RESUMEN
Pesticides and veterinary drugs are widely employed to support food production. Assessing potential risks associated with the dietary consumption of pesticide and veterinary drug residues is, however, essential. Potential risks depend on the toxicity degree of the analyzed residue and population exposure levels. Human populations are exposed to numerous chemical substances through different pathways with varying exposure times, leading to increased health risks when compared to exposure to individual chemicals. Cumulative exposure assessments usually assess combined exposures to multiple chemicals through multiple exposure pathways. In this sense, this comprehensive review aims to provide insights into cumulative dietary pesticide and veterinary drug residue exposures. The main methodologies, strategies, and legislation employed by international agencies to this end are discussed. A review concerning articles that apply existing methodologies and approaches, as well as the challenges in this context faced by Brazil is also presented. As this is a critical issue not only for Brazilian public health but also for the global community, regulatory agencies should prioritize formulating regulations that incorporate exposure assessments regarding the simultaneous presence of residues and contaminants in foodstuffs.
Asunto(s)
Exposición Dietética , Contaminación de Alimentos , Plaguicidas , Drogas Veterinarias , Brasil , Humanos , Exposición Dietética/análisis , Drogas Veterinarias/análisis , Contaminación de Alimentos/análisis , Plaguicidas/análisis , Animales , Medición de Riesgo , Residuos de Plaguicidas/análisisRESUMEN
Bakery products, including biscuits, cakes and breads, generally present a high content of simple sugars of rapid absorption, high fat content and low amount of dietary fiber, which make them highly caloric foods. Although sucrose is a very important ingredient in bakery products for its preservation characteristics and a significant source of energy, there is a growing interest in replacing this sugar with alternative substances, such as high-intensity sweeteners (HIS) that provide sweetness with no or low calories. In Brazil, there is no data on the use of HIS in this class of food. Therefore, the objective of this study was to evaluate the presence of HIS in baked food commercially available in the country and estimate the dietary exposure to these food additives. For that, an analytical method was established for the simultaneous determination of nine HIS in bakery products using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). Sample preparation steps were required based on mechanical kneading for homogenization, hexane extraction of fats, dilution in mobile phase and vortex homogenization, prior to injection into the system. The results obtained during validation showed that coefficients of variation (CV%) for precision were lower than 13.8% and the accuracy was between 91.6% and 109.1%. Aspartame, acesulfame potassium, sodium cyclamate, saccharin, sucralose and steviol glycosides were found in the samples, used alone or in combinations of up five substances. Steviol glycosides were the most found HIS in biscuit samples, while sucralose was the most common sweetener in cake and bread samples. Analysis of product labels revealed only three different claims, .i.e. 'no sugar', 'no added sugar' and 'zero sugar', with the latter being found in 70% of the samples. Exposure to HIS through the consumption of bakery products estimated per eating occasion showed no concerns regarding toxicological risk.
Asunto(s)
Exposición Dietética , Edulcorantes , Espectrometría de Masas en Tándem , Edulcorantes/análisis , Brasil , Humanos , Exposición Dietética/análisis , Cromatografía Líquida de Alta Presión , Análisis de los Alimentos , Pan/análisisRESUMEN
Ochratoxin A (OTA), zearalenone (ZEN), and deoxynivalenol (DON) are mycotoxins whose exposure is associated with various adverse health effects, including cancer and renal disorders, estrogenic effects, and immunosuppressive and gastrointestinal disorders, respectively. Infants (<2 years) are the most vulnerable group to mycotoxins, representing a unique combination of restricted food consumption types, low body weight, lower ability to eliminate toxins, and more future years to accumulate toxins. This study aimed to estimate the infantÌs exposure to OTA, DON, and ZEN due to the consumption of milk formula and baby cereals in Chile. Milk formula samples (n = 41) and baby cereals (n = 30) were collected and analyzed using commercial ELISA kits for OTA, DON, and ZEA determination. Exposure was assessed by the Estimated Daily Intake (EDI) approach (mean and worst-case scenario, WCS) with the levels found in a modified Lower Bound (mLB) and Upper Bound (UB); ideal consumption (<6m, 7-12 m, and 13-24 m); adjusted by the weight of each group. The risk was estimated by comparing the EDI with a reference tolerable daily intake or by the margin of exposure (MOE) in the case of OTA. DON and OTA occurrence in infant formula were 34 % and 41 %, respectively. The co-occurrence between these mycotoxins was 22 %. Mycotoxin contents were below LOQ values except for OTA determined in one sample (0.29 ng/ml). No milk formulae were contaminated with ZEN. In the case of baby cereals, the occurrences were 17 % for OTA, 30 % for DON, and 7 % for ZEN, all below LOQ. Co-occurrence was seen in two samples between ZEN and OTA. According to exposure calculations, the MOE for OTA was less than 10,000 in all models for milk formula between 0 to 12 months of age and in the UB and WCS for cereal consumption. Health concerns were observed for DON in the WCS and UB for milk consumption in all ages and only in the UB WCS for cereal consumption. Considering the high consumption of milk formula in these age groups, regulation of OTA and other co-occurring mycotoxins in infant milk and food is strongly suggested.
