RESUMEN
Otite externa (OE) é o termo utilizado para definir a inflamação do conduto auditivo externo; esta doença possui diversas etiologias, ocorre em várias espécies e é particularmente frequente em cães. Os microrganismos da microbiota residente comumente estão envolvidos na etiopatogenia da OE, sendo apontados como agentes perpetuadores da doença. O objetivo deste estudo foi investigar o perfil microbiológico de cães com conduto auditivo saudável e com otite na região metropolitana do Recife. Com o auxílio de suabes estéreis foram coletadas amostras das orelhas direita e esquerda de 41 cães, sendo 11 com OE e 30 sem OE. Foi realizado o isolamento bacteriano e fúngico das amostras cultivadas; observou-se positividade em 80% dos cães com orelhas saudáveis e presença de mais de um microrganismo em 38 amostras (63,3%); já nos cães com OE, a positividade foi 95,3%, com infecção polimicrobiana em 77,3% das amostras. No que se refere aos gêneros bacterianos, o perfil de isolamento microbiológico foi idêntico entre os cães otopatas e sadios. Os microrganismos isolados foram Staphylococcus sp., Micrococcus, Bacillus sp., Streptococcus sp. e Malassezia sp.(AU)
Otitis externa (OE) is the term used to describe inflammation of the external ear canal. This disease has many etiologies, occurs in several species and is particularly common in dogs. The resident microbiota microorganisms are commonly involved in the OE etiopathogenesis, being frequently appointed as perpetuator agents. The aim of this study was to investigate the microbiological profile of dogs with healthy ears and of others with otitis in the metropolitan region of Recife, Pernambuco, Brazil. With the aid of sterile swabs, samples of right and left ear of 41 dogs, 11 with and 30 without OE, were collected. Bacterial and fungal isolation was performed with cultured samples; positivity was observed in 80% of animals with healthy ears, with the presence of more than one microrganism in 38 samples (63.3%), whereas in dogs with OE, the positivity was 95.3% with polymicrobial infection in 77.3% samples. With regard to the genus, the microbiological profile was identical between healthy and diseased dogs. The microrganisms isolated were Staphylococcus sp., Micrococcus, Bacillus sp., Streptococcus sp. and Malassezia sp.(AU)
Asunto(s)
Animales , Perros , Conducto Auditivo Externo/microbiología , Otitis Externa/microbiología , Staphylococcus/aislamiento & purificación , Malassezia/aislamiento & purificación , Micrococcus/aislamiento & purificación , Bacillus/aislamiento & purificación , Streptococcus/aislamiento & purificación , Enfermedades del Oído/veterinariaRESUMEN
Otite externa (OE) é o termo utilizado para definir a inflamação do conduto auditivo externo; esta doença possui diversas etiologias, ocorre em várias espécies e é particularmente frequente em cães. Os microrganismos da microbiota residente comumente estão envolvidos na etiopatogenia da OE, sendo apontados como agentes perpetuadores da doença. O objetivo deste estudo foi investigar o perfil microbiológico de cães com conduto auditivo saudável e com otite na região metropolitana do Recife. Com o auxílio de suabes estéreis foram coletadas amostras das orelhas direita e esquerda de 41 cães, sendo 11 com OE e 30 sem OE. Foi realizado o isolamento bacteriano e fúngico das amostras cultivadas; observou-se positividade em 80% dos cães com orelhas saudáveis e presença de mais de um microrganismo em 38 amostras (63,3%); já nos cães com OE, a positividade foi 95,3%, com infecção polimicrobiana em 77,3% das amostras. No que se refere aos gêneros bacterianos, o perfil de isolamento microbiológico foi idêntico entre os cães otopatas e sadios. Os microrganismos isolados foram Staphylococcus sp., Micrococcus, Bacillus sp., Streptococcus sp. e Malassezia sp.
