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1.
Rev. argent. microbiol ; Rev. argent. microbiol;46(3): 201-204, oct. 2014.
Artículo en Inglés | LILACS | ID: biblio-1009975

RESUMEN

La leptospirosis es una enfermedad infecciosa de amplia distribución global; endémica en Argentina. El objetivo de este estudio fue obtener los perfiles genéticos de las cepas de Leptospira spp. aisladas de casos clínicos de perros provenientes de la provincia de Buenos Aires, empleando el análisis de repeticiones en tándem de número variable en múltiples locus [multiple-locus variable-number tandem repeats analysis (MLVA)]. Fueron genotipificadas por MLVA ocho cepas aisladas de perros. Se obtuvo un perfil idéntico al de Leptospira interrogans serovar Canicola Hond Utrecht IV en las cepas denominadas Dogy y Mayo. Las cepas denominadas Bel, Sarmiento, La Plata 4581 y La Plata 5478 mostraron un perfil idéntico al genotipo de L. interrogans serovar Portlandvere MY 1039. La cepa denominada Avellaneda presentó un perfil idéntico al genotipo L. interrogans serovar Icterohaemorrhagiae RGA, y la cepa denominada SB mostró un perfil idéntico al genotipo de L. interrogans serovar Pomona Baires y similar al serovar Pomona Pomona. Sería de gran utilidad incluir un mayor número de cepas provenientes de distintas poblaciones caninas de diversas provincias de Argentina a fin de conocer los perfiles de las cepas circulantes en el país. La información así obtenida contribuirá al control de la leptospirosis en la población canina


Leptospirosis is an infectious disease of wide global distribution, which is endemic in Argentina. The objective of this study was to obtain the genetic profiles of Leptospira spp. strains isolated from clinical cases of dogs in the province of Buenos Aires by the multiple-locus variable-number tandem repeat analysis (MLVA). Eight isolated canine strains were genotyped by MLVA, obtaining the identical profile of Leptospira interrogans serovar Canicola Hond Utrecht IV in the strains named Dogy and Mayo. The strains named Bel, Sarmiento, La Plata 4581 and La Plata 5478 were identical to the profile of the genotype of L. interrogans serovar Portlandvere MY 1039.The strain named Avellaneda was identical to the genotype profile of L. interrogans serovar Icterohaemorrhagiae RGA and the strain named SB had the same profile as the L. interrogans serovar Pomona Baires genotype and was similar to the profile of serovar Pomona Pomona genotype. It would be useful to include a larger number of isolates from different dog populations in various provinces of Argentina and to characterize the genetic profiles of the strains circulating in the country. The information obtained will be useful for the control of leptospirosis in the dog population


Asunto(s)
Animales , Perros , Argentina/epidemiología , Leptospira interrogans serovar canicola/aislamiento & purificación , Leptospira interrogans serovar canicola/genética , Repeticiones de Minisatélite/genética , Leptospira interrogans serovar icterohaemorrhagiae/aislamiento & purificación , Leptospira interrogans serovar icterohaemorrhagiae/genética , Leptospira interrogans serovar pomona/aislamiento & purificación , Leptospira interrogans serovar pomona/genética , Tipificación de Secuencias Multilocus , Leptospirosis/prevención & control
2.
Mem. Inst. Oswaldo Cruz ; 109(2): 163-167, abr. 2014. tab
Artículo en Inglés | LILACS | ID: lil-705823

RESUMEN

Leptospirosis is the most widespread zoonosis in the world and significant efforts have been made to determine and classify pathogenic Leptospira strains. This zoonosis is maintained in nature through chronic renal infections of carrier animals, with rodents and other small mammals serving as the most important reservoirs. Additionally, domestic animals, such as livestock and dogs, are significant sources of human infection. In this study, a multiple-locus variable-number tandem repeat analysis (MLVA) was applied to genotype 22 pathogenic Leptospira strains isolated from urban and periurban rodent populations from different regions of Argentina. Three MLVA profiles were identified in strains belonging to the species Leptospira interrogans (serovars Icterohaemorrhagiae and Canicola); one profile was observed in serovar Icterohaemorrhagiae and two MLVA profiles were observed in isolates of serovars Canicola and Portlandvere. All strains belonging to Leptospira borgpetersenii serovar Castellonis exhibited the same MLVA profile. Four different genotypes were isolated from urban populations of rodents, including both mice and rats and two different genotypes were isolated from periurban populations.


