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BACKGROUND: Abdominal wall weakness occurs when the strength of muscle decreases due to physiological reason or iatrogenic injury. However, the treatment of this disease is complicated. AIM: To study the therapeutic effect of acellular tissue matrix (ACTM), compared with the polypropylene mesh. METHODS: An abdominal wall weakness model was established in rabbits through motor nerves cutting. The polypropylene mesh and ACTM were implanted in the left and right abdomen sides, respectively. Mechanical testing of abdominal wall muscle and histology and scanning electron microscopy (SEM) evaluation of abdominal tissue explants were performed. RESULTS: In animal model establishment, the abdominal length of healthy and weakened abdominal wall was 17.0 ± 0.7 cm and 19.0 ± 1.2 cm, respectively (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (P=0.022), and the weak abdominal wall group showed a significant decrease of 1.116 ± 0.221 MPa in tensile stress (. CONCLUSION: The abdominal wall weakness model in rabbits was successfully established. ACTM is a promising biological material to be possibly further applied in clinical surgery in patients with abdominal wall weakness.
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Músculos Abdominales/cirugía , Pared Abdominal/cirugía , Materiales Biocompatibles/farmacología , Polipropilenos/farmacología , Músculos Abdominales/efectos de los fármacos , Músculos Abdominales/patología , Músculos Abdominales/ultraestructura , Pared Abdominal/patología , Dermis Acelular , Animales , Materiales Biocompatibles/uso terapéutico , Modelos Animales de Enfermedad , Humanos , Polipropilenos/uso terapéutico , Conejos , Mallas Quirúrgicas/normas , Resistencia a la TracciónRESUMEN
OBJECTIVE: The purpose of this study was to determine the effect size in measurable change of abdominal musculature morphology using ultrasonography in postpartum women within 1 month of a healthy, vaginal delivery. METHODS: One hundred fifty-six participants were recruited for this study. B-mode ultrasound imaging was used to measure abdominal muscle thickness on 80 nulliparous women and 76 mothers who had delivered within the past 4 weeks. Measures were taken for the upper and lower rectus abdominus, external and internal obliques, and transversus abdominus at rest. RESULTS: Statistically significant differences were found in the thickness of the rectus abdominus muscle at both sites; upper (P < .0001) and lower (P < .0001) as well as the internal oblique (P < .0001). All 3 muscles were thinner in postpartum participants (8.29 ± 1.83 mm, 8.89 ± 2.29 mm, and 7.06 ± 1.82 mm, respectively) within the first month of delivery than in controls (10.82 ± 1.93 mm, 11.13 ± 2.38 mm, and 8.36 ± 1.87 mm, respectively). Large effect sizes were found for the influence of pregnancy on the rectus muscle segments (1.35 for the upper rectus abdominus and 1.00 for the lower rectus abdominus) and a medium effect size for the internal oblique (0.71). No significant differences were observed in the remaining 2 muscles. CONCLUSION: This study showed that there are differences in morphology of the abdominal muscles in pregnant women vs nonpregnant controls. The large effect sizes reported may provide the basis for future studies examining relationships between morphology, functional change, and back pain during pregnancy.
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Músculos Abdominales/fisiología , Músculos Abdominales/ultraestructura , Contracción Muscular/fisiología , Periodo Posparto , Adulto , Femenino , Humanos , Paridad , Diafragma Pélvico/fisiología , Recto del Abdomen/diagnóstico por imagen , Recto del Abdomen/ultraestructura , Valores de Referencia , Ultrasonografía , Adulto JovenRESUMEN
BACKGROUND: Ultrasound (US)-guided transversus abdominis plane (TAP) block is widely-spread analgesic technique for the patients undergoing abdominal surgery and can be provided safely for the patients receiving anticoagulation therapy. We conducted a retrospective comparative trial of analgesic efficacy between the patient who received US-guided TAP block with postoperative continuous iv-fentanyl infusion (group T) and those who received epidural analgesia (group E). METHODS: Twenty three patients who had undergone abdominal aortic replacement were analyzed retrospectively. The number of patients in group T was 12, and those in group E was 11. The postoperative first ambulation day, the postoperative first oral feeding day, the length of hospital stay, the use of rescue analgesic medication, and the incidence of postoperative nausea and vomiting (PONV) were compared between the two groups. P<0.05 was considered significant RESULTS: There were no significant differences in baseline characteristics between the two groups. There were no significant differences in postoperative first ambulation day (P=0.97), the postoperative first oral feeding day (P=0.46), the length of hospital stay (P= 0.32), the number of times of rescue analgesic medication (P=0.55), and the incidence of PONV between the two groups. CONCLUSIONS: US-guided TAP block is a useful option as analgesic technique for patients undergoing abdominal aortic replacement
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Músculos Abdominales/inervación , Músculos Abdominales/ultraestructura , Analgesia Epidural , Aorta Abdominal/cirugía , Aneurisma de la Aorta Abdominal/cirugía , Implantación de Prótesis Vascular , Fentanilo/administración & dosificación , Bloqueo Nervioso/métodos , Dolor Postoperatorio/prevención & control , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Incidencia , Infusiones Intravenosas , Masculino , Persona de Mediana Edad , Náusea y Vómito Posoperatorios/epidemiología , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
One of the most dramatic examples of programmed cell death occurs during Drosophila metamorphosis, when most of the larval tissues are destroyed in a process termed histolysis. Much of our understanding of this process comes from analyses of salivary gland and midgut cell death. In contrast, relatively little is known about the degradation of the larval musculature. Here, we analyze the programmed destruction of the abdominal dorsal exterior oblique muscle (DEOM) which occurs during the first 24h of metamorphosis. We find that ecdysone signaling through Ecdysone receptor isoform B1 is required cell autonomously for the muscle death. Furthermore, we show that the orphan nuclear receptor FTZ-F1, opposed by another nuclear receptor, HR39, plays a critical role in the timing of DEOM histolysis. Finally, we show that unlike the histolysis of salivary gland and midgut, abdominal muscle death occurs by apoptosis, and does not require autophagy. Thus, there is no set rule as to the role of autophagy and apoptosis during Drosophila histolysis.
