RESUMEN
In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol from S. alexandrina (aerial parts). The three UAE factors, extraction temperature (S1), extraction time (S2), and liquid to solid ratio (S3), were optimized using response surface methodology (RSM). A Box-Behnken design was used for experimental design and phytoconstituent analysis was performed using high-performance liquid chromatography-UV. The optimal extraction conditions were found to be a 64.2 °C extraction temperature, 52.1 min extraction time, and 25.2 mL/g liquid to solid ratio. The experimental values of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol (2.237, 12.792, 2.457, 0.261, and 1.529%, respectively) agreed with those predicted (2.152, 12.031, 2.331, 0.214, and 1.411%, respectively) by RSM models, thus demonstrating the appropriateness of the model used and the accomplishment of RSM in optimizing the extraction conditions. Excellent antioxidant properties were exhibited by S. alexandrina methanol extract obtained using the optimized extraction conditions with a DPPH assay (IC50 = 59.7 ± 1.93, µg/mL) and ABTS method (47.2 ± 1.40, µg/mL) compared to standard ascorbic acid.
Asunto(s)
Antioxidantes/farmacología , Fraccionamiento Químico/métodos , Componentes Aéreos de las Plantas/química , Extracto de Senna/farmacología , Senna/química , Sonicación , Ondas Ultrasónicas , Algoritmos , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Modelos Teóricos , Estructura Molecular , Fitoquímicos , Extracto de Senna/química , Extracto de Senna/aislamiento & purificaciónRESUMEN
Cassia fistula L. which is known as "Golden Shower", is used as an ornamental plant due to its flowers, and fruit parts of this plant have a high medicinal value. There are few reports providing a comprehensive overview of the chemical composition of its fruit or explaining the differences between samples from different sources because of the complexity of its chemical components. The purpose of the present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) for the composition identification and quality control of this herb. Twelve samples from Xinjiang and Sichuan provinces in China and India were analyzed by HPLC, and there were fifteen common peaks in the twelve batches. Molecular weight and formula information can be derived from thirty-one peaks by UHPLC/LTQ-Orbitrap MSn, molecular structure information of twenty components was obtained, of which ten compounds were identified by comparison with standard materials. Samples of twelve batches were divided according to their similarity into four groups, which were basically consistent with three different C.fistula fruit-producing areas. Five compounds were finally considered to be chemical markers to determine the quality of this herb. A fingerprints method combined with chemometrics was established to differentiate the origin of the fruit of C. fistula which has the advantages of effectivity and convenience, laying the foundation for the quality evaluation of this herb from different sources.
Asunto(s)
Cassia/química , Medicamentos Herbarios Chinos/química , Frutas/química , Metaboloma , China , Cromatografía Líquida de Alta Presión , Análisis por Conglomerados , Flavanonas/química , Flavanonas/aislamiento & purificación , Quempferoles/química , Quempferoles/aislamiento & purificación , Estructura Molecular , Análisis de Componente Principal , Extracto de Senna/química , Extracto de Senna/aislamiento & purificaciónRESUMEN
Senna is an important medicinal plant and is used in many Ayurvedic formulations. Dianthraquinone glucosides are the main bioactive phytochemicals present in leaves and pods of senna. The extraction efficiency in terms of yield and composition of the extract of senna prepared using both conventional (cold percolation at room temperature and refluxing) and non conventional (ultrasound and microwave assisted solvent extraction as well as supercritical fluid extraction) techniques were compared in the present study. Also a rapid reverse phase HPLC-PDA detection method was developed and validated for the simultaneous determination of sennoside A and sennoside B in the different extracts of senna leaves. Ultrasound and microwave assisted solvent extraction techniques were more effective in terms of yield and composition of the extracts compared to cold percolation at room temperature and refluxing methods of extraction.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Extracto de Senna/aislamiento & purificación , Senna/química , Cromatografía de Fase Inversa , Cromatografía con Fluido Supercrítico , Hojas de la Planta/química , Extracto de Senna/análisis , SenósidosRESUMEN
OBJECTIVE: The aim of this study was to develop a method of removing sennoside to reduce the cathartic effect of rhubarb while conserving its other pharmacological activities. METHODS: Rhubarb powder was steam autoclaved at 121°C and 0.14 MPa for 20, 60, or 120 minutes, and HPLC analysis was conducted to determine levels of rhubarb components. Mice were fed non-autoclaved or 20-minute-autoclaved rhubarb extracts. Feces were collected and weighed over a 24-hour period. India ink was orally administered to determine the distance of fecal migration through the intestinal tract. RESULTS: Autoclaving 20, 60, and 120 minutes decreased sennoside A and B to trace levels but only autoclaving 20 minutes conserved most of the (+)-catechin, (-)-epicatechin, and (-)-epicatechin gallate contents (i.e., 69%, 90%, 88%, respectively). Therefore only rhubarb autoclaved for 20 minutes was used in subsequent experiments. Fecal output (in g) in mice treated with water (control), autoclaved rhubarb, and non-autoclaved rhubarb was 2.78 ± 0.07, 3.30 ± 0.13 (p = 0.348), and 3.81 ± 0.07 (p = 0.005). India ink migration was far less in mice treated with autoclaved rhubarb vs non-autoclaved rhubarb. CONCLUSION: Steam autoclaving the rhubarb for 20 minutes reduces sennoside levels and its cathartic activity while conserving its other pharmacological activities.
