RESUMEN

Probiotic viability, metabolite concentrations, physicochemical parameters, and volatile compounds were characterized in Gueuze beers formulated with probiotic lactic acid bacteria (LAB) and yeast. Additionally, the sensory profile of the beers and the resistance of the probiotics to digestion were determined. The use of 2 International Bitterness Units resulted in high concentrations of probiotic LAB but a decline in probiotic yeast as pH decreased. Secondary fermentation led to the consumption of maltose, citric acid, and malic acid, and the production of lactic and propionic acids. Carbonation and storage at 4 °C had minimal impact on probiotic viability. The addition of probiotic LAB resulted in a distinct aroma profile with improved sensory characteristics. Our results demonstrate that sour beers produced with probiotic LAB and a probiotic yeast, and fermented using a two-step fermentation process, exhibited optimal physicochemical parameters, discriminant volatile compound profiles, promising sensory characteristics, and high probiotic concentrations after digestion.

Asunto(s)

Cerveza , Fermentación , Probióticos , Gusto , Compuestos Orgánicos Volátiles , Cerveza/análisis , Cerveza/microbiología , Probióticos/metabolismo , Probióticos/análisis , Compuestos Orgánicos Volátiles/metabolismo , Compuestos Orgánicos Volátiles/química , Humanos , Digestión , Lactobacillales/metabolismo , Lactobacillales/crecimiento & desarrollo , Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/química , Viabilidad Microbiana

RESUMEN

Tannins are phenolic compounds that provide benefits to ruminants due to their protein-binding affinities and antioxidant properties. However, tannins may also have negative orosensorial and postingestive effects that decrease feed intake. This study explored how lambs trade off the ingestion of crude protein (CP) with the ingestion of potentially beneficial and toxic condensed and hydrolyzable tannins, and the ensuing impacts on diet digestibility, animal performance, and blood parameters. Thirty-two lambs were housed in individual pens for 8 wk and had access to 2 isoenergetic diets that varied in the concentration of CP (High-high in protein [HP] or Low-least preferred [LP]) and the presence of a mix of condensed and hydrolyzable tannins (4% DM). Animals were assigned to 4 treatment groups (N = 8 lambs/group) and received a simultaneous offer of: HP and LP (Control); HP and LP + tannins (HP - LP+); HP+ tannins and LP (HP + LP-); and both HP and LP with tannins (HP + LP+). All lambs preferred HP over LP and they avoided tannins in the diets (P < 0.001). Dry matter and CP intake were the lowest in HP + LP- (P < 0.0001), and DMD did not differ between Control and the other groups (P > 0.05), but it was greater for HP - LP + (P < 0.0001). CP digestibility was greater for groups without tannins in HP (P < 0.0001), but average daily gain (ADG) did not differ among treatments (P > 0.05). No differences between Control and HP + LP + were found in plasma antioxidant activity, total phenolic concentration, or haptoglobin concentration (P > 0.05). Intake of tannins was HP + LP+ > HP + LP- > HP - LP + (P < 0.0001), and fecal excretion of nitrogen (N) was HP + LP+ > HP - LP+ = HP + LP- > Control (P < 0.05). In addition, intake of tannins with both diets reduced blood urea nitrogen (BUN) concentration relative to Control (P < 0.05), thus suggesting a shift in the partitioning of N excretion from urine to feces. In summary, lambs prioritized the selection of HP over LP, regardless of the presence of a tannin extract in either or both diets. Nevertheless, lambs modulated their tannin consumption as a function of the specific diet where the tannin extract was added, with increasing levels of intake as tannins were present in just LP, then in just HP, and finally in both diets. Dietary tannins did not constrain ADG and resulted in a shift in the partitioning of N excretion from urine to feces. Such shifts have been found to result in reduced production of environmental pollutants such as ammonia, nitrous oxide, and nitrates.

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Tannins are plant chemicals that can provide benefits to ruminants due to their antioxidant activity and positive effects on nutrient uptake. However, tannins could also promote negative (i.e., toxic) effects on herbivores. This study explored how lambs build their diets when offered between 2 diets that varied in protein concentration (low or high), both with and without tannins (4%) and the ensuing consequences on diet digestibility, animal performance, and some blood parameters indicative of antioxidant activity and inflammation. All lambs preferred the high-over the low-protein diet, regardless of the inclusion of tannins in the diets. Digestibility of dry matter did not differ between lambs that had diet choices with or without tannins, and body weight gains were not different across all treatments. No differences were found among dietary treatment groups in blood antioxidant activity or indicators of inflammation. Tannin intake promoted a shift in the fate of nitrogen excretion from urine to feces, suggesting reductions in environmental pollutants such as ammonia, nitrous oxide, and nitrates. Thus, tannins did not promote negative impacts on animal performance with potential concomitant beneficial effects on the environment.

