Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Más filtros











Base de datos
Intervalo de año de publicación
1.
Extremophiles ; 20(1): 19-25, 2016 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-26475627

RESUMEN

A Gram-negative, halophilic, heterotrophic, rod-shaped, non-spore-forming bacterium (SV525T) was isolated from the sediment of a hypersaline lake located at 4600 m above sea level (Laguna Vilama, Argentina). Strain SV525T was strictly aerobic and formed pink-to-magenta colonies. Growth occurred at 10­35 °C (optimum 25­30 °C), at pH levels 6.0­8.5 (optimum 7.0) and at NaCl concentrations of 7.5­25 % (w/v) with an optimum at 10­15 % (w/v). The strain required sodium and magnesium but not potassium ions for growth. Grows with tryptone, or Bacto Peptone as sole carbon and energy source and requires yeast extract for growth. It produced catalase and oxidase. The predominant ubiquinone was Q-8 and the major fatty acids comprised C18:1 ω7c, C16:0 and C18:0. The DNA G+C content was 60.4 mol% and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and a phosphoglycolipid. Phylogenetic analysis based on 16S rRNA gene indicated that strain SV525T belongs to the family Ectothiorhodospiraceae within the class Gammaproteobacteria. On the basis of phylogenetic and phenotypic data, SV525T represents a novel genus and species, for which the name Halopeptonella vilamensis gen. nov., sp. nov. is proposed. The type strain is SV525T (=DSM 21056T =JCM 16388T =NCIMB 14596T).


Asunto(s)
Ectothiorhodospiraceae/aislamiento & purificación , Filogenia , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Composición de Base , ADN Bacteriano/química , ADN Bacteriano/genética , Ectothiorhodospiraceae/clasificación , Ectothiorhodospiraceae/genética , Ectothiorhodospiraceae/metabolismo , ARN Ribosómico 16S/genética
2.
Int J Syst Evol Microbiol ; 65(10): 3641-3644, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26198437

RESUMEN

Analysis of phylogenomic metrics of a recently released draft genome sequence of the halotolerant, acidophile 'Thiobacillus prosperus' DSM 5130 indicates that it is not a member of the genus Thiobacillus within the class Betaproteobacteria as originally proposed. Based on data from 16S rRNA gene phylogeny, and analyses of multiprotein phylogeny and average nucleotide identity (ANI), we show that it belongs to a new genus within the family Ectothiorhodospiraceae, for which we propose the name Acidihalobacter gen. nov. In accordance, it is proposed that 'Thiobacillus prosperus' DSM 5130 be named Acidihalobacter prosperus gen. nov., sp. nov. DSM 5130T ( = JCM 30709T) and that it becomes the type strain of the type species of this genus.


Asunto(s)
Ectothiorhodospiraceae/clasificación , Filogenia , Thiobacillus/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Datos de Secuencia Molecular , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN
3.
FEMS Microbiol Ecol ; 74(3): 510-22, 2010 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-20868378

RESUMEN

Phototrophic bacteria are important primary producers of salt lakes in the Salar de Atacama and at times form visible mass developments within and on top of the lake sediments. The communities of phototrophic bacteria from two of these lakes were characterized by molecular genetic approaches using key genes for the biosynthesis of the photosynthetic apparatus in phototrophic purple bacteria (pufLM) and in green sulfur bacteria (fmoA). Terminal restriction fragment length polymorphism of the pufLM genes indicated high variability of the community composition between the two lakes and subsamples thereof. The communities were characterized by the dominance of a novel, so far undescribed lineage of pufLM containing bacteria and the presence of representatives related to known halophilic Chromatiaceae and Ectothiorhodospiraceae. In addition, the presence of BChl b-containing anoxygenic phototrophic bacteria and of aerobic anoxygenic bacteria was indicated. Green sulfur bacteria were not detected in the environmental samples, although a bacterium related to Prosthecochloris indicum was identified in an enrichment culture. This is the first comprehensive description of phototrophic bacterial communities in a salt lake of South America made possible only due to the application of the functional pufLM genes.


Asunto(s)
Gammaproteobacteria/genética , Filogenia , Microbiología del Agua , Proteínas Bacterianas/genética , Chile , Chromatiaceae/clasificación , Chromatiaceae/genética , Dermatoglifia del ADN , ADN Bacteriano/genética , Ectothiorhodospiraceae/clasificación , Ectothiorhodospiraceae/genética , Gammaproteobacteria/clasificación , Genes Bacterianos , Complejos de Proteína Captadores de Luz/genética , Fotosíntesis , Proteínas del Complejo del Centro de Reacción Fotosintética/genética , Procesos Fototróficos , Polimorfismo de Longitud del Fragmento de Restricción , Salinidad
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA