RESUMEN
Mitotic spindle orientation must be tightly regulated during development and adult tissue homeostasis. It determines cell-fate specification and tissue architecture during asymmetric and symmetric cell division, respectively. Here, we uncover a novel role for Ephrin-Eph intercellular signaling in controlling mitotic spindle alignment in Drosophila optic lobe neuroepithelial cells through aPKC activity-dependent myosin II regulation. We show that conserved core components of the mitotic spindle orientation machinery, including Discs Large1, Mud/NuMA, and Canoe/Afadin, mislocalize in dividing Eph mutant neuroepithelial cells and produce spindle alignment defects in these cells when they are down-regulated. In addition, the loss of Eph leads to a Rho signaling-dependent activation of the PI3K-Akt1 pathway, enhancing cell proliferation within this neuroepithelium. Hence, Eph signaling is a novel extrinsic mechanism that regulates both spindle orientation and cell proliferation in the Drosophila optic lobe neuroepithelium. Similar mechanisms could operate in other Drosophila and vertebrate epithelia.
Asunto(s)
Polaridad Celular , Proliferación Celular , Proteínas de Drosophila/metabolismo , Proteínas de la Membrana/metabolismo , Células Neuroepiteliales/enzimología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Huso Acromático/enzimología , Animales , Proteínas de Drosophila/genética , Drosophila melanogaster , Regulación del Desarrollo de la Expresión Génica , Regulación Enzimológica de la Expresión Génica , Proteínas de la Membrana/genética , Mutación , Miosina Tipo II/genética , Miosina Tipo II/metabolismo , Lóbulo Óptico de Animales no Mamíferos/citología , Fosfatidilinositol 3-Quinasa/genética , Fosfatidilinositol 3-Quinasa/metabolismo , Proteína Quinasa C/genética , Proteína Quinasa C/metabolismo , Proteínas Proto-Oncogénicas c-akt/genética , Proteínas Proto-Oncogénicas c-akt/metabolismo , Transducción de Señal , Huso Acromático/genética , Proteínas Supresoras de Tumor/genética , Proteínas Supresoras de Tumor/metabolismoRESUMEN
CTP synthase (CTPsyn) is a metabolic enzyme responsible for the de novo synthesis of the nucleotide CTP. Several recent studies have shown that CTPsyn forms filamentous subcellular structures known as cytoophidia in bacteria, yeast, fruit flies and humans. However, it remains elusive whether and how CTPsyn and cytoophidia play a role during development. Here, we show that cytoophidia are abundant in the neuroepithelial stem cells in Drosophila optic lobes. Optic lobes are underdeveloped in CTPsyn mutants as well as in CTPsyn RNAi. Moreover, overexpressing CTPsyn impairs the development of optic lobes, specifically by blocking the transition from neuroepithelium to neuroblast. Taken together, our results indicate that CTPsyn is critical for optic lobe homeostasis in Drosophila.
Asunto(s)
Ligasas de Carbono-Nitrógeno/metabolismo , Drosophila melanogaster/enzimología , Homeostasis , Lóbulo Óptico de Animales no Mamíferos/metabolismo , Animales , Encéfalo/citología , Encéfalo/embriología , Encéfalo/enzimología , Ligasas de Carbono-Nitrógeno/genética , Drosophila melanogaster/citología , Drosophila melanogaster/embriología , Drosophila melanogaster/genética , Larva/citología , Larva/enzimología , Larva/crecimiento & desarrollo , Mutación , Células-Madre Neurales/enzimología , Células-Madre Neurales/metabolismo , Células Neuroepiteliales/citología , Neuroglía/enzimología , Neuroglía/metabolismo , Lóbulo Óptico de Animales no Mamíferos/citología , Lóbulo Óptico de Animales no Mamíferos/enzimologíaRESUMEN
Long-chain acyl-CoA synthetases (ACSLs) convert long-chain fatty acids to acyl-CoAs, the activated substrates essential in various metabolic and signaling pathways. Mutations in ACSL4 are associated with non-syndromic X-linked mental retardation (MRX). However, the developmental functions of ACSL4 and how it is involved in the pathogenesis of MRX remain largely unknown. The Drosophila ACSL-like protein is highly homologous to human ACSL3 and ACSL4, and we designate it as dAcsl. In this study, we demonstrate that dAcsl and ACSL4 are highly conserved in terms of ACSL4's ability to substitute the functions of dAcsl in organismal viability, lipid storage and the neural wiring in visual center. In neurodevelopment, decapentaplegic (Dpp, a BMP-like molecule) production diminished specifically in the larval brain of dAcsl mutants. Consistent with the Dpp reduction, the number of glial cells and neurons dramatically decreased and the retinal axons mis-targeted in the visual cortex. All these defects in Drosophila brain were rescued by the wild-type ACSL4 but not by the mutant products found in MRX patients. Interestingly, expression of an MRX-associated ACSL4 mutant form in a wild-type background led to the lesions in visual center, suggesting a dominant negative effect. These findings validate Drosophila as a model system to reveal the connection between ACSL4 and BMP pathway in neurodevelopment, and to infer the pathogenesis of ACSL4-related MRX.
