RESUMEN
Several smokeless tobacco products are available in the market and comprise complex chemical matrices. Sample preparation for analysis of the multiple classes of harmful compounds in smokeless tobacco products is highly cumbersome. In this study, a simultaneous extraction scheme was developed for three toxic analyte classes in smokeless tobacco products using a two-phase solution consisting of 5% aqueous NaOH and dichloromethane in a 1:4 ratio. The dichloromethane extract was used to analyze four alkaloids directly at levels greater than parts per million; however, passing the layer through a silica cartridge for further purification and concentration was necessary for determining 18 polycyclic aromatic hydrocarbons and four volatile N-nitrosoamines at the ppt level. The multitargets were determined by using gas chromatography with tandem mass spectrometry. The limits of detection for the 18 polycyclic aromatic hydrocarbons, four volatile N-nitrosoamines, three minor alkaloids, and nicotine were 0.2-1.2, 0.2-0.4, 0.6-1.0, and 10.2 µg/g, respectively. Four different smokeless tobacco substrates were fortified with three levels of mixed standards, and the recoveries ranged between 83 and 110%. The method was highly efficient, reduced the sample amounts, solvents, and the time required by approximately 60%. The method was used to assay 18 smokeless tobacco products, and showed potentials in assaying drugs and other plant-based substrates.
Asunto(s)
Alcaloides/análisis , Hidrocarburos Cíclicos/análisis , Nitrosaminas/análisis , Tabaco sin Humo/análisis , Cromatografía de Gases y Espectrometría de Masas/métodos , Espectrometría de Masas en Tándem/métodosRESUMEN
Most of the shellfish fisheries of Mexico occur in the Gulf of California. In this region, known for its high primary productivity, blooms of diatoms and dinoflagellates are common, occurring mainly during upwelling events. Dinoflagellates that produce lipophilic toxins are present, where some outbreaks related to okadaic acid and dinophisystoxins have been recorded. From January 2015 to November 2017 samples of three species of wild bivalve mollusks were collected monthly in five sites in the southern region of Bahía de La Paz. Pooled tissue extracts were analyzed using LC-MS/MS to detect lipophilic toxins. Eighteen analogs of seven toxin groups, including cyclic imines were identified, fortunately individual toxins did not exceed regulatory levels and also the total toxin concentration for each bivalve species was lower than the maximum permitted level for human consumption. Interspecific differences in toxin number and concentration were observed in three species of bivalves even when the samples were collected at the same site. Okadaic acid was detected in low concentrations, while yessotoxins and gymnodimines had the highest concentrations in bivalve tissues. Although in low quantities, the presence of cyclic imines and other lipophilic toxins in bivalves from the southern Gulf of California was constant.
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Bivalvos/metabolismo , Toxinas Marinas/análisis , Animales , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Toxinas Marinas/química , Venenos de Moluscos , Ácido Ocadaico/análisis , Oxocinas/análisis , SolubilidadRESUMEN
BACKGROUND: The manila clam Ruditapes philippinarum and the wedge clam Donax trunculus are economic bivalve species which constitute an important part of the natural bivalve beds in the Marmara Sea, Turkey. Toxic chemicals such as, dioxins, dioxin-like polychlorinated biphenyls (PCBs), polycyclic hydrocarbons (PAHs), and toxic metals are recognized as important health risk factors that threaten public health via food or directly in the environment. In the present study, we aimed to determine and monitor levels of these toxic chemicals in both clam species, R. philippinarum and D. trunculus, between 2013 and 2017. RESULTS: According to the results, maximum levels of dioxins, PAHs, and toxic metals (lead, cadmium, mercury) during the period were determined as 0.18 pg g-1 , 2.43 mg kg-1 , and 0.44-0.53-0.1 mg kg-1 , respectively. The level of contaminants in both clam species were determined to be below the threshold or tolerable daily intake values established by the European Commission, European Food Safety Authority (EFSA), and Food and Agriculture Organization/World Health Organization (FAO/WHO). Seasonal fluctuations were observed to be similar in both species and also in seasons, but elevated levels of PCBs and PAHs were detected in warmer months during the period. CONCLUSION: As a consequence, toxic chemicals in both clam species were found at low concentrations in this study from the point of view of public health concerns; however, the contaminants should be closely monitored in the future due to their elevated levels in samples. © 2020 Society of Chemical Industry.
