RESUMEN
This cross-sectional study aimed to identify and quantify up to 42 target species colonizing the early biofilm of dental implants restored with titanium or zirconia abutments. A total of 720 samples from 20 healthy individuals were investigated. Biofilm samples were collected from the peri-implant sulci, inner parts of implants, abutment surfaces and prosthetic crowns over a functioning period of 30 days. Checkerboard DNA-DNA hybridization was used for microbial detection and quantitation. Clinical characteristics (probing depth, bleeding on probing, clinical attachment level and marginal bone loss) were also investigated during the monitoring period. Genome counts were low at the implant loading time point for both the abutment materials, and increased over time. Both the titanium and the zirconia groups presented similar microbial counts and diversity over time, and the microbiota was very similar to that colonizing the remaining teeth. Clinical findings were consistent with a healthy condition with no significant difference regarding marginal bone loss between the two materials.
Asunto(s)
Biopelículas/crecimiento & desarrollo , Pilares Dentales/microbiología , Implantes Dentales/microbiología , Genoma Bacteriano , Microbiota/genética , Titanio/química , Circonio/química , Adhesión Bacteriana , Coronas/microbiología , HumanosRESUMEN
Prosthetic restorations that have been tried in the patient's mouth are potential sources of infection. In order to avoid cross-infection, protocols for infection control should be established in dental office and laboratory. This study evaluated the antimicrobial efficacy of disinfectants on full metal crowns contaminated with microorganisms. Full crowns cast in a Ni-Cr alloy were assigned to one control group (n=6) and 5 experimental groups (n=18). The crowns were placed in flat-bottom glass balloons and were autoclaved. A microbial suspension of each type of strain - S. aureus, P. aeruginosa, S. mutans, E. faecalis and C. albicans- was aseptically added to each experimental group, the crowns being allowed for contamination during 30 min. The contaminated specimens were placed into recipients with the chemical disinfectants (1% and 2% sodium hypochlorite and 2% glutaraldehyde) for 5, 10 and 15 min. Thereafter, the crowns were placed into tubes containing different broths and incubated at 35ºC. The control specimens were contaminated, immersed in distilled water for 20 min and cultured in Thioglycollate broth at 35ºC. Microbial growth assay was performed by qualitative visual examination after 48 h, 7 and 12 days. Microbial growth was noticed only in the control group. In the experimental groups, turbidity of the broths was not observed, regardless of the strains and immersion intervals, thus indicating absence of microbial growth. In conclusion, all chemical disinfectants were effective in preventing microbial growth onto full metal crowns.
Asunto(s)
Antiinfecciosos/farmacología , Coronas/microbiología , Descontaminación/métodos , Desinfectantes Dentales/química , Infección Hospitalaria/prevención & control , Aleaciones Dentales , Contaminación de Equipos , Glutaral/farmacología , Humanos , Hipoclorito de Sodio/farmacologíaRESUMEN
Prosthetic restorations that have been tried in the patient's mouth are potential sources of infection. In order to avoid cross-infection, protocols for infection control should be established in dental office and laboratory. This study evaluated the antimicrobial efficacy of disinfectants on full metal crowns contaminated with microorganisms. Full crowns cast in a Ni-Cr alloy were assigned to one control group (n=6) and 5 experimental groups (n=18). The crowns were placed in flat-bottom glass balloons and were autoclaved. A microbial suspension of each type of strain - S. aureus, P. aeruginosa, S. mutans, E. faecalis and C. albicans- was aseptically added to each experimental group, the crowns being allowed for contamination during 30 min. The contaminated specimens were placed into recipients with the chemical disinfectants (1 percent and 2 percent sodium hypochlorite and 2 percent glutaraldehyde) for 5, 10 and 15 min. Thereafter, the crowns were placed into tubes containing different broths and incubated at 35ºC. The control specimens were contaminated, immersed in distilled water for 20 min and cultured in Thioglycollate broth at 35ºC. Microbial growth assay was performed by qualitative visual examination after 48 h, 7 and 12 days. Microbial growth was noticed only in the control group. In the experimental groups, turbidity of the broths was not observed, regardless of the strains and immersion intervals, thus indicating absence of microbial growth. In conclusion, all chemical disinfectants were effective in preventing microbial growth onto full metal crowns.