Asunto(s)
Catárticos/aislamiento & purificación , Catárticos/farmacología , Composición de Medicamentos/métodos , Extractos Vegetales/química , Rheum/química , Extracto de Senna/aislamiento & purificación , Extracto de Senna/farmacología , Animales , Catárticos/farmacocinética , Cromatografía Líquida de Alta Presión/métodos , Calor , Ratones Endogámicos C57BL , Extracto de Senna/farmacocinética , Senósidos , Vapor , Factores de TiempoRESUMEN
Context Plants and plant products have been used in traditional medicine as anthelmintic agents in human and veterinary medicine. Three species of Senna plant, S. alata (L), S. alexandrina (M) and S. occidentalis (L.) Link (Fabaceae) have been shown to have a vermicidal/vermifugal effect on a zoonotic tapeworm Hymenolepis diminuta (Rudolphi) (Cyclophyllidean). Objective The present study validates the mode of action of these Senna plants on the parasite. The alcoholic leaf extract was determined to obtain information on the intracellular free calcium concentration level. Materials and methods Hymenolepis diminuta was maintained in Sprague-Dawley rat model for 2 months. Live parasites collected from infected rat intestine were exposed to 40 mg/mL concentration of each plant extracts prepared in phosphate buffer saline at 37 °C, till parasite gets paralyzed. The rate of efflux of calcium from the parasite tissue to the medium and the level of intracellular Ca(2+ )concentration were determined by an atomic absorption spectroscopy. Results This study revealed that exposure of the worms to the plant extract leads to disruption in intracellular calcium homeostasis. A significant increase (44.6% and 25%) of efflux in Ca(2+ )from the tissue to the incubated medium was observed. Senna alata showed high rate of efflux (5.32 mg/g) followed by S. alexandria and S. occidentalis (both 4.6 mg/g) compared with control (3.68 mg/g). Discussion and conclusion These results suggest that leaf extracts caused membrane permeability to Ca(2+ )after vacuolization of the tegument under stress and the extracts may contain compound that can be used as a chemotherapeutic agent.
Asunto(s)
Anticestodos/farmacología , Calcio/metabolismo , Himenolepiasis/tratamiento farmacológico , Hymenolepis diminuta/efectos de los fármacos , Intestinos/microbiología , Extracto de Senna/farmacología , Senna , Animales , Anticestodos/aislamiento & purificación , Modelos Animales de Enfermedad , Homeostasis , Himenolepiasis/parasitología , Himenolepiasis/transmisión , Hymenolepis diminuta/crecimiento & desarrollo , Hymenolepis diminuta/metabolismo , Fitoterapia , Plantas Medicinales , Ratas Sprague-Dawley , Extracto de Senna/aislamiento & purificación , Senna/química , Factores de TiempoRESUMEN
Senna leaf is widely consumed as tea to treat constipation or to aid in weight loss. Sennoside A, A1 , and B are dirheinanthrone glucosides that are abundant and the bioactive constituents in the plant. They are isomers that refer to the (R*R*), (S*S*), and (R*S*) forms of protons on C-10 and C-10' centers and it is difficult to refine them individually due to their structural similarities. The new separation method using counter-current chromatography successfully purified sennoside A, A1 , and B from senna leaf (Cassia acutifolia) while reversed-phase medium-pressure liquid chromatography yielded sennoside A only. n-Butanol/isopropanol/water (5:1:6, v/v/v) was selected as the solvent system for counter-current chromatography operation, and the partition coefficients were carefully determined by adding different concentrations of formic acid. High-resolution mass spectrometry and NMR spectroscopy were performed to verify the chemical properties of the compounds.
