RESUMEN
En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Creatina Quinasa/sangre , Pruebas Inmunológicas/métodos , Creatina Quinasa , Creatina Quinasa/sangre , Creatina Quinasa/inmunología , Creatina Quinasa/aislamiento & purificación , Electroforesis en Acetato de Celulosa/normas , Electroforesis en Acetato de Celulosa/estadística & datos numéricosRESUMEN
En la práctica diaria, cuando se realiza la determinación de creatinquinasa isoenzima MB (CK-MB) por el método inmunológico, es habitual obtener resultados incongruentes: valores de CK-MB cercanos o superiores al de creatinquinasa total (CK). En el presente trabajo se informa acerca de las interferencias más frecuentes encontradas con este método en nuestro Instituto (AU)
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Anciano , Creatina Quinasa/sangre , Pruebas Inmunológicas/métodos , Creatina Quinasa/inmunología , Electroforesis en Acetato de Celulosa/normas , Electroforesis en Acetato de Celulosa/estadística & datos numéricos , Creatina Quinasa/aislamiento & purificación , Creatina Quinasa/sangre , Creatina Quinasa/diagnósticoRESUMEN
Human isonucleotidases were separated by electrophoresis on a cellulose acetate membrane. Three 5'-nucleotidase forms, NTP1, NTP2, and NTP3, were resolved with this method and quantified by densitometry. The procedure was not only simple and rapid but also sufficiently precise (between-run CV < 20%) and sensitive (detected nucleotidase fractions of > 0.5 U/L). The effects of various treatments (heat, neuraminidase, glycosidases, proteases, lectins, and detergents) on the electrophoretic pattern of 5'-nucleotidase were studied. NTP1 (mean 12% of total 5'-nucleotidase, SD 5%), NTP2 (mean 30%, SD 8%), and NTP3 (mean 58%, SD 8%) were found in all normal persons studied. The increase in total 5'-nucleotidase in patients with hepatobiliary disease was mainly due to the NTP1 isoform.
Asunto(s)
5'-Nucleotidasa/aislamiento & purificación , Electroforesis en Acetato de Celulosa , Isoenzimas/aislamiento & purificación , 5'-Nucleotidasa/análisis , 5'-Nucleotidasa/sangre , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Enfermedades de los Conductos Biliares/enzimología , Electroforesis en Acetato de Celulosa/estadística & datos numéricos , Endopeptidasas/farmacología , Femenino , Glicósido Hidrolasas/farmacología , Calor , Humanos , Isoenzimas/análisis , Isoenzimas/sangre , Hepatopatías/enzimología , Masculino , Persona de Mediana Edad , Neuraminidasa/farmacología , Octoxinol/farmacología , Sensibilidad y Especificidad , Distribución Tisular , Aglutininas del Germen de Trigo/farmacologíaRESUMEN
We adapted the electrophoretic method of bone alkaline phosphatase (ALP) determination using neuraminidase from Vibrio cholerae to separate bone and liver ALP on cellulose acetate membrane. Treatment of separator plus serum (1:8, neuraminidase 111 U/l in final) for 10 min at room temperature (25 +/- 1 degree C) and subsequent electrophoresis made it possible to quantify bone ALP activity simply and rapidly. The precision of the data was at the level of CV of 1.6% (within-day) and 4.7% (day-to-day), with recovery rates of 97-103%. The normal range of bone ALP activity depended on age and sex. Seventy-eight diabetes mellitus (DM) patients, excluding those with renal failure, were divided into two groups of those with and without osteopenia with matching of age (+/- 3 years) and sex. Bone ALP (P < 0.001) and total ALP (P < 0.05) activities and urine calcium/creatinine ratio (P < 0.05) were significantly higher in DM with osteopenia than in DM without osteopenia. Therefore, bone formation and absorption may be accelerated in DM with osteopenia in comparison with DM without osteopenia.