RESUMEN
Various types of endoscopic biopsy forceps have been investigated in dogs. The Multibite (MB) are multiple-bite forceps that collect up to four tissue specimens in a single pass through the endoscope, reducing procedure time. The authors prospectively investigated its clinical utility by comparing procedure times and diagnostic quality of samples obtained with the MB to that of biopsies performed with a single-bite forceps (SB), in 21 dogs with gastrointestinal disorders. When comparing the depth, crush artefacts and diagnostic adequacy of the gastric and duodenal biopsies, there was no significant difference between the MB and SB forceps. The procedure time was significantly longer with the MB. There was no learning curve effect, and there were no reported adverse events. This study failed to demonstrate any significant clinical advantage associated with the use of the Multibite forceps over that of conventional disposable SB forceps.
Asunto(s)
Enfermedades de los Perros/patología , Enfermedades Gastrointestinales/veterinaria , Manejo de Especímenes/veterinaria , Instrumentos Quirúrgicos/veterinaria , Animales , Biopsia/instrumentación , Biopsia/veterinaria , Perros , Duodenoscopía/instrumentación , Duodenoscopía/veterinaria , Femenino , Enfermedades Gastrointestinales/patología , Masculino , Estudios Prospectivos , Manejo de Especímenes/instrumentaciónRESUMEN
OBJECTIVE: The effect of premedication with butorphanol or methadone on ease of endoscopic duodenal intubation. STUDY DESIGN: Prospective, randomized, blinded clinical trial. ANIMALS: A group of 20 client-owned dogs. METHODS: Dogs were assigned randomly to be administered intravenous (IV) premedication with either butorphanol (0.4 mg kg-1) or methadone (0.3 mg kg-1). General anaesthesia was induced with propofol to effect and maintained with isoflurane in 100% oxygen. Sedation score 20 minutes after premedication administration and induction dose of propofol were recorded. Heart rate, mean arterial pressure, haemoglobin oxygen saturation, respiratory rate and end-tidal isoflurane concentration were recorded every 5 minutes. Spontaneous lower oesophageal and pyloric sphincter opening, presence of gastro-oesophageal and duodeno-gastric reflux, antral peristaltic contractions and response to endoscopy were recorded as yes or no. Ease of duodenal intubation (EDI) was graded on a scale ranging from 1 (immediate entry with minimal manoeuvring required) to 4 (no entry after 2 minutes). Time (seconds) from the start of pyloric intubation to successfully entering the duodenum was recorded. RESULTS: Median EDI score [3 ± 1 (butorphanol), 4 ± 1 (methadone), p = 0.035], time [65 ± 36 seconds (butorphanol), 120 ± 38 seconds (methadone), p = 0.028] and number of dogs with spontaneous pyloric sphincter opening [7/10 (butorphanol), 2/10 (methadone), p = 0.035] differed between groups. No other significant differences were found. CONCLUSIONS AND CLINICAL RELEVANCE: In these clinical cases, duodenal intubation was performed with greater ease, shorter time and more frequent spontaneous opening of the pyloric sphincter after premedication with butorphanol in comparison to methadone. The use of butorphanol facilitated the passage of the endoscope and is therefore recommended for premedication prior to upper gastrointestinal tract endoscopy.
Asunto(s)
Anestesia General/veterinaria , Butorfanol , Sedación Profunda/veterinaria , Duodenoscopía/veterinaria , Hipnóticos y Sedantes , Intubación Intratraqueal/veterinaria , Metadona , Medicación Preanestésica/veterinaria , Anestesia General/métodos , Animales , Sedación Profunda/métodos , Perros , Duodenoscopía/métodos , Femenino , Intubación Intratraqueal/métodos , Masculino , Medicación Preanestésica/métodosRESUMEN
OBJECTIVE To assess the immunogenicity of thermostable live-attenuated rabies virus (RABV) preserved by vaporization (PBV) and delivered to the duodenal mucosa of a wildlife species targeted for an oral vaccination program. ANIMALS 8 gray foxes (Urocyon cinereoargenteus). PROCEDURES Endoscopy was used to place RABV PBV (n = 3 foxes), alginate-encapsulated RABV PBV (3 foxes), or nonpreserved RABV (2 foxes) vaccine into the duodenum of foxes. Blood samples were collected weekly to monitor the immune response. Saliva samples were collected weekly and tested for virus shedding by use of a conventional reverse-transcriptase PCR assay. Foxes were euthanized 28 days after vaccine administration, and relevant tissues were collected and tested for presence of RABV. RESULTS 2 of 3 foxes that received RABV PBV and 1 of 2 foxes that received nonpreserved RABV seroconverted by day 28. None of the 3 foxes receiving alginate-encapsulated RABV PBV seroconverted. No RABV RNA was detected in saliva at any of the time points, and RABV antigen or RNA was not detected in any of the tissues obtained on day 28. None of the foxes displayed any clinical signs of rabies. CONCLUSIONS AND CLINICAL RELEVANCE Results for this study indicated that a live-attenuated RABV vaccine delivered to the duodenal mucosa can induce an immune response in gray foxes. A safe, potent, thermostable RABV vaccine that could be delivered orally to wildlife or domestic animals would enhance current rabies control and prevention efforts.
