RESUMEN

Many Clostridium species are found as commensal members of the intestinal microbiota. However, imbalances of the microbiota may lead to certain infections caused by these microorganisms, mainly Clostridium butyricum, Clostridium difficile, and Clostridium perfringens. In many cases, infection recurrence can occur after antibiotics, indicating the need for novel therapeutic options that act on the pathogens and also restore the microbiota. Herein, the in vitro antimicrobial activity and probiotic potential of clinical and reference strains of Bifidobacterium and Lactobacillus were investigated against Clostridium species. Antimicrobial activity was evaluated by the agar spot test and inhibition of gas production. Then, the probiotic potential of selected strains was assessed by analyzing their coaggregation ability, adhesive properties to host cells and mucin, tolerance to acidic pH and bile salts, and antimicrobial susceptibility profiles. Lactobacillus plantarum ATCC 8014 was the most promising strain based on its inhibitory activity against Clostridium spp. Also, this strain met criteria to be considered a probiotic based on its coaggregation ability, adhesive properties, and tolerance to harsh pH and bile acid salt conditions. The results indicate that among the studied strains, L. plantarum ATCC 8014 presents probiotic potential for controlling infections induced by the studied Clostridium species and should be further evaluated in in vivo animal models.

Asunto(s)

Bifidobacterium/crecimiento & desarrollo , Infecciones por Clostridium/microbiología , Clostridium/crecimiento & desarrollo , Microbioma Gastrointestinal , Lactobacillus/crecimiento & desarrollo , Interacciones Microbianas , Probióticos , Antiinfecciosos , Adhesión Bacteriana , Ácidos y Sales Biliares , Infecciones por Clostridium/tratamiento farmacológico , Clostridium butyricum/crecimiento & desarrollo , Clostridium perfringens/crecimiento & desarrollo , Humanos , Concentración de Iones de Hidrógeno , Lactobacillus plantarum/crecimiento & desarrollo , Pruebas de Sensibilidad Microbiana , Probióticos/uso terapéutico

RESUMEN

Abstract Despite recent advances in food production technology, food-borne diseases (FBD) remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil), Rosmarinus officinalis L. (rosemary), Origanum majorana L. (marjoram), Mentha × piperita L. var. Piperita (peppermint), Thymus vulgaris L. (thyme) and Pimpinella anisum L. (anise) against C. perfringens strain A. Chemical compositions of the oils were determined by GC–MS (gas chromatography–mass spectrometry). The identities of the isolated compounds were established from the respective Kováts indices, and a comparison of mass spectral data was made with those reported earlier. The antibacterial activity was assessed from minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. Minimum inhibitory concentration values were 1.25 mg mL-1 for thyme, 5.0 mg mL-1 for basil and marjoram, and 10 mg mL-1 for rosemary, peppermint and anise. All oils showed bactericidal activity at their minimum inhibitory concentration, except anise oil, which was only bacteriostatic. The use of essential oils from these common spices might serve as an alternative to the use of chemical preservatives in the control and inactivation of pathogens in commercially produced food systems.

Asunto(s)

Aceites de Plantas/farmacología , Aceites Volátiles/farmacología , Clostridium perfringens/efectos de los fármacos , Antibacterianos/farmacología , Aceites de Plantas/análisis , Brasil , Aceites Volátiles/análisis , Pruebas de Sensibilidad Microbiana , Clostridium perfringens/crecimiento & desarrollo , Ocimum basilicum/química , Rosmarinus/química , Origanum/química , Thymus (Planta)/química , Cromatografía de Gases y Espectrometría de Masas , Antibacterianos/análisis

RESUMEN

Despite recent advances in food production technology, food-borne diseases (FBD) remain a challenging public health concern. In several countries, including Brazil, Clostridium perfringens is among the five main causative agents of food-borne diseases. The present study determines antimicrobial activities of essential oils of six condiments commonly used in Brazil, viz., Ocimum basilicum L. (basil), Rosmarinus officinalis L. (rosemary), Origanum majorana L. (marjoram), Mentha × piperita L. var. Piperita (peppermint), Thymus vulgaris L. (thyme) and Pimpinella anisum L. (anise) against C. perfringens strain A. Chemical compositions of the oils were determined by GC-MS (gas chromatography-mass spectrometry). The identities of the isolated compounds were established from the respective Kováts indices, and a comparison of mass spectral data was made with those reported earlier. The antibacterial activity was assessed from minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. Minimum inhibitory concentration values were 1.25mgmL(-1) for thyme, 5.0mgmL(-1) for basil and marjoram, and 10mgmL(-1) for rosemary, peppermint and anise. All oils showed bactericidal activity at their minimum inhibitory concentration, except anise oil, which was only bacteriostatic. The use of essential oils from these common spices might serve as an alternative to the use of chemical preservatives in the control and inactivation of pathogens in commercially produced food systems.

