and stunting are lacking and further clinical trials are warranted to effectively identify and operationalize interventions. METHODS/DESIGN: A prospective randomized placebo-controlled parallel-group randomized controlled trial will be conducted to determine if a daily supplement of lactoferrin and lysozyme, two important proteins found in breast milk, can decrease the burden of EED and stunting in rural Malawian children aged 12-23 months old. The intervention and control groups will have a sample size of 86 subjects each. All field and laboratory researchers will be blinded to the assigned intervention group, as will the subjects and their caregivers. The percentage of ingested lactulose excreted in the urine (Δ%L) after 4 h will be used as the biomarker for EED and linear growth as the measure of chronic malnutrition (stunting). The primary outcomes of interest will be change in Δ%L from baseline to 8 weeks and to 16 weeks. Intention-to-treat analyses will be used. DISCUSSION: A rigorous clinical trial design will be used to assess the biologically plausible use of lactoferrin and lysozyme as dietary supplements for children at high risk for EED. If proven effective, these safe proteins may serve to markedly reduce the burden of childhood malnutrition and improve survival. TRIAL REGISTRATION: Clinicaltrials.gov, NCT02925026 . Registered on 4 October 2016.
Asunto(s)
Suplementos Dietéticos , Trastornos del Crecimiento/prevención & control , Trastornos de la Nutrición del Lactante/tratamiento farmacológico , Lactoferrina/uso terapéutico , Desnutrición/tratamiento farmacológico , Muramidasa/uso terapéutico , Esprue Tropical/tratamiento farmacológico , Factores de Edad , Estatura , Desarrollo Infantil , Protocolos Clínicos , Suplementos Dietéticos/efectos adversos , Femenino , Trastornos del Crecimiento/diagnóstico , Trastornos del Crecimiento/fisiopatología , Humanos , Lactante , Trastornos de la Nutrición del Lactante/diagnóstico , Trastornos de la Nutrición del Lactante/fisiopatología , Fenómenos Fisiológicos Nutricionales del Lactante , Análisis de Intención de Tratar , Lactoferrina/efectos adversos , Malaui , Masculino , Desnutrición/diagnóstico , Desnutrición/fisiopatología , Muramidasa/efectos adversos , Estado Nutricional , Estudios Prospectivos , Proyectos de Investigación , Esprue Tropical/diagnóstico , Esprue Tropical/fisiopatología , Factores de Tiempo , Resultado del TratamientoRESUMEN
Traditional herbal remedies stimulate and modulate the immune system, and it is thought that their glycoproteins and polysaccharides are responsible for this activity. We prepared crude water, protein, and polysaccharide extracts from Atractylodes macrocephala Koidz, Helianthus annuus L., Scutellaria barbata D. Don, and Hedyotis diffusa Willd, respectively, and compared their immune-stimulating activities in vitro and in vivo. All protein and polysaccharide samples of the plants led to greater lymphocyte proliferation and TNF-α and IL-6 production in cultured splenocytes than did the crude water extracts at the same concentrations tested. In addition, the protein and polysaccharide samples did not contain lectin- or lipopolysaccharide-like molecules, so glycoproteins were deduced to be responsible for the lymphocyte stimulation. Oral administration with each of the samples enhanced the hen egg-white lysozyme (HEL)-specific humoral immune and lymphocyte proliferative responses in HEL low-responder C57BL/6 mice. Splenocytes from the mice fed the samples showed significantly greater increases in the level of IFN-γ, but not IL-4, after stimulation with HEL compared with that from the untreated control. However, higher increases in HEL-specific IgG1, IgG2b, and IgG3 rather than IgG2a were found in the mice fed the samples. These results indicate that the sample-mediated enhancement of anti-HEL-specific humoral immune responses was due to the stimulation of B lymphocytes rather than a selective priming of helper T cell populations. Collectively, we suggest that glycoproteins and/or polysaccharides of traditional herbal remedies enhance cellular and humoral immune response induction and thus could be useful for patients who need enhanced immune function.
