RESUMEN

Utilizing molecular dynamics and free energy perturbation, we examine the relative binding affinity of several covalent polycyclic aromatic hydrocarbon - DNA (PAH-DNA) adducts at the central adenine of NRAS codon-61, a mutational hotspot implicated in cancer risk. Several PAHs classified by the International Agency for Research on Cancer as probable, possible, or unclassifiable as to carcinogenicity are found to have greater binding affinity than the known carcinogen, benzo[a]pyrene (B[a]P). van der Waals interactions between the intercalated PAH and neighboring nucleobases, and minimal disruption of the DNA duplex drive increases in binding affinity. PAH-DNA adducts may be repaired by global genomic nucleotide excision repair (GG-NER), hence we also compute relative free energies of complexation of PAH-DNA adducts with RAD4-RAD23 (the yeast ortholog of human XPC-RAD23) which constitutes the recognition step in GG-NER. PAH-DNA adducts exhibiting the greatest DNA binding affinity also exhibit the least RAD4-RAD23 complexation affinity and are thus predicted to resist the GG-NER machinery, contributing to their genotoxic potential. In particular, the fjord region PAHs dibenzo[a,l]pyrene, benzo[g]chrysene, and benzo[c]phenanthrene are found to have greater binding affinity while having weaker RAD4-RAD23 complexation affinity than their respective bay region analogs B[a]P, chrysene, and phenanthrene. We also find that the bay region PAHs dibenzo[a,j]anthracene, dibenzo[a,c]anthracene, and dibenzo[a,h]anthracene exhibit greater binding affinity and weaker RAD4-RAD23 complexation affinity than B[a]P. Thus, the study of PAH genotoxicity likely needs to be substantially broadened, with implications for public policy and the health sciences. This approach can be broadly applied to assess factors contributing to the genotoxicity of other unclassified compounds.

Asunto(s)

Aductos de ADN , Hidrocarburos Policíclicos Aromáticos , Hidrocarburos Policíclicos Aromáticos/toxicidad , Hidrocarburos Policíclicos Aromáticos/química , Hidrocarburos Policíclicos Aromáticos/metabolismo , Aductos de ADN/química , Aductos de ADN/metabolismo , Aductos de ADN/genética , Humanos , Reparación del ADN , Mutágenos/toxicidad , Mutágenos/química , Simulación de Dinámica Molecular , Proteínas de Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/química , Termodinámica , Benzo(a)pireno/toxicidad , Benzo(a)pireno/química , Benzo(a)pireno/metabolismo , ADN/química , ADN/metabolismo , Benzopirenos/toxicidad , Benzopirenos/química , Benzopirenos/metabolismo , Proteínas de Unión al ADN/metabolismo , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/química

RESUMEN

Riparian infiltration zones are crucial for maintaining water quality by reducing the aqueous concentrations of polycyclic aromatic hydrocarbons (PAHs) through adsorption and biodegradation within the aquatic ecosystem. Dissolved organic matter (DOM) are ubiquitous in riparian infiltration zones where they extensively engage in the adsorption and biodegradation of PAHs, thereby influencing PAHs natural attenuation potential within riparian infiltration zones. Few studies have explored the natural attenuation mechanisms of PAHs influenced by DOM in riparian infiltration zones. In this study, the natural attenuation mechanisms of naphthalene (a typical PAHs component), under the influence of DOM, were explored, based on a case riverside source area. Analysis of microbial community structures, and the electron acceptor (e.g., Fe(III), DO/NO3-, SO42-)/electron donor (naphthalene and DOM) concentration changes within the riparian infiltration zone revealed a competitive inhibition relationship between DOM and naphthalene during microbial metabolism. Biodegradation experiments showed that when the concentration of DOM is higher than 4.0 mg·L-1, it inhibits the biodegradation of naphthalene. DOM competitively inhibits the biodegradation of naphthalene through the following mechanisms: (i) triggering microbial antioxidative defense mechanisms, diminishing the available resources for microbial participation in naphthalene degradation; (ii) altering microbial community structure; (iii) modulating microbial EPS composition, reducing the efficiency of microorganisms in utilizing carbon sources; and (iv) inhibiting the expression levels of downstream genes involved in naphthalene degradation. The competitive inhibition constants of DOM with concentrations of 1.0, 2.0, 4.0, 8.0, and 16.0 mg·L-1 on naphthalene biodegradation are -2.0 × 10-3, -5.0 × 10-3,1.0 × 10-3, 4.0 × 10-4, and 1.0 × 10-4, respectively. These findings enhance understanding of PAHs attenuation in riparian infiltration zone, providing a basis for assessing and managing PAHs pollution risks during riparian extraction.

