RESUMEN
High quantities of bacteriophages are currently used in the food industry and agriculture. However, growing antibiotic resistance of bacteria has recently awakened the interest to use bacteriophages for the treatment of bacterial infections in humans indicating that even higher quantities will be required in the future. High demand combined with a wide range of applications requires also efficient bacteriophage production processes operating at low production costs and with high productivity. To achieve this goal, different approaches were introduced and extensive studies of various parameters affecting bacteriophage formation were investigated. In this mini-review, we provide a short overview about different operation modes of bacteriophage production such as batch, semi-continuous and especially continuous with the pros and cons of each. We present factors affecting bacterial physiological state, its effect on phage formation and provide a description of methods for determination of bacteriophage growth parameters, through which bacteriophage formation is obtained. Understanding of described phenomena and inclusion of potential occurrence of mutations and selection in continuous systems enables evaluation of continuous process productivity and its optimization.
Asunto(s)
Bacterias/virología , Bacteriófagos/crecimiento & desarrollo , Bacteriófagos/aislamiento & purificación , Biotecnología/métodos , Cultivo de Virus/métodos , Bacterias/crecimiento & desarrollo , Biotecnología/economía , Humanos , Cultivo de Virus/economíaRESUMEN
Adenoviral infections are a major cause of morbidity and mortality after allogeneic hematopoietic stem cell transplantation (HSCT) in pediatric patients. Adoptive transfer of donor-derived human adenovirus (HAdV)-specific T-cells represents a promising treatment option. However, the difficulty in identifying and selecting rare HAdV-specific T-cells, and the short time span between patients at high risk for invasive infection and viremia are major limitations. We therefore developed an IL-15-driven 6 to 12 day short-term protocol for in vitro detection of HAdV-specific T cells, as revealed by known MHC class I multimers and a newly identified adenoviral CD8 T-cell epitope derived from the E1A protein for the frequent HLA-type A*02â¶01 and IFN-γ. Using this novel and improved diagnostic approach we observed a correlation between adenoviral load and reconstitution of CD8(+) and CD4(+) HAdV-specific T-cells including central memory cells in HSCT-patients. Adaption of the 12-day protocol to good manufacturing practice conditions resulted in a 2.6-log (mean) expansion of HAdV-specific T-cells displaying high cytolytic activity (4-fold) compared to controls and low or absent alloreactivity. Similar protocols successfully identified and rapidly expanded CMV-, EBV-, and BKV-specific T-cells. Our approach provides a powerful clinical-grade convertible tool for rapid and cost-effective detection and enrichment of multiple virus-specific T-cells that may facilitate broad clinical application.
Asunto(s)
Adenoviridae/inmunología , Linfocitos T CD4-Positivos/virología , Linfocitos T CD8-positivos/virología , Infecciones por Adenoviridae/inmunología , Infecciones por Adenoviridae/virología , Antígenos Virales/inmunología , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/inmunología , Técnicas de Cultivo de Célula/economía , Proliferación Celular , Células Cultivadas , Niño , Preescolar , Citotoxicidad Inmunológica , Femenino , Trasplante de Células Madre Hematopoyéticas/efectos adversos , Humanos , Masculino , Fenotipo , Trasplante Homólogo , Cultivo de Virus/economía , Adulto JovenRESUMEN
During certain months of the year, viral respiratory infections lead to a dramatic increase in pediatric emergency room visits and hospital admissions. Rapid identification of the infectious organism results in timely treatment and reductions in hospital cost and length of stay. Before the introduction of molecular testing to the virology laboratory, diagnosis relied on the standard methods of immunofluorescence and culture. These tests can be labor-intensive and costly. Recent studies have demonstrated the higher sensitivity, faster turnaround, and broader diagnostic spectrum provided by multiplexed RT-PCR assays. Data comparing the laboratory cost and labor efficiency of the tests are lacking. To address this issue, we chose to implement the principles of operational workflow analysis using lean methodology to critically evaluate the potential advantages of a multiplexed RT-PCR assay both in terms of workflow and cost effectiveness. Our results indicated that the implementation of the Luminex xTAG Respiratory Viral Panel (RVP) resulted in a standardized workflow with decreased requirements in laboratory cost as well as improvement in efficiency. In summary, we demonstrate that, in our laboratory, the Luminex xTAG RVP is more operationally streamlined and cost-effective than standard viral direct fluorescent antibody and culture. Further studies are needed to highlight additional benefits of the test, including shortened hospital stay and improved patient outcome.
