RESUMEN
OBJECTIVE: To evaluate clinical and laboratory outcomes of oocyte donation cycles and compare the results from donors and recipients. METHODS: A retrospective cohort study was conducted at a reproductive medicine center. A 586 first fresh oocyte donation cycles, performed from 01/2002 to 12/2017 were included. The outcomes of 290 cycles from donors and 296 from recipients, resulting in 473 fresh embryo transfers, were analyzed. The oocyte division was equally made, whereas, at an odd amount, the donor always had a preference. The data were collected from an electronic database, and analyzed using Chi-square test, Fisher's exact test, Mann-Whitney U-test or Student t-test depending on the data distribution, and multivariate logistic regression, considering p<0.05. RESULTS: The main results comparing donor and recipient, were, respectively: fertilization rate (72.0±21.4 vs. 74.6±24.2, p<0.001), implantation rate (46.2% vs. 48.5%, p=0.67); clinical pregnancy rate (41.9% vs. 37.7%, p=0.39), live birth rates by transfer (33.3 vs. 37.7, p=0.54). CONCLUSIONS: Oocyte donation is often the way donors can access in vitro fertilization, and for recipients seems to be a good option for pregnancy. Demographic and clinical characteristics have a secondary role in oocyte donors under 35 years and patient without comorbidities under 50 years and were not associated with pregnancy outcomes, emphasizing the power of oocyte quality on the success of intracytoplasmic sperm injection treatment. An oocyte-sharing program that offers good and comparable results is fair and worth being encouraged.
Asunto(s)
Donación de Oocito , Semen , Embarazo , Femenino , Masculino , Humanos , Estudios Retrospectivos , Donación de Oocito/métodos , Índice de Embarazo , Resultado del Embarazo , Fertilización In Vitro/métodos , OocitosRESUMEN
PURPOSE: To determine whether in vitro fertilization cycles using fresh oocyte donations benefit from preimplantation genetic testing for aneuploidies. METHODS: A paired cohort study compared 44 fresh oocyte donation cycles with or without preimplantation genetic testing for aneuploidy (PGT-A). The sibling oocyte study analyzed fertilized oocytes, blastocyst development, and euploidy rate. Only frozen embryo transfers were performed. Pregnancy, implantation, biochemical pregnancy, miscarriage, stillbirth, live birth, and twin pregnancy rates were analyzed between groups. RESULTS: Fresh oocyte donation cycles between PGT-A and non-PGT-A groups were similar in all laboratory and clinical outcomes. A euploidy rate of 74.2% was observed in the PGT-A group. Although a slight trend was observed for implantation rate in the PGT-A group, it was not statistically significant. No difference was observed for live birth between groups. CONCLUSION: PGT-A associated with fresh oocyte donation cycles does not improve clinical outcomes and can be seen as over-treatment for patients.
Asunto(s)
Aborto Espontáneo/epidemiología , Aneuploidia , Pruebas Genéticas/métodos , Nacimiento Vivo/epidemiología , Donación de Oocito/métodos , Oocitos/crecimiento & desarrollo , Diagnóstico Preimplantación/métodos , Adulto , Tasa de Natalidad , Brasil/epidemiología , Implantación del Embrión , Femenino , Fertilización In Vitro/métodos , Humanos , Masculino , Embarazo , Índice de Embarazo , Estudios Retrospectivos , Adulto JovenRESUMEN
OBJECTIVE: In order to help make the dream of parenthood come true for oocyte acceptors, it is essential that the procedure is not dangerous or unpleasant for oocyte donors. The aim of this study was to identify differences in safety, efficacy and patient acceptability between a traditional stimulation antagonist protocol with recombinant-FSH (rFSH) with hCG-triggering, compared with an innovative antagonist protocol with corifollitropin alfa (Elonva®) plus GnRH agonist triggering in oocyte donors. METHODS: A prospective longitudinal study was conducted at an in vitro fertilization center in Greece. The same eighty donors underwent two consecutive antagonist stimulation schemes. Primary outcomes were patient satisfaction (scored by a questionnaire) and delivery rate per donor. Secondary outcomes were mean number of cumulus-oocyte-complexes, metaphase II (MII) oocytes and ovarian hyperstimulation syndrome (OHSS) rate. RESULTS: Donors reported better adherence and less discomfort with the corifollitropin alpha + GnRH agonist-triggering protocol (p<0.001). No significant differences were identified in the clinical pregnancy rate per donor (p=0.13), the delivery rates, the number of oocytes (p=0.35), the number of MII oocytes (p=0.50) and the number of transferred embryos, between the two protocols. However, the luteal phase duration was significantly shorter (p<0.001) in the corifollitropin alpha + GnRH agonist-triggering protocol. Moreover, three cases of moderate OHSS (3.75%) were identified after hCG triggering, whereas no case of OHSS occurred after GnRH agonist ovulation induction (p=0.25). CONCLUSION: The use of corifollitropin alpha combined with a GnRH agonist for triggering is a safe, effective and acceptable protocol for oocyte donors.
