RESUMEN

OBJECTIVE: Rhizoctonia solani is a soil-borne fungal pathogen of many important crop plants. In rice, R. solani causes sheath blight disease, which results in devastating grain yield and quality losses. Few methods are available to control this pathogen and classic single gene resistance mechanisms in rice plants have not been identified. We hypothesize that alternate means of control are available in the environment including free-living amoebae. Amoebae are soil-, water- and air-borne microorganisms that are predominantly heterotrophic. Many amoeba species are mycophagous, and several harm their prey using mechanisms other than phagocytosis. Here, we used light and scanning electron microscopy to survey the interactions of R. solani with four amoeba species, with the goal of identifying amoebae species with potential for biocontrol. RESULTS: We observed a wide range of responses during interactions of R. solani with four different free-living amoebae. Two Acanthamoeba species encyst in co-cultures with R. solani at higher rates than medium without R. solani. Vermamoeba vermiformis (formerly Hartmanella vermiformis) attach to R. solani mycelium and are associated with mycelial shriveling and perforations of fungal cell walls, indicating an antagonistic interaction. No phenotypic changes were observed in co-cultures of Dictyostelium discoideum and R. solani.

Asunto(s)

Acanthamoeba/fisiología , Antibiosis , Hartmannella/fisiología , Micelio/ultraestructura , Control Biológico de Vectores/métodos , Rhizoctonia/ultraestructura , Acanthamoeba/microbiología , Acanthamoeba/ultraestructura , Agentes de Control Biológico/metabolismo , Agentes de Control Biológico/farmacología , Pared Celular/química , Pared Celular/efectos de los fármacos , Pared Celular/ultraestructura , Técnicas de Cocultivo , Dictyostelium/microbiología , Dictyostelium/fisiología , Dictyostelium/ultraestructura , Hartmannella/microbiología , Hartmannella/ultraestructura , Micelio/efectos de los fármacos , Micelio/crecimiento & desarrollo , Micelio/patogenicidad , Oryza/microbiología , Enfermedades de las Plantas/prevención & control , Rhizoctonia/efectos de los fármacos , Rhizoctonia/crecimiento & desarrollo , Rhizoctonia/patogenicidad

RESUMEN

Amoebae serve as hosts for various intracellular bacteria, including human pathogens. These microbes are able to overcome amoebal defense mechanisms and successfully establish a niche for replication, which is usually the cytoplasm. Here, we report on the discovery of a bacterial symbiont that is located inside the nucleus of its Hartmannella sp. host. This symbiont, tentatively named 'Candidatus Nucleicultrix amoebiphila', is only moderately related to known bacteria (∼90% 16S and 23S rRNA sequence similarity) and member of a novel clade of protist symbionts affiliated with the Rickettsiales and Rhodospirillales. Screening of 16S rRNA amplicon data sets revealed a broad distribution of these bacteria in freshwater and soil habitats. 'Candidatus Nucleicultrix amoebiphila' traffics within 6 h post infection to the host nucleus. Maximum infection levels are reached after 96-120 h, at which time point the nucleus is pronouncedly enlarged and filled with bacteria. Transmission of the symbionts occurs vertically upon host cell division but may also occur horizontally through host cell lysis. Although we observed no impact on the fitness of the original Hartmannella sp. host, the bacteria are rather lytic for Acanthamoeba castellanii. Intranuclear symbiosis is an exceptional phenomenon, and amoebae represent an ideal model system to further investigate evolution and underlying molecular mechanisms of these unique microbial associations.