Asunto(s)
Glicoproteínas/farmacología , Factores Inmunológicos/farmacología , Activación de Linfocitos/efectos de los fármacos , Extractos Vegetales/farmacología , Plantas Medicinales/química , Polisacáridos/farmacología , Animales , Linfocitos B/efectos de los fármacos , Linfocitos B/inmunología , Células Cultivadas , Inmunidad Celular/efectos de los fármacos , Inmunidad Humoral/efectos de los fármacos , Inmunoglobulina G/inmunología , Interleucina-4/inmunología , Masculino , Ratones , Ratones Endogámicos C57BL , Muramidasa/efectos adversos , Muramidasa/inmunología , Linfocitos T Colaboradores-Inductores/efectos de los fármacos , Linfocitos T Colaboradores-Inductores/inmunologíaRESUMEN
Angiotensin-converting enzyme (ACE) inhibitors are important agents in blood pressure (BP) management. It was recently shown that the egg-protein hydrolysate NWT-03 inhibited ACE in Zucker diabetic fatty rats. We therefore designed a dose-finding study to assess the effects of 1, 2 and 5 g NWT-03 on daytime, 36-h, and night-time systolic and diastolic BP (SBP and DBP) in ninety-two generally healthy subjects with normal BP (n 29), high-normal BP (n 34) or mild hypertension (n 29). The study had a cross-over design with six treatment arms (1 g NWT-03 or placebo in period 1 and placebo or 1 g NWT-03 in period 2, 2 g NTW-03 or placebo in period 1 and placebo or 2 g NWT-03 in period 2, or 5 g NTW-03 or placebo in period 1 and placebo or 5 g NTW-03 in period 2). A comparable number of subjects from each BP class were included in each study arm. Duration of both treatments in each arm was 7 d, separated by 5-d wash-out periods. BP was measured with an ambulatory BP monitor before and after the treatments. In mild-hypertensive subjects, 2 g NWT-03 significantly decreased daytime SBP (7·9 mmHg; P=0·006), daytime DBP (4·2 mmHg; P=0·009), 36-h SBP (6·9 mmHg; P=0·015) and 36-h DBP (3·5 mmHg; P=0·035) compared with placebo subjects. In addition, in mild-hypertensive subjects, 5 g NWT-03 significantly decreased night-time SBP (14·8 mmHg; P=0·008) and night-time DBP (8·4 mmHg; P=0·020) compared with that in placebo subjects. To conclude, we found that 2 g NWT-03 lowered daytime and 36-h BP in subjects with mild hypertension, and 5 g NWT-03 lowered night-time BP in subjects with mild hypertension. As no dose-response relationship was evident, these results should be interpreted with care, and additional studies are needed.
Asunto(s)
Inhibidores de la Enzima Convertidora de Angiotensina/uso terapéutico , Antihipertensivos/uso terapéutico , Suplementos Dietéticos , Hipertensión/dietoterapia , Muramidasa/uso terapéutico , Prehipertensión/dietoterapia , Hidrolisados de Proteína/uso terapéutico , Adulto , Inhibidores de la Enzima Convertidora de Angiotensina/administración & dosificación , Inhibidores de la Enzima Convertidora de Angiotensina/efectos adversos , Antihipertensivos/administración & dosificación , Antihipertensivos/efectos adversos , Monitoreo Ambulatorio de la Presión Arterial , Ritmo Circadiano , Estudios Cruzados , Suplementos Dietéticos/efectos adversos , Femenino , Humanos , Hipertensión/fisiopatología , Masculino , Persona de Mediana Edad , Muramidasa/administración & dosificación , Muramidasa/efectos adversos , Prehipertensión/fisiopatología , Hidrolisados de Proteína/administración & dosificación , Hidrolisados de Proteína/efectos adversos , Reproducibilidad de los Resultados , Índice de Severidad de la EnfermedadRESUMEN
PURPOSE: This multicenter, randomized, parallel group study analyzed the effectiveness of an experimental oral gel, a commercially available oral rinse and a commercially available mouth spray versus water alone at relieving self-reported symptoms of dry mouth over a 28-day home use treatment period. The effects of the study treatments on dry mouth-related quality of life (QoL) were also investigated. METHODS: Eligible subjects were stratified by dry mouth severity (mild, moderate or severe) and randomized to receive one of the study treatments. Prior to first use they completed a questionnaire designed to assess their baseline dry mouth-related QoL. Following first use and on Day 8 (2 hours post-treatment only) and Day 29, subjects completed the modified Product Performance and Attributes Questionnaire (PPAQ) I at 0.5, 1, 2 and 4 hours post-treatment. Subjects further assessed treatment performance using the PPAQ II questionnaire on Days 8 and 29 and the dry mouth-related QoL questionnaire on Day 29. RESULTS: In 396 randomized subjects almost all comparisons of responses to PPAQ I, including those for the primary endpoint (response to PPAQ I Question 1 'Relieving the discomfort of dry mouth' after 2 hours on Day 29), were statistically significant in favor of active treatment groups versus water (P < 0.05). All comparisons of responses to PPAQ II on Days 8 and 29 were statistically significant in favor of active treatments versus water (P < 0.05). Moreover, nearly all comparisons for dry mouth-related QoL scores on Day 29 were statistically significant in favor of the active treatments versus water. All the dry mouth management strategies in this trial were well tolerated.