Asunto(s)

Biodegradación Ambiental , Naftalenos , Contaminantes Químicos del Agua , Hidrocarburos Policíclicos Aromáticos/metabolismo , Ríos

RESUMEN

BACKGROUND: Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable. Predation is a key top-down control mechanism affecting inoculum establishment, however, its effects on this process have received little attention. This study focused on the impact of trophic interactions on bioaugmentation success in two soils with different pollution exposure histories. We inoculated a 13C-labelled pollutant-degrading consortium in these soils and tracked the fate of the labelled biomass through stable isotope probing (SIP) of DNA. We identified active bacterial and eukaryotic inoculum-biomass consumers through amplicon sequencing of 16S rRNA and 18S rRNA genes coupled to a novel enrichment factor calculation. RESULTS: Inoculation effectively increased PAH removal in the short-term, but not in the long-term polluted soil. A decrease in the relative abundance of the inoculated genera was observed already on day 15 in the long-term polluted soil, while growth of these genera was observed in the short-term polluted soil, indicating establishment of the inoculum. In both soils, eukaryotic genera dominated as early incorporators of 13C-labelled biomass, while bacteria incorporated the labelled biomass at the end of the incubation period, probably through cross-feeding. We also found different successional patterns between the two soils. In the short-term polluted soil, Cercozoa and Fungi genera predominated as early incorporators, whereas Ciliophora, Ochrophyta and Amoebozoa were the predominant genera in the long-term polluted soil. CONCLUSION: Our results showed differences in the inoculum establishment and predator community responses, affecting bioaugmentation efficiency. This highlights the need to further study predation effects on inoculum survival to increase the applicability of inoculation-based technologies. Video Abstract.

Asunto(s)

Bacterias , Biodegradación Ambiental , ARN Ribosómico 16S , ARN Ribosómico 18S , Microbiología del Suelo , Contaminantes del Suelo , Suelo , Suelo/química , Contaminantes del Suelo/metabolismo , ARN Ribosómico 16S/genética , ARN Ribosómico 18S/genética , Bacterias/metabolismo , Bacterias/clasificación , Bacterias/genética , Biomasa , Isótopos de Carbono/metabolismo , Cadena Alimentaria , Hidrocarburos Policíclicos Aromáticos/metabolismo , Marcaje Isotópico

RESUMEN

Endophytic bacteria can promote plant growth and accelerate pollutant degradation. However, it is unclear whether endophytic consortia (Consortium_E) can stabilize colonisation and degradation. We inoculated Consortium_E into the rhizosphere to enhance endophytic bacteria survival and promote pollutant degradation. Rhizosphere-inoculated Consortium_E enhanced polycyclic aromatic hydrocarbon (PAH) degradation rates by 11.5-13.1 % compared with sole bioaugmentation and plant treatments. Stable-isotope-probing (SIP) showed that the rhizosphere-inoculated Consortium_E had the largest number of degraders (8 amplicon sequence variants). Furthermore, only microbes from Consortium_E were identified among the degraders in bioaugmentation treatments, indicating that directly participated in phenanthrene metabolism. Interestingly, Consortium_E reshaped the community structure of degraders without significantly altering the rhizosphere community structure, and strengthened the core position of degraders in the network, facilitating close interactions between degraders and non-degraders in the rhizosphere, which were crucial for ensuring stable functionality. The synergistic effect between plants and Consortium_E significantly enhanced the upregulation of aromatic hydrocarbon degradation and auxiliary degradation pathways in the rhizosphere. These pathways showed a non-significant increasing trend in the uninoculated rhizosphere compared with the control, indicating that Consortium_E primarily promotes rhizosphere effects. Our results explore the Consortium_E bioaugmentation mechanism, providing a theoretical basis for the ecological restoration of contaminated soils.

Asunto(s)

Biodegradación Ambiental , Medicago sativa , Hidrocarburos Policíclicos Aromáticos , Rizosfera , Contaminantes del Suelo , Hidrocarburos Policíclicos Aromáticos/metabolismo , Contaminantes del Suelo/metabolismo , Medicago sativa/microbiología , Medicago sativa/metabolismo , Microbiota , Endófitos/metabolismo , Microbiología del Suelo , Bacterias/metabolismo , Bacterias/genética

RESUMEN

Polycyclic aromatic hydrocarbons (PAHs) are highly concerning environmental pollutants due to their toxicity, persistence, and bioaccumulation. In this paper, concentrations and compositions of 16 United States Environmental Protection Agency (USEPA) priority control PAHs in the fish collected from four lakes in central and eastern China were analyzed. 18 species of fish were collected from four typical lakes, namely Taihu Lake, Danjiangkou Reservoir, Yuncheng Salt Lake, and Nansi Lake. Quantitative analysis of PAHs were carried out with gas chromatograph/mass spectrometer, and 13 out of 16 PAHs were identified, with the main components being pyrene, chrysene, naphthalene, and benzo(b)fluoranthene. The accumulation of PAHs in fish from Taihu Lake, Danjiangkou Reservoir, Yuncheng Salt Lake, and Nansi Lake was 28.75-47.27, 26.60-31.93, 33.56-39.30, and 27.22-43.01 ng·g-1, respectively. The toxic equivalents of high-cyclic PAHs in fish of the four lakes were significantly higher than those of low-cyclic and middle-cyclic PAHs (P < 0.05). In Taihu Lake, Danjiangkou Reservoir, and Nansi Lake, the toxicity equivalents were predominantly contributed by benzo[a] pyrene (BaP), while in Yuncheng Salt Lake, dibenzo(a,h) anthracene (DahA) was the main contributor. The residents in central and eastern China exposed to PAHs had a negligible non-cancer risk (non-carcinogenic risk values <1) and a potential low cancer risk. It was noteworthy that the Pleuronichthys cornutus and Lateolabrax japonicus from Yuncheng Salt Lake could pose carcinogenic risks (>10-4) to humans, with benzo[b]fluoranthene (BbF) having the highest risk contribution rate. Source analysis indicated that the main source of PAHs in fish was combustion sources. BaP, DahA, and BbF could become potential pollutants of concern in the field of ecotoxicology. The results of this study on PAHs bioaccumulation, pollution characteristics, sources and health risks in fish from four lakes would provide a scientific basis for local governments to formulate targeted environmental management policies, pollution control measures, and public health strategies.

