RESUMEN
Synovial fluid lubricates articular joints by forming a hydrated layer between the cartilage surfaces. In degenerative joint diseases like osteoarthritis (OA), the synovial fluid is compromised, which leads to less effective innate lubrication and exacerbated cartilage degeneration. Studies over the years have led to the development of partially or fully synthetic biolubricants to reduce the coefficient of friction with cartilage in knee joints. Cartilage-adhering, hydrated lubricants are particularly important to provide cartilage lubrication and chondroprotection under high normal load and slow speed. Here, we report the development of a hyaluronic acid (HA)-based lubricant functionalized with cationic branched poly-L-lysine (BPL) molecules that bind to cartilage via electrostatic interactions. We surmised that the electrostatic interactions between the BPL-modified HA molecules (HA-BPL) and the cartilage facilitate localization of the HA molecules to the cartilage surface. The number of BPL molecules on the HA backbone was varied to determine the optimal grafting density for cartilage binding and HA localization. Collectively, our results show that our HA-BPL molecules adhered readily to cartilage and were effective as a lubricant in cartilage-on-cartilage shear measurements where the modified HA molecules significantly reduce the coefficient of friction compared to phosphate-buffered saline or HA alone. This proof-of-concept study shows how the incorporation of cartilage adhering moieties, such as cationic molecules, can be used to enhance cartilage binding and lubrication properties of HA.
Asunto(s)
Cartílago Articular , Cationes , Ácido Hialurónico , Lubrificación , Polilisina , Ácido Hialurónico/química , Ácido Hialurónico/farmacología , Adsorción , Cartílago Articular/efectos de los fármacos , Cartílago Articular/metabolismo , Cationes/química , Animales , Polilisina/química , Polilisina/farmacología , Bovinos , Lubricantes/química , Lubricantes/farmacología , Fricción/efectos de los fármacos , Líquido Sinovial/metabolismo , Líquido Sinovial/química , Líquido Sinovial/efectos de los fármacosRESUMEN
Catheter-related infections are one of the most common nosocomial infections with increasing morbidity and mortality, and robust antibacterial or antifouling catheter coatings remain great challenges for long-term implantation. Herein, multifunctional hydrogel coatings were developed to provide persistent and self-adaptive antifouling and antibacterial effects with self-healing and lubricant capabilities. Polyvinyl alcohol (PVA) with ß-cyclodextrin (ß-CD) grafts (PVA-Cd) and 4-arm polyethylene glycol (PEG) with adamantane and quaternary ammonium compound (QAC) terminals (QA-PEG-Ad) were crosslinked through host-guest recognitions between adamantane and ß-CD moieties to acquire PVEQ coatings. In response to bacterial infections, QACs exhibit reversible transformation between zwitterions (pH 7.4) and cationic lactones (pH 5.5) to generate on-demand bactericidal effect. Highly hydrophilic PEG/PVA backbones and zwitterionic QACs build a lubricate surface and decrease the friction coefficient 10 times compared with that of bare catheters. The antifouling hydrated layer significantly inhibits blood protein adsorption and platelet activation and reveals negligible hemolysis and cytotoxicity. The dynamic host-guest crosslinking achieves full self-healing of cracks in PVEQ hydrogels, and the mechanical profiles were recovered to over 90 % after rejuvenating the broken hydrogels, exhibiting a long-term stability after mechanical stretching, twisting, knotting and compression. After subcutaneous implantation and local bacterial infection, the retrieved PVEQ-coated catheters display no tissue adhesion and 3 log folds lower bacterial number than that of bare catheters. PVEQ coatings effectively prevent the repeated bacterial infections and there are few inflammatory reactions in the surrounding tissue, while substantial lymphoid infiltration and inflammatory cell aggregation occur in muscle tissues around the bare catheter. Thus, this study demonstrates a catheter coating strategy by on-demand bactericidal, self-adaptive antifouling, self-healing and lubricant hydrogels to address medical devices-related infections. STATEMENT OF SIGNIFICANCE: It is estimated over two billion peripheral intravenous catheters are annually used in hospitals around the world, and catheter-associated infection has become a great clinical challenge with rapidly rising morbidity and mortality. Surface coating is considered a promising approach, but substantial challenges remain in the development of coatings that simultaneously satisfy both anti-fouling and antibacterial attributes. Even more, few attempts have been made to design mechanically robust coatings and reversible antibacterial or antifouling capabilities, which are critical for long-term medical implants. To address these challenges, we propose a concise strategy to develop hydrogel coatings from commercially available poly(ethylene glycol) and polyvinyl alcohol. In addition to self-healing and lubricant capabilities, the reversible conversion between zwitterionic and cationic lactones of quaternary ammonium compounds enables on-demand bactericidal and self-adaptive antifouling effects.