Asunto(s)

Alimentación Animal , Dieta , Proteínas en la Dieta , Taninos , Animales , Taninos/farmacología , Taninos/administración & dosificación , Taninos/metabolismo , Alimentación Animal/análisis , Dieta/veterinaria , Ovinos/fisiología , Proteínas en la Dieta/metabolismo , Digestión/efectos de los fármacos , Masculino , Fenómenos Fisiológicos Nutricionales de los Animales

RESUMEN

The objective of this study was to evaluate the intake and digestibility of nutrients, production and composition of milk, and water intake of lactating dairy cows fed standard diets in which ground corn (C) was replaced by up to 100% in the diet by cactus pear (CP). Eight Girolando cows (½ Gyr x ½ Holstein), 3rd calf multiparous, with 60 to 80 days of lactation and an average weight of 450.0 ± 30.0 kg, were randomly distributed in a double Latin square (4 × 4). Four experimental diets were evaluated: (100% C + 0% CP), (66.6% C + 33.3% CP), (33.3% C + 66.6% CP), and (0% C + 100% CP). There was a difference (P < 0.05) in the intake of CP and EE (kg.day- 1), with a quadratic effect and a linear reduction, respectively, with substitution. The maximum CP intake of 3.05 kg.day- 1 was found at 46.07% and EE intake fell by 3.81 g for each percentage unit of cactus pear added to the diet. There was a quadratic difference (P < 0.05) for DM intake in %BW and g.kg BW0,75, with maximum values of 3.97%BW and 186.8 g.kg BW0,75 with the substitution of 54.83 and 53.17%, respectively. There was a quadratic effect (P < 0.05) for the intake of NDF in %BW, with the maximum intake of 1.3% at the 74.69% substitution level. There was a linear reduction (P < 0.05) in the digestibility of DM, NDF, and TDN, and for the other nutrients there was no substitution effect (P > 0.05). There was no difference (P > 0.05) in milk production and composition. There was a linear reduction (P < 0.05) in water intake. Cactus pear can be used in a promising way to replace ground corn up to 100% in the diet of lactating dairy cows.

Asunto(s)

Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Dieta , Digestión , Ingestión de Líquidos , Lactancia , Leche , Zea mays , Animales , Bovinos/fisiología , Femenino , Lactancia/fisiología , Alimentación Animal/análisis , Leche/química , Dieta/veterinaria , Zea mays/química , Cactaceae/química , Opuntia/química , Ingestión de Alimentos , Distribución Aleatoria

RESUMEN

This study aimed to evaluate the effect of replacing Tifton 85 hay (TH) with Moringa hay (MH) on the intake, apparent digestibility, ingestive behaviour, rumen parameters, serum attributes, nitrogen balance, water balance, and urinary attributes of sheep. Thus, 5 rams, ½ Dorper + ½ Santa Inês, that were 12 ± 4 months of age with an initial body weight of 32.8 ± 2.6 kg were distributed in metabolic cages in a Latin square design. The experiment lasted 85 days and was divided into five 17-day periods. The animals received rations with increasing replacement (0, 100, 200, 300, or 400 g/kg of dry matter in the total diet) of TH for MH. The forage: concentrate ratio of the diets was 40:60. Replacing TH with MH reduced (P < 0.05) the intake of organic matter, crude protein, neutral detergent fibre (NDFap), and total digestible nutrients by sheep. The apparent digestibility of NDFap and the feeding and rumination efficiencies of NDFap were reduced (P < 0.05) when MH replaced TH in the animals' diet. This replacement did not influence (P > 0.05) serum urea, total protein, creatinine, or aspartate aminotransferase in sheep. Replacing TH with MH decreased water intake and excretion (P < 0.05). In addition, nitrogen excretion via urine, urinary creatinine, and nitrogen absorption decreased (P < 0.05) when TH was replaced with MH. However, there was an increase in the sheep's nitrogen retention and urine pH. We recommend replacing 30% TH with MH in the diet of lambs because it does not compromise nutrient metabolism.

Asunto(s)

Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Dieta , Digestión , Moringa oleifera , Animales , Alimentación Animal/análisis , Masculino , Dieta/veterinaria , Moringa oleifera/química , Oveja Doméstica/fisiología , Rumen/metabolismo , Metaboloma , Equilibrio Hidroelectrolítico , Nutrientes/metabolismo , Nitrógeno/metabolismo , Ovinos

RESUMEN

Polyphenolic compounds are common constituents of human and animal diets and undergo extensive metabolism by the gut microbiota before entering circulation. In order to compare the transformations of polyphenols from yerba mate, rosemary, and green tea extracts in the gastrointestinal tract, simulated gastrointestinal digestion coupled with colonic fermentation were used. For enhancing the comparative character of the investigation, colonic fermentation was performed with human, pig and rat intestinal microbiota. Chemical analysis was performed using a HPLC system coupled to a diode-array detector and mass spectrometer. Gastrointestinal digestion diminished the total amount of phenolics in the rosemary and green tea extracts by 27.5 and 59.2 %, respectively. These reductions occurred mainly at the expense of the major constituents of these extracts, namely rosmarinic acid (-45.7 %) and epigalocatechin gallate (-60.6 %). The yerba mate extract was practically not affected in terms of total phenolics, but several conversions and isomerizations occurred (e.g., 30 % of trans-3-O-caffeoylquinic acid was converted into the cis form). The polyphenolics of the yerba mate extract were also the least decomposed by the microbiota of all three species, especially in the case of the human one (-10.8 %). In contrast, the human microbiota transformed the polyphenolics of the rosemary and green extracts by 95.9 and 88.2 %, respectively. The yerba mate-extract had its contents in cis 3-O-caffeoylquinic acid diminished by 78 % by the human microbiota relative to the gastrointestinal digestion, but the content of 5-O-caffeoylquinic acid (also a chlorogenic acid), was increased by 22.2 %. The latter phenomenon did not occur with the rat and pig microbiota. The pronounced interspecies differences indicate the need for considerable caution when translating the results of experiments on the effects of polyphenolics performed in rats, or even pigs, to humans.