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Abdomen/crecimiento & desarrollo , Apoptosis , Drosophila melanogaster/crecimiento & desarrollo , Ecdisona/metabolismo , Metamorfosis Biológica , Músculos/metabolismo , Músculos/patología , Transducción de Señal , Abdomen/patología , Músculos Abdominales/enzimología , Músculos Abdominales/metabolismo , Músculos Abdominales/patología , Músculos Abdominales/ultraestructura , Animales , Autofagia , Caspasas/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/citología , Drosophila melanogaster/enzimología , Drosophila melanogaster/ultraestructura , Epistasis Genética , Larva/metabolismo , Larva/ultraestructura , Músculos/enzimología , Músculos/ultraestructura , Sarcómeros/metabolismo , Sarcómeros/ultraestructura , Factores de TiempoRESUMEN
The distribution and the ultrastructure of afferent fibers innervating the parietal peritoneum in the rat was studied with immunohistochemistry using an antiserum against the neuronal marker protein gene product 9.5. The immunoreactive fibers were distributed throughout the peritoneum. They generally ran straight and parallel to the intercostal nerves running in the abdominal muscles underlying the peritoneum. They sometimes branched and terminated by forming club-like endings. The number of nerve endings on the peritoneal surface was 3.25 ± 1.66 mm(-2) . Electron microscopic observations revealed both unmyelinated and myelinated nerve fibers. The unmyelinated fibers were thin and about 1 µm in diameter. Their endings formed slight swellings located just inside the peritoneal cell layer. The myelinated fibers often formed a bundle that was composed of two or three nerve fibers. Each myelinated fiber kept in contact with a Shwann cell and projected toward the peritoneal cavity. Finally, they penetrated the peritoneal cell layer to reach the peritoneal cavity. These fibers then made contacts with the peritoneal cells and became free from the myelin sheath. The ending had a club-like shape covered with collagen fibers, and contained many neurofilaments, a few mitochondria, but no synaptic vesicles. These results suggest that since the sensory endings are exposed at the peritoneal cavity, the sensory fibers are highly sensitive to somatic or nociceptive stimuli.
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Músculos Abdominales/ultraestructura , Terminaciones Nerviosas/ultraestructura , Fibras Nerviosas/ultraestructura , Neuronas Aferentes/ultraestructura , Peritoneo/ultraestructura , Músculos Abdominales/metabolismo , Animales , Biomarcadores/metabolismo , Técnicas para Inmunoenzimas , Masculino , Microscopía Electrónica , Terminaciones Nerviosas/metabolismo , Fibras Nerviosas/metabolismo , Neuronas Aferentes/metabolismo , Peritoneo/metabolismo , Ratas , Ratas Sprague-DawleyRESUMEN
OBJECTIVES: Although motor control exercises have been shown to be effective in the management of low back pain (LBP) the mechanism of action is unclear. The current study investigated the relationship between the ability to recruit transversus abdominis and clinical outcomes of participants in a clinical trial. METHODS: Ultrasonography was used to assess the ability to recruit transversus abdominis in a nested design: a sample of 34 participants with chronic LBP was recruited from participants in a randomised controlled trial comparing the efficacy of motor control exercise, general exercise and spinal manipulative therapy. Perceived recovery, function, disability and pain were also assessed. RESULTS: Participants with chronic LBP receiving motor control exercise had a greater improvement in recruitment of transversus abdominis (7.8%) than participants receiving general exercise (4.9% reduction) or spinal manipulative therapy (3.7% reduction). The effect of motor control exercise on pain reduction was greater in participants who had a poor ability to recruit transversus abdominis at baseline. There was a significant, moderate correlation between improved recruitment of transversus abdominis and a reduction in disability (r = -0.35; 95% CI 0.02 to 0.62). CONCLUSION: These data provide some support for the hypothesised mechanism of action of motor control exercise and suggest that the treatment may be more effective in those with a poor ability to recruit transversus abdominis.