Asunto(s)
Fármacos para la Fertilidad Femenina/administración & dosificación , Hormona Folículo Estimulante Humana/administración & dosificación , Hormona Liberadora de Gonadotropina/agonistas , Donación de Oocito/métodos , Oocitos/efectos de los fármacos , Inducción de la Ovulación/métodos , Adulto , Femenino , Fármacos para la Fertilidad Femenina/efectos adversos , Hormona Folículo Estimulante Humana/efectos adversos , Humanos , Estudios Longitudinales , Embarazo , Índice de Embarazo , Estudios Prospectivos , Adulto JovenRESUMEN
OBJECTIVE: Assisted reproduction combines innovative technologies and new forms of procreation through gamete donation; however, it also leads to moral and ethical issues and to the wide application of referential bioethics. The objective of the present study was to understand the bioethical context of shared oocyte donation. METHODS: The present qualitative study used the Collective Subject Discourse methodology to interview donors and recipients in Brazil. RESULTS: Donors suffer from infertility, and in vitro fertilization opens the possibility of having a child; however, the cost is high, and helping the recipient is more important than the financial cost. The recipients regret delaying motherhood; adopting a child is their last option, and they desire to feel the physical stages of pregnancy. The recipients find the rules unfair regarding the lack of an oocyte bank and the fact that the treatment must be performed in shared cycles; however, oocyte donation makes it possible to realize the common dream of motherhood. CONCLUSION: The obtained data showed that the patients are suffering and frustrated due to infertility, and they realize that in vitro fertilization may be the treatment they need. These women believe that children are essential in the constitution of the family, and scientific advances bring about innovative technologies and new forms of family constitution, with repercussions in the social, economic, political, and family contexts that lead to bioethical questions in Postmodernity.
OBJETIVO: A reprodução assistida agrega tecnologias inovadoras e novas formas de procriação por meio da doação de gametas; no entanto, também leva a questões éticas e morais e à ampla aplicação da bioética referencial. O objetivo deste estudo foi compreender o contexto bioético da doação compartilhada de oócitos. MéTODOS: Este estudo qualitativo utilizou a metodologia do Discurso do Sujeito Coletivo para entrevistar doadoras e receptoras no Brasil. RESULTADOS: As doadoras sofrem de infertilidade, e a fertilização in vitro abre a possibilidade de ter um filho; no entanto, o custo é alto, e ajudar a receptora é mais importante do que o custo financeiro. As receptoras se arrependem de retardar a maternidade; adotar uma criança é sua última opção, e elas desejam sentir os estágios físicos da gravidez. As receptoras consideram as regras injustas em relação à falta de um banco de oócitos e ao fato de que o tratamento deve ocorrer em ciclos compartilhados; no entanto, a doação de oócitos possibilita a realização do sonho comum da maternidade. CONCLUSãO: Os dados obtidos mostraram que as pacientes estão sofrendo e frustradas devido à infertilidade, e percebem que a fertilização in vitro pode ser o tratamento de que necessitam. Essas mulheres acreditam que as crianças são essenciais na constituição da família, e os avanços científicos agregam tecnologias inovadoras e novas formas de constituição familiar com repercussões nos contextos sociais, econômicos, políticos e familiares que levam a questões bioéticas na Pós-modernidade.
