RESUMEN
In mammals, long bones are formed by ossification of a cartilaginous mould during early stages of development, through the formation of structures called the primary ossification centre, the secondary ossification centres (SOCs) and the physeal cartilages (PCs). The PC is responsible for long bone growth. The morphology of the PC and the SOCs varies during different stages of femoral growth. In this respect, several details involving the process of murine femoral development are lacking. In the present study, a morphological characterization of femur development from the embryonic period to adulthood in mice was studied using micro-computed tomography (micro-CT). To achieve this aim, femora were collected at embryonic day (E) 14.5, E16.5 and E18.5 and at postnatal day (P)1, P7, P14, P35, P46 and P52. CT images were obtained using a micro-CT scanner (X-SkyScan 1172; Micro Photonics) and analysed using the micro-CT 3D visualization software Mimics (Materialise NV, Leuven, Belgium) and NRecon (Micro Photonics). The results of the present study revealed that the femur and its PCs and SOCs undergo morphological changes during different stages of development, including changes in their shape as well as position and thickness. These changes may be due to the response of the femur to mechanical loads imposed by muscle surrounding the bone during these stages of development. The result of the present study is important to improve our knowledge related to ossification and growth patterns of mouse femur during development.
Asunto(s)
Desarrollo Óseo/fisiología , Cartílago/fisiología , Embrión de Mamíferos/fisiología , Desarrollo Embrionario/fisiología , Miembro Posterior/diagnóstico por imagen , Microtomografía por Rayos X/métodos , Animales , RatonesRESUMEN
Long bone formation starts early during embryonic development through a process known as endochondral ossification. This is a highly regulated mechanism that involves several mechanical and biochemical factors. Because long bone development is an extremely complex process, it is unclear how biochemical regulation is affected when dynamic loads are applied, and also how the combination of mechanical and biochemical factors affect the shape acquired by the bone during early development. In this study, we develop a mechanobiological model combining: (1) a reaction-diffusion system to describe the biochemical process and (2) a poroelastic model to determine the stresses and fluid flow due to loading. We simulate endochondral ossification and the change in long bone shapes during embryonic stages. The mathematical model is based on a multiscale framework, which consisted in computing the evolution of the negative feedback loop between Ihh/PTHrP and the diffusion of VEGF molecule (on the order of days) and dynamic loading (on the order of seconds). We compare our morphological predictions with the femurs of embryonic mice. The results obtained from the model demonstrate that pattern formation of Ihh, PTHrP and VEGF predict the development of the main structures within long bones such as the primary ossification center, the bone collar, the growth fronts and the cartilaginous epiphysis. Additionally, our results suggest high load pressures and frequencies alter biochemical diffusion and cartilage formation. Our model incorporates the biochemical and mechanical stimuli and their interaction that influence endochondral ossification during embryonic growth. The mechanobiochemical framework allows us to probe the effects of molecular events and mechanical loading on development of bone.
Asunto(s)
Biofisica , Simulación por Computador , Modelos Biológicos , Osteogénesis , Animales , Cartílago/fisiología , Fémur/anatomía & histología , Análisis de Elementos Finitos , Placa de Crecimiento/crecimiento & desarrollo , Proteínas Hedgehog/metabolismo , Ratones Endogámicos BALB C , Morfogénesis , Proteína Relacionada con la Hormona Paratiroidea/metabolismo , Reología , Estrés MecánicoRESUMEN
The aim of this study was to investigate the abilities of cartilage-derived morphogenetic protein 1 (CDMP1) transgenic cell sheets in repairing rabbit cartilage defects. Rabbit CDMP1 transgenic bone marrow mesenchymal stem cell (BMSC) sheets (CDMP1-BMSCs) were cultured on temperature-sensitive culture dishes, and CDMP1 expression and type II collagen protein in the cell sheets were detected. Tissue-engineered cell sheets were constructed and transplanted into defect rabbit thyroid cartilage, to investigate the expression of engineered cartilage collagen protein and proteoglycan (GAG). The experiment was divided into three groups; A) BMSC sheet, B) Ad-CMV-eGFP-transfected cell sheet, and C) Ad-CMV-hCDMP1-IRES-eGFP-transfected cell sheet. The expression of CDMP1 was detected in the transgenic cell sheets. The engineered cartilage exhibited positive immunohistochemical and Alcian blue staining. The expression levels of type II collagen protein and GAG in group A were positive, whereas those in group B and group C were negative (P < 0.05). The CDMP1-BMSC sheets had a good cartilage differentiation activity, and could effectively repair rabbit laryngeal cartilage defects.