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Músculos Abdominales/fisiopatología , Personas con Discapacidad , Dolor de la Región Lumbar/fisiopatología , Músculos Abdominales/ultraestructura , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Enfermedad Crónica , Terapia por Ejercicio , Femenino , Humanos , Dolor de la Región Lumbar/diagnóstico por imagen , Dolor de la Región Lumbar/terapia , Masculino , Persona de Mediana Edad , Reclutamiento Neurofisiológico/fisiología , Ultrasonografía , Adulto JovenRESUMEN
PURPOSE: The constant activity of the extraocular muscles is supported by abundant mitochondria. These organelles may enhance energy production by increasing the content of respiratory complexes. The authors tested the hypothesis that extraocular muscle mitochondria respire faster than do mitochondria from limb muscles because of the higher content of respiratory complexes. METHODS: Inner mitochondrial membrane density was determined by stereological analysis of triceps surae (a limb muscle) and extraocular muscles of adult male Sprague-Dawley rats. The authors measured respiration rates of isolated mitochondria using a Clark-type electrode. The activity of respiratory complexes I, II, and IV was determined by spectrophotometry. The content of respiratory complexes was estimated by Western blot. RESULTS: States 3, 4, and 5 respiration rates in extraocular muscle mitochondria were 40% to 60% lower than in limb muscle mitochondria. Extraocular muscle inner mitochondrial membrane density was similar to that of other skeletal muscles. Activity of complexes I and IV was lower in extraocular muscle mitochondria (approximately 50% the activity in triceps), but their content was approximately 15% to 30% higher. There was no difference in complex II content or activity or complex III content. Finally, complex V was less abundant in extraocular muscle mitochondria. CONCLUSIONS: The results demonstrate that extraocular muscle mitochondria respire at slower rates than mitochondria from limb muscles, despite similar mitochondrial ultrastructure. Instead, differences were found in the activity (I, IV) and content (I, IV, V) of electron transport chain complexes. The discrepancy between activity and content of some complexes is suggestive of alternative subunit isoform expression in the extraocular muscles compared with limb muscles.
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Mitocondrias Musculares/metabolismo , Músculos Oculomotores/metabolismo , Consumo de Oxígeno/fisiología , Músculos Abdominales/metabolismo , Músculos Abdominales/ultraestructura , Animales , Western Blotting , Transporte de Electrón/fisiología , Complejo I de Transporte de Electrón/metabolismo , Complejo II de Transporte de Electrones/metabolismo , Complejo III de Transporte de Electrones/metabolismo , Complejo IV de Transporte de Electrones/metabolismo , Metabolismo Energético , Masculino , Potencial de la Membrana Mitocondrial , Membranas Mitocondriales/metabolismo , Músculos Oculomotores/ultraestructura , Ratas , Ratas Sprague-DawleyRESUMEN
Precise knowledge of muscle architecture and innervation patterns is essential for the development of accurate clinical and biomechanical models. Although the gross anatomy of the human abdominal muscles has been investigated, the finer details of their microanatomy are not well described. Fascicles were systematically sampled from each of the human abdominal muscles, and small fiber bundles from selected fascicles stained with acetylcholinesterase to determine the location of motor endplate bands, myomyonal junctions, and myotendinous junctions. Statistical analysis was used to ascertain the association between fascicular length and number of endplate bands. The number of endplate bands along a fascicle was variable between different portions of each muscle, but was strongly correlated with fascicular length (r = 0.814). In fascicles less than 50 millimeters (mm) in length, only a single endplate band was generally present, while multiple endplate bands (usually two or three) were found in fascicles longer than 50 mm. The presence of myomyonal junctions throughout the longer (>50 mm) fascicles verified that they were composed of short, intrafascicularly terminating fibers, while shorter fascicles comprised fibers spanning the entire fascicular length. This preliminary study provides evidence that multiple endplate bands are contained in some regions of the abdominal muscles, an arrangement that differs from most human appendicular muscles. It is not clear whether the variations in the described fine architectural features reflect regional differences in muscle function.