Asunto(s)
Glucosa Oxidasa/uso terapéutico , Lactoperoxidasa/uso terapéutico , Muramidasa/uso terapéutico , Xerostomía/prevención & control , Adulto , Aerosoles , Anciano , Anciano de 80 o más Años , Actitud Frente a la Salud , Combinación de Medicamentos , Femenino , Estudios de Seguimiento , Geles , Glucosa Oxidasa/administración & dosificación , Glucosa Oxidasa/efectos adversos , Humanos , Lactoperoxidasa/administración & dosificación , Lactoperoxidasa/efectos adversos , Lubricantes/administración & dosificación , Lubricantes/efectos adversos , Lubricantes/uso terapéutico , Masculino , Persona de Mediana Edad , Antisépticos Bucales/uso terapéutico , Muramidasa/administración & dosificación , Muramidasa/efectos adversos , Calidad de Vida , Autoimagen , Autoinforme , Resultado del Tratamiento , Agua , Xerostomía/clasificación , Xerostomía/psicologíaRESUMEN
BACKGROUND: Mucolytic agents are often used in Japan to ease excessive mucus production in patients with chronic obstructive pulmonary disease (COPD) or bronchial asthma (BA); the treatment ameliorates dyspnea and improves quality of life (QOL). AIM: Efficacy and safety of lysozyme hydrochloride (LYS), an oral mucolytic enzyme preparation, for patients with COPD or BA were investigated. PATIENTS AND METHODS: This study was a placebo-controlled, double-blind, randomized, cross-over design. Twenty-four patients with COPD and twenty-four patients with BA were enrolled. LYS or placebo was administered for 28 days in each treatment period, with a 28-day washout between the first and second treatment periods. The results of spirometry, impulse oscillometry system (IOS) examination, fractional exhaled nitric oxide (FeNO) measurement, as well as the changes in the subjective symptoms, were evaluated after the treatment period. RESULTS: On spirometry, airway function (FEV1) improved in patients with COPD after administration of LYS (LYS vs placebo: 0.08 L vs 0.029 L, p = 0.030). Similar trends were also found in %FEV1 in COPD patients. On IOS examination, resistance of the respiratory system at 5 Hz levels was significantly improved only in patients with COPD (LYS vs placebo: -0.455 cm H2O/L/s vs 0.095 cmH2O/L/s, p = 0.012). Similar trends were found in terms of the resistance of the respiratory system at 20 Hz, and of the reactance area. In the COPD assessment test, subjective symptoms also significantly improved in patients with COPD during the LYS treatment period (improvement rates-LYS vs. placebo: 69.6% vs. 39.1%; p = 0.022). A similar effect of LYS was not seen in BA patients. CONCLUSION: LYS, a mucolytic agent, has capability to improve the function of peripheral airways in patients with COPD, which leads to improvements of the patients' symptoms and QOL.
