RESUMEN
Liquid biopsy for circulating tumour cell (CTC) detection is generally unexplored in veterinary medicine. Dogs with highly aggressive and heterogeneous tumours, such as oral malignant melanoma (OMM), could benefit from studies involving size-based isolation methods for CTCs, as they do not depend on specific antibodies. This pilot study aimed to detect CTCs from canine OMM using Isolation by Size of Epithelial Tumor Cells (ISET), a microfiltration methodology, followed by immunocytochemistry (ICC) with Melan-A, PNL2, and S100 antibodies. Ten canine patients diagnosed by histopathology and confirmed as OMM by immunohistochemistry were enrolled, their prognostic data was assessed, and blood samples were collected for CTC analysis. Results have shown the detection of intact cells in 9/10 patients. ICC has shown 3/9 Melan-A-positive, 3/9 PNL2-positive, and 8/9 S100-positive patients, confirming the importance of opting for a multimarker assay. A significant number of negative-stained CTCs were found, suggesting their high heterogeneity in circulation. Microemboli stained with either PNL2 or S100 were found in a patient with a high isolated cell count and advanced clinical stage. Preliminary statistical analysis shows a significant difference in CTC count between patients with and without lymph node metastasis (p < .05), which may correlate with tumour metastatic potential. However, we recommend further studies with more extensive sampling to confirm this result. This pilot study is the first report of intact CTC detection in canine OMM and the first application of ISET in veterinary medicine, opening new possibilities for liquid biopsy studies in canine OMM and other tumours.
Asunto(s)
Enfermedades de los Perros , Melanoma , Neoplasias de la Boca , Células Neoplásicas Circulantes , Perros , Animales , Enfermedades de los Perros/patología , Enfermedades de los Perros/sangre , Enfermedades de los Perros/diagnóstico , Proyectos Piloto , Células Neoplásicas Circulantes/patología , Neoplasias de la Boca/veterinaria , Neoplasias de la Boca/patología , Neoplasias de la Boca/diagnóstico , Neoplasias de la Boca/sangre , Melanoma/veterinaria , Melanoma/patología , Melanoma/sangre , Melanoma/diagnóstico , Masculino , Femenino , Inmunohistoquímica/veterinaria , Biomarcadores de Tumor/sangreRESUMEN
The objective of this study was to investigate the presence and genetic attributes of Borrelia spp. in cats and dogs from the West Azerbaijan Province, located in the northwest of Iran. A total of 250 blood samples from cats and 300 blood samples from dogs were collected, and information regarding their age, sex, breed, ownership status, sampling time and region was recorded. The identification of positive samples was accomplished through nested-PCR and sequencing, with subsequent analysis of the gene sequences conducted using BioEdit software. The gene sequences for Borrelia spp. in this study showed 100% similarity to reference sequences in the GenBank® database. Phylogenetic trees were built using MEGA11. The outcomes indicated that among 250 blood samples from cats, 48 (19.2%) tested positive for Borrelia spp. gene, with a CI from 14.8 to 24.53% for cats. Similarly, out of 300 blood samples from dogs, 45 (15%) tested positive for the Borrelia spp. gene, with a CI from 11.4 to 19.48% for dogs.
Asunto(s)
Borrelia , Enfermedades de los Gatos , Enfermedades de los Perros , Filogenia , Reacción en Cadena de la Polimerasa , Animales , Perros , Irán , Gatos , Enfermedades de los Perros/microbiología , Enfermedades de los Perros/sangre , Enfermedades de los Gatos/microbiología , Enfermedades de los Gatos/sangre , Borrelia/genética , Borrelia/clasificación , Borrelia/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Femenino , Masculino , Infecciones por Borrelia/veterinaria , Infecciones por Borrelia/microbiología , ADN Bacteriano/genéticaRESUMEN
BACKGROUND: Visceral leishmaniasis (VL) is a zoonotic disease, with dogs being the main reservoir of the Leishmania infantum parasite. OBJECTIVE: To develop a new flow cytometry test to diagnosis canine VL (CVL) diagnosis. METHODS: The current study addresses a new flow cytometry test using beads coupled to the multiepitope antigen rMELEISH. RESULTS: In the study set of samples a sensitivity (87.1%) and specificity (89.9%) was observed. Considering the dogs' clinical status, 20/20 (100.0%) of the symptomatic sera tested positive, while 19/22 (86.4%) of the oligosymptomatic and 16/20 (80.0%) of asymptomatic were positive. In the non-infected control, all samples (0/30) tested as negative. In the cross-reaction control, the test was more efficient in dogs infected with L. braziliensis (2/10) and Trypanosoma cruzi (0/10), than those with Babesia canis (4/10) and Ehrlichia canis (4/10). Dogs immunized with different vaccines (Leishmune, Leish-Tec®, or LBSap) did not present serological reactivity. CONCLUSION: The flow cytometry serology through coupling the antigen rMELEISH in functional beads showed high accuracy in diagnosing CVL.