Asunto(s)
Coenzima A Ligasas/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/enzimología , Discapacidad Intelectual Ligada al Cromosoma X/enzimología , Percepción Visual , Secuencia de Aminoácidos , Animales , Encéfalo/enzimología , Encéfalo/metabolismo , Coenzima A Ligasas/química , Coenzima A Ligasas/genética , Modelos Animales de Enfermedad , Proteínas de Drosophila/genética , Drosophila melanogaster/química , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Humanos , Discapacidad Intelectual Ligada al Cromosoma X/genética , Discapacidad Intelectual Ligada al Cromosoma X/metabolismo , Discapacidad Intelectual Ligada al Cromosoma X/psicología , Datos de Secuencia Molecular , Lóbulo Óptico de Animales no Mamíferos/enzimología , Lóbulo Óptico de Animales no Mamíferos/metabolismo , Alineación de SecuenciaRESUMEN
This study provides the first immunohistochemical evidence visualizing cholinergic octopus neurons containing choline acetyltransferase (ChAT), the synthetic enzyme of acetylcholine. Because the antiserum applied here was raised against a recombinant protein encoded by exons 7 and 8 of the rat gene for ChAT, and initially used for studies in mammals, to validate antibody specificity for the octopus counterpart enzyme we therefore used three methods. Immunoprecipitation using Pansorbin indicated that immunoreactive octopus brain molecules were capable of synthesizing acetylcholine. Western blot analysis after denatured gel electrophoresis of octopus brain extracts revealed a single band at approximately 81 kDa. A gel slice containing the 81-kDa protein after native (nondenatured) gel electrophoresis exhibited high ChAT activity. All findings obtained with these three methods clearly indicated that the antiserum effectively recognizes octopus ChAT. The immunohistochemical use of the antiserum in the retina, optic lobe, and its neighboring peduncle complex detected enzyme-containing neuronal cell bodies in only two regions, the cell islands of the optic lobe medulla and the cortical layer of the posterior olfactory lobule. Immunoreactive fibers and probable nerve terminals were also found in the plexiform layer of the deep retina, within the stroma of the optic gland, and the neuropils of the optic lobe, peduncle lobe, and olfactory lobe. These results provide information on the morphology and distribution patterns of cholinergic neurons in the octopus visual system, a useful invertebrate model for learning and memory where the cholinergic system, as in higher vertebrates including mammals, plays an important role.
Asunto(s)
Acetilcolina/metabolismo , Colina O-Acetiltransferasa/metabolismo , Fibras Colinérgicas/enzimología , Fibras Nerviosas/enzimología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Tegmento Mesencefálico/enzimología , Animales , Western Blotting , Inmunohistoquímica , OctopodiformesRESUMEN
The Drosophila genes ninaB and ninaD, encoding a beta-carotene oxygenase and a type B scavenger receptor respectively, are essential for the biosynthesis of the 3-hydroxyretinal chromophore of rhodopsin. We analyzed transgenic reporter strains and performed in situ hybridization to show that both ninaB and ninaD are expressed in the adult brain but not retinal tissues. Developmental RT-PCR and tissue expression studies showed that ninaB is only expressed in the adult brain, while ninaD is expressed in the adult brain, the adult body, and many larval tissues. The data support a model in which NinaD is required for uptake and storage of dietary carotenoids throughout the larval and adult stages of development. Beta-carotene is transported to the adult brain, where cellular uptake by NinaD allows cleavage by the NinaB enzyme to produce retinal. Retinal is then transported to the retina for rhodopsin biogenesis.
Asunto(s)
Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Receptores Inmunológicos/genética , Receptores Inmunológicos/metabolismo , Vitamina A/metabolismo , beta-Caroteno 15,15'-Monooxigenasa/genética , beta-Caroteno 15,15'-Monooxigenasa/metabolismo , Factores de Edad , Animales , Animales Modificados Genéticamente , Encéfalo/enzimología , Encéfalo/fisiología , Drosophila melanogaster , Ganglios de Invertebrados/enzimología , Ganglios de Invertebrados/fisiología , Regulación Enzimológica de la Expresión Génica , Larva/enzimología , Larva/fisiología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Lóbulo Óptico de Animales no Mamíferos/fisiología , Retinaldehído/biosíntesis , Retinaldehído/química , Vitamina A/biosíntesis , beta Caroteno/química , beta Caroteno/farmacocinéticaRESUMEN
We previously characterized some crustacean glial cells by markers such as 2',3'-cyclic nucleotide 3'-phosphodiesterase and glial fibrillary acidic protein. Here we use antibodies against glutamine synthetase full-length molecule (anti-GS/FL), a GS C-terminal peptide (anti-GS/20aa-C), and brain S100 (anti-S100), as well as the binding of the insect glia and rat astrocytic marker Datura stramonium lectin (DSL), in the optic lobe of the prawn Macrobrachium rosenbergii. All markers label the lamina ganglionaris cartridge region (lighter: anti-GS/FL; heavier: DSL). In addition, anti-GS/FL labels superficial somata of external and internal medullas and internal chiasm cells. Both anti-GS/20aa-C and anti-S100 label heavily the glial sheaths of the lamina ganglionaris. In addition, anti-S100 binds to the perineurial glia of medullary parenchymal vessels. Western blot analyses show that both anti-GS/FL and anti-GS/20aa-C bind mostly to a band of 50-55 kDa, compatible with a long isoform of vertebrate GS, and accessorily to a possible dimer and, in the case of anti-GS/20aa-C, to an ill-defined band of intermediate mass. Binding of anti-S100 is selective for a single band of about 68 kDa but shows no protein in the weight range of the canonical S100 protein superfamily. DSL reveals two bands of about 75 and about 120 kDa, thus within the range of maximal recognition for rat astrocytes. Our results suggest that phenotype protein markers of the optic lobe glia share antigenic determinants with S100 and (a long form of) GS and that, similarly to vertebrate and insect glia, crustacean glia protein and N-glycan residue markers display regional heterogeneity.
Asunto(s)
Glutamato-Amoníaco Ligasa/inmunología , Neuroglía/enzimología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Palaemonidae/enzimología , Lectinas de Plantas/metabolismo , Proteínas S100/inmunología , Animales , Anticuerpos/metabolismo , Antígenos/inmunología , Biomarcadores/metabolismo , Membrana Celular/inmunología , Membrana Celular/metabolismo , Evolución Molecular , Glutamato-Amoníaco Ligasa/biosíntesis , Ácido Glutámico/metabolismo , Inmunohistoquímica/métodos , Peso Molecular , Neuroglía/citología , Neurópilo/inmunología , Neurópilo/metabolismo , Lóbulo Óptico de Animales no Mamíferos/citología , Palaemonidae/citología , Lectinas de Plantas/farmacocinética , Polisacáridos/inmunología , Unión Proteica/inmunología , Proteínas S100/biosíntesis , Especificidad de la EspecieRESUMEN
Guanine nucleotides are key players in mediating growth-cone signaling during neural development. The supply of cellular guanine nucleotides in animals can be achieved via the de novo synthesis and salvage pathways. The de novo synthesis of guanine nucleotides is required for lymphocyte proliferation in animals. Whether the de novo synthesis pathway is essential for any other cellular processes, however, remains unknown. In a search for genes required for the establishment of neuronal connectivity in the fly visual system, we identify the burgundy (bur) gene as an essential player in photoreceptor axon guidance. The bur gene encodes the only GMP synthetase in Drosophila that catalyzes the final reaction of de novo GMP synthesis. Loss of bur causes severe defects in axonal fasciculation, retinotopy, and growth-cone morphology, but does not affect photoreceptor differentiation or retinal patterning. Similar defects were observed when the raspberry (ras) gene, encoding for inosine monophosphate dehydrogenase catalyzing the IMP-to-XMP conversion in GMP de novo synthesis, was mutated. Our study thus provides the first in vivo evidence to support an essential and specific role for de novo synthesis of guanine nucleotides in axon guidance.