Asunto(s)

Monitoreo del Ambiente , Peces , Lagos , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Hidrocarburos Policíclicos Aromáticos/análisis , Hidrocarburos Policíclicos Aromáticos/metabolismo , China , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/metabolismo , Lagos/química , Animales , Medición de Riesgo , Peces/metabolismo , Bioacumulación

RESUMEN

Polycyclic aromatic hydrocarbons (PAHs) are associated with micropores in sediments and soils. This limits the bioaccessibility of these compounds via existing bioremediation technologies, as biodegradation is strongly influenced by the ability of bacteria to access different sizes of pores. In this work, we employed naphthalene and pyrene as model contaminants to evaluate the transformation capacity of the soil bacterium Pseudomonas putida G7 (2 × 1 µm) via mineralization and co-metabolic activity, respectively. Under non-growing conditions and in the absence of hydraulic flow, we examined how the tactic behavior of this motile bacterium influenced biodegradation of these two PAHs when passing through membranes with micrometer-sized pores (3 and 5 µm). The bacteria were spontaneously retained by the membranes, which blocked the contaminants away from a passive dosing source. However, the cells were mobilized through 5 µm pores after the application plant root exudate components (γ-aminobutyric acid, citrate and fructose) as strong chemoeffectors, which enhanced the mineralization of naphthalene and co-metabolism of pyrene. The tactic-mediated biodegradation enhancement did not occur through 3 µm pores, possibly due a physical constrain to the gradient sensing mechanism. Our results suggest that bacterial transport by chemotaxis may enhance the biotransformation of poorly bioaccessible contaminants present in micro-meter scale environments.

Asunto(s)

Biodegradación Ambiental , Hidrocarburos Policíclicos Aromáticos , Contaminantes del Suelo , Contaminantes del Suelo/metabolismo , Hidrocarburos Policíclicos Aromáticos/metabolismo , Pseudomonas putida/metabolismo , Microbiología del Suelo , Naftalenos/metabolismo , Pirenos/metabolismo

RESUMEN

Mitigating the negative impacts of polycyclic aromatic hydrocarbons (PAHs) is an urgent need due to their toxicity and persistence in the environment. This study investigated the use of Pseudomonas aeruginosa ASU-B6 to detoxify pyrene (PY). The bacterium P. aeruginosa ASU-B6 is capable of degrading PY by 92% as a sole carbon source after 15 days of incubation with phthalate being the major metabolic product. In this regard, the impact of pyrene (PY), P. aeruginosa ASU-B6 (ASU-B6), the bacterial strain combined with pyrene (ASU-B6/PY) and the metabolites produced after pyrene degradation (PY-metabolites) on the germination and physiological attributes of Hordeum vulgare and Vicia faba seedlings were studied. A single application of PY or ASU-B6 showed a toxic effect on the germination of both tested seeds. Interestingly, broad bean seedlings exhibited less sensitivity to PY stress in terms of growth and metabolism compared to barley. Notably, ASU-B6 inhibited fresh and dry weight of shoots and roots of barley and, to a lesser extent, reduced the germination of broad beans compared to the control. However, the combined PY-metabolites and ASU-B6/PY showed a mutual ameliorative effect on seedlings growth, alleviating the phytotoxic impact of each component. Pyrene reduced the virulence of ASU-B6 by inhibiting the production of pyocyanin pigment, while bacteria ameliorated pyrene toxicity through its degradation. Heatmap and principal component analyses highlighted that increasing the contents of hydrogen peroxide, superoxide anion, hydroxyl radical, and lipid peroxidation positively correlated to the toxicity of PY or ASU-B6. However, improving the antioxidant system which buffers the oxidative stress induced by different combinations of PY and ASU-B6 enhanced the growth of germinated seedlings corresponding to PY or ASU-B6. This study reflected the role of ASU-B6 in ameliorating PY-phytotoxicity. In addition, the application of ASU-B6 strain is recommended as a prospective candidate for remediation of PAHs-contaminated environment with a positive impact on the plant growth and metabolic products.