Asunto(s)
Antígenos de Protozoos , Enfermedades de los Perros , Citometría de Flujo , Leishmaniasis Visceral , Leishmaniasis Visceral/diagnóstico , Leishmaniasis Visceral/veterinaria , Leishmaniasis Visceral/inmunología , Leishmaniasis Visceral/sangre , Animales , Perros , Citometría de Flujo/métodos , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/inmunología , Enfermedades de los Perros/parasitología , Enfermedades de los Perros/sangre , Antígenos de Protozoos/inmunología , Sensibilidad y Especificidad , Epítopos/inmunología , Leishmania infantum/inmunología , Anticuerpos Antiprotozoarios/sangre , Anticuerpos Antiprotozoarios/inmunología , Reacciones Cruzadas/inmunología , Pruebas Serológicas/métodosRESUMEN
In canine leishmaniosis caused by the protozoan Leishmania infantum, little is known about how co-infections with or co-seropositivities for other pathogens can influence aggravation of this disease. Therefore, the objectives of this study were to evaluate the frequency of co-infections with or co-seropositivities for certain pathogens in dogs seropositive for L. infantum and their relationship with clinical signs, histological changes and L. infantum load. Sixty-six L. infantum-seropositive dogs were submitted to clinical examination, collection of blood and bone marrow, culling, and necropsy. Antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato, Ehrlichia spp. and Toxoplasma gondii and Dirofilaria immitis antigens were investigated in serum. Samples from different tissues were submitted to histopathology and immunohistochemistry for the detection of Leishmania spp. and T. gondii. Quantitative real-time PCR was used to assess the L. infantum load in spleen samples. For detection of Coxiella burnetii, conventional PCR and nested PCR were performed using bone marrow samples. All 66 dogs tested positive for L. infantum by qPCR and/or culture. Fifty dogs (76%) were co-seropositive for at least one pathogen: T. gondii (59%), Ehrlichia spp., (41%), and Anaplasma spp. (18%). Clinical signs were observed in 15 (94%) dogs monoinfected with L. infantum and in 45 (90%) dogs co-seropositive for certain pathogens. The L. infantum load in spleen and skin did not differ significantly between monoinfected and co-seropositive dogs. The number of inflammatory cells was higher in the spleen, lung and mammary gland of co-seropositive dogs and in the mitral valve of monoinfected dogs. These results suggest that dogs infected with L. infantum and co-seropositive for certain pathogens are common in the region studied. However, co-seropositivities for certain pathogens did not aggravate clinical signs or L. infantum load, although they were associated with a more intense inflammatory reaction in some organs.
Asunto(s)
Coinfección/sangre , Coinfección/veterinaria , Enfermedades de los Perros/sangre , Ehrlichia canis/inmunología , Ehrlichiosis/sangre , Ehrlichiosis/veterinaria , Leishmania infantum/inmunología , Leishmaniasis Visceral/sangre , Leishmaniasis Visceral/veterinaria , Carga de Parásitos , Toxoplasma/inmunología , Toxoplasmosis Animal/sangre , Animales , Anticuerpos Antiprotozoarios/sangre , Coinfección/parasitología , Coinfección/patología , Enfermedades de los Perros/parasitología , Enfermedades de los Perros/patología , Perros , Ehrlichiosis/parasitología , Ehrlichiosis/patología , Femenino , Inmunohistoquímica/métodos , Leishmaniasis Visceral/parasitología , Leishmaniasis Visceral/patología , Leucocitos/inmunología , Masculino , Células Mieloides/inmunología , Toxoplasmosis Animal/parasitología , Toxoplasmosis Animal/patologíaRESUMEN
Visceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR-432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the "MAPK signaling pathway", one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development.
Asunto(s)
Enfermedades de los Perros/parasitología , Leishmania infantum , Leishmaniasis Visceral/veterinaria , Leucocitos Mononucleares/metabolismo , MicroARNs/metabolismo , Animales , Células Cultivadas , Enfermedades de los Perros/sangre , Enfermedades de los Perros/genética , Enfermedades de los Perros/inmunología , Perros , Regulación hacia Abajo , Leishmaniasis Visceral/sangre , Leishmaniasis Visceral/genética , Leishmaniasis Visceral/inmunología , Sistema de Señalización de MAP QuinasasRESUMEN
A leishmaniose canina (CanL) é uma zoonose causada pelo protozoário da espécie Leishmania infantum. O baço e os linfonodos sofrem alterações morfológicas durante o CanL. Esta pesquisa teve como objetivo realizar um estudo anatomopatológico e imuno-histoquímico desses órgãos em cães reativos para leishmaniose aos testes de Imunoensaio Cromatográfico Dual Path Platform (DPP®) e Ensaio de Imunoabsorção Enzimática (ELISA). Vinte e sete cães foram avaliados ao exame anatomopatológico, com 92,6% exibindo alterações à avaliação macroscópica, especialmente esplenomegalia e linfadenomegalia. Todos os cães apresentaram alterações no baço não relacionadas à carga parasitária, sendo a esplenite granulomatosa a alteração mais grave. Hiperplasia cortical e paracortical difusa e hiperplasia e hipertrofia dos cordões medulares foram observadas nos linfonodos. Formas amastigotas de Leishmania spp. foram encontradas no baço e linfonodo às avaliações histopatológica e imuno-histoquímica, com boa concordância entre os métodos (k = 0,55, p = 0,00124), mas não foi observada diferença na intensidade parasitária entre esses órgãos à imuno-histoquímica (p = 0,23). Conclui-se que baço e linfonodo de cães reativos para leishmaniose aos testes DPP® e ELISA apresentam alterações histomorfológicas decorrentes da doença, independente da carga parasitária, assim como esses órgãos apresentam carga parasitária semelhante ao método imuno-histoquímico.
Canine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishmaniasis in the Dual-path Platform chromatographic immunoassay (DPP®) and Enzyme Immunoabsorption Assay (ELISA). Twenty-seven dogs were evaluated for anatomopathological examination with 92.6% showing changes at gross evaluation, specially splenomegaly and lymphadenomegaly. All dogs showed changes in the spleen unrelated to the parasitic load, with granulomatous splenitis being the most severe change. Diffuse cortical and paracortical hyperplasia, and hyperplasia and hypertrophy of the medullary cords were observed in the lymph node. Amastigote forms of Leishmania spp. were found in the spleen and lymph node at histopathological and immunohistochemical evaluations, with good agreement between these evaluations (k = 0.55, p = 0.00124), but no difference was observed in the parasitic intensity of these organs at immunohistochemistry (p = 0.23). It was concluded that spleen and lymph node from dogs reactive to leishmaniasis on the DPP® and ELISA tests show histomorphological changes resulting from the disease, independent to the parasitic load, as well as these organs show similar parasitic load at immunohistochemical test.