Asunto(s)
Exposición Dietética , Grano Comestible , Contaminación de Alimentos , Fórmulas Infantiles , Ocratoxinas , Tricotecenos , Zearalenona , Humanos , Zearalenona/análisis , Fórmulas Infantiles/química , Chile , Grano Comestible/química , Lactante , Tricotecenos/análisis , Contaminación de Alimentos/análisis , Ocratoxinas/análisis , Exposición Dietética/análisis , Exposición Dietética/efectos adversos , Medición de Riesgo , Recién Nacido , Alimentos Infantiles/análisisRESUMEN
Previous studies on university students have indicated a significant decline in the consumption of fruits and vegetables complemented by an increase in energy-dense foods. The food toxicant, acrylamide, typically occurs in carbohydrate-rich, energy-dense foods that have been heated. Hence, this work presents an estimated dietary acrylamide exposure for university students in Trinidad and Tobago. A 2-day dietary recall method was used to obtain the food consumption information from 683 university students of differing sociodemographic backgrounds. The acrylamide exposure was estimated using a deterministic approach. The median acrylamide intake was estimated to be 1.39 µg/kg bw/day. The estimated mean acrylamide dietary intakes for the female and male population were 1.40 and 1.37 µg/kg bw/day, respectively. Coffee was determined to be the major dietary contributor to acrylamide exposure. However, bread was the food item that was most frequently consumed among the students. Using multiple linear regression, a possible correlation was detected between the acrylamide exposure and these variables: dietary habits (mostly eat out; p < 0.05), and Indian ethnicity (p < 0.10). Using the margin of exposure approach, dietary acrylamide exposure was found to be a health concern with regards to neurotoxicity and carcinogenicity. An evaluation of the procedures and results from this pilot study was carried out for the potential of conducting a full-scale research project.
Asunto(s)
Acrilamida , Dieta , Estudiantes , Humanos , Acrilamida/análisis , Proyectos Piloto , Femenino , Masculino , Trinidad y Tobago , Universidades , Adulto Joven , Adulto , Contaminación de Alimentos/análisis , Exposición Dietética/análisis , AdolescenteRESUMEN
Infant cereals, one of the first solid foods introduced to infants, have been reported to pose risks to human health because they contain toxic elements and an excess of essential elements. The objective of this study was to assess the cancer and non-cancer risk of exposure to essential and toxic elements in infant cereal in Brazil. In our analyses, we included data from 18 samples of infant cereals made from different raw materials and estimated the incremental lifetime cancer risks and non-cancer hazard quotients (HQs) for their consumption. Rice cereal is particularly concerning because it is immensely popular and usually contains high levels of inorganic arsenic. In addition to arsenic, we assessed aluminum, boron, barium, cadmium, chromium, copper, lead, manganese, nickel, selenium, silver, strontium, and zinc. The cancer risk was highest for rice cereal, which was also found to have an HQ > 1 for most of the tested elements. Inorganic As was the element associated with the highest cancer risk in infant cereal. All of the infant cereals included in this research contained at least one element with an HQ > 1. The essential and non-essential elements that presented HQ > 1 more frequently were zinc and cadmium, respectively. The cancer and non-cancer risks could potentially be decreased by reducing the amount of toxic and essential elements (when in excess), and public policies could have a positive influence on risk management in this complex scenario.