Asunto(s)
Actitud , Discusiones Bioéticas , Donación de Oocito/ética , Adulto , Brasil , Femenino , Humanos , Donación de Oocito/métodos , Investigación Cualitativa , AutoinformeRESUMEN
Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by in vitro embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.
Asunto(s)
Femenino , Animales , Lactante , Bovinos , Bovinos/embriología , Donación de Oocito/métodos , Laparoscopía , Laparoscopía/veterinaria , Embrión de Mamíferos/embriologíaRESUMEN
Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by in vitro embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.(AU)
Asunto(s)
Animales , Femenino , Lactante , Bovinos , Laparoscopía , Laparoscopía/veterinaria , Donación de Oocito/métodos , Bovinos/embriología , Embrión de Mamíferos/embriologíaRESUMEN
Abstract Objective Assisted reproduction combines innovative technologies and new forms of procreation through gamete donation; however, it also leads to moral and ethical issues and to the wide application of referential bioethics. The objective of the present study was to understand the bioethical context of shared oocyte donation. Methods The present qualitative study used the Collective Subject Discourse methodology to interview donors and recipients in Brazil. Results Donors suffer from infertility, and in vitro fertilization opens the possibility of having a child; however, the cost is high, and helping the recipient is more important than the financial cost. The recipients regret delaying motherhood; adopting a child is their last option, and they desire to feel the physical stages of pregnancy. The recipients find the rules unfair regarding the lack of an oocyte bank and the fact that the treatment must be performed in shared cycles; however, oocyte donation makes it possible to realize the common dream of motherhood. Conclusion The obtained data showed that the patients are suffering and frustrated due to infertility, and they realize that in vitro fertilization may be the treatment they need. These women believe that children are essential in the constitution of the family, and scientific advances bring about innovative technologies and new forms of family constitution, with repercussions in the social, economic, political, and family contexts that lead to bioethical questions in Postmodernity.
Resumo Objetivo A reprodução assistida agrega tecnologias inovadoras e novas formas de procriação pormeio da doação de gametas; no entanto, também leva a questões éticas e morais e à ampla aplicação da bioética referencial. O objetivo deste estudo foi compreender o contexto bioético da doação compartilhada de oócitos. Métodos Este estudo qualitativo utilizou a metodologia do Discurso do Sujeito Coletivo para entrevistar doadoras e receptoras no Brasil. Resultados As doadoras sofrem de infertilidade, e a fertilização in vitro abre a possibilidade de ter um filho; no entanto, o custo é alto, e ajudar a receptora é mais importante do que o custo financeiro. As receptoras se arrependem de retardar a maternidade; adotar uma criança é sua última opção, e elas desejam sentir os estágios físicos da gravidez. As receptoras consideramas regras injustas emrelação à falta de um banco de oócitos e ao fato de que o tratamento deve ocorrer emciclos compartilhados; no entanto, a doação de oócitos possibilita a realização do sonho comum da maternidade. Conclusão Os dados obtidosmostraram que as pacientes estão sofrendo e frustradas devido à infertilidade, e percebem que a fertilização in vitro pode ser o tratamento de que necessitam. Essas mulheres acreditam que as crianças são essenciais na constituição da família, e os avanços científicos agregam tecnologias inovadoras e novas formas de constituição familiar com repercussões nos contextos sociais, econômicos, políticos e familiares que levam a questões bioéticas na Pós-modernidade.