Asunto(s)
Antibacterianos , Catéteres , Materiales Biocompatibles Revestidos , Hidrogeles , Lubricantes , Hidrogeles/química , Hidrogeles/farmacología , Materiales Biocompatibles Revestidos/farmacología , Materiales Biocompatibles Revestidos/química , Animales , Antibacterianos/farmacología , Antibacterianos/química , Lubricantes/farmacología , Lubricantes/química , Alcohol Polivinílico/química , Alcohol Polivinílico/farmacología , Incrustaciones Biológicas/prevención & control , Polietilenglicoles/química , Polietilenglicoles/farmacología , Ratones , beta-Ciclodextrinas/química , HumanosRESUMEN
Development of high-performance joint injection lubricants has become the focus in the field of osteoarthritis treatment. Herein, natural product of angelica essential oil combined with the graphene oxide were prepared to the stable Pickering emulsion as a biological lubricant. The tribological properties of the Pickering emulsion under different friction conditions were studied. The lubricating mechanism was revealed and the biological activities were evaluated. Results showed that the prepared Pickering emulsion displayed superior lubrication property at the Ti6Al4V biological material interface. The maximum friction reduction and anti-wear abilities of the Pickering emulsion were improved by 36% and 50% compared to water, respectively. This was primarily due to the action of the double-layer lubrication films composed of the graphene oxide and angelica essential oil molecules. It was worth noting that the friction reduction effect of the Pickering emulsion at the natural cartilage interface was higher about 19% than that of HA used in clinic for OA commonly. In addition, the Pickering emulsion also displayed antioxidant activity and cell biocompatibility, showing a good clinical application prospect in the future.
Asunto(s)
Angelica , Emulsiones , Lubrificación , Aceites Volátiles , Aceites Volátiles/química , Aceites Volátiles/farmacología , Emulsiones/química , Angelica/química , Productos Biológicos/química , Productos Biológicos/farmacología , Grafito/química , Grafito/farmacología , Lubricantes/química , Lubricantes/farmacología , Humanos , Propiedades de Superficie , Tamaño de la Partícula , Animales , Antioxidantes/química , Antioxidantes/farmacología , Supervivencia Celular/efectos de los fármacosRESUMEN
Vaginal lubricants are commonly used to aid sexual pleasure and/or to help combat vaginal dryness and dyspareunia. Several studies have reported their impact on sperm function, however there are no published guidelines to help healthcare professionals and couples select a vaginal lubricant that is 'sperm-safe'. To address this, we conducted a literature search using both PubMed and Scopus to identify and appraise manuscripts that reported the impact of lubricants on sperm function. We did not restrict the literature search by year of publication, and we only included manuscripts that looked at the impact of vaginal lubricants on human sperm. The quality of the eligible studies was assessed using the Björndahl et al., (2016) checklist for semen analysis, as most of the studies reported the findings of a basic semen analysis. A total of 24 articles were eligible for analysis with a total of 35 vaginal lubricants (that were available to buy over the counter) being included, 2 of which studied the effect of vaginal lubricants on sperm function in vivo, and 22 being conducted in vitro. KY Jelly, PreSeed and Astroglide were most studied, with most manuscripts focussing on their impact on sperm motility. A paucity of data on most lubricants combined with methodological variations between studies and limited/no reporting on pregnancy outcomes means greater efforts are required before an evidence-based guideline can be published.