Asunto(s)
Animales , Perros , Bazo/anatomía & histología , Bazo/anomalías , Enfermedades Desatendidas/veterinaria , Enfermedades de los Perros/sangre , Leishmania/patogenicidad , Leishmaniasis/veterinaria , Ganglios Linfáticos/anatomía & histología , Ganglios Linfáticos/anomalías , Ensayo de Inmunoadsorción Enzimática/veterinariaRESUMEN
Canine rheumatoid arthritis is a chronic inflammatory disease that affects the joints of dogs. The inflammation can cause damage to cartilage and bones and early diagnosis is the main factor determining treatment success. This report describes an unusual case of rheumatoid arthritis in a dog, highlighting the relevance of radiographic examination in association with histopathology in the definitive diagnosis. An 8 year old, male German Spitz dog, weighing 5kg was admitted with a history of cruciate ligament rupture and grade IV patella dislocation. Previously, surgery had been performed to correct the cranial cruciate ligament rupture, without success. On physical examination, the dog showed a palmigrade stance, severe pelvic limb lameness, and pain on palpation of all joints. Blood count and biochemical analysis were within normal ranges. Orthogonal radiographs of the joints of the pelvic and thoracic limbs were performed. The lesions were bilaterally symmetrical. Samples were taken from the patellofemoral joints for cytology, microbiology, and histopathologic analysis. Rheumatoid factor antibody assay was negative. In conclusion, the combination of more than one clinical sign and diagnostic tests, such as radiographs suggestive of rheumatoid arthritis, histopathologic analysis of the joints, and rheumatoid factor testing is required to reach a definitive diagnosis of rheumatoid arthritis.
A artrite reumatóide canina é por definição uma doença crônica inflamatória que acomete as articulações de cães. Como resultado, a artrite reumatoide pode causar lesões na cartilagem e nos ossos e o diagnóstico precoce é o principal fator para o sucesso do tratamento ideal. Este relato de caso objetivou descrever um caso incomum de artrite reumatóide em um cão, destacando a relevância do exame radiográfico associado à histopatologia no diagnóstico definitivo. Foi atendido um cão da raça Spitz Alemão, 8 anos, com 5kg de peso. Anteriormente, foi realizada procedimento cirúrgico para correção da ruptura bilateral de ligamento cruzado e luxação de patela grau IV, porém sem sucesso. Ao exame físico, o cão apresentava posição palmígrada, intensa claudicação dos membros pélvicos e sensibilidade dolorosa de todas as articulações à palpação. As análises de hemograma e bioquímica sérica básica estavam dentro dos limites normais. Foram realizadas radiografias ortogonais das articulações dos membros pélvicos e torácicos. A distribuição das lesões foi simétrica e em ambos os lados. Considerando esses achados, foram obtidas amostras biológicas das articulações para análise citológica, microbiológica e análise histopatológica. O anticorpo fator reumatóide também foi realizado e o resultado foi negativo. Diante do exposto, é necessária a associação de mais de um sinal clínico e diferentes exames, como por exemplo, radiografias sugestivas de artrite reumatóide, análise histopatológica das articulações e anticorpo fator reumatóide para alcançar o diagnóstico definitivo de artrite reumatóide.
Asunto(s)
Masculino , Animales , Perros , Artritis Reumatoide/diagnóstico por imagen , Artritis Reumatoide/fisiopatología , Artritis Reumatoide/veterinaria , Enfermedades de los Perros/diagnóstico por imagen , Enfermedades de los Perros/sangreRESUMEN
A anemia hemolítica imunomediada (AHIM) é uma reação de hipersensibilidade tipo II onde ocorre o aumento da destruição das hemácias. Ela pode ser dividida em primaria, caracterizada por não ter uma causa subjacente, e em secundária, pode ser causada por agentes infecciosos, como a micoplasmose, neoplasias, medicamentos e transfusões. O objetivo desse trabalho foi realizar uma análise clínica e laboratorial de um cão com anemia hemolítica imunomediada decorrente da Mycoplasma spp. Uma cadela da raça Pit Bull, com 05 meses de idade, foi levada ao hospital apresentando vômitos, diarreia e anorexia. No exame físico, foi possível observar a presença de carrapatos e mucosas ictéricas. Nos exames hematológicos foi possível evidenciaras alterações morfológicas de hemácias que caracterizavam anemia hemolítica imunomediada e a presença do parasita Mycoplasma spp. Nas análises bioquímicas se observou níveis séricos aumentados de creatinina e ureia. Pode-se concluir que, a observação de Mycoplasma spp. em esfregaços sanguíneos associada às alterações morfológicas eritrocitárias são de relevante importância para o diagnóstico de AHIM, auxiliando assim na instituição da melhor conduta terapêutica, contribuindo para o prognóstico do paciente.
Immune-mediated hemolytic anemia (AHIM) is a type II hypersensitivity reaction, where red blood cell destruction occurs or increases. It can be divided into primary, characterized by not having an underlying cause, and secondary, which can be caused by infectious agents, such as mycoplasmosis, neoplasms, drugs and transfusions. The objective of this work was to perform a clinical and laboratory analysis of a dog with immune-mediated hemolytic anemia due to Mycoplasma spp. A five-months-old female Pit Bull dog was taken to the hospital presenting vomiting, diarrhea and anorexia. On physical examination, it was possible to observe the presence of ticks and icteric mucous membranes. In hematological exams, it was possible to show morphological changes in red blood cells that characterize immune-mediated hemolytic anemia and the presence of the parasite Mycoplasma spp. In the biochemical analyzes it was observed increased serum levels of creatinine and urea. It can be concluded that the observation of Mycoplasma spp. in blood vessels associated with erythrocyte morphological changes are of relevant importance for the diagnosis of AHIM, thus assisting in the practice of a better method of therapy, contributing to the patient's prognosis.