Asunto(s)
Humanos , Femenino , Adulto , Actitud , Donación de Oocito/ética , Discusiones Bioéticas , Brasil , Donación de Oocito/métodos , Investigación Cualitativa , AutoinformeRESUMEN
OBJECTIVE: Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS: A total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos). Fertilization rates, embryo quality at day 3, blastocysts development, and aneuploidy embryo rates were then compared. RESULTS: There was no difference in seminal parameters (volume, concentration and motility) in the studied groups. Fertilization rate, percentages of zygotes that underwent cleavage, and good-quality embryos on Day 3 were similar between the three groups evaluated. The group of men ≥50 years had significantly more sperm with damaged DNA, higher global aneuploidy rates, and significantly more embryos with trisomy 21, 18 or 13 compared to the other two evaluated groups (p<0.05). CONCLUSIONS: Our data shows that advanced paternal age increases global chromosomal abnormalities, and percentages of trisomy 21, 18 or 13 in embryos, and such effect is significantly important as of the age of 50. Embryo genetic screening is highly recommended in patients in which paternal age is ≥50 years old.
Asunto(s)
Envejecimiento/fisiología , Fertilización In Vitro/estadística & datos numéricos , Donación de Oocito/estadística & datos numéricos , Edad Paterna , Diagnóstico Preimplantación/estadística & datos numéricos , Trisomía , Adulto , Síndrome de Down/diagnóstico , Síndrome de Down/embriología , Síndrome de Down/epidemiología , Femenino , Pruebas Genéticas/estadística & datos numéricos , Humanos , Masculino , Persona de Mediana Edad , Donación de Oocito/métodos , Embarazo , Índice de Embarazo , Diagnóstico Preimplantación/métodos , Estudios Retrospectivos , Inyecciones de Esperma Intracitoplasmáticas/estadística & datos numéricos , Trisomía/diagnóstico , Trisomía/genética , Síndrome de la Trisomía 13/diagnóstico , Síndrome de la Trisomía 13/embriología , Síndrome de la Trisomía 13/epidemiología , Síndrome de la Trisomía 18/diagnóstico , Síndrome de la Trisomía 18/embriología , Síndrome de la Trisomía 18/epidemiologíaRESUMEN
OBJECTIVE: Preeclampsia (PE) occurs in 4.6% of pregnancies worldwide. The social phenomenon of increasing maternal age has raised the demand for donor oocytes. Egg donation has allowed women with poor ovarian reserve, premature ovarian failure, genetic disorders or surgical menopause to get pregnant. Recipients provide a unique model of immune response because of the differences in the genetic makeup of mothers and fetuses. In PE, immune tolerance may be impaired as a result of having non-autologous eggs implanted. Egg donation is a highly successful assisted reproductive technology, despite the significant number of issues arising from the implantation of non-autologous eggs. This study aimed to determine whether there is an association between egg donation and preeclampsia. METHODS: A systematic review of the literature available in PubMed and Google Scholar was carried out from January of 1995 to August of 2016 using the terms 'oocyte donation, preeclampsia', 'oocyte donation, in vitro fertilization, preeclampsia', 'oocyte donation, preeclampsia, outcomes pregnancies', 'oocyte donation, obstetric outcome.' Only six retrospective cohort studies met the selection criteria. RESULTS: The meta-analysis revealed a statistically significant association between egg donation and onset of preeclampsia (OR 4.50; 95% CI: 3.28-6.19; p<0.0001). CONCLUSION: Oocyte donation is associated with increased risk of preeclampsia in singleton pregnancies. Therefore, it is crucial to properly record and assess this finding when egg donation is the chosen assisted reproductive technology to attain pregnancy.