Asunto(s)
Cartílago/fisiología , Factor 5 de Diferenciación de Crecimiento/genética , Trasplante de Células Madre Mesenquimatosas , Regeneración , Ingeniería de Tejidos , Andamios del Tejido/química , Animales , Cartílago/citología , Células Cultivadas , Femenino , Factor 5 de Diferenciación de Crecimiento/metabolismo , Masculino , Células Madre Mesenquimatosas/metabolismo , Conejos , Andamios del Tejido/efectos adversosRESUMEN
The Mexican Axolotl is one of the few tetrapod species that is capable of regenerating complete skeletal elements in injured adult limbs. Whether the skeleton (bone and cartilage) plays a role in the patterning and contribution to the skeletal regenerate is currently unresolved. We tested the induction of pattern formation, the effect on cell proliferation, and contributions of skeletal tissues (cartilage, bone, and periosteum) to the regenerating axolotl limb. We found that bone tissue grafts from transgenic donors expressing GFP fail to induce pattern formation and do not contribute to the newly regenerated skeleton. Periosteum tissue grafts, on the other hand, have both of these activities. These observations reveal that skeletal tissue does not contribute to the regeneration of skeletal elements; rather, these structures are patterned by and derived from cells of non-skeletal connective tissue origin.
Asunto(s)
Huesos/fisiología , Cartílago/fisiología , Regeneración/fisiología , Ambystoma mexicanum , Animales , Células del Tejido Conectivo/fisiología , Extremidades , Periostio/citología , Periostio/fisiologíaRESUMEN
As banked human tissues are not widely available, the development of new non-destructive and contactless techniques to evaluate the quality of allografts before distribution for transplantation is very important. Also, tissues will be processed accordingly to standard procedures and to minimize disease transmission most tissue banks will include a decontamination or sterilization step such as ionizing radiation. In this work, we present a new method to evaluate the internal structure of frozen or glycerol-processed human cartilages, submitted to various dosis of irradiation, using the total optical attenuation coefficient retrieved from optical coherence tomography (OCT) images. Our results show a close relationship between tensile properties and the total optical attenuation coefficient of cartilages. Therefore, OCT associated with the total optical attenuation coefficient open a new window to evaluate quantitatively biological changes in processed tissues.
Asunto(s)
Cartílago/fisiología , Radiación Ionizante , Tomografía de Coherencia Óptica/métodos , Cartílago/efectos de la radiación , HumanosRESUMEN
From early dinosaurs with as many as nine wrist bones, modern birds evolved to develop only four ossifications. Their identity is uncertain, with different labels used in palaeontology and developmental biology. We examined embryos of several species and studied chicken embryos in detail through a new technique allowing whole-mount immunofluorescence of the embryonic cartilaginous skeleton. Beyond previous controversy, we establish that the proximal-anterior ossification develops from a composite radiale+intermedium cartilage, consistent with fusion of radiale and intermedium observed in some theropod dinosaurs. Despite previous claims that the development of the distal-anterior ossification does not support the dinosaur-bird link, we found its embryonic precursor shows two distinct regions of both collagen type II and collagen type IX expression, resembling the composite semilunate bone of bird-like dinosaurs (distal carpal 1+distal carpal 2). The distal-posterior ossification develops from a cartilage referred to as "element x," but its position corresponds to distal carpal 3. The proximal-posterior ossification is perhaps most controversial: It is labelled as the ulnare in palaeontology, but we confirm the embryonic ulnare is lost during development. Re-examination of the fossil evidence reveals the ulnare was actually absent in bird-like dinosaurs. We confirm the proximal-posterior bone is a pisiform in terms of embryonic position and its development as a sesamoid associated to a tendon. However, the pisiform is absent in bird-like dinosaurs, which are known from several articulated specimens. The combined data provide compelling evidence of a remarkable evolutionary reversal: A large, ossified pisiform re-evolved in the lineage leading to birds, after a period in which it was either absent, nonossified, or very small, consistently escaping fossil preservation. The bird wrist provides a modern example of how developmental and paleontological data illuminate each other. Based on all available data, we introduce a new nomenclature for bird wrist ossifications.