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Bivalvos/química , Hidrocarburos Cíclicos/análisis , Metales Pesados/análisis , Bifenilos Policlorados/análisis , Agua de Mar/análisis , Mariscos/análisis , Contaminantes Químicos del Agua/análisis , Animales , Contaminación de Alimentos/análisis , TurquíaRESUMEN
The first survey of the phycotoxin profile in mussels (Mytilus galloprovincialis) from the coastal waters of Bosnia and Herzegovina (The Bay of Neum, Middle Adriatic Sea) in correlation to the Makarska City Bay (Croatia, Middle Adriatic Sea) was conducted in 2017. Throughout the monitoring period, occasions of gymnodimine (GYM) and azaspiracid (AZA2) shellfish toxicity were recorded in concentrations that do not endanger human health. The occurrence of yessotoxins (YTXs), the most common toxins found in the Adriatic Sea, was correlated to the presence of the Gonyaulax species, a potential source of YTX. The DSP group of toxins is represented by the ester-OA. Phytoplankton analysis confirmed the presence of dinoflagellates from the Prorocentrum genus, a species associated with DSP toxicity. Occurrence frequency and variability of toxin composition were investigated in conjunction to physico-chemical parameters in the surrounding sea water. In the central Adriatic Sea, the infestation period ranges in general from June to August. However, the depuration phase extended beyond September in the Bay of Neum, increasing the length of the decontamination period.
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Toxinas Marinas/análisis , Venenos de Moluscos/análisis , Mariscos/estadística & datos numéricos , Animales , Croacia , Dinoflagelados , Compuestos Heterocíclicos con 3 Anillos/análisis , Humanos , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Mytilus , Oxocinas/análisis , Fitoplancton , Alimentos Marinos , Intoxicación por Mariscos , Compuestos de Espiro/análisisRESUMEN
Marine algal toxins, highly toxic secondary metabolites, have significant influences on coastal ecosystem health and mariculture safety. The occurrence and environmental control factors of lipophilic marine algal toxins (LMATs) in the surface seawater of the Changjiang estuary (CJE) and the adjacent East China Sea (ECS) were investigated. Pectenotoxin-2 (PTX2), okadaic acid (OA), dinophysistoxin-1(DTX1), and gymnodimine (GYM) were detected in the CJE surface seawater in summer, with concentration ranges of not detected (ND)-105.54 ng/L, ND-13.24 ng/L, ND-5.48 ng/L, and ND-12.95 ng/L, respectively. DTX1 (ND-316.15 ng/L), OA (ND-16.13 ng/L), and PTX2 (ND-4.97 ng/L) were detected in the ECS during spring. LMATs formed a unique low-concentration band in the Changjiang diluted water (CJDW) coverage area in the typical large river estuary. PTX2, OA, and DTX1 in seawater were mainly derived from Dinophysis caudate and Dinophysis rotundata, while GYM was suspected to be from Karenia selliformis. Correlation analyses showed that LMAT levels in seawater were positively correlated with dissolved oxygen and salinity, but negatively correlated with temperature and nutrients, indicating that the hydrological condition and nutritional status of seawater and climatic factors exert significant effects on the distribution of LMATs.
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Furanos/análisis , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Toxinas Marinas/análisis , Ácido Ocadaico/análisis , Piranos/análisis , Contaminantes del Agua/análisis , China , Dinoflagelados/química , Monitoreo del Ambiente , Estuarios , Macrólidos , Océanos y Mares , Fitoplancton/química , Agua de Mar/análisisRESUMEN
Lipophilic marine toxins in shellfish pose significant threats to the health of seafood consumers. To assess the contamination status of shellfish by lipophilic marine toxins in the Bohai Sea, nine species of shellfish periodically collected from five representative aquaculture zones throughout a year were analyzed with a method of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Lipophilic marine toxins, including okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), yessotoxin (YTX), homo-yessotoxin (homo-YTX), azaspiracids (AZA2 and AZA3), gymnodimine (GYM), and 13-desmethyl spirolide C (13-DesMe-C), were detected in more than 95 percent of the shellfish samples. Toxins PTX2, YTX, 13-DesMe-C and GYM were predominant components detected in shellfish samples. Scallops, clams and mussels accumulated much higher level of lipophilic marine toxins compared to oysters. Toxin content in shellfish samples collected from different sampling locations showed site-specific seasonal variation patterns. High level of toxins was found during the stages from December to February and June to July in Hangu, while from March to April and August to September in Laishan. Some toxic algae, including Dinophysis acuminata, D. fortii, Prorocentrum lima, Gonyaulax spinifera and Lingulodinium polyedrum, were identified as potential origins of lipophilic marine toxins in the Bohai Sea. The results will offer a sound basis for monitoring marine toxins and protecting the health of seafood consumers.
