RESUMEN
In food industry, Listeria monocytogenes contamination can occur accidentally despite the quality control of raw materials and factory. Decontamination processes or inhibitory effects of ingredients/additives in food products are set up to ensure compliance with hygiene and microbiological criteria. These actions represent stresses for the pathogenic agent, causing fluctuations in its physiological states. Moreover, during these environmental stresses, Listeria monocytogenes can enter in a viable but nonculturable (VBNC) state which is not detected by plate counting but by flow cytometry. This technique coupled with cell staining by fluorescent dyes offers the possibility to assess different physiological states based on different cellular parameters: enzymatic activity, transmembrane integrity, membrane potential, and respiratory activity. In this chapter, we present a method to assess the viability of foodborne pathogens using a double-staining principle based on the assessment of membrane integrity and intracellular esterase activity.
Asunto(s)
Citometría de Flujo , Listeria monocytogenes , Viabilidad Microbiana , Listeria monocytogenes/crecimiento & desarrollo , Listeria monocytogenes/fisiología , Citometría de Flujo/métodos , Microbiología de Alimentos/métodos , Colorantes Fluorescentes/química , Coloración y Etiquetado/métodos , Membrana Celular/metabolismoRESUMEN
Vanillin is a commonly used synthetic flavoring agent in daily life. However, excessive intake of vanillin may pose risks to human health. Therefore, there is an urgent need for rapid and sensitive detection methods for vanillin. In this study, we developed a fluorescent sensor based on Cd-MOF for the sensitive and selective recognition of vanillin. The presence of vanillin leads to significant fluorescence quenching of Cd-MOF due to competitive absorption and photoinduced electron transfer (PET). The limit of detection was determined to be 39.6 nM, which is the lowest-among the reported fluorescent probes. The sensor was successfully applied for the detection of vanillin in real samples such as powdered milk and milk, with a recovery rate ranging from 96.88 % to 104.83 %. Furthermore, by immobilizing the Cd-MOF probe into a polyvinyl alcohol (PVA) film, we achieved a portable and visual detection composite materials for vanillin.
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Benzaldehídos , Estructuras Metalorgánicas , Leche , Espectrometría de Fluorescencia , Benzaldehídos/análisis , Benzaldehídos/química , Leche/química , Animales , Espectrometría de Fluorescencia/métodos , Estructuras Metalorgánicas/química , Polvos , Colorantes Fluorescentes/química , Límite de Detección , Cadmio/análisisRESUMEN
Alcoholic liver disease (ALD) is a chronic toxic liver injury caused by long-term heavy drinking. Due to the increasing incidence, ALD is becoming one of important medical tasks. Many studies have shown that the main mechanism of liver damage caused by large amounts of alcohol may be related to antioxidant stress. As an important antioxidant, cysteine (Cys) is involved in maintaining the normal redox balance and detoxifying metabolic function of the liver, which may be closely related to the pathogenesis of ALD. Therefore, it is necessary to develop a simple non-invasive method for rapid monitoring of Cys in liver. Thus, a near-infrared (NIR) fluorescent probe DCI-Ac-Cys which undergoes Cys triggered cascade reaction to form coumarin fluorophore is developed. Using the DCI-Ac-Cys, decreased Cys was observed in the liver of ALD mice. Importantly, different levels of Cys were monitored in the livers of ALD mice taking silybin and curcumin with the antioxidant effects, indicating the excellent therapeutic effect on ALD. This study provides the important references for the accurate diagnosis of ALD and the pharmacodynamic evaluation of silybin and curcumin in the treatment of ALD, and support new ideas for the pathogenesis of ALD.
