RESUMEN
CONTEXT: Mutations in the electron donor enzyme P450 oxidoreductase (POR) result in congenital adrenal hyperplasia with apparent combined 17α-hydroxylase/17,20 lyase and 21-hydroxylase deficiencies, also termed P450 oxidoreductase deficiency (PORD). Major clinical features present in PORD are disordered sex development in affected individuals of both sexes, glucocorticoid deficiency, and multiple skeletal malformations. OBJECTIVE: The objective of the study was to establish a noninvasive approach to prenatal diagnosis of PORD including assessment of malformation severity to facilitate optimized prenatal diagnosis and timely treatment. DESIGN: We analyzed 20 pregnancies with children homozygous or compound heterozygous for disease-causing POR mutations and 1 pregnancy with a child carrying a heterozygous POR mutation by recording clinical and biochemical presentations and fetal ultrasound findings. In 4 of the pregnancies (3 homozygous and 1 heterozygous for disease-causing POR mutations), prenatal analysis of steroid metabolite excretion in maternal urine was carried out by gas chromatography/mass spectrometry during gestational weeks 11-23. RESULTS: Pregnancy complications in our cohort included maternal virilization (6 of 20) with onset in the second trimester. Seven pregnant women presented with low unconjugated estriol at prenatal screening (triple or quadruple antenatal screening test). Overt dysmorphic features were noted in 19 of the 20 babies at birth but observed in only 5 by prenatal ultrasound. These 5 had the most severe malformation phenotypes and poor outcome, whereas the other babies showed normal development. Steroid profiling of maternal urine revealed significantly increased steroids of fetal origin, namely the pregnenolone metabolite epiallopregnanediol and the androgen metabolite androsterone, with concomitant low values for estriol. Diagnostic steroid ratios conclusively indicated PORD as early as gestational week 12. In the heterozygous pregnancy, steroid ratios were only slightly elevated and estriol excretion was normal. CONCLUSION: Prenatal diagnosis in PORD is readily established via urinary steroid metabolite analysis of maternal urine. Visible malformations at prenatal ultrasound predict a severe malformation phenotype.
Asunto(s)
Anomalías Múltiples/diagnóstico por imagen , Hiperplasia Suprarrenal Congénita , Tamizaje Masivo/métodos , Diagnóstico Prenatal/métodos , Esteroide 17-alfa-Hidroxilasa/orina , Esteroide 21-Hidroxilasa/orina , Anomalías Múltiples/genética , Hiperplasia Suprarrenal Congénita/diagnóstico , Hiperplasia Suprarrenal Congénita/genética , Hiperplasia Suprarrenal Congénita/orina , Androsterona/orina , Estriol/orina , Femenino , Heterocigoto , Homocigoto , Humanos , Masculino , Fenotipo , Valor Predictivo de las Pruebas , Embarazo , Segundo Trimestre del Embarazo/genética , Pregnanodiol/orina , Radiografía , Esteroide 17-alfa-Hidroxilasa/genética , Esteroide 21-Hidroxilasa/genética , Ultrasonografía Prenatal , Virilismo/diagnóstico , Virilismo/genéticaRESUMEN
Several factors influencing the carbon isotope ratios (CIR) of endogenous urinary steroids have been identified in recent years. One of these should be the metabolism of steroids inside the body involving numerous different enzymes. A detailed look at this metabolism taking into account differences found between steroids excreted as glucuronides or as sulphates and hydrogen isotope ratios of different steroids pointed out possibility of unequal CIR at the main production sites inside the male body - the testes and the adrenal glands. By administration of ß-HCG it is possible to strongly stimulate the steroid production within the testes without influencing the production at the adrenal glands. Therefore, this treatment should result in changed CIR of urinary androgens in contrast to the undisturbed pre-treatment values. Four male volunteers received three injections of ß-HCG over a time course of 5 days and collected their urine samples at defined intervals after the last administration. Those samples showing the largest response in contrast to the pre-administration urines were identified by steroid profile measurements and subsequent analysed by GC/C/IRMS. CIR of androsterone, etiocholanolone, testosterone, 5α- and 5ß-androstanediol and pregnanediol were compared. While pregnanediol was not influenced, most of the investigated androgens showed depleted values after treatment. The majority of differences were found to be statistically significant and nearly all showed the expected trend towards more depleted δ(13)C-values. These results support the hypothesis of different CIR at different production sites inside the human body. The impact of these findings on doping control analysis will be discussed.