Asunto(s)
Lubricantes , Motilidad Espermática , Femenino , Humanos , Masculino , Lubricantes/farmacología , Semen , Espermatozoides , Análisis de SemenRESUMEN
Tethered-liquid perfluorocarbons (TLPs) are a class of liquid-infused surfaces with the ability to reduce blood clot formation (thrombosis) on blood-contacting medical devices. TLP comprises a tethered perfluorocarbon (TP) infused with a liquid perfluorocarbon (LP); this LP must be retained to maintain the antithrombotic properties of the layer. However, the stability of the LP layer remains in question, particularly for medical devices under blood flow. In this study, the lubricant thickness is spatially mapped and quantified in situ through confocal dual-wavelength reflection interference contrast microscopy. TLP coatings prepared on glass substrates are exposed to the flow of 37% glycerol/water mixtures (v/v) or whole blood at a shear strain rate of around 2900 s-1 to mimic physiological conditions (similar to flow conditions found in coronary arteries). Excess lubricant (>2 µm film thickness) is removed upon commencement of flow. For untreated glass, the lubricant is completely depleted after 1 min of shear flow. However, on optimized TLP surfaces, nanoscale films of lubricants (thickness between 100 nm and 2 µm) are retained over many tens of minutes of flow. The nanoscale films conform to the underlying structure of the TP layer and are sufficient to prevent the adhesion of red blood cells and platelets.
Asunto(s)
Fluorocarburos , Lubricantes , Lubricantes/farmacología , Lubricantes/química , ExcipientesRESUMEN
Purpose: To evaluate the link between the viscosity of ophthalmic formulation and tear film stability using a novel in vitro eye model. Methods: The viscosities and noninvasive tear breakup time (NIKBUT) of 13 commercial ocular lubricants were measured to evaluate the correlation between viscosity and NIKBUT. The complex viscosity of each lubricant was measured three times for each angular frequency (ranging from 0.1 to 100 rad/s) using the Discovery HR-2 hybrid rheometer. The NIKBUT measurements were performed eight times for each lubricant using an advanced eye model mounted on the OCULUS Keratograph 5M. A contact lens (CL; ACUVUE OASYS [etafilcon A]) or a collagen shield (CS) was used as the simulated corneal surface. Phosphate-buffered saline was used as a simulated fluid. Results: The results showed a positive correlation between viscosity and NIKBUT at high shear rates (at 10 rad/s, r = 0.67) but not at low shear. This correlation was even better for viscosities between 0 and 100 mPa*s (r = 0.85). Most of the lubricants tested in this study also had shear-thinning properties. OPTASE INTENSE, I-DROP PUR GEL, I DROP MGD, OASIS TEARS PLUS, and I-DROP PUR had higher viscosity in comparison to other lubricants (P < 0.05). All of the formulations had a higher NIKBUT than the control (2.7 ± 1.2 seconds for CS and 5.4 ± 0.9 seconds for CL) without any lubricant (P < 0.05). I-DROP PUR GEL, OASIS TEARS PLUS, I-DROP MGD, REFRESH OPTIVE ADVANCED, and OPTASE INTENSE had the highest NIKBUT using this eye model. Conclusions: The results show that the viscosity is correlated with NIKBUT, but further work is necessary to determine the underlying mechanisms. Translational Relevance: The viscosity of ocular lubricants can affect NIKBUT and tear film stability, so it is an important property to consider when formulating ocular lubricants.
Asunto(s)
Lentes de Contacto , Ojo , Viscosidad , Glicerol , Lubricantes/farmacologíaRESUMEN
Bacterial nanocellulose (BNC) is a naturally derived hydrogel that has recently paved its way in several biomedical applications. Despite its remarkable tissue-like properties, BNC does not express innate anticoagulant or antimicrobial properties; therefore, appropriate post-modification procedures are required to prevent nonspecific adhesion and enhance the hemocompatibility properties of BNC-based biointerface. Here, we report a new class of flexible, lubricant-infused BNC membranes with superior antithrombotic and antibacterial properties. Using chemical vapor deposition, porous BNC membranes were functionalized with fluorosilane molecules and further impregnated with a fluorocarbon-based lubricant. Compared with unmodified BNC membranes and commercially available poly(tetrafluoroethylene) (PTFE) felts, our developed lubricant-infused BNC samples significantly attenuated plasma and blood clot formation, and prevented bacterial migration, adhesion, and biofilm formation and exhibited superior fat and enzyme repellency properties. Moreover, when subjected to mechanical testing, the lubricant-infused BNC membranes demonstrated a significantly higher tensile strength and greater fatigue resistance when compared with unmodified BNC samples and PTFE felts. Overall, the superior mechanical strength and antithrombotic, antibacterial, and fat/enzyme resistant properties observed in the developed super-repellent BNC-based membranes render their application promising for various biofluid-contacting medical implants and tissue engineering constructs.