Asunto(s)
Grano Comestible , Brasil , Medición de Riesgo , Humanos , Grano Comestible/química , Lactante , Alimentos Infantiles/análisis , Contaminación de Alimentos/análisis , Exposición Dietética/análisis , Oligoelementos/análisis , Oligoelementos/toxicidad , Arsénico/análisis , Arsénico/toxicidad , Neoplasias/epidemiología , Neoplasias/inducido químicamenteRESUMEN
In the current study, the occupational and dietary exposures of feed handling workers (N = 28) to aflatoxins (AFs), fumonisins (FBs), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), toxins T-2 and HT-2 were assessed for the first time in animal-producing farms and feed factories from São Paulo, Brazil. Mycotoxins in food (n = 244) and airborne dust (n = 27), as well as biomarkers in urine (n = 97) samples were determined by liquid chromatography coupled with tandem mass spectrometry. FBs were detected in all airborne dust samples, with concentrations ranging from 7.85 to 16,839 ng/m3. The mean probable daily intake (PDI) based on food data were 0.005, 0.769, 0.673 and 0.012 µg/kg of body weight (bw)/day for AFs, FBs, DON and ZEN, respectively. Mean PDI values obtained through urinary biomarkers were 0.29, 0.10, 0.50, 9.72 and 0.10 µg/kg body weight/day for AFB1, DON, OTA, FB1 and ZEN, respectively. The analyses based on urinary biomarkers revealed a potential health concern for OTA and FBs, although no potential health concern was observed with PDI calculated through food data. Results of this trial stress the need for preventive measures to avoid health risks of workers in Brazilian animal-producing farms and feed industries.
Asunto(s)
Exposición Dietética , Manipulación de Alimentos , Micotoxinas , Exposición Profesional , Biomarcadores/orina , Peso Corporal , Brasil , Exposición Dietética/análisis , Polvo/análisis , Contaminación de Alimentos/análisis , Humanos , Micotoxinas/análisis , Exposición Profesional/análisis , Salud Rural , Zearalenona/análisisRESUMEN
The aim of this study was to conduct a first evaluation on the co-occurrence of aflatoxins (AF) M1, B1, B2, G1 and G2; fumonisins (F) B1 and B2; deoxynivalenol (DON); de-epoxydeoxinivalenol (DOM-1); ochratoxin A (OTA); zearalenone (ZEN); α-zearalenol (α-ZEL); and ß-zearalenol (ß-ZEL) in 68 samples of fluid milk consumed in Pirassununga, São Paulo, Brazil. The probable daily intake (PDI) was also calculated for each mycotoxin evaluated. Mycotoxins were determined by liquid chromatography coupled to mass spectrometry. Sixty-two (91.2%) samples contained at least one type of mycotoxin. AFM1 was found in 6 samples (8.8%), and none of them presented concentrations above the Brazilian maximum permitted level in milk (500 ng/L). Low levels of non-regulated mycotoxins DOM-1, OTA, FB1, FB2, α-ZEL and ß-ZEL were found in 6 (8.8%), 17 (25%), 10 (14.7%), 3 (4.4%), 39 (57.4%) and 28 (41.2%) samples of milk, respectively. None of the PDIs calculated for the quantified mycotoxins were above recommended values, indicating low exposure through milk consumption in the area studied. However, 21 samples (30.9%) contained 2-4 types of mycotoxins, which warrants concern about the potential adverse effects of mycotoxin mixtures in milks.