Otitis externa (OE) is the term used to describe inflammation of the external ear canal. This disease has many etiologies, occurs in several species and is particularly common in dogs. The resident microbiota microorganisms are commonly involved in the OE etiopathogenesis, being frequently appointed as perpetuator agents. The aim of this study was to investigate the microbiological profile of dogs with healthy ears and of others with otitis in the metropolitan region of Recife, Pernambuco, Brazil. With the aid of sterile swabs, samples of right and left ear of 41 dogs, 11 with and 30 without OE, were collected. Bacterial and fungal isolation was performed with cultured samples; positivity was observed in 80% of animals with healthy ears, with the presence of more than one microrganism in 38 samples (63.3%), whereas in dogs with OE, the positivity was 95.3% with polymicrobial infection in 77.3% samples. With regard to the genus, the microbiological profile was identical between healthy and diseased dogs. The microrganisms isolated were Staphylococcus sp., Micrococcus, Bacillus sp., Streptococcus sp. and Malassezia sp.
Asunto(s)
Animales , Perros , Malassezia/aislamiento & purificación , Conducto Auditivo Externo/microbiología , Otitis Externa/microbiología , Staphylococcus/aislamiento & purificación , Bacillus/aislamiento & purificación , Micrococcus/aislamiento & purificación , Enfermedades del Oído/veterinaria , Streptococcus/aislamiento & purificaciónRESUMEN
Two raw sausages were prepared: a soft and a dry-ripened one, both by local traditional and industrial manufacturing practices. Sausages were packaged under a CO2/N2 atmosphere at different targeted activity water (aw) values: 0.96 and 0.92 (soft sausages) and 0.88 and 0.82 (dry-ripened sausages). Sausages were then stored at 5 °C for 42 days or at 12 °C for 240 days (soft and a dry-ripened sausages, respectively). The time-related changes in dominant microbiota, pH and biogenic amine contents during storage were determined. Tyramine was the most abundant biogenic amine in all the sausages. Biogenic amine levels were higher in dry-ripened sausages than in soft sausages at packaging. However, during refrigerated storage soft sausages were fermented and the levels of biogenic amines increased (P<0.05). At the end of storage, traditional soft sausages with 0.96 aw presented comparable levels of biogenic amines to traditional dry-ripened sausages.
Asunto(s)
Aminas Biogénicas/análisis , Embalaje de Alimentos/métodos , Almacenamiento de Alimentos , Productos de la Carne/análisis , Animales , Atmósfera , Recuento de Colonia Microbiana , Fermentación , Microbiología de Alimentos , Concentración de Iones de Hidrógeno , Micrococcus/crecimiento & desarrollo , Micrococcus/aislamiento & purificación , Staphylococcus/crecimiento & desarrollo , Staphylococcus/aislamiento & purificación , Porcinos , Temperatura , Tiramina/análisis , Agua/análisisRESUMEN
High-altitude wetlands (above 4200m) in the northwest of Argentina are considered pristine and extreme environments. Micrococcus sp. A1, H5, and V7, isolated from such environments, were shown to contain linear megaplasmids, designated pLMA1, pLMH5, and pLMV7, respectively. As known from linear plasmids of other actinomycetes, all three plasmids were resistant to lambda exonuclease treatment, which is consistent with having terminal proteins covalently attached to their 5' DNA ends. Electrophoretic mobility, Southern analysis, and restriction endonuclease patterns revealed pLMA1 and pLMH5 being indistinguishable plasmids, even though they were found in different strains isolated from two distant wetlands - Laguna Azul and Laguna Huaca Huasi. Analysis of 16S rDNA sequences of Micrococcus sp. A1, H5, and V7 suggested a close relationship to Micrococcus luteus. Typing of isolates was performed using fingerprint patterns generated by BOX-PCR. Plasmid-deficient strains, generated from Micrococcus sp. A1, showed a significantly decreased resistance level for erythromycin.