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Toxinas Marinas/análisis , Mariscos/análisis , Contaminantes Químicos del Agua/análisis , Animales , Bivalvos/química , China , Cromatografía Liquida/métodos , Dinoflagelados , Furanos/análisis , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Macrólidos , Venenos de Moluscos , Ácido Ocadaico/análisis , Ostreidae/química , Oxocinas/análisis , Piranos/análisis , Alimentos Marinos/análisis , Mariscos/estadística & datos numéricos , Compuestos de Espiro/análisis , Espectrometría de Masas en TándemRESUMEN
The risk of rheumatoid arthritis (RA) is linked to environmental and genetic factors. Cigarette smoking is an established environmental risk factor for the disease that contributes to its development and severity. Previously, we found that cigarette smoke condensate (CSC), both mainstream and sidestream, aggravates collagen type II-induced arthritis (CIA), which was observed following either intraperitoneal inoculation or nasal exposure. In the present study, we aimed to identify the compound in CSC, which aggravates CIA. By sequential fractionation and analysis, extraction with water/ether in different pH values, silica gel column chromatography, TLC, octadecyl silica (ODS) HPLC, GC/MS, and NMR, the active compound was identified as 5-hydroxy-2-methylpyridine (5H2MP). Its isomer 2-hydroxy-3-methylpyridine, but not 3-hydroxy-2-methylpyridine, was also active. 5H2MP was not mutagenic, and did not exhibit aryl hydrocarbon receptor-dependent activity. Our data help clarify the mechanism underlying the pathogenic effects of cigarette smoking on RA.
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Artritis Experimental/patología , Nicotiana , Piridinas/toxicidad , Humo , Animales , Línea Celular , Fraccionamiento Químico , Humanos , Hidrocarburos Cíclicos/análisis , Masculino , Ratones Endogámicos DBA , Pruebas de Mutagenicidad , Salmonella enterica/efectos de los fármacos , Salmonella enterica/genética , Humo/efectos adversos , Humo/análisisRESUMEN
This study evaluated the applicability of eight types of biomarkers namely, adamantanes, diamantanes, sesquiterpanes, steranes, terpanes, TA-steranes, MA-steranes, and alkylated PAHs, to characterize chemically dispersed oil (CDO) after the 60-day weathering. The stability of diagnostic ratios of the selected biomarkers was evaluated and summarized. The results indicated that the concentrations of biomarkers with low molecular weight, such as adamantanes, diamantanes, and sesquiterpanes, in CDO were markedly affected by weathering and the associated diagnostic ratios were changed extensively. Most of the alkylated PAHs were degraded during weathering as well. These biomarkers thus were not recommended for characterizing CDO. The majority of the terpanes, steranes, TA-steranes, and MA-steranes could be used for weathered CDO fingerprinting due to the relatively stable diagnostic ratios. The findings could help to identify applicable biomarkers for fingerprinting of weathered dispersed oil.
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Biomarcadores/análisis , Monitoreo del Ambiente/métodos , Hidrocarburos Cíclicos/análisis , Contaminación por Petróleo/análisisRESUMEN
The occurrence and seasonal variations of marine algal toxins in phytoplankton and oyster samples in Daya Bay (DYB), South China Sea were investigated. Two Dinophysis species, namely, D. caudata and D. acuminata complex, were identified as Okadaic acid (OA)/pectenotoxin (PTX) related species. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis demonstrated that 2.04-14.47 pg PTX2 per cell was the predominant toxin in single-cell isolates of D. caudata. D. acuminata was not subjected to toxin analysis. The occurrence of OAs in phytoplankton concentrates of net-haul sample coincided with the presence of D. accuminata complex, suggesting that this species is most likely an OA producer in this sea area. OA, dinophysistoxins-1 (DTX1), PTX2, PTX2sa, gymnodimine (GYM), homoyessotoxin (homoYTX), and domoic acid (DA) demonstrated positive results in net haul samples. To our best knowledge, this paper is the first to report the detection of GYM, DA, and homoYTX in phytoplankton samples in Chinese coastal waters. Among the algal toxins, GYM demonstrated the highest frequency of positive detections in phytoplankton concentrates (13/17). Five compounds of algal toxins, including OA, DTX1, PTX2, PTX2sa, and GYM, were detected in oyster samples. DA and homoYTX were not detected in oysters despite of positive detections for both in the phytoplankton concentrates. However, neither the presence nor absence of DA in oysters can be determined because extraction conditions with 100% methanol used to isolate toxins from oysters (recommended by the EU-Harmonised Standard Operating Procedure, 2015) would likely be unsuitable for this water-soluble toxin. In addition, transformation of DA during the digestion process of oysters may also be involved in the negative detections of this toxin. GYM exhibited the highest frequency of positive results in oysters (14/17). OAs were only detected in the hydrolyzed oyster samples. The detection rates of PTX and PTX2sa in oysters were lower than those in the net haul samples.