Asunto(s)
Animales , Ratones , Ratas , Leptospira/genética , Roedores/microbiología , Argentina , Didelphis/microbiología , Genotipo , Técnicas de Genotipaje/métodos , Leptospira interrogans serovar canicola/genética , Leptospira interrogans serovar icterohaemorrhagiae/genética , Leptospira interrogans serovar pomona/genética , Leptospira/clasificación , Leptospira/aislamiento & purificación , Leptospirosis/transmisión , Serogrupo , Serotipificación , Secuencias Repetidas en Tándem/genética , Población Urbana , Virulencia/genética
3.
Mem Inst Oswaldo Cruz ; 109(2): 163-7, 2014 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-24676656

RESUMEN

Leptospirosis is the most widespread zoonosis in the world and significant efforts have been made to determine and classify pathogenic Leptospira strains. This zoonosis is maintained in nature through chronic renal infections of carrier animals, with rodents and other small mammals serving as the most important reservoirs. Additionally, domestic animals, such as livestock and dogs, are significant sources of human infection. In this study, a multiple-locus variable-number tandem repeat analysis (MLVA) was applied to genotype 22 pathogenic Leptospira strains isolated from urban and periurban rodent populations from different regions of Argentina. Three MLVA profiles were identified in strains belonging to the species Leptospira interrogans (serovars Icterohaemorrhagiae and Canicola); one profile was observed in serovar Icterohaemorrhagiae and two MLVA profiles were observed in isolates of serovars Canicola and Portlandvere. All strains belonging to Leptospira borgpetersenii serovar Castellonis exhibited the same MLVA profile. Four different genotypes were isolated from urban populations of rodents, including both mice and rats and two different genotypes were isolated from periurban populations.


Asunto(s)
Leptospira/genética , Roedores/microbiología , Animales , Argentina , Didelphis/microbiología , Genotipo , Técnicas de Genotipaje/métodos , Leptospira/clasificación , Leptospira/aislamiento & purificación , Leptospira interrogans serovar canicola/genética , Leptospira interrogans serovar icterohaemorrhagiae/genética , Leptospira interrogans serovar pomona/genética , Leptospirosis/transmisión , Ratones , Ratas , Serogrupo , Serotipificación , Secuencias Repetidas en Tándem/genética , Población Urbana , Virulencia/genética
4.
Acta Trop ; 126(2): 164-6, 2013 May.
Artículo en Inglés | MEDLINE | ID: mdl-23435256

RESUMEN

Leptospirosis is a worldwide zoonotic infection caused by pathogenic Leptospira. Synanthropic rodents are recognized carriers of leptospires; however, the role of wild rodents in the epidemiology of the disease is still incipient. In this work, we describe Leptospira strain isolated from Cavia aperea (Brazilian guinea pig). The isolated strain was characterized by partial rpoB gene sequencing, variable-number tandem-repeats and histopathological analysis. The strain was identified as Leptospira interrogans, serogroup Icterohaemorrhagiae and caused clinical signs of leptospirosis in the hamster model, attesting to its virulence. In conclusion, these findings could be useful for elucidating the epidemiological role of C. aperea in leptospirosis.


Asunto(s)
Cobayas/microbiología , Leptospira interrogans serovar icterohaemorrhagiae/aislamiento & purificación , Leptospirosis/veterinaria , Enfermedades de los Roedores/microbiología , Animales , Proteínas Bacterianas/genética , Brasil/epidemiología , Cricetinae , ADN Bacteriano/química , ADN Bacteriano/genética , Marcadores Genéticos , Riñón/microbiología , Riñón/patología , Leptospira interrogans serovar icterohaemorrhagiae/clasificación , Leptospira interrogans serovar icterohaemorrhagiae/genética , Leptospira interrogans serovar icterohaemorrhagiae/patogenicidad , Leptospirosis/epidemiología , Leptospirosis/microbiología , Mesocricetus , Repeticiones de Minisatélite/genética , Enfermedades de los Roedores/epidemiología , Análisis de Secuencia de ADN , Virulencia , Zoonosis
5.
Braz. j. microbiol ; Braz. j. microbiol;44(1): 165-170, 2013. ilus
Artículo en Inglés | LILACS | ID: lil-676904

RESUMEN

Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two leptospires in female hamsters and a histopathological examination of infected animals found typical lesions associated with severe leptospirosis, including renal epithelium degeneration, hepatic karyomegaly, liver-plate disarray and lymphocyte infiltration. This highly virulent strain is now available for use in further studies, especially evaluation of vaccine candidates.


Asunto(s)
Ratas , Secuencia de Bases , Genoma Bacteriano , Técnicas In Vitro , Mucosa Intestinal , Leptospira interrogans serovar icterohaemorrhagiae/genética , Leptospira interrogans serovar icterohaemorrhagiae/aislamiento & purificación , Reacción en Cadena de la Polimerasa , Área Urbana , Enfermedad de Weil , Cricetinae , Técnicas Histológicas , Métodos , Piscinas , Virulencia
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