Asunto(s)
Asma/tratamiento farmacológico , Expectorantes/administración & dosificación , Muramidasa/administración & dosificación , Enfermedad Pulmonar Obstructiva Crónica/tratamiento farmacológico , Anciano , Anciano de 80 o más Años , Asma/fisiopatología , Estudios Cruzados , Método Doble Ciego , Disnea/tratamiento farmacológico , Disnea/etiología , Expectorantes/efectos adversos , Expectorantes/farmacología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Muramidasa/efectos adversos , Muramidasa/farmacología , Óxido Nítrico/metabolismo , Enfermedad Pulmonar Obstructiva Crónica/fisiopatología , Calidad de Vida , Espirometría , Esputo/metabolismo , Resultado del TratamientoRESUMEN
The mucosa of the esophagus, the stomach, the small intestine, the large intestine and rectum are unremittingly challenged by adverse micro-environmental factors, such as ingested pathogenic and non-pathogenic bacteria, and harsh secretions with digestive properties with disparate pH, as well as bacteria and secretions from upstream GI organs. Despite the apparently inauspicious mixture of secretions and bacteria, the normal GI mucosa retains a healthy state of cell renewal. To by-pass the tough microenvironment, the epithelia of the GI react by speeding-up cell exfoliation, by increasing peristalsis, eliminating bacteria through secretion of plasma cell-immunoglobulins and by increasing production of natural antibacterial enzymes (lysozyme) and host defense peptides (defensin-5). Lysozyme was recently found up-regulated in Barrett's esophagitis, in chronic gastritis, in gluten-induced atrophic duodenitis (celiac disease), in collagenous colitis, in lymphocytic colitis and in Crohn's colitis. This up-regulation is a response directed towards the special types of bacteria thriving in the microenvironment in each of the aforementioned clinical inflammatory maladies. The purpose of that up-regulation is to protect the mucosa affected by the ongoing chronic inflammation. Bacterial antibiotic resistance continues to exhaust our supply of effective antibiotics. The future challenge is how to solve the increasing menace of bacterial resistance to anti-bacterial drugs. Further research on natural anti-bacterial enzymes such as lysozyme, appears mandatory.
Asunto(s)
Esófago de Barrett/patología , Enfermedad Celíaca/patología , Colitis Colagenosa/patología , Colitis Linfocítica/patología , Colitis Ulcerosa/patología , Enfermedad de Crohn/patología , Gastritis/patología , Enfermedades Gastrointestinales/patología , Muramidasa/efectos adversos , Humanos , InflamaciónRESUMEN
Proteinaceous products are widely used as fining agents during winemaking to remove unwanted insoluble particles and undissolved microscopic particles (colloidal material) from the must or wine to improve stability. Some of them (egg white, caseinates, and fish gelatine) have allergenic potential and the presence of their residues in the final product could represent a risk for allergic individuals. Moreover, lysozyme (an egg allergen) is included among wine additives to control the fermentation processes and avoid spoiling during winemaking. The aim of this paper is to review the experimental/clinical data on the use of allergenic products in enology and the measurement of relative risk for sensitized subjects. In addition, methods developed specifically for the quantification of allergenic residues in must and wine are described.
Asunto(s)
Alérgenos , Análisis de los Alimentos , Inocuidad de los Alimentos , Muramidasa , Vino/análisis , Alérgenos/análisis , Alérgenos/química , Humanos , Muramidasa/efectos adversos , Muramidasa/análisis , Muramidasa/químicaRESUMEN
Potential residues of the potent allergen lysozyme used as a microbial stabilizing agent in wine production might pose a serious health thread to susceptible individuals. Therefore, EU legislation requires the labeling of the allergenic agent, if it is present in the final product. To allow for product testing, an indirect ELISA method to be specifically used in wine analysis was developed and validated. Furthermore, trial wines treated with defined amounts of lysozyme were subjected to an array of different filtration and other enological processing regimes in order to evaluate their potential to deplete the allergen content of the wines. By these means, processing methods ought to be identified that can be integrated in a good manufacturing practice guideline to enable wine producers to utilize lysozyme in their cellars and still provide wines free of allergenic residues. However, among the enological procedures under scrutiny, only bentonite fining proved to be capable of significantly reducing the allergenic residues.
