RESUMEN

The rev gene product is a trans-acting nuclear regulatory protein that is essential for AIDS virus replication. To define the effect of the SIV rev gene on its gp160 expression, two SV40 constructs have been made: pBC17 is a rev+env+ construct, and pBD21 is a rev-env+ construct. After transfecting the constructs into COS-1 cells, RNA, protein, syncytium formation, and monoclonal antibody blocking assays were performed. The results indicated that the SIV rev gene positively influenced the level of full-length env mRNA and was required for expression of SIV gp160.

Asunto(s)

Regulación Viral de la Expresión Génica , Productos del Gen env/genética , Genes env , Genes rev , Precursores de Proteínas/genética , Virus de la Inmunodeficiencia de los Simios/genética , Anticuerpos Monoclonales , Northern Blotting , Línea Celular , Clonación Molecular , Productos del Gen env/metabolismo , Células Gigantes/metabolismo , Proteínas gp160 de Envoltorio del VIH , Humanos , Plásmidos , Pruebas de Precipitina , Precursores de Proteínas/metabolismo , ARN Mensajero/análisis , ARN Mensajero/genética , ARN Viral/análisis , ARN Viral/genética , Activación Transcripcional , Transfección