Asunto(s)

Germinación , Hordeum , Pseudomonas aeruginosa , Pirenos , Vicia faba , Hordeum/efectos de los fármacos , Hordeum/microbiología , Hordeum/metabolismo , Hordeum/crecimiento & desarrollo , Vicia faba/efectos de los fármacos , Vicia faba/microbiología , Vicia faba/metabolismo , Vicia faba/crecimiento & desarrollo , Pirenos/toxicidad , Pirenos/metabolismo , Pseudomonas aeruginosa/efectos de los fármacos , Pseudomonas aeruginosa/metabolismo , Germinación/efectos de los fármacos , Plantones/efectos de los fármacos , Plantones/microbiología , Plantones/crecimiento & desarrollo , Plantones/metabolismo , Hidrocarburos Policíclicos Aromáticos/toxicidad , Hidrocarburos Policíclicos Aromáticos/metabolismo , Biodegradación Ambiental

RESUMEN

The combined pollution of polycyclic aromatic hydrocarbons (PAHs) and organic cadmium (Cd) in farmland soils, and the field controlling strategy need to be studied urgently. In this study, 5 PAHs, 5 Cd and 11 soil conditioners were selected to explore the co-exposure risk and remediation efficiency. Firstly, a significant combination Fl-alkylalkoxy cadmium was obtained using forward and reverse methods coupling variation coefficient methods (the combined pollution value was 0.173). Secondly, the interaction energy of microbial degradation / plant absorption of Fl under Cd stress, and microbial mineralization / plant absorption of alkylalkoxy cadmium under PAHs stress were characterized using factorial experimental design, molecular docking and molecular dynamics simulation. The combined pollution of alkylalkoxy cadmium and dialkyl cadmium, phenanthrene and Benzo [a] pyrene was significant (synergistic contribution rates were 17.58 % and 19.22 %, respectively). In addition, 6 soil conditioners with significant efficiency were selected to design Taguchi orthogonal experimental schemes, indicating the microbial degradation / mineralization and plant absorption were significantly effective (the maximum increase of remediation efficiency was 93.81 %) under the combinations (i.e., trratone, coumarol, fulvamic acid, potassium fertilizer and others, etc.). Finally, it was found that the soil conditioners affected the hydrophobic groups and forces, and the efficiency was proportional to the highest peak value and minimum distance in the RDF curve. This study identifies the risk characteristics of co-exposure of PAHs and Cd and screens effective soil conditioners, providing theoretical guidance for risk controlling.

Asunto(s)

Biodegradación Ambiental , Cadmio , Hidrocarburos Policíclicos Aromáticos , Microbiología del Suelo , Contaminantes del Suelo , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/química , Hidrocarburos Policíclicos Aromáticos/metabolismo , Hidrocarburos Policíclicos Aromáticos/química , Cadmio/metabolismo , Cadmio/química , Plantas/metabolismo , Simulación por Computador , Metales Pesados/metabolismo , Metales Pesados/química , Simulación del Acoplamiento Molecular

RESUMEN

Fish exposed to xenobiotics like petroleum-derived polycyclic aromatic hydrocarbons (PAHs) will immediately initiate detoxification systems through effective biotransformation reactions. Yet, there is a discrepancy between recognized metabolic pathways and the actual metabolites detected in fish following PAH exposure like oil pollution. To deepen our understanding of PAH detoxification, we conducted experiments exposing Atlantic haddock (Melanogrammus aeglefinus) to individual PAHs or complex oil mixtures. Bile extracts, analyzed by using an ion mobility quadrupole time-of-flight mass spectrometer, revealed novel metabolites associated with the mercapturic acid pathway. A dominant spectral feature recognized as PAH thiols set the basis for a screening strategy targeting (i) glutathione-, (ii) cysteinylglycine-, (iii) cysteine-, and (iv) mercapturic acid S-conjugates. Based on controlled single-exposure experiments, we constructed an interactive library of 33 metabolites originating from 8 PAHs (anthracene, phenanthrene, 1-methylphenanthrene, 1,4-dimethylphenanthrene, chrysene, benz[a]anthracene, benzo[a]pyrene, and dibenz[a,h]anthracene). By incorporation of the library in the analysis of samples from crude oil exposed fish, PAHs conjugated with glutathione and cysteinylglycine were uncovered. This qualitative study offers an exclusive glimpse into the rarely acknowledged mercapturic acid detoxification pathway in fish. Furthermore, this furnishes evidence that this metabolic pathway also succeeds for PAHs in complex pollution sources, a notable discovery not previously reported.

Asunto(s)

Acetilcisteína , Petróleo , Hidrocarburos Policíclicos Aromáticos , Petróleo/metabolismo , Animales , Hidrocarburos Policíclicos Aromáticos/metabolismo , Acetilcisteína/metabolismo , Contaminantes Químicos del Agua/metabolismo , Gadiformes/metabolismo

RESUMEN

Pseudomonas aeruginosa PR23 isolated from the hydrocarbon contaminated soil can tolerate and degrade mixture of polyaromatic hydrocarbons (PAHs) at an initial concentration of 1300 ppm. The degradation and intermediates formed were assessed by gas chromatography-mass spectrometry (GC-MS) analysis. The isolated strain was able to degrade 59.2% of the mixture of PAHs in 3 days and 71.6% by day 15. Effect of PAHs on protein expression in Pseudomonas aeruginosa PR23 was studied using nano LC-MS/MS. Thirty-six proteins showed a more than 2-fold increase in expression in the presence of mixture of PAHs. Out of these proteins, 7 proteins have been reported for their role in degradation of naphthalene, phenanthrene, and pyrene. The data revealed the presence of 16 proteins that were uniquely expressed in the presence of mixture of PAHs. A twin-arginine translocation signal peptide (Tat system), known for the transportation of folded proteins across the cell membrane, showed more than 8-fold increased expression in the presence of mixture of PAHs. These results indicate that the isolated strain adopts the conditions in the presence of mixture of PAHs by modulating its metabolic and physiological processes. These findings suggest that Pseudomonas aeruginosa PR23 may be a suitable candidate for use in the development of strategies for bioremediation of mixtures of PAHs.