Asunto(s)
Laboratorios/economía , Infecciones del Sistema Respiratorio/diagnóstico , Infecciones del Sistema Respiratorio/virología , Cultivo de Virus/métodos , Flujo de Trabajo , Antígenos Virales/análisis , Análisis Costo-Beneficio , Humanos , Laboratorios/normas , Cultivo de Virus/economíaAsunto(s)
Industria Farmacéutica/economía , Huevos/virología , Vacunas/economía , Cultivo de Virus/métodos , Animales , Investigación Biomédica/economía , Investigación Biomédica/tendencias , Industria Farmacéutica/tendencias , Estados Unidos , Vacunas/provisión & distribución , Cultivo de Virus/economía , Cultivo de Virus/tendenciasRESUMEN
The integrated cell culture quantitative reverse transcriptase PCR (ICC/qRT-PCR) method is used in our lab to detect enteroviruses in environmental waters. Typically we utilize monolayers of 3 cell lines; buffalo green monkey kidney (BGM), human colonic carcinoma (CACO-2) and African rhesus monkey kidney (MA104) with the intent of providing one or more permissive hosts to a wide range of enteroviruses. In this study the BGM cell line was used to compare poliovirus infectivity in conventional monolayer cultures to BGM cells in suspensions. Propagated virus was subsequently amplified by qRT-PCR. Our PCR data showed lower cycle threshold (Ct) values in the suspensions which corresponded to a higher rate of infectivity than that observed in the monolayers. The difference in Ct values was determined statistically significant by One-way ANOVA (0.000). Infecting BGM cells in suspensions required less hands-on time, less chance of contamination and was more cost effective than utilizing the conventional monolayer technique.
Asunto(s)
Poliovirus/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Virología/métodos , Microbiología del Agua , Animales , Técnicas de Cultivo de Célula/economía , Técnicas de Cultivo de Célula/métodos , Células Cultivadas , Chlorocebus aethiops , Humanos , Macaca mulatta , Poliovirus/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/economía , Sensibilidad y Especificidad , Virología/economía , Cultivo de Virus/economía , Cultivo de Virus/métodosRESUMEN
Effective laboratory methods for identifying avian influenza virus (AIV) in wild bird populations are crucial to understanding the ecology of this pathogen. The standard method has been AIV isolation in chorioallantoic sac (CAS) of specific-pathogen-free embryonating chicken eggs (ECE), but in one study, combined use of yolk-sac (YS) and chorioallantoic membrane inoculation routes increased the number of virus isolations. In addition, cell culture for AIV isolation has been used. Most recently, real-time reverse transcriptase (RRT)-PCR has been used to detect AIV genome in surveillance samples. The purpose of this study was to develop a diagnostic decision tree that would increase AIV isolations from wild bird surveillance samples when using combinations of detection and isolation methods under our laboratory conditions. Attempts to identify AIV for 50 wild bird surveillance samples were accomplished via isolation in ECE using CAS and YS routes of inoculation, and in Madin-Darby canine kidney (MDCK) cells, and by AIV matrix gene detection using RRT-PCR. AIV was isolated from 36% of samples by CAS inoculation and 46% samples by YS inoculation using ECE, isolated from 20% of samples in MDCK cells, and detected in 54% of the samples by RRT-PCR. The AIV was isolated in ECE in 13 samples by both inoculation routes, five additional samples by allantoic, and 10 additional samples by yolk-sac inoculation, increasing the positive isolation of AIV in ECE to 56%. Allantoic inoculation and RRT-PCR detected AIV in 14 samples, with four additional samples by allantoic route alone and 13 additional samples by RRT-PCR. Our data indicate that addition of YS inoculation of ECE will increase isolation of AIV from wild bird surveillance samples. If we exclude the confirmation RT-PCR test, cost analysis for our laboratory indicates that RRT-PCR is an economical choice for screening samples before doing virus isolation in ECE if the AIV frequency is low in the samples. In contrast, isolation in ECE via CAS and YS inoculation routes without prescreening by RRT-PCR was most efficient and cost-effective if the samples had an expected high frequency of AIV.