Restaurações protéticas provadas na cavidade bucal dos pacientes são fontes potenciais de infecção. Para evitar infecção cruzada, protocolos de controle de infecção devem ser estabelecidos no consultório e laboratório odontológicos. Este estudo avaliou a eficácia antimicrobiana de desinfetantes químicos em coroas metálicas contaminadas com microorganismos. Coroas totais fundidas com liga de Ni-Cr foram divididas em grupo controle (n=6) e 5 grupos experimentais (n=18). As coroas foram colocadas em balões de vidro e esterilizadas em autoclave. A suspensão microbiana de cada tipo de cepa (S. aureus, P. aeruginosa, S. mutans, E. faecalis e C. albicans) foi assepticamente adicionada a cada grupo experimental, e as coroas foram deixadas contaminar por 30 min. Os corpos-de-prova contaminados foram colocados em recipientes com os desinfetantes químicos (hipoclorito de sódio 1 por cento e 2 por cento e glutaraldeído) por 5, 10 e 15 min. A seguir, as coroas foram colocadas em tubos contendo diferentes meios de cultura e incubadas a 35ºC. Os corpos-de-prova do grupo controle foram contaminados, imersos em água destilada por 20 min e a seguir colocados em tubos de ensaio com meio de cultura Thioglycollate e incubados a 35ºC. A análise do crescimento microbiano foi realizada pelo exame visual qualitativo após 48 h, 7 e 12 dias. Houve crescimento microbiano apenas no grupo controle. No grupo experimental não foi observada turvação dos meios de cultura, independentemente das cepas e períodos de imersão. Conclui-se que todos desinfetantes químicos foram eficazes para prevenir o crescimento microbiano.
Asunto(s)
Humanos , Antiinfecciosos/farmacología , Coronas/microbiología , Descontaminación/métodos , Desinfectantes Dentales/química , Infección Hospitalaria/prevención & control , Aleaciones Dentales , Contaminación de Equipos , Glutaral/farmacología , Hipoclorito de Sodio/farmacologíaRESUMEN
The aim of this study was to evaluate retrospectively the periodontal conditions of teeth with fixed crowns that had been in place from 3 to 5 years before the study was conducted. Forty individuals were recalled for a follow-up visit. Full-mouth clinical examinations were carried out and Visible Plaque Index (VPI), Gingival Bleeding Index (GBI), Probing Pocket Depth (PPD), and clinical attachment level (CAL) were assessed in 6 sites per tooth. Parallel radiographs were also taken and blindly analyzed by a digital caliper (distance between the apex and the bone crest). BANA tests were performed. A contra-lateral sound tooth was considered the control. Mean values were obtained and Wilcoxon and paired sample t tests were used to compare the test and control sites. Crowns had a mean VPI value of 30.42% as compared to 49.17% for sound teeth. The GBI was 33.33% and 26.25% for test and control teeth respectively. Assessment of PPD revealed values of 2.30 and 2.14 mm, and assessment of CAL revealed averages of 2.02 and 1.89 mm for test and control teeth respectively. The mean values for radiographic distances were 12.73 and 13.67 mm, and for the BANA test, 67.50 and 50.00 for sound and crowned teeth, respectively. Statistically significant differences were observed for all parameters except for CAL and for the BANA test. It may be concluded that, with the methods used in the present study, crowns may be associated with more signs of inflammation, however not with periodontal breakdown.