Asunto(s)
Alérgenos/análisis , Aditivos Alimentarios/análisis , Contaminación de Alimentos/prevención & control , Manipulación de Alimentos , Inspección de Alimentos/métodos , Muramidasa/análisis , Vino/análisis , Bentonita/química , Proteínas Dietéticas del Huevo/efectos adversos , Proteínas Dietéticas del Huevo/análisis , Proteínas Dietéticas del Huevo/antagonistas & inhibidores , Ensayo de Inmunoadsorción Enzimática , Unión Europea , Fermentación , Filtración , Aditivos Alimentarios/química , Hipersensibilidad a los Alimentos/etiología , Hipersensibilidad a los Alimentos/prevención & control , Alemania , Humanos , Viabilidad Microbiana , Muramidasa/efectos adversos , Muramidasa/antagonistas & inhibidores , Saccharomyces cerevisiae/crecimiento & desarrollo , Vino/microbiología , Vino/normasRESUMEN
PURPOSE: Lysozyme, obtained from egg white, is a potential food allergen used in the dairy industry to prevent late blowing of the loaf caused by the outgrowth of clostridial spores (Cl. butyricum and Cl. tyrobutyricum) during cheese aging. The aim of this study was to evaluate the possible correlation between egg protein allergy in pediatric age and sensitization to egg lysozyme, used for the preparation of Grana Padano cheese. METHODS: The tolerability of Grana Padano cheese has been evaluated in pediatric patients allergic to egg proteins through an oral provocation test with increasing amounts of cheese containing, or not, lysozyme at 12 and 24 months of aging. RESULTS: When lysozyme-sensitized children received 12-months aged and lysozyme-containing cheese, several immediate and late adverse reactions such as itching, abdominal pain, vomiting, nausea, dermatitis, rhinitis, bronchial asthma, urticaria, and angioedema were seen in 5 out of 21 subjects; only 1 out of 21 children showed an adverse reaction after challenge with 24-months-ripened lysozyme-containing cheese. CONCLUSIONS: There is a possible relationship between the severity of allergic reactions and the lysozyme-specific IgE level in blood. In particular vomiting, hypotension, and abdominal pain were present when IgE level was higher than 7 kU/L. A ripening time of 24 months may reduce allergy problems when lysozyme-containing cheese is given to sensitized subjects, probably due to the hydrolysis of antigenic epitopes during aging.
Asunto(s)
Antígenos/efectos adversos , Queso/efectos adversos , Dieta/etnología , Hipersensibilidad al Huevo/inmunología , Manipulación de Alimentos , Muramidasa/efectos adversos , Adolescente , Antígenos/metabolismo , Queso/análisis , Queso/microbiología , Niño , Preescolar , Clostridium butyricum/crecimiento & desarrollo , Clostridium tyrobutyricum/crecimiento & desarrollo , Hipersensibilidad al Huevo/sangre , Hipersensibilidad al Huevo/dietoterapia , Hipersensibilidad al Huevo/fisiopatología , Femenino , Fermentación , Inspección de Alimentos , Humanos , Inmunoglobulina E/análisis , Italia , Masculino , Muramidasa/metabolismo , Índice de Severidad de la Enfermedad , Factores de TiempoRESUMEN
The objective of the present study was to estimate the efficacy of laripront intended for the treatment of inflammatory diseases of the laryngopharynx in the children. Available for the observation were 50 patients aged between 4 and 14 years suffering from the following ENT pathologies: adenoiditis, lacunar tonsillitis, acute laryngitis, chronic tonsillitis, oropharyngeal candidiasis, chronic hypertrophic pharyngitis, atrophic pharyngolaryngitis after the chemical burn of the mouse cavity and laryngopharynx or in the case of gastroesophageal reflux disease. All the patients enjoyed the positive outcome of the treatment that was especially efficacions in the patients with acute pathologies. No adverse effects of the treatment were documented.