Asunto(s)
Aves , Cloaca/virología , Virus de la Influenza A/aislamiento & purificación , Gripe Aviar/virología , Reacción en Cadena de la Polimerasa/veterinaria , Cultivo de Virus/veterinaria , Animales , Animales Salvajes , Línea Celular , Embrión de Pollo , Perros , Heces/virología , Gripe Aviar/epidemiología , Reacción en Cadena de la Polimerasa/economía , Reacción en Cadena de la Polimerasa/métodos , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Cultivo de Virus/economía , Cultivo de Virus/métodosRESUMEN
In the absence of an immunocompetent mouse model for HCV replication, we developed a convenient xenograft mouse model that produces infectious viral particles. For this purpose, HCV-permissive tumors were generated in SCID/beige mice using a tumorigenic population of the human hepatocarcinoma-derived Huh7 cell line. Following infection, HCV RNA increased in the mouse sera and the human tumor by up to 10(5)GE/ml and 10(7)GE/microg of RNA, respectively. Immunohistochemistry analysis revealed that active viral replication had taken place within the tumor. Moreover, virus recovered from infected mice sera was readily infectious in cell culture. Finally, we showed that interferon-alpha and the protease inhibitor BILN-2061 inhibited the cell culture HCVcc strain JFH1 replication in vivo. In conclusion, we developed a simple and inexpensive mouse model that allows the production of infectious HCV particles in vivo. Such a model will be an extremely valuable tool for the characterization of promising drug candidates.
Asunto(s)
Carcinoma Hepatocelular/virología , Hepacivirus/fisiología , Hepatitis C/virología , Neoplasias Hepáticas Experimentales/virología , Modelos Animales , Cultivo de Virus/métodos , Replicación Viral , Animales , Antivirales/farmacología , Línea Celular Tumoral , Femenino , Hepacivirus/efectos de los fármacos , Hepacivirus/genética , Humanos , Interferón-alfa/farmacología , Ratones , Ratones Desnudos , Ratones SCID , Trasplante Heterólogo , Cultivo de Virus/economía , Replicación Viral/efectos de los fármacosRESUMEN
We performed a cost analysis study using decision tree modeling to determine whether the use of multiplex PCR testing for respiratory viruses (xTAG RVP test) is a more or less costly strategy than the status quo testing methods used for the diagnosis of respiratory virus infections in pediatric patients. The decision tree model was constructed by using four testing strategies for respiratory virus detection, viz., direct fluorescent-antibody staining (DFA) alone, DFA plus shell vial culture (SVC), the xTAG RVP test alone, or DFA plus the xTAG RVP test. A review of the charts of 661 pediatric patients was used to determine the length of hospital stay, the number of days in isolation, antibiotic usage, and all other medical procedures performed. The cost of hospitalization by diagnostic status was determined on the basis of the average cost per patient and the number of patients in each arm of the decision tree. The cost per case was the highest for DFA plus SVC at $3,914 (in Canadian dollars), and the lowest was for the xTAG RVP test alone at $3,623, while the costs of DFA alone ($3,911) and DFA plus RVP ($3,849) were intermediate. When all four diagnostic strategies were compared, the least costly strategy was the xTAG RVP test alone when the prevalence of infection was 11% or higher and DFA alone when the prevalence was under 11%. These data indicate a savings of $291 per case investigated if the strategy of using the xTAG RVP test alone was used to replace the status quo test of DFA plus SVC, resulting in a savings of $529,620 per year in direct costs for the four Hamilton, Ontario, Canada, hospitals on the basis of the testing of specimens from 1,820 pediatric inpatients. We conclude that the use of the xTAG RVP test is the least costly strategy for the diagnosis of respiratory virus infections in children and would generate a significant savings for hospitals.
Asunto(s)
Reacción en Cadena de la Polimerasa/economía , Infecciones del Sistema Respiratorio/virología , Virosis/diagnóstico , Virus/aislamiento & purificación , Costos y Análisis de Costo , Humanos , Microscopía Fluorescente/economía , Microscopía Fluorescente/métodos , Ontario , Reacción en Cadena de la Polimerasa/métodos , Cultivo de Virus/economía , Cultivo de Virus/métodos , Virus/genéticaRESUMEN
BACKGROUND: Many laboratories using R-Mix cell lines inoculate other shell vial cultures to improve the recovery of viruses, and in particular, perform terminal hemadsorption (THad) following 10-14 days of incubation to improve detection of respiratory viruses. We explored the cost-effectiveness and added benefits of THad on conventional shell vial cultures from respiratory samples for laboratories using R-Mix cell lines. OBJECTIVES: To determine if eliminating the practice of THad from conventional shell vial culture when R-Mix cultures are negative, would result in a significant reduction in the number of hemadsorbing respiratory viruses detected. STUDY DESIGN: THad results were retrospectively reviewed for 41,129 respiratory shell vial cultures that were set up concurrently with R-Mix cultures. RESULTS: Greater than 95% of hemadsorbing respiratory viruses were recovered by R-Mix standard protocol within 24h of inoculation, and only 5% were detected by THad at 10-14 days. CONCLUSION: The practice of hemadsorption at days 10-14 for conventional shell vial cultures from respiratory specimens should be discontinued for laboratories using R-Mix due to its low yield, questionable clinical impact of delayed results and additional costs.
Asunto(s)
Hemabsorción , Infecciones del Sistema Respiratorio/virología , Virosis/diagnóstico , Virus/aislamiento & purificación , Línea Celular , Humanos , Cultivo de Virus/economía , Cultivo de Virus/métodosRESUMEN
Nucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species, suggesting that, when nucleic acid amplification testing is performed, viral culture may have no additional benefit.
Asunto(s)
Enterovirus/aislamiento & purificación , Herpesviridae/aislamiento & purificación , Técnicas de Amplificación de Ácido Nucleico , Análisis Costo-Beneficio , Enterovirus/genética , Infecciones por Enterovirus/líquido cefalorraquídeo , Herpesviridae/genética , Infecciones por Herpesviridae/líquido cefalorraquídeo , Humanos , Técnicas de Amplificación de Ácido Nucleico/economía , Cultivo de Virus/economía , Cultivo de Virus/métodosRESUMEN
Cervical cancer screening with human papillomavirus (HPV) DNA testing has potential advantages over conventional, smear testing in that it can predict cases in which invasive cancers are more likely to develop, may be cheaper to implement and improve compliance. In areas of the world where little formalized cervical cancer screening takes place, or where health resources are limited, HPV testing has been suggested as a possible alternative for primary screening. In this paper we demonstrate the use of mathematical modelling to evaluate the effects of setting up screening programmes in Eastern Europe with HPV DNA testing as the primary screening tool and compare it with conventional smear testing. The impact of screening is measured in terms of the life years gained and the resulting resource usage and cost. We investigate several screening options with different screening intervals and age ranges for the target population.