Asunto(s)
Femenino , Animales , Perros , Anemia Hemolítica/diagnóstico , Anemia Hemolítica/parasitología , Anemia Hemolítica/sangre , Anemia Hemolítica/veterinaria , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/sangre , Infecciones por Mycoplasma/inmunología , Infecciones por Mycoplasma/tratamiento farmacológico , Infecciones por Mycoplasma/veterinariaRESUMEN
Canine rheumatoid arthritis is a chronic inflammatory disease that affects the joints of dogs. The inflammation can cause damage to cartilage and bones and early diagnosis is the main factor determining treatment success. This report describes an unusual case of rheumatoid arthritis in a dog, highlighting the relevance of radiographic examination in association with histopathology in the definitive diagnosis. An 8 year old, male German Spitz dog, weighing 5kg was admitted with a history of cruciate ligament rupture and grade IV patella dislocation. Previously, surgery had been performed to correct the cranial cruciate ligament rupture, without success. On physical examination, the dog showed a palmigrade stance, severe pelvic limb lameness, and pain on palpation of all joints. Blood count and biochemical analysis were within normal ranges. Orthogonal radiographs of the joints of the pelvic and thoracic limbs were performed. The lesions were bilaterally symmetrical. Samples were taken from the patellofemoral joints for cytology, microbiology, and histopathologic analysis. Rheumatoid factor antibody assay was negative. In conclusion, the combination of more than one clinical sign and diagnostic tests, such as radiographs suggestive of rheumatoid arthritis, histopathologic analysis of the joints, and rheumatoid factor testing is required to reach a definitive diagnosis of rheumatoid arthritis.(AU)
A artrite reumatóide canina é por definição uma doença crônica inflamatória que acomete as articulações de cães. Como resultado, a artrite reumatoide pode causar lesões na cartilagem e nos ossos e o diagnóstico precoce é o principal fator para o sucesso do tratamento ideal. Este relato de caso objetivou descrever um caso incomum de artrite reumatóide em um cão, destacando a relevância do exame radiográfico associado à histopatologia no diagnóstico definitivo. Foi atendido um cão da raça Spitz Alemão, 8 anos, com 5kg de peso. Anteriormente, foi realizada procedimento cirúrgico para correção da ruptura bilateral de ligamento cruzado e luxação de patela grau IV, porém sem sucesso. Ao exame físico, o cão apresentava posição palmígrada, intensa claudicação dos membros pélvicos e sensibilidade dolorosa de todas as articulações à palpação. As análises de hemograma e bioquímica sérica básica estavam dentro dos limites normais. Foram realizadas radiografias ortogonais das articulações dos membros pélvicos e torácicos. A distribuição das lesões foi simétrica e em ambos os lados. Considerando esses achados, foram obtidas amostras biológicas das articulações para análise citológica, microbiológica e análise histopatológica. O anticorpo fator reumatóide também foi realizado e o resultado foi negativo. Diante do exposto, é necessária a associação de mais de um sinal clínico e diferentes exames, como por exemplo, radiografias sugestivas de artrite reumatóide, análise histopatológica das articulações e anticorpo fator reumatóide para alcançar o diagnóstico definitivo de artrite reumatóide.(AU)
Asunto(s)
Animales , Masculino , Perros , Enfermedades de los Perros/sangre , Enfermedades de los Perros/diagnóstico por imagen , Artritis Reumatoide/diagnóstico por imagen , Artritis Reumatoide/fisiopatología , Artritis Reumatoide/veterinariaRESUMEN
A anemia hemolítica imunomediada (AHIM) é uma reação de hipersensibilidade tipo II onde ocorre o aumento da destruição das hemácias. Ela pode ser dividida em primaria, caracterizada por não ter uma causa subjacente, e em secundária, pode ser causada por agentes infecciosos, como a micoplasmose, neoplasias, medicamentos e transfusões. O objetivo desse trabalho foi realizar uma análise clínica e laboratorial de um cão com anemia hemolítica imunomediada decorrente da Mycoplasma spp. Uma cadela da raça Pit Bull, com 05 meses de idade, foi levada ao hospital apresentando vômitos, diarreia e anorexia. No exame físico, foi possível observar a presença de carrapatos e mucosas ictéricas. Nos exames hematológicos foi possível evidenciaras alterações morfológicas de hemácias que caracterizavam anemia hemolítica imunomediada e a presença do parasita Mycoplasma spp. Nas análises bioquímicas se observou níveis séricos aumentados de creatinina e ureia. Pode-se concluir que, a observação de Mycoplasma spp. em esfregaços sanguíneos associada às alterações morfológicas eritrocitárias são de relevante importância para o diagnóstico de AHIM, auxiliando assim na instituição da melhor conduta terapêutica, contribuindo para o prognóstico do paciente.(AU)
Immune-mediated hemolytic anemia (AHIM) is a type II hypersensitivity reaction, where red blood cell destruction occurs or increases. It can be divided into primary, characterized by not having an underlying cause, and secondary, which can be caused by infectious agents, such as mycoplasmosis, neoplasms, drugs and transfusions. The objective of this work was to perform a clinical and laboratory analysis of a dog with immune-mediated hemolytic anemia due to Mycoplasma spp. A five-months-old female Pit Bull dog was taken to the hospital presenting vomiting, diarrhea and anorexia. On physical examination, it was possible to observe the presence of ticks and icteric mucous membranes. In hematological exams, it was possible to show morphological changes in red blood cells that characterize immune-mediated hemolytic anemia and the presence of the parasite Mycoplasma spp. In the biochemical analyzes it was observed increased serum levels of creatinine and urea. It can be concluded that the observation of Mycoplasma spp. in blood vessels associated with erythrocyte morphological changes are of relevant importance for the diagnosis of AHIM, thus assisting in the practice of a better method of therapy, contributing to the patient's prognosis.(AU)
Asunto(s)
Animales , Femenino , Perros , Enfermedades de los Perros/sangre , Enfermedades de los Perros/diagnóstico , Anemia Hemolítica/diagnóstico , Anemia Hemolítica/veterinaria , Anemia Hemolítica/sangre , Anemia Hemolítica/parasitología , Infecciones por Mycoplasma/tratamiento farmacológico , Infecciones por Mycoplasma/inmunología , Infecciones por Mycoplasma/veterinariaRESUMEN