Asunto(s)
Fertilización In Vitro/efectos adversos , Donación de Oocito/efectos adversos , Preeclampsia/etiología , Adulto , Femenino , Fertilización In Vitro/métodos , Fertilización In Vitro/estadística & datos numéricos , Humanos , Donación de Oocito/métodos , Donación de Oocito/estadística & datos numéricos , Preeclampsia/diagnóstico , Preeclampsia/epidemiología , Embarazo , Resultado del Embarazo/epidemiología , Factores de RiesgoRESUMEN
OBJECTIVE: To compare the results obtained with two different GnRH agonist dosages: 0.3mg versus 0.4mg to trigger ovulation in oocyte donor cycles. METHODS: Experimental controlled randomized trial including 40 patients from a private practice center. The patients were randomized into two groups. Group A received a single dose of Triptorelin 0.3mg (Decapeptyl®) 36hours before pick-up. Group B patients received Triptorelin 0.4mg (Decapeptyl®) before pick-up to final oocyte maturation. We evaluated the total number of oocytes collected, the number of mature oocytes and total days of ovarian stimulation. RESULTS: The average of total collected oocytes were 16 (Group A) versus 15 (Group B), and the mean number of mature oocytes were 13 versus 12 respectively. The only variable showing a difference was the percentage of mature oocytes, which was greater in Group A, resulting in 84.6%, in contrast with those treated with 0.4mg of Triptorelin (78.6%), although these differences were not statistical significant (p=0.35). Days of stimulation did not differ between groups. No cases of empty follicle syndrome were reported. CONCLUSIONS: We found that an increase from 0.3 to 0.4mg of triptorelin in an oocyte donation program might not improve outcomes. Nevertheless, more studies might be necessary, not only in oocyte donors but in sterile women as well, to evaluate how GnRH agonist dosage could affect the results among other factors.
Asunto(s)
Hormona Liberadora de Gonadotropina/agonistas , Donación de Oocito , Oocitos/citología , Inducción de la Ovulación , Pamoato de Triptorelina , Adulto , Femenino , Humanos , Donación de Oocito/métodos , Donación de Oocito/estadística & datos numéricos , Inducción de la Ovulación/métodos , Inducción de la Ovulación/estadística & datos numéricos , Pamoato de Triptorelina/administración & dosificación , Pamoato de Triptorelina/uso terapéutico , Adulto JovenRESUMEN
The aim of the study was to evaluate the feasibility of pre-selection of high or low responder does prior to the superovulatory protocols. Twenty Saanen does received 800 IU of equine chorionic gonadotropin (eCG) at the end of long-term progestogen treatment. Fourteen days later, a second progestogen protocol associated with a multiple-dose follicle stimulation hormone (FSH) treatment (5 IU/kg of FSH, in six decreasing doses between days 4 to 6 of the protocol) was administered. Transrectal ultrasound was used to assess the follicular status at the beginning of superovulatory treatments, at the oestrous onset and on the seventh day of the oestrous cycle for counting corpora lutea (CL). A significant lower number of CL was obtained in eCG-treated in comparision with FSH-treated does (p < 0.05). A quartic regression was able to explain the relationship between the number of CL in response to both treatments (r(2) =0.50; p < 0.05). Seventy per cent (14 of 20) of does maintained the same ovulatory response (high or low) after treatments. The Kappa (κ = 0.40; p < 0.05) and Spearman (rs = 0.39; p = 0.08) coefficients were able to show a relationship between treatments. Regarding the follicular status, there is a significant relationship between the number of small follicles (r = 0.71; r(2) =0.47; p < 0.01) and total follicles (r = 0.60; p < 0.01) at eCG and first FSH dose with the number of CL. Moreover, it was found a negative relationship between the presence of large follicles and the number of CL in response to eCG treatment (r = -0.44; p < 0.05), but not from FSH (p > 0.05). In conclusion, the screening test with eCG has the potential to identify Saanen does that will better respond to the superovulatory protocol with FSH. In addition, it highlighted the importance of an ultrasound evaluation prior to the beginning of superovulatory treatments with FSH to characterize the follicular status and identify the potential donors of high ovulatory response in MOET programmes in goats.