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Bahías/química , Dinoflagelados/metabolismo , Monitoreo del Ambiente/métodos , Toxinas Marinas/análisis , Ostreidae/química , Fitoplancton/química , Contaminantes Químicos del Agua/análisis , Animales , China , Cromatografía Liquida/métodos , Compuestos Heterocíclicos con 3 Anillos/análisis , Compuestos Heterocíclicos con 3 Anillos/metabolismo , Hidrocarburos Cíclicos/análisis , Hidrocarburos Cíclicos/metabolismo , Iminas/análisis , Iminas/metabolismo , Ácido Kaínico/análogos & derivados , Ácido Kaínico/análisis , Ácido Kaínico/metabolismo , Toxinas Marinas/metabolismo , Ácido Ocadaico/análisis , Ácido Ocadaico/metabolismo , Ostreidae/metabolismo , Fitoplancton/metabolismo , Piranos/análisis , Piranos/metabolismo , Espectrometría de Masas en Tándem , Contaminantes Químicos del Agua/metabolismoRESUMEN
Some dinoflagellates can produce lipophilic marine toxins, which pose potent threats to seafood consumers. In the Bohai Sea, an important semi-closed inland sea with intensive mariculture industry in China, there is little knowledge concerning lipophilic marine toxins and their potential threats. In this study, net-concentrated phytoplankton samples were periodically collected from 5 typical mariculture zones around the Bohai Sea, including Laishan (LS), Laizhou (LZ), Hangu (HG), Qinhuangdao (QHD) and Huludao (HLD) in 2013 and 2014, and a method using high performance liquid chromatography (HPLC) coupled with a Q-Trap mass spectrometer was applied to analyze seven representative lipophilic marine toxins, including okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), yessotoxin (YTX), azaspiracid-1 (AZA1), gymnodimine (GYM), and 13-desmethyl spirolide C (desMeC). The method had high sensitivity and repeatability, and exhibited satisfactory recoveries for most of the lipophilic marine toxins (92.1-108%) except for AZA1 (65.8-68.9%). Nearly all the lipophilic marine toxins could be detected in phytoplankton samples from the Bohai Sea. OA, DTX1 and PTX2 were predominant components and present in most of the phytoplankton samples. The maximum content of lipophilic marine toxin in phytoplankton samples concentrated from seawater (OA 464 pg L-1; DTX1 783 pg L-1; YTX 86.6 pg L-1; desMeC 15.6 pg L-1; PTX2 1.11 × 103 pg L-1) appeared in June 2014. Based on toxins present in phytoplankton samples, it is implied that seafood in the Bohai Sea is more likely to be contaminated by OA group and PTX group toxins, and spring is the high-risk season for toxin contamination.
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Dinoflagelados/química , Toxinas Marinas/análisis , Fitoplancton/química , Alimentos Marinos/normas , Animales , China , Cromatografía Líquida de Alta Presión/métodos , Furanos/análisis , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Interacciones Hidrofóbicas e Hidrofílicas , Iminas/análisis , Macrólidos , Venenos de Moluscos , Ácido Ocadaico/análisis , Oxocinas/análisis , Piranos/análisis , Alimentos Marinos/análisis , Compuestos de Espiro/análisis , Espectrometría de Masas en TándemRESUMEN
We investigated an epidemic mortality cluster of yellow-eyed penguins (Megadyptes antipodes) that involved 67 moribund or dead birds found on various beaches of the Otago Peninsula, New Zealand, between 21 January and 20 March 2013. Twenty-four carcases were examined post-mortem. Histological lesions of pulmonary, hepatic and splenic erythrophagocytosis and haemosiderosis were found in 23 of 24 birds. Fifteen birds also had haemoglobin-like protein droplets within renal tubular epithelial cells. Despite consistent histological lesions, a cause of death could not be established. Virology, bacteriology and molecular tests for avian influenza, avian paramyxovirus-1, avipoxvirus, Chlamydia psittaci, Plasmodium spp., Babesia spp., Leucocytozoon spp. and Toxoplasma gondii were negative. Tissue concentrations of a range of heavy metals (n = 4 birds) were consistent with low level exposure, while examination of proventricular contents and mucus failed to detect any marine biotoxins or Clostridium botulinum toxin. Hepatic concentrations of total polycyclic aromatic hydrocarbons (PAHs) (n = 5 birds) were similar to background concentrations of polycyclic aromatic hydrocarbons previously found in yellow-eyed penguins from the South Island of New Zealand, but there were significantly higher concentrations of 1-methylnapthelene and 2-methylnapthelene in the birds found dead in this mortality cluster. The biological significance of this finding is unclear. A temporal investigation of the epidemic did not indicate either a common source or propagative epidemic pattern. Although our investigation did not definitively implicate a toxic or infectious agent, we could not rule out causes such as toxic marine organisms or mycoplasmosis. Further investigations should therefore by carried out in the event of future mortality clusters.