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Músculos Abdominales/ultraestructura , Anciano , Humanos , MasculinoRESUMEN
Fibulins are a family of extracellular glycoproteins associated with basement membranes and elastic fibers in vertebrates. Conservation of the fibulin-1 gene throughout metazoan evolution includes fibulin-1C and fibulin-1D alternate splice variants, although little is known about variant specific functions that would justify this striking structural conservation. We have therefore investigated the structure, localization and loss-of-function phenotype specific to both fibulin-1 variants in C. elegans. We find that fibulin-1C has specific roles during pharynx, intestine, gonad and muscle morphogenesis, being required to regulate cell shape and adhesion, whereas fibulin-1D assembles in flexible polymers that connect the pharynx and body-wall-muscle basement membranes. The assembly of fibulin-1C and fibulin-1D in multiple locations is dependent upon the presence of hemicentin, a recently described extracellular member of the immunoglobulin superfamily. We suggest that the distinct developmental roles and hemicentin-dependent assembly for fibulin-1 splice variants demonstrated here may be relevant to fibulin-1 and possibly other fibulin family members in non-nematode species.
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Proteínas de Caenorhabditis elegans/fisiología , Caenorhabditis elegans/metabolismo , Proteínas de Unión al Calcio/fisiología , Proteínas de la Membrana/fisiología , Músculos Abdominales/crecimiento & desarrollo , Músculos Abdominales/metabolismo , Músculos Abdominales/ultraestructura , Empalme Alternativo , Animales , Membrana Basal/metabolismo , Caenorhabditis elegans/genética , Caenorhabditis elegans/crecimiento & desarrollo , Proteínas de Caenorhabditis elegans/genética , Proteínas de Unión al Calcio/genética , Adhesión Celular/fisiología , Forma de la Célula/fisiología , Gónadas/crecimiento & desarrollo , Gónadas/metabolismo , Gónadas/ultraestructura , Mucosa Intestinal/metabolismo , Intestinos/crecimiento & desarrollo , Intestinos/ultraestructura , Proteínas de la Membrana/genética , Microscopía Electrónica de Transmisión , Morfogénesis , Mutación , Faringe/crecimiento & desarrollo , Faringe/metabolismo , Faringe/ultraestructuraRESUMEN
Donor nerves of different origins, when transplanted onto a previously denervated adult crayfish abdominal superficial flexor muscle (SFM), regenerate excitatory synaptic connections. Here we report that an inhibitory axon in these nerves also regenerates synaptic connections based on observation of nerve terminals with irregular to elliptically shaped synaptic vesicles characteristic of the inhibitory axon in aldehyde fixed tissue. Inhibitory terminals were found at reinnervated sites in all 12 allotransplanted-SFMs, underscoring the fact that the inhibitory axon regenerates just as reliably as the excitatory axons. At sites with degenerating nerve terminals and at sparsely reinnervated sites, we observe densely stained membranes, reminiscent of postsynaptic membranes, but occurring as paired, opposing membranes, extending between extracellular channels of the subsynaptic reticulum. These structures are not found at richly innervated sites in allotransplanted SFMs, in control SFMs, or at several other crustacean muscles. Although their identity is unknown, they are likely to be remnant postsynaptic membranes that become paired with collapse of degenerated nerve terminals of excitatory and inhibitory axons. Because these two axons have uniquely different receptor channels and intramembrane structure, their remnant postsynaptic membranes may therefore attract regenerating nerve terminals to form synaptic contacts selectively by excitatory or inhibitory axons, resulting in postsynaptic specification.
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Músculos Abdominales/inervación , Músculos Abdominales/fisiología , Neuronas Motoras/trasplante , Regeneración Nerviosa/fisiología , Unión Neuromuscular/fisiología , Músculos Abdominales/ultraestructura , Animales , Astacoidea , Microscopía Electrónica , Neuronas Motoras/fisiología , Neuronas Motoras/ultraestructura , Unión Neuromuscular/ultraestructuraRESUMEN
The time- and orientation-dependence of metabolite diffusion in giant muscle fibers of the lobster Panulirus argus was examined using (31)P- and (1)H-pulsed-field gradient nuclear magnetic resonance. The (31)P resonance for arginine phosphate and the (1)H resonances for betaine, arginine/arginine phosphate and -CH(2)/-CH groups were suitable for measurement of the apparent diffusion coefficient, D. Diffusion was measured axially, D(//), and radially, D( perpendicular ), in fibers over diffusion times of 20 to 300 ms. Diffusion was strongly anisotropic, and D(//) was higher than D( perpendicular ) at all times. Radial diffusion decreased with time until a steady-state value was reached at a diffusion time of approximately 100 ms. Changes in D( perpendicular ) occurred over a time scale that was consistent with previous measurements from fish and mammalian muscle, indicating that diffusion is hindered by the same types of barriers in these diverse muscle types. The time dependence indicated that the sarcoplasmic reticulum is the principal intracellular structure that inhibits mobility in an orientation-dependent manner in skeletal muscle. The abdominal muscles in P. argus are used for anaerobic, burst contractions during an escape maneuver. The fact that these muscle fibers have diameters that may exceed hundreds of microns in diameter, and nearly all of the mitochondria are localized near the sarcolemmal membrane, suggests that barriers that hinder radial diffusion of ATP equivalents may ultimately limit the rate of post-contractile recovery.
