RESUMEN
The nonnutritive sweeteners cyclooctylsulfamate and 4-methylcyclohexylsulfamate were fed separately to female Wistar albino rats, and the urine was examined for the possible metabolites cyclooctylamine, cyclooctanone, cyclooctanol, 4-methylcyclohexylamine, 4-methylcyclohexanone, and cis- and trans-4-methylcyclohexanols. The average percent conversions to cyclooctylamine, cyclooctanone, and cyclooctanol were 0.127, 0.08, and 0.092, respectively. The average percent conversions to 4-methylcyclohexylamine and 4-methylcyclohexanone were 0.007 and 0.0013, respectively. No cis- or trans-4-methylcyclohexanol metabolites were found. With cyclooctylsulfamate, 42% was recovered unchanged from the urine. Cyclooctyl- and cycloheptylsulfamates were metabolized to a greater extent than cyclopentylsulfamate, which, in turn, was metabolized to a greater extent than cyclohexylsulfamate (cyclamate) and 4-methylcyclohexylsulfamate.
Asunto(s)
Ciclamatos/metabolismo , Edulcorantes/metabolismo , Animales , Biotransformación , Cromatografía de Gases , Ciclamatos/orina , Femenino , Ratas , Espectrofotometría , Edulcorantes/orinaRESUMEN
The nonnutritive sweetener cycloheptylsulfamate was administered orally to rabbits and rats. The urine of each species was separately collected for 3 days and examined for the metabolites cycloheptylamine, cycloheptanone, and cycloheptanol and for cycloheptylsulfamate. A previously tested GLC method was adapted for the determination of the metabolites. Cycloheptylsulfamate was assayed by hydrolysis and subsequent measurement of the absorbance of the product formed (lambdamax=489 nm) by the liberated amine with p-benzoquinone. The conversions to the metabolites were 0.276, 0.390, and 0.170%, respectively, in rabbits and 0.064, 0.022, and 0.017%, respectively, in rats.
Asunto(s)
Cicloheptanos/orina , Edulcorantes/orina , Animales , Biotransformación , Cromatografía de Gases , Ciclamatos , Femenino , Luz , Conejos , Ratas , Espectrofotometría , Ácidos Sulfónicos/orinaRESUMEN
The nonnutritive sweetener sodium cyclopentylsulfamate was fed to Wistar albino rats and New Zealand White rabbits. Urine was collected for 3 days after feeding, combined, and examined for the metabolites cyclopentylamine, cyclopentanone, and cyclopentanol and for sodium cyclopentylsulfamate was assayed by hydrolysis in acidified dioxane-water and subsequent measurement of the absorbance of the product formed (lambdamax = 490 nm) by the liberated amine with p-benzoquinone. The average conversion to cyclopentylamine, cyclopentanone, and cyclopentanol was 0.103, 0.171, and 0.054% in the rabbit and 0.057, 0.016, and 0.008% in the rat, respectively.