Asunto(s)

Antibacterianos/farmacología , Clostridium perfringens/efectos de los fármacos , Aceites Volátiles/farmacología , Aceites de Plantas/farmacología , Antibacterianos/análisis , Brasil , Clostridium perfringens/crecimiento & desarrollo , Cromatografía de Gases y Espectrometría de Masas , Pruebas de Sensibilidad Microbiana , Ocimum basilicum/química , Aceites Volátiles/análisis , Origanum/química , Aceites de Plantas/análisis , Rosmarinus/química , Thymus (Planta)/química

RESUMEN

Visando estudar a presença de Clostridium perfringens em frangos de cortes provenientes de aviários da região de Ribeirão Preto-SP, amostras de conteúdo cecal de aves foram pesquisadas quanto a presença desse microrganismo. As amostras foram semeadas em meios seletivos para clostrídios e incubadas anaerobica­mente. As culturas foram identificadas e caracterizadas pelo método de Gram e séries bioquímicas. Posteriormente realizou-se teste de inoculação em camundongos para confirmar patogenicidade. De um total de 560 amostras coletadas, 374(66,78%) foram positivas para o gênero Clostridium, sendo 94 (16,78 %) Clostridium perfringens. Verificou–se que 19 (3,4%) amostras foram positivas para outras espécies de clostrídios patogênicos como Clostridium chauvoei (3 - 0,54 %), Clostridium sordelli (9 - 1,61%), Clostridium bifermentans (3 - 0,54%), Clostridium septicum (3 - 0,54%) e Clostridium tetani (1 - 0,18%).

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The aim of the present study was to detect Clostridium perfringens in broiler chickens from intensive poultry farms in Ribeirão Preto-SP. We collected 516 samples of caecal content that were cultivated onto selective medium for Clostridium and incubated under anaerobic conditions. Gram smears were carried out from the positive samples and identified using biochemical tests. Mice inoculation test was performed in order to confirm pathogenicity. The results showed that 66.78% of the samples were positive for Clostridia, of which 16.78% were C. perfringens. It was confirmed that 19 (3.4%) of the samples were positive to other Clostridia pathogenic species such as Clostridium chauvoei (3 - 0.54%), Clostridium sordelli (9 - 1.61%), Clostridium bifermentans (3 - 0.54%), Clostridium septicum (3 - 0.54%) and Clostridium tetani (1 - 0.18%).

Asunto(s)

Animales , Clostridium perfringens/crecimiento & desarrollo , Clostridium perfringens/patogenicidad

RESUMEN

Visando estudar a presença de Clostridium perfringens em frangos de cortes provenientes de aviários da região de Ribeirão Preto-SP, amostras de conteúdo cecal de aves foram pesquisadas quanto a presença desse microrganismo. As amostras foram semeadas em meios seletivos para clostrídios e incubadas anaerobica­mente. As culturas foram identificadas e caracterizadas pelo método de Gram e séries bioquímicas. Posteriormente realizou-se teste de inoculação em camundongos para confirmar patogenicidade. De um total de 560 amostras coletadas, 374(66,78%) foram positivas para o gênero Clostridium, sendo 94 (16,78 %) Clostridium perfringens. Verificou–se que 19 (3,4%) amostras foram positivas para outras espécies de clostrídios patogênicos como Clostridium chauvoei (3 - 0,54 %), Clostridium sordelli (9 - 1,61%), Clostridium bifermentans (3 - 0,54%), Clostridium septicum (3 - 0,54%) e Clostridium tetani (1 - 0,18%).(AU)

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The aim of the present study was to detect Clostridium perfringens in broiler chickens from intensive poultry farms in Ribeirão Preto-SP. We collected 516 samples of caecal content that were cultivated onto selective medium for Clostridium and incubated under anaerobic conditions. Gram smears were carried out from the positive samples and identified using biochemical tests. Mice inoculation test was performed in order to confirm pathogenicity. The results showed that 66.78% of the samples were positive for Clostridia, of which 16.78% were C. perfringens. It was confirmed that 19 (3.4%) of the samples were positive to other Clostridia pathogenic species such as Clostridium chauvoei (3 - 0.54%), Clostridium sordelli (9 - 1.61%), Clostridium bifermentans (3 - 0.54%), Clostridium septicum (3 - 0.54%) and Clostridium tetani (1 - 0.18%).(AU)