Asunto(s)
Distribución en Contracorriente , Hojas de la Planta/química , Extracto de Senna/química , Extracto de Senna/aislamiento & purificación , Senna/química , Conformación Molecular , EstereoisomerismoRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Aquaporin-3 (AQP3) is expressed in mucosal epithelial cells in the colon and is important for regulating fecal water content. We examined the role of AQP3 in the laxative effect of rhubarb extract. METHODS: After orally administering rhubarb extract or its major component (sennoside A) to rats, the fecal water content, AQP3 expression and prostaglandin E2 (PGE2) concentrations in the colon were examined. The mechanism by which sennoside A decreases the expression of AQP3 was examined using the human colon cancer HT-29 cells and macrophage-derived Raw264.7 cells. RESULTS: During diarrhea by rhubarb extract administration, the PGE2 levels in the colon increased while the AQP3 expression significantly decreased. Similar changes were also observed when sennoside A was administered. When sennoside A or its metabolites, rheinanthrone and rhein were added to Raw264.7 cells, a significant increase in the PGE2 concentration was observed only in cells treated with rheinanthrone. Fifteen minutes after adding PGE2 to the HT-29 cells, the AQP3 expression decreased to approximately 40% of the control. When pretreated with indomethacin, sennoside A neither decreased the AQP3 expression nor induced diarrhea. CONCLUSIONS: Sennoside A may decrease AQP3 expression in the colon to inhibit water transport from the luminal to the vascular side, leading to a laxative effect. The decreases in the levels of AQP3 are caused by rheinanthrone, which is a metabolite of sennoside A, this metabolite activates the macrophages in the colon and increases the secretion of PGE2; PGE2 acts as a paracrine factor and decreases AQP3 expression in colon mucosal epithelial cells.
Asunto(s)
Acuaporina 3/metabolismo , Laxativos/farmacología , Rheum/química , Extracto de Senna/farmacología , Animales , Antracenos/aislamiento & purificación , Antracenos/farmacología , Línea Celular , Colon/efectos de los fármacos , Colon/metabolismo , Neoplasias del Colon/metabolismo , Diarrea/inducido químicamente , Dinoprostona/metabolismo , Células HT29 , Humanos , Indometacina/farmacología , Mucosa Intestinal/efectos de los fármacos , Mucosa Intestinal/metabolismo , Laxativos/aislamiento & purificación , Activación de Macrófagos/efectos de los fármacos , Masculino , Ratas , Ratas Wistar , Extracto de Senna/aislamiento & purificación , SenósidosRESUMEN
OBJECTIVE: To investigate the influences of Changan Granule on the model of rat with irritable bowel syndrome (IBS) by observing the weight, the content of Nitric Oxide (NO) and 5-hydroxytryptamine (5-HT) in serum of rat. METHODS: The model of IBS rat was established by decoction of Folium Sennae and restraint stress, effects of Changan Granule were studied through observing the influences on rat weight and the content of NO and 5-HT in serum. RESULTS: Compared to those of the model group, Changan Granule could improve diarrhea induced by Folium Sennae and increase body weight obviously (P<0.05 or P<0.01). Compared to the control group, Changan Granule could also increase the content of NO and 5-HT in serum significantly (P<0.05 or P<0.01). Through histological survey, we discovered that the high and middle dose of Changan Granule could change the symptom of inflammation and improve the morphous of epithelium mucosae compared to the model group. And there were no obvious pathological changes on the high and middle dose groups of Changan Granule compared to the control group. CONCLUSION: Changan Granule is effective to cure IBS and has protective effects on the bowel mucosae.
