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This study aimed to identify different environmental microbiota in animal farms adjacent to produce fields and to understand their potential flow pattern. Soil and water samples were collected from 16 locations during the winter, spring, summer, and fall seasons. In addition, a high-resolution digital elevation model helped to create a stream network to understand the potential flow of the microbiome. Metagenomic analysis of the 16 S rRNA gene revealed that soil and water samples from the four seasons harbor diverse microbiome profiles. The phylogenetic relationship of operational taxonomic units (OTUs) is separated by a maximum of 0.6 Bray-Curtis distance. Similarly, the Principal Component Analysis (P = 0.001) demonstrated the soil and water microbiome clustering across different locations and seasons. The relative abundance of Proteobacteria, Bacteroidetes, and Firmicutes was higher in the water samples than in the soil samples. In contrast, the relative abundance of Actinobacteria and Chloroflexi was higher in the soil compared to the water samples. Soil samples in summer and water samples in spring had the highest abundance of Bacteroidetes and Firmicutes, respectively. A unique microbial community structure was found in water samples, with an increased abundance of Hydrogenophaga and Solirubrobacter. Genera that were significantly abundant at a 1% false discovery rate (FDR) among seasons and soil or water samples, include Nocardioides, Gemmatimonas, JG30-KF-CM45, Massilia, Gaiellales, Sphingomonas, KD4-96, Bacillus, Streptomyces, Gaiella, and Gemmatimonadaceae. The relative abundance of pathogenic genera, including Mycobacterium, Bacteroides, Nocardia, Clostridium, and Corynebacterium, were significantly (at 1% FDR) affected by seasons and environmental type. The elevation-based stream network model suggests the potential flow of microbiomes from the animal farm to the produce fields.
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Bacterias , Granjas , Microbiota , Estaciones del Año , Microbiología del Suelo , Animales , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , California , ARN Ribosómico 16S/genética , Filogenia , Microbiología del Agua , Análisis Espacio-Temporal , MetagenómicaRESUMEN
Salmonella is a major bacterial concern for public health globally. Although there are limited documentation on the prevalence of Salmonella species in Cambodia's food chain, some reports indicate that salmonellosis is a severe gastrointestinal infection in its population and especially in children. To investigate the presence of Salmonella spp., 285 food samples (75 meat, 50 seafood, and 160 leafy green vegetable samples) were randomly collected from various local markets in Phnom Penh capital and nearby farms in Cambodia. Concurrently, field observations were conducted to collect data on food hygiene and practices among the relevant actors. All food samples were analyzed using bacterial culture and plate counts, and the findings were confirmed serially with biochemical, serological, and PCR tests. The observational data on food hygiene and practices from farm to market revealed that the spread of Salmonella in the food-value chain from farm to market could pose health risks to consumers. The overall prevalence of Salmonella spp. was 48.4% (138/285), while the prevalence in meat, seafood, and vegetables was 71% (53/75), 64% (32/50), and 33% (53/160), respectively. Mean Salmonella plate count ranged from 1.2 to 7.40 log10 CFU/g, and there was no significant difference in bacterial counts between meat, seafood, and vegetable samples (p > 0.05). The most common serogroups among the isolated Salmonella spp. were B and C. These results suggest that a large proportion of meat, seafood, and vegetable products sold at local markets in Phnom Penh are contaminated with Salmonella spp. This is likely linked to inadequate hygiene and sanitation practices, including handling, storage, and preservation conditions. Observations on farms suggested that the prevalence of Salmonella in vegetables sold at the market could be linked to contamination relating to agricultural practices. Thus, controlling the spread of foodborne salmonellosis through the food-value chain from farms and retailers to consumers is warranted to enhance food safety in Cambodia.