Asunto(s)

Colon , Depsidos , Digestión , Fermentación , Ilex paraguariensis , Extractos Vegetales , Polifenoles , Ácido Rosmarínico , Rosmarinus , Animales , Humanos , Extractos Vegetales/metabolismo , Rosmarinus/química , Ratas , Ilex paraguariensis/química , Porcinos , Depsidos/metabolismo , Depsidos/análisis , Polifenoles/metabolismo , Polifenoles/análisis , Colon/metabolismo , Colon/microbiología , Masculino , Cinamatos/metabolismo , Cinamatos/análisis , Microbioma Gastrointestinal , Té/química , Ácido Quínico/análogos & derivados , Ácido Quínico/metabolismo , Ácido Quínico/análisis , Catequina/análogos & derivados , Catequina/metabolismo , Catequina/análisis , Cromatografía Líquida de Alta Presión , Camellia sinensis/química

RESUMEN

Amidst increasing awareness of diet-health relationships, plant-derived bioactive peptides are recognized for their dual nutritional and health benefits. This study investigates bioactive peptides released after Alcalase hydrolysis of protein from chachafruto (Erythrina edulis), a nutrient-rich South American leguminous plant, focusing on their behavior during simulated gastrointestinal digestion. Evaluating their ability to scavenge radicals, mitigate oxidative stress, and influence immune response biomarkers, this study underscores the importance of understanding peptide interactions in digestion. The greatest contribution to the antioxidant activity was exerted by the low molecular weight peptides with ORAC values for the <3 kDa fraction of HES, GD-HES, and GID-HES of 0.74 ± 0.03, 0.72 ± 0.004, and 0.56 ± 0.01 (µmol TE/mg protein, respectively). GD-HES and GID-HES exhibited immunomodulatory effects, promoting the release of NO up to 18.52 and 8.58 µM, respectively. The findings of this study highlighted the potential of chachafruto bioactive peptides in functional foods and nutraceuticals, supporting human health through dietary interventions.

Asunto(s)

Antioxidantes , Digestión , Erythrina , Péptidos , Proteínas de Plantas , Hidrólisis , Proteínas de Plantas/metabolismo , Proteínas de Plantas/química , Péptidos/química , Péptidos/metabolismo , Erythrina/química , Antioxidantes/farmacología , Antioxidantes/química , Antioxidantes/metabolismo , Humanos , Subtilisinas/metabolismo , Subtilisinas/química , Estrés Oxidativo , Tracto Gastrointestinal/metabolismo

RESUMEN

The objective of the present study was to evaluate the substitution of soybean meals by biodiesel co-products on the performance and nutrient balance of lactating cows on pasture. Twelve dairy cows (7/8 Holstein-Gir) in early lactation were included in this feeding trial. These animals had an average daily milk yield of 21.1 kg and were distributed in a 4 × 4 triple Latin square experimental design. Protein supplements fed to these lactating cows contained peanut meal, cotton and sunflower as a complete replacement for soybean meal. Nutrient intake and digestibility, milk production, microbial protein synthesis, energy and protein balance were the parameters assessed in this study. Supplements containing different biodiesel co-products did not influence (P > 0.05) the level of forage intake, supplementation and nutrient by lactating cows, except for the total digestible nutrients (TDN) intake which was higher (P ≤ 0.05) in cows supplemented with cottonseed meal. The cotton meal also promoted higher digestibility of TDN (P = 0.001) and total carbohydrates (TCHO; P = 0.001) of the diet and, as a result, higher energy consumption (P = 0.01) and lower negative energy balance (P = 0.01). Cottonseed meal is a co-product of the biodiesel industry that has the ability to replace soybean meal as a source of protein in the supplement without affecting performance and promoting greater energy availability, demonstrating its ability to reduce the effects of negative energy balance in cows at the beginning of lactation kept on tropical pastures.