Asunto(s)
Axones/fisiología , Drosophila/fisiología , Guanosina Monofosfato/biosíntesis , Secuencia de Aminoácidos , Animales , Axones/enzimología , Axones/metabolismo , Tipificación del Cuerpo/genética , Tipificación del Cuerpo/fisiología , Ligasas de Carbono-Nitrógeno/genética , Ligasas de Carbono-Nitrógeno/fisiología , Diferenciación Celular/genética , Drosophila/genética , Drosophila/crecimiento & desarrollo , Evolución Molecular , Larva/enzimología , Larva/crecimiento & desarrollo , Larva/metabolismo , Datos de Secuencia Molecular , Mutación , Lóbulo Óptico de Animales no Mamíferos/enzimología , Lóbulo Óptico de Animales no Mamíferos/crecimiento & desarrollo , Células Fotorreceptoras de Invertebrados/enzimología , Células Fotorreceptoras de Invertebrados/metabolismo , Células Fotorreceptoras de Invertebrados/fisiología , Proteínas de Unión al GTP rac/genética , Proteínas de Unión al GTP rac/fisiologíaRESUMEN
In the fly's visual system, two classes of lamina interneuron, L1 and L2, cyclically change both their size and shape in a rhythm that is circadian. Several neurotransmitters and the lamina's glial cells are known to be involved in regulating these rhythms. Moreover, vacuolar-type H+-ATPase (V-ATPase) in the optic lobe is thought also to participate in such regulation. We have detected V-ATPase-like immunoreactivity in the heads of both Drosophilla melanogaster and Musca domestica using antibodies raised against either the B- or H-subunits of V-ATPase from D. melanogaster or against the B-subunit from two other insect species Culex quinquefasciatus and Manduca sexta. In the visual systems of both fly species V-ATPase was localized immunocytochemically to the compound eye photoreceptors. In D. melanogaster immunoreactivity oscillated during the day and night and under constant darkness the signal was stronger during the subjective night than the subjective day. In turn, blocking V-ATPase by injecting a V-ATPase blocker, bafilomycin, in M. domestica increased the axon sizes of L1 and L2, but only when bafilomycin was applied during the night. As a result bafilomycin abolished the day/night difference in axon size in L1 and L2, their sizes being similar during the day and night.
Asunto(s)
Dípteros/enzimología , Ojo/citología , Lóbulo Óptico de Animales no Mamíferos/enzimología , ATPasas de Translocación de Protón Vacuolares/metabolismo , Animales , Tamaño de la Célula , Culex/enzimología , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/enzimología , Proteínas de Insectos/metabolismo , Manduca/enzimología , Lóbulo Óptico de Animales no Mamíferos/citologíaRESUMEN
A requirement for nitric oxide (NO) in visual system development has been demonstrated in many model systems, but the role of potential downstream effector molecules has not been established. Developing Drosophila photoreceptors express an NO-sensitive soluble guanylate cyclase (sGC), whereas the optic lobe targets express NO synthase. Both of these molecules are expressed after photoreceptor outgrowth to the optic lobe, when retinal growth cones are actively selecting their postsynaptic partners. We have previously shown that inhibition of the NO-cGMP pathway in vitro leads to overgrowth of retinal axons. Here we examined flies mutant for the alpha subunit gene of the Drosophila sGC (Gcalpha1). This mutation severely reduced but did not abolish GCalpha1 protein levels and NO-stimulated sGC activity in the developing photoreceptors. Although few mutant individuals possessed a disorganized retinal projection pattern, pharmacological NOS inhibition during metamorphosis increased this disorganization in mutants to a greater degree than in the wild type. Adult mutants lacked phototactic behavior, and the off-transient component of electroretinograms was frequently absent or greatly reduced in amplitude. Normal phototaxis and off-transient amplitude were restored by heat shock-mediated Gcalpha1 expression applied during metamorphosis but not in the adult. We propose that diminished sGC activity in the visual system during development causes inappropriate or inadequate formation of first-order retinal synapses, leading to defects in visual system function and visually mediated behavior.