Asunto(s)

Proteínas Bacterianas , Biodegradación Ambiental , Hidrocarburos Policíclicos Aromáticos , Pseudomonas aeruginosa , Microbiología del Suelo , Contaminantes del Suelo , Pseudomonas aeruginosa/metabolismo , Pseudomonas aeruginosa/genética , Hidrocarburos Policíclicos Aromáticos/metabolismo , Contaminantes del Suelo/metabolismo , Proteínas Bacterianas/metabolismo , Proteínas Bacterianas/genética , Cromatografía de Gases y Espectrometría de Masas , Fenantrenos/metabolismo , Espectrometría de Masas en Tándem , Naftalenos/metabolismo

RESUMEN

The study investigates the pollution characteristics of 16 priority PAHs, accumulated in copepods from a major fishing harbour and its adjacent coastal waters of Veraval, west coast of India. The total PAH accumulation is in the range of 922.16-27,807.49 ng g-1 dw, with the mean concentration of 5776.59 ng g-1 dw. High concentrations of PAHs were present in the copepod samples from inside the harbour. Notably, there was no significant correlation between the lipid content of copepods and the accumulation of PAHs. The molecular diagnostic ratio method (MDR) indicates that the PAH sources are petrogenic in origin, while principal component analysis (PCA) points to petroleum, coal combustion and vehicular emission sources. Total cancerous PAHs (C-PAHs) in the study area dominate by 40% of the total PAHs identified; moreover, the bioaccumulation factor (BAF) is very high in the offshore area, which is also a fishing ground. The global relevance and magnitude of the present study in the Veraval, one of the prime seafood exporting hubs in India, should be dealt with utmost avidity as the accumulation status of PAHs in the zooplankton has never been explored in the Indian coastal waters. Moreover, the current study gives the foremost data on the bioaccumulation status of PAHs in copepods from the tropical waters of India.

Asunto(s)

Copépodos , Monitoreo del Ambiente , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Copépodos/metabolismo , Hidrocarburos Policíclicos Aromáticos/metabolismo , Hidrocarburos Policíclicos Aromáticos/análisis , Animales , Contaminantes Químicos del Agua/metabolismo , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente/métodos , India , Bioacumulación , Agua de Mar/química

RESUMEN

Simultaneous biodegradation of multiple micropollutantslike polycyclic aromatic hydrocarbons (PAHs) and phthalates (PAEs) by microbial consortia remain unclear. Here, four distinct bacterial consortia capable of degrading PAHs and PAEs were domesticated from sludge and its composts. PAH-degrading consortium HS and PAE-degrading consortium EC2 displayed the highest degradation efficiencies for PAHs (37 %-99 %) and PAEs (98 %-99 %), respectively, being significantly higher than those of individual member strains. Consortia HS and EC2 could simultaneously degrade both PAHs and PAEs. Remarkably, a synthetic consortium Syn by co-culturing consortia HS and EC2 demonstrated proficient simultaneous biodegradation for both PAHs (65 %-98 %) and PAEs (91 %-97 %). These consortia changed their community structure with enriching pollutant-degrading genera and extracellular polymeric substance contents to promote simultaneous biodegradation of multiple pollutants. Moreover, consortium Syn significantly enhanced degradation of both PAHs and PAEs in soil and sludge. This study provides strong candidates for simultaneous bioremediation of complex polluted environments by PAHs and PAEs.

Asunto(s)

Biodegradación Ambiental , Consorcios Microbianos , Ácidos Ftálicos , Hidrocarburos Policíclicos Aromáticos , Aguas del Alcantarillado , Contaminantes del Suelo , Aguas del Alcantarillado/microbiología , Hidrocarburos Policíclicos Aromáticos/metabolismo , Contaminantes del Suelo/metabolismo , Ácidos Ftálicos/metabolismo , Bacterias/metabolismo , Microbiología del Suelo

RESUMEN

Microalgae cultures have emerged as a promising strategy in diverse areas, ranging from wastewater treatment to biofuel production, thus contributing to the search for carbon neutrality. These photosynthetic organisms can utilize the resources present in wastewater and fix atmospheric CO2 to produce biomass with high energy potential. In this study, the removal efficiency of Polycyclic Aromatic Hydrocarbons (PAHs), CO2 fixation and lipid content in the biomass produced from microalgae grown in airlift photobioreactor were evaluated. Four mesoscale cultures were carried out: Control (Seawater + Conway medium), Treatment A (Oil Produced Water + Poultry Effluent Water), Treatment B (Poultry Effluent Water + Seawater) and Treatment C (Oil Produced Water, Seawater and nutrients). The impact of biostimulation, through the addition of nutrients, on PAHs removal efficiency (up to 90%), CO2 fixation rate (up to 0.20 g L-1 d-1) and the composition of the generated biomass was observed. Primarily, the addition of nitrates to the culture medium impacted CO2 fixation rate of the microalgae. In addition, a direct correlation was observed between PAHs removal and lipid accumulation in the biomass, up to 36% in dry weight, demonstrating microalgae's ability to take advantage of the organic carbon (PAHs) present in the culture medium to generate lipid-rich biomass. The concentration of polysaccharides in the biomass obtained did not exceed 12% on a dry weight basis, and the Higher Heating Value (HHV) ranged between 17 and 21 MJ kg-1. Finally, the potential of generating hydrogen through pyrolysis was highlighted, taking advantage of the characteristics of biomass as a conversion route to produce biofuels. These results show that microalgae are effective in wastewater treatment and have great potential in producing biofuels, thus contributing to the transition towards more sustainable energy sources and climate change mitigation.