Ehrlichiosis is a tick-borne disease highly prevalent in Brazil, and is relevant in canine clinical practice due to its high morbidity and mortality. Its clinical signs are nonspecific and its phases are acute, lasting 2 to 4 weeks; subclinical, i.e., asymptomatic; and chronic, resembling an autoimmune disease. The purpose of this study was to identify the occurrence of reactivity to Ehrlichia canis of bitches treated at the Veterinary Medical Teaching Hospital of the Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil, based on serological examination by iELISA, and to compare the hematological, biochemical, urinary protein-creatinine and urinary density profiles of reactive and non-reactive animals. This study involved solely bitches, regardless of breed, starting at 1 year of age. One hundred and thirty bitches, 1 to 16 year-old (mean age 7.02 ± 4.00), weighing 1.5 to 50 kg (mean weight 12.12 ± 10.65) were subjected to clinical examination and abdominal ultrasound. Complete blood count, biochemical measurements, urinalysis and serology for E. canis were also performed. The serum was used in the iELISA to identify immunoglobulin G (IgG), using a canine Ehrlichia Imunotest® diagnostic kit (Imunodot®, Jaboticabal, SP, Brazil) according to the manufacturer's instructions. Sixty animals (46.20%) were reactive to E. canis. According to their owners, only 5 (8.3%) of the 60 seroreactive animals had a history of tick-borne disease. The most common profile was that of mixed breed animals living with their owners, older than 7 years, who had not been treated preventatively with specific drugs against ectoparasites. Laboratory tests showed significant differences between groups in terms of total protein (TP), and calcium and urinary protein-creatinine ratio (UPC). TP and UPC were elevated in the non-reactive group, while the only significant change in the reactive group was mild hypocalcemia. In this study, 30% (18/60) of the bitches were seroreactive to E. canis and had hypocalcemia. Of these, 50% (9/18) had a UPC above 0.5. Furthermore, 66.7% (12/18) of this group with hypocalcemia also showed urine density (UD) of less than 1024. Among these 18 bitches, 5 had both alterations, i.e., UPC > 0.5 and UD < 1024. In this study, a high prevalence of bitches seroreactive to Ehrlichia canis was observed, despite the absence of clinical and/or laboratory signs indicative of the disease. In the investigation of IgG class antibodies, it is not possible to determine the exact time of infection, and titers may remain high for a period of more than 11 months, even after treatment and elimination of the bacterium. The fact that most seroreactive bitches showed no symptoms compatible with the disease either before or during the study suggests that they were in the subclinical phase of ehrlichiosis. The main reason for calcium metabolism disorders is a phosphorus imbalance, a condition that occurs in kidney diseases. Isosthenuria reflects the kidney's inability to concentrate urine. This finding may be one of the first clinical manifestations of chronic kidney disease (CKD), especially in dogs. On the other hand, the UPC ratio may increase with the progression of CKD. The presence of hypocalcemia, isosthenuria and increased UPC associated with seroreactivity suggests that infection by E. canis may be associated with the onset of CKD. Veterinarians should keep in mind the complexity of the pathophysiology of ehrlichiosis to ensure the disease is not underdiagnosed in any of its phases, thereby ensuring the correct treatment is provided. Such awareness is expected to reduce the chronicity of the disease and underlying sequelae among dogs.(AU)
Asunto(s)
Animales , Femenino , Perros , Ehrlichiosis/sangre , Ehrlichiosis/veterinaria , Enfermedades por Picaduras de Garrapatas/veterinaria , Enfermedades de los Perros/sangre , Ensayo de Inmunoadsorción Enzimática/veterinaria , Prevalencia , PerrosRESUMEN
Malignant mammary tumors in humans and bitches cause hematological disorders such as anemia, erythrocytosis, thrombocytosis, hyperproteinemia, and leucopenia. Novel studies have been conducted on the predictive and prognostic values of platelet (PLT) indices in human breast cancer (HBC). However, there is little information about the alterations in hematological parameters in canine mammary tumors (CMTs). The aims of this study were to evaluate the platelet indices and complete blood count (CBC) parameters in bitches with and without mammary tumor and to assess the above mentioned parameters with regard to histological tumor types and grades. A total of 71 bitches were enrolled in this study. The bitches in the study group were divided into 2 groups which consisted of malignant epithelial mammary tumors (group EMT; n = 43) and malignant mixed mammary tumors (group MMT; n = 12). Control group (group C) consisted of clinically and gynaecologically healthy 16 bitches. Blood samples were obtained to perform the CBC and PLT indices analysis. Histopathological examinations were carried out under a light microscope. Histological tumor types and malignancy grades were classified. The bitches with mammary tumor showed significantly increased PLT values and decreased hematocrit (HCT), hemoglobin (HGB) and mean corpuscular hemoglobin (MCH) values versus the healthy ones, regardless of the tumor type. However, in comparisons with the group C, mean platelet volume (MPV) and mean corpuscular hemoglobin concentration (MCHC) values were different only in the group MMT, while plateletcrit (PCT) and mean corpuscular volume (MCV) values were different only in the group EMT. Also white blood cell (WBC), PLT and PCT values were higher than the referenced laboratory ranges in grade 3 tumors. In the presented study, MPV was considerably correlated with PLT, platelet distribution width (PDW) and PCT. Also, PCT and PLT had high sensitivity and specificity to distinct EMT and MMT from the healthy bitches. Microcytic and hypochromic anemia occurs due to the decrease in the amount of HGB. Levels of MCV, MCH, and MCHC in the HBC group were reported to be significantly lower than in humans without breast cancer. Although anemia did not occur in EMT and MMT groups, obtained significances in the HCT, HGB, MCV, MCH, and MCHC levels between the bitches with and without mammary tumor were in line with the previous reports. In this study, WBC levels in grade 3 tumors were significantly higher than grade1 tumors (P < 0.05). Whereas levels of WBC in grade 1 and grade 2 tumors were in referenced laboratory ranges, it was higher in grade 3. Increased level of WBC in grade 3 was supposed to be due to the rise in malignancy as previously reported. Thrombocytosis was detected in 48.83% and 41.66% of the bitches in EMT and MMT groups, respectively. The higher percentage of CMTs with thrombocytosis in this study might be due to the difference in referenced upper limit of PLT in previous studies. The elapsed time between tumor formation and clinical presentation could be another influencing factor. Although PLT and PCT values were not significant according to the histological grading in this study, both parameters were found to be higher in grade 3 than the normal reference values. Further studies conducted with higher populations may lead the differences in these parameters to significance. With the support of further studies, alterations in the above mentioned parameters in bitches may contribute in the diagnosis process, management of treatment and may constitute an easy way to have an idea about the prognosis of mammary tumors.(AU)
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Animales , Femenino , Perros , Neoplasias Mamarias Animales/sangre , Enfermedades de los Perros/sangre , Recuento de Plaquetas/veterinariaRESUMEN