Asunto(s)
Leche/química , Micotoxinas/análisis , Adulto , Animales , Brasil , Preescolar , Exposición Dietética/análisis , Contaminación de Alimentos/análisis , Humanos , Proyectos PilotoRESUMEN
Human are exposed to a wide range of mycotoxins through dietary food intake, including processed food. Even most of the mycotoxin exposure assessment studies are based on analysis of foodstuffs, and evaluation of dietary intake through food consumption patterns and human biomonitoring methods are rising as a reliable alternative to approach the individual exposures, overcoming the limitations of the indirect dietary assessment. In this study, human urine samples were analyzed, seeking the presence of deoxynivalenol (DON), ochratoxin A (OTA), zearalenone (ZEA), and their metabolites. For this purpose, 40 urine samples from female and male adult residents in the city of Valencia (Spain) were evaluated by liquid chromatography quadrupole time-of-flight mass spectrometry (LC-ESI-qTOF) after salting-out liquid-liquid extraction. Analytical data showed that 72.5% of analyzed samples were contaminated by at least one mycotoxin at variable levels. The most prevalent mycotoxins were de-epoxy DON (DOM-1) (53%), ZEA (40%), and α-zearalenol (αZOL) (43%), while OTA was only detected in one sample. The mean concentrations in positive samples were DON (9.07 ng/mL), DOM-1 (20.28 ng/mL), ZEA (6.70 ng/mL), ZEA-14 glucoside (ZEA-14-Glc) (12.43 ng/mL), αZOL (27.44 ng/mL), αZOL-14 glucoside (αZOL-14-Glc) (12.84 ng/mL), and OTA (11.73 ng/mL). Finally, probable daily intakes (PDIs) were calculated and compared with the established tolerable daily intakes (TDIs) to estimate the potential risk of exposure to the studied mycotoxins. The calculated PDI was below the TDI value established for DON in both female and male adults, reaching a percentage up to 30%; however, this percentage increased up to 92% considering total DON (DON + DOM-1). On the other hand, the PDI obtained for ZEA and its metabolites were higher than the TDI value fixed, but the low urine excretion rate (10%) considered should be highlighted. Finally, the PDI calculated in the detected positive sample for OTA exceeded the TDI value. The findings of the present study confirm the presence of the studied mycotoxins and their metabolites as some of the most prevalent in urine.
Asunto(s)
Exposición Dietética/análisis , Ocratoxinas/orina , Tricotecenos/orina , Zearalenona/orina , Adolescente , Adulto , Anciano , Monitoreo Biológico , Biomarcadores/orina , Cromatografía Liquida , Femenino , Humanos , Masculino , Persona de Mediana Edad , Medición de Riesgo , Espectrometría de Masa por Ionización de Electrospray , Adulto JovenRESUMEN
The present study investigated the presence of 30 mycotoxins in 110 beverage samples of beer, wine, cava, and cider purchased in Valencia (Spain). A validated method based on dispersive liquid-liquid microextraction and chromatographic methods coupled with tandem mass spectrometry was applied. The method showed satisfactory recoveries ranging from 61 to 116% for the different beverages studied. The detection and quantification limits ranged from 0.03 to 2.34 µg/L and 0.1 to 7.81 µg/L, respectively. The results showed that beer samples were the most contaminated, even with concentrations ranging from 0.24 to 54.76 µg/L. A significant presence of alternariol was found in wine, which reached concentrations up to 26.86 µg/L. Patulin and ochratoxin A were the most frequently detected mycotoxins in cava and cider samples, with incidences of 40% and 26%, respectively. Ochratoxin A exceeded the maximum level set by the EU in one wine sample. The results obtained were statistically validated. The combined exposure was assessed by the sum of mycotoxin concentrations contaminating the same samples to provide information on the extent of dietary exposure to mycotoxins. No significant health risk to consumers was associated with the mycotoxin levels detected in the beverages tested.