Asunto(s)
Fibrinolíticos , Lubricantes , Lubricantes/farmacología , Lubricantes/química , Bacterias , Antibacterianos/farmacología , PolitetrafluoroetilenoRESUMEN
After cardiac surgery, tissue damage to the heart may cause adhesion between heart and its surrounding tissues. Post-operative cardiac adhesion may lead to limited normal cardiac function, decreased quality of cardiac surgery, and increased risk of major bleeding during reoperation. Therefore, it is necessary to develop an effective anti-adhesion therapy to overcome cardiac adhesion. An injectable polyzwitterionic lubricant is developed to prevent adhesion between the heart and surrounding tissues and to maintain normal pumping function of the heart. This lubricant is evaluated in a rat heart adhesion model. Poly (2-methacryloyloxyethyl phosphorylcholine) (i.e., PMPC) polymers are successfully prepared via free radical polymerization of monomer MPC, and the optimal lubricating performance, biocompatibility both in vitro and in vivo is demonstrated. Besides, a rat heart adhesion model is conducted to evaluate the bio-functionality of lubricated PMPC. The results prove that PMPC is a promising lubricant for complete adhesion-prevention. The injectable polyzwitterionic lubricant shows excellent lubricating properties and biocompatibility and can effectively prevent cardiac adhesion.
Asunto(s)
Lubricantes , Metacrilatos , Lubricantes/farmacología , Polímeros , Fosforilcolina/farmacología , Propiedades de SuperficieRESUMEN
The lubrication deficiency in joints is a major cause of osteoarthritis. One of the most commonly used treatment means is to inject artificial lubricants, but there is a potential risk of infection during the injection process. Therefore, developing artificial lubricants with dual functions of friction-reduction and antibacterial is urgent. In this work, a novel polysaccharide-derived lubricant with simultaneous anti-bacteria and water-lubrication properties, called CS-MPC-N, is developed by grafting 2methacryloyloxylethyl phosphorylcholine (MPC) and nisin peptide onto backbone of chitosan (CS). Compared to the control CS, CS-MPC-N exhibits good lubrication and friction-reduction properties because of its excellent water solubility. Especially, CS-MPC-N shows low friction coefficient (0.03 â¼ 0.05) at the sliding interfaces of artificial joints materials or even natural articular cartilages. Moreover, CS-MPC-N can effectively inhibit the proliferation of Staphylococcus aureu, exhibiting excellent antibacterial effect. This kind of novel polysaccharide-derived lubricant is expected to be used in treating infectious arthritis.
Asunto(s)
Quitosano , Quitosano/farmacología , Lubrificación , Lubricantes/farmacología , Lubricantes/química , Biomimética , Antibacterianos/farmacología , Agua , FricciónRESUMEN
The lubricating properties of the lubricants were tested under boundary friction conditions; it was found that the surface-active additives had reduced the wear mark and thus the value of the Goz/40 parameter (limiting load of wear). The introduction of a surfactant containing amide compounds into the oils and greases was highly effective in slowing down the oxidation process. Lubricants containing mono-15 ([3-(N,N,N-dimethylbuthylamine)propyl]hexadecanamide chloride) and 15-4-15 (tetramethylene-bis [3-(N,N,N-dimethylamine)propyl]hexadecanamide) additives were characterised by higher oxidation stability compared to the unmodified lubricants. Both of the analysed substances showed bactericidal properties against Staphylococcus aureus and Salmonella enteritica. Tests of antibacterial activity in the lubricants with the addition of mono-15 and 15-4-15 confirmed that these lubricants can be considered bactericidal against Gram-positive and Gram-negative bacteria.