Asunto(s)
Guanilato Ciclasa/metabolismo , Lóbulo Óptico de Animales no Mamíferos/enzimología , Células Fotorreceptoras de Invertebrados/enzimología , Células Fotorreceptoras de Invertebrados/crecimiento & desarrollo , Vías Visuales/enzimología , Animales , Conducta Animal/efectos de los fármacos , Conducta Animal/fisiología , Drosophila , Electrorretinografía , Inhibidores Enzimáticos/farmacología , Guanilato Ciclasa/genética , Inmunohistoquímica , Técnicas In Vitro , Masculino , Metamorfosis Biológica/fisiología , Mutación , Óxido Nítrico Sintasa/antagonistas & inhibidores , Óxido Nítrico Sintasa/metabolismo , Lóbulo Óptico de Animales no Mamíferos/citología , Lóbulo Óptico de Animales no Mamíferos/crecimiento & desarrollo , Estimulación Luminosa , Células Fotorreceptoras de Invertebrados/citología , Subunidades de Proteína , Retina/citología , Retina/crecimiento & desarrollo , Retina/fisiología , Vías Visuales/citología , Vías Visuales/crecimiento & desarrolloRESUMEN
Nitric oxide (NO) is acknowledged as a messenger molecule in the nervous system. It has a role in the modulation of the chemosensory information and seems implicated also in visual processes and visually guided behaviour of some insects. In the present study, we used two different strains of the medfly Ceratitis capitata (Diptera, Tephritidae), a wild type eye colour and a white eye mutant line, as models to clarify the involvement of NO in the mature and developing visual system. The comparison between the pattern of enzyme histochemical localization of NO synthase (NOS), through NADPH diaphorase (NADPHd) staining, in the optic lobes of the two strains revealed for adults a stronger intensity of reaction in all the neuropiles and the sub-retinic monopolar cell layer of the wild type flies, with respect to the white eye mutant correspondent areas. Anti-NOS immunocytochemistry correlated with these results, underlying reactivity both in fine fibres and varicosities and in cell bodies and supporting the idea of presence of NOS also in the retina of the medfly optic lobes. NADPHd reactivity was present in the first developmental stages of the white eye mutant also, but at lower intensity than wild type, and it decreased in some areas during the transition to adult fly stage both in the wild type and in the white eye mutant. All these observations together indicate that changes in the NO system of C. capitata could be related to the visual information processing, when the visual response or discrimination are altered. Furthermore, NO may be involved in the establishment of the retinal projection pattern and in the control of optic lobes morphogenesis.
Asunto(s)
Dípteros/fisiología , Mutación , Óxido Nítrico Sintasa/análisis , Vías Visuales/fisiología , Animales , Dihidrolipoamida Deshidrogenasa/análisis , Dípteros/genética , Dípteros/crecimiento & desarrollo , Lóbulo Óptico de Animales no Mamíferos/citología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Lóbulo Óptico de Animales no Mamíferos/fisiología , Visión Ocular , Vías Visuales/citología , Vías Visuales/enzimología , Percepción VisualRESUMEN
N-acetyltransferase (NAT) and melatonin were determined in the optic lobe of the giant freshwater prawn Macrobrachium rosenbergii de Man. The prawns were divided into three groups: fast-growing "jumper" males; slow-growing "laggard" males; and females. Both NAT and melatonin levels in the jumper and laggard males were comparable, whereas those of the female were significantly lower. The results suggested a sexual dimorphism in the NAT and melatonin in the optic lobe of this species. It was also found that when one optic lobe was isolated, the level of NAT and melatonin in the contralateral optic lobe did not show a compensatory increase in either males or females. On the contrary, melatonin was suppressed in the remaining optic lobes in both sexes.
Asunto(s)
Arilamina N-Acetiltransferasa/análisis , Melatonina/análisis , Lóbulo Óptico de Animales no Mamíferos/química , Palaemonidae/química , Caracteres Sexuales , Análisis de Varianza , Animales , Femenino , Masculino , Lóbulo Óptico de Animales no Mamíferos/enzimología , Palaemonidae/enzimologíaRESUMEN
Development of the visual system in Drosophila requires the establishment of precise retinotopic connections between photoreceptors and their synaptic targets in the central nervous system. Nitric oxide (NO) has been implicated as a candidate signal involved in the establishment of retinal projection patterns. In this study the expression of NADPH-diaphorase in the lamina of Drosophila, and by implication nitric oxide synthase (NOS), was investigated in larvae with varying degrees of retinal innervation. NADPH-diaphorase expression was seen to increase in the lamina and eye disk following retinal neuronal death in eye specific pro-apoptotic larvae (pGMR-hid) compared to wild type larvae, and was lower in the lamina in absent or reduced retinal innervation mutants (eyes absent and sine oculis). Retinal innervation is seen to regulate the expression of NADPH-diaphorase expression in target structures.