Asunto(s)
Cabras/fisiología , Gonadotropinas Equinas/administración & dosificación , Donación de Oocito/veterinaria , Animales , Cuerpo Lúteo/anatomía & histología , Cuerpo Lúteo/efectos de los fármacos , Femenino , Hormona Folículo Estimulante/administración & dosificación , Donación de Oocito/métodos , Folículo Ovárico/diagnóstico por imagen , Folículo Ovárico/efectos de los fármacos , Inducción de la Ovulación/métodos , Inducción de la Ovulación/veterinaria , Progestinas/administración & dosificación , Superovulación/fisiología , Ultrasonografía/veterinariaRESUMEN
OBJECTIVE: The objective of this prospective randomised study was to evaluate if there is an improvement in clinical outcomes when assisted hatching (AH) is performed in embryos derived from vitrified/warmed oocytes in an ovum donation programme using egg cryobanking. STUDY DESIGN: Sixty oocyte recipients in a donation programme using egg cryobanking were randomly allocated to the assisted hatched (AH, n=30) or control group (n=30). The pregnancy and implantation rates were compared between the groups. RESULTS: A total of 288 vitrified oocytes were warmed for the 60 recipients. Of the 288 vitrified oocytes, 94.8% survived. All surviving oocytes were sperm injected, and 83.5% underwent fertilisation. There were 172 good-quality embryos selected for transfer. The total pregnancy rate was 40%. The pregnancy rate did not differ between the AH and control groups (43.3% and 33.3%, respectively, p=0.1967), but AH resulted in a higher implantation rate (31.6% vs. 18.4%, p=0.0206). CONCLUSION: Our study demonstrates the effectiveness of AH in embryos derived from warmed oocytes. Our results also suggest that oocyte cryopreservation can be considered as a tool for providing highly successful outcomes in an egg donor programme.
Asunto(s)
Criopreservación/métodos , Implantación del Embrión , Fertilización In Vitro/métodos , Donación de Oocito/métodos , Adulto , Transferencia de Embrión , Femenino , Humanos , Embarazo , Índice de Embarazo , Vitrificación , Zona Pelúcida/fisiologíaRESUMEN
BACKGROUND A successful oocyte vitrification program is important for women with various indications for assisted reproduction technology. The objective of this study was to report the outcome of vitrification of oocytes, obtained through an oocyte donation program, by evaluating the embryo development, pregnancy and implantation rates (IRs) after blastocyst transfer. METHODS A total of 1098 oocytes were obtained from 78 donors. There were 312 oocytes used in the study group (vitrified oocytes) and 786 used in the control group (fresh oocytes). There were 34 recipients who received blastocysts obtained from vitrified oocytes and 58 recipients who received blastocysts from fresh oocytes. The fertilization rate, cleavage rate, embryo quality, pregnancy rate (PR) and IR were compared between groups. RESULTS Vitrified oocytes showed a survival rate of 89.4%. There was no difference in the fertilization rate (76.1 and 87.5%), Day 2 cleavage rate (96.3 and 98.0%) or blastocyst formation rate (41.3 and 45.3%) for the study and control groups, respectively. PRs, IRs and miscarriages rates (MRs) were similar for the study group compared with the control group (PR: 61.8 versus 60.0%; IR: 43.9 versus 42.9%; MR: 9.5 versus 5.9%). CONCLUSIONS The developmental competence of embryos obtained from vitrified oocytes is not affected by the vitrification procedure, since they preserve the potential to be fertilized and to develop into high-quality blastocysts, similar to embryos from fresh oocytes. The successful clinical outcome indicates the use of this procedure for oocyte donation programs and for oocyte storage in general.
Asunto(s)
Transferencia de Embrión/métodos , Donación de Oocito/métodos , Oocitos/citología , Aborto Espontáneo , Adolescente , Adulto , Fase de Segmentación del Huevo , Criopreservación/métodos , Femenino , Fertilización In Vitro/métodos , Humanos , Embarazo , Índice de Embarazo , Estudios Prospectivos , VitrificaciónRESUMEN
PURPOSE: To evaluate which is the minimum number of oocytes to be allocated to each recipient in a shared egg donor program. METHODS: We analyzed 953 recipients that received at least 4 metaphase II (MII) oocytes in the period 2006-2008. We retrospectively divided the recipients according to the number of MII oocytes actually received. RESULTS: No statistically significant differences were found among the analyzed strata in clinical pregnancy rate (A:43.7%; B:45.6%; C:48.6%; D:45.5%; E:53%, P=NS) and miscarriage rate. However, the rate of top quality transferred embryos, and the embryo freezing rate were significantly higher among those recipients that received 7 or more mature eggs. CONCLUSIONS: After a large sample was analyzed, no significant differences in fresh embryo transfer outcome were encountered when a different number of oocytes was allocated. A minimum of 4 MII oocytes seems to achieve satisfactory pregnancy rates in our shared egg donor program.