Asunto(s)
Antibacterianos , Lubricantes , Lubricantes/farmacología , Antibacterianos/farmacología , Bacterias Gramnegativas , Bacterias Grampositivas , Amidas/farmacologíaRESUMEN
Thrombus formation and infections caused by bacterial adhesion are the most common causes of failure in blood-contacting medical devices. Reducing the interaction of pathogens using repellent surfaces has proven to be a successful strategy in preventing device failure. However, designing scale-up methodologies to create large-scale repellent surfaces remains challenging. To address this need, we have created an all-polymeric lubricant-infused system using an industrially viable swelling-coagulation solvent (S-C) method. This induces hierarchically structured micro/nano features onto the surface, enabling improved lubricant infusion. Poly(3,3,3-trifluoropropylmethylsiloxane) (PTFS) was used as the lubricant of choice, a previously unexplored omniphobic nonvolatile silicone oil. This resulted in all-polymeric liquid-infused surfaces that are transparent and flexible with long-term stability. Repellent properties have been demonstrated using human whole blood and methicillin-resistant Staphylococcus aureus (MRSA) bacteria matrices, with lubricated surfaces showing 93% reduction in blood stains and 96.7% reduction in bacterial adherence. The developed material has the potential to prevent blood and pathogenic contamination for a range biomedical devices within healthcare settings.
Asunto(s)
Manchas de Sangre , Staphylococcus aureus Resistente a Meticilina , Humanos , Lubricantes/farmacologíaRESUMEN
Slippery liquid-filled porous surfaces (SLIPS) have attracted extensive research attention for their unique repellent properties, but such surfaces typically lack essential bactericidal activity and cannot defend against the spread of bacteria once bacterial contamination occurs. Herein, a slippery liquid-infused porous surface (SLIPS), endowed with both super-repellent and contact-killing antimicrobial performances is reported. Firstly, polystyrene (PS) based porous structures are developed via a facile microphase separation technique with poly(ethylene glycol) (PEG) as the sacrifice template. The porous surface was then covalently modified by 3-(trimethoxysilyl)propyl dimethyl undecyl ammonium chloride (QAC-Silane) to get the contact-killing antimicrobial performances. After lubricant (silicone oil) is introduced to the porous structure, the SLIPS surface demonstrates remarkably high super-repellence against both Gram-positive and negative bacteria, and also maintains essential contact-killing antimicrobial activities from the fixed QAC-11 groups, once the infused lubricant was depleted. Also, this surface demonstrates a reduced coefficient of friction (COF) of â¼56% as compared to that of the control flat surface. Moreover, this SLIPS surface can be easily realized on various substrates, such as silica glass, polycarbonate (PC), polyethylene terephthalate (PET), polyethylene (PE) and silicone catheter tube. Owing to its simple, low-cost and fast fabrication approach, this kind of surface may find unique biomedical applications where an effective antibacterial performance and lubricity are highly needed.
Asunto(s)
Antibacterianos , Lubricantes , Porosidad , Propiedades de Superficie , Lubricantes/farmacología , Lubricantes/química , Antibacterianos/farmacología , Fricción , BacteriasRESUMEN
OBJECTIVE: To assess the effects of "sperm-friendly" coital lubricants on sperm motility. METHODS: This study compared the effects of five lubricants (Optilube®, Pre-Seed®, Yes Baby®, olive oil, and egg white) on sperm motility in 60 normozoospermic semen samples obtained from men attending a private fertility clinic. Samples were exposed to each of the lubricants, with untreated samples serving as controls, and were examined microscopically at four defined time-points from 2 to 72 h after liquefaction. Sperm motility was graded according to World Health Organization criteria. RESULTS: With the exception of egg white, all lubricants caused significant (P < 0.001) reductions in sperm forward progression compared with untreated controls until 24 h after liquefaction. Furthermore, between-group comparisons of the commercially available lubricants revealed statistically significant differences in forward progression motility: Pre-Seed® was superior to Optilube® (P < 0.001), which in turn was superior to Yes Baby® (P < 0.001) at 2-4 h after exposure. Significance (P < 0.001) between Pre-Seed® and Yes Baby® was maintained until 24 h. CONCLUSION: Although spermatozoa exposed to Pre-Seed® demonstrated greater motility than spermatozoa exposed to Yes Baby®, claims that these lubricants are sperm-friendly were refuted. Conversely, egg white was shown to be a sperm-friendly lubricant for couples who are trying to conceive.