Asunto(s)

Biocombustibles , Dióxido de Carbono , Microalgas , Fotobiorreactores , Hidrocarburos Policíclicos Aromáticos , Aguas Residuales , Microalgas/metabolismo , Microalgas/crecimiento & desarrollo , Biocombustibles/análisis , Dióxido de Carbono/metabolismo , Dióxido de Carbono/análisis , Aguas Residuales/química , Aguas Residuales/microbiología , Hidrocarburos Policíclicos Aromáticos/metabolismo , Hidrocarburos Policíclicos Aromáticos/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/metabolismo , Biomasa , Eliminación de Residuos Líquidos/métodos

RESUMEN

Polycyclic aromatic hydrocarbons (PAHs) are a type of organic pollution that can accumulate in crops and hazard human health. This study used phenanthrene (PHE) as a model PAH and employed hydroponic experiments to illustrate the role of indole-3-acetic acid (IAA) in the regulation of PHE accumulation in wheat roots. At optimal concentrations, wheat roots treated with PHE + IAA showed a 46.9% increase in PHE concentration, whereas treatment with PHE + P-chlorophenoxyisobutyric acid resulted in a 38.77% reduction. Transcriptome analysis identified TaSAUR80-5A as the crucial gene for IAA-enhancing PHE uptake. IAA increases plasma membrane H+-ATPase activity, promoting active transport of PHE via the PHE/H+ cotransport mechanism. These results provide not only the theoretical basis necessary to better understand the function of IAA in PAHs uptake and transport by staple crops, but also a strategy for controlling PAHs accumulation in staple crops and enhancing phytoremediation of PAH-contaminated environments.

Asunto(s)

Biodegradación Ambiental , Ácidos Indolacéticos , Fenantrenos , Raíces de Plantas , Contaminantes del Suelo , Triticum , Fenantrenos/metabolismo , Triticum/metabolismo , Ácidos Indolacéticos/metabolismo , Raíces de Plantas/metabolismo , Contaminantes del Suelo/metabolismo , Transporte Biológico , Hidrocarburos Policíclicos Aromáticos/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/genética

RESUMEN

Sulfate-reducing bacteria (SRB) are crucial players in global biogeochemical cycling and some have been implicated in the anaerobic biodegradation of organic pollutants, including recalcitrant and hazardous polycyclic aromatic hydrocarbons (PAHs). Obtaining PAH-degrading SRB cultures for laboratories is of paramount importance in the development of the young field of anaerobic biodegradation of PAHs. SRB grow exceptionally slowly on PAH substrates and are highly sensitive to oxygen. Consequently, enrichment and maintenance of PAH-degrading SRB cultures and characterization of the biodegradation process remain a tedious and formidable task, especially for new researchers. To address these technical constraints, we have developed robust and effective protocols for obtaining and characterizing PAH-degrading SRB cultures. In this set of protocols, we describe step-by-step procedures for preparing inocula from contaminated soil or sediment, preparing anoxic medium, establishing enrichment cultures with PAHs as substrates under completely anaerobic sulfate-reducing conditions, successive culture transfers to obtain highly enriched cultures, rapid verification of the viability of SRB in slow-growing cultures, assessment of PAH degradation by extracting residuals using organic solvent and subsequent analysis by gas chromatography-mass spectrometry, and spectrophotometric determination of sulfate and sulfide in miniaturized, medium-throughput format. These protocols are expected to serve as a comprehensive manual for obtaining and characterizing PAH-degrading sulfate-reducing cultures. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Obtaining PAH-degrading strictly anaerobic sulfate-reducing enrichment cultures from contaminated soil and sediment Support Protocol 1: Operation and maintenance of an anaerobic workstation Support Protocol 2: Setup of gas purging systems for preparing anoxic solutions Support Protocol 3: Verification of viability in slow-growing SRB enrichment cultures Support Protocol 4: Extraction of genomic DNA from low-biomass cultures Basic Protocol 2: Extraction of residual PAH from liquid culture and analysis by GC-MS Basic Protocol 3: Spectrophotometric determination of sulfate concentration in SRB cultures Basic Protocol 4: Spectrophotometric determination of sulfide concentrations in SRB cultures by the methylene blue method Alternate Protocol: Spectrophotometric determination of sulfide concentrations in SRB cultures by the colloidal copper sulfide method.

