RESUMEN
A 48-year-old woman with fire-related inhalation injury had pink urine and a pink skin. This was caused by hydroxocobalamin, which was used to treat cyanide poisoning.
Asunto(s)
Hidroxocobalamina/orina , Femenino , Humanos , Hidroxocobalamina/efectos adversos , Hidroxocobalamina/uso terapéutico , Persona de Mediana Edad , Piel/patología , Lesión por Inhalación de Humo/tratamiento farmacológicoAsunto(s)
Antídotos/uso terapéutico , Artefactos , Hematuria , Hidroxocobalamina/uso terapéutico , Traumatismo Múltiple/orina , Urinálisis , Antídotos/farmacocinética , Intoxicación por Monóxido de Carbono/etiología , Intoxicación por Monóxido de Carbono/orina , Traumatismos Craneocerebrales/complicaciones , Traumatismos Craneocerebrales/orina , Incendios , Piromanía , Hematuria/orina , Humanos , Cianuro de Hidrógeno/envenenamiento , Hidroxocobalamina/farmacocinética , Hidroxocobalamina/orina , Masculino , Persona de Mediana Edad , Traumatismo Múltiple/complicaciones , Lesión por Inhalación de Humo/tratamiento farmacológico , Lesión por Inhalación de Humo/orina , Intento de Suicidio , Heridas por Arma de Fuego/orinaAsunto(s)
Cianuros/envenenamiento , Hematínicos/farmacología , Hidroxocobalamina/farmacología , Pigmentación de la Piel/efectos de los fármacos , Femenino , Hematínicos/orina , Humanos , Hidroxocobalamina/orina , Persona de Mediana Edad , Fotograbar , Intoxicación/diagnóstico , Intoxicación/tratamiento farmacológico , Intoxicación/etiología , Lesión por Inhalación de Humo/complicacionesRESUMEN
Hydroxocobalamin (OHCo) and cyanocobalamin (CNCo) are determined directly in biological media, without extraction, by using first derivative spectrophotometry. We diluted 200 mL of plasma, urine, or standards with 1.8 mL of pH 6 buffer (boric acid, potassium dihydrogen orthophosphate, and potassium hydroxide). The first derivative spectra of the dilutions were plotted between 320 and 400 nm. At the exact zero-crossing point for hydroxocobalamin, the derivative values of cyanocobalamin concentration were determined. The same procedure was followed for hydroxocobalamin at the zero-crossing point for cyanocobalamin. The derivative values of the concentration curves are linear in the range 5-100 microM. The minimum detection limit is approximately 5 microM for hydroxocobalamin of cyanocobalamin on the determination of hydroxocobalamin or vice versa, although the spectra strongly overlap. The method is fast and simple to use, thus making it easy to assess the in vivo transformation of hydroxocobalamin into cyanocobalamin after the administration of high doses of hydrocobalamin in cyanide poisoning.