Asunto(s)
Cumarinas , Cisteína , Colorantes Fluorescentes , Hepatopatías Alcohólicas , Animales , Cisteína/análisis , Cisteína/metabolismo , Cumarinas/química , Colorantes Fluorescentes/química , Hepatopatías Alcohólicas/metabolismo , Hepatopatías Alcohólicas/patología , Masculino , Hígado/metabolismo , Hígado/efectos de los fármacos , Hígado/patología , Ratones , Ratones Endogámicos C57BL , Espectroscopía Infrarroja Corta/métodos , Curcumina/farmacología , Espectrometría de Fluorescencia , Silibina/farmacología , Silibina/químicaRESUMEN
Epilepsy is one of the most commonly-seen neurological disorders, and both endoplasmic reticulum stress (ERS) and oxidative stress (OS) have been demonstrated to be associated with epileptic seizures. As one of the three endogenous thiol-containing amino acids, cysteine (Cys) is recognized not only as an important biomarker of various biological processes but also widely used as a significant additive in the food industry. However, the exact role that Cys plays in ERS has not been well answered up to now. In this paper, we reported the first flavone-based fluorescent probe (namely BFC) with nice endoplasmic reticulum (ER)-targeting ability, which was capable of monitoring Cys in a fast response (3.0 min), large stokes shift (130 nm) and low detection limit (10.4 nM). The recognition mechanism of Cys could be attributed to the addition-cyclization reaction involving a Cys residue and an acrylate group, resulting in the release of the strong excited-state intramolecular proton transfer (ESIPT) emission molecule of benzoflavonol (BF). The low cytotoxicity and good biocompatibility of the probe BFC allowed for monitoring the fluctuation of endogenous Cys levels under both ERS and OS processes, as well as in zebrafish models of epilepsy. Quantitative determination of Cys with the probe BFC was also achieved in three different food samples. Additionally, a probe-immersed test strips integrated with a smartphone device was successfully constructed for on-site colorimetric detection of Cys. Undoubtedly, our work provided a valuable tool for tracking Cys levels in both an epilepsy model and real food samples.
Asunto(s)
Cisteína , Retículo Endoplásmico , Epilepsia , Flavonas , Colorantes Fluorescentes , Análisis de los Alimentos , Pez Cebra , Colorantes Fluorescentes/química , Cisteína/análisis , Animales , Epilepsia/diagnóstico , Flavonas/análisis , Flavonas/química , Retículo Endoplásmico/metabolismo , Análisis de los Alimentos/métodos , Espectrometría de Fluorescencia/métodos , Humanos , Modelos Animales de Enfermedad , Límite de Detección , Estrés del Retículo EndoplásmicoRESUMEN
The role of ClO- in the physiological functioning of organisms is significant. In this paper, the four fluorescent probes HONx (HON1, HON2, HON3 and HON4) were prepared based on oxyanthracene through the introduction of different substituents, and their photophysical properties were investigated, among which the AIE effect of HON1 was the most significant, and therefore the fluorescent "turn-off" ClO- probe HON1-CN was chosen to be prepared by constructing the ClO- recognition site hydrazone bond at HON1. The ClO- recognises the hydrazone group in the probe HON1-CN, and when the hydrazone bond is broken, the aldehyde group is released, generating HON1 with yellow fluorescence. The probe HON1-CN is highly selective and stable for the detection of ClO- with a detection limit of 0.48 µM and a more than 10-fold increase in fluorescence intensity when the fluorescence is 'switched on', and to a lesser extent, the probe is also very good for the detection of hypochlorite ClO- in the pericarp. Finally, HON1-CN has also been used to detect the presence of ClO- in HeLa cells and zebrafish.