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Arginina/análogos & derivados , Fibras Musculares Esqueléticas/metabolismo , Palinuridae/metabolismo , Músculos Abdominales/fisiología , Músculos Abdominales/ultraestructura , Adenosina Trifosfato/metabolismo , Animales , Arginina/metabolismo , Betaína/metabolismo , Difusión , Espectroscopía de Resonancia Magnética , Fibras Musculares Esqueléticas/ultraestructura , Compuestos Organofosforados/metabolismo , Retículo Sarcoplasmático/metabolismoRESUMEN
Somatic cell nuclei of giant pandas can dedifferentiate in enucleated rabbit ooplasm, and the reconstructed eggs can develop to blastocysts. In order to observe whether these interspecies cloned embryos can implant in the uterus of an animal other than the panda, we transferred approximately 2300 panda-rabbit cloned embryos into 100 synchronized rabbit recipients, and none became pregnant. In another approach, we cotransferred both panda-rabbit and cat-rabbit interspecies cloned embryos into the oviducts of 21 cat recipients. Fourteen recipients exhibited estrus within 35 days; five recipients exhibited estrus 43-48 days after embryo transfer; and the other two recipients died of pneumonia, one of which was found to be pregnant with six early fetuses when an autopsy was performed. Microsatellite DNA analysis of these early fetuses confirmed that two were from giant panda-rabbit cloned embryos. The results demonstrated that panda-rabbit cloned embryos can implant in the uterus of a third species, the domestic cat. By using mitochondrial-specific probes of panda and rabbit, we found that mitochondria from both panda somatic cells and rabbit ooplasm coexisted in early blastocysts, but mitochondria from rabbit ooplasm decreased, and those from panda donor cells dominated in early fetuses after implantation. Our results reveal that mitochondria from donor cells may substitute those from recipient oocytes in postimplanted, interspecies cloned embryos.
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Clonación de Organismos/métodos , Implantación del Embrión/fisiología , Mitocondrias/metabolismo , Ursidae/fisiología , Músculos Abdominales/citología , Músculos Abdominales/ultraestructura , Animales , Secuencia de Bases , Gatos , Núcleo Celular/ultraestructura , Células Cultivadas , ADN Mitocondrial/metabolismo , Transferencia de Embrión , Desarrollo Embrionario y Fetal/fisiología , Femenino , Repeticiones de Microsatélite , Datos de Secuencia Molecular , Embarazo , ConejosRESUMEN
During respiration, abdominal muscles experience loads, not only in the muscle-fiber direction but also transverse to the fibers. We wondered whether the abdominal muscles exhibit a fiber architecture that is similar to the diaphragm muscle, and, therefore, we chose two adjacent muscles: the internal oblique (IO), with about the same muscle length as the diaphragm, and the transverse abdominis (TA), which is twice as long as the diaphragm. First, we used acetylcholinesterase staining to examine the distribution of neuromuscular junctions on both surfaces of the TA and IO muscles in six dogs. A maximum of four irregular bands of neuromuscular junctions crossed the IO, and as many as six bands crossed the TA, which is consistent with a discontinuous fiber architecture. In six additional dogs, we examined fiber architecture of these muscles by microdissecting 103 fascicles from the IO and 139 from the TA. Each fascicle contained between 20 and 30 muscle fibers. The mean length of nonspanning fibers (NSF) ranged from 2.8 +/- 0.3 cm in the IO to 4.3 +/- 0.5 cm in the TA, and the mean length of spanning fibers ranged from 4.3 +/- 0.5 cm in the IO to 7.6 +/- 1.4 cm in the TA. NSF accounted for 89.6 +/- 1.5% of all fibers dissected from the IO and 99.1 +/- 0.2% of all fibers dissected from the TA. The percentage of NSF with both ends tapered was 6.2 +/- 1.0 and 41.0 +/- 2.3% for IO and TA, respectively. These data show that fiber architecture in either IO or TA is discontinuous, with much more short-tapered fibers in the TA than in the IO. When abdominal muscles are submaximally activated, as during both normal expiration and maximal expiratory efforts, muscle force could be transmitted to the cell membrane and to the extracellular intramuscular connective tissue by shear linkage, presumably via structural transmembrane proteins.