Asunto(s)
Evolución Biológica , Carpo Animal/anatomía & histología , Embrión de Pollo/anatomía & histología , Dinosaurios/anatomía & histología , Animales , Carpo Animal/metabolismo , Cartílago/anatomía & histología , Cartílago/fisiología , Embrión de Pollo/metabolismo , Colágeno Tipo II/genética , Colágeno Tipo II/metabolismo , Colágeno Tipo IX/genética , Colágeno Tipo IX/metabolismo , Dinosaurios/clasificación , Dinosaurios/fisiología , Fósiles , Expresión Génica , Paleontología , Tendones/anatomía & histología , Tendones/fisiología , Alas de Animales/anatomía & histología , Alas de Animales/fisiologíaRESUMEN
Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-based scaffolds in tissue engineering. Advances in the preparation, modification, and application of PHBHHx scaffolds are discussed.
Asunto(s)
Ácido 3-Hidroxibutírico/química , Materiales Biocompatibles/química , Caproatos/química , Ingeniería de Tejidos/métodos , Andamios del Tejido/química , Ácido 3-Hidroxibutírico/uso terapéutico , Materiales Biocompatibles/uso terapéutico , Huesos/fisiología , Caproatos/uso terapéutico , Cartílago/fisiología , Liofilización , Humanos , Músculo Liso/fisiología , Regeneración , Propiedades de SuperficieRESUMEN
Development and selection of an ideal scaffold is of importance for tissue engineering. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) is a biocompatible bioresorbable copolymer that belongs to the polyhydroxyalkanoate family. Because of its good biocompatibility, PHBHHx has been widely used as a cell scaffold for tissue engineering. This review focuses on the utilization of PHBHHx-based scaffolds in tissue engineering. Advances in the preparation, modification, and application of PHBHHx scaffolds are discussed.
Asunto(s)
Humanos , /química , Materiales Biocompatibles/química , Caproatos/química , Ingeniería de Tejidos/métodos , Andamios del Tejido/química , /uso terapéutico , Materiales Biocompatibles/uso terapéutico , Huesos/fisiología , Caproatos/uso terapéutico , Cartílago/fisiología , Liofilización , Músculo Liso/fisiología , Regeneración , Propiedades de SuperficieRESUMEN
BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5, α7, α10, ß1, ß2, ß4, γ and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits α2, ß1, ß4 and mAChR subunits M4 had abundant expression (2(-ΔCt) > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChRα7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor α7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells. CONCLUSIONS: ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.
Asunto(s)
Acetilcolina/metabolismo , Médula Ósea/fisiología , Cartílago/fisiología , Colinérgicos/metabolismo , Maxilar/metabolismo , Animales , Células de la Médula Ósea/metabolismo , Matriz Ósea/metabolismo , Calcificación Fisiológica/fisiología , Carnitina Aciltransferasas/genética , Carnitina Aciltransferasas/metabolismo , Regulación de la Expresión Génica/fisiología , Inmunohistoquímica , Masculino , Maxilar/citología , Células Madre Mesenquimatosas/metabolismo , Proteínas de Transporte de Catión Orgánico/genética , Proteínas de Transporte de Catión Orgánico/metabolismo , Osteogénesis/fisiología , Ratas Sprague-Dawley , Reacción en Cadena en Tiempo Real de la Polimerasa , Receptores Nicotínicos/genética , Proteínas de Transporte Vesicular de Acetilcolina/genética , Proteínas de Transporte Vesicular de Acetilcolina/metabolismoRESUMEN
BACKGROUND: Acetylcholine (ACh) is known to be a key neurotransmitter in the central and peripheral nervous systems, which is also produced in a variety of non-neuronal tissues and cell. The existence of ACh in maxilla in vivo and potential regulation role for osteogenesis need further study. RESULTS: Components of the cholinergic system (ACh, esterase, choline acetyltransferase, high-affinity choline uptake, n- and mAChRs) were determined in maxilla of rat in vivo, by means of Real-Time PCR and immunohistochemistry. Results showed RNA for CarAT, carnitine/acylcarnitine translocase member 20 (Slc25a20), VAChT, OCTN2, OCT1, OCT3, organic cation transporter member 4 (Slc22a4), AChE, BChE, nAChR subunits α1, α2, α3, α5, α7, α10, ß1, ß2, ß4, γ and mAChR subunits M1, M2, M3, M4, M5 were detected in rat's maxilla. RNA of VAChT, AChE, nAChR subunits α2, ß1, ß4 and mAChR subunits M4 had abundant expression (2(-ΔCt) > 0.03). Immunohistochemical staining was conducted for ACh, VAChT, nAChRα7 and AChE. ACh was expressed in mesenchymal cells, chondroblast, bone and cartilage matrix and bone marrow cells, The VAChT expression was very extensively while ACh receptor α7 was strongly expressed in newly formed bone matrix of endochondral and bone marrow ossification, AchE was found only in mesenchymal stem cells, cartilage and bone marrow cells. CONCLUSIONS: ACh might exert its effect on the endochondral and bone marrow ossification, and bone matrix mineralization in maxilla.
