RESUMEN
Xanthan, the main exopolysaccharide (EPS) synthesized by Xanthomonas spp., contributes to bacterial stress tolerance and enhances attachment to plant surfaces by helping in biofilm formation. Therefore, xanthan is essential for successful colonization and growth in planta and has also been proposed to be involved in the promotion of pathogenesis by calcium ion chelation and, hence, in the suppression of the plant defense responses in which this cation acts as a signal. The aim of this work was to study the relationship between xanthan structure and its role as a virulence factor. We analyzed four Xanthomonas campestris pv. campestris mutants that synthesize structural variants of xanthan. We found that the lack of acetyl groups that decorate the internal mannose residues, ketal-pyruvate groups, and external mannose residues affects bacterial adhesion and biofilm architecture. In addition, the mutants that synthesized EPS without pyruvilation or without the external mannose residues did not develop disease symptoms in Arabidopsis thaliana. We also observed that the presence of the external mannose residues and, hence, pyruvilation is required for xanthan to suppress callose deposition as well as to interfere with stomatal defense. In conclusion, pyruvilation of xanthan seems to be essential for Xanthomonas campestris pv. campestris virulence.
Asunto(s)
Arabidopsis/microbiología , Biopelículas/crecimiento & desarrollo , Glucanos/metabolismo , Enfermedades de las Plantas/microbiología , Polisacáridos Bacterianos/química , Xanthomonas campestris/patogenicidad , Interacciones Huésped-Patógeno , Mutación , Hojas de la Planta/microbiología , Estomas de Plantas/microbiología , Polisacáridos Bacterianos/genética , Polisacáridos Bacterianos/metabolismo , Ácido Pirúvico/química , Virulencia , Factores de Virulencia/química , Factores de Virulencia/genética , Factores de Virulencia/metabolismo , Xanthomonas campestris/genética , Xanthomonas campestris/crecimiento & desarrollo , Xanthomonas campestris/fisiologíaRESUMEN
This study investigated the effect of silicon (Si) on the potentiation of rice resistance against leaf scald at the microscopic level. Rice plants ('Primavera') were grown in a nutrient solution containing 0 (-Si) or 2 mM (+Si) Si. The foliar Si concentration of the +Si plants (3.6 dag/kg) increased in comparison with the -Si plants (0.3 dag/kg). An X-ray microanalysis revealed that the leaf tissue of +Si plants infected with Microdochium oryzae had higher peaks and deposition of insoluble Si than that of -Si plants. The high foliar Si concentration for the +Si plants reduced the expansion of leaf scald lesions. Scanning electron microscopy revealed that fungal hyphae and appressorium-like structures of M. oryzae were more abundant in the leaf surface of -Si plants relative to +Si plants. At both histopathological and ultrastructural levels, fungal hyphae grew abundantly into the leaf tissue of -Si plants. By contrast, rice cell walls were rarely degraded and fungal hyphae were often surrounded by amorphous granular material in the leaf tissue of +Si plants. Conidiophores emerged from stomata 36 h after fungal penetration, and conidia were noticed inside the leaf tissue of the -Si plants in great abundance. The collective results of the present study showed a high concentration and deposition of Si and a considerable deposition of phenolic-like compounds in the leaf tissue of +Si plants. These results indicate that the potentiation of the phenylpropanoid pathway in these plants supplied with Si was favorable for the increase in rice resistance to leaf scald.
Asunto(s)
Ascomicetos/fisiología , Oryza/inmunología , Enfermedades de las Plantas/inmunología , Silicio/farmacología , Ascomicetos/patogenicidad , Pared Celular/metabolismo , Resistencia a la Enfermedad , Microanálisis por Sonda Electrónica , Hifa , Microscopía Electrónica de Rastreo , Oryza/efectos de los fármacos , Oryza/microbiología , Oryza/ultraestructura , Enfermedades de las Plantas/microbiología , Hojas de la Planta/inmunología , Hojas de la Planta/microbiología , Hojas de la Planta/ultraestructura , Estomas de Plantas/inmunología , Estomas de Plantas/microbiología , Estomas de Plantas/ultraestructura , Esporas FúngicasRESUMEN
Xanthomonas axonopodis pv. citri (Xac) causes citrus canker, provoking defoliation and premature fruit drop with concomitant economical damage. In plant pathogenic bacteria, lipopolysaccharides are important virulence factors, and they are being increasingly recognized as major pathogen-associated molecular patterns for plants. In general, three domains are recognized in a lipopolysaccharide: the hydrophobic lipid A, the hydrophilic O-antigen polysaccharide, and the core oligosaccharide, connecting lipid A and O-antigen. In this work, we have determined the structure of purified lipopolysaccharides obtained from Xanthomonas axonopodis pv. citri wild type and a mutant of the O-antigen ABC transporter encoded by the wzt gene. High pH anion exchange chromatography and matrix-assisted laser desorption/ionization mass spectrum analysis were performed, enabling determination of the structure not only of the released oligosaccharides and lipid A moieties but also the intact lipopolysaccharides. The results demonstrate that Xac wild type and Xacwzt LPSs are composed mainly of a penta- or tetra-acylated diglucosamine backbone attached to either two pyrophosphorylethanolamine groups or to one pyrophosphorylethanolamine group and one phosphorylethanolamine group. The core region consists of a branched oligosaccharide formed by Kdo2Hex6GalA3Fuc3NAcRha4 and two phosphate groups. As expected, the presence of a rhamnose homo-oligosaccharide as O-antigen was determined only in the Xac wild type lipopolysaccharide. In addition, we have examined how lipopolysaccharides from Xac function in the pathogenesis process. We analyzed the response of the different lipopolysaccharides during the stomata aperture closure cycle, the callose deposition, the expression of defense-related genes, and reactive oxygen species production in citrus leaves, suggesting a functional role of the O-antigen from Xac lipopolysaccharides in the basal response.
