RESUMEN
PURPOSE: To evaluate the effects of photobiomodulation therapy (PBMT) at 685 nm on diabetic wound healing in rats suffering from bacterial infection induced by Staphylococcus aureus (S. aureus). METHODS: Thirty streptozotocin-induced diabetic rats were allocated into two groups: control and PBMT. A 4-cm full-thickness linear-incision was made on the dorsal midline and was contaminated with S. aureus. The wounds in the PBMT group were irradiated daily for 5 consecutive days, starting 3 days after the induction and always in the mornings. RESULTS: The result revealed that PBMT resulted in a significant decrease in S. aureus CFU in the PBMT group in comparison to the control group (P<0.05). The length of wounds, in the 2nd and 3rd weeks, in the PBMT group were significantly shorter compared to the control group (P<0.05). PBMT caused a significant increase in the histological parameters in comparison to the control group (P<0.05). Moreover, PBMT significantly increased the breaking strength of the surgical scars produced in the skin of the PBMT group when compared to the control group (P<0.05). CONCLUSION: Photobiomodulation therapy may be useful in the management of wound infection through a significant bacterial growth inhibition and an acceleration of wound healing process.
Asunto(s)
Diabetes Mellitus Experimental/complicaciones , Terapia por Luz de Baja Intensidad/métodos , Infecciones Estafilocócicas/radioterapia , Infección de la Herida Quirúrgica/radioterapia , Cicatrización de Heridas/efectos de la radiación , Animales , Diabetes Mellitus Experimental/inducido químicamente , Modelos Animales de Enfermedad , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/efectos de la radiación , Infección de la Herida Quirúrgica/microbiologíaRESUMEN
ABSTRACT PURPOSE: To evaluate the effects of photobiomodulation therapy (PBMT) at 685 nm on diabetic wound healing in rats suffering from bacterial infection induced by Staphylococcus aureus (S. aureus). METHODS: Thirty streptozotocin-induced diabetic rats were allocated into two groups: control and PBMT. A 4-cm full-thickness linear-incision was made on the dorsal midline and was contaminated with S. aureus. The wounds in the PBMT group were irradiated daily for 5 consecutive days, starting 3 days after the induction and always in the mornings. RESULTS: The result revealed that PBMT resulted in a significant decrease in S. aureus CFU in the PBMT group in comparison to the control group (P<0.05). The length of wounds, in the 2nd and 3rd weeks, in the PBMT group were significantly shorter compared to the control group (P<0.05). PBMT caused a significant increase in the histological parameters in comparison to the control group (P<0.05). Moreover, PBMT significantly increased the breaking strength of the surgical scars produced in the skin of the PBMT group when compared to the control group (P<0.05). CONCLUSION: Photobiomodulation therapy may be useful in the management of wound infection through a significant bacterial growth inhibition and an acceleration of wound healing process.
Asunto(s)
Animales , Masculino , Ratas , Infecciones Estafilocócicas/radioterapia , Infección de la Herida Quirúrgica/radioterapia , Cicatrización de Heridas/efectos de la radiación , Terapia por Luz de Baja Intensidad/métodos , Diabetes Mellitus Experimental/complicaciones , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/efectos de la radiación , Infección de la Herida Quirúrgica/microbiología , Distribución Aleatoria , Ratas Wistar , Diabetes Mellitus Experimental/inducido químicamente , Modelos Animales de EnfermedadRESUMEN
AIM: We aimed to assess the use of two wavelengths on the healing of infected wounds. BACKGROUND: Infection is the most significant cause of impaired wound repair or healing. Several therapeutic approaches are used for improving wound healing including the use of different light sources, such as the laser. Some wavelengths yield positive photobiological effects on the healing process. MATERIAL AND METHODS: The backs of 24 young adult male Wistar rats under general anesthesia were shaved and cleaned, and a 1 by 1 cm cutaneous wound was created with a scalpel and left untreated. The wounds were infected with Staphylococcus aureus, and the rats were randomly divided into two sets of four subgroups with three animals in each subgroup: control, red laser light, infrared laser light, and red + infrared laser light. Laser phototherapy was carried out with a diode [λ680 nm/790 nm, power (P) = 30 mW/40 mW, continuous wave laser, Ø = 3 mm, power density (P) = 424 and 566 mW/cm(2), time = 11.8/8.8 sec, E = 0.35 J] and started immediately after surgery and repeated every other day for 7 d. Laser light was applied on four points around the wounded area (5 J/cm(2)). The animals were killed either 8 or 15 d after contamination. Specimens were taken, embedded in paraffin, and sectioned and stained for histological analysis. RESULTS: Histological analysis showed that control subjects had a lower amount of blood vessels when compared with irradiated subjects. Irradiated subjects had more advanced resolution of inflammation compared with controls. Irradiated subjects also showed a more intense expression of the collagen matrix. The collagen fibers were mostly mature and well organized in these subjects at the end of the experimental time especially when both wavelengths were used. CONCLUSION: The results of the present study indicate that laser phototherapy has a positive effect on the healing of infected wounds, particularly with the association of λ680 + λ790 nm.