RESUMEN
BACKGROUND: Favism is an acute hemolytic syndrome caused by fava bean (FB) ingestion. The purpose of this study was to investigate the possible influences of FB on the metabonomic profile of erythrocytes in glucose-6-phosphate dehydrogenase (G6PD)-deficient (G6PDx) and wild-type (WT) mice. RESULTS: Ninety-two metabolites were identified in the comparison of the G6PDx and WT groups. Eighty-seven metabolites were identified in the erythrocytes of WT and G6PDx mice after FB ingestion. Thirty-eight metabolites were identified in the comparison of the FB-treated G6PDx and the FB-treated WT mouse groups. Among them, the number of glycerophospholipids (GPLs) and polyunsaturated fatty acids (PUFAs) changed significantly, which suggests that GPLs and PUFAs may be responsible for FB stress. CONCLUSION: This study demonstrates that G6PD deficiency might affect the metabonomic profile of erythrocytes in response to FB. © 2020 Society of Chemical Industry.
Asunto(s)
Eritrocitos/metabolismo , Favismo/metabolismo , Deficiencia de Glucosafosfato Deshidrogenasa/metabolismo , Vicia faba/metabolismo , Animales , Eritrocitos/enzimología , Ácidos Grasos Insaturados/metabolismo , Favismo/enzimología , Favismo/genética , Deficiencia de Glucosafosfato Deshidrogenasa/enzimología , Deficiencia de Glucosafosfato Deshidrogenasa/genética , Glicerofosfolípidos/metabolismo , Humanos , Masculino , Metabolómica , Ratones , Ratones Endogámicos C3H , Ratones NoqueadosRESUMEN
BACKGROUND: Favism occurs as the result of intolerance to the ingesting of fava beans or to the inhalation of pollen from the Vicia faba plant. Patients with favism are always Glucose-6-phosphate dehydrogenase (G6PD)-deficient, but not all G6PD-deficient individuals develop hemolysis as a result of fava beans consumption. PATIENTS AND METHODS: Blood samples were collected from children with favism (n = 55) between age (2-12 years) on EDTA tubes divided into 3 groups: group 1 control group (n = 15), group 2 before blood transfusion (during hemolytic action) (n = 20) and group 3 after blood transfusion (treated) (n = 20). RESULTS: It was found that in group 2 GSH level was significantly low; (1.11 ± 0.39, p < 0.001) compared to controls (26.31 ± 5.26, p < 0.001). In group 3 after blood transfusion Level of GSH rose but remained lower than normal level (5.88 ± 2.33, p < 0.001) compared to controls. As for oxidative stress parameters, both levels of H2O2 and MDA were highly significant in group 2; (213.49 ± 57.56, p < 0.001), (98.05 ± 22.34, p < 0.001) compared to controls (3.75 ± 1.164, p < 0.001), (7.38 ± 2.07, p < 0.001), respectively. Moreover, in group 3 after blood transfusion, levels of H2O2 and MDA were decreased but remained high compared to controls (66.55 ± 22.49, p < 0.001), (47.18 ± 9.62, p < 0.001) sequentially. Also, there was a negative correlation between GSH that acts as antioxidant defense enzyme and each one of oxidative stress parameters MDA & H2O2. However, there was a positive correlation between H2O2 and MDA. CONCLUSIONS: From this study, it could be concluded that the favic patients have high oxidative stress (H2O2 and MDA) more than normal individuals and less antioxidant defense (GSH). With the passage of time these individuals, cells would be more vulnerable for H2O2-induced senescence.