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Granjas , Contaminación de Alimentos , Carne , Salmonella , Alimentos Marinos , Verduras , Cambodia/epidemiología , Verduras/microbiología , Salmonella/aislamiento & purificación , Salmonella/clasificación , Contaminación de Alimentos/análisis , Contaminación de Alimentos/estadística & datos numéricos , Prevalencia , Alimentos Marinos/microbiología , Carne/microbiología , Animales , Microbiología de Alimentos , Humanos , HigieneRESUMEN
BACKGROUND: Tractors and quad bikes pose a significant risk of fatal injuries among farmers, particularly affecting older farmers. This study aimed to explore the barriers and facilitators to the adoption of machine related safety behaviors among older farmers in Irish farm settings. METHOD: Four focus groups were conducted via Zoom in February 2021. Nineteen Irish farmers from four farm types participated. The discussions were audio-recorded, transcribed verbatim, and analyzed using an inductive, reflexive thematic analysis approach. The themes identified were then mapped to the COM-B (Capability-Opportunity-Motivation) model, providing a systematic theoretical basis for designing a future intervention to reduce machine-related accidents. RESULTS: The analysis identified five inductive themes that encompassed both barriers and facilitators in farm safety practices: (1) Capability to manage competing responsibilities; (2) Characteristics of the farm and its work environment; (3) Availability and affordability of resources; (4) Prevailing sociocultural opportunities; and (5) Perceived likelihood and cost-benefit analysis in safety decision-making. These themes captured the complex interplay of capability, opportunity, and motivation in farmers' decision-making processes. The study also revealed limitations in existing interventions, such as voluntary guidelines and educational methods, in effectively addressing these barriers. CONCLUSIONS: Farmers' abilities (capability), prevailing sociocultural factors, resource availability (opportunity), and their perceived consequences and benefits (motivation) affect how safely they work with machines. The study emphasizes the need for comprehensive, theory-driven approaches that consider the interplay of capability, opportunity, and motivational factors that may support or impede machine safety. Understanding the challenges faced by Irish farmers highlights potential strategies for safety intervention, and these strategies should be co-designed with farmers and attentive to the local context. PRACTICAL APPLICATIONS: The study provides a template for understanding farmers' perspectives using the COM-B model. The findings can inform the development of theoretically informed intervention strategies based on the Behavior Change Wheel framework.
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Agricultores , Grupos Focales , Humanos , Agricultores/psicología , Agricultores/estadística & datos numéricos , Masculino , Femenino , Anciano , Persona de Mediana Edad , Irlanda , Accidentes de Trabajo/prevención & control , Granjas , Agricultura , Salud Laboral , Investigación Cualitativa , Motivación , Toma de DecisionesRESUMEN
Domestic pigs serve as significant hosts and reservoirs for multiple parasite species, some specific to pigs and many others of zoonotic importance. This study aimed to assess the prevalence of intestinal parasites in pigs within a rural area in northeastern Argentina. We also examined demographic information, breeding conditions, and exposure factors associated with parasite presence. Pig feces were subjected to coprological examination through flotation and sedimentation techniques. Modified Ziehl-Neelsen technique was employed to examine oocysts of Cryptosporidium spp. In total, 29 family farms with pig pens were analyzed, and 42 stool samples were collected from pigs on these farms. At the farm level, the presence of at least one parasite species was recorded in 27 rural houses (93.1%). We found that 90.4% of pigs were parasitized, with a specific parasitoses of 10 species, with a maximum of six species in a single host. The most prevalent protozoa were Entamoeba spp. (57.1%) and Blastocystis sp. (45.2%), followed by Iodamoeba butschlii (33.3%), Neobalantidium coli (21.4%), coccidia (14.2%), Cryptosporidium spp. (9.5%) and Giardia spp. (2.3%). The most prevalent helminths were Strongylidae eggs (52.3%), Ascaris spp. (14.2%) and Trichuris spp. (2.3%). We advocate for an urgent need to implement a comprehensive prophylaxis program prioritizing general hygiene practices such as regular cleaning, removal of fecal material and renewal of drinking water. Additionally, vaccination and deworming protocols should be implemented. Furthermore, this study highlights the necessity for molecular-level evaluations to detect potential zoonotic genotypes of the identified protozoa.
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Granjas , Heces , Parasitosis Intestinales , Enfermedades de los Porcinos , Animales , Argentina/epidemiología , Enfermedades de los Porcinos/epidemiología , Enfermedades de los Porcinos/parasitología , Prevalencia , Porcinos , Parasitosis Intestinales/epidemiología , Parasitosis Intestinales/veterinaria , Parasitosis Intestinales/parasitología , Heces/parasitología , Femenino , Sus scrofa/parasitología , MasculinoRESUMEN
Tigecycline is a last-resort drug used to treat serious infections caused by multidrug-resistant bacteria. tet(X4) is a recently discovered plasmid-mediated tigecycline resistance gene that confers high-level resistance to tigecycline and other tetracyclines. Since the first discovery of tet(X4) in 2019, it has spread rapidly worldwide, and as a consequence, tigecycline has become increasingly ineffective in the clinical treatment of multidrug-resistant infections. In this study, we identified and analyzed tet(X4)-positive Escherichia coli isolates from duck farms in Hunan Province, China. In total, 976 samples were collected from nine duck farms. Antimicrobial susceptibility testing and whole-genome sequencing (WGS) were performed to establish the phenotypes and genotypes of tet(X4)-positive isolates. In addition, the genomic characteristics and transferability of tet(X4) were determined based on bioinformatics analysis and conjugation. We accordingly detected an E. coli strain harboring tet(X4) and seven other resistance genes in duck feces. Multi-locus sequence typing analysis revealed that this isolate belonged to a new clone, and subsequent genetic analysis indicated that tet(X4) was carried in a 4608-bp circular intermediate, flanked by ISVsa3-ORF2-abh elements. Moreover, it exhibited transferability to E. coli C600 with a frequency of 10-5. The detection of tet(X4)-harboring E, coli strains on duck farms enhances our understanding of tigecycline resistance dynamics. The transferable nature of the circular intermediate of tet(X4) contributing to the spread of tigecycline resistance genes poses a substantial threat to healthcare. Consequently, vigilant monitoring and proactive measures are necessary to prevent their spread.