Asunto(s)
Duodeno , Zorros , Inmunogenicidad Vacunal , Vacunas Antirrábicas/inmunología , Administración Oral , Animales , Animales Salvajes , Antígenos Virales , Duodenoscopía/veterinaria , Duodeno/inmunología , Femenino , Mucosa Intestinal/inmunología , Masculino , Vacunas Antirrábicas/administración & dosificación , Vacunas Atenuadas/inmunología , VolatilizaciónRESUMEN
OBJECTIVES: This study aimed to evaluate the agreement between microscopic and molecular testing for differentiating feline intestinal bowel disease and small cell alimentary lymphoma in duodenal endoscopic biopsies. METHODS: Four different diagnostic methods (cytology, histology, immunohistochemistry and clonality) were sequentially applied to 77 cases of feline chronic enteropathies. The agreement between the different diagnostic methods was calculated and survival data were obtained to assess the most reliable method for predicting outcome. RESULTS: Seventy-seven cases were included in the study. On multivariate survival analysis, only the clonality-based diagnosis of lymphoma was significantly associated with poor survival, with a risk of enteropathy-related death 2·8 times higher. By comparing the other tests with clonality, specificity was high (87 to 97%), whereas sensitivity was 36·8% for cytology, 39·5% for histology, 63·2% for immunohistochemistry, resulting in an overall accuracy of 62·3, 68·8 and 80·5%, respectively. CLINICAL SIGNIFICANCE: Clonality analysis can consistently increase the possibility of correctly and early diagnosing small cell lymphoma on endoscopic biopsies. Histological suspicion of alimentary lymphoma, even if not confirmed by clonality, should never be ignored, as it may represent a debutant form of lymphoma or it may later progress to lymphoma.
Asunto(s)
Enfermedades de los Gatos/diagnóstico , Enfermedades Inflamatorias del Intestino/veterinaria , Neoplasias Intestinales/veterinaria , Linfoma/veterinaria , Animales , Enfermedades de los Gatos/patología , Gatos , Diagnóstico Diferencial , Duodenoscopía/veterinaria , Duodeno/patología , Femenino , Enfermedades Inflamatorias del Intestino/diagnóstico , Enfermedades Inflamatorias del Intestino/patología , Neoplasias Intestinales/diagnóstico , Neoplasias Intestinales/patología , Linfoma/diagnóstico , Linfoma/patología , Masculino , Sensibilidad y Especificidad , Manejo de EspecímenesRESUMEN
BACKGROUND: Endoscopy is performed for direct inspection of the mucosa and acquisition of biopsies in dogs with inflammatory bowel disease (IBD). AIM: To evaluate the interobserver agreement in the endoscopic assessment of duodenal mucosa in dogs with IBD. METHODS: Thirty-five archived endoscopic images of grossly normal (n = 6) and inflamed (n = 29) duodenal mucosa were displayed to 3 expert and 5 trainee endoscopists. Each image was assessed independently by endoscopists for mucosal abnormalities using established indices (of hyperemia, granularity, friability, lymphatic dilatation, and erosions) or interpreted as normal mucosa (trial 1). A repeated trial (trial 2) was performed with the same images presented in random order 1 month later, and accompanied by a visual template. RESULTS: There was slight interobserver agreement in initial mucosal assessment for expert and trainee endoscopists in trial 1 (kappa ≤ 0.02, P > .05). Interobserver agreement improved in trial 2 for both expert and trainee endoscopists (kappa = 0.2, P > .05) for experts and (P < .05) for trainees. There was a significant (P < .01) improvement in trainee endoscopy scores of lesions from trial 1 to trial 2. Regression analysis showed a significant (P < .01) difference between expert versus trainee endoscopy scores in trial 1. Repeat lesion assessment aided by use of a visual template (trial 2) improved the overall scores of trainee endoscopists to near that of expert endoscopists (P = .06). CONCLUSIONS AND CLINICAL IMPORTANCE: Interobserver agreement of IBD mucosal appearance from endoscopic findings benefitted from operator experience.