Asunto(s)
Medicamentos Herbarios Chinos/uso terapéutico , Síndrome del Colon Irritable/tratamiento farmacológico , Óxido Nítrico/sangre , Fitoterapia , Plantas Medicinales/química , Serotonina/sangre , Animales , Peso Corporal/efectos de los fármacos , Diarrea/tratamiento farmacológico , Diarrea/etiología , Diarrea/patología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Combinación de Medicamentos , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/farmacología , Femenino , Mucosa Intestinal/efectos de los fármacos , Síndrome del Colon Irritable/sangre , Síndrome del Colon Irritable/etiología , Síndrome del Colon Irritable/patología , Masculino , Distribución Aleatoria , Ratas , Ratas Wistar , Extracto de Senna/aislamiento & purificaciónRESUMEN
The hydroalcoholic extract of Tinnevelli senna is widely used as a laxative phytomedicine. In order to improve the knowledge of the chemical composition of this extract, LC/MS and LC/MS(n) studies were performed, allowing the on-line identification of most of the known constituents, i. e., flavonoids, anthraquinones and the typical dianthronic sennosides. However, the identity of four compounds could not be ascertained on-line under the given LC/MS conditions. These substances were isolated and their structures elucidated as kaempferol, the naphthalene derivative tinnevellin 8-glucoside and two new carboxylated benzophenone glucosides.
Asunto(s)
Benzofenonas/aislamiento & purificación , Flavonoides , Glucósidos/aislamiento & purificación , Quempferoles , Naftalenos/aislamiento & purificación , Quercetina/análogos & derivados , Extracto de Senna/aislamiento & purificación , Senna , Benzofenonas/química , Cromatografía Liquida/métodos , Glucósidos/química , Glucósidos/farmacología , Espectrometría de Masas/métodos , Naftalenos/química , Naftalenos/farmacología , Estructuras de las Plantas/química , Quercetina/química , Quercetina/aislamiento & purificación , Extracto de Senna/químicaRESUMEN
Senna is a well-known drug, used in the Ayurvedic and Allopathic systems of medicine, and is a treatment for constipation. The purgative action of senna and its formulations is due to the presence of sennosides A and B. An HPTLC method has been developed for the determination of individual sennosides (A, B, C, D) without any derivatization in marketed formulations (three tablet formulations, two granule formulations and one liquid formulation) and plant materials (senna leaf and pod). The methanolic solution of a sample was applied on a pre-coated silica gel G60 F254 TLC plate (E. Merck.) and was developed using n-propanol : ethyl acetate : water : glacial acetic acid (3 : 3 : 2 : 0.1 v/v) as the mobile phase. The relative band speeds (Rf values) obtained were 0.35, 0.25, 0.61, 0.46 for sennosides A, B, C and D, respectively. The densitometric response was monitored at 366nm. Calibration curves were found to be linear in the concentration ranges 193-1356, 402-2817, 71-497 and 132-927 ng per spot for sennosides A, B, C, and D, respectively. The correlation coefficients were found to be 0.9978, 0.9987, 0.9939 and 0.9983 respectively for sennosides A, B, C and D. The result obtained with the HPTLC method for total sennoside content was compared with the results using the pharmacopoeial methods (spectrophotometric (British Pharmacopoeia) and spectrofluorimetric (United States Pharmacopeia) using the 'F' test). The results revealed no significant difference in the three different methods for estimation of total sennoside. The proposed HPTLC method was found to be simple, specific, precise, accurate and rapid. It can be used for routine quality control of sennosides or senna-containing formulations for individual sennosides.
Asunto(s)
Antraquinonas/análisis , Cassia/química , Catárticos/análisis , Cromatografía en Capa Delgada/métodos , Plantas Medicinales , Antraquinonas/aislamiento & purificación , Hojas de la Planta/química , Reproducibilidad de los Resultados , Semillas/química , Extracto de Senna/análisis , Extracto de Senna/aislamiento & purificación , Senósidos , Sensibilidad y EspecificidadRESUMEN
The optical isomer of sennoside A--called sennoside A1--postulated by Stoll already in 1950--was isolated from senna pods. Structure identification was carried out by TLC, HPLC, UV, CD and MS.