Asunto(s)
Coronas , Índice de Placa Dental , Placa Dental/diagnóstico , Enfermedades Periodontales , Adulto , Benzoilarginina-2-Naftilamida , Coronas/efectos adversos , Coronas/microbiología , Placa Dental/diagnóstico por imagen , Placa Dental/microbiología , Femenino , Hemorragia Gingival/microbiología , Humanos , Masculino , Persona de Mediana Edad , Enfermedades Periodontales/diagnóstico , Enfermedades Periodontales/diagnóstico por imagen , Enfermedades Periodontales/microbiología , Índice Periodontal , Bolsa Periodontal/microbiología , Radiografía , Reproducibilidad de los Resultados , Estudios Retrospectivos , Estadísticas no ParamétricasRESUMEN
The aim of this study was to evaluate retrospectively the periodontal conditions of teeth with fixed crowns that had been in place from 3 to 5 years before the study was conducted. Forty individuals were recalled for a follow-up visit. Full-mouth clinical examinations were carried out and Visible Plaque Index (VPI), Gingival Bleeding Index (GBI), Probing Pocket Depth (PPD), and clinical attachment level (CAL) were assessed in 6 sites per tooth. Parallel radiographs were also taken and blindly analyzed by a digital caliper (distance between the apex and the bone crest). BANA tests were performed. A contra-lateral sound tooth was considered the control. Mean values were obtained and Wilcoxon and paired sample t tests were used to compare the test and control sites. Crowns had a mean VPI value of 30.42 percent as compared to 49.17 percent for sound teeth. The GBI was 33.33 percent and 26.25 percent for test and control teeth respectively. Assessment of PPD revealed values of 2.30 and 2.14 mm, and assessment of CAL revealed averages of 2.02 and 1.89 mm for test and control teeth respectively. The mean values for radiographic distances were 12.73 and 13.67 mm, and for the BANA test, 67.50 and 50.00 for sound and crowned teeth, respectively. Statistically significant differences were observed for all parameters except for CAL and for the BANA test. It may be concluded that, with the methods used in the present study, crowns may be associated with more signs of inflammation, however not with periodontal breakdown.
O objetivo deste estudo foi avaliar retrospectivamente as condições periodontais de pacientes com coroas fixas colocadas de 3 a 5 anos antes da realização da pesquisa. Quarenta indivíduos foram rechamados. Exames clínicos de toda a boca foram realizados avaliando-se Índice de Placa Visível (IPV), Índice de Sangramento Gengival (ISG), Profundidade de Sondagem (PS) e Nível Clínico de Inserção (NCI) em 6 sítios por dente. Radiografias paralelas foram obtidas e analisadas cegamente por paquímetro digital (distância do ápice à crista óssea). Testes BANA foram realizados. Um dente hígido contralateral foi considerado como controle. Valores médios foram obtidos e testes de Wilcoxon e t pareado foram utilizados para comparar os sítios teste e controle. Coroas apresentaram um valor médio de IPV de 30,42 por cento, comparado com 49,17 por cento para dentes hígidos. O ISG foi de 33,33 por cento e 26,25 por cento para dentes com coroas e hígidos, respectivamente. A PS revelou valores de 2,30 e 2,14 mm e a análise do NCI demonstrou médias de 2,02 e 1,89 mm para dentes teste e controle, respectivamente. Os valores médios para as distâncias radiográficas foram de 12,73 e 13,67 mm, e para o teste BANA, de 67,50 e 50,00 para dentes hígidos e com coroas, respectivamente. Diferenças estatisticamente significantes foram observadas para todos os parâmetros, exceto para NCI e para o teste BANA. Pode-se concluir que, com os métodos utilizados no presente estudo, coroas podem ser associadas a mais sinais de inflamação, entretanto não com destruição periodontal.
Asunto(s)
Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad , Coronas , Índice de Placa Dental , Placa Dental/diagnóstico , Enfermedades Periodontales , Coronas/efectos adversos , Coronas/microbiología , Placa Dental/microbiología , Placa Dental , Hemorragia Gingival/microbiología , Índice Periodontal , Enfermedades Periodontales/diagnóstico , Enfermedades Periodontales/microbiología , Enfermedades Periodontales , Bolsa Periodontal/microbiología , Reproducibilidad de los Resultados , Estudios Retrospectivos , Estadísticas no ParamétricasRESUMEN
Las grades falencias presentadas en la confección de coronas y puentes, como consecuencia de la resina acrílica común, hoy son superadas totalmente con el uso de la resina mejorada BIOLON y su aplicación a través de la técnica del pincelado, de fácil y práctica manipulación, permitiendo efectuar caracterizaciones y modificaciones, con el margen de tiempo suficiente para que a criterio y según el caso aconseje, el operador las realice y obtenga una corona casi original, Se ha comprobado, la indispensable necesidad del uso de materiales de precisión en la confección de ytrabajos protéticos, y aconsejamos el uso del BIOLON THE L.D.CAULK COMPANY.Fracc.dentsply Argentina y Americana