Asunto(s)
Planificación en Salud/organización & administración , Tamizaje Masivo/métodos , Papillomaviridae , Infecciones por Papillomavirus/complicaciones , Infecciones por Papillomavirus/diagnóstico , Infecciones Tumorales por Virus/complicaciones , Infecciones Tumorales por Virus/diagnóstico , Enfermedades del Cuello del Útero/complicaciones , Enfermedades del Cuello del Útero/diagnóstico , Neoplasias del Cuello Uterino/diagnóstico , Neoplasias del Cuello Uterino/virología , Frotis Vaginal , Cultivo de Virus , Adulto , Colposcopía/economía , Colposcopía/psicología , Progresión de la Enfermedad , Europa Oriental/epidemiología , Femenino , Humanos , Comercialización de los Servicios de Salud , Tamizaje Masivo/economía , Tamizaje Masivo/psicología , Persona de Mediana Edad , Estadificación de Neoplasias , Cooperación del Paciente , Valor Predictivo de las Pruebas , Neoplasias del Cuello Uterino/mortalidad , Frotis Vaginal/economía , Frotis Vaginal/psicología , Valor de la Vida , Cultivo de Virus/economíaRESUMEN
OBJECTIVE: To compare MRC-5 and primary rabbit kidney (PRK) cells to either cell monolayer alone for the recovery of herpes simplex virus (HSV). DESIGN: A total of 2476 specimens received for HSV culture during a 3-year period were cultured on MRC-5 and PRK cells. Detection rates and the time to first detection were determined for each cell type used. A cost analysis was also performed for the isolation and identification of HSV using both cell types, the MRC-5 cell line alone, and PRK cell culture alone. SETTING: Large, urban, tertiary-care, university-affiliated hospital. RESULTS: Of the 2476 specimens cultured for HSV, 535 (21.6%) were positive. The MRC-5 cell line detected 531 (99.3%), and the PRK cell culture detected 522 (97.6%) of the positive specimens. Thirteen HSV isolates were detected only in MRC-5 cells, and four were isolated only in PRK cells. Approximately 44% of the cultures were positive by day 1, 84% by day 2, and 98% by day 3, regardless of the cell type used. The total cost per culture was comparable for MRC-5 and PRK cells. CONCLUSIONS: There was no difference in the sensitivity or time to detection of HSV between PRK and MRC-5 cells either alone or in combination. Either cell type alone represents an efficient, cost-effective method for the isolation of HSV.
Asunto(s)
Herpes Simple/diagnóstico , Simplexvirus/aislamiento & purificación , Cultivo de Virus/métodos , Animales , Línea Celular , Células Cultivadas , Costos y Análisis de Costo , Humanos , Riñón/citología , Conejos , Sensibilidad y Especificidad , Simplexvirus/crecimiento & desarrollo , Ensayo de Placa Viral , Cultivo de Virus/economíaRESUMEN
Qualitative human immunodeficiency virus culture is a slow, labor-intensive, and expensive procedure, yet critical for the diagnosis of infants born to human immunodeficiency virus-seropositive mothers. We report that the cultures can be terminated at day 21 with minimal false-negative results but with considerable savings in both time and money.
Asunto(s)
Infecciones por VIH/diagnóstico , Cultivo de Virus/normas , Niño , Preescolar , Ensayos Clínicos como Asunto , Conferencias de Consenso como Asunto , Reacciones Falso Negativas , Femenino , Infecciones por VIH/transmisión , Humanos , Lactante , Recién Nacido , Transmisión Vertical de Enfermedad Infecciosa , Factores de Tiempo , Cultivo de Virus/economíaRESUMEN
A simultaneous triple culture for adenovirus, cytomegalovirus, and herpes simplex virus, using three different fluorochromes for virus detection and differentiation in the same shell vial, was developed. In evaluations the triple culture performed comparably to separate cultures, required less specimen volume, and was less expensive to perform.