Asunto(s)

Biodegradación Ambiental , Sedimentos Geológicos , Hidrocarburos Policíclicos Aromáticos , Sulfatos , Hidrocarburos Policíclicos Aromáticos/metabolismo , Sedimentos Geológicos/microbiología , Anaerobiosis , Sulfatos/metabolismo , Contaminantes del Suelo/metabolismo , Contaminantes del Suelo/análisis , Microbiología del Suelo , Cromatografía de Gases y Espectrometría de Masas

RESUMEN

Sediment, as the destination of marine pollutants, often bears much more serious petroleum pollution than water. Biochar is increasingly utilized for remediating organic pollutant-laden sediments, yet its long-term impacts on oil-contaminated sediment remain poorly understood. In this study, simulation experiments adding 2.5 wt% biochars (corn straw and wood chips biochar at different pyrolysis temperatures) were conducted. The effects on petroleum hydrocarbon attenuation, enzyme activities, and microbial community structure were systematically investigated. Results showed enhanced degradation of long-chain alkanes in certain biochar-treated groups. Biochar species and PAH characteristics together lead to the PAHs' attenuation, with low-temperature corn straw biochar facilitating the degradation of phenanthrene, fluorene, and chrysene. Initially, biochars reduced polyphenol oxidase activity but increased urease and dehydrogenase activities. However, there was a noticeable rise in polyphenol oxidase activity for a long time. Biochars influenced bacterial community succession and abundance, likely due to nutrient release stimulating microbial activity. The structural equations model (SEM) reveals that DON affected the enzyme activity by changing the microbial community and thus regulated the degradation of PAHs. These findings shed light on biochar's role in bacterial communities and petroleum hydrocarbon degradation over extended periods, potentially enhancing biochar-based remediation for petroleum-contaminated sediments.

Asunto(s)

Biodegradación Ambiental , Carbón Orgánico , Sedimentos Geológicos , Petróleo , Hidrocarburos Policíclicos Aromáticos , Carbón Orgánico/química , Sedimentos Geológicos/química , Sedimentos Geológicos/microbiología , Petróleo/metabolismo , Hidrocarburos Policíclicos Aromáticos/química , Hidrocarburos Policíclicos Aromáticos/metabolismo , Bacterias/metabolismo , Bacterias/efectos de los fármacos , Contaminación por Petróleo , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidad , Hidrocarburos/metabolismo , Hidrocarburos/química , Microbiota/efectos de los fármacos , Catecol Oxidasa/metabolismo

RESUMEN

It has been well-investigating that individual phthalates (PAEs) or polycyclic aromatic hydrocarbons (PAHs) affect public health. However, there is still a gap that the mixture of PAEs and PAHs impacts birth outcomes. Through innovative methods for mixtures in epidemiology, we used a metabolome Exposome-Wide Association Study (mExWAS) to evaluate and explain the association between exposure to PAEs and PAHs mixtures and birth outcomes. Exposure to a higher level of PAEs and PAHs mixture was associated with lower birth weight (maximum cumulative effect: 143.5 g) rather than gestational age. Mono(2-ethlyhexyl) phthalate (MEHP) (posterior inclusion probability, PIP = 0.51), 9-hydroxyphenanthrene (9-OHPHE) (PIP = 0.53), and 1-hydroxypyrene (1-OHPYR) (PIP = 0.28) were identified as the most important compounds in the mixture. In mExWAS, we successfully annotated four overlapping metabolites associated with both MEHP/9-OHPHE/1-OHPYR and birth weight, including arginine, stearamide, Arg-Gln, and valine. Moreover, several lipid-related metabolism pathways, including fatty acid biosynthesis and degradation, alpha-linolenic acid, and linoleic acid metabolism, were disturbed. In summary, these findings may provide new insights into the underlying mechanisms by which PAE and PAHs affect fetal growth.

Asunto(s)

Metaboloma , Ácidos Ftálicos , Hidrocarburos Policíclicos Aromáticos , Hidrocarburos Policíclicos Aromáticos/metabolismo , Ácidos Ftálicos/metabolismo , Humanos , Femenino , Embarazo , Metaboloma/efectos de los fármacos , Peso al Nacer/efectos de los fármacos , Exposoma , Contaminantes Ambientales/metabolismo , Exposición Materna/estadística & datos numéricos , Recién Nacido , Adulto

RESUMEN

The anaerobic biodegradation of polycyclic aromatic hydrocarbons (PAHs) is challenging due to its toxic effect on the microbes. Microbial electrolysis cells (MECs), with their excellent characteristics of anodic and cathodic biofilms, can be a viable way to enhance the biodegradation of PAHs. This work assessed different cathode materials (carbon brush and nickel foam) combined with bioaugmentation on typical PAHs-naphthalene biodegradation and analyzed the inhibition amendment mechanism of microbial biofilms in MECs. Compared with the control, the degradation efficiency of naphthalene with the nickel foam cathode supplied with bioaugmentation dosage realized a maximum removal rate of 94.5 ± 3.2%. The highest daily recovered methane yield (227 ± 2 mL/gCOD) was also found in the nickel foam cathode supplied with bioaugmentation. Moreover, the microbial analysis demonstrated the significant switch of predominant PAH-degrading microorganisms from Pseudomonas in control to norank_f_Prolixibacteraceae in MECs. Furthermore, hydrogentrophic methanogenesis prevailed in MEC reactors, which is responsible for methane production. This study proved that MEC combined with bioaugmentation could effectively alleviate the inhibition of PAH, with the nickel foam cathode obtaining the fastest recovery rate in terms of methane yield.