Asunto(s)
Enfermedades de los Perros/diagnóstico , Duodenoscopía/veterinaria , Duodeno/patología , Enfermedades Inflamatorias del Intestino/veterinaria , Animales , Enfermedades de los Perros/patología , Perros , Femenino , Enfermedades Inflamatorias del Intestino/diagnóstico , Enfermedades Inflamatorias del Intestino/patología , Mucosa Intestinal/patología , Masculino , Variaciones Dependientes del ObservadorRESUMEN
This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from cats responded to at least one compound. In duodenal biopsies obtained from dogs, the rate of overall response ranged from 87.5% (healthy individuals; n = 8), to 63.6% (animals exhibiting clinical signs of gastrointestinal disease and histopathological unremarkable duodenum; n = 15), and 32.1% (animals exhibiting clinical signs of gastrointestinal diseases and moderate to severe histopathological lesions; n = 28). Detailed information regarding the magnitude and duration of the response are provided. The adapter-modified Ussing chamber enables investigation of the absorptive and secretory capacity of endoscopically obtained duodenal biopsies from cats and dogs and has the potential to become a valuable research tool. The response of samples was correlated with histopathological findings.
Asunto(s)
Biopsia/veterinaria , Gatos/fisiología , Perros/fisiología , Duodenoscopía/veterinaria , Duodeno/fisiología , Animales , Enfermedades de los Gatos/fisiopatología , Enfermedades de los Perros/fisiopatología , Enfermedades Duodenales/fisiopatología , Duodeno/fisiopatologíaRESUMEN
This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from cats responded to at least one compound. In duodenal biopsies obtained from dogs, the rate of overall response ranged from 87.5% (healthy individuals; n = 8), to 63.6% (animals exhibiting clinical signs of gastrointestinal disease and histopathological unremarkable duodenum; n = 15), and 32.1% (animals exhibiting clinical signs of gastrointestinal diseases and moderate to severe histopathological lesions; n = 28). Detailed information regarding the magnitude and duration of the response are provided. The adapter-modified Ussing chamber enables investigation of the absorptive and secretory capacity of endoscopically obtained duodenal biopsies from cats and dogs and has the potential to become a valuable research tool. The response of samples was correlated with histopathological findings.
Asunto(s)
Animales , Biopsia/veterinaria , Enfermedades de los Gatos/fisiopatología , Gatos/fisiología , Enfermedades de los Perros/fisiopatología , Perros/fisiología , Enfermedades Duodenales/fisiopatología , Duodenoscopía/veterinaria , Duodeno/fisiologíaRESUMEN
OBJECTIVE: To determine the incidence of bacteremia, as detected by routine methods for bacterial culture of blood samples, following routine endoscopic biopsy of the stomach and duodenum in healthy research dogs and to determine whether treatment with omeprazole administration affected the incidence of bacteremia. ANIMALS: 8 healthy purpose-bred research dogs. PROCEDURES: All dogs underwent gastroduodenoscopy with biopsy at 4 points: twice prior to treatment with omeprazole, once following 15 days of omeprazole treatment (20 mg, PO, q 12 h), and once 14 days after treatment ceased. Dogs had a mean ± SD body weight of 18.6 ± 2.0 kg. Blood samples were aseptically obtained at 3 points during each procedure (before, immediately following, and 24 hours after endoscopy), and routine aerobic and anaerobic bacterial culture of blood was performed. RESULTS: 96 cultures were attempted for each culture method, yielding positive results of aerobic culture for 2 dogs at separate time points and no positive results of anaerobic culture. CONCLUSIONS AND CLINICAL RELEVANCE: Routine gastrointestinal endoscopy with biopsy in healthy dogs did not result in a detectable bacteremia in most dogs. Treatment with the gastric acid-suppressing medication omeprazole did not affect the incidence of bacteremia as detected via standard techniques.