Asunto(s)

Animales , Clostridium perfringens/crecimiento & desarrollo , Clostridium perfringens/patogenicidad

RESUMEN

The minimum inhibitory concentration (MIC) was determined for 13 antibiotics against Clostridium perfringens isolated from Brazilian piglets. The collection of isolates was performed in June to October 2010. All isolates were susceptible to amoxicillin and ceftiofur, whereas most were resistant to tetracycline and lincomycin. Avilamycin and narasin were more effective against isolates from non-diarrheic than from diarrheic piglets. The other antimicrobials were less active in need of high concentrations to inhibit the growth of the C. perfringens type A. These results suggest the need for further studies evaluating molecular factors related to the antimicrobial resistance of C. perfringens.

Asunto(s)

Animales , Antibacterianos , Amoxicilina/aislamiento & purificación , Clostridium perfringens/crecimiento & desarrollo , Clostridium perfringens/aislamiento & purificación , Diarrea , Susceptibilidad a Enfermedades , Resistencia a la Tetraciclina , Métodos , Porcinos

RESUMEN

Three microorganisms were assayed to evaluate the microbiological quality in the seawater at a resort on the Mexican Pacific coast, and to test for possible associations among the titers of the various bacteria, their possible correlations with environmental conditions, and with the location of potential wastewater outflows. Significant microorganism levels were found (at Caletilla beach, Hornos beach, and Papagayo beach, respectively: for Enterococcus 157, 153, and 149, for C. perfringens 35, 89, and 56, for S. aureus 244,137, and 279CFU/100ml), often in excess of the presently set guideline values. In general, bacterial titers were higher during rainy season than in dry season. For S. aureus, in both seasons, highest concentrations were found at 3pm, the time of highest tourist presence at the beaches. Our results argue for the use of these three microorganisms as part of a set of indicators in the routine microbiological evaluation of Mexican beachwaters.

Asunto(s)

Playas/estadística & datos numéricos , Clostridium perfringens/crecimiento & desarrollo , Enterococcus/crecimiento & desarrollo , Agua de Mar/microbiología , Staphylococcus aureus/crecimiento & desarrollo , Microbiología del Agua , Calidad del Agua/normas , Playas/normas , México , Contaminación Química del Agua/estadística & datos numéricos

RESUMEN

Vegetable tannins are water-soluble polyphenolic compounds of varying molecular weights that occur abundantly in nature. The diet of many free-ranging wild animals contains significant amounts of tannins. Also, commercial tannins are used in animal industry as food additives to improve animal performance. In order to further determine the capacity of tannins to inhibit the development of intestinal diseases produced by Clostridium pefringens, we evaluated here the effect of tannins from quebracho, chestnut or combinations of both on C. perfringens and their toxins. The C. perfringens (types A, B, C, D and E) growth obtained from the intestine of healthy and diseased animals was reduced in a dose-dependent manner in the presence of quebracho tannins, chestnut tannins, combinations of both or a commercial formula based in these tannins. Although the minimal inhibitory concentration of both tannins varied between isolates, no statistically significant differences were observed between isolates from healthy or sick animals. Comparative analysis showed that the concentrations of quebracho tannin inhibiting the growth of C. perfringens were higher than chestnut tannin. In fact, antibacterial effect of quebracho tannin was increased up to 20 times with the addition of 25% of chestnut tannin and 85 times with 75% of chestnut tannin. Antibacterial activity of the commercial product was up to ~50 times higher than quebracho tannin alone. Quebracho tannin showed partial bactericidal activity, whereas chestnut tannin activity was stronger. Both tannins were able to reduce the alpha toxin lecithinase activity and epsilon toxin cytotoxicity in MDCK cells. These results suggest that tannin-supplemented diet could be useful to prevent some clostridial diseases.