Asunto(s)
Decualinio , Hipofaringe , Muramidasa , Dolor , Infecciones del Sistema Respiratorio , Enfermedad Aguda , Adolescente , Antiinfecciosos Locales/administración & dosificación , Antiinfecciosos Locales/efectos adversos , Niño , Preescolar , Enfermedad Crónica , Decualinio/administración & dosificación , Decualinio/efectos adversos , Combinación de Medicamentos , Monitoreo de Drogas , Sinergismo Farmacológico , Femenino , Humanos , Hipofaringe/efectos de los fármacos , Hipofaringe/microbiología , Masculino , Muramidasa/administración & dosificación , Muramidasa/efectos adversos , Dolor/tratamiento farmacológico , Dolor/etiología , Infecciones del Sistema Respiratorio/clasificación , Infecciones del Sistema Respiratorio/complicaciones , Infecciones del Sistema Respiratorio/tratamiento farmacológico , Infecciones del Sistema Respiratorio/microbiología , Comprimidos , Resultado del TratamientoRESUMEN
Subpopulations of pathogenic or nonpathogenic Th17 cells were reported to develop when presensitized CD4 cells were activated with their target Ag during polarization by either IL-23 or IL-6 and TGF-ß, respectively. In this study, we generated two Th17 subpopulations by using a system in which naive CD4 cells from TCR transgenic mice specific to hen egg lysozyme (HEL) are polarized with IL-6/TGF-ß and, concurrently, are activated either with HEL presented by APCs, or with anti-CD3/CD28 Abs. Only the former cells were pathogenic, inducing inflammation in eyes expressing HEL. Naive CD4 cells activated by the anti-CD3/CD28 Abs acquired pathogenicity, however, when cocultured with HEL/APC. Importantly, the naive CD4 cells did not acquire pathogenicity when cocultured with APCs stimulated with LPS or when separated from the HEL-presenting cells by a semipermeable membrane. Unlike with presensitized Th17, soluble IL-23 does not participate in pathogenicity acquisition by naive CD4 cells; no pathogenicity was induced by adding IL-23 to cultures activated with anti-CD3/CD28 Abs. Furthermore, Abs against IL-23 or IL-23R did not inhibit acquisition of pathogenicity in cultures of naive CD4 cells activated by HEL/APC. Our data thus show that, unlike presensitized CD4 cells, naive CD4 cells polarized toward Th17 phenotype acquire pathogenicity only by direct interaction with APCs presenting the Ag, with no apparent involvement of soluble IL-23. We suggest that the Th17 lymphocytes derived from naive CD4 cells participate in pathogenic and other immune processes, along with the IL-23-dependent Th17 cells.
Asunto(s)
Células Presentadoras de Antígenos/inmunología , Linfocitos T CD4-Positivos/inmunología , Comunicación Celular/inmunología , Linaje de la Célula/inmunología , Muramidasa/metabolismo , Células Th17/inmunología , Animales , Células Presentadoras de Antígenos/metabolismo , Linfocitos T CD4-Positivos/enzimología , Linfocitos T CD4-Positivos/patología , Células Cultivadas , Técnicas de Cocultivo , Células Dendríticas/inmunología , Oftalmopatías/enzimología , Oftalmopatías/inmunología , Oftalmopatías/patología , Inflamación/enzimología , Inflamación/inmunología , Inflamación/patología , Interleucina-23/metabolismo , Ratones , Ratones Transgénicos , Muramidasa/efectos adversos , Muramidasa/inmunología , Células Th17/enzimología , Células Th17/patologíaRESUMEN
OBJECTIVE: Lysozyme is an enzyme that hydrolyzes bacterial peptidoglicans. For this reason, it is used in cheese manufacturing in order to prevent a defect of long-ripened hard cheese called "late blowing" due to the outgrowth of spores of Clostridium tyrobutyricum and Clostridium butyricum. Moreover, germination of Listeria monocytogenes spores into vegetative cells is also sensitive to lysozyme. The enzyme can be an allergenic molecule, and for this reason there are concerns about its use in food industry. The immunological and clinical response of consumption of lysozyme-containing cheese has been evaluated in 25 egg-sensitive subjects with or without lysozyme sensitization. METHODS: A total of 25 egg-sensitive subjects were enrolled in this study. All the subjects were already treated for egg-sensitization and presented a positive skin prick test. All the subjects had a body mass index ≤ 25 kg/m(2) and were in the age range of 20-50 years. Each subject was studied twice and received randomly 30 g of Grana Padano (containing lysozyme) or TrentinGrana cheese (lysozyme-free) of two different aging periods: 16 or 24 months. A washout period of 1 week between each cheese intake was adopted. Blood samples were taken in fasting conditions and 1 hour after cheese intake and IgA, total IgE, and lysozyme-, ovomucoid-, and ovalbumin-specific IgE were measured. RESULTS: No adverse reactions were observed in both groups of patients after cheese samples were given. Lysozyme did not determine any variation of specific IgE compared with basal level. In lysozyme-sensitive patients a significant relationship between IgA and lysozyme-specific IgE was observed when lysozyme-containing cheese was given, confirming that lysozyme can pass the gut barrier. CONCLUSIONS: Neither adverse events nor immunological responses were observed after ingestion of cheese containing lysozyme. However, the immunological properties of peptides deriving from cheese protein hydrolysis need to be clarified, as does the effect of lysozyme on bacterial proteolytic activity.