Asunto(s)

Alphaproteobacteria/clasificación , Núcleo Celular/microbiología , Hartmannella/microbiología , Acanthamoeba/microbiología , Alphaproteobacteria/genética , Alphaproteobacteria/aislamiento & purificación , Hartmannella/ultraestructura , Especificidad del Huésped , Filogenia , Simbiosis

RESUMEN

Legionella pneumophila is known as a facultative intracellular parasite of free-living soil and freshwater amoebae, of which several species have been shown to support the growth of the pathogenic bacteria. We report for the first time the behaviour of two strains (c2c and Z503) of the amoeba Willaertia magna towards different strains of L. pneumophila serogroup 1 and compared it with Acanthamoeba castellanii and Hartmannella vermiformis, known to be L. pneumophila permissive. In contrast to the results seen with other amoebae, W. magna c2c inhibited the growth of one strain of Legionella (L. pneumophila, Paris), but not of others belonging to the same serogroup (L. pneumophila, Philadelphia and L. pneumophila, Lens). Also, the different L. pneumophila inhibited cell growth and induced cell death in A. castellanii, H. vermiformis and W. magna Z503 within 3-4 days while W. magna c2c strain remained unaffected even up to 7 days. Electron microscopy demonstrated that the formation of numerous replicative phagosomes observed within Acanthamoeba and Hartmannella is rarely seen in W. magna c2c cocultured with L. pneumophila. Moreover, the morphological differences were observed between L. pneumophila cultured either with Willaertia or other amoebae. These observations show that amoebae are not all equally permissive to L. pneumophila and highlight W. magna c2c as particularly resistant towards some strains of this bacterium.

Asunto(s)

Acanthamoeba castellanii/microbiología , Eucariontes/microbiología , Hartmannella/microbiología , Interacciones Huésped-Patógeno , Legionella pneumophila/fisiología , Acanthamoeba castellanii/crecimiento & desarrollo , Acanthamoeba castellanii/ultraestructura , Animales , Técnicas de Cocultivo , Eucariontes/crecimiento & desarrollo , Eucariontes/ultraestructura , Agua Dulce , Hartmannella/crecimiento & desarrollo , Hartmannella/ultraestructura , Humanos , Legionella pneumophila/patogenicidad , Microscopía Electrónica de Transmisión , Especificidad de la Especie

RESUMEN

Based on morphological and molecular characterisation, four amoeba strains isolated from organs of freshwater fish were identified as Hartmannella vermiformis Page, 1967. Small subunit rRNA gene sequences of these strains expand the set of corresponding complete and almost complete sequences of this species to twelve. A new species-specific oligonucleotide probe inferred from recently available SSU rRNA gene sequences was designed and successfully tested in tissue lesions produced by one strain of H. vermiformis in experimentally infected fish.

Asunto(s)

Amebiasis/veterinaria , Enfermedades de los Peces/parasitología , Hartmannella/genética , Hartmannella/ultraestructura , Filogenia , Amebiasis/diagnóstico , Amebiasis/parasitología , Animales , Secuencia de Bases/genética , Enfermedades de los Peces/diagnóstico , Peces , Genes de ARNr/genética , Hartmannella/clasificación , Hartmannella/aislamiento & purificación , Hibridación Fluorescente in Situ/métodos , Funciones de Verosimilitud , Microscopía Electrónica/métodos , Datos de Secuencia Molecular , Análisis de Secuencia de ADN

RESUMEN

The morphology of Legionella pneumophila grown within Hartmanella vermiformis was studied by electron microscopy. Different morphologies were observed, including a replicative form and a mature intracellular form, that had previously been reported in Hela cells. L. pneumophila was also detected within the cyst wall of the amoeba.

Asunto(s)

Hartmannella/microbiología , Legionella pneumophila/fisiología , Animales , Hartmannella/ultraestructura , Legionella pneumophila/patogenicidad , Legionella pneumophila/ultraestructura , Microscopía Electrónica