Canine Leishmaniasis (CanL) is a multisystemic and chronic inflammatory disease characterized by nonspecific clinical manifestations. In CanL, inflammatory cells and chemical mediators released in response to the parasite play a role in disease development and progression. Alterations on hematological parameters have been documented in CanL. These changes can also be assessed in relation to systemic inflammation caused by this disease. The circulating leukocyte counting, such as neutrophils, as well as the albumin level, are considered direct indicators of an inflammatory host environment. Several studies point to the use of biomarkers on the assistance in diagnosis and prognosis of several canine pathologies. The present study investigated the Neutrophils to Lymphocyte Ratio (NLR), Albumin to Globulin Ratio (AGR), and Neutrophils to Albumin Ratio (NAR) on systemic inflammatory response induced by Canine Leishmaniasis (CanL). For this purpose, adult dogs with confirmed diagnosis to CanL were divided into symptomatic (SD, n = 33) and asymptomatic (AD, n = 20) dogs for L. infantum and control dogs (CD, n = 20). Routine hematological and biochemical parameters were determined in blood samples using a veterinary automatic hematology and biochemical analyzers. Asymptomatic dogs (AD) had a higher number of white blood cells and neutrophils (16.48 ± 4.93; 13.41 ± 3.60, respectively) in relation to symptomatic dogs (SD) (13.54 ± 5.13; 10.42 ± 3.69, respectively) (P = 0.015 and P < 0.0001, respectively). Neutrophils to Lymphocyte Ratio (NLR) was higher in dogs with leishmaniasis (9.45 ± 3.76) than in healthy dogs (3.39 ± 1.19) (P < 0.0001). Serum total proteins (STP) and globulins increased in CanL, while albumin and AGR decreased in CanL, when compared to CD and references values to canine species. Neutrophils to Albumin Ratio (NAR) was higher in AD and SD (5.02 ± 1.14; 4.79 ± 1.07, respectively) when compared to CD (2.36 ± 0.55) (P < 0.0001). As reported in scientific researches, dogs with Leishmaniasis present alterations in circulating cell counts. Based on these data, we decided to expand this information using the NLR as a parameter in an attempt to better clarify the changes in these cells in CanL. We observed that NLR was increased on CanL in relation to healthy dogs, which could be a consequence of relative neutrophilia rather than lymphopenia. Neutrophils to Lymphocyte Ratio (NLR) is a biomarker that conveys information about inflammatory conditions. An elevated NLR can reflect an upregulated innate immune response, since neutrophils are effector cells of innate immunity and are involved in several acute and chronic inflammatory processes. Albumin is an acute phase protein that is considered an immune-inflammatory biomarker, which can be found reduced systemically in progressive inflammatory response. Serum total proteins (STP) and globulins were increased in CanL. These data are already well documented in CanL, which serum globulins are mainly associated with the increase of acute phase proteins, cytokines, and increase of specific antibodies to Leishmania infantum. Our results showed neutrophilia with hypoalbuminemia in CanL. So, in an attempt to assess the relationship of these two available markers, we used NAR calculation in order to evaluate the changes induced by CanL. In this study NAR was higher in CanL when compared to control dogs. Thus, our data indicate that NLR and NAR could be used as biomarkers in veterinary medical clinics in order to assess inflammatory profile in CanL, mainly in asymptomatic dogs. These parameters obtained from routine blood tests might be useful as cost-effective, easily accessible and helpful markers in order to distinguish the inflammatory response intensity in CanL.(AU)
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Animales , Perros , Biomarcadores/sangre , Leishmaniasis/veterinaria , Leishmania infantum , Enfermedades de los Perros/parasitología , Enfermedades de los Perros/sangre , Pruebas Hematológicas/veterinaria , Perros , Enfermedades Desatendidas/veterinariaRESUMEN
Blood transfusion is a tool capable of saving lives. Patients undergoing blood transfusion usually present several alterations in the acid-base and electrolyte balance, aggravating the condition of critically ill patients. Some studies have demonstrated haematological alterations in certain species that received whole blood transfusions, however, few studies have evaluated acid base and electrolyte changes in dogs undergoing whole blood haemotherapy. The aim of this study was to analyze clinical, hematological, blood gas and electrolyte changes in anemic dogs after whole blood transfusion. Twenty nine dogs transfused due to anemia were enrolled in the study. Donors blood was collected in a transfusion bag containing citrate phosphate adenine dextrose and stored up to 24 h. Blood collections and evaluations were made before and 24 h after the transfusion. Data distribution normality was tested by the Shapiro Wilk Test. The means of the variables were compared by paired t-test. It was observed an increase in diastolic blood pressure and a reduction in heart rate (P < 0.05). There was a not significant increase in systolic blood pressure, temperature, and a reduction in respiratory rate per minute. Erythrocyte, haemoglobin and haematocrit averages were significantly increased after blood transfusion (P < 0.05). It was observed a reduction in the mean values of pH (P < 0.05), potassium (P > 0.05) and ionized calcium (P > 0.05) and an increase in the mean partial pressure of carbon dioxide (pCO2 ) (P < 0.001), bicarbonate (P > 0.05) and sodium (P < 0.05). The probable cause of anemia was monocytic ehrlichiosis (14/29), visceral leishmaniasis (1/29), babesiosis (1/29), co-infection of Ehrlichia canis and Leishmania infantum (2/29), co-infection of E. canis and Babesia vogeli (1/29). It was not possible to determine the etiology of the anemia in ten dogs. Heart rate significantly reduced after transfusion, probably because of the increase in hematocrit, hemoglobin and erythrocyte values. It may be justified by the displacement of extravascular fluid to the intravascular space. Mean values of systolic blood pressure were slightly elevated before transfusion and remained elevated afterwards, while diastolic and mean arterial pressure increased significantly after transfusion. These changes may be due to the morbid condition and may be influenced by many other factors. Haematocrit, haemoglobin and erythrocyte values increased significantly after transfusion, according to what was observed in other studies. The significant reduction in pH and increase in pCO2 reflects the compensatory mechanism for metabolic acidosis to increase ventilation, leading to pCO2 reduction and changes in pH. The reduction in pH due to the contact of the collected blood with conservative solutions is one of the main changes thar occurs during blood storage. It was described significantly lower pH in dogs' whole blood samples stored for more than 24 h in vacutainer plastic containing CPDA-1. We may assume there was no intense pH reduction in the present study because the bags were stored for up to 24 h. Although not statistically significant, the increase of pO2 mean reflects the improvement of tissue oxygen perfusion. It was observed a significant increase in sodium ions. The mean sodium ion concentration before transfusion was very close to the maximum reference value. Hyperkalaemia was not observed, nor was there significant reduction of potassium ions after transfusion. Several studies report hyperkalaemia and transfusion-associated cardiac arrests in humans, associated with infusion of large volumes of blood. Whole blood transfusion increased erythrogram values and did not negatively affect the electrolyte or acid-base status, representing a safe and useful tool in the intensive care of small animals.(AU)