Asunto(s)
Axones/fisiología , Drosophila melanogaster/embriología , Drosophila melanogaster/enzimología , NADPH Deshidrogenasa/metabolismo , Animales , Axones/enzimología , Drosophila melanogaster/fisiología , Larva/enzimología , Larva/crecimiento & desarrollo , Óxido Nítrico/fisiología , Lóbulo Óptico de Animales no Mamíferos/embriología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Retina/embriología , Retina/enzimologíaRESUMEN
In the optic lobe of the cephalopod mollusc Eledone moschata, two acetylcholinesterase forms I and II were detected, both showing a marked active site specificity with differently sized substrates. Catalytic efficiency (kcat/Km) of the prevailing form II is similar to that of acetylcholinesterases from vertebrate nervous system. Enzyme forms I and II were co-purified from a high-salt-Triton X-100 soluble extract of optic lobe by consecutive affinity chromatographies on procainamide- and concanavalin A-Sepharose columns and then separately obtained by preparative density gradient centrifugation. According to gel-filtration chromatography, sedimentation analysis and SDS-PAGE, the major form II is an amphiphilic globular dimer (135-136 kDa, 6.3-7.4 S) of monomers (66 kDa) S-S linked between terminal segments. Phosphatidylinositol anchors give cell membrane insertion, self-aggregation and detergent (Triton X-100, Brij 97) interaction. Form I, characterized only in part owing to its small amount, showed molecular size (129 kDa) and sedimentation coefficient (7.5 S) similar to those of form II; it is likely to be attached to the cell membrane by electrostatic interactions. Both forms behaved similarly with various inhibitors and underwent excess-substrate inhibition. The results obtained suggest a common origin of both form I and II from a single gene. The former could be a degradation product of the prevailing one (II), which is likely to be functional in cholinergic synapses.
Asunto(s)
Acetilcolinesterasa/metabolismo , Isoenzimas/metabolismo , Moluscos/enzimología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Acetilcolinesterasa/química , Acetilcolinesterasa/aislamiento & purificación , Animales , Catálisis , Centrifugación por Gradiente de Densidad , Disulfuros/química , Histocitoquímica , Isoenzimas/química , Isoenzimas/aislamiento & purificación , Cinética , Estructura Molecular , Peso Molecular , Especificidad por Sustrato , Fosfolipasas de Tipo C/farmacologíaRESUMEN
Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities, measured in 10 central regions of young, middle-aged and old cuttlefish, showed a regional heterogeneity but with different age-related distribution patterns. Maximal acetylcholine synthesis and catabolism were observed in the inferior frontal and the optic lobes. Important age-related decreases in ChAT activities were evidenced in most regions, while only moderate variations were found for AChE. Since the superior frontal lobe is involved in visual learning, the dramatic decrease in ChAT activity observed in this lobe (-77%) could be implicated in the learning deficits reported in senescent Sepia.
Asunto(s)
Acetilcolina/biosíntesis , Acetilcolina/metabolismo , Envejecimiento/fisiología , Moluscos/fisiología , Acetilcolinesterasa/metabolismo , Animales , Colina O-Acetiltransferasa/metabolismo , Femenino , Ganglios de Invertebrados/enzimología , Masculino , Lóbulo Óptico de Animales no Mamíferos/enzimologíaRESUMEN
The DC2 gene was isolated previously on the basis of sequence similarity to DC0, the major Drosophila protein kinase A (PKA) catalytic subunit gene. We show here that the 67-kD Drosophila DC2 protein behaves as a PKA catalytic subunit in vitro. DC2 is transcribed in mesodermal anlagen of early embryos. This expression depends on dorsal but on neither twist nor snail activity. DC2 transcriptional fusions mimic this embryonic expression and are also expressed in subsets of cells in the optic lamina, wing disc and leg discs of third instar larvae. A saturation screen of a small deficiency interval containing DC2 for recessive lethal mutations yielded no DC2 alleles. We therefore isolated new deficiencies to generate deficiency trans-heterozygotes that lacked DC2 activity. These animals were viable and fertile. The absence of DC2 did not affect the viability or phenotype of imaginal disc cells lacking DC0 activity or embryonic hatching of animals with reduced DC0 activity. Furthermore, transgenes expressing DC2 from a DC0 promoter did not efficiently rescue a variety of DC0 mutant phenotypes. These observations indicate that DC2 is not an essential gene and is unlikely to be functionally redundant with DC0, which has multiple unique functions during development.