Asunto(s)
Animales , Masculino , Ratas , Médula Ósea/fisiología , Acetilcolina/metabolismo , Cartílago/fisiología , Colinérgicos/metabolismo , Maxilar/metabolismo , Osteogénesis/fisiología , Matriz Ósea/metabolismo , Calcificación Fisiológica/fisiología , Células de la Médula Ósea/metabolismo , Inmunohistoquímica , Carnitina Aciltransferasas/genética , Carnitina Aciltransferasas/metabolismo , Regulación de la Expresión Génica/fisiología , Receptores Nicotínicos/genética , Ratas Sprague-Dawley , Proteínas de Transporte de Catión Orgánico/genética , Proteínas de Transporte de Catión Orgánico/metabolismo , Proteínas de Transporte Vesicular de Acetilcolina/genética , Proteínas de Transporte Vesicular de Acetilcolina/metabolismo , Células Madre Mesenquimatosas/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Maxilar/citologíaRESUMEN
Tissue banks around the world store human cartilage obtained from cadaveric donors for use in diverse reconstructive surgical procedures. To ensure this tissue is sterile at the time of distribution, tissues may be sterilized by ionizing radiation. In this work, we evaluate the physical changes in deep frozen costal cartilage (-70 °C) or costal cartilage preserved in high concentrations of glycerol (>98 %) followed by a terminal sterilization process using ionizing radiation, at 3 different doses (15, 25 and 50 kGy). Tension and compression tests were carried out to determine the mechanical changes related both to the different preservation methods and irradiation doses. For both methods of preservation, tension strength was increased by about 24 %, when cartilage tissue was irradiated with 15 kGy. Deep frozen samples, when irradiated with 25 or 50 kGy, had a decrease in their mechanical performance, albeit to a lesser extent than when tissues were preserved in high concentration of glycerol and equally irradiated. In conclusion, processing in high concentration of glycerol did not increase tissue protection against radiation damage; while cartilage preserved in high concentrations of glycerol withstands radiation up to 25 kGy, deep frozen human costal cartilage may be sterilized with a doses up to 50 kGy without significant mechanical impact.
Asunto(s)
Cartílago/fisiología , Cartílago/efectos de la radiación , Radiación Ionizante , Costillas/fisiología , Costillas/efectos de la radiación , Conservación de Tejido , Adolescente , Adulto , Fenómenos Biomecánicos/efectos de la radiación , Femenino , Humanos , Masculino , Persona de Mediana Edad , Estrés Mecánico , Adulto JovenRESUMEN
Cartilage is a specialized tissue represented by a group of particular cells (the chondrocytes) and an abundant extracellular matrix. Because of the reduced regenerative capacity of this tissue, cartilage injuries are often difficult to handle. Nowadays tissue engineering has emerged as a very promising discipline, and biodegradable polymeric scaffolds are widely used as tissue supports. In cartilage injuries, the use of autologous chondrocyte implantation from non-affected cartilage zones has emerged as a very interesting technique, where chondrocytes are expanded in order to obtain a greater number of cells. Nevertheless, it has been reported that chondrocytes in bidimensional cultures suffer a dedifferentiation process. The present study sought, in the first place, to standardize a novel protocol in order to obtain primary cultures of chondrocytes from newborn rabbit hyaline cartilage from the xiphoid process. Second, the potential of porous three-dimensional (3D) biodegradable polymeric matrices as support materials for chondrocytes was evaluated: a novel poly(ε-caprolactone)-poly(p-dioxanone) (PCL-PPDX) blend in a 90:10 w:w ratio and poly(ε-caprolactone) (PCL). After achieving the standardization, a typical round-shaped chondrocyte morphology and the expression of collagen type II and aggrecan, evaluated by RT-PCR, were observed. Second-passage chondrocytes adhered effectively to these scaffolds, although cell growth at 7 days in culture was significantly less in the PCL-PPDX blend. After 3 weeks of culture on PCL-PPDX or PCL, the cells expressed collagen type II. The present study demonstrates the potential, unknown until now, of PCL-PPDX blend scaffolds in the field of cartilage tissue engineering.
