RESUMEN
STATEMENT OF PROBLEM: Contrasting results have been reported regarding the influence of the use of adhesive on biofilm formation. PURPOSE: The purpose of this study was to evaluate the influence of the use of adhesive on the formation of biofilm on the internal surface of complete dentures and the palatal mucosa of denture wearers. MATERIAL AND METHODS: Thirty participants with well-fitting complete dentures were randomly divided according to the experimental design: protocol 1, adhesive use during the first 15 days, followed by no use of adhesive over the next 15 days; protocol 2, no use of adhesives during the first 15 days, followed by adhesive use over the next 15 days. After each period, material from the mucosa and intaglio of the maxillary dentures was collected. Replicate aliquots were plated onto Petri dishes containing selective media for Candida spp, Streptococcus mutans, and a nonselective culture medium. Colony-forming units were expressed as log (CFU+1)/mL. In addition, the internal surfaces of the maxillary and mandibular complete dentures were stained and photographed. From the photographs, the total internal surface and the surface stained with biofilm were quantified (software ImageTool 3.00), and the percentage of the biofilm-covered area (%) on the maxillary and mandibular dentures was calculated and compared with 2-way ANOVA. For the nonselective culture medium, data were compared with the paired-sample t test, and the Wilcoxon signed rank test was performed to compare the colony counts of Candida spp and Streptococcus mutans (α=.05). RESULTS: Similar colony counts were found with or without the use of adhesive for the mucosa and internal surfaces of maxillary dentures, irrespective of the culture medium (P>.05). The area of dentures covered with biofilm was influenced by the use of adhesive (P=.025), regardless of the type of denture (P=.121). CONCLUSIONS: The use of adhesive did not alter the colony counts of microorganisms from the palatal mucosa and maxillary dentures of complete denture wearers during the 15-day period, but it did influence the area covered with biofilm on the internal surfaces of the complete dentures.
Asunto(s)
Adhesivos/química , Biopelículas/crecimiento & desarrollo , Bases para Dentadura/microbiología , Retención de Dentadura , Hueso Paladar/microbiología , Adhesivos/economía , Anciano , Carga Bacteriana , Candida/aislamiento & purificación , Recuento de Colonia Microbiana , Colorantes , Estudios Cruzados , Medios de Cultivo , Dentadura Completa Inferior/microbiología , Dentadura Completa Superior/microbiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Rojo Neutro , Fotograbar , Streptococcus mutans/aislamiento & purificación , Propiedades de Superficie , Factores de TiempoRESUMEN
This study assessed how biofilm composition is affected by both time and denture material in complete denture wearers. Biofilm was formed during two phases of 14 days on acrylic resin and denture liner specimens mounted on the buccal surface of the mandibular dentures of 21 patients. Specimens were removed randomly on days 2, 7, and 14. Higher counts of Candida glabrata, total streptococci, Actinomyces, total microorganisms, and percentage of Actinomyces were observed after 7 and 14 days (P < .05). C glabrata was the only species to show progressively rising counts from day 2 to 14, while no difference was found in biofilm composition among the materials tested.
Asunto(s)
Biopelículas/clasificación , Materiales Dentales/química , Placa Dental/microbiología , Dentadura Completa Inferior/microbiología , Resinas Acrílicas/química , Actinomyces/aislamiento & purificación , Anciano , Candida/clasificación , Candida albicans/aislamiento & purificación , Candida glabrata/aislamiento & purificación , Candida tropicalis/aislamiento & purificación , Recuento de Colonia Microbiana , Estudios Cruzados , Alineadores Dentales/microbiología , Femenino , Estudios de Seguimiento , Humanos , Masculino , Streptococcus/clasificación , Streptococcus/aislamiento & purificación , Streptococcus mutans/aislamiento & purificaciónRESUMEN
OBJECTIVES: The objective of this study was to compare and correlate biofilm levels in complete upper and lower prosthesis after brushing, associated with specific paste and soap, by means of computerised methodology. MATERIALS AND METHODS: Forty-five complete denture wearers were selected and instructed to brush their prostheses (Soft Oral B 40) three times a day for 3 weeks with water (Control), specific paste for complete dentures (Corega Brite) (Experiment 1) and neutral soap (Experiment 2). The study was based on a cross-over model and a wash-out period was not included. For biofilm quantification, the internal surfaces were dyed (neutral red 1%), photographed (Canon EOS Digital) and the disclosed biofilm was measured with the Image Tool 2.0 software. The products were assessed by means of a questionnaire regarding their hygiene properties and acceptance. RESULTS: The variance analysis indicated that the lower prostheses exhibited a mean biofilm percentage, significantly higher than the upper prostheses and that brushing with paste (Experiment 1) was more effective than soap (Experiment 2) and, in turn, this was more effective than water (Control). There was a high biofilm correlation (Pearson correlation) between both prostheses. Both products were well accepted by the patients, but the most favoured one was the paste. CONCLUSIONS: This was effective in controlling the biofilm and can be used preventatively in the maintenance of oral health by wearers of complete dentures. This is important where the lower prosthesis can harbour microorganisms which may act as a reservoir for other areas of the mouth and thus enhance the importance of proper hygiene.