Asunto(s)
Proteínas Quinasas Dependientes de AMP Cíclico/genética , Proteínas de Drosophila , Drosophila/genética , Factores de Transcripción , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Catálisis , Proteínas Quinasas Dependientes de AMP Cíclico/metabolismo , ADN Complementario , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Drosophila/embriología , Drosophila/enzimología , Regulación del Desarrollo de la Expresión Génica , Genes Reporteros , Datos de Secuencia Molecular , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Lóbulo Óptico de Animales no Mamíferos/embriología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Fenotipo , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Factores de Transcripción de la Familia Snail , Proteína 1 Relacionada con TwistRESUMEN
Choline acetyltransferase (ChAT) is the enzyme catalyzing the biosynthesis of acetylcholine and is considered to be a phenotypically specific marker for cholinergic neurons. We have examined the distribution of ChAT-expressing neurons in the larval nervous system of Drosophila melanogaster by three different but complementary techniques: in situ hybridization with a cRNA probe to ChAT messenger RNA, immunocytochemistry using a monoclonal anti-ChAT antibody, and X-gal staining of transformed animals carrying a reporter gene composed of 7.4 kb of 5' flanking DNA from the ChAT gene fused to a lacZ reporter gene. All three techniques demonstrated ChAT-expressing neurons in the larval visual system. In embryos, the photoreceptor organ (Bolwig's organ) exhibited strong cRNA hybridization signals. The optic lobe of late third-instar larvae displayed ChAT immunoreactivity in Bolwig's nerve and a neuron close to the insertion site of the optic stalk. This neuron's axon ran in parallel with Bolwig's nerve to the larval optic neuropil. This neuron is likely to be a first-order interneuron of the larval visual system. Expression of the lacZ reporter gene was also detected in Bolwig's organ and the neuron stained by anti-ChAT antibody. Our observations indicate that acetylcholine may be a neurotransmitter in the larval photoreceptor cells as well as in a first-order interneuron in the larval visual system of Drosophila melanogaster.
Asunto(s)
Colina O-Acetiltransferasa/metabolismo , Drosophila melanogaster/enzimología , Neuronas/enzimología , Animales , Anticuerpos Monoclonales , Encéfalo/enzimología , Tamaño de la Célula , Colina O-Acetiltransferasa/genética , Colina O-Acetiltransferasa/inmunología , ADN Complementario , Embrión no Mamífero/enzimología , Galactósidos , Genes Reporteros , Inmunohistoquímica , Hibridación in Situ , Indoles , Operón Lac , Larva/enzimología , Neuronas/citología , Lóbulo Óptico de Animales no Mamíferos/enzimología , Pupa/enzimología , ARN Mensajero/análisis , Transformación Genética , Vías Visuales/fisiología , beta-Galactosidasa/genéticaRESUMEN
While steady-state kinetic parameters (metabolite pools, Km and activation energies) are partially known for the enzymes involved in phosphatidylcholine synthesis and degradation in mammalian brain, they are not available for the nervous system of lower vertebrates or invertebrates. Since the extent of evolutionary development of an enzyme is not known a priori, we evaluated the kinetic and thermodynamic parameters of choline kinase, CTP:phosphocholine cytidylyltransferase, choline phosphotransferase and glycerophosphorylcholine phosphodiesterase in squid (Loligo pealei) optic lobe, dogfish (Mustelus canis) and rat brain. For all these enzyme activities, basic similarities in Km and inhibitor effect were found. The same was true for the activation energies Ea, with the exception of squid choline kinase and dogfish cytidylyltransferase. Treatment of microsomal membranes with phospholipase C sharply inhibited cytidylyltransferase activity in all three animal species. In dogfish brain, glycerophosphorylcholine phosphodiesterase activity was undetectable. Our results are consistent with the notion that the kinetic properties of the enzyme activities leading to the preservation of the phosphatidylcholine membranous pool may have appeared early in metazoan evolution and been fully conserved in mammals.
Asunto(s)
Evolución Biológica , Encéfalo/enzimología , Decapodiformes/metabolismo , Cazón/metabolismo , Lóbulo Óptico de Animales no Mamíferos/enzimología , Fosfatidilcolinas/metabolismo , Animales , Colina Quinasa/antagonistas & inhibidores , Colina Quinasa/química , Colina Quinasa/metabolismo , Citidililtransferasa de Colina-Fosfato , Diacilglicerol Colinafosfotransferasa/antagonistas & inhibidores , Diacilglicerol Colinafosfotransferasa/química , Activación Enzimática , Inhibidores Enzimáticos/farmacología , Cinética , Nucleotidiltransferasas/antagonistas & inhibidores , Nucleotidiltransferasas/química , Nucleotidiltransferasas/metabolismo , Hidrolasas Diéster Fosfóricas/química , Hidrolasas Diéster Fosfóricas/metabolismo , Ratas , Termodinámica , Fosfolipasas de Tipo C/farmacologíaRESUMEN
We have analyzed the cis-regulatory regions in the 5' flanking DNA of the Drosophila melanogaster choline acetyltransferase (ChAT; E.C. 2.3.1.6) gene by using germline transformants. These transformants are carrying wild-type ChAT cDNA fused to different lengths of 5' flanking sequence of the ChAT gene. Appropriate genetic crosses were used to introduce the transgene into animals with a presumptive null genetic background for endogenous ChAT. Expression of ChAT protein could thus be attributed exclusively to the transgene. Using a monoclonal antibody against Drosophila ChAT, we have investigated the spatial distribution of transgenic ChAT and compared it to the normal distribution of ChAT protein in wild-type animals. The brains of 7.4 kb cDNA transformants showed a ChAT expression pattern similar to that of wild-type animals in the first- and second-order sensory neuropil but reduced expression in other highly ordered neuropil. Several lines that were transformed with 1.2 kb or 0.8 kb of 5' flanking DNA demonstrated relatively normal expression in sensory neuropil. In addition, these lines also showed ectopic expression in higher order neuropil. In the optic lobe, the expression pattern directed by 7.4 kb of 5' flanking DNA was very similar to that of wild-type ChAT expression. In contrast, 1.2 kb or 0.8 kb transformants showed reduced levels of expression and a more limited pattern of distribution in the optic lobe. Our results suggest that the 5' flanking DNA of the ChAT gene can be divided into several separable positive and negative regulatory regions, which define various subsets of cholinergic neurons in the nervous system.