Asunto(s)
Cartílago/efectos de los fármacos , Cartílago/fisiología , Dioxanos/farmacología , Ensayo de Materiales , Poliésteres/farmacología , Polímeros/farmacología , Ingeniería de Tejidos/métodos , Andamios del Tejido/química , Animales , Cartílago/citología , Adhesión Celular/efectos de los fármacos , Comunicación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Separación Celular , Condrocitos/citología , Condrocitos/efectos de los fármacos , Condrocitos/metabolismo , Condrocitos/ultraestructura , Regulación de la Expresión Génica/efectos de los fármacos , Microscopía Electrónica de Rastreo , Porosidad/efectos de los fármacos , ARN Mensajero/genética , ARN Mensajero/metabolismo , ConejosRESUMEN
In cartilaginous tissues, perichondrium cambium layer may be the source of new cartilage. Human nasal septal perichondrium is considered to be a homogeneous structure in which some authors do not recognize the perichondrium internal zone or the cambium layer as a layer distinct from adjacent cartilage surface. In the present study, we isolated a chondrogenic cell population from human nasal septal cartilage surface zone. Nasoseptal chondrogenic cells were positive for surface markers described for mesenchymal stem cells, with exception of CD146, a perivascular cell marker, which is consistent with their avascular niche in cartilage. Although only Sox-9 was constitutively expressed, they also revealed osteogenic and chondrogenic, but not adipogenic, potentials in vitro, suggesting a more restricted lineage potential compared to mesenchymal stem cells. Interestingly, even in absence of chondrogenic growth factors in the pellet culture system, nasoseptal chondrogenic cells had a capacity to synthesize sulfated glycosaminoglycans, large amounts of collagen type II and to a lesser extent collagen type I. The spontaneous chondrogenic potential of this population of cells indicates that they may be a possible source for cartilage tissue engineering. Besides, the pellet culture system using nasoseptal chondrogenic cells may also be a model for studies of chondrogenesis.
Asunto(s)
Cartílago/fisiología , Separación Celular/métodos , Condrocitos/citología , Condrogénesis , Tabique Nasal/citología , Ingeniería de Tejidos/métodos , Adipogénesis , Adulto , Técnicas de Cultivo de Célula , Linaje de la Célula , Condrocitos/ultraestructura , Humanos , Tabique Nasal/ultraestructura , OsteogénesisRESUMEN
A variety of patch materials has been used to close large atrial septal defects (ASD). Autologous pericardium and glutaraldehyde-preserved bovine pericardium are the most used. Lyophilized bovine pericardium has not been tested inside the cardiovascular system. The aim of this work was to study the behaviour and effectiveness of lyophilized glutaraldehyde-preserved bovine pericardium in ASD closure. Sixteen mongrel dogs were operated on. A 3 cm diameter atrial septal defect was created, and closed with: Group I (n=8): Lyophilized glutaraldehyde preserved bovine pericardium (LGPBP). Group II (n=8): Vascular Dacron patch. The animals were evaluated clinically, by echocardiography, macroscopically, and microscopically. Statistical analysis was done with analysis of variance (ANOVA) and Student's t-test. All the animals survived the surgical procedure and study time (6 months). Clinically all the animals displayed normal physical activity, with normal cardiac sounds. Echocardiography showed that both groups had a normal heart without intracardiac shunts, no thrombus formation, and no vegetations. Macroscopically all the animals showed good integration of the lyophilized bioprosthesis and Dacron patch. All group I animals presented a decrease of the area of the ASD in the left atrium (p<0.001 by ANOVA and Student's t-test). Microscopically, group I presented dense and well-organized collagenous tissue, areas of cartilaginous metaplasia and remnants of the lyophilized bioprosthesis (p<0.001 by ANOVA and Student's t-test). Group II showed encapsulated Dacron patch covered with collagenous tissue and cartilaginous metaplasia. In conclusion, the new lyophilized bioprosthesis is well integrated into the atrial septum, without complications and is effective for ASD closure.