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Enfermedades de las Aves/epidemiología , Spheniscidae , Animales , Enfermedades de las Aves/diagnóstico , Enfermedades de las Aves/mortalidad , Enfermedades de las Aves/patología , Especies en Peligro de Extinción , Femenino , Hidrocarburos Cíclicos/análisis , Hígado/metabolismo , Masculino , Metales Pesados/análisis , Ratones , Nueva Zelanda/epidemiología , Proventrículo , Análisis Espacio-TemporalRESUMEN
Based on a homemade device, gas purge microsyringe extraction (GP-MSE) of crude oil samples was developed for the first time. As a simple, fast, low-cost, sensitive and solvent-saving technique, GP-MSE provides some outstanding advantages over the widely used sample preparation methods for crude oils such as column chromatography (ASTM D2549). Several parameters affecting extraction efficiency were optimized, including extraction temperature, extraction time, extraction solvent, condensing temperature and purge gas flow rate. With the optimized GP-MSE conditions, several real crude oil samples were extracted, and trace diamondoids were determined using comprehensive two dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS). In total, more than 100 diamondoids were detected and 27 marker compounds were identified and quantified accurately. The limits of detection (LODs, S/N=3) were less than 0.08µg/L for all diamondoids. The relative standard deviation (RSD) was below 8%, ranging from 1.1 to 7.6%. The linearity of the developed method was in the range of 0.5-100.0µg/L with correlation coefficients (R2) more than 0.996. The recoveries obtained at spiking 50µg/L were between 81 and 108% for diamondoids in crude oil samples. The developed method can also be extended to the analysis of other components in crude oils and other complex matrices.
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Técnicas de Química Analítica/métodos , Cromatografía de Gases y Espectrometría de Masas , Hidrocarburos Cíclicos/análisis , Petróleo/análisis , Gases/química , Límite de DetecciónRESUMEN
To investigate the prevalence of lipophilic marine biotoxins in shellfish from the Chinese market, we used hydrophilic interaction liquid chromatography-tandem mass spectrometry (LC-MS/MS) to measure levels of okadaic acid (OA), azaspiracid (AZA1), pectenotoxin (PTX2), gymnodimine (GYM), and spirolide (SPX1). We collected and analyzed 291 shellfish samples from main production sites along a wide latitudinal transect along the Chinese coastline from December 2008 to December 2009. Results revealed a patchy distribution of the five toxins and highlighted the specific geographical distribution and seasonal and species variation of the putative toxigenic organisms. All five lipophilic marine biotoxins were found in shellfish samples. The highest concentrations of OA, AZA1, PTX2, GYM, and SPX1 were 37.3, 5.90, 16.4, 14.4, and 8.97 µg/kg, respectively. These values were much lower than the legislation limits for lipophilic shellfish toxins. However, the value might be significantly underestimated for the limited detection toxins. Also, these toxins were found in most coastal areas of China and were present in almost all seasons of the year. Thus, these five toxins represent a potential threat to human health. Consequently, studies should be conducted and measures should be taken to ensure the safety of the harvested product.