Asunto(s)

Alimentación Animal , Biocombustibles , Suplementos Dietéticos , Lactancia , Animales , Bovinos/fisiología , Femenino , Alimentación Animal/análisis , Suplementos Dietéticos/análisis , Biocombustibles/análisis , Dieta/veterinaria , Digestión/efectos de los fármacos , Glycine max/química , Fenómenos Fisiológicos Nutricionales de los Animales , Aceite de Semillas de Algodón/administración & dosificación , Leche/química

RESUMEN

Yeast and fibrolytic enzymes serve as additives incorporated into the nutrition of ruminants to regulate rumen fermentation and increase the digestibility of fiber, thereby enhancing the efficiency of rumen fermentation. Two experiments were conducted to assess the impact of five diets: a control diet without additives, diets with yeast (Saccharomyces cerevisiae) or exogenous fibrolytic enzymes (EFE), and diets with a blend of 0.7yeast + 0.3EFE or 0.7EFE + 0.3Yeast (based on recommended levels in g/kg of total DM). In the first experiment, 40 five-month-old Santa Ines lambs (mean weight 25.0 ± 1.3 kg) were distributed in a completely randomized design (5 treatments and 8 lambs) for 81 days to evaluate performance, ingestive behaviour, and serum metabolites. In the second experiment, 25 Santa Ines male lambs weighing 25.7 ± 4.1 kg were housed in metabolic cages, in a randomized design with 5 treatments and 5 lambs, evaluating digestibility, nitrogen balance, and rumen pH. EFE supplementation increased intakes of dry matter (DM), total digestible nutrients (TDN), and apNDF (mean of 38.1, 5.26, and 27%, respectively) compared to yeast or the 0.7yeast-0.3EFE blend. Feed conversion was most efficient (mean of 27.1%) in lambs fed Yeast, 0.7EFE + 0.3yeast, and the control diet. Lambs fed 0.7yeast + 0.3EFE spent less time eating (mean of 16.5%) and more time idling (mean of 10.75%), whereas EFE-fed lambs spent more time eating (mean of 19.73%), and 0.7EFE + 0.3yeast-fed lambs spent more time ruminating (mean of 20.14%). Control group lambs chewed and ruminated less (means of 24.64 and 17.21%, respectively) compared to other treatments. Lambs on the 0.7yeast + 0.3EFE blend had higher eating and rumination efficiency rates for DM and apNDF (mean of 19.11 and 17.95%, respectively) compared to other additive treatments or individual additives. They also exhibited lower (means 7.59 g/d) urinary N excretion, with improved N retention (mean 3185 g/d) compared to the control group. There were significant effects on serum albumin and cholesterol concentrations, with the 0.7yeast + 0.3EFE blend showing higher albumin (mean 4.08 g/dL) levels, while diets without additives and yeast-EFE blends had higher cholesterol (mean of 62.51 g/dL) concentrations. Including Saccharomyces cerevisiae yeast along with 0.7 yeast + 0.3 EFE blend is recommended when feeding similar lamb diets to those used herein because it improves the efficiency of intake, rumination of DM and NDF, and nitrogen utilization without affecting the lamb performance.

Asunto(s)

Alimentación Animal , Dieta , Suplementos Dietéticos , Digestión , Rumen , Saccharomyces cerevisiae , Animales , Alimentación Animal/análisis , Masculino , Digestión/efectos de los fármacos , Dieta/veterinaria , Suplementos Dietéticos/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Oveja Doméstica/fisiología , Fermentación , Distribución Aleatoria , Fibras de la Dieta/análisis , Fibras de la Dieta/administración & dosificación

RESUMEN

In this study, the effect of in vitro gastrointestinal digestion of phenolic compounds, the total phenolic content, and the antioxidant potential of stingless bee honey were investigated. Among the 33 phenolic compounds investigated, 25 were quantified, and only eight were not bioaccessible (p-aminobenzoic acid, sinapic acid, pinobanksin, isorhamnetin, quercetin-3-glucoside, syringaldehyde, coumarin, and coniferaldehyde). Benzoic acid was predominant in most undigested samples (21.3 to 2414 µg 100 g-1), but its bioaccessibility varied widely (2.5 to 534%). Rutin, a glycosylated flavonoid, was quantified in all samples and might have been deglycosylated during digestion, increasing the bioaccessibility of quercetin in a few samples. Overall, the concentration of phenolic compounds prior digestion and their bioaccessibility varied greatly among samples. Nevertheless, higher concentrations before digestion were not correlated to greater bioaccessibility. This study is the first to assess the in vitro bioaccessibility of phenolic compounds in SBH, providing novel insights into SBH research.

Asunto(s)

Digestión , Miel , Fenoles , Miel/análisis , Abejas/metabolismo , Abejas/química , Fenoles/metabolismo , Fenoles/química , Animales , Brasil , Antioxidantes/química , Antioxidantes/metabolismo , Modelos Biológicos , Humanos