Asunto(s)
Materiales Biocompatibles/farmacología , Defectos del Tabique Interatrial/cirugía , Ensayo de Materiales/métodos , Pericardio/trasplante , Prótesis e Implantes/normas , Implantación de Prótesis/métodos , Animales , Materiales Biocompatibles/uso terapéutico , Procedimientos Quirúrgicos Cardíacos/instrumentación , Procedimientos Quirúrgicos Cardíacos/métodos , Cartílago/citología , Cartílago/fisiología , Bovinos , Colágeno/metabolismo , Modelos Animales de Enfermedad , Perros , Ecocardiografía , Fijadores , Liofilización/métodos , Glutaral , Defectos del Tabique Interatrial/diagnóstico por imagen , Defectos del Tabique Interatrial/patología , Tabiques Cardíacos/diagnóstico por imagen , Tabiques Cardíacos/patología , Tabiques Cardíacos/cirugía , Pericardio/química , Pericardio/efectos de los fármacos , Tereftalatos Polietilenos/uso terapéutico , Complicaciones Posoperatorias , Prótesis e Implantes/tendencias , Fijación del Tejido/métodos , Resultado del TratamientoRESUMEN
Cartilage tissue has a poor capacity for self-repair, especially in the case of severe cartilage damage due to trauma or age-related degeneration. Cell-based tissue engineering using scaffolds has provided an option for the repair of cartilage tissue. The present work demonstrates that a three-dimensional (3D) chitosan scaffold increases the efficiency of the adhesion and differentiation of mesenchymal stem cells (MSCs) after the addition of a chondrogenic medium. These culture conditions promoted MSC differentiation into chondrocytes during the first 9 weeks of monolayer or 3D culture in a scaffold composed of chitosan or chitosan/gelatin. The results demonstrated that a chitosan scaffold caused a reduction in alkaline phosphatase production and an increase in the collagen concentration indicating phenotypic changes in the cells. In support of these results, the production of collagen type II by the MSCs cultured in the chitosan scaffold increased after 3 weeks of culture, indicating the beginning of differentiation. However, the addition of gelatin to the chitosan scaffold did not improve on the results obtained with chitosan alone. These results suggest that this 3D chitosan scaffold is a promising candidate for biomaterial implants designed to promote MSC colonization and has applications in regenerative medicine.
Asunto(s)
Diferenciación Celular/efectos de los fármacos , Quitosano/farmacología , Condrocitos/citología , Células Madre Mesenquimatosas/citología , Ingeniería de Tejidos/métodos , Fosfatasa Alcalina/metabolismo , Animales , Cartílago/fisiología , Células Cultivadas , Condrocitos/metabolismo , Colágeno/biosíntesis , Gelatina/farmacología , RatasRESUMEN
Functional orthopedic appliances correct dental malocclusion partially by exerting indirect mechanical stimulus on the condylar cartilage, modulating growth and the adaptation of orofacial structures. However, the exact nature of the biological responses to this therapy is not well understood. Insulin-like growth factors I and II (IGF-I and II) are important local factors during growth and differentiation in the condylar cartilage [D. Hajjar, M.F. Santos and E.T. Kimura, Propulsive appliance stimulates the synthesis of insulin-like growth factors I and II in the mandibular condylar cartilage of young rats, Arch. Oral Biol. 48 (2003), 635-642]. The bioefficacy of IGFs at the cellular level is modulated by IGF binding proteins (IGFBP). The aim of this study was to verify the mRNA and protein expression of IGFBP-3, IGFBP-4, IGFBP-5 and IGFBP-6 in the condylar cartilage of young male Wistar rats that used a mandibular propulsive appliance for 3, 9, 15, 20, 30 or 35 days. For this purpose, sagittal sections of decalcified and paraffin-embedded condyles were submitted to immunohistochemistry and the condylar cartilage to RT-PCR. The control group showed a gradual increase in the protein expression of all IGFBPs, except IGFBP-4. Following use of the appliance, IGFBP-3 and IGFBP-6 expression decreased in the early stage of the treatment. At 20 days of treatment there was a decline in the IGFs and IGFBP-3, IGFBP-4 and IGFBP-5 expression and at 30 days there was a peak in the IGFs and all IGFBPs expression except for IGFBP-3 where the peak was observed in the control animals. The expression patterns of all IGFBPs in the condylar cartilage were similar. The modulation of IGFBP-3, -4, -5 and -6 expression in the condylar cartilage in response to the propulsive appliance suggests that those peptides are involved in the mandibular adaptation during this therapy.