Asunto(s)
Colorantes Fluorescentes , Ácido Hipocloroso , Espectrometría de Fluorescencia , Xantonas , Pez Cebra , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Xantonas/química , Animales , Ácido Hipocloroso/análisis , Humanos , Células HeLa , Frutas/química , Límite de DetecciónRESUMEN
Mercury ion (Hg2+), a heavy metal cation with greater toxicity, is widely present in the ecological environment and has become a serious threat to human health and environmental safety. Currently, developing a solution to simultaneously visualize and monitor Hg2+ in environmental samples, including water, soil, and plants, remains a great challenge. In this work, we created and synthesized a near-infrared fluorescent probe, BBN-Hg, and utilized Hg2+ to trigger the partial cleavage of the carbon sulfate ester in BBN-Hg as a sensing mechanism, and the fluorescence intensity of BBN-Hg was significantly enhanced at 650 nm, thus realizing the visualization of Hg2+ with good selectivity (detection limit, 53 nM). In live cells and zebrafish, the probe BBN-Hg enhances the red fluorescence signal in the presence of Hg2+, and successfully performs 3D imaging on zebrafish, making it a powerful tool for detecting Hg2+ in living systems. More importantly, with BBN-Hg, we are able to detect Hg2+ in actual water samples, soil and plant seedling roots. Furthermore, the probe was prepared as a test strip for on-site determination of Hg2+ with the assistance of a smartphone. Therefore, this study offers an easy-to-use and useful method for tracking Hg2+ levels in living organisms and their surroundings.
Asunto(s)
Colorantes Fluorescentes , Mercurio , Pez Cebra , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Mercurio/análisis , Animales , Humanos , Espectrometría de Fluorescencia/métodos , Límite de DetecciónRESUMEN
Fluoride ions (F-) are one of the essential trace elements for the human body and are widely existed in nature. In this study, we present a novel fluorescent probe (YF-SZ-F) designed and synthesized for the specific detection of F-. The probe exhibits high sensitivity, excellent selectivity, and low cytotoxicity, making it a promising tool for biomedical applications. Imaging experiments conducted on zebrafish and Arabidopsis roots demonstrate the probe's remarkable cellular permeability and biocompatibility, laying a solid foundation for its potential biomedical utility. Furthermore, the probe holds potential for practical applications in environmental monitoring and public health through its capability to detect fluoride ions in water samples and via mobile phone software. This multifaceted functionality underscores the broad applicability and significance of the fluorescent probe, not only in scientific research but also in real-world scenarios, contributing to the development of more convenient and precise methods for fluoride detection.
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Benzotiazoles , Colorantes Fluorescentes , Fluoruros , Pez Cebra , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Fluoruros/análisis , Animales , Benzotiazoles/química , Humanos , Arabidopsis/química , Espectrometría de Fluorescencia/métodos , Imagen ÓpticaRESUMEN
N-acetyl-L-cysteine (NAC) as a class of thiols is commonly used in the treatment of lung diseases, detoxification and prevention of liver damage. In this paper, 4-mercaptobenzoic acid (4-MBA) coated and polyvinylpyrrolidone (PVP) attached copper nanoclusters (4-MBA@PVP-CuNCs) were successfully synthesized using a simple one-pot method with an absolute quantum yield of 10.98 %, and its synthetic conditions (like effects of single/double ligands and temperature) were studied intensively. Then Hg2+ could quench the fluorescence of the 4-MBA@PVP-CuNCs and its fluorescence was restored with the addition of NAC. Based on the above principles, an off-on switching system was established to detect NAC. That is, the 4-MBA@PVP-CuNCs-Hg probe was prepared by adding Hg2+ to switch off the fluorescence of the CuNCs by static quenching, and then NAC was added to switch on the fluorescence of the probe based on the chelation of NAC and Hg2+. Moreover, the effects of metal ion types and mercury ion doses for the probe construction were also further discussed. The method showed excellent linearity in the range of 0.05-1.25 µM and low detection limit of 16 nM. Meanwhile, good recoveries in real urine, tablets and pellets were observed, which proved the reliability of the method and provided a convenient, fast and sensitive method for NAC detection.