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Músculos Abdominales/ultraestructura , Fibras Musculares Esqueléticas/ultraestructura , Músculos Abdominales/enzimología , Acetilcolinesterasa/metabolismo , Animales , Disección , Perros , Histocitoquímica , Unión Neuromuscular/enzimología , Coloración y Etiquetado , Distribución TisularRESUMEN
Type IIB fast fibres are typically demonstrated in human skeletal muscle by histochemical staining for the ATPase activity of myosin heavy-chain (MyHC) isoforms. However, the monoclonal antibody specific for the mammalian IIB isoform does not detect MyHC IIB protein in man and MyHC IIX RNA is found in histochemically identified IIB fibres, suggesting that the IIB protein isoform may not be present in man; if this is not so, jaw-closing muscles, which express a diversity of isoforms, are likely candidates for their presence. ATPase histochemistry, immunohistochemistry polyacrylamide gel electrophoresis and in situ hybridization, which included a MyHC IIB-specific mRNA riboprobe, were used to compare the composition and RNA expression of MyHC isoforms in a human jaw-closing muscle, the masseter, an upper limb muscle, the triceps, an abdominal muscle, the external oblique, and a lower limb muscle, the gastrocnemius. The external oblique contained a mixture of histochemically defined type I, IIA and IIB fibres distributed in a mosaic pattern, while the triceps and gastrocnemius contained only type I and IIA fibres. Typical of limb muscle fibres, the MyHC I-specific mRNA probes hybridized with histochemically defined type I fibres, the IIA-specific probes with type IIA fibres and the IIX-specific probes with type IIB fibres. The MyHC IIB mRNA probe hybridized only with a few histochemically defined type I fibres in the sample from the external oblique; in addition to this IIB message, these fibres also expressed RNAs for MyHC I, IIA and IIX. MyHC IIB RNA was abundantly expressed in histochemical and immunohistochemical type IIA fibres of the masseter, together with transcripts for IIA and in some cases IIX. No MyHC IIB protein was detected in fibres and extracts of either the external oblique or masseter by immunohistochemistry, immunoblotting and electrophoresis. Thus, IIB RNA, but not protein, was found in the fibres of two different human skeletal muscles. It is believed this is the first report of the substantial expression of IIB mRNA in man as demonstrated in a subset of masseter fibres, but rarely in limb muscle, and in only a few fibres of the external oblique. These findings provide further evidence for the complexity of myosin gene expression, especially in jaw-closing muscles.
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Músculo Masetero/metabolismo , Fibras Musculares Esqueléticas/metabolismo , Cadenas Pesadas de Miosina/análisis , Miosina Tipo IIB no Muscular/análisis , Músculos Abdominales/metabolismo , Músculos Abdominales/ultraestructura , Adenosina Trifosfatasas , Adolescente , Adulto , Electroforesis en Gel de Poliacrilamida , Femenino , Expresión Génica , Humanos , Immunoblotting , Hibridación in Situ , Masculino , Músculo Masetero/ultraestructura , Persona de Mediana Edad , Fibras Musculares de Contracción Rápida/metabolismo , Fibras Musculares de Contracción Rápida/ultraestructura , Fibras Musculares Esqueléticas/ultraestructura , Fibras Musculares de Contracción Lenta/metabolismo , Fibras Musculares de Contracción Lenta/ultraestructura , Músculo Esquelético/metabolismo , Músculo Esquelético/ultraestructura , Cadenas Pesadas de Miosina/genética , Miosina Tipo I/análisis , Miosina Tipo I/genética , Miosina Tipo II/análisis , Miosina Tipo II/genética , Miosina Tipo IIA no Muscular/análisis , Miosina Tipo IIA no Muscular/genética , Miosina Tipo IIB no Muscular/genética , Fenotipo , Isoformas de Proteínas/análisis , Isoformas de Proteínas/genética , Sondas ARN , ARN Mensajero/análisis , ARN Mensajero/genéticaRESUMEN
BACKGROUND/PURPOSE: The cremaster muscles (CM) associated with undescended testis reveal neurogenic alterations that mainly affect type 2 fibers. The ultrastructure of CM has been evaluated to define if further evidence to explain the alterations could be identified. METHODS: CM of 8 boys with inguinal hernia and 8 boys with undescended testis at similar ages were biopsied. Samples were processed for electron microscopic evaluations. Semithin and thin sections were examined under an electron microscope. RESULTS: The CM associated with inguinal hernia showed normal ultrastructure. However, some alterations were encountered in CM associated with undescended testis. Unmyelinated fibers were diminished in number, and myelinated fibers were outnumbering the unmyelinated fibers. Marked disorientation of myofibers, redundant sarcolemma, empty sleeves of basal lamina, disarray of myofibrils, densely packed myofilaments, Z disk streaming, dilated sarcoplasmic reticulum, and dense-irregularly shaped mitochondria were repeatedly encountered. Satellite cells appeared inactive. Most of the fibers were contracted. CONCLUSIONS: The decrease in number of unmyelinated fibers appears to represent a decrease in autonomic nerve fibers. The alterations within muscle fibers may reflect a deficiency in autonomic innervation. Autonomic nervous system is highly responsive to circulating androgens. Factors decreasing the vulnerability of autonomic nervous system against androgenic effects may result in a CM with neurogenic alterations, thus inhibiting testicular descent. J Pediatr Surg 36:573-578.