Asunto(s)
Citrus sinensis/inmunología , Citrus sinensis/microbiología , Inmunidad Innata , Lipopolisacáridos/química , Lipopolisacáridos/metabolismo , Xanthomonas axonopodis/fisiología , Transportadoras de Casetes de Unión a ATP/química , Transportadoras de Casetes de Unión a ATP/genética , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Secuencia de Carbohidratos , Citrus sinensis/anatomía & histología , Citrus sinensis/genética , Regulación de la Expresión Génica de las Plantas/inmunología , Interacciones Huésped-Patógeno , Inmunidad Innata/genética , Lipopolisacáridos/biosíntesis , Lipopolisacáridos/aislamiento & purificación , Datos de Secuencia Molecular , Peróxidos/metabolismo , Hojas de la Planta/genética , Hojas de la Planta/inmunología , Hojas de la Planta/microbiología , Estomas de Plantas/anatomía & histología , Estomas de Plantas/inmunología , Estomas de Plantas/microbiología , Xanthomonas axonopodis/metabolismoRESUMEN
The phyllosphere, i.e., the aerial parts of the plant, provides one of the most important niches for microbial colonization. This niche supports the survival and, often, proliferation of microbes such as fungi and bacteria with diverse lifestyles including epiphytes, saprophytes, and pathogens. Although most microbes may complete the life cycle on the leaf surface, pathogens must enter the leaf and multiply aggressively in the leaf interior. Natural surface openings, such as stomata, are important entry sites for bacteria. Stomata are known for their vital role in water transpiration and gas exchange between the plant and the environment that is essential for plant growth. Recent studies have shown that stomata can also play an active role in limiting bacterial invasion of both human and plant pathogenic bacteria as part of the plant innate immune system. As counter-defense, plant pathogens such as Pseudomonas syringae pv tomato (Pst) DC3000 use the virulence factor coronatine to suppress stomate-based defense. A novel and crucial early battleground in host-pathogen interaction in the phyllosphere has been discovered with broad implications in the study of bacterial pathogenesis, host immunity, and molecular ecology of bacterial diseases.
Asunto(s)
Aminoácidos/metabolismo , Indenos/metabolismo , Solanum lycopersicum/fisiología , Hojas de la Planta/fisiología , Estomas de Plantas/fisiología , Pseudomonas syringae/patogenicidad , Factores de Virulencia/fisiología , Aminoácidos/genética , Solanum lycopersicum/genética , Solanum lycopersicum/microbiología , Hojas de la Planta/microbiología , Estomas de Plantas/microbiología , Pseudomonas syringae/genética , Factores de Virulencia/genéticaRESUMEN
The phyllosphere, i.e., the aerial parts of the plant, provides one of the most important niches for microbial colonization. This niche supports the survival and, often, proliferation of microbes such as fungi and bacteria with diverse lifestyles including epiphytes, saprophytes, and pathogens. Although most microbes may complete the life cycle on the leaf surface, pathogens must enter the leaf and multiply aggressively in the leaf interior. Natural surface openings, such as stomata, are important entry sites for bacteria. Stomata are known for their vital role in water transpiration and gas exchange between the plant and the environment that is essential for plant growth. Recent studies have shown that stomata can also play an active role in limiting bacterial invasion of both human and plant pathogenic bacteria as part of the plant innate immune system. As counter-defense, plant pathogens such as Pseudomonas syringae pv tomato (Pst) DC3000 use the virulence factor coronatine to suppress stomate-based defense. A novel and crucial early battleground in host-pathogen interaction in the phyllosphere has been discovered with broad implications in the study of bacterial pathogenesis, host immunity, and molecular ecology of bacterial diseases.
Asunto(s)
Aminoácidos/metabolismo , Indenos/metabolismo , Hojas de la Planta/fisiología , Estomas de Plantas/fisiología , Pseudomonas syringae/patogenicidad , Solanum lycopersicum/fisiología , Factores de Virulencia/fisiología , Aminoácidos/genética , Solanum lycopersicum/genética , Solanum lycopersicum/microbiología , Hojas de la Planta/microbiología , Estomas de Plantas/microbiología , Pseudomonas syringae/genética , Factores de Virulencia/genéticaRESUMEN
Bacteria and fungi are capable of triggering stomatal closure through pathogen-associated molecular patterns (PAMPs), which prevents penetration through these pores. Therefore, the stomata can be considered part of the plant innate immune response. Some pathogens have evolved mechanisms to evade stomatal defense. The bacterial pathogen Xanthomonas campestris pv. campestris (Xcc), which infects plants of the Brassicaceae family mainly through hydathodes, has also been reported to infect plants through stomata. A recent report shows that penetration of Xcc in Arabidopsis leaves through stomata depends on a secreted small molecule whose synthesis is under control of the rpf/diffusible signal factor (DSF) cell-to-cell signaling system, which also controls genes involved in biofilm formation and pathogenesis. The same reports shows that Arabidopsis ROS- and PAMP-activated MAP kinase 3 (MPK3) is essential for stomatal innate response. Other recent and past findings about modulation of stomatal behaviour by pathogens are also discussed. In all, these findings support the idea that PAMP-triggered stomatal closure might be a more effective and widespread barrier against phytopathogens than previously thought, which has in turn led to the evolution in pathogens of several mechanisms to evade stomatal defense.