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Antibacterianos , Patos , Infecciones por Escherichia coli , Escherichia coli , Granjas , Pruebas de Sensibilidad Microbiana , Plásmidos , Tigeciclina , Secuenciación Completa del Genoma , Patos/microbiología , Tigeciclina/farmacología , Animales , Escherichia coli/genética , Escherichia coli/efectos de los fármacos , Escherichia coli/aislamiento & purificación , Antibacterianos/farmacología , China , Plásmidos/genética , Infecciones por Escherichia coli/veterinaria , Infecciones por Escherichia coli/microbiología , Heces/microbiología , Tipificación de Secuencias Multilocus , Farmacorresistencia Bacteriana Múltiple/genética , Genotipo , Enfermedades de las Aves de Corral/microbiología , Proteínas de Escherichia coli/genética , Transferencia de Gen Horizontal , Farmacorresistencia Bacteriana/genéticaRESUMEN
BACKGROUND: African swine fever (ASF) poses a threat to the global pig industry, leading to significant economic losses and widespread disruptions in pig farming and associated sectors. In September 2023, the first case of ASF in Swedish wild boar triggered immediate responses from authorities, including the establishment of restricted zones and culling measures. A new ASF certification programme for pig herds was initiated to improve biosecurity and proactive disease management. This survey aimed to assess the sentiments and actions of Swedish pig farmers six months post-outbreak, particularly regarding biosecurity measures. Such information is important to improve preparedness for future disease threats. A questionnaire was distributed to members of the Swedish pig producers' organisation. RESULTS: A total of 113 farmers responded (response rate 27%), with the majority considering the risk of ASF reappearing in Sweden as high. The estimated cost for connecting the farms to the ASF certification programme varied greatly, with a majority identifying cost as a substantial hurdle. While many farmers sought biosecurity advice from veterinarians, 43% had not implemented suggested measures. Over one third had not received concrete measures that would fit their farms, and 14% had not received any biosecurity advice from veterinarians at all. Discussions among farmers emphasized concerns about ASF outbreaks, transmission mechanisms, and regulatory compliance, highlighting the importance of ongoing communication and knowledge exchange to address the challenges posed by ASF effectively. Additionally, participants also mentioned the role of dense wild boar populations and shortcomings in municipal food waste management as important risk factors. CONCLUSIONS: The responding farmers expressed widespread concern about new ASF outbreaks. A majority identified cost as a substantial hurdle for joining the ASF certification programme. While many farmers consulted veterinarians for advice on biosecurity, a significant number had yet to implement suggested measures and one third had not received specific guidance suitable for their farms. Stakeholder conversations highlighted concerns about ASF outbreaks, transmission, and compliance. They also discussed the role of dense wild boar populations and issues with municipal food waste management as significant risk factors for ASF.
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Fiebre Porcina Africana , Crianza de Animales Domésticos , Animales , Suecia , Porcinos , Fiebre Porcina Africana/prevención & control , Fiebre Porcina Africana/epidemiología , Crianza de Animales Domésticos/métodos , Control de Infecciones/métodos , Encuestas y Cuestionarios , Bioaseguramiento , Granjas , Agricultores/psicología , Sus scrofa , Brotes de Enfermedades/veterinaria , Brotes de Enfermedades/prevención & controlRESUMEN
OBJECTIVES: To evaluate the nutritional quality and microbial contamination of raw camel milk sourced from street vendors and compare it with milk obtained from farms, with a particular emphasis on pathogenic organisms. METHODS: Twenty samples were systematically collected from street vendors and farms between July 2022 and February 2023 and analyzed at King Fahad Medical Research Centre and the Pharmacy College, King Abdulaziz University, Jeddah, Saudi Arabia. The microbial analysis employed culture-dependent techniques for colony-forming unit analysis and isolation of microbial colonies from milk samples. Microbial identification utilized advanced methods, including VITEK-MS equipment and the MALDI-TOF technique. The chemical composition was analysed through enzyme-linked immunosorbent assays. RESULTS: The findings revealed significant differences in microbial loads, with milk sourced from street vendors exhibiting considerably higher microbial counts than farm-sourced milk, including pathogenic species like Staphylococcus aureus and Escherichia coli. The results indicated that camel milk from street vendors possessed a higher level of microbial contamination, suggesting potential health risks associated with its purchase and consumption from these sources. CONCLUSION: This study highlights the urgent need for stringent food safety practices in handling, selling, and distributing camel milk to reduce microbial risks to safe levels, thereby mitigating potential health hazards.