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Toxinas Marinas/análisis , Mariscos/análisis , Animales , Bivalvos/química , China , Cromatografía Liquida/métodos , Furanos/análisis , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Macrólidos , Ácido Ocadaico/análisis , Ostreidae/química , Pectinidae/química , Piranos/análisis , Mariscos/toxicidad , Compuestos de Espiro/análisis , Espectrometría de Masas en Tándem/métodosRESUMEN
We reported previously that okadaic acid (OA) and dinophysistoxin-1 (DTX1) were responsible for diarrhetic shellfish poisoning (DSP) incidents due to consuming cultivated mussels (Mytilus galloprovincialis) in coastal cities near the East China Sea in May 2011. Pectenotoxin-2 (PTX2) and its seco acids were also present in these mussels. Causative species of microalgae were not identified because detailed information on the location of the contaminated shellfish was not recorded. In order to explore potential causes for these poisoning events, the lipophilic toxin profiles in picked cells of Dinophysis and in shellfish samples collected from two mariculture zones in the East China Sea were analyzed in the present study. Single-cell isolates (100 cells total for each location) of Dinophysis were collected from the aquaculture zones of Gouqi Island (Ningbo City, Zhejiang Province) and Qingchuan Bay (Ningde City, Fujian Province) in July and September 2013, respectively, for lipophilic toxin profiling. Shellfish samples collected over the course of a year from the Gouqi Island aquaculture zone and mussels (M. galloprovincialis) collected four times from the Qingchuan Bay aquaculture zone were tested for lipophilic toxins by LC-MS/MS. The Dinophysis cells isolated from both sampling sites were identified under the light microscope as Dinophysis caudata. Average quota of PTX2, the predominant toxin in D. caudata isolated from the coastal waters of Gouqi Island and Qingchuan Bay, was 0.58 and 2.8 pg/cell, respectively. Only trace amounts of OA and DTX1 were detected in D. caudata. PTX2, PTX2sa, 7-epi-PTX2sa, OA, and/or DTX1 were found in samples of mussels (M. galloprovincialis and Mytilus coruscus) collected in the Gouqi Island aquaculture zone from the end of May to the beginning of July 2013. PTX2, PTX2sa, and 7-epi-PTX2sa were also detected in oyster (Crassostrea gigas) during that period, but almost no OA and DTX1 were present. Gymnodimine (GYM) was detected in almost all mussel (M. coruscus) samples, with the highest levels occurring in winter. Trace amounts of pectenotoxins (PTXs) and OAs were also found in mussels (M. galloprovincialis) collected from Qingchuan Bay. D. caudata is suggested as an important source of PTXs in shellfish cultivated in the East China Sea. This is the first report of toxin profiles for single-cell isolates of Dinophysis in the East China Sea.
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Acuicultura/estadística & datos numéricos , Bivalvos/química , Dinoflagelados/química , Toxinas Marinas/análisis , Mariscos/análisis , Animales , China , Cromatografía Liquida , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Océanos y Mares , Espectrometría de Masas en TándemRESUMEN
To promote possible applications of graphene in molecular identification based on stacking effects, in particular in recognizing aromatic amino acids and even sequencing nucleobases in life sciences, we comprehensively study the interaction between graphene segments and different cyclic organic hydrocarbons including benzene (C6H6), cyclohexane (C6H12), benzyne (C6H4), cyclohexene (C6H10), 1,3-cyclohexadiene (C6H8(1)) and 1,4-cyclohexadiene (C6H8(2)), using the density-functional tight-binding (DFTB) method. Interestingly, we find obviously different characteristics in Raman vibrational and ultraviolet visible absorption spectra of the small molecules adsorbed on the graphene sheet. Specifically, we find that both spectra involve clearly different characteristic peaks, belonging to the different small molecules upon adsorption, with the ones of ionized molecules being more substantial. Further analysis shows that the adsorptions are almost all due to the presence of dispersion energy in neutral cases and involve charge transfer from the graphene to the small molecules. In contrast, the main binding force in the ionic adsorption systems is the electronic interaction. The results present clear signatures that can be used to recognize different kinds of aromatic hydrocarbon rings on graphene sheets. We expect that our findings will be helpful for designing molecular recognition devices using graphene.
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Grafito/química , Hidrocarburos Cíclicos/análisis , Hidrocarburos Cíclicos/metabolismo , Absorción , Biopolímeros/química , Biopolímeros/metabolismo , Ciclohexenos/química , Ciclohexenos/metabolismo , Hidrocarburos Cíclicos/química , Enlace de Hidrógeno , Modelos Químicos , Espectrofotometría Ultravioleta , Propiedades de Superficie , VibraciónRESUMEN
RATIONALE: Gymnodimines (GYMs) are fast-acting toxins that belong to the cyclic imine group, a subclass of lipophilic marine toxins. GYMs are considered to be emerging toxins but have not yet been linked to incidents of human poisoning, Limited knowledge on the metabolism of GYMs means that a proper risk assessment has not been possible and caution must be taken when establishing the relevance of GYMs in terms of food safety of marine products. METHODS: A series of mass spectrometric experiments involving precursor and product ion scans, selected reaction monitoring (SRM), and high-resolution mass spectrometry (MS) were used to detect and confirm 10-O-acyl esters of gymnodimine-A (1). RESULTS: We have detected for the first time the presence of a range of acyl ester derivatives of GYMs in shellfish samples from the Gulf of Gabes, Tunisia. The MS fragmentation pathways of 1 and its esters were also elucidated. Partial synthesis of a palmitic acid ester of 1 facilitated confirmation of identity and calibration of SRM analyses. Evidence of acyl ester metabolites of gymnodimine-B and -C was also obtained. CONCLUSIONS: A semi-quantitative analysis indicated that the majority of GYMs present in the sample were in the acylated form (>90%), suggesting that these compounds must not be neglected when trying to understand the risks associated with GYMs. There is a clear need for toxicology studies on these esters and assessment of bio-availability to humans.