Asunto(s)
Bebidas/análisis , Exposición Dietética/análisis , Micotoxinas/análisis , Adulto , Monitoreo del Ambiente , Contaminación de Alimentos/análisis , Humanos , Medición de Riesgo , EspañaRESUMEN
A liquid chromatography tandem mass spectrometry method (LC-MS/MS) preceded by a rapid and simple QuEChERS-based sample preparation was developed and validated for the determination of acrylamide in non-centrifugal sugar (NCS), a solid product obtained from sugarcane juice without centrifugation, up to a concentration greater than 90 ºBrix. Adequate linearity in calibration curves, analytical selectivity and sensitivity were achieved. A limit of detection (LOD) and a limit of quantitation (LOQ) of 10 and 20 µg kg-1, respectively, were obtained. Recoveries (84.3-107.5%) and coefficients of variation (CV<15%) for repeatability and reproducibility conditions were appropriate in intraday and interday laboratory conditions. The concentration of acrylamide was determined in 76 commercial samples of NCS from different Latin American countries and ranged from <20 to 1414 µg kg-1. The highest mean acrylamide amounts were found in samples from Peru, Ecuador and Colombia (636, 446 and 401 µg kg-1, respectively). Considering the form of the product, the highest average levels were found in granulated NCS (517 µg kg-1) when compared to block (294 µg kg-1). Within the Colombian samples, organic NCS, made with natural flocculant, presented a higher acrylamide mean concentration (721 µg kg-1) than conventional samples (363 µg kg-1). Although the Margin of Exposure (MOE) values for neurotoxicity suggested that the estimated intakes in Brazil and Colombia are not a concern, MOEs obtained for neoplastic effects raised attention.
Asunto(s)
Acrilamida/análisis , Exposición Dietética/análisis , Análisis de los Alimentos , Contaminación de Alimentos/análisis , Azúcares/análisis , Brasil , Cromatografía Líquida de Alta Presión , Colombia , Humanos , América Latina , Medición de Riesgo , Espectrometría de Masas en TándemRESUMEN
This study estimates for the first time dietary acrylamide intake in Chile and conducts exposure risk assessments using the margin of exposure (MOE) method. A consumption frequency survey of starchy foods was carried out in the metropolitan region of Santiago, Chile on people from different socioeconomic levels aged between 12 and 65 years old. The acrylamide contents of the most frequently consumed foods were determined by an in-house validated GC-MS technique. The potatoes and bread group contributed ~77% to the dietary acrylamide exposure in Chile, with estimated daily mean exposure of 0.55 µg kgbw-1 day-1 and 0.22 µg kgbw-1 day-1, respectively. Chilean population aged between 12 and 17 years old presented the highest acrylamide intake (mean, 1.27 µg kgbw-1 day-1; 95th percentile, 3.90 µg kgbw-1 day-1). Finally, since the estimated MOEs were lower than 10,000, the dietary acrylamide exposure in the metropolitan region of Santiago, Chile is of public health concern according to the EFSA criteria.
Asunto(s)
Acrilamida/análisis , Exposición Dietética/análisis , Contaminación de Alimentos/análisis , Acrilamida/administración & dosificación , Adolescente , Adulto , Anciano , Niño , Chile , Femenino , Humanos , Masculino , Persona de Mediana Edad , Salud Pública , Medición de Riesgo , Adulto JovenRESUMEN
A risk assessment was performed to estimate the lead (Pb) dietary intake in the Brazilian population. Data on Pb concentration in food were obtained through a systematic review with meta-analyses and from the GEMS/Food database, both involving data through 2018. Food consumption data were obtained from a Brazilian official dietary survey. Pb dietary intakes were estimated by a deterministic approach. Risk characterisation was conducted using the margin of exposure approach (MOE). The total Pb intake in the Brazilian population ranged from 1.53 to 2.01 µg/kg bw/day. Major contributors to Pb dietary exposure were the vegetable and vegetable products and beverage categories. From the results obtained, a risk of increased systolic pressure, nephrotoxicity and decrease of 0.5 point intelligence quotient can be expected, MOE<1, with greater magnitudes of risk observed for men in rural areas and in the Midwest, Southeast and South Brazilian regions. The dietary risk assessment indicates a health concern for the Brazilian population associated with Pb dietary intake.