RESUMEN

The study aims to assess the impact of oven-drying and decontamination on crude protein concentration and in vitro crude protein digestibility of yellow mealworms. Two kilograms of 12-wk-old mealworm larvae were subjected to freezing prior to the drying process. Approximately 1.5 kg of mealworm larvae were divided into 3 groups and exposed to oven-drying at temperatures of 50 °C for 36 h, 60 °C, and 70 °C for 24 h each. At intervals of 2 h, sets of 3 replicates were withdrawn to record water loss. Consistent weight stabilization was observed at 36 h for 50 °C (T50), 18 h for 60 °C (T60), and 14 h for 70 °C (T70). The remaining 0.5 kg of mealworm larvae was divided and dried under treatments T50, T60, and T70. Each treatment was then split into 2 portions, with one portion subjected to 90 °C for 15 min (denoted as T50-90, T60-90, T70-90) to eliminate microbial contamination. The 6 treatments were then used to determine concentrations of dry matter, crude ash, crude protein, pre-caecal protein digestibility, and dry matter residues after neutral detergent fiber, acid detergent fiber, and acid detergent lignin treatments. No interaction was observed between drying and decontamination treatments (P > 0.17). Pre-caecal crude protein digestibility increased with decreasing temperature (T50: 58% crude protein; T60: 51% crude protein; T70: 50% crude protein). Therefore, lower temperatures for longer times preserve crude protein digestibility. These findings are crucial for understanding how drying temperature and time impact protein bioavailability.

Asunto(s)

Digestión , Larva , Tenebrio , Animales , Tenebrio/química , Tenebrio/metabolismo , Larva/crecimiento & desarrollo , Desecación , Alimentación Animal/análisis , Descontaminación/métodos , Proteínas en la Dieta/metabolismo , Proteínas en la Dieta/análisis , Proteínas de Insectos/metabolismo , Calor

RESUMEN

Bioactive peptides derived from native proteins modulate physiological processes in the metabolic pathways. Given that multiple protocols in the literature mimic the digestion of dietary components, gathering studies that use such models directed at protein digestion processes is critical. This systematic review aimed to gather evidence that adopted adequate experimental models to simulate human protein digestion. The databases searched were PubMed, Web of Science, ScienceDirect, Embase, Virtual Health Library, and Scopus. A total of 1985 articles were found, resulting in 20 eligible in vitro studies. The Office of Health Assessment and Translation was used to evaluate methodological quality. Seven studies used plant-based protein sources, twelve used animal protein sources, and one used both. The duration of the oral phase varied, although 60% of the studies employed a protein digestion period of 120 min. Amylase, pepsin, and pancreatin enzymes were utilized in 40% of the studies, with pH levels of 7, 3, and 7, respectively, during the oral, gastric, and intestinal phases. The INFOGEST harmonized static model was adopted by 65% of the studies; INFOGEST is the most effective model for simulating gastrointestinal protein processes in humans and can be used to answer several research questions because it describes experimental conditions close to the human physiological situation.

Asunto(s)

Digestión , Tracto Gastrointestinal , Digestión/fisiología , Humanos , Tracto Gastrointestinal/metabolismo , Tracto Gastrointestinal/fisiología , Modelos Biológicos , Proteínas en la Dieta/metabolismo , Animales

RESUMEN

This study evaluated the digestibility of whole mango (Mangifera indica) meal (WMM) and determined the growth performance, intestinal enzyme activity, and metabolic and hematologic responses of tambaqui (Colossoma macropomum) juveniles fed diets containing different proportions of corn meal (CM) substitution by WMM. Fish fed with graded levels of WMM (0 (control), 80, 160, 240, and 320 g kg diet-1), replacing part of the dietary CM. The apparent digestibility coefficients of WMM were above 96%. Diets with WMM did not affect growth performance or intestinal enzyme activity. However, they showed a positive linear effect on plasma glucose, amino acids, and albumin levels and a negative linear effect on hepatic aspartate aminotransferase activity and hepatic glycogen, plasma cholesterol, and hemoglobin levels. Increased erythrocyte values and decreased plasma triglyceride levels were verified in fish fed 80 and 160 g WMM kg diet-1. In conclusion, the WMM may be a viable alternative to the tambaqui juveniles' diet, and WMM could replace up to 16% of CM without harming the growth and health of tambaqui juveniles.

Asunto(s)

Alimentación Animal , Mangifera , Zea mays , Animales , Alimentación Animal/análisis , Digestión/fisiología , Dieta/veterinaria , Fenómenos Fisiológicos Nutricionales de los Animales/fisiología , Characiformes/fisiología , Characiformes/crecimiento & desarrollo , Characiformes/sangre , Glucemia/análisis , Hígado/metabolismo

RESUMEN

Soybean molasses (SBMO) is a byproduct derived from the production of soy protein concentrate, obtained through solubilization in water and alcohol. The utilization of SBMO as an animal feed ingredient shows promising potential, primarily due to its low cost and as a potential energy concentrate. This study aimed to assess the intake, digestibility, ruminal parameters (pH and ruminal ammonia - NH3), nitrogen retention (NR) and microbial protein synthesis in grazing beef cattle supplemented with SBMO as a substitute for corn during the rainy season. Five Nellore (10-month-old) bulls with an average initial weight of 246 ± 11.2 kg were utilized in a 5 × 5 Latin square design. The animals were housed in five paddocks, each consisting of 0.34 ha of Marandu grass (Urochloa brizantha). Five isonitrogenous protein-energy supplements (300 g crude protein [CP]/kg supplement) were formulated, with SBMO replacing corn at varying levels (0, 0.25, 0.50, 0.75, or 1.00 g-1 g). The supplements were provided daily at a quantity of 2.0 kg-1 animal. The inclusion of SBMO at any level of corn substitution did not significantly affect the intake of pasture dry matter or total dry matter (P > 0.10). Likewise, the intake of CP and, consequently, the ruminal concentration of NH3 did not differ among the SBMO levels. Increasing the inclusion of SBMO did not have a significant impact on NR (P > 0.10), indicating that animals receiving supplements containing 100% SBMO as a substitute for corn may perform similarly to animals receiving supplements with 100% corn (0% SBMO). Soybean molasses represents a viable alternative energy source for grazing beef cattle during the rainy season and can entirely replace corn without adversely affecting animal nutritional performance.