Asunto(s)
Gonadotropina Coriónica Humana de Subunidad beta/administración & dosificación , Gonadotropina Coriónica Humana de Subunidad beta/farmacología , Esteroides/química , Esteroides/orina , Adulto , Isótopos de Carbono/análisis , Colesterol/orina , Doping en los Deportes/prevención & control , Humanos , Masculino , Esteroide 17-alfa-Hidroxilasa/orina , Sulfatos/química , Urinálisis , Adulto JovenRESUMEN
OBJECTIVES: (1) Characterize serum (S) and urinary (U) steroid metabolites in complete CYP17 deficiency (cCYP17D); (2) analyze the relative 17α-hydroxylase (17OH) and 17,20-lyase (17,20L) activities in vivo; and (3) comparedata from the two most prevalent mutations in Brazil. SUBJECTS AND METHODS: 20 genotyped cCYP17D patients from a previously reported cohort were homozygous for W406R or R362C; 11 controls were CYP17 wild types (WT). WT and cCYP17D patients had S and U samples drawn to measure: cortisol (F), corticosterone (B), deoxycorticosterone (DOC), 18OH-B, 18OH-DOC, and 17OHP; and tetrahydro (TH)-B, THA, THDOC, THF+5α-THF, TH-cortisone, androsterone, etiocholanolone, 5-pregnenediol, 17OH-pregnenolone and pregnanetriol. RESULTS: Compared to WT, cCYP17D patients had marked elevations of B, DOC, 18OH-B and 18OH-DOC, whereas 17OHP, F and adrenal androgens (AA) were reduced; U steroids parallel S findings. Metabolite ratios revealed that both 17OH and 17,20L activities were impaired in cCYP17D. There were nodifferences between W406R andR362C mutations. CONCLUSIONS: cCYP17D patients show parallel overproduction/overexcretion of 17-deoxysteroids, and marked reduction of F and AA. In addition to 17OH, 17,20-L activity was also impaired in cCYP17D. W406 and R362C mutations disclose similar Sand U patterns.
Asunto(s)
Hiperplasia Suprarrenal Congénita/orina , Esteroide 17-alfa-Hidroxilasa/orina , Adolescente , Hiperplasia Suprarrenal Congénita/genética , Adulto , Andrógenos/orina , Estudios de Casos y Controles , Femenino , Humanos , Hidrocortisona/orina , Masculino , Persona de Mediana Edad , Mutación/genética , Esteroide 17-alfa-Hidroxilasa/genética , Adulto JovenRESUMEN
For patients with cytochrome P450 oxidoreductase deficiency (PORD), steroid replacement is recommended at times of stress. However, it is unknown how hormones respond to actual physical stress in these patients. We report a female infant with PORD accompanied by the Antley-Bixler syndrome phenotype. Her urinary steroid profile revealed defective CYP17A1 and CYP21A2 activities, and an adrenocorticotropin (ACTH) stimulation test showed potential adrenal insufficiency. Hormonal responses to actual physical stress were as follows: Vigorous crying during blood sampling rarely affected the serum cortisol level. Acute viral gastroenteritis led to marked increases in blood ACTH and 17alpha-hydroxyprogesterone levels in proportion to the severity of the illness. The serum cortisol level also responded to this stress, but the response might have been blunted. Regarding peri-operative steroid replacement, intravenous hydrocortisone administration even at a dose of 6 mg/kg, which is lower than that recommended for congenital adrenal hyperplasia in Japan, proved to be excessive.
Asunto(s)
Insuficiencia Suprarrenal/metabolismo , Fenotipo del Síndrome de Antley-Bixler/metabolismo , NADPH-Ferrihemoproteína Reductasa/deficiencia , Estrés Fisiológico/fisiología , Insuficiencia Suprarrenal/complicaciones , Fenotipo del Síndrome de Antley-Bixler/complicaciones , Preescolar , Femenino , Humanos , Hidrocortisona/sangre , Lactante , Recién Nacido , NADPH-Ferrihemoproteína Reductasa/metabolismo , Oxidorreductasas/deficiencia , Oxidorreductasas/metabolismo , Esteroide 17-alfa-Hidroxilasa/orina , Esteroide 21-Hidroxilasa/orinaRESUMEN
In the 1930s, Stein and Leventhal added amenorrhoea to anovulatory dysfunctional uterine bleeding among the known clinical manifestations of the polycystic ovary syndrome (PCOS). Whatever the menstrual pattern, infrequent or absent ovulation with symmetrical enlargement of the ovaries is now a familiar abnormality in women of reproductive age. Diagnosis of PCOS has developed from just the clinically obvious to an appreciation, through ultrasound imaging of the ovaries and endocrine testing, of its subtler forms. Today's clinicians will identify PCOS on the ultrasound image of many small follicles apparent in the periphery of both ovaries, on raised serum unbound testosterone assays, on exaggeration of serum LH levels with the start of pulsatile GnRH therapy, and on follicular overresponsiveness to injections of FSH. Once among the most treatable causes of infertility, ovulation-induction for PCOS remained unsophisticated while microsurgery and assisted conception dissolved frontiers for other causes of infertility. Whereas we now have the benefit of high technology embryo cryostorage to cope with embarrassingly high yields of PCOS oocytes, we still need to explain why, the bigger the ovaries, the more likely (we have long known it to be) that PCOS can be cured simply by reducing ovarian mass. Some cases of PCOS are hereditary and most seem constitutionally determined. PCOS is so common that the questions must be asked, Are we appreciating an extreme of normal? Could the milder forms of PCOS have--or could PCOS have had--evolutionary usefulness?
Asunto(s)
Hormona Folículo Estimulante/uso terapéutico , Hormona Liberadora de Gonadotropina/uso terapéutico , Gonadotropinas/uso terapéutico , Hormona del Crecimiento/uso terapéutico , Ovario/fisiopatología , Ovulación/fisiología , Síndrome del Ovario Poliquístico/tratamiento farmacológico , Síndrome del Ovario Poliquístico/fisiopatología , Clomifeno/farmacología , Clomifeno/uso terapéutico , Femenino , Hormona Liberadora de Gonadotropina/administración & dosificación , Humanos , Inyecciones Intravenosas , Hormona Luteinizante/sangre , Ovario/cirugía , Ovulación/efectos de los fármacos , Síndrome del Ovario Poliquístico/etiología , Somatostatina/uso terapéutico , Esteroide 17-alfa-Hidroxilasa/orinaRESUMEN
Twenty four-hour urine samples have been collected 6 monthly for 18 months from 127 normal boys aged between 7.5 and 17 years. Urinary steroid metabolites have been measured by gas-liquid chromatography and ratios of steroid metabolites used to draw conclusions about likely enzymic activities at each step in the adrenal biosynthetic pathway. DHA excretion rose throughout the study, especially between 7.5 and 11 years. Metabolites of cortisol and corticosterone did not increase when related to surface area. During adrenarche there were changes consistent with a decrease in 3 beta-hydroxysteroid dehydrogenase activity, a slight rise in 17 alpha hydroxylase activity, a marked rise in 17,20 lyase activity, and a fall in 11 beta hydroxylase activity. These data are compatible with the hypothesis that a small reduction in the ability to synthesize cortisol requires a marginal increase in the secretion of ACTH and/or other CRF-induced peptides to maintain cortisol production rate. ACTH itself or a dexamethasone-suppressible CRF-induced peptide would in such a case control the growth of the zona reticularis at adrenarche, which thus appears to be primarily an adrenal event.