RESUMEN

In contrast to Legionella pneumophila, little is known about the pathogenesis of other legionellae species that are capable of causing Legionnaires' disease. In this report, we contrast L. pneumophila and L. micdadei for their cytopathogenicity and intracellular replication within mammalian and protozoan cells. We show by transmission electron microscopy that L. micdadei replicates within an endoplasmic reticulum (RER)-free phagosome within human macrophages, alveolar epithelial cells, and within the protozoan Hartmannella vermiformis. In contrast, L. pneumophila replicates within a RER-surrounded phagosome within the same host cells. In contrast to replication of L. pneumophila within Acanthamoebae polyphaga, L. micdadei does not replicate within this protozoan host. Despite the prolific intracellular replication, L. micdadei is less cytopathogenic to all host cells than L. pneumophila. Since both species replicate intracellularly to a similar level, we have examined whether the reduced cytopathogenicity of L. micdadei is due to a reduced capacity to induce apoptosis or pore formation-mediated necrosis, both of which contribute to killing of the host cell by L. pneumophila. The data show that both species induced apoptosis-mediated killing of mammalian cells to a similar level. In contrast to L. pneumophila, expression of the pore-forming toxin by L. micdadei and its necrotic effect on macrophages and alveolar epithelial cells is undetectable. This has been further confirmed showing that L. micdadei is completely defective in contact-dependent haemolysis of RBCs, an activity mediated by the pore-forming toxin. Finally, in contrast to L. pneumophila, there was no significant intrapulmonary replication of L. micdadei in the A/J mice animal model. Our data show dramatic differences between L. pneumophila and L. micdadei in intracellular replication, cytopathogenicity, and infectivity to mammalian and protozoan cells.

Asunto(s)

Amébidos/microbiología , Legionella/crecimiento & desarrollo , Legionella/patogenicidad , Macrófagos/microbiología , Alveolos Pulmonares/microbiología , Acanthamoeba/microbiología , Acanthamoeba/ultraestructura , Animales , Apoptosis , Células Epiteliales/microbiología , Femenino , Hartmannella/microbiología , Hartmannella/ultraestructura , Humanos , Etiquetado Corte-Fin in Situ , Legionella pneumophila/crecimiento & desarrollo , Legionella pneumophila/patogenicidad , Pulmón/microbiología , Macrófagos/ultraestructura , Ratones , Microscopía Electrónica , Alveolos Pulmonares/citología , Alveolos Pulmonares/ultraestructura , Virulencia

RESUMEN

The intracellular pathogens Legionella micdadei and Legionella pneumophila are the two most common Legionella species that cause Legionnaires' disease. Intracellular replication within pulmonary cells is the hallmark of Legionnaires' disease. In the environment, legionellae are parasites of protozoans, and intracellular bacterial replication within protozoans plays a major role in the transmission of Legionnaires' disease. In this study, we characterized the initial host signal transduction mechanisms involved during attachment to and invasion of the protozoan host Hartmannella vermiformis by L. micdadei. Bacterial attachment prior to invasion of H. vermiformis by L. micdadei is associated with tyrosine dephosphorylation of multiple host cell proteins, including a 170-kDa protein. We have previously shown that this 170-kDa protein is the galactose N-acetylgalactosamine (Gal/GalNAc)-inhibitable lectin receptor that mediates attachment to and invasion of H. vermiformis by L. pneumophila. Subsequent bacterial entry targets L. micdadei into a phagosome that is not surrounded by the rough endoplasmic reticulum (RER). In contrast, uptake of L. pneumophila mediated by attachment to the Gal/GalNAc lectin is followed by targeting of the bacterium into an RER-surrounded phagosome. These results indicate that despite similarities in the L. micdadei and L. pneumophila attachment-mediated signal transduction mechanisms in H. vermiformis, the two bacterial species are targeted into morphologically distinct phagosomes in their natural protozoan host.

Asunto(s)

Hartmannella/metabolismo , Hartmannella/microbiología , Legionella/fisiología , Fagosomas/microbiología , Transducción de Señal , Acetilgalactosamina/farmacología , Animales , Adhesión Bacteriana , Retículo Endoplásmico/ultraestructura , Galactosa/farmacología , Hartmannella/genética , Hartmannella/ultraestructura , Lectinas/metabolismo , Legionella/ultraestructura , Microscopía Electrónica , Fagosomas/ultraestructura , Fosforilación , Proteínas Protozoarias/metabolismo , Tirosina/metabolismo

