RESUMEN
The movement of progesterone (P), cortisol (F) and 17-hydroxyprogesterone (17-OHP) across the blood-cerebrospinal fluid (CSF) barrier was determined using six adult male rhesus monkeys with indwelling canulae in the lateral ventricles of their brains. Tritiated steroids were given iv as a bolus followed by a constant 6 h infusion with continuous collection of CSF and periodic sampling of blood before and during the infusion. The amounts of authentic steroid in the plasma and CSF were determined by recrystallization to constant isotopic ratio and the amount of free plasma steroid was determined by equilibrium dialysis against Ringer's solution. Tritiated progesterone was undetectable in the pooled samples of CSF. The average concentration of tritiated 17-OHP in the CSF was 10.3% of the concurrent plasma level while the concentration of tritiated F was 22.5% of the concurrent plasma level. Plasma free steroid was found to be 2.2% for P, 6.3% for 17-OHP and 22.3% for F, showing a rough correlation between steroid entry into the CSF and free steroid concentration in plasma.
Asunto(s)
Barrera Hematoencefálica , Esteroides/metabolismo , Animales , Dihidrotestosterona/sangre , Dihidrotestosterona/líquido cefalorraquídeo , Estradiol/sangre , Estradiol/líquido cefalorraquídeo , Haplorrinos , Hidrocortisona/sangre , Hidrocortisona/líquido cefalorraquídeo , Hidroxiprogesteronas/sangre , Hidroxiprogesteronas/líquido cefalorraquídeo , Macaca mulatta , Masculino , Progesterona/sangre , Progesterona/líquido cefalorraquídeo , Testosterona/sangre , Testosterona/líquido cefalorraquídeoAsunto(s)
Barrera Hematoencefálica , Hormonas Esteroides Gonadales/líquido cefalorraquídeo , Noretinodrel/líquido cefalorraquídeo , Progestinas/líquido cefalorraquídeo , Animales , Haplorrinos , Hidroxiprogesteronas/líquido cefalorraquídeo , Inyecciones Intravenosas , Macaca , Mestranol/líquido cefalorraquídeo , Progesterona/líquido cefalorraquídeo , Testosterona/líquido cefalorraquídeo , TritioRESUMEN
PIP: Studies carried out in recent years, including those conducted by the author on the rhesus monkey, which have added more information on the possible cellular and humoral pathways by which the neural and endocrine functions concerned with reproductive phenomena are integrated are reviewed. The possible involvement of the circumventricular structures, especially the tanycyte ependyma and the pineal gland in the detection of the endogenous levels of gonadal hormones, and the cerebrospinal fluid (CSF) in the transmission of hormonal information to the various parts of the brain indicated further areas of research to be explored. Such exploration should be conducted in diverse, yet complimentary, disciplines so that the whole spectrum of events known to occur in the neuroendocrine regulation of reproduction can be studied.^ieng