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Músculos Abdominales/inervación , Músculos Abdominales/ultraestructura , Sistema Nervioso Autónomo/ultraestructura , Criptorquidismo/patología , Hernia Inguinal/patología , Andrógenos/biosíntesis , Biopsia con Aguja , Preescolar , Técnicas de Cultivo , Humanos , Lactante , Masculino , Microscopía Electrónica , Fibras Musculares Esqueléticas/ultraestructura , Fibras Nerviosas/ultraestructura , Estudios Prospectivos , Sensibilidad y EspecificidadRESUMEN
BACKGROUND: After the description of a general scheme of the architecture of collagen fibers in linea alba and rectus sheaths, variability and differences of fiber architectures were analyzed to describe their functional role. MATERIALS AND METHODS: Using confocal laser scanning microscopy the diameter of each layer of fibril bundles was measured in linea alba and rectus sheaths of 12 human cadavers, and each fibril bundle was classified according to its orientation (oblique I and II, transverse). RESULTS: The mean diameter of fibril bundles in the supraumbilical region of the linea alba was smaller than in the infraumbilical region, and in the supraumbilical region the thickness of the linea alba was smaller than in the infraumbilical region. Analyzing sex-dependent differences in the fiber architecture of the linea alba, a larger amount of transverse fibers relative to oblique fibers were found in females in infraumbilical regions. The thickness of the infraumbilical linea alba was smaller in females than in males, while its width was larger. CONCLUSIONS: There exist gender differences in the architecture of the linea alba. However, whether these morphological differences demonstrate the adaptability of this fiber architecture to biomechanical stress in raised intraabdominal pressure in pregnancy remains to be proven. The transverse fibers act as a counterpart to the intraabdominal pressure whereas the oblique fibers are involved mainly in movements of the trunk.
Asunto(s)
Músculos Abdominales/ultraestructura , Colágeno/ultraestructura , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Procesamiento de Imagen Asistido por Computador , Imagenología Tridimensional , Masculino , Microscopía Confocal , Caracteres SexualesRESUMEN
Critical illness is associated with muscle wasting and muscle weakness. Using burn injury as a model of local and systemic inflammatory response, we tested the hypothesis that thermal injury causes apoptosis in muscle. After a 40% body surface area burn to rats, abdominal muscles beneath the burn and limb muscles distant from the burn were examined for apoptosis at varying times after burn. Ladder assay, ELISA, and histological methods showed evidence of apoptosis in the abdominal muscles within 4-12 h with peak changes occurring at 3-7 days. Maximal apoptosis was also evident at distant limb muscles at 3-7 days. Investigation of proapoptotic pathways indicated mitochondrial membrane potential to be altered by 1 h after burn. Starting at 15 min after burn, cytochrome c was released from the mitochondria into the cytosol, followed by increased activity of caspase-3, starting at 6 h after burn. These studies suggest that mitochondria and caspase-mediated apoptotic pathways may be an additional mechanism of muscle weight loss in burns and may be potential therapeutic targets for prevention of muscle wasting.
Asunto(s)
Apoptosis , Quemaduras/patología , Músculo Esquelético/patología , Músculos Abdominales/ultraestructura , Animales , Quemaduras/complicaciones , Caspasa 3 , Caspasas/metabolismo , Grupo Citocromo c/metabolismo , ADN , Activación Enzimática , Masculino , Potenciales de la Membrana , Mitocondrias/fisiología , Músculo Esquelético/fisiopatología , Tamaño de los Órganos , Ratas , Ratas Sprague-Dawley , Síndrome Debilitante/etiologíaRESUMEN
Infection of an expanded polytetrafluoroethylene (ePTFE) prosthesis after implant is a major drawback of its use in current clinical practice. The aim of the present study was to compare the behavior of such prostheses implanted into New Zealand rabbits with that of prostheses infected after clinical implant. Experimental implants of ePTFE Soft Tissue Patch were performed to repair defects (7 x 5 cm) created in the abdominal wall of 10 rabbits. Prior to implant the prostheses were contaminated with Staphylococcus aureus. Five animals implanted with noncontaminated ePTFE prostheses served as controls. All the animals were sacrificed at 30 days after implant. For the clinical study, specimens were taken from three ePTFE implants that had been found to be infected after intervention. The clinical and experimental implant specimens were processed for light microscopy and scanning electron microscopy. Macroscopic and microscopic examination of experimental and clinical implants revealed alterations to the ePTFE structure, such as areas of fragmentation, fracture lines, and detachment of fine layers of ePTFE that harbored numerous Staphylococcus colonies. Neoformed tissue around contaminated implants was arranged more loosely, and on occasion large spaces between fibers gave rise to an "unknitted" appearance with respect to the control implants. It may be concluded that microporous ePTFE prostheses show similar behavior following experimental or clinical implant in the presence of infection. Irreversible changes to the structure of the prosthesis are produced owing to colonization of the biomaterial by microorganisms, which in most cases necessitates total replacement of the prosthesis.