Asunto(s)

Alimentación Animal , Suplementos Dietéticos , Digestión , Glycine max , Melaza , Rumen , Estaciones del Año , Animales , Bovinos/fisiología , Alimentación Animal/análisis , Melaza/análisis , Masculino , Glycine max/química , Suplementos Dietéticos/análisis , Rumen/metabolismo , Zea mays/química , Dieta/veterinaria , Fenómenos Fisiológicos Nutricionales de los Animales , Lluvia , Nitrógeno/metabolismo

RESUMEN

This study was conducted to determine the best combination of protein sources in diets for jundiá, based on growth, metabolism, and nutrient deposition. Five protein combinations were tested: casein + fish meal (control), casein + gelatin, casein + albumin, casein + albumin + fish meal, and albumin + fish meal, in diets containing 370 g Kg-1 of crude protein and 13.4 MJ Kg-1 of digestible energy. The fish (9.38 ± 0.12 g) were allocated in a water recirculation system at a density of 3.35 g L-1 per experimental unit and fed until apparent satiety for 40 days with the diets. The fish fed with the control diet had the highest final weight, specific growth rate, protein and feed efficiency ratio, protein retention, and best apparent feed conversion. On the other hand, fish that received casein + albumin and albumin + fish meal diets showed worse results in growth and body protein retention, low trypsin and chymotrypsin activity, and high intestinal amylase activity. Therefore, the combination referred to as control (casein + fish meal) conclusively provides the best rhythm for nutrient digestion and metabolism processes, enabling fish to reach greater growth and retention of body protein with low whole-fish fat content.

Asunto(s)

Alimentación Animal , Proteínas en la Dieta , Animales , Alimentación Animal/análisis , Proteínas en la Dieta/administración & dosificación , Proteínas en la Dieta/metabolismo , Caseínas/administración & dosificación , Caseínas/metabolismo , Digestión/fisiología , Digestión/efectos de los fármacos , Fenómenos Fisiológicos Nutricionales de los Animales

RESUMEN

Toxorhynchites mosquitoes have an exclusively phytophagous feeding habit as adults, which leads to significant differences in their morphophysiology compared with haematophagous mosquitoes. However, the molecular mechanisms of digestion in this mosquito are not well understood. In this study, RNA sequencing of the posterior midgut (PMG) of the mosquito Toxorhynchites theobaldi was undertaken, highlighting its significance in mosquito digestion. Subsequently, a comparison was made between the differential gene expression of the PMG and that of the anterior midgut. It was found that the most abundant proteases in the PMG were trypsin and chymotrypsin, and the level of gene expression for enzymes essential for digestion (such as serine protease, α-amylase and pancreatic triacylglycerol lipase) and innate immune response (including catalase, cecropin-A2 and superoxide dismutase) was like that of haematophagous mosquitoes. Peritrophin-1 was detected in the entire midgut, with an elevated expression level in the PMG. Based on our findings, it is hypothesized that a non-haematophagic habit might have been exhibited by the ancestor of Tx. theobaldi, and this trait may have been retained. This study represents a pioneering investigation at the molecular level of midgut contents in a non-haematophagous mosquito. The findings offer valuable insights into the evolutionary aspects of feeding habits in culicids.

Asunto(s)

Culicidae , Animales , Culicidae/fisiología , Culicidae/metabolismo , Proteínas de Insectos/metabolismo , Proteínas de Insectos/genética , Transcriptoma , Perfilación de la Expresión Génica , Sistema Digestivo/metabolismo , Digestión , Tracto Gastrointestinal/metabolismo , Filogenia , Conducta Alimentaria