Asunto(s)
Criopreservación , Donación de Oocito/métodos , Oocitos/citología , Índice de Embarazo , Adulto , Transferencia de Embrión , Femenino , Fertilización In Vitro , Humanos , Metafase , Persona de Mediana Edad , Embarazo , Resultado del Embarazo , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
O presente artigo aborda de forma resumida as bases gerais da biotécnica de Manipulação de Oócitos Inclusos em Folículos Ovarianos Pré-antrais (MOIFOPA) com ênfase nos estudos relativos ao desenvolvimento de um ovário artificial na espécie caprina. A importância do ovário artificial para a elucidação da foliculogênese inicial, testes de drogas sobre a dinâmica folicular e a sua potencial utililização para a produção in vitro de embriões em larga escala respeitando-se o bem-estar animal é destacada.
The present paper summarizes the basic aspects of the biotechnology of manipulation of oocytes enclosed in ovarian preantral follicles emphasizing the studies to develop an artificial ovary in goat species.. The importance of the artificial ovary for the understanding of early folliculogenesis, evaluation the effects of different drugs on follicular dynamics and its potential use for the in vitro production of embryos in large scale ensuring the animal welfare is highlighted.
Asunto(s)
Animales , Donación de Oocito/métodos , Donación de Oocito/veterinaria , Fertilización In Vitro/veterinariaRESUMEN
O presente artigo aborda de forma resumida as bases gerais da biotécnica de Manipulação de Oócitos Inclusos em Folículos Ovarianos Pré-antrais (MOIFOPA) com ênfase nos estudos relativos ao desenvolvimento de um ovário artificial na espécie caprina. A importância do ovário artificial para a elucidação da foliculogênese inicial, testes de drogas sobre a dinâmica folicular e a sua potencial utililização para a produção in vitro de embriões em larga escala respeitando-se o bem-estar animal é destacada. (AU)
The present paper summarizes the basic aspects of the biotechnology of manipulation of oocytes enclosed in ovarian preantral follicles emphasizing the studies to develop an artificial ovary in goat species.. The importance of the artificial ovary for the understanding of early folliculogenesis, evaluation the effects of different drugs on follicular dynamics and its potential use for the in vitro production of embryos in large scale ensuring the animal welfare is highlighted.(AU)
Asunto(s)
Animales , Fertilización In Vitro/veterinaria , Donación de Oocito/métodos , Donación de Oocito/veterinariaRESUMEN
Protocols that controlled follicular wave emergence and ovulation have had a great impact on the application of on-farm embryo transfer, as they permitted the initiation of superstimulatory treatments at a self-appointed time. However, the most commonly used approach for synchronization of follicular wave emergence involved estradiol, which cannot be used in many countries. Therefore, alternative treatments are required. Mechanical removal of the dominant follicle by ultrasound-guided follicle aspiration was effective, but required the use of specialized equipment and trained technical staff, which made it difficult to utilize in the field. Exogenous GnRH or pLH have also been used to induce ovulation of a dominant follicle, synchronizing follicular wave emergence, but their efficacy was dependent on the stage of the dominant follicle at treatment; thus, the emergence of the ensuing follicular wave may be too variable for superstimulation. An alternative approach could be initiating treatments at the time of emergence of the first follicular wave, but the need to synchronize ovulation may be a disadvantage in groups of donors at random stages of the estrous cycle. The final alternative may be to use FSH or eCG to initiate a new wave, without regard to the presence of a dominant follicle, followed by superstimulatory treatment at a predetermined time. All alternatives need to be thoroughly investigated in order to confirm their utility in the superstimulation of donor cows, regardless of the stage of the estrous cycle and without compromising ova/embryo production.