Asunto(s)
Cartílago/metabolismo , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Cóndilo Mandibular/metabolismo , Aparatos Ortodóncicos Funcionales , Animales , Cartílago/citología , Cartílago/fisiología , Condrocitos/fisiología , Regulación de la Expresión Génica , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/genética , Masculino , Cóndilo Mandibular/citología , Cóndilo Mandibular/fisiología , Mecanotransducción Celular/fisiología , ARN Mensajero/genética , Ratas , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Estrés MecánicoRESUMEN
Thirteen fresh equine heads were dissected in an attempt to determine the cause of a groove frequently found on the dorsal border and medial side of the nasal process of the incisive bone. This groove appeared on both sides in 40 out of 44 equine skulls. The sulcus seems to be caused by the combined action of the lateralis nasi muscle and the medial accessory cartilage of the nose (cartilago nasalis accessoria medialis). Other sulci found on the nasal process of the bone may be explained as impressions caused by nerves.
Asunto(s)
Caballos/anatomía & histología , Maxilar/anatomía & histología , Animales , Cartílago/anatomía & histología , Cartílago/fisiología , Caballos/fisiología , Músculos Masticadores/anatomía & histología , Músculos Masticadores/fisiología , Maxilar/inervaciónRESUMEN
Se realizó un estudio clínico-radiográfico, transversal, comparativo, en 300 individuos sanos comprendidos entre de 10 y 20 años, de los dos sexos, del Instituto Fiscal de Educación Integrado "José Martí" y del Colegio Nacional Santiago de Guayaquil de la ciudad de Quito, entre enero y diciembre de 1996, con el propósito de elaborar una tabla de valoración ósea nacional, usando como referencia el signo de RISSER, (evolución de los centros secundarios de osificación de la cresta ilíaca). A cada uno de los sujetos de nuestro estudio se le realizó una radiografía anteroposterior de pelvis, y se analizaron las siguientes variables: peso, talla, edad, sexo y perímetro braquia para valorar el estado nutricional. Los sujetos fueron subdivididos en dos grupos de acuerdo al sexo: hombres y mujeres, y de acuerdo a la edad en subgrupos de 10 a 20 años con intervalos de 1 año. Se encontró que la maduración ósea según el signo de Risser se inicia más temprano en las mujeres. En los hombres existe una relación directa (correlación positiva r = 0.50) entre el aparecimiento del signo de Risser con las siguientes variables: perímetro braquial a los 16 años y con el peso y la talla a los 13 años; en las mujeres con talla a los 10 años y peso y perímetro braquial a los 13 años. En los hombres el signo de RISSER se inicia a los 13 años en un 6.67 por ciento y a los 19 años únicamente han completado la osificación el 46.67 por ciento, en las mujeres el 6.67 por ciento presentaron un RISSER 1 a los 10 años y completaron su osificación el 54.55 por ciento a los 19 años. Ninguno de los grupos presentó signos de desnutrición (Indice de Quetelet 0.17-0.22). Estos valores comparados con los de la literatura, nos indican que el desarrollo óseo en hombres y mujeres de Quito es notoriamente más tardía debido probablemente a factores nutricionales, climáticos, genéticos y étnicos...
Asunto(s)
Humanos , Desarrollo Óseo , Cartílago/anatomía & histología , Cartílago/fisiología , Cartílago/patologíaAsunto(s)
Cartílago/citología , Cartílago/fisiología , Diferenciación Celular/efectos de los fármacos , Proteoglicanos/biosíntesis , Factor de Crecimiento Transformador beta/farmacología , Animales , Cartílago/efectos de los fármacos , Células Cultivadas , Cinética , Esbozos de los Miembros , Mesodermo/citología , Mesodermo/efectos de los fármacos , RatonesRESUMEN
The absolute concentrations of chondroitin 4- and 6-sulfate are compared in articular and endochondral ossification cartilage from normal dogs. In newborn dogs, the absolute concentration of chondroitin 4-sulfate decreases nearly 3-fold from the deeper endochondral ossification cartilage to the articular surface, whereas that of chondroitin 6-sulfate does not change. In cartilage from the articular surface of the epiphysis in adults, where the ossification process is complete, the concentration of chondroitin 4-sulfate is also low. These observations suggest that chondroitin 4-sulfate may be important in the ossification process. The pathogenesis of heritable disorders involving the sulfation of chondroitin sulfate is discussed in view of these findings.