RESUMEN

The Legionnaires' disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen that invades and replicates within two evolutionarily distant hosts, free living protozoa and mammalian cells. Invasion and intracellular replication within protozoa are thought to be major factors in the transmission of Legionnaires' disease. We have recently reported the identification of a galactose/N-acetyl-D-galactosamine (Gal/GalNAc) lectin in the protozoan host Hartmannella vermiformis as a receptor for attachment and invasion by L. pneumophila (Venkataraman, C., B.J. Haack, S. Bondada, and Y.A. Kwaik. 1997. J. Exp. Med. 186:537-547). In this report, we extended our studies to the effects of bacterial attachment and invasion on the cytoskeletal proteins of H. vermiformis. We first identified the presence of many protozoan cytoskeletal proteins that were putative homologues to their mammalian counterparts, including actin, pp125(FAK), paxillin, and vinculin, all of which were basally tyrosine phosphorylated in resting H. vermiformis. In addition to L. pneumophila-induced tyrosine dephosphorylation of the lectin, bacterial attachment and invasion was associated with tyrosine dephosphorylation of paxillin, pp125(FAK), and vinculin, whereas actin was minimally affected. Inhibition of bacterial attachment to H. vermiformis by Gal or GalNAc monomers blocked bacteria-induced tyrosine dephosphorylation of detergent-insoluble proteins. In contrast, inhibition of bacterial invasion but not attachment failed to block bacteria-induced tyrosine dephosphorylation of H. vermiformis proteins. This was further supported by the observation that 10 mutants of L. pneumophila that were defective in invasion of H. vermiformis were capable of inducing tyrosine dephosphorylation of H. vermiformis proteins. Entry of L. pneumophila into H. vermiformis was predominantly mediated by noncoated receptor-mediated endocytosis (93%) but coiling phagocytosis was infrequently observed (7%). We conclude that attachment but not invasion by L. pneumophila into H. vermiformis was sufficient and essential to induce protein tyrosine dephosphorylation in H. vermiformis. These manipulations of host cell processes were associated with, or followed by, entry of the bacteria by a noncoated receptor-mediated endocytosis. A model for attachment and entry of L. pneumophila into H. vermiformis is proposed.

Asunto(s)

Actinas/metabolismo , Moléculas de Adhesión Celular/metabolismo , Proteínas del Citoesqueleto/metabolismo , Hartmannella/metabolismo , Legionella pneumophila/metabolismo , Enfermedad de los Legionarios/microbiología , Fosfoproteínas/metabolismo , Proteínas Tirosina Fosfatasas/metabolismo , Proteínas Tirosina Quinasas/metabolismo , Vinculina/metabolismo , Animales , Detergentes , Quinasa 1 de Adhesión Focal , Proteína-Tirosina Quinasas de Adhesión Focal , Hartmannella/ultraestructura , Humanos , Legionella pneumophila/ultraestructura , Octoxinol , Paxillin , Fosforilación , Especificidad por Sustrato , Tirosina/metabolismo

RESUMEN

Coiling phagocytosis has been observed previously only by chance, and there has been no systematic investigation of this uptake mechanism. Therefore, a comparative electron microscopical study was performed. Different human and murine cell populations, phagocytes from various vertebrate and invertebrate species, and predatory amoebae were incubated with Borrelia burgdorferi, one of the microbes known to induce coiling phagocytosis, to study the uptake mechanisms used. In this model, coiling phagocytosis was observed with both vertebrate and invertebrate species but not with amoebae. With cells from humans and mice, this uptake mechanism was restricted to phagocytic cells of myeloid origin. The coiled membrane gaps did not give rise to phagosomes; instead, membrane fusion was followed by membrane dissipation. Thus, coiling of B. burgdorferi apparently is an alternative uptake mechanism used by metazoan phagocytes, involving special membrane processing. However, coiling phagocytosis may show different features with different microbes.