Asunto(s)
Bovinos/fisiología , Donación de Oocito/veterinaria , Inducción de la Ovulación/veterinaria , Superovulación/fisiología , Animales , Sincronización del Estro/métodos , Femenino , Hormona Folículo Estimulante/farmacología , Gonadotropinas Equinas/farmacología , Donación de Oocito/métodos , Inducción de la Ovulación/métodosRESUMEN
Sharing donated oocytes among several recipients is an effective practice. It lowers the cost, shortens their waiting time, and offers a satisfactory pregnancy rate.
Asunto(s)
Transferencia de Embrión , Donación de Oocito/métodos , Oocitos/trasplante , Pautas de la Práctica en Medicina , Asignación de Recursos/métodos , Donantes de Tejidos , Adulto , Argentina , Femenino , Humanos , TrasplanteRESUMEN
INTRODUCTION: GnRH agonists and antagonists are utilized for avoiding premature ovulation in assisted reproductive cycles, (ART) this retrospective study was designed to compare both treatments in controlled ovarian hyperstimulation (HOC) in oocyte donors. MATERIAL AND METHODS: Between Jan99 and Mar03, 141 oocyte donors underwent ART receiving either 0.25 mg daily of a GnRH antagonist (Cetrorelix) from day 6 of stimulation (51 patients) or a long protocol with a GnRH agonist (Leuprolide acetate) (90 patients.) FSHr alone or with HMG or LHr were employed for ovarian stimulation. hCG (Profasi, Serono) was administrated when more than three follicles above 18 mm in diameter were observed, oocyte retrieval was performed 34 hours later. Embryo transfer was performed 3-5 days later. RESULTS: Both groups were homogeneus for age (p=0.142), day 3 FSH (p=0.115), type and total dose of gonadotrophins utilized. There were no significant differences in follicles number (p=0.522), oestradiol levels on the day of hCG (p=0.310) and fertilization rates (p=0.177) The mean number of oocytes retrieved and metaphase II oocytes was significantly lower in GnRH agonist group, (12 vs. 13.9, p=0.05 and 8.6 vs 11; p=0.007) There was no statistical differences in pregnancy and implantation rates between agonist and antagonist groups (52.2% vs 60.8%, 15.1% vs 18.3%; p=0.327 and 0.652). CONCLUSIONS: The high number of metaphase oocytes and the high pregnancy rate observed in the oocyte donors provide evidence that GnRH antagonist does not impair ovarian response, embryo quality or pregnany rates. In oocyte donors cycles the GnRH antagonist is a valid alternative to GnRH agonist, providing the benefit of more flexibility in patient's scheduling.
Asunto(s)
Fármacos para la Fertilidad Femenina/administración & dosificación , Hormona Liberadora de Gonadotropina/análogos & derivados , Hormona Liberadora de Gonadotropina/administración & dosificación , Antagonistas de Hormonas/administración & dosificación , Leuprolida/administración & dosificación , Donación de Oocito/métodos , Inducción de la Ovulación/métodos , Adulto , Implantación del Embrión , Estradiol/sangre , Femenino , Fertilización/efectos de los fármacos , Fertilización/fisiología , Fertilización In Vitro , Humanos , Infertilidad/terapia , Síndrome de Hiperestimulación Ovárica/tratamiento farmacológico , Embarazo , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
This article discusses the so-called "new" reproductive technologies. The author analyzes and challenges this adjective, since for over two decades this group of medical techniques and experiments has been widely disseminated in the medical market. The media's coverage of test tube babies, and especially developments in intervention on human germ cells and embryos, help challenge the supposed permanent novelty of everything surrounding reproductive technologies and genetic interventions. Society is doubtless experiencing an open process in full innovation, but the social and symbolic effects on planning maternity, paternity, and filiation are still not well perceived or discussed. To illustrate such contradictions, the article focuses on the case of oocyte donation, highlighting the need for new perspectives in terms of social control over the dissemination of reproductive technologies.