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Animales , Perros , Equilibrio Hidroelectrolítico , Transfusión Sanguínea/veterinaria , Enfermedades de los Perros/sangre , Reacción a la Transfusión/veterinaria , Anemia/veterinaria , Análisis de los Gases de la Sangre/veterinaria , PerrosRESUMEN
Brucellosis is one of the most common bacterial zoonoses worldwide affecting not only livestock and wildlife but also pets. Canine brucellosis is characterized by reproductive failure in dogs. Human Brucella canis infections are rarely reported but probably underestimated due to insufficient diagnostic surveillance. To improve diagnostics, we investigated dogs in a breeding kennel that showed clinical manifestations of brucellosis and revealed positive blood cultures. As an alternative to the time-consuming and hazardous classical identification procedures, a newly developed species-specific intact-cell matrix-assisted laser desorption/ionization-time of flight mass spectrometry analysis was applied, which allowed for rapid identification of B. canis and differentiation from closely related B. suis biovar 1. High-throughput sequencing and comparative genomics using single nucleotide polymorphism analysis clustered our isolates together with canine and human strains from various Central and South American countries in a distinct sub-lineage. Hence, molecular epidemiology clearly defined the outbreak cluster and demonstrated the endemic situation in South America. Our study illustrates that MALDI-TOF MS analysis using a validated in-house reference database facilitates rapid B. canis identification at species level. Additional whole genome sequencing provides more detailed outbreak information and leads to a deeper understanding of the epidemiology of canine brucellosis.
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Brucella canis , Brucelosis , Brotes de Enfermedades , Enfermedades de los Perros , Genoma Bacteriano , Polimorfismo de Nucleótido Simple , Animales , Brucella canis/genética , Brucella canis/metabolismo , Brucelosis/sangre , Brucelosis/epidemiología , Brucelosis/genética , Brucelosis/veterinaria , Enfermedades de los Perros/sangre , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/genética , Perros , Genómica , Secuenciación de Nucleótidos de Alto Rendimiento , América del Sur/epidemiología , Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónRESUMEN
The purpose of this study is to determine whether the brachycephalic obstructive airway syndrome (BOAS) is correlated to alterations in liver and spleen elasticity. Forty-eight brachycephalic and 22 mesocephalic dogs were submitted to a BOAS functional assessment, laboratory tests, abdominal ultrasound and liver and spleen Acoustic Radiation Force Impulse (ARFI) elastography. Dogs clinically affected by BOAS had higher values of liver stiffness (p < 0.001) than healthy dogs: medial lobes (1.57 ± 0.37 m/s), left and right lateral lobes (1.54 ± 0.50 m/s, 1.23 ± 0.28 m/s, respectively) and caudate lobe (1.28 ± 0.42 m/s). Compared to the mesocephalic group, the brachycephalic group (BOAS clinically affected and unaffected dogs) had higher spleen (2.51 ± 0.45 m/s; p < 0.001) and liver stiffness (p < 0.001): medial lobes (1.53 ± 0.37 m/s), left and right lateral lobes (1.47 ± 0.47 m/s, 1.20 ± 0.30 m/s, respectively) and caudate lobe (1.23 ± 0.40 m/s). Principal component analysis explained 70% of the variances composed by liver stiffness increase, erythrocytes and alanine aminotransferase reduction. Brachycephalic dogs had higher spleen and liver stiffness and a subacute inflammatory state, which represent another BOAS systemic effect. Consequently, these dogs can be at higher risk of hepatic disorders compared with mesocephalic dogs, similarly to humans affected by sleep apnea syndrome.
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Obstrucción de las Vías Aéreas/veterinaria , Craneosinostosis/veterinaria , Enfermedades de los Perros/diagnóstico por imagen , Hígado/diagnóstico por imagen , Bazo/diagnóstico por imagen , Obstrucción de las Vías Aéreas/sangre , Obstrucción de las Vías Aéreas/diagnóstico por imagen , Obstrucción de las Vías Aéreas/etiología , Alanina Transaminasa/sangre , Animales , Estudios de Casos y Controles , Craneosinostosis/sangre , Craneosinostosis/complicaciones , Craneosinostosis/diagnóstico por imagen , Enfermedades de los Perros/sangre , Perros , Diagnóstico por Imagen de Elasticidad , Estudios ProspectivosRESUMEN
Sarcocystis neurona, a coccidian parasite shed by opossums (Didelphis spp.) in the Americas, is the major cause of equine protozoal myeloencephalitis (EPM) and induces disease in other domestic and wild animal species, including domestic dogs. Sarcocystis cruzi, despite its low pathogenicity for cattle (intermediate hosts), is worldwide distributed and uses mostly dogs as definitive hosts. The aims of this study were to test serological reactivities of dog sera to S. neurona and S. cruzi antigens, and to investigate potential serological cross-reactivity to these parasites. Sera from 353 Brazilian dogs were obtained from rural areas in the municipality of Ilhéus, Bahia, and examined by immunofluorescent antibody tests (IFAT). Antigens used in serological reactions consisted of S. neurona merozoites from a North American strain (SN138), and bradyzoites of S. cruzi obtained from Brazilian bovine hearts, with parasite species identity confirmed by PCR and sequencing of the 18S gene of the rDNA. Seropositivity to S. neurona and to S. cruzi were detected in 3.39% (12/353) and 4.81% (17/353) of the dogs, respectively. Ten canine sera reacted solely to S. neurona and 15 serum samples reacted only to S. cruzi. Two serum samples were simultaneously positive for both parasites. Sera from 14 dogs that tested positive by IFAT (9 for S. neurona and 3 for S. cruzi) and from two dogs that were negative by IFAT for the two parasites, were examined by Western blot using S. neurona as antigen; these sera reacted to a great number of protein bands, including antigens on the 16 and 30 KDa positions, which encompass immunodominant antigens for S. neurona in horses. Western blot did not show any specific pattern for S. neurona infection/exposure using canine sera. Dogs act as definitive hosts for several Sarcocystis spp. that infect farm animals, including horses, sheep, goats, water buffaloes and pigs, and for this reason, should contain antibodies to a broad repertoire of Sarcocystis spp. antigens. In conclusion, low percentages of dogs from rural areas of Ilhéus, Bahia, were reactive to both S. neurona and S. cruzi antigens. It is possible that other Sarcocystis species, besides S. neurona and S. cruzi, might have contributed for the seropositivity observed in this study. IFAT was more specific than Western blot to differentiate canine serological reactions to S. neurona and S. cruzi antigens.