Asunto(s)
Exposición Dietética/análisis , Análisis de los Alimentos , Contaminación de Alimentos/análisis , Plomo/análisis , Brasil , Humanos , Medición de RiesgoRESUMEN
Selenium (Se) is necessary for several physiological functions in the human body; however, high concentrations of this element in coal mining areas raise the possibility of Se-related health risks. Children are much more vulnerable and at risk to environmental hazards than adults. The largest coal mining area of Brazil is located in the city of Candiota, where previous studies point to significant urinary Se concentrations among children. Food intake is the main Se source. The study aimed to evaluate dietary Se intake, as well as urinary Se excretion and classic symptoms of Se intoxication among children from Candiota and a control city in the same region. A cross-sectional study was carried out, with participation from 242 children between 6 and 12 years old in two cities in Rio Grande do Sul state, Brazil. Socioeconomic variables, dietary intake, and Se toxicity symptoms were evaluated through a structured questionnaire, and urinary Se levels were measured. Children from both cities had normal levels of Se intake and urinary excretion; however, children from Candiota had significantly higher levels of Se in both parameters in relation to the control city, especially for Se urinary excretion. There was low prevalence of Se toxicity symptoms. We conclude that coal mining activities may increase Se intake in children and consequently its urinary excretion.
Asunto(s)
Minas de Carbón , Exposición Dietética/análisis , Selenio/orina , Brasil , Niño , Ciudades , Estudios Transversales , Exposición Dietética/estadística & datos numéricos , Femenino , Humanos , Masculino , Selenio/toxicidadRESUMEN
Heavy metals threaten communities near biodiversity hotspots, as their protein sources come from the environment. This study assessed Hg, Cd, and Se concentrations in fish, as well as the magnitude of exposure and hematological conditions of adult citizens from Puerto Nariño (Colombian Amazon). Among fish samples, greater Hg concentrations were found in higher trophic level species, including Rhaphiodon vulpinus (880 ± 130 ng/g) and Pseudoplatystoma tigrinum (920 ± 87 ng/g). These species presented the highest hazard quotients and lowest Se:Hg molar ratios among those studied, showing their consumption represents a health risk to consumers. Moreover, some samples of Mylossoma duriventre and Prochilodus magdalenae had Cd levels greater than the regulated limit (100 ng/g). The average total Hg (T-Hg) concentrations in human hair and blood were 5.31 µg/g and 13.7 µg/L, respectively. All hair samples exceeded the 1.0 µg/g threshold set by the USEPA, whereas 93% of the volunteers had T-Hg blood levels greater than 5 µg/L, suggesting elevated exposure. The mean Cd level was 3.1 µg/L, with 21% of samples surpassing 5 µg/L, value at which mitigating actions should be taken. Eighty-four percent of participants presented Se deficiencies (<100 µg/L). There was a significant association between fish consumption and T-Hg in hair (ρ = 0.323; p = 0.032) and blood (ρ = 0.381; p = 0.011). In this last matrix, Se correlated with Cd content, whereas lymphocytes were inversely linked to Hg concentrations. The results of this study show that there is extensive exposure to Hg in fish, the consumption of which may promote detrimental impacts on hematology parameters within the community.
Asunto(s)
Monitoreo Biológico/métodos , Cadmio/análisis , Peces/metabolismo , Cabello/química , Mercurio/análisis , Selenio/análisis , Adulto , Animales , Cadmio/metabolismo , Colombia , Exposición Dietética/análisis , Femenino , Contaminación de Alimentos/análisis , Humanos , Masculino , Mercurio/metabolismo , Selenio/metabolismoRESUMEN
To investigate the risks posed by trace and rare earth elements (REEs) in two tropical uranium ore fields, metal concentrations from 50 vegetable samples (corn and soybean) and their corresponding agricultural soils were evaluated in a U mining area and a U-rich coal mining area in Brazil. Samples from both areas had metal concentrations (REE: La to Lu, and trace elements: As, Pb, Cd, Ni, Cu, Cr, Mn, Zn, Ba, U, Sr) that were higher than the guidelines proposed by the Brazilian environmental agency. Soils from the U mining area (Poços de Caldas) generally had higher contents of trace elements than the coal mining area (Figueira), with the exception of Ni and Cr, indicating a higher risk of pollution, which was confirmed by a pollution load index that was greater than unity. For both sites, concentrations of uranium in the soil and plants, its hazard quotients and the soil contamination factor were higher in agricultural fields closer to the mines, indicating that contamination and the consequent risks to human health were distance dependent. REE concentrations averaged 52.8 mg kg-1 in the topsoils and 0.76 mg kg-1 in the grains for Figueira, whereas higher values of 371 mg kg-1 (topsoils) and 0.9 mg kg-1 (grains) were found in Poços de Caldas. Based upon corn and soybean consumption, the estimated intake dose of the REE was lower than the intake dose predicted to be problematic for human health for both sites, indicating limited risk related to the ingestion of REE.