Asunto(s)
Lubricantes , Motilidad Espermática , Celulosa/análogos & derivados , Humanos , Lubricantes/farmacología , Masculino , Poloxámero , Semen , EspermatozoidesRESUMEN
Poly(3-hydroxybutyrate) (PHB) is a promising substitute to petroleum-based polymers in packaging and biomedical applications provided that its melt processability and degradability are improved. A new method to control the properties of PHB by using cheap calcium stearate (CS) as a lubricant and decomposition catalyst in melt-mixed PHB-CS compounds was first used. CS is composed of a metallic cation, which promotes PHB degradation, and a hydrophobic anion that improves the compatibility with PHB and processability. An environmentally friendly melt mixing technique was employed to obtain the PHB-CS compounds. Incorporation of 0.5 or 5 wt% CS reduced the melt viscosity and molecular weight of PHB, decreased the melting temperature with up to 5 °C, the crystallization temperature with more than 25 °C, and the degradation temperature with 15 and 40 °C, respectively. In small amounts (0.05 wt%), CS improved the processability and mechanical properties of PHB. In higher amount (0.5 wt%), CS slightly improved the Young's modulus, reduced the tensile strength and enhanced degradation. A better control of thermal and mechanical properties of PHB is, thus, possible by using different CS amount and processing conditions. These results are relevant for PHB application in the context of the global transition to biodegradable packaging.
Asunto(s)
Hidroxibutiratos/química , Lubricantes/farmacología , Poliésteres/química , Ácidos Esteáricos/farmacología , Temperatura , Rastreo Diferencial de Calorimetría , Catálisis , Cristalización , Módulo de Elasticidad , Microscopía de Fuerza Atómica , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie , Resistencia a la Tracción , Termogravimetría , Factores de Tiempo , TorqueRESUMEN
Prolonged use of tight-fitting PPE, e.g., by COVID-19 healthcare workers leads to skin injuries. An important contributor is the shear exerted on the skin due to static friction at the skin-PPE interface. This study aims to develop an optimised wax-oil lubricant that reduces the friction, or shear, in the skin-PPE contact for up to four hours. Lubricants with different wax-oil combinations were prepared using beeswax, paraffin wax, olive oil, and mineral oil. In-vivo friction measurements involving seven participants were conducted by sliding a polydimethylsiloxane ball against the volar forearms to simulate the skin-PPE interface. The maximum static coefficient of friction was measured immediately and four hours after lubricant application. It was found that the coefficient of friction of wax-oil lubricants is mainly governed by the ratio of wax to oil and the thermal stability and morphology of the wax. To maintain long-term lubricity, it is crucial to consider the absorption of oil into the PPE material. The best performing lubricant is a mixture of 20 wt% beeswax, 40 wt% olive oil, and 40 wt% mineral oil, which compared to unlubricated skin, provides 87% (P = 0.0006) and 59% (P = 0.0015) reduction in instantaneous and 4-h coefficient of friction, respectively.