Asunto(s)
Bacteriemia/veterinaria , Biopsia/efectos adversos , Enfermedades de los Perros/epidemiología , Duodenoscopía/efectos adversos , Gastroscopía/efectos adversos , Omeprazol/efectos adversos , Inhibidores de la Bomba de Protones/efectos adversos , Animales , Bacteriemia/epidemiología , Bacteriemia/microbiología , Biopsia/veterinaria , Enfermedades de los Perros/microbiología , Perros , Duodenoscopía/veterinaria , Femenino , Gastroscopía/veterinaria , Incidencia , Masculino , Estudios ProspectivosRESUMEN
BACKGROUND: Nonsteroidal anti-inflammatory drugs frequently cause gastrointestinal (GI) injury. Zinc-L-carnosine has antioxidant, anti-inflammatory, mucosal protective, and healing properties in rodent models and in some human studies of GI injury. HYPOTHESIS: The combination of zinc-L-carnosine and vitamin E attenuates aspirin-induced gastroduodenal mucosal injury. ANIMALS: Eighteen healthy random-source Foxhound dogs. METHODS: In this randomized, double-blinded, placebo-controlled study dogs were treated with placebo (n = 6; 0X group), 30 mg/30 IU (n = 6; 1X group), or 60 mg/60 IU (n = 6; 2X group) zinc-L-carnosine/vitamin E orally every 12 hours for 35 days. Between Day 7 and 35, GI mucosal lesions were induced with aspirin (25 mg/kg p.o. q8h). Mucosal injury lesions (hemorrhage, erosion, and ulcer) were assessed by gastroduodenoscopy on Days 14, 21, and 35 with a 12-point scoring scale. RESULTS: At baseline (Day -1) gastroscopy scores were not significantly different between groups (mean ± SD: 0X, 4.4 ± 0.8; group 1X, 4.4 ± 0.6; group 2X, 4.2 ± 0.3; P= .55). Gastroscopy scores increased significantly in all groups between Day -1 and Days 14, 21, and 35 (P < .0001). On Day 35, gastroscopy scores were 29.2 ± 5.2 (0X), 27.3 ± 3.7 (1X), and 28.6 ± 3.3 (2X). Mean gastroscopy scores were not significantly different among treatment groups on any of the days (P = .61). CONCLUSIONS AND CLINICAL IMPORTANCE: Administration of the combination of zinc-L-carnosine and vitamin E at 1X or 2X dosing did not attenuate aspirin-induced gastroduodenal mucosal injury.
Asunto(s)
Antiinflamatorios no Esteroideos/efectos adversos , Antiulcerosos/uso terapéutico , Aspirina/efectos adversos , Carnosina/análogos & derivados , Enfermedades de los Perros/tratamiento farmacológico , Enfermedades Gastrointestinales/tratamiento farmacológico , Compuestos Organometálicos/uso terapéutico , Tocoferoles/uso terapéutico , Animales , Antiinflamatorios no Esteroideos/uso terapéutico , Aspirina/uso terapéutico , Peso Corporal/efectos de los fármacos , Peso Corporal/fisiología , Carnosina/uso terapéutico , Enfermedades de los Perros/inducido químicamente , Perros , Método Doble Ciego , Duodenoscopía/veterinaria , Ingestión de Alimentos/efectos de los fármacos , Ingestión de Alimentos/fisiología , Femenino , Mucosa Gástrica/efectos de los fármacos , Enfermedades Gastrointestinales/inducido químicamente , Masculino , Proyectos Piloto , Estadísticas no Paramétricas , Compuestos de Zinc/uso terapéuticoRESUMEN
OBJECTIVE: To test the hypothesis that intestinal pathologic changes are often concurrent with gastric pathologic changes in dogs and to characterize the historical, physical, clinicopathologic, imaging, and endoscopic findings in dogs with gastric histopathologic abnormalities. DESIGN: Retrospective case series. ANIMALS: 67 dogs with gastric histopathologic abnormalities. PROCEDURES: Medical records from dogs that had undergone gastrotomy, gastroduodenoscopy, or gastroscopy between September 2002 and September 2007 were identified. Dogs were included in the study when histopathologic abnormalities were detected during evaluation of gastric tissue sections. History, clinical examination findings, results of diagnostic tests, diagnoses, treatments, and outcome were recorded for each dog. RESULTS: 67 dogs with gastric histopathologic abnormalities were included in the study. The most frequent clinical sign recorded was vomiting (36/67 [53.7%] dogs). The most common biochemical abnormality recorded was panhypoproteinemia (27/64 [42.2%] dogs). Lymphoplasmacytic gastritis was the most frequent histopathologic finding recorded (34/67 [50.7%] dogs). For dogs in which both intestinal biopsy specimens and gastric biopsy specimens were collected, concurrent pathologic changes were recorded in 43 of 60 (71.7%) dogs. CONCLUSIONS AND CLINICAL RELEVANCE: Results of this study suggested that intestinal pathologic changes are commonly concurrent in dogs with gastric pathologic changes. This supports the practice of collecting both gastric and duodenal biopsy specimens every time gastroduodenoscopy is performed. Lymphoplasmacytic gastritis was the most commonly recorded gastric histopathologic finding and was often of minimal or mild severity.