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Antígenos de Protozoos/sangre , Enfermedades de los Perros/inmunología , Sarcocystis/inmunología , Sarcocistosis/veterinaria , Animales , Brasil , Enfermedades de los Perros/sangre , Enfermedades de los Perros/parasitología , Perros , Femenino , Masculino , Sarcocistosis/sangre , Sarcocistosis/inmunología , Sarcocistosis/parasitología , Suero/parasitología , Especificidad de la EspecieRESUMEN
Piroplasm species were analyzed by molecular tools in total 31 blood samples from positive dogs, previously checked by stained slides, stored until DNA extraction between 2016 to 2018 in the laboratory Clinical Analyzes in Niterói, Rio de Janeiro. The piroplasms were identified by PCR, targeting the 18S rRNA gene and sequencing. From the total number of samples only 24 (77.4%) were positive and show adequate nucleotide sequences for interpretation with identity between 93%-100% with Babesia vogeli in compared to the sequences isolated of infected dogs from other states in Brazil deposited on GenBank. Most of dogs infected with B. vogeli had anemia (62.5%) and thrombocytopenia (95.8%). The findings of this study are compatible with previous reports in the literature and highlight B. vogeli as the most incriminated species in canine piroplasmosis in Brazil, and thrombocytopenia the hematological alteration most frequently identified in this infection. It is important to note that this is the first study involving the molecular characterization of piroplasms in the metropolitan region of Rio de Janeiro, based on PCR followed by sequencing.
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Babesia , Babesiosis , Sangre , Enfermedades de los Perros , Manejo de Especímenes , Animales , Babesia/genética , Babesiosis/sangre , Sangre/parasitología , Análisis Químico de la Sangre , Brasil , Enfermedades de los Perros/sangre , Enfermedades de los Perros/parasitología , Perros , ARN Ribosómico 18S/genética , Manejo de Especímenes/veterinariaRESUMEN
OBJECTIVE: Postoperative cognitive dysfunction (POCD) may be related to brain injury. S100B protein and neuron-specific enolase (NSE) have been investigated as potential biochemical markers of neural cell injury in animals and humans. This study aimed to investigate the association between POCD, brain injury and serum concentrations of S100B and NSE after periodontal surgery in aged dogs. STUDY DESIGN: Prospective observational animal study. ANIMALS: A total of 24 male and female dogs undergoing periodontal surgery. METHODS: Dogs were separated into two groups based on age: control group, 10 dogs ≤ 8 years and aged group, 14 dogs > 8 years. Cognitive function was measured preoperatively and on the seventh postoperative day using the Canine Cognitive Dysfunction Rating scale and the Age-Related Cognitive and Affective Disorders scale. S100B protein and NSE serum concentrations were measured before and immediately after the surgery. RESULTS: POCD was not observed after surgery in the present study. Serum concentrations of S100B and NSE were increased postoperatively in the control group but not in the aged group (p = 0.04 and 0.03, respectively). Preoperative S100B serum concentrations were significantly higher in the aged group (p = 0.01). CONCLUSIONS: There was no association between POCD and high concentrations of S100B and NSE in dogs. However, increased postoperative serum concentrations of S100B and NSE were found in the control group after surgery, an effect that may indicate neural damage. CLINICAL RELEVANCE: The results suggest that anesthesia and oral surgery are associated with higher postoperative serum concentrations of S100B and NSE in dogs ≤ 8 years old, which may indicate neural damage. Serum concentrations of S100B were elevated in aged dogs before anesthesia, a finding that might be related to chronic preoperative brain damage.
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Anestesia/veterinaria , Enfermedades de los Perros/diagnóstico , Fosfopiruvato Hidratasa/sangre , Complicaciones Cognitivas Postoperatorias/diagnóstico , Subunidad beta de la Proteína de Unión al Calcio S100/sangre , Envejecimiento , Animales , Estudios de Casos y Controles , Enfermedades de los Perros/sangre , Enfermedades de los Perros/enzimología , Perros , Femenino , Masculino , Complicaciones Cognitivas Postoperatorias/sangreRESUMEN
We evaluated the seroprevalence of Saint Louis encephalitis virus (SLEV) and West Nile virus (WNV) in dogs and cats in Córdoba, Argentina. Monotypic and heterotypic serological patterns were differentiated by means of a neutralization test. The SLEV seroprevalence in dogs was 14.6% (44/302; 100% monotypic). Two out of 94 (2.1%, 100% monotypic) cats were positive for WNV only. Four dogs (1.3%) exhibited neutralizing antibody titers against SLEV and WNV. During the study, three dogs seroconverted to SLEV. Our study demonstrates that pets were useful for detecting viral activity and could be considered as sentinels in the local surveillance of SLEV and WNV.