RESUMEN
Recent developments of novel electron diffraction techniques have shown to be powerful for determination of atomic resolution structures from micron- and nano-sized crystals, too small to be studied by single-crystal X-ray diffraction. In this work, the structure of a rare lysozyme polymorph is solved and refined using continuous rotation MicroED data and standard X-ray crystallographic software. Data collection was performed on a standard 200 kV transmission electron microscope (TEM) using a highly sensitive detector with a short readout time. The data collection is fast (â¼3 min per crystal), allowing multiple datasets to be rapidly collected from a large number of crystals. We show that merging data from 33 crystals significantly improves not only the data completeness, overall I/σ and the data redundancy, but also the quality of the final atomic model. This is extremely useful for electron beam-sensitive crystals of low symmetry or with a preferred orientation on the TEM grid.
Asunto(s)
Cristalografía/estadística & datos numéricos , Procesamiento de Imagen Asistido por Computador/estadística & datos numéricos , Microscopía Electrónica de Transmisión/estadística & datos numéricos , Muramidasa/química , Animales , Pollos , Cristalización , Cristalografía/métodos , Conjuntos de Datos como Asunto , Clara de Huevo/química , Microscopía Electrónica de Transmisión/instrumentación , Microscopía Electrónica de Transmisión/métodos , Modelos Moleculares , Conformación Proteica , Pliegue de Proteína , Programas InformáticosAsunto(s)
Química/instrumentación , Química/organización & administración , Cristalografía/instrumentación , Cristalografía/estadística & datos numéricos , Formulación de Políticas , Química/economía , Química/tendencias , Cristalografía/economía , Cristalografía/tendencias , Cooperación Internacional , Física/organización & administración , Física/tendencias , Política , Apoyo a la Investigación como Asunto , Sincrotrones/economía , Sincrotrones/estadística & datos numéricosRESUMEN
The number of macromolecular structures deposited in the Protein Data Bank now approaches 100,000, with the vast majority of them determined by crystallographic methods. Thousands of papers describing such structures have been published in the scientific literature, and 20 Nobel Prizes in chemistry or medicine have been awarded for discoveries based on macromolecular crystallography. New hardware and software tools have made crystallography appear to be an almost routine (but still far from being analytical) technique and many structures are now being determined by scientists with very limited experience in the practical aspects of the field. However, this apparent ease is sometimes illusory and proper procedures need to be followed to maintain high standards of structure quality. In addition, many noncrystallographers may have problems with the critical evaluation and interpretation of structural results published in the scientific literature. The present review provides an outline of the technical aspects of crystallography for less experienced practitioners, as well as information that might be useful for users of macromolecular structures, aiming to show them how to interpret (but not overinterpret) the information present in the coordinate files and in their description. A discussion of the extent of information that can be gleaned from the atomic coordinates of structures solved at different resolution is provided, as well as problems and pitfalls encountered in structure determination and interpretation.
Asunto(s)
Cristalografía/métodos , Proteínas/química , Cristalografía/estadística & datos numéricos , Cristalografía por Rayos X/métodos , Cristalografía por Rayos X/estadística & datos numéricos , Bases de Datos de Proteínas , Modelos Moleculares , Estructura Molecular , Extractos Vegetales , Conformación Proteica , Proteínas/genética , Proteínas/aislamiento & purificación , Programas Informáticos , Electricidad EstáticaRESUMEN
The FFT calculation of spherical harmonics, Wigner D matrices and rotation function has been extended to all angular variables in the AMoRe molecular replacement software. The resulting code avoids singularity issues arising from recursive formulas, performs faster and produces results with at least the same accuracy as the original code. The new code aims at permitting accurate and more rapid computations at high angular resolution of the rotation function of large particles. Test calculations on the icosahedral IBDV VP2 subviral particle showed that the new code performs on the average 1.5 times faster than the original code.
Asunto(s)
Cristalografía/estadística & datos numéricos , Algoritmos , Fenómenos Químicos , Química Física , Análisis de Fourier , Virus de la Enfermedad Infecciosa de la Bolsa/química , Rotación , Programas Informáticos , Proteínas Estructurales Virales/químicaRESUMEN
An automated high-throughput dispenser has been developed for the setup of protein crystallization trials by vapor diffusion or Microbatch methods. The Hydra-Plus-One is composed of a Hydra-PP system equipped with a motorized XYZ-platform, 96 precision glass syringes and a single-channel microsolenoid dispenser, which transfers 100 nl-50 micro l of protein solution with an accuracy of > 90% at a speed of 60s per 96 wells. Up to 300 micro l of premixed cocktails can be aspirated with the 96-syringe-assembly and dispensed into reservoir and droplet wells within 60s. The Hydra-Plus-One combines high precision, reliability and speed in a cost-effective high-throughput system ideally suited for protein crystallization