RESUMEN

The aim of this study was to explore the effect of replacing protein pellets with soybean grain in high-concentrate diets with or without the addition of silage, on the intake, digestibility, and rumen and blood parameters of feedlot cattle in tropical regions. Four cannulated, crossbred steers were used, 4.5 ± 0.5 years old, with an average weight of 685.55 ± 111.78 kg. The steers were distributed in a 4 × 4 Latin square, in a 2 × 2 factorial scheme (two sources of protein: protein pellets or whole soybean grain, with or without added dietary bulk). There was no effect (P ≥ 0.109) from the interaction between the source of protein and the addition of silage to the diet on dry matter (DM) and nutrient intake, or the digestibility (P ≥ 0.625) of DM or crude protein (CP). However, both factors affected (P ≤ 0.052) the intake of DM, neutral detergent fiber (NDF), and non-fiber carbohydrates (NFC), as well as the independent digestibility (P ≤ 0.099) of fat, NFC, total carbohydrates (TC), and total cholesterol concentration. There was an effect (P ≤ 0.053) from the interaction between the source of protein and the addition of silage to the diet on the digestibility of NDF and total digestible nutrients (TDN), as well as on the glycose concentration (P = 0.003). Blood parameters (i.e. protein, albumin, creatinine, triglycerides, aspartate aminotransferase (AST), and alanine aminotransferase (ALT)) were not affected (P ≥ 0.139) by the source of protein, the addition of silage, or their interaction. Lastly, including 150 g/kg silage DM in a high-grain diet, and using soybean grain as a source of protein in substitution of protein pellet could be a suitable nutritional strategy to ensure adequate DM and nutrient intake and digestibility, with no detrimental effects on rumen and blood parameters of feedlot cattle in the tropics.

Asunto(s)

Alimentación Animal , Fenómenos Fisiológicos Nutricionales de los Animales , Dieta , Digestión , Glycine max , Rumen , Clima Tropical , Animales , Bovinos/sangre , Bovinos/fisiología , Bovinos/metabolismo , Rumen/metabolismo , Masculino , Alimentación Animal/análisis , Digestión/fisiología , Dieta/veterinaria , Ensilaje/análisis , Proteínas en la Dieta/metabolismo , Proteínas en la Dieta/administración & dosificación , Nutrientes/metabolismo

RESUMEN

Live food in the initial life stages of neotropical fish is essential for their development and health; however, it can significantly increase production costs. This study uses complete diets with varying moisture contents (47%, 35%, 24%, and 8%) as a cost-effective alternative in the co-feeding phase of surubim larvae, assessing their influence on development, digestive enzymes, and liver metabolism. In a completely randomized design, 3200 three-day-old Pseudoplatystoma sp. larvae (0.001 g) were distributed evenly among 16 aquariums (20 L), with 200 individuals per aquarium. For the first five days, all larvae were fed Artemia exclusively, after which they were fed experimental diets with varying levels of humidity (47%, 35%, 24%, and 8%) six times a day across four treatments and four replicates. The 21-day feeding trial demonstrated that larvae fed with 24% and 8% moisture diets exhibited increased (p < 0.05) weight gain, final length, and protein efficiency rate. The remaining growth parameters (i.e., specific growth rate and condition factor) did not show significant differences (p > 0.05) among the dietary treatments. Enzymatic analysis revealed that the 47% moisture diet enhanced the amylase and alkaline phosphatase activities, whereas the 24% and 35% moisture diets elevated the lipase and protease activities. The 47% moisture diet also resulted in increased alanine aminotransferase, aspartate aminotransferase, and albumin levels, along with visible hepatic histopathologies in samples, such as visible lipid vacuoles, displacement of the nucleus of the hepatocyte, and increased sinusoid spaces. No significant liver changes were observed in fish fed with other diets. Principal component analysis showed that diets with 8-24% moisture content were the most beneficial during the co-feeding phase of surubim larviculture.

Asunto(s)

Alimentación Animal , Dieta , Larva , Animales , Alimentación Animal/análisis , Dieta/veterinaria , Larva/crecimiento & desarrollo , Bagres/crecimiento & desarrollo , Bagres/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Agua/metabolismo , Digestión/fisiología , Hígado/enzimología , Hígado/metabolismo

RESUMEN

The objective of this study was to evaluate the effects of amino resin-treated soybean meal (SBM) on ruminal fermentation, nutrient digestion, and N partitioning. The treatments were (1) untreated solvent-extracted SBM, (2) amino resin-treated SBM (AR-SBM), and (3) heat-treated SBM (HT-SBM). The experimental design was arranged as a replicated 3 × 3 Latin square with 6 fermentors in a dual-flow continuous culture system. Treatments were randomly assigned to fermentors within a Latin square for each period. Each fermentor was fed 106 g/d of diet DM equally distributed in 2 feeding times daily at 0800 and 1800 h. Diets were formulated to contain 16% CP, 30% NDF, and 30% starch across treatments. The experiment consisted of 3 experimental periods, each lasting for 10 d. The first 7 d of each period were considered adaptation, and the last 3 d were used for sampling and data collection. On d 8 and 9, samples were collected for analysis of diurnal variation in concentrations of NH3-N, pH, and VFA during the first 8 h after feeding. On d 8, 9, and 10, samples were collected from the liquid and solid effluents accumulated over 24 h for analysis of daily averages of NH3-N and VFA pools, and true ruminal digestibility estimates. Data were analyzed using the MIXED procedure of SAS, and significance was declared when P ≤ 0.05. The model included the fixed effect of treatment and random effects of square, period, and fermentor within square, whereas time and interaction treatment × time were included for analyses of diurnal variation, with time as repeated measures. Compared with SBM, the cultured ruminal contents of AR-SBM and HT-SBM had lower NH3-N concentrations, indicating lower microbial fermentation of protein. Molar proportions of isovalerate and isobutyrate were greater in SBM than AR-SBM and HT-SBM, with greater molar proportion of isobutyrate for SBM, particularly during the first 2 h after feeding. The flow of NH3-N was greater for SBM compared with AR-SBM and HT-SBM, whereas NAN flow, bacterial N flow, and N efficiency were greater for AR-SBM and HT-SBM compared with SBM. Our results indicate that both the amino resin and heat treatments of SBM allow for similar decreases in microbial degradation of CP without limiting microbial protein synthesis in diets with 16% CP. Amino resin treatment may be effective in reducing microbial fermentation of protein in the rumen without adverse effects on digestibility or fermentation parameters as compared with SBM.