Asunto(s)
Queso/análisis , Queso/microbiología , Hipersensibilidad al Huevo/inmunología , Hipersensibilidad a la Leche/inmunología , Muramidasa/efectos adversos , Adulto , Alérgenos/inmunología , Índice de Masa Corporal , Clostridium butyricum/crecimiento & desarrollo , Clostridium butyricum/aislamiento & purificación , Clostridium tyrobutyricum/crecimiento & desarrollo , Clostridium tyrobutyricum/aislamiento & purificación , Método Doble Ciego , Humanos , Inmunoglobulina A/sangre , Inmunoglobulina E/sangre , Listeria monocytogenes/crecimiento & desarrollo , Listeria monocytogenes/aislamiento & purificación , Persona de Mediana Edad , Muramidasa/sangre , Muramidasa/inmunología , Ovomucina/sangre , Ovomucina/inmunología , Pruebas Cutáneas , Esporas Bacterianas/crecimiento & desarrollo , Esporas Bacterianas/inmunología , Adulto JovenRESUMEN
The Drug Allergy Committee of the Spanish Society of Allergology and Clinical Immunology reviewed the allergenic potential of several substances of food origin that are found in the composition of some drugs. Despite recent legislation on labeling, many labels do not clearly state whether the drug contains raw material (active ingredients, excipient, or other manufacturing intermediate) with an origin in any of the substances in the list of the 14 groups of food allergens that are subject to mandatory declaration. The objective of legislation is that the drug package, the Summary of Product Characteristics, and the patient information leaflet clearly state the food content in order to improve the safety of allergic patients. Therefore, any food or allergen derivative that must be declared should be clearly stated on the drug label. Of all the evaluated products, egg and milk derivatives are the most frequently discussed in literature reviews. The natural or synthetic origin of potentially allergenic substances such as lysozyme, casein, lactose, albumin, phosphatide, and aromatic essences should be clearly stated. Providing this information has 2 clear advantages. First, allergic reactions to drugs in patients with food allergy could be avoided (if the substances have a natural origin). Second, prescription would improve by not restricting drugs containing synthetic substances (which do not usually induce allergic reactions).
Asunto(s)
Hipersensibilidad a las Drogas/etiología , Aditivos Alimentarios/efectos adversos , Hipersensibilidad a los Alimentos/etiología , Glucosamina/efectos adversos , Humanos , Lactosa/efectos adversos , Muramidasa/efectos adversos , Ovalbúmina/efectos adversos , Propofol/efectos adversos , EspañaRESUMEN
There are over 200 food additives that have been approved in Finland. They are being used in order to improve preservability, flavor, appearance and texture. Some of them may cause hypersensitivity reactions, the most common being anaphylactic reactions, urticaria and exacerbation of asthma. Such reactions are, however, very rare. Anaphylactic reactions and other symptoms have resulted from carmine, lysozyme, acetic acid and acetates, gums of plant origin, sulfites and tartrazine.