Asunto(s)
Productos Agrícolas/química , Metales de Tierras Raras/análisis , Contaminantes del Suelo/análisis , Uranio/análisis , Agricultura , Brasil , Minas de Carbón , Exposición Dietética/efectos adversos , Exposición Dietética/análisis , Monitoreo del Ambiente , Humanos , Minería , Nivel sin Efectos Adversos Observados , Medición de Riesgo , Suelo/química , Oligoelementos/análisis , Zea mays/químicaRESUMEN
This study aims to establish the relationship between the consumption of certain foods and the presence of organochlorine pesticides in human milk. A total of 167 samples of breastmilk were collected. Sample analysis was carried out by gas chromatography with electron capture detector. Pesticide concentrations (op'DDT, pp'DDT, pp'DDE, Σ-DDT, HCB, ß-HCH) were grouped by consumption level of fish, dairy products, meat, eggs, and seafood and then were compared to each other. The odds ratio (OR) was calculated between the concentration of pesticides (higher and/or lower than median) and the exposure variable (high consumption). The results indicated low median concentrations of HCB, ß - HCH, op'DDT and pp'DDT, whose fluctuations and trends between different frequencies of food consumption were not significant. pp'DDE and Σ - DDT values according to the increase in consumption of fish were significantly increased. Women with high fish consumption have a higher risk of high concentrations of pp'DDE and Σ-DDT (OR: high consumption: 5.6 (1.3-23.6). A protective effect was observed in the consumption of dairy products (ß-HCH and op'DDT), meat (HCB), and seafood (pp'DDT). These results suggest that it is possible that the consumption of fish, dairy products, meat, and seafood influences the presence of organochlorine pesticides in the studied population.
Asunto(s)
Exposición Dietética/análisis , Hidrocarburos Clorados/análisis , Leche Humana/química , Plaguicidas/análisis , Adulto , Productos Lácteos , Diclorodifenil Dicloroetileno , Huevos/análisis , Conducta Alimentaria , Femenino , Productos Pesqueros , Contaminación de Alimentos , Humanos , Carne/análisis , MéxicoRESUMEN
Women are primarily exposed to mercury through the consumption of fish contaminated by gold mining activities. The main systems affected are the central nervous and renal systems, although effects on the reproductive system have also been found. Objective: To explore the relationship between mercury levels in women and their possible sources of contamination. A cross-sectional study was conducted from 2013 to 2015 with women residing in 11 municipalities in La Mojana, Colombia, using non-random sampling. Standardized instruments were used to identify sociodemographic characteristics, mercury use, mining-related activities, water and fish consumption, and other factors. Blood, urine, and hair samples were taken to quantify mercury levels. A logistic regression model was used to identify factors associated with elevated mercury values in the participants' samples. A total of 428 women were included, with an average age of 36.7 ± 16.7 years, 3.3% of whom were pregnant at the time of the survey and 8.4% of whom were exposed occupationally. High levels of mercury were present in 62.8% of the women, in any one of the three samples processed. Those exposed occupationally and environmentally had similar values (p = 0.821). Frequency of fish consumption and source of drinking water were associated with higher levels of mercury (p < 0.05).