Asunto(s)
Acetilcisteína , Cobre , Límite de Detección , Nanopartículas del Metal , Espectrometría de Fluorescencia , Compuestos de Sulfhidrilo , Acetilcisteína/química , Acetilcisteína/orina , Cobre/química , Cobre/análisis , Espectrometría de Fluorescencia/métodos , Compuestos de Sulfhidrilo/química , Compuestos de Sulfhidrilo/análisis , Ligandos , Nanopartículas del Metal/química , Mercurio/análisis , Mercurio/orina , Humanos , Colorantes Fluorescentes/química , Povidona/química , Benzoatos/química , Polímeros/químicaRESUMEN
As a reactive sulfur species, sulfur dioxide (SO2) and its derivatives play crucial role in various physiological processes, which can maintain redox homeostasis at normal levels and lead to the occurrence of many diseases at abnormal levels. So, the development of a suitable fluorescent probe is a crucial step in advancing our understanding of the role of SO2 derivatives in living organisms. Herein, we developed a near-infrared fluorescent probe (SP) based on the ICT mechanism to monitor SO2 derivatives in living organisms in a ratiometric manner. The probe SP exhibited excellent selectivity, good sensitivity, fast response rate (within 50 s), and low detection limit (1.79 µM). In addition, the cell experiment results suggested that the SP has been successfully employed for the real-time monitoring of endogenous and exogenous SO2 derivatives with negligible cytotoxicity. Moreover, SP was effective in detecting SO2 derivatives in mice.
Asunto(s)
Colorantes Fluorescentes , Dióxido de Azufre , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Dióxido de Azufre/análisis , Animales , Ratones , Humanos , Límite de Detección , Espectrometría de Fluorescencia , Imagen Óptica , Células HeLaRESUMEN
Tyramine signaling amplification (TSA) technology is generally applied in immunofluorescence, enzyme-linked immunoassays, in situ hybridization techniques, etc. Successful amplification of fluoresence signals cannot be achieved without excellent fluorescent dyes. BODIPY fluorophore is an ideal probe for cell fluorescence imaging, but pristine BODIPY cannot be direct used in the TSA system. In the paper, the new red-shifted tyramide-conjugated BODIPY (BDP-B/C/D) was synthesized via the Knoevenagel condensation reaction, which based on the tyramide-conjugated BODIPY (BDP-A). The synthesized dyes were combined with tyramine to obtain which could be used as a fluorescent substrate for enzymatic reaction of TSA. By using the selected substrate (BDP-C) in TSA, we found it to be more sensitive than the commercial dye 594 styramide for the detection of low-abundance antigen proteins.
Asunto(s)
Compuestos de Boro , Colorantes Fluorescentes , Porfobilinógeno , Tiramina , Tiramina/química , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Humanos , Compuestos de Boro/química , Compuestos de Boro/síntesis química , Porfobilinógeno/análogos & derivados , Porfobilinógeno/química , Células HeLa , Espectrometría de Fluorescencia , Imagen ÓpticaRESUMEN
Sodium tripolyphosphate (STPP), as one of the many food additives, can cause gastrointestinal discomfort and a variety of adverse reactions when ingested by the human body, which is a great potential threat to human health. Therefore, it is necessary to develop a fast, sensitive and simple method to detect STPP in food. In this study, we synthesized a kind of nitrogen-doped carbon quantum dots (N-CQDs), and were surprised to find that the addition of STPP led to the gradual enhancement of the emission peaks of the N-CQDs, with a good linearity in the range of 0.067-1.96 µM and a low detection limit as low as 0.024 µM. Up to now, there is no report on the use of carbon quantum dots for the direct detection of STPP. Meanwhile, we found that the addition of Al3+ effectively bursts the fluorescence intensity of N-CQDs@STPP solution and has a good linear relationship in the range of 0.33-6.25 µM with a lower detection limit of 0.24 µM. To this end, we developed a fluorescent probe to detect STPP and Al3+. In addition, the probe was successfully applied to the detection of bread samples, which has great potential for practical application.