Asunto(s)

Alimentación Animal , Dieta , Digestión , Fermentación , Glycine max , Nitrógeno , Rumen , Animales , Rumen/metabolismo , Nitrógeno/metabolismo , Dieta/veterinaria , Bovinos , Nutrientes/metabolismo

RESUMEN

BACKGROUND: This study evaluated for the first time the potential of orange passion fruit as a base for alcoholic and acetic fermentations, with a view to assessing its profile of organic acids and polyphenols, in vitro digestion, and biological activities. RESULTS: In terms of aliphatic organic acids, malic acid was the majority in the wine (3.19 g L-1), while in the vinegar, it was acetic acid (46.84 g L-1). 3,4-Dihydroxybenzoic acid (3,4-DHB) was the major phenolic compound in the wine and vinegar samples (3443.93 and 2980.00 µg L-1, respectively). After the in vitro gastrointestinal simulation stage, the wine showed high bioaccessibility for the compounds sinipaldehyde (82.97%) and 2,4-dihydroxybenzoic acid (2,4-DHBA, 81.27%), while the vinegar exhibited high bioaccessibility for sinipaldehyde (89.39%). Through multivariate analysis, it was observed that 3,4-DHB was highly concentrated in the different digested fractions obtained from the wine. In contrast, in the vinegar, the stability of isorahmenetin and Quercetin 3-o-rhamnoside was observed during the in vitro digestion simulation. Lastly, the vinegar stood out for its inhibition rates of α-amylase (23.93%), α-glucoside (18.34%), and angiotensin-converting enzyme (10.92%). In addition, the vinegar had an inhibitory effect on the pathogenic microorganisms Salmonella enteritidis, Escherichia coli, and Listeria monocytogenes. CONCLUSION: Orange passion fruit has proved to be a promising raw material for the development of fermented beverages. Therefore, this study provides an unprecedented perspective on the use and valorization of orange passion fruit, contributing significantly to the advancement of knowledge about fermented products and the associated nutritional and functional possibilities. © 2024 Society of Chemical Industry.

Asunto(s)

Ácido Acético , Digestión , Fermentación , Frutas , Passiflora , Fenoles , Vino , Passiflora/química , Passiflora/metabolismo , Frutas/química , Frutas/metabolismo , Ácido Acético/metabolismo , Ácido Acético/química , Ácido Acético/análisis , Fenoles/metabolismo , Fenoles/análisis , Fenoles/química , Vino/análisis , Humanos , Escherichia coli/efectos de los fármacos , Listeria monocytogenes/efectos de los fármacos , Malatos/análisis , Malatos/metabolismo , Extractos Vegetales/química , Extractos Vegetales/metabolismo , Extractos Vegetales/farmacología , Polifenoles/metabolismo , Polifenoles/análisis , Polifenoles/química

RESUMEN

The research aimed to assess the effects of incorporating germinated Lupinus angustifolius flour into corn extrudates for different periods (3, 5, and 7 days), focusing on starch digestibility, morphological structure, thermal, and pasting properties. Extrudate with germinated lupinus flour for 7 days (EG7) significantly increased the content of slowly digestible starch up to 10.56% (p < 0.05). Crystallinity increased up to 20% in extrudates with germinated flour compared to extrudates with ungerminated flour (EUG), observing changes at the molecular level by FTIR that impact the thermal and pasting properties. X-ray diffraction revealed angles of 2θ = 11.31, 16.60, 19.91, and 33.04 as a result of the germination and extrusion processes. Microstructural analysis indicated starch-protein interactions influencing changes in calorimetry, viscosity, X-ray diffraction, and digestibility. PCA allowed establishing that the addition of germinated flours significantly affected the properties and microstructural characteristics of extruded products, potentially affecting digestibility and nutritional quality.

Asunto(s)

Digestión , Germinación , Lupinus , Almidón , Difracción de Rayos X , Zea mays , Zea mays/química , Zea mays/crecimiento & desarrollo , Zea mays/metabolismo , Lupinus/química , Lupinus/metabolismo , Lupinus/crecimiento & desarrollo , Almidón/química , Almidón/metabolismo , Harina/análisis , Viscosidad , Semillas/química , Semillas/crecimiento & desarrollo , Semillas/metabolismo , Manipulación de Alimentos