Asunto(s)
Bivalvos/química , Cromatografía Liquida/métodos , Ácidos Grasos/análisis , Compuestos Heterocíclicos con 3 Anillos/análisis , Hidrocarburos Cíclicos/análisis , Iminas/análisis , Espectrometría de Masas/métodos , Mariscos/análisis , Animales , Bivalvos/metabolismo , Ácidos Grasos/química , Compuestos Heterocíclicos con 3 Anillos/química , Hidrocarburos Cíclicos/química , Iminas/química , Toxinas Marinas , TúnezRESUMEN
Biologically active macrocycles containing a cyclic imine were isolated for the first time from aquaculture sites in Nova Scotia, Canada, during the 1990s. These compounds display a "fast-acting" toxicity in the traditional mouse bioassay for lipophilic marine toxins. Our work aimed at developing a receptor-based detection method for spirolides using a microsphere/flow cytometry Luminex system. For the assay, two alternatives were considered as binding proteins, the Torpedo marmorata nicotinic acetylcholine receptor (nAChR) and the Lymnaea stagnalis acetylcholine binding protein (Ls-AChBP). A receptor-based inhibition assay was developed using the immobilization of nAChR or Ls-AChBP on the surface of carboxylated microspheres and the competition of cyclic imines with biotin-α-bungarotoxin (α-BTX) for binding to these proteins. The amount of biotin-α-BTX bound to the surface of the microspheres was quantified using phycoerythrin (PE)-labeled streptavidin, and the fluorescence was analyzed in a Luminex 200 system. AChBP and nAChR bound to 13-desmethyl spirolide C efficiently; however, the cross-reactivity profile of the nAChR for spirolides and gymnodimine more closely matched the relative toxic potencies reported for these toxins. The nAChR was selected for further assay development. A simple sample preparation protocol consisting of an extraction with acetone yielded a final extract with no matrix interference on the nAChR/microsphere-based assay for mussels, scallops, and clams. This cyclic imine detection method allowed the detection of 13-desmethyl spirolide C in the range of 10-6000 µg/kg of shellfish meat, displaying a higher sensitivity and wider dynamic range than other receptor-based assays previously published. This microsphere-based assay provides a rapid, sensitive, and easily performed screening method that could be multiplexed for the simultaneous detection of several marine toxins.
Asunto(s)
Bungarotoxinas/análisis , Citometría de Flujo/métodos , Iminas/análisis , Microesferas , Compuestos de Espiro/análisis , Animales , Biotina/química , Biotina/metabolismo , Bungarotoxinas/metabolismo , Proteínas Portadoras/química , Proteínas Portadoras/metabolismo , Compuestos Heterocíclicos con 3 Anillos/análisis , Compuestos Heterocíclicos con 3 Anillos/metabolismo , Hidrocarburos Cíclicos/análisis , Hidrocarburos Cíclicos/metabolismo , Iminas/metabolismo , Proteínas Inmovilizadas/química , Proteínas Inmovilizadas/metabolismo , Lymnaea/metabolismo , Ficoeritrina/química , Unión Proteica , Receptores Nicotínicos/química , Receptores Nicotínicos/metabolismo , Mariscos/análisis , Compuestos de Espiro/metabolismo , Estreptavidina/química , Estreptavidina/metabolismo , Torpedo/metabolismoAsunto(s)
Contaminantes Atmosféricos/toxicidad , Contaminación del Aire/efectos adversos , Exposición por Inhalación/efectos adversos , Material Particulado/toxicidad , Trastornos Respiratorios/epidemiología , Contaminantes Atmosféricos/análisis , Contaminación del Aire/análisis , Carbono/análisis , Carbono/toxicidad , Monitoreo del Ambiente , Humanos , Hidrocarburos Cíclicos/análisis , Hidrocarburos Cíclicos/toxicidad , Exposición por Inhalación/análisis , Tamaño de la Partícula , Material Particulado/análisis , Trastornos Respiratorios/etiología , Medición de Riesgo , Factores de RiesgoRESUMEN
The chemical composition, antimicrobial activity, total phenol content, total antioxidant activity, and total oxidant status of the essential oil from Micromeria congesta Boiss. & Hausskn. ex Boiss. were investigated. Steam distillation was used to obtain the essential oil, and the chemical analyses were performed by gas chromatography-mass spectrometry. The antimicrobial activity was tested by an agar disc diffusion method against the tested microorganisms: Bacillus subtilis NRRL B-744, Bacillus cereus NRRL B-3711, Staphylococcus aureus ATCC 12598, S. aureus ATCC 25923, S. aureus ATCC 25933, Escherichia coli 0157H7, E. coli ATCC25922, Micrococcus luteus NRLL B-4375, Enterococcus faecalis ATCC 19433, Proteus vulgaris RSKK 96026, and Yersinia enterecolitica RSKK 1501. The major compounds found in volatiles of M. congesta were piperitone oxide, linalool oxide, veratrole, pulegone, dihydro carvone, naphthalene, iso-menthone, para-menthone, and cyclohexanone. Compared to that of reference antibiotics, the antibacterial activity of the essential oil is considered as significant. Results showed that M. congesta has the potential for being used in food and medicine depending on its antioxidant and antibacterial activity.