Asunto(s)
Carbono , Colorantes Fluorescentes , Aditivos Alimentarios , Límite de Detección , Polifosfatos , Puntos Cuánticos , Espectrometría de Fluorescencia , Puntos Cuánticos/química , Colorantes Fluorescentes/química , Aditivos Alimentarios/análisis , Espectrometría de Fluorescencia/métodos , Carbono/química , Polifosfatos/análisis , Polifosfatos/química , Aluminio/análisis , Nitrógeno/química , Pan/análisisRESUMEN
A green, simple and sensitive spectrofluorometric approach for determining vonoprazan fumarate in bulk and pharmaceutical dosage form by turning off the fluorescence of sodium salicylate is developed. The addition of vonoprazan fumarate reduced linearly the fluorescence intensity of 0.4 mM sodium salicylate at λem 408 nm and at λex 330 nm. The approach was found to be linear in the 50.0-3000.0 ng/mL range. The limits of detection and quantification were 10.97 and 33.23 ng/mL, respectively. The presented method proved its suitability in determination of vonoprazan fumarate in its pure and pharmaceutical dosage form. This method employs water as the exclusive solvent and utilizes safe reagents, evaluated using the Analytical Eco Scale, Green Analytical Procedure Index (GAPI), and carbon footprint. In contrast, previous methods relied on toxic reagents and required extended heating times, resulting in higher environmental impact. The novel method not only enhances analytical efficiency but also aligns with green chemistry principles, offering a sustainable solution for routine pharmaceutical analysis.
Asunto(s)
Colorantes Fluorescentes , Tecnología Química Verde , Límite de Detección , Pirroles , Salicilato de Sodio , Espectrometría de Fluorescencia , Sulfonamidas , Sulfonamidas/análisis , Sulfonamidas/química , Espectrometría de Fluorescencia/métodos , Pirroles/química , Tecnología Química Verde/métodos , Colorantes Fluorescentes/química , Salicilato de Sodio/química , Salicilato de Sodio/análisis , Reproducibilidad de los ResultadosRESUMEN
Long-term and excessive use of tetracycline hydrochloride (TC) can lead to its accumulation in the environment, which can cause water contamination, bacterial resistance, and food safety problems. 2,6-Pyridine dicarboxylic acid (DPA) is a major biomarker of Bacillus anthracis spores, and its rapid and sensitive detection is of great significance for disease prevention and counter-terrorism. A bifunctional ratiometric fluorescent nanoprobe has been fabricated to detect DPA and TC. 3,5-dicarboxyphenylboronic acid (BOP) was intercalated into layered europium hydroxide (LEuH) by the ion-exchange method and exfoliated into nanosheets as a fluorescent nanoprobe (PNP). DPA and TC could significantly enhance the red fluorescence of Eu3+ through the antenna effect under different excitation wavelengths, while the fluorescence of BOP can be used as a reference based on the constant emission intensity, realizing ratiometric detection. A low limit of detection (LOD) for the target (DPA: 9.7 nM, TC: 21.9 nM) can be achieved. In addition, visual detection of DPA and TC was realized using color recognition software based on the obvious color changes. This is the first ratiometric fluorescent nanoprobe based on layered rare-earth hydroxide (LRH) for the detection of DPA and TC simultaneously, which opens new ideas in the design of multifunctional probes.
Asunto(s)
Bacillus anthracis , Biomarcadores , Colorantes Fluorescentes , Espectrometría de Fluorescencia , Esporas Bacterianas , Tetraciclina , Colorantes Fluorescentes/química , Espectrometría de Fluorescencia/métodos , Bacillus anthracis/aislamiento & purificación , Biomarcadores/análisis , Tetraciclina/análisis , Límite de Detección , Ácidos Picolínicos/análisis , Carbunco/diagnósticoRESUMEN
Thiols function as antioxidants in food, prolonging shelf life and enhancing flavor. Moreover, thiols are vital biomolecules involved in enzyme activity, cellular signal transduction, and protein folding among critical biological processes. In this paper, the fluorescent probe PYL-NBD was designed and synthesized, which utilized the fluorescent molecule pyrazoline, the lysosome-targeted morpholine moiety, and the sensing moiety NBD. Probe PYL-NBD was tailored for the recognition of biothiols through single-wavelength excitation, yielding distinct fluorescence emission signals: blue for Cys, Hcy, and GSH; green for Cys, Hcy. Probe PYL-NBD exhibited rapid reaction kinetics (<10 min), distinct fluorescence response signals, and low detection limits (15.7 nM for Cys, 14.4 nM for Hcy, and 12.6 nM for GSH). Probe PYL-NBD enabled quantitative determination of Cys content in food samples and L-cysteine capsules. Furthermore, probe PYL-NBD had been successfully applied for confocal imaging with dual-channel detection of biothiols in various biological specimens, including HeLa cells, zebrafish, tumor sections, and Arabidopsis thaliana.