Asunto(s)
Micrococcus/genética , Plásmidos/genética , Altitud , Técnicas de Tipificación Bacteriana , Southern Blotting , ADN Bacteriano/genética , Eritromicina/farmacología , Exodesoxirribonucleasas/metabolismo , Pruebas de Sensibilidad Microbiana , Micrococcus/clasificación , Micrococcus/efectos de los fármacos , Micrococcus/aislamiento & purificación , Reacción en Cadena de la Polimerasa , Mapeo Restrictivo , HumedalesRESUMEN
Bacterial strains were isolated from contaminated waters, mud or soils. They are capable of growing in mineral medium with different chemicals as carbon source, such as aliphatic or aromatic hydrocarbons and polychlorinated biphenyls (PCBs). Most of these strains tolerate high concentrations (up to 30% v/v) of the xenobiotic substrates. This is particularly important for the development of fermenting processes to treat effluents or residues with a high content of contaminating compounds. An ion-specific potentiometric electrode (CO2) has been developed to measure CO2 production continuously. When the different strains were incubated in a mineral medium and in the presence of the corresponding substrate, a parallel between growth, substrate consumption and CO2 production was found. The developed system is suggested as an efficient and economical alternative to evaluate the potential of biodegradation by different microorganisms.
Asunto(s)
Alcaligenes/metabolismo , Dióxido de Carbono/análisis , Carbono/metabolismo , Electrodos , Hidrocarburos/metabolismo , Micrococcus/metabolismo , Potenciometría/instrumentación , Pseudomonas aeruginosa/metabolismo , Xenobióticos/metabolismo , Alcaligenes/aislamiento & purificación , Alcanos/metabolismo , Arocloros/metabolismo , Biodegradación Ambiental , Calibración , Dióxido de Carbono/metabolismo , Contaminantes Ambientales/metabolismo , Diseño de Equipo , Fermentación , Micrococcus/aislamiento & purificación , Pseudomonas aeruginosa/aislamiento & purificación , Microbiología del Suelo , Estireno/metabolismo , Tolueno/metabolismo , Microbiología del Agua , Contaminación Química del AguaRESUMEN
Bacterial strains were isolated from contaminated waters, mud or soils. They are capable of growing in mineral medium with different chemicals as carbon source, such as aliphatic or aromatic hydrocarbons and polychlorinated biphenyls (PCBs). Most of these strains tolerate high concentrations (up to 30 v/v) of the xenobiotic substrates. This is particularly important for the development of fermenting processes to treat effluents or residues with a high content of contaminating compounds. An ion-specific potentiometric electrode (CO2) has been developed to measure CO2 production continuously. When the different strains were incubated in a mineral medium and in the presence of the corresponding substrate, a parallel between growth, substrate consumption and CO2 production was found. The developed system is suggested as an efficient and economical alternative to evaluate the potential of biodegradation by different microorganisms.(AU)
Asunto(s)
RESEARCH SUPPORT, NON-U.S. GOVT , Alcaligenes/metabolismo , Carbono/metabolismo , Dióxido de Carbono/análisis , Electrodos , Hidrocarburos/metabolismo , Micrococcus/metabolismo , Potenciometría/instrumentación , Pseudomonas aeruginosa/metabolismo , Xenobióticos/metabolismo , Alcaligenes/aislamiento & purificación , Alcanos/metabolismo , Arocloros/metabolismo , Calibración , Dióxido de Carbono/metabolismo , Contaminantes Ambientales/metabolismo , Diseño de Equipo , Fermentación , Micrococcus/aislamiento & purificación , Pseudomonas aeruginosa/aislamiento & purificación , Microbiología del Suelo , Estireno/metabolismo , Tolueno/metabolismo , Microbiología del Agua , Contaminación Química del AguaRESUMEN