Asunto(s)
Músculos Abdominales/cirugía , Politetrafluoroetileno/efectos adversos , Infecciones Relacionadas con Prótesis/patología , Infecciones Estafilocócicas/patología , Músculos Abdominales/ultraestructura , Animales , Humanos , ConejosRESUMEN
OBJECTIVE: To investigate the local tissue reactions to meshes that had been removed from humans. DESIGN: Open study SETTING: Surgical department of the technical University, Aachen, Germany. MATERIAL: Samples of 17 non-absorbable meshes (1 polyester, 10 polypropylene, 2 reduced polypropylene, and 4 polytetrafluorethylene, PTFE) and 1 absorbable mesh (polyglactin 910) that had been implanted for repair of abdominal wall defects. INTERVENTIONS: Light and transmission electron microscopy, immunohistochemistry, and histological examination. MAIN OUTCOME MEASURES: Signs of inflammatory response. RESULTS: Light microscopy showed chronic inflammatory tissue reaction, even after years, with pronounced differences among materials. Partial volume of inflammatory cells (%) varied from 32 in polypropylene, to 12 in expanded PTFE, 8 in polyester, and 7 in reduced polypropylene. Formation of connective tissue correlated significantly with the extent of the inflammatory reaction (p<0.01). In meshes implanted for long periods there were still numerous macrophages at the interface between tissue and polypropylene (45%), polyester (45%), expanded PTFE (25%), and reduced polypropylene (22%). There was no difference in time dependent tissue reactions (p = 0.19). CONCLUSION: Inflammation around alloplastic materials used to repair defects in the abdominal wall persists for many years. There was evidence of long term wound complications as a result of persistent foreign body reactions. Further studies are required to evaluate the long term tissue response to these materials.
Asunto(s)
Músculos Abdominales/cirugía , Reacción a Cuerpo Extraño/etiología , Hernia Ventral/complicaciones , Mallas Quirúrgicas/efectos adversos , Músculos Abdominales/metabolismo , Músculos Abdominales/ultraestructura , Adulto , Anciano , Femenino , Reacción a Cuerpo Extraño/metabolismo , Reacción a Cuerpo Extraño/patología , Hernia Ventral/metabolismo , Hernia Ventral/patología , Hernia Ventral/cirugía , Humanos , Inmunohistoquímica , Masculino , Microscopía Electrónica , Persona de Mediana Edad , Reoperación , Mallas Quirúrgicas/estadística & datos numéricosRESUMEN
Modern surgical hernia repair depends increasingly on synthetic meshes for the reconstruction of the abdominal wall. Despite the undisputed advantages of the polypropylene (PP) meshes currently available (Marlex, Prolene), reports of complications after implantation are increasing. Although, serious complications such as perforation and fistula formation are rare, minor and local complaints such as seromas, misfeelings and a decreased abdominal wall mobility are observed in about one-half of the patients. In regard to the exaggerated strength of the currently available mesh modifications a reduction of the material should improve the integration of the meshes into the artificial abdominal wall. In the present study, the commercially available basic mesh Prolene has been compared to two newly constructed PP-mesh modifications with reduced amounts of PP. The modifications have gradually been adopted to the physiological requirements of abdominal wall stability and mobility by reducing the amount of PP to 64% (E-BLUE) and 24% (variant A) of the Prolene mesh (developed by ETHICON, Norderstedt, Germany). All PP-mesh variants have been implanted in a rat model and studied by 3D-photogrammetry, tensiometry, light- and electron microscopy, as well as morphometry over implantation intervals of 3, 7, 14, 21 and 90 days. The data show that current constructions of PP-meshes are oversized and definitely restrict abdominal wall mobility in the present model. Sufficient stability of the artificial abdominal wall is even guaranteed by PP-mesh modifications with a reduction of PP-quantity to about 25% of the Prolene mesh. The degree of fibrosis directly correlated with abdominal wall restriction, whereas the formation of connective tissue in the interface PP-fibre/host-issue depends on the amount and activity of the inflammatory reaction. The quantity and quality of inflammation, again, directly relies to the amount of PP and to the surface area in contact with the recipient tissues. Altogether, the present study suggests that a modification of the PP-meshes could be helpful to prevent major and minor complications of surgical PP-meshes.