Asunto(s)
Cisteína , Colorantes Fluorescentes , Análisis de los Alimentos , Glutatión , Lisosomas , Espectrometría de Fluorescencia , Pez Cebra , Humanos , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Lisosomas/química , Lisosomas/metabolismo , Células HeLa , Cisteína/análisis , Animales , Análisis de los Alimentos/métodos , Glutatión/análisis , Espectrometría de Fluorescencia/métodos , Homocisteína/análisis , Arabidopsis/química , Límite de Detección , Microscopía ConfocalRESUMEN
Accurate and early detection of atherosclerosis (AS) is imperative for their effective treatment. However, fluorescence probes for efficient diagnosis of AS often encounter insufficient deep tissue penetration, which hinders the reliable assessment of plaque vulnerability. In this work, a reactive oxygen species (ROS) activated near-infrared (NIR) fluorescence and photoacoustic (FL/PA) dual model probe TPA-QO-B is developed by conjugating two chromophores (TPA-QI and O-OH) and ROS-specific group phenylboronic acid ester. The incorporation of ROS-specific group not only induces blue shift in absorbance, but also inhibits the ICT process of TPA-QO-OH, resulting an ignorable initial FL/PA signal. ROS triggers the convertion of TPA-QO-B to TPA-QO-OH, resulting in the concurrent amplification of FL/PA signal. The exceptional selectivity of TPA-QO-B towards ROS makes it effectively distinguish AS mice from the healthy. The NIR emission can achieve a tissue penetration imaging depth of 0.3 cm. Moreover, its PA775 signal possesses the capability to penetrate tissues up to a thickness of 0.8 cm, ensuring deep in vivo imaging of AS model mice in early stage. The ROS-triggered FL/PA dual signal amplification strategy improves the accuracy and addresses the deep tissue penetration problem simultaneously, providing a promising tool for in vivo tracking biomarkers in life science and preclinical applications.
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Colorantes Fluorescentes , Técnicas Fotoacústicas , Placa Aterosclerótica , Especies Reactivas de Oxígeno , Animales , Especies Reactivas de Oxígeno/metabolismo , Técnicas Fotoacústicas/métodos , Placa Aterosclerótica/diagnóstico por imagen , Placa Aterosclerótica/metabolismo , Colorantes Fluorescentes/química , Ratones , Imagen Óptica/métodos , Ratones Endogámicos C57BL , Humanos , MasculinoRESUMEN
In present work, blue carbon dots (b-CDs) were derived from ammonium citrate and guanidine hydrochloride, and red carbon dots (r-CDs) were stemmed from malonate, ethylenediamine and mesotetra (4-carboxyphenyl) porphin based on facile hydrothermal method. Eco-friendly ratiometric fluorescence probe was innovatively constructed to effectively measure Hg2+ utilizing b-CDs and r-CDs. The developed probe displayed two typical emission peaks at 450 nm from b-CDs and 650 nm from r-CDs under the excitation at 360 nm. Mercury ion has strong quenching effect on the fluorescence intensity at 450 nm due to the electron transfer process and the fluorescence change at 450 nm was used as the response signal, whereas the fluorescence intensity at 650 nm kept unchangeable which resulted from the chemical inertness between Hg2+ and r-CDs, serving as the reference signal in the sensing system. Under optimal circumstances, this probe exhibited an excellent linearity between the fluorescence response values of ΔF450/F650 and Hg2+ concentrations over range of 0.01-10 µmol/L, and the limit of detection was down to 5.3 nmol/L. Furthermore, this probe was successfully employed for sensing Hg2+ in practical environmental water samples with satisfied recoveries of 98.5%-105.0%. The constructed ratiometric fluorescent probe provided a rapid, environmental-friendly, reliable, and efficient platform for measuring trace Hg2+ in environmental field.
