RESUMEN
Visando investigar qualitativa e quantitativamente a participacao da microbiota psicrotrofila e patogenica em carcacas de frango durante e apos o processamento industrial, foram examinadas cento e oitenta unidades de amostras coletadas ao longo do processo em uma industria do Estado de Santa Catarina (Brasil). Numa segunda etapa, foi estudado o comportamento de cinco sanificantes de uso comercial, objetivando verificar a eficiencia dos mesmos na extensao da vida util de carcacas de frango refrigeradas. As contagens medias de microrganismos aerobios em carcacas de frango no inicio do processamento apresentaram valores oscilando entre '10 POT.4'e '10 POT.6' ufc/'cm POT.2' com incubacao a 7 graus centigrados e '10 POT.5' e > '10 POT.6' ufc/'cm POT.2' quando incubadas a 20 graus centigrados e trinta e cinco graus centigrados, respectivamente. Estes valores nao foram muito diferentes daqueles obtidos nas analises do produto final
Asunto(s)
Animales , Pollos , Conservación de Alimentos/normas , Desinfectantes , Enterobacteriaceae/análisis , Análisis de los Alimentos , Microbiología de Alimentos , Industria de Procesamiento de Alimentos , Moraxella/análisis , Pseudomonas/análisis , Refrigeración , Salmonella/análisis , Vibrionaceae/análisis , Contaminación de Alimentos , Tecnología de Alimentos , Aves de CorralRESUMEN
Six monoclonal antibodies (MAbs) against Moraxella bovis GF 9 were used to quantify haemagglutinating adhesins of 16 strains of this organism. The amount of each MAb necessary to inhibit one haemagglutinating unit of each strain varied between 4 and 0.007 times that required by strain GF 9. Five strains reacted with six MAbs, one with five, two with four, one with three, two with two and three with none. The procedures used enabled to detect dominant strains candidates for vaccines.
Asunto(s)
Anticuerpos Monoclonales , Hemaglutininas/análisis , Moraxella/análisis , Animales , Anticuerpos Monoclonales/inmunología , Bovinos , Enfermedades de los Bovinos/microbiología , Epítopos/análisis , Pruebas de Inhibición de Hemaglutinación , Hemaglutininas/inmunología , Hibridomas , Queratoconjuntivitis Infecciosa/microbiología , Moraxella/inmunologíaRESUMEN
Lipopolysaccharide from Moraxella bovis was isolated and its components characterized by paper and gas-liquid chromatography and mass spectrometry. The endotoxin showed a small amount of protein and contained 2-keto-3-deoxyoctonic acid glucose, mannose, glucosamine, heptose and phosphate. Six fatty acids (palmitic, palmitoleic, margaric, stearic, oleic and arachidic acids) were also identified. Margaric acid was the most prevalent fatty acid present in the lipid fraction.