Asunto(s)
Lubricantes/química , Lubricantes/farmacología , Equipo de Protección Personal/efectos adversos , Piel/efectos de los fármacos , Ceras , Adulto , COVID-19 , Femenino , Antebrazo/fisiología , Humanos , Masculino , Aceite Mineral/química , Aceite de Oliva/química , Factores de Tiempo , Ceras/químicaRESUMEN
The outer layers of the vaginal epithelium (VE) are important because they accumulate glycogen which, under optimal conditions, Lactobacillus spp. consume to grow and acidify the vaginal microenvironment with lactic acid. We hypothesized that exposure to lubricant, for example in the conduct of a transvaginal ultrasound (TVUS), may contribute to the shedding of mature epithelial cells, exposing immature cells. Cervicovaginal fluid (CVF) was sampled at four time points by menstrual cup (Softdisc™) from 50 women referred for TVUS, during which a controlled volume of lubricant was applied to the TVUS wand. Samples were collected (1) immediately before TVUS and (2) 6-12 hours, (3) within one week, and (4) two weeks after TVUS. Clinical vaginal lubricants are similar to commercial lubricants, and often have a high osmolality or pH, and contain bactericides such as methylparaben and propylparaben. The number and maturity of epithelial cells in each CVF sample were measured by quantitative and differential fluorimetry (maturity index, MI). Comparisons of cell-counts and maturity were made by paired Wilcoxon signed-rank tests. Among women with a high pre-TVUS MI (> 3), there was a decrease in median cell-count and mean MI in the sample collected 6-12 hours after TVUS (p<0.001, n = 26 and p < 0.001, n = 26, respectively). For these women, cell-count and MI remained lower in the sample collected within the subsequent week (p<0.001, n = 29 and p<0.01, n = 29, respectively), and MI remained lower in the sample collected within two weeks of TVUS (p<0.01, n = 25), compared to the pre-TVUS sample. Among participants with a low pre-TVUS MI (< 3), cell-count was higher in the sample collected within two weeks of TVUS compared to the pre-TVUS sample (p = 0.03, n = 15), but no significant changes in MI were observed. Results were similar when restricted to reproductive-age women. This preliminary data indicates hypertonic vaginal lubricants may increase vaginal epithelial cell shedding.
Asunto(s)
Endosonografía/métodos , Células Epiteliales/efectos de los fármacos , Lubricantes/farmacología , Vagina/efectos de los fármacos , Adulto , Femenino , Humanos , Lubricantes/administración & dosificación , Lubricantes/efectos adversos , Lubrificación/métodos , Persona de Mediana Edad , Concentración Osmolar , Vagina/citologíaRESUMEN
The application of stannous ions in combination with fluoride (F/Sn) is one of the central strategies in reducing erosive tooth wear. F/Sn efficacy can be enhanced by adding chitosan, a positively charged biopolymer. For patients with low saliva flow, this efficacy, however, is not sufficient, making further improvement desirable. This could be achieved by combining chitosan with other molecules like mucin, which together might form multilayers. This in-vitro study aimed to investigate the effect of chitosan, mucin, F/Sn and combinations thereof on enamel erosion and erosion-abrasion. Human enamel samples (n = 448, 28 groups) were cyclically eroded or eroded-abraded (10 days; 6 × 2 min erosion and 2 × 15 s/200 g abrasion per day). Samples were treated 2 × 2 min/day with solutions containing either, chitosan (50 or 500 mPas), porcine gastric mucin, F/Sn or combinations thereof after abrasive challenge. Tissue loss was measured profilometrically, interaction between hard tissue and active agents was assessed with energy dispersive spectroscopy and scanning electron microscopy. Chitosan and F/Sn showed the expected effect in reducing tissue loss under erosive and under erosive-abrasive conditions. Neither mucin alone nor the combinations with mucin showed any additional beneficial effect.
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Quitosano/farmacología , Esmalte Dental/efectos de los fármacos , Lubricantes/farmacología , Mucinas/farmacología , Fluoruros de Estaño/farmacología , Abrasión de los Dientes/prevención & control , Erosión de los Dientes/prevención & control , Esmalte Dental/diagnóstico por imagen , Humanos , Microscopía Electrónica de Rastreo/métodos , Espectrometría por Rayos X/métodos , Abrasión de los Dientes/diagnóstico por imagen , Erosión de los Dientes/diagnóstico por imagen , Cepillado Dental , Pastas de Dientes/químicaRESUMEN
This study aims to explore the production and physicochemical properties of an exopolysaccharide (EPS) produced from soil isolate, Paenibacillus sp. ZX1905 in submerged culture. The highest EPS production of 15.67 g/L was achieved in a medium containing soluble starch, peptone and inorganic salts. The purified EPS exhibited excellent skin lubricating properties and was named lubcan. The chemical analysis reviewed that lubcan was an acidic heteropolysaccharide consisted of glucuronic acid, glucose, mannose, galactose, and rhamnose in a molar ratio of 2:3:1:2:2, and the average molecular mass was about 3.27 × 106 Da. NMR and methylation analysis revealed that lubcan backbone was composed of 1,4-α-Man, 1,4,6-α-Glc, 1,3-α-Gal, 1,3-ß-Rha, and 1,3-ß-Gal, together with the branches of 1,3-α-Glc, 1,3-α-Rha, two 1,4-α-GlcA, and terminal-α-Glc(4,6-pyr). The lubcan solution exhibited stability at pH ranging from 5.0 to 7.0, temperature between 5 and 50 °C, and monovalent salts (0.2 M) and divalent salts (0.05 M) addition. The moisture absorption rates of lubcan were 16.98% and 40.41% under the conditions of 43% and 81% relative humidity, which were close to that of hyaluronic acid (17.28% and 41.20%, respectively). These properties make lubcan a good alternative to more expensive hyaluronic acid in the cosmetic industry.