Asunto(s)

Fagocitos/fisiología , Fagocitosis/fisiología , Animales , Grupo Borrelia Burgdorferi/ultraestructura , Línea Celular , Entamoeba histolytica/ultraestructura , Hartmannella/ultraestructura , Humanos , Macrófagos Peritoneales/fisiología , Macrófagos Peritoneales/ultraestructura , Ratones , Fagocitos/ultraestructura , Fagosomas/fisiología , Fagosomas/ultraestructura , Filogenia

RESUMEN

Legionella pneumophila is an intracellular parasite of protozoa and human phagocytes. To examine adaptation of this bacterium to parasitize protozoa, the sequence of events of the intracellular infection of the amoeba Hartmannella vermiformis was examined. The previously described uptake phenomenon of coiling phagocytosis by human monocytes was not detected. A 1 h postinfection with wild-type strain AA100, mitochondria were observed within the vicinity of the phagosome. At 2.5 h postinfection, numerous vesicles surrounded the phagosomes and mitochondria were in close proximity to the phagosome. At 5 h postinfection, the bacterium was surrounded by a ribosome-studded multilayer membrane. Bacterial multiplication was evident by 8 h postinfection, and the phagosome was surrounded by a ribosome-studded multilayer membrane until 15 h postinfection. The recruitment of organelles and formation of the ribosome-studded phagosome was defective in an isogenic attenuated mutant of L. pneumophila (strain AA101A) that failed to replicate within amoebae. At 20 h postinfection with wild-type strain AA100, numerous bacteria were present in the phagosome and ribosome were not detected around the phagosome. These data showed that, at the ultrastructural level, the intracellular infection of protozoa by L. pneumophila is highly similar to that of infection of macrophages. Immunocytochemical studies provided evidence that at 5 h postinfection the phagosome containing L. pneumophila acquired an abundant amount of the endoplasmic reticulum-specific protein (BiP). Similar to phagosomes containing heat-killed wild-type L. pneumophila, the BiP protein was not detectable in phagosomes containing the mutant strain AA101A. In addition to the absence of ribosomes and mitochondria, the BiP protein was not detected in the phagosomes at 20 h postinfection with wild-type L. pneumophila. The data indicated that the ability of L. pneumophila to establish the intracellular infection of amoebae is dependent on its capacity to reside and multiply within a phagosome surrounded by the rough endoplasmic reticulum. This compartment may constitute a rich source of nutrients for the bacteria and is probably recognized as cellular compartment. The remarkable similarity of the intracellular infections of macrophages and protozoa by L. pneumophila strongly supports the hypothesis that adaptation of the bacterium to the intracellular environment of protozoa may be the mechanism for its ability to adapt to the intracellular environment of human alveolar macrophages and causes pneumonia.

Asunto(s)

Hartmannella/microbiología , Legionella pneumophila/patogenicidad , Fagosomas/microbiología , Adaptación Fisiológica , Animales , Retículo Endoplásmico Rugoso/ultraestructura , Hartmannella/ultraestructura , Humanos , Legionella pneumophila/fisiología , Legionella pneumophila/ultraestructura , Microscopía Electrónica , Fagosomas/ultraestructura

RESUMEN

PURPOSE: To support the hypothesis that Acanthamoeba is not a unique cause of amebic keratitis, we report a case of amebic keratitis in which viable Acanthamoeba could not be isolated from corneal tissue. Vahlkampfia and Hartmannella, two other genera of free-living ameba, were isolated, however, using prolonged culture. METHODS: A 24-year-old wearer of soft contact lenses had keratitis. Extensive histologic and microbiologic investigations were performed on corneal scrape, biopsy, and keratoplasty tissue. Contact lenses, storage case, and the home water supply, where contact lens hygiene was practiced, were examined for the presence of micro-organisms. RESULTS: No viruses, pathogenic bacteria, or fungi were detected from corneal tissue samples. Amebae were observed using light and electron microscopy, but these could not be unequivocally classified using immunocytochemical staining. Viable Vahlkampfia and Hartmannella, but no Acanthamoeba, were isolated from the corneal biopsy sample. Indirect immunofluorescence with a range of polyclonal rabbit antisera raised against axenically cultivated stains of the three amebal genera was unhelpful because of cross-reactivity. A diverse range of micro-organisms was present within the storage case, including the three amebal species. Amebic cysts also were associated with the contact lens. CONCLUSION: A mixed non-Acanthamoeba amebic keratitis has been identified in a wearer of soft contact lenses where lack of storage case hygiene provided the opportunity for the free-living protozoa Vahlkampfia and Hartmannella to be introduced to the ocular surface. When Acanthamoeba-like keratitis occurs, but where Acanthamoeba cannot be isolated using conventional laboratory culture methods, alternate means should be used to identify other amebae that may be present. Polyclonal immunofluorescent antibody staining was unreliable for generic identification of pathogenic free-living amebae in corneal tissue.