Asunto(s)
Enfermedades de los Perros/patología , Gastropatías/veterinaria , Estómago/patología , Animales , Biopsia/veterinaria , Perros , Duodenoscopía/veterinaria , Femenino , Gastroscopía/veterinaria , Enfermedades Intestinales/complicaciones , Enfermedades Intestinales/patología , Enfermedades Intestinales/veterinaria , Intestinos/patología , Masculino , Estudios Retrospectivos , Estómago/cirugía , Gastropatías/complicaciones , Gastropatías/patologíaRESUMEN
The usefulness of endoscopic examination for the diagnosis and treatment of chronic enteritis in dogs was determined in this study. It was demonstrated, based on a clinical case, that endoscopy is essential for IBD diagnosis in dogs. Endoscopy also helps to estimate the efficiency of the treatment strategy applied. Nonspecific clinical examination results as well as a predominating lack of deviation in laboratory parameters make endoscopy the basic examination technique in mild and moderately advanced forms of chronic enteritis in the dog.
Asunto(s)
Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/terapia , Enfermedades Inflamatorias del Intestino/veterinaria , Animales , Diagnóstico Diferencial , Enfermedades de los Perros/patología , Perros , Duodenoscopía/veterinaria , Enfermedades Inflamatorias del Intestino/diagnóstico , Enfermedades Inflamatorias del Intestino/terapiaRESUMEN
Nonsteroidal anti-inflammatory drugs (NSAIDs) induce gastric ulcers due to inhibition of prostaglandin production. Prostaglandins have an influence on physiological gastrointestinal motility, but the relationships between NSAID-induced gastric ulcer, gastrointestinal motility and motilin are unknown. Fifteen dogs were allocated randomly to three groups in which either gelatin, meloxicam or indomethacin was administered. Fecal occult blood and gastrointestinal motility were monitored continuously for 6 days. In addition, analyses of the plasma motilin concentration, gastrointestinal endoscopy and gastric emptying, and detection of motilin cells were performed. Gastrointestinal motility was disturbed in the indomethacin group, presenting as disappearance of interdigestive migrating contractions (IMCs) 3 days before gastric ulcers were detected. Delayed gastric emptying and hypermotilinemia were observed significantly more often in the indomethacin group compared with the other groups. Motilin cell-crypt/villi ratio in the indomethacin group significantly decreased in the duodenum and jejunum, compared with the other groups. No significant changes in any tests were observed in the meloxicam group, when compared with the gelatin group. These findings suggest that the disturbance of IMCs caused by hypermotilinemia, with changes in motilin cell distribution, and delayed gastric emptying induced by indomethacin may contribute to the development of gastric ulcers.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Motilidad Gastrointestinal/efectos de los fármacos , Indometacina/farmacología , Tiazinas/farmacología , Tiazoles/farmacología , Animales , Antiinflamatorios no Esteroideos/efectos adversos , Perros , Duodenoscopía/veterinaria , Duodeno/efectos de los fármacos , Duodeno/metabolismo , Indometacina/efectos adversos , Yeyuno/efectos de los fármacos , Yeyuno/metabolismo , Masculino , Meloxicam , Motilina/metabolismo , Úlcera Gástrica/inducido químicamente , Tiazinas/efectos adversos , Tiazoles/efectos adversosRESUMEN
The effects of surgical procedures on intestinal motility are still under debate. In order to quantify the effects of duodenotomy on duodenal motility, the present study used the electric impedance technique (IMP). Ten pigs (32-40 kg) were instrumented under general anaesthesia with a central venous catheter (CVC) and a percutaneous enterogastrostomy (PEG). Duodenal phases I-III and the duration of the migrating motor complex (MMC) were measured by an IMP catheter, which was introduced non-invasively into the proximal duodenum via the PEG by endoscopy, or through surgical placement in an invasive manner via duodenotomy. There were no significant differences in the length of interdigestive phases I-III between the groups. In tendency, pigs after laparotomy and duodenotomy demonstrated slightly prolonged median duration of phase I (P = 0.1) and phase III (P = 0.7), while phase II (P = 0.1) and the MMC cycle length (P = 0.6) seemed to be shortened as compared to pigs of the PEG group. In conclusion, simple laparotomy with duodenotomy did not affect the proximal duodenal motility activity measured by the impedance technique.
