RESUMEN

A Gram-negative, halophilic, heterotrophic, rod-shaped, non-spore-forming bacterium (SV525T) was isolated from the sediment of a hypersaline lake located at 4600 m above sea level (Laguna Vilama, Argentina). Strain SV525T was strictly aerobic and formed pink-to-magenta colonies. Growth occurred at 10­35 °C (optimum 25­30 °C), at pH levels 6.0­8.5 (optimum 7.0) and at NaCl concentrations of 7.5­25 % (w/v) with an optimum at 10­15 % (w/v). The strain required sodium and magnesium but not potassium ions for growth. Grows with tryptone, or Bacto Peptone as sole carbon and energy source and requires yeast extract for growth. It produced catalase and oxidase. The predominant ubiquinone was Q-8 and the major fatty acids comprised C18:1 ω7c, C16:0 and C18:0. The DNA G+C content was 60.4 mol% and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and a phosphoglycolipid. Phylogenetic analysis based on 16S rRNA gene indicated that strain SV525T belongs to the family Ectothiorhodospiraceae within the class Gammaproteobacteria. On the basis of phylogenetic and phenotypic data, SV525T represents a novel genus and species, for which the name Halopeptonella vilamensis gen. nov., sp. nov. is proposed. The type strain is SV525T (=DSM 21056T =JCM 16388T =NCIMB 14596T).

Asunto(s)

Ectothiorhodospiraceae/aislamiento & purificación , Filogenia , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Composición de Base , ADN Bacteriano/química , ADN Bacteriano/genética , Ectothiorhodospiraceae/clasificación , Ectothiorhodospiraceae/genética , Ectothiorhodospiraceae/metabolismo , ARN Ribosómico 16S/genética