Asunto(s)

Amebiasis/etiología , Amébidos/aislamiento & purificación , Lentes de Contacto Hidrofílicos/efectos adversos , Infecciones Parasitarias del Ojo/etiología , Hartmannella/aislamiento & purificación , Queratitis/etiología , Adulto , Amebiasis/patología , Amebiasis/terapia , Amébidos/inmunología , Amébidos/ultraestructura , Animales , Anticuerpos Antiprotozoarios/inmunología , Antifúngicos/uso terapéutico , Antígenos de Protozoos/análisis , Córnea/parasitología , Córnea/ultraestructura , Equipos Desechables , Infecciones Parasitarias del Ojo/patología , Infecciones Parasitarias del Ojo/terapia , Técnica del Anticuerpo Fluorescente Indirecta , Hartmannella/inmunología , Hartmannella/ultraestructura , Humanos , Queratitis/patología , Queratitis/terapia , Queratoplastia Penetrante , Masculino , Conejos

RESUMEN

Meningoencephalitis and bronchopneumonia were documented in a patient from Peubla, Mexico. The patient began with symptoms and signs of a common flu and 12 days after the onset of his disease he was admitted to the hospital presenting symptoms and signs of meningoencephalitis. The clinical course evolved into an endocraneal hypertension syndrome with bronchopneumonia, coma and death. Wide-spectrum antibiotics, immunosuppressive and anti-tuberculosis therapy were unsuccessfully administered. Important antecedents were degree I malnutrition and repeated contact with polluted water. Post-mortem autopsy was not performed. Gram-positive cocci were isolated from the spinal fluid 2 days after admission, and then active amebae were isolated from three different samples of the spinal fluid at days 16, 18 and 19 after admission. Such samples were concentrated and inoculated onto specific culture media. Identification of amebae was based on their morphology and biochemistry. All amebae were Hartmannella vermiformis. Amebae were apparently not the cause of the disease and might be considered as an opportunistic colonizer which may have caused the evolution of the disease to become worse.

Asunto(s)

Amebiasis/complicaciones , Bronconeumonía/patología , Líquido Cefalorraquídeo/parasitología , Hartmannella/aislamiento & purificación , Meningoencefalitis/patología , Fosfatasa Ácida/química , Adolescente , Amebiasis/patología , Animales , Bronconeumonía/terapia , Hidrolasas de Éster Carboxílico/química , Líquido Cefalorraquídeo/microbiología , Electroforesis en Gel de Agar/métodos , Resultado Fatal , Glucosa-6-Fosfato Isomerasa/química , Hartmannella/clasificación , Hartmannella/ultraestructura , Humanos , Focalización Isoeléctrica/métodos , Leucil Aminopeptidasa/química , Masculino , Meningoencefalitis/terapia , Infecciones Oportunistas , Fosfoglucomutasa/química

RESUMEN

Legionella pneumophila is an intracellular parasite of Hartmannella vermiformis. Attachment to the amebae and entry of L. pneumophila were studied by two quantitative assays: One used plate counts to measure the number of bacteria attaching to amebae at 4 degrees C; the other determined the number of intracellular bacteria by use of transmission electron microscopy (TEM). The attachment assay showed that L. pneumophila are inefficient in attachment to amebae. About 0.05% of the bacteria were bound after 1 h with a 10- to 40-fold increase over the next 11 h. Attachment of both virulent and avirulent strains of L. pneumophila occurred at a similar rate. Uptake of L. pneumophila was measured by counting intracellular bacteria using TEM. Limited numbers of virulent L. pneumophila were found intracellularly before 4 h, but the numbers increased logarithmically after this time. The number of amebae containing virulent L. pneumophila increased linearly during the 12-h co-incubation. Avirulent L. pneumophila were rarely detected within amebae throughout the 12-h incubation. Results indicate that entry, not attachment, of virulent L. pneumophila is the limiting step in infection of axenically grown H. vermiformis.

