RESUMEN
The explosion in one of the four reactors of the Chernobyl Nuclear Power Plant (CNPP, Chernobyl) caused the worst nuclear environmental disaster ever seen. Currently, 23 years after the accident, the soil in the close vicinity of CNPP is still significantly contaminated with long-living radioisotopes, such as (137)Cs. Despite this contamination, the plants growing in Chernobyl area were able to adapt to the radioactivity, and survive. The aim of this study was to investigate plant adaptation mechanisms toward permanently increased level of radiation using a quantitative high-throughput proteomics approach. Soybeans of a local variety (Soniachna) were sown in contaminated and control fields in the Chernobyl region. Mature seeds were harvested and the extracted proteins were subjected to two-dimensional gel electrophoresis (2-DE). In total, 9.2% of 698 quantified protein spots on 2-D gel were found to be differentially expressed with a p-value
Asunto(s)
Adaptación Fisiológica , Accidente Nuclear de Chernóbil , Glycine max/fisiología , Proteínas de Plantas/metabolismo , Semillas/fisiología , Contaminantes Radiactivos del Suelo/toxicidad , Isótopos de Cesio/toxicidad , Electroforesis en Gel Bidimensional , Expresión Génica/efectos de los fármacos , Geografía , Modelos Biológicos , Proteómica , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Semillas/efectos de la radiación , Glycine max/metabolismo , Glycine max/efectos de la radiación , Estrés Fisiológico , Espectrometría de Masas en Tándem , Factores de Tiempo , UcraniaRESUMEN
Under the conventional cytogenetical examination of 6 different children groups (with and without non-stohastic thyroid pathology, exposed and nonexposed to the iodine isotopes in 1986) from the goiter endemic zone of Ukraine which belongs to the territory contaminated by 137Cs radionuclides, the identical to the spontaneous level of somatic chromosome mutagenesis of the last decade in all observed groups with the tendency to increasing of stable aberrations in some persons had been established.