Asunto(s)

Toxinas Bacterianas/metabolismo , Infecciones por Clostridium/veterinaria , Clostridium perfringens/efectos de los fármacos , Enfermedades Intestinales/veterinaria , Taninos/farmacología , Animales , Línea Celular , Infecciones por Clostridium/microbiología , Infecciones por Clostridium/prevención & control , Clostridium perfringens/crecimiento & desarrollo , Perros , Concentración 50 Inhibidora , Enfermedades Intestinales/microbiología , Enfermedades Intestinales/prevención & control , Pruebas de Sensibilidad Microbiana/veterinaria , Estadísticas no Paramétricas

RESUMEN

Histopathological analysis of 108 intestine samples (103 grossly affected ileum and 5 jejunum) taken from Clostridium-induced neonatal alpaca (Vicugna pacos) enterotoxemia mortalities collected in the Departments of Arequipa, Puno and Cusco of southern Peru during the 2005-2008 birth seasons (January-March), revealed the presence of large numbers of both asexual and sexual stages of Eimeria macusaniensis in 33/108 (30.55%) of the samples with moderate to severe necrotized and/or hemorrhagic enteritis. It is proposed that damage to the mucosa produced by coccidial infections may facilitate overgrowth of Clostridium perfringens with toxin production leading to fatal enterotoxemia.

Asunto(s)

Animales Recién Nacidos , Camélidos del Nuevo Mundo , Coccidiosis/veterinaria , Eimeria/fisiología , Enterotoxemia/complicaciones , Enterotoxemia/patología , Animales , Clostridium perfringens/crecimiento & desarrollo , Coccidiosis/complicaciones , Coccidiosis/patología , Contenido Digestivo/química , Contenido Digestivo/microbiología , Contenido Digestivo/parasitología

RESUMEN

The combined effect of Citricidal wih irradiation on Clostridium perfringens growth from spores in a sous-vide processed marinated pork meat Mexican entrée was investigated. Citricidal was added at 200 or 800 ppm after mixing pork meat with tomatillo sauce and inoculated with 3 log(10) CFU/g of C. perfringens spores. Samples were irradiated at either 0 or 2 kGy, heated to an internal temperature of 71 degrees C, and stored at 4 degrees C for 28 d, 15 degrees C for 45 d, and 25 degrees C for 26 h. To simulate the conditions that may occur during transportation, distribution, storage, or handling in supermarkets or by consumers, the effect of static temperature abuse on C. perfringens growth was assessed by transferring samples stored at 4 to 25 degrees C for 13 and 15 h. Total C. perfringens populations were determined by plating diluted samples on tryptose-sulfite-cycloserine agar. Growth was not observed up to 45 d of storage at 15 degrees C in samples supplemented with 800 ppm of Citricidal. At 25 degrees C, no significant differences (P > 0.05) on the lag phase duration due to antimicrobial treatments was observed. The temperature abuse of refrigerated products for up to 15 h did not lead to C. perfringens growth to high infective dose levels of 1 million cells required to cause food poisoning. The results suggest that 800 ppm Citricidal can have significant bacteriostatic activity against C. perfringens and may provide a degree of protection against this pathogen in sous-vide processed marinated pork meat Mexican entrée, under mild temperature abuse (

Asunto(s)

Clostridium perfringens/crecimiento & desarrollo , Manipulación de Alimentos/métodos , Carne/microbiología , Esporas Bacterianas/crecimiento & desarrollo , Animales , Antibacterianos/administración & dosificación , Citrus paradisi/química , Irradiación de Alimentos , Frutas/química , Calor , México/etnología , Extractos Vegetales/administración & dosificación , Semillas/química , Porcinos , Vacio

RESUMEN

In order to reduce the toxicity of Clostridium perfringens fermentation broths used in vaccine preparation, we developed two-phase aqueous systems for removal of toxin-activating proteases. Removal of the proteases inhibits the conversion of protoxin to active toxin. In order to establish the conditions under which the phase separation occurs, binodal curves, formed by poly(ethylene glycol) (PEG) and sodium citrate, were investigated at different values of pH and PEG molar mass. A 2(4)-experimental design was used to evaluate the influence of PEG molar mass and concentration, citrate concentration and pH on protease partition coefficient, removal.factor and protease removal yield. It has been found that simultaneous increase in PEG molar mass and decrease in citrate concentration remarkably improved the removal factor, whereas the protease removal yield showed an opposite trend. The best conditions for the system under consideration (removal factor of 2.69 and yield of 116%) were obtained at pH 8.0 using PEG molar mass of 8000 g mol(-1) and concentrations of PEG and citrate of 24 and 15%, respectively.