Asunto(s)

Hartmannella/microbiología , Legionella pneumophila/fisiología , Animales , Adhesión Bacteriana , Hartmannella/ultraestructura , Legionella pneumophila/patogenicidad , Legionella pneumophila/ultraestructura , Microscopía Electrónica de Rastreo , Virulencia

RESUMEN

Some aspects of relationships between soil ameba and the causative agents of plague and pseudotuberculosis, capable of forming natural associations, are considered. Ameba can phagocytose bacteria causing plague and pseudotuberculosis. Cases of the preservation of individual bacterial cells at the stationary phase and in precysts of amebae have been registered.

Asunto(s)

Hartmannella/fisiología , Microbiología del Suelo , Yersinia pestis/fisiología , Yersinia pseudotuberculosis/fisiología , Animales , Quimiotaxis , Ecología , Hartmannella/microbiología , Hartmannella/ultraestructura , Microscopía Electrónica , Fagocitosis , Factores de Tiempo , Yersinia pestis/ultraestructura , Yersinia pseudotuberculosis/ultraestructura

RESUMEN

Between October 1987 and March 1989, we tested 144 water samples obtained from the plumbing and cooling tower systems of 5 Paris hospitals for the presence of legionellae and amoebae. Of the samples tested for Legionella, 67 out of 144 (46.5%) were positive, and 82 out of 116 tested for amoebae (70.7%) were positive. The ability of protozoa to support the multiplication of legionella was shown by incubating samples at 35.5 degrees C for 7-15 days. Prior to determining the presence of legionellae and amoebae, 51 of the 144 samples were incubated. After incubation, 22 out of 25 (88%) samples which were positive for the presence of both Legionella and amoebae showed multiplication of Legionella. In 3 out of the 25 (12%) samples containing Legionella and amoebae, Legionella failed to multiply. Six out of the 51 (11.8%) samples which were negative in direct culture for Legionella but positive for amoebae, became positive after incubation. Legionella did not multiply in samples negative for amoebae, nor was there proliferation in samples after filtration through a 1.2-microns membrane followed by incubation for the same period and temperature. Strains of Legionella pneumophila serogroup 1 and serogroup 6 (SG1 and SG6), including 3 patient isolates and 2 environmental isolates, were cocultured with 2 strains of amoebae and Tetrahymena pyriformis. Plate counts, Gimenez staining and electron microscopy demonstrated that intracellular legionellae proliferation occurred.

Asunto(s)

Amoeba/aislamiento & purificación , Infección Hospitalaria/microbiología , Legionella/crecimiento & desarrollo , Microbiología del Agua , Aire Acondicionado , Amoeba/microbiología , Animales , Francia , Hartmannella/microbiología , Hartmannella/ultraestructura , Unidades Hospitalarias , Humanos , Técnicas In Vitro , Legionella/aislamiento & purificación , Microscopía Electrónica , Ingeniería Sanitaria , Tetrahymena pyriformis/microbiología , Tetrahymena pyriformis/ultraestructura

RESUMEN

A cloned and axenically cultured strain of Hartmannella vermiformis was used as a model to study intracellular multiplication of Legionella pneumophila in amoebae. The growth of L. pneumophilia in both H. vermiformis and a human monocyte-like cell line (U937) was investigated with cytoskeletal and metabolic inhibitors. L. pneumophila replicated only intracellularly in these cellular models, and electron microscopy showed ultrastructural similarities in the initial phase of multiplication. Treatment of amoebae with an inhibitor of microfilament-dependent phagocytosis (cytochalasin D, 0.5 or 1.0 micrograms/ml) did not inhibit intracellular growth of L. pneumophila; however, intracellular multiplication was inhibited by treatment of U937 monocytes with the same concentrations of cytochalasin D. Methylamine (10 to 100 mM), an inhibitor of adsorptive pinocytosis, inhibited the replication of L. pneumophila in amoebae in a dose-dependent manner. All doses of methylamine tested (10 to 50 mM) inhibited growth of L. pneumophila in U937 monocytes. Cytochalasin D and methylamine had no effect on the multiplication of L. pneumophila in culture medium or on the viability of amoebae or U937 monocytes. Intracellular replication of L. pneumophila in H. vermiformis may be accomplished by a cytochalasin D-independent mechanism, such as adsorptive pinocytosis. In contrast, both cytochalasin D- and methylamine-sensitive mechanisms may be essential for the intracellular multiplication of L. pneumophila in U937 monocytes.