Asunto(s)

Biodegradación Ambiental , Electrólisis , Hidrocarburos Policíclicos Aromáticos , Aguas Residuales , Contaminantes Químicos del Agua , Hidrocarburos Policíclicos Aromáticos/metabolismo , Hidrocarburos Policíclicos Aromáticos/química , Aguas Residuales/química , Contaminantes Químicos del Agua/metabolismo , Contaminantes Químicos del Agua/química , Eliminación de Residuos Líquidos/métodos , Reactores Biológicos , Bacterias/metabolismo , Electrodos , Biopelículas

RESUMEN

Polycyclic aromatic hydrocarbons (PAHs) were frequently found in sediment and were primarily treated through microbial degradation. Thus, efficient management of PAH pollution requires exploring the molecular degradation mechanisms of PAHs and expanding the pool of available microbial resources. A fungus (identified as Sarocladium terricola strain RCEF778) with the remarkable ability to degrade pyrene was screened from sediment near a petrochemical plant, and its growth and pyrene degradation characteristics were comprehensively investigated. The results showed that the fungus exhibited great effectiveness in pyrene degradation, with a degradation ratio of 88.97% at 21 days at the conditions: 35 °C, pH 7, 10 mg L-1 initially pyrene concentration, 3% supplementary salt, and glucose supplementation. The generation and concentration variation of the intermediate products were identified, and the results revealed that the fungus degraded pyrene through two pathways: by salicylic acid and by phthalic acid. Three sediments (M1, M2, M3), each exhibiting different levels of PAH pollution, were employed to examine the effectiveness of fungal degradation of PAHs in practical sediment samples. These data showed that with the fungus, the degradation ratios ranged from 13.64% to 23.50% for 2-3 rings PAHs, 40.93%-49.41% for 4 rings PAHs, and 39.59%-48.07% for 5-6 rings PAHs, which were significantly higher than those for the sediment without the fungus and confirmed the excellent performance of the fungal. Moreover, the Gompertz model was employed to analyze the degradation kinetics of 4-rings and 5-6 rings PAHs in these sediments, and the results demonstrated that the addition of the fungus could significantly increase the maximum degradation ratio, degradation start-up rate and maximum degradation rate of 4-rings and 5-6 rings PAHs and shorten the time required to reach the maximum degradation rate. This study not only supplied fungal materials but also established crucial theoretical foundations for the development of bioremediation technologies aimed at high molecular weight PAH-contaminated sediments.

Asunto(s)

Biodegradación Ambiental , Sedimentos Geológicos , Hidrocarburos Policíclicos Aromáticos , Hidrocarburos Policíclicos Aromáticos/metabolismo , Sedimentos Geológicos/microbiología , Pirenos/metabolismo

RESUMEN

Environmental contaminants, such as polycyclic aromatic hydrocarbons (PAHs), have raised concerns regarding their potential endocrine-disrupting effects on aquatic organisms, including fish. In this study, molecular docking and molecular dynamics techniques were employed to evaluate the endocrine-disrupting potential of PAHs in zebrafish, as a model organism. A virtual screening with 72 PAHs revealed a correlation between the number of PAH aromatic rings and their binding affinity to proteins involved in endocrine regulation. Furthermore, PAHs with the highest binding affinities for each protein were identified: cyclopenta[cd]pyrene for AR (-9.7 kcal/mol), benzo(g)chrysene for ERα (-11.5 kcal/mol), dibenzo(a,e)pyrene for SHBG (-8.7 kcal/mol), dibenz(a,h)anthracene for StAR (-11.2 kcal/mol), and 2,3-benzofluorene for TRα (-9.8 kcal/mol). Molecular dynamics simulations confirmed the stability of the protein-ligand complexes formed by the PAHs with the highest binding affinities throughout the simulations. Additionally, the effectiveness of the protocol used in this study was demonstrated by the receiver operating characteristic curve (ROC) analysis, which effectively distinguished decoys from true ligands. Therefore, this research provides valuable insights into the endocrine-disrupting potential of PAHs in fish, highlighting the importance of assessing their impact on aquatic ecosystems.

Asunto(s)

Disruptores Endocrinos , Simulación del Acoplamiento Molecular , Simulación de Dinámica Molecular , Hidrocarburos Policíclicos Aromáticos , Pez Cebra , Hidrocarburos Policíclicos Aromáticos/química , Hidrocarburos Policíclicos Aromáticos/metabolismo , Hidrocarburos Policíclicos Aromáticos/toxicidad , Animales , Disruptores Endocrinos/química , Disruptores Endocrinos/metabolismo , Disruptores Endocrinos/toxicidad , Unión Proteica , Sitios de Unión , Proteínas de Pez Cebra/metabolismo , Proteínas de Pez Cebra/química , Ligandos , Curva ROC , Contaminantes Químicos del Agua/metabolismo , Contaminantes Químicos del Agua/química , Contaminantes Químicos del Agua/toxicidad , Receptor alfa de Estrógeno/metabolismo , Receptor alfa de Estrógeno/química