RESUMEN
Levan, a polysaccharide that can be produced by both plants and microorganisms, is a sugar polymer composed of fructose, with beta-2,6 linkages. Here, we have attempted to assess the possible use of levan produced by Zymomonas mobilis as a cosmeceutical ingredient. In service of this goal, we assessed a host of levan's properties, including its moisturizing effects, cell cytotoxicity, cell proliferation effects, and anti-inflammation effects. Levan exhibited a moisturizing effect that was almost exactly the same as that evidenced by hyaluronic acid, as well as a similar cell proliferation effect in human fibroblast and keratinocyte cell lines. Moreover, in our cell proliferation test, which was conducted using bio-artificial skin constructed via 3-dimensional (3-D) culture after the induction of primary skin inflammation with 0.05% sodium lauryl sulfate (SLS), cell viability in the presence of levan (0.01 mg/ml, 0.05 mg/ml) was determined to be higher than cell viability in the absence of levan. In our anti-inflammation test, which was also conducted using 3-D artificial skin, and which involved the measurement of a quantity of secreted interleukin-1alpha (IL-1alpha), a pre-inflammatory mediator induced by SLS, we determined that the quantity of IL-1alpha in the 3-D artificial skin treated with 0.01 mg/ml and 0.05 mg/ml of levan was less than that registered in a skin sample that had been treated only with SLS. In this study, we determined that levan exerted an anti-inflammatory effect against inflammatory reactions to skin irritants, and also that levan exerted a cell-proliferative effect in bio-artificial skin, thereby indicating its potential applicability as a cosmeceutical agent.
Asunto(s)
Cosméticos/farmacología , Fructanos/farmacología , Polisacáridos Bacterianos/farmacología , Piel/efectos de los fármacos , Zymomonas/metabolismo , Adulto , Antiinflamatorios/química , Antiinflamatorios/metabolismo , Antiinflamatorios/farmacología , Antiinflamatorios/toxicidad , Secuencia de Carbohidratos , Proliferación Celular/efectos de los fármacos , Cosméticos/química , Cosméticos/metabolismo , Cosméticos/toxicidad , Emolientes/química , Emolientes/metabolismo , Emolientes/farmacología , Emolientes/toxicidad , Femenino , Fibroblastos/citología , Fibroblastos/efectos de los fármacos , Fructanos/química , Fructanos/metabolismo , Fructanos/toxicidad , Humanos , Queratinocitos/citología , Queratinocitos/efectos de los fármacos , Microscopía Electrónica de Transmisión , Datos de Secuencia Molecular , Peso Molecular , Resonancia Magnética Nuclear Biomolecular , Polisacáridos Bacterianos/química , Polisacáridos Bacterianos/metabolismo , Polisacáridos Bacterianos/toxicidad , Piel/metabolismo , Zymomonas/químicaRESUMEN
The effects of cyclophosphamide (CY) and levan at low (0.1 mg) and high (10 mg) doses (previously found to inhibit and stimulate, respectively, B16 melanoma growth) were studied. in single and combined administration, on the blood and bone marrow of C57BL mice. Very marked changes in the cellular composition of blood and bone marrow were induced by the various treatments. CY caused a sharp depletion of leukocytes in blood and bone marrow followed by a restoration of granulocytes while lymphocytes remained low. Levan caused a sharp dose-dependent depletion in bone marrow lymphocytes. Blood lymphocytes were not affected, however, by the polysaccharide. With the high levan dose, after a temporary granulocytopenia and monocytopenia, prominent granulocytosis was observed. While CY affected equally the different types of cells, levan decreased the number of lymphocytes and erythroblasts to a greater degree than the number of granulocytes. In the combined CY-levan treatments, the low levan dose generally attenuated the suppressive effect of CY, while the high concentration aggravated it.