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Camelus , Leche , Valor Nutritivo , Animales , Arabia Saudita , Camelus/microbiología , Leche/microbiología , Humanos , Microbiología de Alimentos , Granjas , Salud Pública , Staphylococcus aureus/aislamiento & purificación , Escherichia coli/aislamiento & purificación , Comercio , Inocuidad de los Alimentos , Contaminación de Alimentos/análisisRESUMEN
The socioeconomic factors influencing small-scale dairy producers in the border area between Ecuador and Colombia were meticulously identified. Employing a non-experimental design, the study leveraged multivariate statistical analysis to discern key determinants. Data processing was executed using the statistical software SPSS v27, facilitating comprehensive analysis. A random survey was administered to 532 small and medium-scale dairy producers in the Carchi province of Ecuador, employing a structured questionnaire supplemented with a Likert scale for nuanced insights. Based on 35 original variables, seven determining factors were identified in dairy farms: political representation, adequate housing, equipment, innovation, empathy, profitability, social welfare, which combined explain 60.95% of the system's variability. Such factors affect production, the level of household income, as well as their effect on the standard of living of households. Three groups were formed, the first with a low perception of economic development (Traditionalists 33.3%); the second with a better expectation of economic development (Modernizers 27.6%); and the third, identified with greater economic development (Innovators 10.3%). Each group presents cases with a low to high standard of living perspective. The groups have peculiarities in terms of their performance that can be applied to the entire population. A significant relation was established between socioeconomic factors and standard of living.
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Industria Lechera , Factores Socioeconómicos , Ecuador , Colombia , Industria Lechera/economía , Industria Lechera/estadística & datos numéricos , Animales , Encuestas y Cuestionarios , Bovinos , Femenino , Granjas/estadística & datos numéricosRESUMEN
The study aimed to ascertain a relationship between agricultural status, socioeconomic factors, and nutrition of farm families. The study was conducted in selected villages in the West Garo Hills district of Meghalaya, using Stratified Random Sampling (St. RS). Using pretested interview schedules, we collected primary data from respondents in 2020 and 2021, focusing on socioeconomic variables, body mass index, and income from agriculture and related sectors. The data was analysed using correlation analyses and separate combined regression estimates for each year and month were obtained. Results from the study indicate that agricultural income significantly influenced nutritional status (p < 0.05) and household income growth was also found significant. The region's agricultural production of cereals, pulses, and vegetables was insufficient, as was the production of meat and meat products, milk, and milk products. Hence, expenditure towards purchasing the above food groups from the market was found to be significant (p < 0.05). Therefore, the markets near the mainland especially in the hilly region play a crucial role in the nutritional pathway of rural farm families.
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Seguridad Alimentaria , Estado Nutricional , Población Rural , Factores Socioeconómicos , Humanos , India , Femenino , Masculino , Agricultura , Adulto , Renta , Abastecimiento de Alimentos , Índice de Masa Corporal , GranjasRESUMEN
Infectious diseases in livestock not only cause significant economic losses but also affect food security. Although wildlife may be involved in these infectious diseases by serving as reservoirs, research has primarily focused on livestock and related species. Moreover, while these species represent a potential threat in wildlife-borne infectious diseases, comprehensive surveys of the presence of various species are limited. In this study, we aimed to quantitatively investigate the occurrence of various mammals and birds at three pig farms in Japan. We conducted camera trap surveys from October 15, 2020, to March 24, 2022, and determined the relative abundance index inside and outside the sanitary control zone. Wild boar (Sus scrofa), sika deer (Cervus nippon), and Japanese serow (Capricornis crispus) were photographed only outside the sanitary control zone. In contrast, small and medium-sized mammals, such as feral cats (Felis catus), raccoons (Procyon lotor), and rodent species (Muridae), and birds, such as the rufous oriental turtle dove (Streptopelia orientalis) and crows of the genus Corvus, were photographed both inside and outside the sanitary control zone. This comprehensive quantitative evidence suggests that various mammals and birds may be in indirect contact with livestock, highlighting the need to improve biosecurity at livestock farms. Moreover, while fences are effective against large mammals, they are inefficient against small to medium-sized mammals and birds. Therefore, the results of this study provide important insights into enhancing biosecurity on livestock farms by strengthening physical fencing, disinfecting clothing and equipment, and complying with standards of hygiene management.