Estuda 29 macacos-prego (Cebus apella, Linnaeus, 1758), colhendo 50 amostras da mucosa oral, junto à transiçäo muco-gengival maxilar, com o auxílio de zaragatoas esterilizadas, embebidas em caldo Brain Heart Infusion (BHI). Todos os animais foram submetidos a exame clínico para avaliaçäo periodontal. As amostras obtidas foram cultivadas em meios apropriados: caldo simples, caldo BHI, e ágar sangue para o isolamento de cocos gram-positivos aeróbicos da família Micrococcaceae. Para sua classificaçäo utiliza as provas de catalase, Staphy-test (teste rápido para caracterizaçäo de Staphylococcus aureus) e sensibilidade à bacitracina identificando-se 73,1 por cento de Staphylococcus spp, 15,4 por cento de Staphylococcus aureus e 11,5 por cento de Micrococcus spp. As cepas isoladas foram testadas em relaçäo à sua suscetibilidade a antibióticos pela técnica de difusäo em ágar. Verifica para as cepas de Staphylococcus spp, 94,7 por cento de sensibilidade à cefalotina e resistência de 89,5 por cento à penicilina, 97,4 por cento à oxacilina, 55,3 por cento à tetraciclina, 57,9 por cento à clindamicina e 63,2 por cento à amoxilina. Do total de animais, 96,5 por cento apresenta formaçäo de placa bacteriana, 82,8 por cento de deposiçäo de cálculo dentário e 58,6 por cento quadro clínico de gengivite. Os dados obtidos demonstram que a cefalotina é o antibiótico para o qual as amostras de Staphylococcus spp estudadas apresentam, in vitro, maior grau de sensibilidade
Asunto(s)
Animales , Antibacterianos , Técnicas In Vitro , Pruebas de Sensibilidad Microbiana , Micrococcus/aislamiento & purificación , Staphylococcus/aislamiento & purificación , Mordeduras y Picaduras , Cebus , Salud Bucal , Farmacorresistencia Microbiana , Staphylococcus aureus/aislamiento & purificación , Salud Pública VeterinariaRESUMEN
Se investigó la relación entre concentraciones de ozono atmosférico, temperatura máxima, humedad relativa y viabilidad de la población microbiana aérea, al suroeste de la ciudad de México, zona conocida como la máxima contaminación por ozono. Se tomaron un total de 35 muestras, en el periodo comprendido entre septiembre de 1992 y abril de 1993, analizadas por duplicado con cuatro medios de cultivo selectivos para el conteo total, así como el aislamiento de bacterias Gram negativas, positivas y hongos. Se encontró que para la cuenta total de microorganismos, éstos sí son afectados en su viabilidad por concentraciones elevadas de ozono, no así los parámetros climáticos de temperatura máxima y humedad relativa. El análisis de correlación entre especies y concentración de ozono, mostró que únicamente las bacterias Gram positivas y Gram negativas, no así los hongos, son afectados en número y viabilidad por concentraciones elevadas de ozono; en estecaso, tampoco son afectadas por temperatura máxima y humedad relativa. Las especies más frecuentemente identificadas fueron Micrococcus sp., Staphylococcus aureus y Penicillium sp
Asunto(s)
Análisis del Aire , Muestras de Aire , Recuento de Colonia Microbiana , Contaminación Ambiental/análisis , Contaminación Ambiental/efectos adversos , Hongos/aislamiento & purificación , Humedad , Micrococcus/aislamiento & purificación , Ozono/análisis , Penicillium/aislamiento & purificación , Microbiología del Suelo , Staphylococcus aureus/aislamiento & purificaciónRESUMEN
Se presentan los datos clínicos, epidemiológicos, histopatológicos y microbiológicos de 100 varones jóvenes con queratólisis punteada de localización plantar. La dermatosis fue bilateral en 97 porciento, todos presentaron depresiones puntiformes y diferentes cambios de color. Por histopatología en 90 por ciento se demostraron filamentos y elementos bacilares y cocoides en capa córnea, más evidentes con PAS y Gomori-Grocott. En 46 porciento se aisló Micrococcus sedentarius. Se presenta este microorganismo como probable agente etiológico asociado a una flora bacteriana abundante y como factores predisponentes humedad, fricción y maceración.