Asunto(s)
Duodeno/fisiología , Duodeno/cirugía , Motilidad Gastrointestinal/fisiología , Porcinos/fisiología , Porcinos/cirugía , Animales , Duodenoscopía/veterinaria , Impedancia Eléctrica , Gastroscopía/veterinaria , Ileus/etiología , Ileus/veterinaria , Masculino , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/veterinaria , Enfermedades de los Porcinos/etiologíaRESUMEN
To investigate the effects of propofol and fentanyl on the postprandial duodenal motility the intraluminal impedance technique was used. Six pigs were instrumented with a central venous catheter, a percutaneous enterogastrostomy (PEG), and an impedance catheter, which was introduced via the PEG into the duodenum through endoscopy. Over the following 3 d, duodenal motility was measured for 8-hour periods. Measurements were taken on each subject under 3 different sets of conditions: in the conscious unrestrained pig, during propofol sedation, and during sedation with propofol-fentanyl. Both, after morning feeding and during gastric nutrition via the PEG, duodenal feeding patterns and duodenal phase II of the migrating motor cycle were shortened during propofol and propofol-fentanyl sedation. In contrast, the duration of phase I was prolonged by propofol and propofol-fentanyl. In conclusion, either propofol or propofolfentanyl sedation shortens duodenal feeding patterns, as well as phase II of the migrating motor cycle.
Asunto(s)
Fentanilo/farmacología , Motilidad Gastrointestinal/efectos de los fármacos , Hipnóticos y Sedantes/farmacología , Propofol/farmacología , Porcinos/fisiología , Animales , Duodenoscopía/veterinaria , Duodeno/efectos de los fármacos , Duodeno/fisiología , Impedancia Eléctrica , Motilidad Gastrointestinal/fisiología , Masculino , Distribución AleatoriaRESUMEN
This study assessed the gastroduodenal safety profile of licofelone, a new nonsteroidal anti-inflammatory drug with dual inhibitory activity against 5-lipoxygenase and cyclo-oxygenase (COX), by using endoscopic evaluations and by comparing licofelone to rofecoxib, a selective COX-2 inhibitor. Twenty-one dogs underwent blinded gastroduodenoscopies, during which the mucosa of the gastroduodenal tract was assessed and scored. Blood analyses were monitored on days 0 (baseline), 14, 28, 42, and 56. Examinations to detect fecal occult blood were performed daily. Dogs were randomly assigned to three groups that received either a placebo, licofelone at a dose of 2.5 mg/kg twice daily, or rofecoxib at a dose of 0.5 mg/kg daily, respectively. Significant differences between the groups in gastric (P = 0.003), duodenal (P = 0.009), and gastroduodenal (P = 0.002) endoscopic lesion scores were observed at day 56. Rofecoxib-treated dogs had more lesions in all areas when compared with placebo-treated dogs, more duodenal lesions when compared with licofelone-treated dogs and more lesions than they had at baseline. In contrast to licofelone, rofecoxib was found to induce significant gastric and gastroduodenal lesions in dogs that lacked pre-existing lesions at baseline. Blood analyses and fecal examinations did not reveal abnormalities in any of the experimental groups. Treatment with licofelone was well tolerated and was shown to be safer than rofecoxib in terms of upper gastrointestinal damage. In this way, this study demonstrates the gastroduodenal safety profile of licofelone for chronic treatment.