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Animales Salvajes , Animales , Japón , Porcinos , Aves , Mamíferos , Sus scrofa , Fotograbar , Crianza de Animales Domésticos/métodos , Crianza de Animales Domésticos/instrumentación , GranjasRESUMEN
The Water-Energy-Food (WEF) nexus approach is increasingly being used for supporting a transition to sustainable development, with initiatives involving the concept of circular economy (CE). In the agricultural sector in particular, assessing this nexus is crucial to ensure food security, control the consumption of key resources such as water and energy, as well as measure atmospheric emissions linked to climate change. This manuscript aims to propose a novel approach by coupling the WEF nexus with a circularity indicator, seeking to capture in a single index (the WEF+CEi) both performances in a sample of companies. The novel approach is applied to 30 dairy farms located in Galicia (NW Spain) to benchmark them in a holistic manner. To do this, the WEF nexus of each farm was represented through the following indicators: carbon footprint, water footprint, energy footprint, and food productivity. In addition, the percentage of circularity for each farm, and for the agro-industrial cooperative was measured thanks to the application of a circularity tool in percentage terms. Finally, the WEF+CEi indicator was obtained using the multicriteria mathematical tool of Data Envelopment Analysis (DEA). The results show that without considering the agro-industrial cooperative, the system is 51 % circular. On the other hand, considering the farms and the cooperative, the system goes up to 80 % of circularity. Finally, the proposed approach can support decision-making and provide insights for producers and stakeholders in the area.
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Benchmarking , Industria Lechera , Industria Lechera/métodos , España , Benchmarking/métodos , Granjas , Huella de Carbono , Agricultura/métodosRESUMEN
Large-scale poultry production in low- and middle-income countries may be a source of adulterated products (e.g., Salmonella contamination, antibiotic residues) that can be disseminated over wide areas. We employed a cross-sectional survey of 199 randomly selected poultry farms in Lagos State, Nigeria, to estimate the prevalence of non-typhoidal Salmonella (NTS), and biosecurity and antibiotic use practices. Pooled fecal samples were collected from laying chickens and from poultry handlers. Selective culture, biochemical assays, and PCR (invA) were used to isolate and confirm NTS isolates. NTS was detected at 14% of farms (28/199) and from 10% of farm workers (6/60). Multivariate logistic regression analysis indicated that antiseptic foot dips reduced the odds ratio (OR) for detecting NTS in chicken feces [OR: 0.55; 95% confidence interval (CI) 0.07-0.58]. Most farms (94.5%, 188/199) used antibiotics for treatment and prophylaxis, but no farms (0/199) exercised withdrawal before sale of products. Most farms (86.4%, 172/199) reported using antibiotic cocktails that included medically important colistin, ciprofloxacin, chloramphenicol, and gentamicin. Egg production in Lagos State relies heavily on antibiotics and antibiotic residues are likely passed to consumers through poultry products, but there is evidence that low-cost biosecurity controls are effective for limiting the presence of NTS on farms.
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Antibacterianos , Pollos , Enfermedades de las Aves de Corral , Salmonella , Animales , Nigeria/epidemiología , Antibacterianos/farmacología , Salmonella/aislamiento & purificación , Salmonella/efectos de los fármacos , Pollos/microbiología , Enfermedades de las Aves de Corral/microbiología , Enfermedades de las Aves de Corral/prevención & control , Estudios Transversales , Salmonelosis Animal/prevención & control , Salmonelosis Animal/epidemiología , Heces/microbiología , Aves de Corral/microbiología , Crianza de Animales Domésticos/métodos , Humanos , Granjas , PrevalenciaRESUMEN
Migratory shorebirds are one of the fastest declining groups of North American avifauna. Yet, relatively little is known about how these species select habitat during migration. We explored the habitat selection of Buff-breasted Sandpipers (Calidris subruficollis) during spring and fall migration through the Texas Coastal Plain, a major stopover region for this species. Using tracking data from 118 birds compiled over 4 years, we found Buff-breasted Sandpipers selected intensively managed crops such as sod and short-stature crop fields, but generally avoided rangeland and areas near trees and shrubs. This work supports prior studies that also indicate the importance of short-stature vegetation for this species. Use of sod and corn varied by season, with birds preferring sod in spring, and avoiding corn when it is tall, but selecting for corn in fall after harvest. This dependence on cropland in the Texas Coastal Plain is contrary to habitat use observed in other parts of their non-breeding range, where rangelands are used extensively. The species' almost complete reliance on a highly specialized crop, sod, at this critical stopover site raises concerns about potential exposure to contaminants as well as questions about whether current management practices are providing suitable conditions for migratory grassland birds.
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Migración Animal , Ecosistema , Estaciones del Año , Animales , Migración Animal/fisiología , Texas , Pradera , Charadriiformes/fisiología , Granjas , Aves/fisiología , Zea maysRESUMEN
Large datasets are required to develop Artificial Intelligence (AI) models in AI powered smart farming for reducing farmers' routine workload, this paper contributes the first large lion-head goose dataset GooseDetectlion, which consists of 2,660 images and 98,111 bounding box annotations. The dataset was collected with 6 cameras deployed in a goose farm in Chenghai district of Shantou city, Guangdong province, China. Images sampled from videos collected during July 9 -10 in 2022 were fully annotated by a team of fifty volunteers. Compared with another 6 well known animal datasets in literature, our dataset has higher capacity and density, which provides a challenging detection benchmark for main stream object detectors. Six state-of-the-art object detectors have been selected to be evaluated on the GooseDetectlion, which includes one two-stage anchor-based detector, three one-stage anchor-based detectors, as well as two one-stage anchor-free detectors. The results suggest that the one-stage anchor-based detector You Only Look Once version 5 (YOLO v5) achieves the best overall performance in terms of detection precision, model size and inference efficiency.
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Inteligencia Artificial , Gansos , Animales , China , GranjasRESUMEN
The cultivation of forage crops on wastewater-irrigated soils, while common in many developing countries, poses significant risks due to heavy metal pollution, particularly Lead (Pb) and Nickel (Ni). This practice, aimed at addressing water scarcity challenges and providing affordable irrigation, was investigated for its ecological and human health implications across three diverse sites (site A, site B, and site C). Our study unveiled increases in Pb concentrations in contaminated soil, cultivated with Sesbania bispinosa showing the highest Pb accumulation. The Ni concentrations ranged from 5.34 to 10.43 across all forage crop samples, with S. fruticosa from site C displaying the highest Ni concentration and S. bicolor from site A exhibiting the lowest. Trace element concentrations in the specimens were determined using an atomic absorption spectrophotometer. The Pb levels in the blood, hair, and feces of farm ruminants (cows, buffaloes, and sheep) varied across the sites, with buffaloes consistently displaying the highest Pb levels. Insights into daily Pb intake by ruminant's highlighted variations influenced by plant species, animal types, and sites, with site C, the cows exhibiting the highest Health Risk Index (HRI) associated with lead exposure from consuming forage crops. Soil and forage samples showed Pb concentrations ranging from 8.003 to 12.29â¯mg/kg and 6.69-10.52â¯mg/kg, respectively, emphasizing the severe health risks associated with continuous sewage usage. Variations in Ni concentrations across animal blood, hair, and feces samples underscored the importance of monitoring Ni exposure in livestock, with sheep at site B consistently showing the highest Ni levels. These findings highlight the necessity of vigilance in monitoring trace element (Pb and Ni) exposure in forage crops and livestock, to mitigate potential health risks associated with their consumption, with variations dependent on species, site, and trace element concentrations.
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Productos Agrícolas , Plomo , Níquel , Contaminantes del Suelo , Níquel/análisis , Níquel/toxicidad , Animales , Contaminantes del Suelo/análisis , Plomo/análisis , Monitoreo del Ambiente , Rumiantes , Ovinos , Bovinos , Suelo/química , GranjasRESUMEN
The present study aimed to classify smallholder dairy production systems by analyzing land allocation for various crop types and characterizing them based on integration with specific crops which is gap in earlier studies. A total of 360 smallholder farmers were interviewed to generate firsthand information in addition to using secondary data sources. The classification utilized K-means clustering, while discriminant analysis was applied to accentuate the distinct characteristics of dairy production systems (DPSs). Subsequently, for a particular crop to be considered dominant, the minimum farmland allocation had to exceed 30%. Based on this, the 360 respondent farmers in the study areas were categorized into four distinct DPSs: diversified crop-based (DCB) at 31%, enset-based (EB) at 28%, cereal-based (CB) at 24% and cash crop-based (CCB) with coffee, khat, vegetables, fruits, and sugarcane accounting for 17%. Within the CCB dairy production system, where cash crops were cultivated most dominantly, averaging 56% of the farmland, farmers reported the highest annual income and, consequently, acquired more improved dairy cows, facilitating their entry into intensive dairy production. Ownership of improved dairy cattle breeds, use of agro-industrial byproducts as feed, practicing stall feeding and better housing are observed practices in this DPS that relate to the intensification of dairy production. This positive relationship was observed between the commercialization of crops and the intensification of dairy production in the CCB dairy production system. In the EB dairy system, enset dominates with a 33% area share, leading to increased residues for cattle feed. They keep large herds of local cattle, limited crossbreeding experience, and prefer conventional feeding. The CB system allocates 47% of land to cereals, justifying cereal residues as primary dairy feed. Cultivating grasses like Pennisetum pedicellatum and Phalaris arundinacea is also highly valued in the system. The DCB system, with diverse crops (none exceeding 30%), implies varied residues for dairy feed. These findings reveal unique characteristics across dairy systems, indicating a positive correlation between crop commercialization and dairy intensification. Hence, understanding each type of DPS and its characteristics will help development experts or those in charge of designing agricultural policy to establish the best strategic plans for enhancing the productivity of dairy cattle under smallholder conditions.