Asunto(s)

Citocalasina D/farmacología , Hartmannella/microbiología , Legionella/efectos de los fármacos , Metilaminas/farmacología , Monocitos/microbiología , Análisis de Varianza , Animales , Línea Celular , Recuento de Colonia Microbiana , Hartmannella/efectos de los fármacos , Hartmannella/ultraestructura , Humanos , Legionella/crecimiento & desarrollo , Legionella/ultraestructura , Monocitos/efectos de los fármacos , Monocitos/ultraestructura

RESUMEN

In this study cyst walls of Hartmannella glebae were isolated and quantitatively analyzed. They were fractionated into alkali-insoluble and alkali-soluble fractions. The alkali-insoluble fraction appeared to be cellulose which represented 4.2% of the total weight of the wall. The major component of the alkali-soluble fraction consisted of proteins (64.7%). It also contained a glucose polymer (probably a precursor of cellulose) and lipids. The amino acid composition of the wall was also determined.

Asunto(s)

Hartmannella/ultraestructura , Aminoácidos/análisis , Animales , Carbohidratos/análisis , Membrana Celular/ultraestructura , Lípidos de la Membrana/análisis , Proteínas de la Membrana/análisis , Microscopía de Contraste de Fase , Fosfatos/análisis , Especificidad de la Especie

Asunto(s)

Colesterol/metabolismo , Hartmannella/metabolismo , Metabolismo de los Lípidos , Fosfolipasas/metabolismo , Animales , Hartmannella/ultraestructura

RESUMEN

The present study was performed with the help of scanning electron microscope and refers to the external morphology of the cysts of the free-living amoebae. The amoebae belonging to the genus Naegleria form cysts whose outer layer shows no wrinkles. It can be smooth (N. fowleri) or rough (N. gruberi). The average number of pores varies between 1.2 and 7.2. Their margin can be smooth (N. fowleri) or pierced (N. gruberi). The thickness of the bordering cell wall varies between 0.4 and 1.0 micrometer and that of the pore is about 0.6 micrometer. The cysts of the genera Schizopyrenus, Tetramitus and Didascalus are very small, show no wrinkles in their cell wall and contain no pores. In contrast, cysts in the general Acanthamoeba show their outer layer superficially plugged with a lesser number of wrinkles (A. rhysodes, A. culbertsoni) or with large or deep wrinkles inside their wall (. castellanii, A. polyphaga). There are no pores observed in their cysts. Thus the distinguishing morphological features of the cysts have generated important role in the taxonomy of the amoebae of the limax-group.

Asunto(s)

Amoeba/ultraestructura , Hartmannella/ultraestructura , Amoeba/clasificación , Animales , Microscopía Electrónica de Rastreo

RESUMEN

It was made possible to study the mode of nuclear division during all the stage of mitotic division of the free-living amoebae belonging to the families Schizopyrenidae and Hartmannellidae, when the massive number of them were fixed and stained. While the four phases of nuclear division vary in different genera, in the amoebae belonging to the same genus the mitotic division was similar. In the family Schizopyrenidae, the nucleus contains a central Feulgen-negative nucleolus which during mitosis form "polar masses". In the family Hartmannellidae, the resting nucleus has either a single Feulgen-negative nucleolus or several nucleoli. During mitosis the nucleolus or nucleoli disappear and a spindle with chromosomes arranged as an equatorial plate develops. The mitotic apparatus is an essential criterion in the differentiation of the genera Hartmannella and Naegleria.

Asunto(s)

Amoeba/ultraestructura , Animales , División Celular , Hartmannella/ultraestructura , Mitosis