Asunto(s)
Exposición Dietética/análisis , Mercurio , Adulto , Animales , Colombia , Estudios Transversales , Agua Potable , Femenino , Peces , Oro , Humanos , Mercurio/análisis , Persona de Mediana Edad , Minería , Embarazo , Adulto JovenRESUMEN
The occurrence of aflatoxin M1 (AFM1) in infant formulae commercialized in the metropolitan area of Monterrey (Nuevo León, Mexico) was determined by using immunoaffinity column clean-up followed by HPLC determination with fluorimetric detection. For this, 55 infant formula powders were classified in two groups, starter (49 samples) and follow-on (6 samples) formulae. Eleven of the evaluated samples (20%) presented values above the permissible limit set by the European Union for infant formulae (25 ng/L), ranging from 40 to 450 ng/L. The estimated daily intake (EDI) for AFM1 was determined employing the average body weight (bw) of the groups of age in the ranges of 0-6 and 6-12 months, and 1-2 years. The results evidenced high intake values, ranging from 1.56 to 14 ng/kg bw/day, depending on the group. Finally, with the EDI value, the carcinogenic risk index was determined, presenting a high risk for all the evaluated groups. Based on these results, it is a necessary extra effort by the regulatory agencies to reduce the AFM1 presence in infant formulae consumed in Mexico.
Asunto(s)
Aflatoxina M1/análisis , Exposición Dietética/análisis , Contaminación de Alimentos/análisis , Fórmulas Infantiles/análisis , Peso Corporal , Preescolar , Monitoreo del Ambiente , Humanos , Lactante , Recién Nacido , México , Neoplasias , Medición de RiesgoRESUMEN
The presence of substances such as hormones and toxic metal in aquatic ecosystem is interesting to the scientific community due to their adverse effects. We quantified 17α-ethynylestradiol (EE2) and toxic metals in the surface waters from Sorocaba and Pirajibu Rivers, in São Paulo State, and we estimated the daily intake for hormone, based on the amount of water consumed. EE2, Cd, Hg, As, Pb, and Mn were seasonally quantified in six different locations along the rivers. EE2 was evaluated by high-performance liquid chromatography. Toxic metals were determined by inductively coupled plasma mass spectrometer. Considering the entire sample year, EE2 concentrations ranged from 4.5 to 48.2 µg L-1. Comparing Sorocaba and Pirajibu rivers, the sample point in the entrance of the Pirajibu River through the city of Itu, São Paulo State, had higher amounts of EE2. Regarding metals, all results are according to the Brazilian and World Health Organization guidelines for drinking-water quality, except for Mn levels, which were higher than the limits in Autumn season in two locations. The estimated daily intake ranged from 13.45 to 40.9 µg/day/person. In conclusion, concentrations of EE2 in the Sorocaba and Pirajibu Rivers were higher than in other countries. The levels were as high as an intake of one pill for each person every day (considering an oral contraceptive has 0.03 mg of ethinylestradiol). Even though concentrations of toxic elements are in accordance with the Brazilian Regulation and World Health Organization, legislation for hormones and drugs needs to advance.
Asunto(s)
Exposición Dietética/análisis , Etinilestradiol/análisis , Metales/análisis , Contaminantes Químicos del Agua/análisis , Brasil , Exposición Dietética/estadística & datos numéricos , Ecosistema , Monitoreo del Ambiente , Intoxicación por Metales Pesados , Mercurio/análisis , Ríos/química , Calidad del AguaRESUMEN
Celiac disease, wheat sensitivity, and allergy represent three different reactions, which may occur in genetically predisposed individuals on the ingestion of wheat and derived products with various manifestations. Improvements in the disease diagnostics and understanding of disease etiology unveiled that these disorders are widespread around the globe affecting about 7% of the population. The only known treatment so far is a life-long gluten-free diet, which is almost impossible to follow because of the contamination of allegedly "gluten-free" products. Accidental contamination of inherently gluten-free products could take place at any level from field to shelf because of the ubiquity of these proteins/grains. Gluten contamination of allegedly "gluten-free" products is a constant threat to celiac patients and a major health concern. Several detection procedures have been proposed to determine the level of contamination in products for celiac patients. The present article aims to review the advantages and disadvantages of different gluten detection methods, with emphasis on the recent technology that allows identification of the immunogenic-gluten peptides without the use of antibodies. The possibility to detect gluten contamination by different approaches with similar or better detection efficiency in different raw and processed foods will guarantee the safety of the foods for celiac patients.