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Productos Agrícolas , Industria Lechera , Etiopía , Industria Lechera/economía , Bovinos , Animales , Productos Agrícolas/crecimiento & desarrollo , Femenino , Agricultores , Agricultura/métodos , Humanos , GranjasRESUMEN
Our understanding of the spread of yeasts in natural ecosystems remains somewhat limited. The recent momentum of yeast ecology research has unveiled novel habitats and vectors that, alongside human activities, impact yeast communities in their natural environments. Yeasts, as non-airborne microorganisms, rely on animal vectors, predominantly insects. However, the overlooked actor in this interplay is the environmental matrix, a player potentially influencing yeast populations and their vectors. This study aims to delve deeper into the intricate, multi-layered connections between yeast populations and ecosystems, focusing on the interactions between the attributes of the environmental matrix, arthropod diversity, and the mycobiota within a renowned yeast-inhabited framework: the vineyard. To investigate these relationships, we sampled both invertebrate and yeast diversity in six organic and conventional vineyards described in terms of management and landscape composition. We identified 80 different invertebrate taxa and isolated 170 yeast strains belonging to 18 species. Notably, new species-specific yeast-insect associations were observed, including the exclusive association between Candida orthopsilosis and Hymenoptera and between Metschnikowia pulcherrima and Coleoptera. These newly identified potential associations provide valuable insights into insect and yeast physiology, hence holding the promise of enhancing our understanding of yeast and arthropod ecology and their collective impact on overall ecosystem health.
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Artrópodos , Biodiversidad , Levaduras , Animales , Artrópodos/microbiología , Levaduras/clasificación , Levaduras/aislamiento & purificación , Levaduras/genética , Ecosistema , GranjasRESUMEN
Improving fattening efficiency is an important goal of breeding commercial pigs, especially for the large-scale pig farms. Fattening efficiency index (FEI) can be used to evaluate the fattening efficiency. The aim of this study was to identify the factors affecting the fattening efficiency of commercial pigs in large-scale pig farms and further study the impact of these factors on the production performance of commercial pig batches at different production levels. The data of 9,570 batches was mainly consisted of four parts (farm facilities, general information of piglets, production performance of nursery pigs and finishing pigs). A total of 28 variables were evaluated by the multi-variable linear regression models. The differences in production factors significantly correlated with FEI at piglets-finishing stage were compared among high-performing (HP), moderate-performing (MP), and low-performing (LP) batches of commercial pigs during the nursery and finishing stage. Among the 28 variables, 18 were significantly correlated with fattening efficiency (P < 0.05), including 11 continuous variables and seven discrete variables. The significant differences among the 11 consecutive variables in the HP, MP, and LP batches of commercial pigs mostly persisted from the piglets-nursery stage to the growing-finishing stage, ultimately affecting the FEI at piglets-finishing stage. For the seven significant discrete variables, the HP batches had a lower proportions in owned source of piglets, number of the purchasing piglets in spring and winter, number of batches in the East and North regions and five-way crossbred pigs, while a higher proportions in the use of closed circuit television video (CCTV) and wastes treatment system. The fattening efficiency of commercial pigs in large-scale pig farms was comprehensively affected by farm facilities, piglets, and production performance at nursery and finishing stage. The low fattening efficiency may have started at the end of nursery stage.
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Crianza de Animales Domésticos , Animales , Porcinos , Crianza de Animales Domésticos/métodos , Granjas , Cruzamiento , Femenino , MasculinoRESUMEN
BACKGROUND: Antimicrobial resistance (AMR) is a significant global health concern, necessitating the monitoring of antimicrobial usage (AMU). However, there is a lack of consensus on the standardized collection and reporting of AMU data in the veterinary field. In Denmark, the Danish Cattle Database (DCDB) contains treatment information on animal level, which allows counting of number of treatments carried out, used daily doses (UDD). The Danish VetStat database (VetStat) contains information on veterinary medicinal prescriptions at farm level and uses fixed standard doses of each product to calculate number of daily treatments, animal daily doses (ADD). This study aimed to compare two different numerators, UDD and ADD, used to describe AMU on Danish cattle farms, and estimate their correlation. RESULTS: Routinely collected registry data from conventional dairy farms in Denmark for 2019 were used, including a total of 2,197 conventional dairy farms. The data from VetStat and the DCDB were aggregated and analysed, and treatment frequencies (TF) were calculated for both UDD and ADD, adjusting for farm size. Spearman correlation analysis and Bland-Altman plots were employed to assess the relationship and agreement between TF for ADD and UDD, respectively. The results showed a high correlation between TF for ADD and UDD for most prescription groups, i.e., groups used to categorise antibiotics based on target organs. An exception is found for the Udder prescription group, where a systematic underreporting of UDD compared to ADD was observed. This discrepancy may be due to combination treatments, and potential missing or grouped registrations in the DCDB. CONCLUSIONS: Our UDD and ADD comparison yields valuable insights on farm-level AMU. We observe strong correlations between UDD and ADD, except for udder treatments, where some farms report only 1/3 UDD compared to ADD, indicating potential underreporting. Further investigations are needed to understand the factors contributing to these patterns and to ensure the accuracy and completeness of recorded information. Standardizing AMU data collection and reporting remains crucial to tackle the global challenge of AMR effectively.