Asunto(s)
Acetatos/farmacología , Antiinflamatorios no Esteroideos/farmacología , Lactonas/farmacología , Pirroles/farmacología , Sulfonas/farmacología , Acetatos/administración & dosificación , Administración Oral , Animales , Antiinflamatorios no Esteroideos/administración & dosificación , Inhibidores de la Ciclooxigenasa/administración & dosificación , Inhibidores de la Ciclooxigenasa/farmacología , Perros , Método Doble Ciego , Duodenoscopía/veterinaria , Duodeno/efectos de los fármacos , Gastroscopía/veterinaria , Lactonas/administración & dosificación , Inhibidores de la Lipooxigenasa/administración & dosificación , Inhibidores de la Lipooxigenasa/farmacología , Pirroles/administración & dosificación , Estómago/efectos de los fármacos , Sulfonas/administración & dosificación , Resultado del TratamientoRESUMEN
OBJECTIVE: To evaluate the effects of hydromorphone, hydromorphone and glycopyrrolate, medetomidine, and butorphanol premedication on the difficulty and time required to pass an endoscope into the stomach and duodenum of cats anesthetized with ketamine and isoflurane. DESIGN: Randomized complete block crossover study. ANIMALS: 8 purpose-bred adult female cats. PROCEDURES: Each cat was premedicated and anesthetized 4 times with an interval of at least 7 days between procedures. Cats were premedicated with hydromorphone, hydromorphone and glycopyrrolate, medetomidine, or butorphanol administered IM. Twenty minutes after premedication, sedation was assessed by use of a subjective ordinal scale. Cats received ketamine administered IM, and 10 minutes later a cuffed orotracheal tube was placed and anesthesia maintained with isoflurane. Cats breathed spontaneously throughout the procedure. When end-tidal isoflurane concentration was stable at 1.4% for 15 minutes, endoscopy was begun. The times required to pass the endoscope through the cardiac and pyloric sphincters were recorded, and the difficulty of endoscope passage was scored by use of a subjective ordinal scale. RESULTS: No significant differences in difficulty or time required to pass the endoscope through the cardiac and pyloric sphincters were found among premedicant groups. Premedication with medetomidine resulted in the greatest degree of sedation and longest time to return to sternal recumbency. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that hydromorphone, hydromorphone and glycopyrrolate, medetomidine, and butorphanol at the doses tested can be used satisfactorily to premedicate cats prior to general anesthesia for gastroduodenoscopy.
Asunto(s)
Analgésicos/farmacología , Gatos/fisiología , Parasimpatolíticos/farmacología , Medicación Preanestésica/veterinaria , Anestesia General/métodos , Anestesia General/veterinaria , Anestésicos Combinados , Animales , Butorfanol/farmacología , Estudios Cruzados , Duodenoscopía/métodos , Duodenoscopía/veterinaria , Femenino , Gastroscopía/métodos , Gastroscopía/veterinaria , Glicopirrolato/farmacología , Hidromorfona/farmacología , Isoflurano , Ketamina , Medetomidina/farmacología , Medicación Preanestésica/métodos , Distribución AleatoriaRESUMEN
OBJECTIVE: To evaluate the safety, with respect to the development of gastric ulcers and erosions, of concurrent administration of meloxicam and dexamethasone for 3 days to healthy dogs. ANIMALS: 20 conditioned purpose-bred research Beagles. PROCEDURE: Seven days prior to treatment, dogs were anesthetized for endoscopic evaluation of the upper portion of the gastrointestinal tract (ie, the gastric and duodenal mucosa). Five regions of the gastroduodenal area were scored by 2 investigators. Dogs were randomly assigned to 1 of 4 treatment groups as follows: saline-saline, dexamethasone-saline, saline-meloxicam, and dexamethasone-meloxicam groups. On days 1, 2, and 3, dogs received either dexamethasone or saline (0.9% NaCl) solution injections SC twice daily. On days 2, 3, and 4, dogs received either meloxicam or saline solution injections SC once daily. On day 2, dogs were anesthetized for a sham surgery (ie, electrostimulation). On day 5, the gastroduodenal area of each dog was reevaluated by use of endoscopic evaluation and histologic examination of biopsy specimens. RESULTS: The total endoscopic score of the dexamethasone-meloxicam group was significantly greater than the scores of the other groups. The dexamethasone-saline group had a mean cumulative score that was significantly greater than the saline-meloxicam or saline-saline groups. Endoscopic scores of the saline-meloxicam group were not significantly different from scores of the saline-saline group. No significant differences in histologic findings were found between groups. CONCLUSIONS AND CLINICAL RELEVANCE: In healthy dogs, meloxicam appears to be safe with regard to adverse effects on the gastrointestinal tract. Concurrent administration of dexamethasone and meloxicam is more likely to cause gastric erosions than meloxicam administration alone.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Dexametasona/farmacología , Mucosa Gástrica/fisiología , Mucosa Intestinal/fisiología , Tiazinas/farmacología , Tiazoles/farmacología , Animales , Antiinflamatorios/farmacología , Perros , Interacciones Farmacológicas , Duodenoscopía/veterinaria , Duodeno , Mucosa Gástrica/efectos de los fármacos , Gastroscopía/veterinaria , Mucosa Intestinal/efectos de los fármacos , Meloxicam , Valores de Referencia , Seguridad , EstómagoRESUMEN