Asunto(s)
Antiinfecciosos/farmacología , Antioxidantes/farmacología , Lamiaceae/química , Aceites Volátiles/farmacología , Componentes Aéreos de las Plantas/química , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Bacillales/efectos de los fármacos , Bacillales/crecimiento & desarrollo , Pruebas Antimicrobianas de Difusión por Disco , Etnofarmacología , Conservantes de Alimentos/química , Conservantes de Alimentos/aislamiento & purificación , Conservantes de Alimentos/farmacología , Bacterias Gramnegativas/efectos de los fármacos , Bacterias Gramnegativas/crecimiento & desarrollo , Hidrocarburos Cíclicos/análisis , Lamiaceae/crecimiento & desarrollo , Aceites Volátiles/química , Aceites Volátiles/aislamiento & purificación , Fenoles/análisis , Componentes Aéreos de las Plantas/crecimiento & desarrollo , Conservadores Farmacéuticos/química , Conservadores Farmacéuticos/aislamiento & purificación , Conservadores Farmacéuticos/farmacología , Terpenos/análisis , TurquíaRESUMEN
The naphthenic acids of oil sands process-affected water (OSPW) are said to be important toxicants. The major acids are stated to have alicyclic structures and recently, numerous of these have been identified, but some evidence suggests 'aromatic' acids are also present. The proportions of such acids have not been reported because they exist in so-called supercomplex mixtures with the alicyclic species. Their contribution to the toxicity of OSPW, if any, is therefore unknown. Here we report the use of multidimensional comprehensive gas chromatography-mass spectrometry (GC×GC-MS) with polar first dimension and non-polar second dimension GC columns and argentation solid phase extraction, to separate methyl esters of the acids of an OSPW supercomplex, into distinct fractions. A major fraction (ca 70%) was shown to contain acids (methyl esters) previously identified as alicyclic species. Authentic adamantane acid methyl esters were shown to chromatograph in this fraction. This fraction was isolated by argentation solid phase extraction (SPE) by elution with hexane. GC-MS and GC×GC-MS confirmed this to be the major fraction in the original supercomplex containing alicyclic acids (methyl esters). A second fraction shown to contain monoaromatic acids (methyl esters) by GC×GC-MS was unexpectedly abundant (ca 30% relative to the acyclic acids). The naphtheno-aromatic dehydroabietic acid was confirmed by co-injection with an authentic compound and several acids previously tentatively identified as naphtheno-monoaromatics were present. This fraction was isolated by argentation SPE by elution with more polar 5% diethyl ether in hexane. GC-MS and GC×GC-MS confirmed that the fraction represented a significant proportion of the original supercomplex. A further fraction, eluting from the argentation SPE column with more 5% diethyl ether in hexane in the same retention volume as authentic methyl naphthoate, contained, in addition to some of the second fraction, a third, much more minor complex. This had somewhat similar GC×GC retention characteristics to that of methyl naphthoate and the methyl ester of authentic fluorene-9-carboxylic acid. Spectra are reported. Non-alicyclic, mono- and possibly diaromatic acids are a much more quantitatively significant proportion of OSPW than previously realised. The individual acids need to be better identified and the toxicity of these and other SPE fractions studied in order to assess any possible environmental effects of OSPW.