Asunto(s)
Carbono , Colorantes Fluorescentes , Mercurio , Puntos Cuánticos , Contaminantes Químicos del Agua , Mercurio/análisis , Carbono/química , Colorantes Fluorescentes/química , Contaminantes Químicos del Agua/análisis , Puntos Cuánticos/química , Monitoreo del Ambiente/métodos , Espectrometría de Fluorescencia/métodos , Límite de Detección , FluorescenciaRESUMEN
BACKGROUND: Curcumin has been used in traditional medicine because of its pharmacological activity, including antioxidant, antibacterial, anticancer, and anticarcinogenic properties. Therefore, sensitive and selective monitoring of curcumin is highly demand for practical application. RESULTS: In this study, we describe the construction of a fluorescence method for curcumin assay based on nitrogen-doped MoS2 quantum dots (N-MoS2 QDs). The N-MoS2 QDs are constructed by a solvothermal method using sodium molybdate and Cys as precursors. With the addition of curcumin, the bright blue fluorescence of N-MoS2 QDs is quenched by the inner filter effect (IFE). The QDs emitted bright blue fluorescence and could be quenched by the addition of curcumin via IFE. The dynamic range is the range of 0.1-10 µM for curcumin detection, with a detection limit of 59 nM. N-MoS2 QDs were applied for curcumin assay in real samples with good recovery. In addition, the N-MoS2 QDs exhibited relative low cytotoxicity and could be applied for fluorescence-based imaging in biological samples. SIGNIFICANCE: Our study indicates that the sensor possesses good selectivity to monitor curcumin in water samples, human urine samples, ginger powder samples, mustard samples, and curry samples with satisfactory recoveries. The N-MoS2 QDs possess less cytotoxicity with excellent biocompatibility and were applied for in vitro cell imaging.
Asunto(s)
Curcumina , Disulfuros , Colorantes Fluorescentes , Molibdeno , Nitrógeno , Puntos Cuánticos , Curcumina/química , Curcumina/farmacología , Puntos Cuánticos/química , Molibdeno/química , Humanos , Disulfuros/química , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Nitrógeno/química , Imagen Óptica , Límite de Detección , Espectrometría de Fluorescencia , Supervivencia Celular/efectos de los fármacosRESUMEN
BACKGROUND: Drug-induced liver injury (DILI) is the most important standard for the entrance of clinical drugs into the pharmaceutical market. The elevation of superoxide anion (O2â¢-) during drug metabolism can mediate apoptosis of hepatocytes and further generation of liver damage. Therefore, developing an effective imaging method for evaluating O2â¢- levels during DILI is of great importance. However, current reported O2â¢- fluorescent probes either use short excitation wavelengths or a single intensity detection system, limiting the accurate quantification of O2â¢- in deep tissue in vivo. RESULTS: We developed a NIR-excited ratiometric nanoprobe (CyD-UCNPs) by assembly of O2â¢--sensitive hemicyanine dyes (CyD) on the surface of Tm/Er-codoped upconversion nanoparticles (UCNPs) with the assistance of α-cyclodextrin, which exhibited a robust "turn-on" ratiometric sensing signal. In vitro experiments indicated that CyD-UCNPs respond well to O2â¢- with high selectivity. Furthermore, by taking advantage of the outstanding optical properties produced by the luminescent resonance energy transfer between the UCNPs and CyD upon the excitation of 980 nm, the ratiometric upconversion luminescence signal of CyD-UCNPs was successfully utilized to monitor the fluctuation of O2â¢- levels under phorbol-12-myristate-13-acetate (PMA)/cisplatin-induced oxidative stress in living cells, liver tissues, and zebrafish. More importantly, endogenous change in O2â¢- levels in the liver sites of mice during DILI and its prevention with L-carnitine was visualized using CyD-UCNPs. SIGNIFICANCE: This study provides a ratiometric NIR-excited imaging strategy for investigating the correlation between O2â¢- levels and DILI and its prevention, which is significant for early diagnosis of DILI and preclinical screening of anti-hepatotoxic drugs in vivo.