Asunto(s)

Toxinas Bacterianas/aislamiento & purificación , Técnicas de Química Analítica/métodos , Clostridium perfringens/enzimología , Péptido Hidrolasas/aislamiento & purificación , Transición de Fase , Proteínas de Unión al Calcio/aislamiento & purificación , Citratos , Clostridium perfringens/crecimiento & desarrollo , Clostridium perfringens/metabolismo , Medios de Cultivo , Fermentación , Concentración de Iones de Hidrógeno , Péptido Hidrolasas/metabolismo , Polietilenglicoles , Citrato de Sodio

RESUMEN

The pathological findings in sheep with peracute experimental Clostridium perfringens type D enterotoxemia are described. Of 16 animals inoculated intraduodenally with a whole culture of this microorganism and a starch solution in the abomasum, 12 developed clinical signs including increased respiratory efforts, recumbency, paddling, bleating, convulsions, blindness, and opisthotonus. Diarrhea was not observed in any of the animals. The time lapse between the beginning of intraduodenal infusion and onset of clinical signs varied between 30 minutes and 26 hours, and the clinical course varied between 1 and 9 hours. Gross postmortem changes were observed in these 12 animals and included pulmonary edema; excess pericardial, peritoneal, or pleural fluid with or without strands of fibrin; liquid small intestinal contents; leptomeningeal edema; cerebellar coning; and subcapsular petechiae on kidneys. Histological changes consisted of severe edema of pleura and interlobular septa and around blood vessels and airways and acidophilic, homogeneous, proteinaceous perivascular edema in the brain. Five of 12 animals (42%) with clinical signs consistent with enterotoxemia lacked specific histological lesions in the brain. None of the intoxicated or control animals developed nephrosis. Glucose was detected in the urine of 3 of 6 animals that were tested for this analyte. These results stress the importance of the use of histological examination of the brain, coupled with epsilon toxin detection, for a definitive diagnosis of C. perfringens type D enterotoxemia in sheep.

Asunto(s)

Infecciones por Clostridium/patología , Infecciones por Clostridium/veterinaria , Clostridium perfringens/crecimiento & desarrollo , Enterotoxemia/microbiología , Enterotoxemia/patología , Enfermedades de las Ovejas/microbiología , Enfermedades de las Ovejas/patología , Animales , Toxinas Bacterianas/sangre , Encéfalo/microbiología , Encéfalo/patología , Infecciones por Clostridium/microbiología , Infecciones por Clostridium/orina , Enterotoxemia/orina , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Tracto Gastrointestinal/microbiología , Tracto Gastrointestinal/patología , Glucosuria/veterinaria , Histocitoquímica/veterinaria , Riñón/microbiología , Riñón/patología , Pulmón/microbiología , Pulmón/patología , Masculino , Ratones , Pruebas de Neutralización , Distribución Aleatoria , Ovinos

RESUMEN

Due to the current tendency of cooking and heating meat prepared foods in microwave ovens and the possibility that they transmit bacterial diseases, the survival rate of spore-forming bacteria was evaluated in minced meat samples. Meat was innoculated with a known number of Bacillus cereus and Clostridium perfringens spores, and laterly thawed and cooked in an Amana microwave oven (2450 Hz). Survival rate was determined according to the methodology described by Vanderzant & Splittstoesser, and the activity of the enzyme acid phosphatase was determined as cooking parameter. B. cereus spore showed a decrease in its number as the time of exposition increased, but without fully disappearing. C. perfringens spores also decreased in number, but showed a later increase, associated with the germination of survival spores.

Asunto(s)

Bacillus cereus/efectos de la radiación , Clostridium perfringens/efectos de la radiación , Carne/microbiología , Microondas , Bacillus cereus/crecimiento & desarrollo , Clostridium perfringens/crecimiento & desarrollo , Factores de Tiempo

RESUMEN

Debido a la creciente tendencia de cocinar y recalentar en horno de microondas platillos a base de carne y a la posibilidad de que estos transmitan enfermedades de origen bacteriano, se evaluó la capacidad de sobrevivencia de esporas de bacterias inoculadas en carne molida y tratadas en horno de microonda. Las tortas de carne fueron inoculadas con un número conocido de esporas de Bacillus cereus o Clostridium perfringens y posteriormente cocinadas en horno de microondas Amana de 2450 Hz. Se utilizó la técnica descrita por Vanderzant y Splittstoesser para determinar la tase de sobrevivencia y la actividad de la enxima fosfatasa ácida como parámetro de conocimiento. Las esporas de B. cereus mostraron una tendencia a disminuir en número, conforme aumenta el tiempo de exposición a la radiación, pero si llegar a desaparecer totalmente. Las esporas de C. perfringens disminuyen en número al aumentar el tiempo de exposición, pero presentan un segundo aumento asociado a la germinación de esporas sobrevivientes.