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Antibacterianos , Industria Lechera , Sistema de Registros , Animales , Bovinos , Dinamarca , Femenino , Enfermedades de los Bovinos/tratamiento farmacológico , GranjasRESUMEN
Captive and free-living wildlife serve as significant hosts for Giardia duodenalis. Asiatic black bears, valued for their economic and medicinal importance, are extensively farmed in China and also prevalent in zoos. However, studies on G. duodenalis in these animals in China are limited. Here, 218 feces samples of Asiatic black bears were collected: 36 from a zoo in Heilongjiang Province, and 182 from a farm in Fujian Province. Nested PCR of the SSU rRNA gene, followed by sequencing, was employed to determine the frequency and assemblage distribution of G. duodenalis. Positive samples underwent further analysis through multilocus genotyping (MLG) by amplifying the genes for glutamate dehydrogenase (gdh), ß-giardin (bg), and triosephosphate isomerase (tpi). Of the 218 samples, G. duodenalis was detected in 22 cases at the SSU rRNA gene locus, including three from Heilongjiang and 19 from Fujian. Three assemblages were identified: A (n = 1), B (n = 16), and E (n = 2) in Fujian; and B (n = 3) in Heilongjiang. Out of the 22 positive samples, 20, 19, and 9 were effectively amplified and sequenced across the tpi, gdh, and bg loci, respectively. Seven samples were genotyped successfully at all three loci, identifying MLG-B1 (n = 1), MLG-B2 (n = 1), and MLG-B3 (n = 1), MLG-B4 (n = 1), MLG-B5 (n = 2), and MLG-B6 (n = 1) as the six assemblage B MLGs. This study marks the first documentation of G. duodenalis in Asiatic black bears in captivity in Fujian and Heilongjiang. The identification of zoonotic assemblages A and B, along with E, underscores potential public health concerns.
Title: Prévalence et assemblages de Giardia duodenalis chez les ours noirs d'Asie (Ursus thibetanus) d'élevage et de zoos dans les provinces chinoises du Heilongjiang et du Fujian. Abstract: Les faunes captive et libre incluent des hôtes importants pour Giardia duodenalis. Les ours noirs d'Asie, appréciés pour leur importance économique et médicinale, sont couramment élevés en Chine et répandus dans les zoos. Cependant, les études sur G. duodenalis chez ces animaux en Chine sont limitées. Ici, 218 échantillons d'excréments d'ours noirs d'Asie ont été collectés, 36 dans un zoo de la province du Heilongjiang et 182 dans une ferme de la province du Fujian. La PCR imbriquée de l'ARNr SSU, suivie d'un séquençage, a été utilisée pour déterminer la fréquence et la distribution des assemblages de G. duodenalis. Les échantillons positifs ont subi une analyse plus approfondie par génotypage multilocus (MLG) en amplifiant les gènes de la glutamate déshydrogénase (gdh), de la ß-giardine (bg) et de la triosephosphate isomérase (tpi). Sur les 218 échantillons, G. duodenalis a été détecté dans 22 cas par le locus du gène de l'ARNr SSU, dont trois du Heilongjiang et 19 du Fujian. Trois assemblages ont été identifiés : A (n = 1), B (n = 16) et E (n = 2) dans le Fujian, et B (n = 3) dans le Heilongjiang. Sur les 22 échantillons positifs, 20, 19 et 9 ont été efficacement amplifiés et séquencés respectivement pour les loci tpi, gdh et bg. Sept échantillons ont été génotypés avec succès sur les trois loci, identifiant MLG-B1 (n = 1), MLG-B2 (n = 1) et MLG-B3 (n = 1), MLG-B4 (n = 1), MLG- B5 (n = 2) et MLG-B6 (n = 1) comme les six assemblages MLG B. Cette étude marque la première investigation de G. duodenalis chez les ours noirs d'Asie en captivité au Fujian et au Heilongjiang. L'identification des assemblages zoonotiques A et B, ainsi que E, souligne des problèmes potentiels de santé publique.