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Lubricantes/química , Lubricantes/farmacología , Paenibacillus/crecimiento & desarrollo , Polisacáridos Bacterianos/química , Polisacáridos Bacterianos/farmacología , Animales , Secuencia de Carbohidratos , Medios de Cultivo , Estabilidad de Medicamentos , Concentración de Iones de Hidrógeno , Ratones , Peso Molecular , Paenibacillus/química , Paenibacillus/aislamiento & purificación , Reología , Microbiología del SueloRESUMEN
The mechanical component in the pathophysiology of dry eye disease (DED) deserves attention as an important factor. The lubrication deficit induced impaired mechano-transduction of lid pressure to the ocular surfaces may lead to the dysregulation of homeostasis in the epithelium, with sensations of pain and secondary inflammation. Ocular pain is possibly the first sign of attrition and may occur in the absence of visible epithelial damage. Attrition is a process which involves the constant or repeated challenge of ocular surface tissues by mechanical shear forces; it is enhanced by the thinning of corneal epithelium in severe DED. As a highly dynamic process leading to pain and neurogenic inflammation, the identification of the impact of attrition and its potential pathogenic role could add a new perspective to the current more tear film-oriented models of ocular surface disease. Treatment of DED addressing lubrication deficiencies and inflammation should also consider the decrease of attrition in order to stimulate epithelial recovery and neural regeneration. The importance of hyaluronic acid, its molecular characteristics, the extracellular matrix and autoregulative mechanisms in this process is outlined. The identification of the attrition and recognition of its impact in dry eye pathophysiology could contribute to a better understanding of the disease and optimized treatment regimens.
Asunto(s)
Síndromes de Ojo Seco/patología , Epitelio Corneal/patología , Antiinflamatorios/farmacología , Antiinflamatorios/uso terapéutico , Síndromes de Ojo Seco/tratamiento farmacológico , Síndromes de Ojo Seco/metabolismo , Epitelio Corneal/efectos de los fármacos , Epitelio Corneal/metabolismo , Matriz Extracelular/metabolismo , Homeostasis , Humanos , Lubricantes/farmacología , Lubricantes/uso terapéutico , Mecanotransducción Celular/efectos de los fármacosRESUMEN
Half of postmenopausal women experience genitourinary syndrome of menopause, for which many use lubricating vaginal products. The effect of vaginal products on uropathogenic and commensal vaginal bacteria is poorly understood. We evaluated the effect of five common vaginal products (KY Jelly, Replens Silky Smooth lubricant, coconut oil, Replens Long-Lasting moisturizer or Trimo-San) on growth and viability of Escherichia coli and Lactobacillus crispatus. Bacteria were co-cultured products alone and in the presence of both vaginal epithelial cells and selected products. Bacterial growth was compared between conditions using an unpaired t-test or ANOVA, as appropriate. All products except for coconut oil significantly inhibited growth of laboratory and clinical strains of Escherichia coli (p < 0.02). Only two products (Replens Long-Lasting moisturizer and Trimo-San) significantly inhibited growth of Lactobacillus crispatus (p < 0.01), while the product Replens Silky Smooth stimulated growth (p < 0.01). Co-culture of selected products in the presence of vaginal epithelial cells eliminated the inhibitory effects of the products on E. coli. In conclusion, in vitro exposure to vaginal moisturizing and lubricating products inhibited growth of Escherichia coli, though the inhibition was mitigated by the presence of vaginal epithelial cells. Lactobacillus crispatus demonstrated less growth inhibition than Escherichia coli.