Asunto(s)

Bacillus cereus/efectos de la radiación , Clostridium perfringens/efectos de la radiación , Carne/microbiología , Microondas , Bacillus cereus/crecimiento & desarrollo , Clostridium perfringens/crecimiento & desarrollo , Factores de Tiempo

RESUMEN

A refractile inclusion body produced by vegetative cells of Clostridium perfringens at temperatures above 40 degrees C was isolated and partially characterized. The inclusion was composed of protein and could be solubilized by sodium dodecyl sulphate plus either dithiothreitol or beta-mercaptoethanol. The solubilized inclusion showed no antigenic relationship with Cl. perfringens enterotoxin. One major band with an apparent MW of 47 kDa was demonstrated after polyacrylamide gel electrophoresis of the solubilized inclusion. Both enterotoxin-positive and enterotoxin-negative strains produced the inclusion body. No effect on the morphology of several eucaryotic cell lines was observed when solubilized or intact inclusion was added to the cell cultures.

Asunto(s)

Proteínas Bacterianas/aislamiento & purificación , Clostridium perfringens/ultraestructura , Cuerpos de Inclusión/química , Animales , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/farmacología , Bovinos , Línea Celular , Pollos , Chlorocebus aethiops , Clostridium perfringens/química , Clostridium perfringens/crecimiento & desarrollo , Humanos , Cuerpos de Inclusión/ultraestructura , Ratones , Peso Molecular

RESUMEN

The effect of human bile juice and bile salts (sodium cholate, sodium taurocholate, sodium glycochenodeoxycholate and sodium chenodeoxycholate) on growth, sporulation and enterotoxin production by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens was determined. Each bile salt inhibited growth to a different degree. A mixture of bile salts completely inhibited the growth of enterotoxin-positive strains of this organism. Human bile juice completely inhibited the growth of all the strains at a dilution of 1:320. A distinct stimulatory effect of the bile salts on sporulation was observed in the case of C. perfringens strains NCTC 8239 and NCTC 8679. The salts also increased enterotoxin concentrations in the cell extracts of the enterotoxin-positive strains tested. No effect on enterotoxin production was detected when an enterotoxin-negative strain was examined.

Asunto(s)

Ácidos y Sales Biliares/farmacología , Clostridium perfringens/crecimiento & desarrollo , Enterotoxinas/biosíntesis , Clostridium perfringens/metabolismo , Clostridium perfringens/fisiología , Humanos , Cinética , Esporas Bacterianas

RESUMEN

Con la finalidad de probar el rendimiento de dos caldos de cultivo, usados comunmente para anaerobios, caldo tiaglicolato y caldo schedle. Se evaluaron ambos caldos con una cepa de clostridium perfringens, estableciéndose para dicho fin, curvas de crecimiento a temperaturas óptimas de crecimiento (37ºC y 46ºC) para este microorganismo. En el presente trabajo, no se encontraron diferencias significativas en cuanto al rendimiento de ambos caldos. Por lo que se concluye que, tanto en caldo tiaglicolato, usado para el aislamiento de clostridium perfringens en alimentos, y caldo Schedler, se obtuvieron buenos rendimientos.

Asunto(s)

Clostridium perfringens/clasificación , Clostridium perfringens/crecimiento & desarrollo , Tioglicolatos , Medios de Cultivo/análisis , Medios de Cultivo/aislamiento & purificación

RESUMEN

This work was undertaken to study the use of molasses and cheese whey in culture media for growth and toxigenic activities of C. perfringens type D. Three media were used namely: 1) A modified Gordon medium 2) a molasses medium and 3) a whey medium which composition are shown in Table 1. The experiments were carried out in a 400 ml microfermentor by using 300 ml of media with automatic pH control (7,0 +/- 0,1) at 37 degrees C. Samples were taken for determination of microbial count and toxigenic activity. Fig. 1, 2, and 3 show the results obtained. The toxigenic activities obtained with the molasses and the Gordon media are similar, while it was not detected any activity in the cheese whey medium. It can be concluded that molasses could be a suitable and inexpensive basic component of culture media for toxin production by C. perfringens type D.

Asunto(s)

Toxinas Bacterianas/metabolismo , Clostridium perfringens/crecimiento & desarrollo , Medios de Cultivo , Lactosa , Melaza , Técnicas Bacteriológicas , Clostridium perfringens/metabolismo