Animal models have become an essential tool in the investigations of gut motility under experimental conditions. To determine the influence of various anaesthetic drugs on the motility pattern of the gastroduodenal tract, a new long-term model has had to be developed for allowing measurements in conscious and unrestrained as well as in sedated and analgosedated pigs. Since mechanical ventilation influences gut motility, it was necessary that this animal model enabled the investigation of the effect of drugs causing sedation and analgosedation during spontaneous breathing. Seven male, castrated pigs, German landrace, 32-40 kg bodyweight (BW) were investigated in this study. After habituation of the pigs to local housing conditions over 5 days, the animals were trained over 4 days to prepare for experimental situations and investigators. Pigs were inserted with a central venous catheter and with percutaneous enterogastrostomy (PEG) under general anaesthesia. Intestinal motility was measured by intraluminal impedancometry. The catheter was introduced over the PEG into the stomach and positioned into the duodenum by duodenoscopy. Measurements were done in conscious, unrestrained pigs and with sedated, and analgosedated animals on subsequent days. The habituation and training of the pigs to the investigators and for the laboratory conditions took between 7 and 9 days. The initial anaesthesia protocol for the instrumentation using remifentanil/propofol led to pyloric spasm and was thus unsuitable for duodenal intubation with an endoscope. In contrast, a combination of ketamine/propofol enabled this procedure. It was practicable to measure gut motility in conscious, unrestrained pigs. Spontaneous breathing was sufficient under propofol sedation and analgosedation using fentanyl-propofol. Systematically local application of polividon iodine in the area of the subcutaneous catheters avoided the necessity of using systemic prophylactic antibiotics. In conclusion, the habituation and training for 9 days enabled the measurement of gut motility by intraluminal impedancometry in conscious pigs. The insertion of the catheter was done during general anaesthesia using a combination of propofol and ketamine. For the future determination of gut motility performed under general anaesthesia, each sedation and analgosedation concept has to be evaluated to see whether it allows spontaneous breathing or whether mechanical ventilation is necessary.
Asunto(s)
Motilidad Gastrointestinal/fisiología , Habituación Psicofisiológica , Modelos Animales , Porcinos/fisiología , Animales , Duodenoscopía/veterinaria , Gastroscopía/veterinaria , Gastrostomía/veterinaria , MasculinoRESUMEN
Two dogs were emaciated and hypoalbuminemic due to protein-losing enteropathy associated with a severe, focal, mucoid, cryptal ectasia of the duodenum and marked villus atrophy. In one case, diseased portions of the duodenum were obvious endoscopically and were limited to discrete, focal areas in the small intestine, with apparently more undiseased tissue than diseased tissue being present. The signs and lesions in one dog resolved after initiating combination dietary and pharmacological therapy.
Asunto(s)
Enfermedades de los Perros/diagnóstico , Enfermedades Duodenales/veterinaria , Hipoalbuminemia/veterinaria , Enteropatías Perdedoras de Proteínas/veterinaria , Animales , Diagnóstico Diferencial , Dieta , Enfermedades de los Perros/patología , Enfermedades de los Perros/terapia , Perros , Enfermedades Duodenales/complicaciones , Enfermedades Duodenales/diagnóstico , Duodenoscopía/veterinaria , Femenino , Hipoalbuminemia/complicaciones , Hipoalbuminemia/diagnóstico , Inmunosupresores/uso terapéutico , Mucosa Intestinal/patología , Masculino , Enteropatías Perdedoras de Proteínas/complicaciones , Enteropatías Perdedoras de Proteínas/diagnósticoRESUMEN
Endoscopic examination of the upper gastrointestinal (GI) tract in dogs and cats is a relatively recent development. It was just over 20 years ago, in 1978, that the first series report of GI endoscopy in dogs and cats was presented. In those initial days, endoscopy was truly a novelty, and the primary uses for endoscopes were to retrieve foreign objects or visualize the esophagus and stomach. It was not until the mid-1980s that technology and training made gastroduodenoscopy standard practice. The evolution in endoscopic technology has also mirrored the evolution in computers and imaging modalities. The original fiberoptic endoscopes, although still available, are giving way to video endoscopy and digital imaging capabilities that provide spectacular images and an ability to generate hard copy images for medical records, teaching purposes, and research data that was previously not possible.