Asunto(s)
Colina O-Acetiltransferasa/genética , ADN Complementario/genética , Drosophila melanogaster/enzimología , Regulación Enzimológica de la Expresión Génica/fisiología , Secuencias Reguladoras de Ácidos Nucleicos , Transformación Genética , Animales , Animales Modificados Genéticamente , Conducta Animal/fisiología , Colina O-Acetiltransferasa/análisis , Genes Reporteros , Inmunohistoquímica , Lóbulo Óptico de Animales no Mamíferos/enzimología , Fenotipo , Valores de ReferenciaRESUMEN
We identified two Drosophila genes (dgc alpha 1 and dgc beta 1) that encode the soluble guanylyl cyclase alpha and beta subunits, respectively. The putative Dgc alpha 1 protein is 76 kDa, has 35% amino acid identity with previously isolated alpha subunits, and was immunolocalized to the adult retina, to the optic lobes, and throughout the brain neuropil. The Dgc beta 1 protein is 86 kDa and exhibits 59% amino acid identity with the rat beta 1 protein. However, the Dgc beta 1 protein has an additional 118 amino acids inserted near the amino terminus, which makes it significantly larger than the rat beta 1. The Dgc beta 1 protein was immunolocalized to the optic lobes and throughout the brain neuropil, with no detectable expression in the retina. The Dgc alpha 1 and Dgc beta 1 cDNAs were stably transfected into human kidney 293 cells. Expression of the individual subunits and mixing of the individually expressed subunits failed to generate significant guanylyl cyclase activity. Only coexpression of the subunits resulted in significant guanylyl cyclase activity. Our results indicate that Dgc alpha 1 and Dgc beta 1 are soluble guanylyl cyclase alpha and beta subunits that are capable of forming a functional guanylyl cyclase heterodimer.
Asunto(s)
Drosophila/enzimología , Drosophila/genética , Genes de Insecto , Guanilato Ciclasa/biosíntesis , Guanilato Ciclasa/genética , Secuencia de Aminoácidos , Animales , Encéfalo/enzimología , Línea Celular , ADN Complementario/metabolismo , Técnica del Anticuerpo Fluorescente , Guanilato Ciclasa/análisis , Humanos , Inmunohistoquímica , Hibridación in Situ , Riñón , Pulmón/enzimología , Sustancias Macromoleculares , Datos de Secuencia Molecular , Peso Molecular , Lóbulo Óptico de Animales no Mamíferos/enzimología , Especificidad de Órganos , Ratas , Proteínas Recombinantes/análisis , Proteínas Recombinantes/biosíntesis , Mapeo Restrictivo , Homología de Secuencia de Aminoácido , TransfecciónRESUMEN
Giant freshwater prawns, Macrobrachium rosenbergii de Man, were reared under three different lighting conditions: continuous darkness (DD), 12 hr of light and 12 hr of darkness (LD 12:12) and continuous light (LL). After one month, the prawns were sacrificed and optic lobes isolated from the eyestalks were determined for N-acetyltransferase (NAT) activities and melatonin concentrations. Gonads were weighed and examined under light microscopy. The optic lobes from LL prawns contained significantly higher activities of NAT than those from LD 12:12 prawns. The melatonin concentrations and size and histological features of the gonads from the three groups of prawns did not differ. The results indicate that continuous light increases NAT activities in the optic lobe of M. rosenbergii but has no drastic effect on gonadal growth.