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Carbocianinas , Enfermedad Hepática Inducida por Sustancias y Drogas , Colorantes Fluorescentes , Rayos Infrarrojos , Nanopartículas , Superóxidos , Enfermedad Hepática Inducida por Sustancias y Drogas/diagnóstico por imagen , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Animales , Superóxidos/análisis , Superóxidos/metabolismo , Superóxidos/química , Ratones , Colorantes Fluorescentes/química , Carbocianinas/química , Nanopartículas/química , Humanos , Pez Cebra , Imagen Óptica , Transducción de Señal/efectos de los fármacosRESUMEN
The capped CdS-ZnS quantum dots (QDs) were synthesized with various thiol capping agents of glycolic acid (TGA), mercaptosuccinic acid (MSA), and L-cysteine (LCY) and used as fluorescence probe for determination of Cu (II) ions. The method of two-level three-factor full-factorial experiment design was used to achieve the best optical fluorescence emission. Results revealed that Cu (II) ions can effectively quench the emission of QDs, and the fluorescence intensity is linearly decreased with increasing Cu (II) ion concentration. The limit of detection for CdS-ZnS@ QDs capped with TGA, MSA, and LCY was obtained at 1.15 × 10-7, 1.32 × 10-7, and 2.19 × 10-7 mol L-1, respectively, with linear dynamic range of 3.13 × 10-6 to 1.41 × 10-4 mol L-1. Luminescence quantum yields of CdS-ZnS@LCY, CdS-ZnS@MSA, and CdS-ZnS@TGA were obtained at 4.17, 1.92, and 2.47, respectively. Results indicated that no significant quenching occurred in the presence of the other metal ions. The binding constant (Kb) of capped CdS-ZnS@ QDs with Cu2+ and the other metal ions was also investigated and discussed. The Kb value for Cu2+ was obtained considerably more than that the other ions. This work presents a new and sensitive method for determination of Cu2+ ion.
Asunto(s)
Compuestos de Cadmio , Cobre , Colorantes Fluorescentes , Puntos Cuánticos , Compuestos de Sulfhidrilo , Sulfuros , Propiedades de Superficie , Compuestos de Zinc , Puntos Cuánticos/química , Cobre/química , Cobre/análisis , Sulfuros/química , Compuestos de Zinc/química , Compuestos de Cadmio/química , Compuestos de Sulfhidrilo/química , Compuestos de Sulfhidrilo/análisis , Colorantes Fluorescentes/química , Colorantes Fluorescentes/síntesis química , Espectrometría de Fluorescencia , Fluorescencia , Iones/química , Iones/análisisRESUMEN
A highly water-soluble and fluorescent N,S-doped carbon dots/europium (N,S-CDs/Eu) was successfully synthesized via a secondary hydrothermal method. This involved surface modification of N,S-CDs derived from sunflower stem pith (SSP) with europium ions (Eu3+) doping. When excited within the range of 400-470 nm, N,S-CDs/Eu exhibited a stable and broad optimal emission wavelength ranging from 505 to 540 nm. Notably, the photoluminescence quantum yield (PLQY) of N,S-CDs/Eu is 31.4%, significantly higher than the 19.5% observed for N,S-CDs. Additionally, by dissolving N,S-CDs/Eu into polyvinyl alcohol (PVA), a uniform fluorescent anti-counterfeiting ink can be prepared. The N,S-CDs/Eu/TiO2 composite demonstrates excellent photocatalytic degradation ability towards the organic dye methylene blue (MB). N,S-CDs/Eu has potential in the field of fluorescent inks and photocatalysis due to its simple and efficient preparation and excellent properties.