RESUMEN
Glycosaminoglycans (GAGs) are essential functional components of the extracellular matrix (ECM). Artificial GAGs like sulfated hyaluronan (sHA) exhibit pro-osteogenic properties and boost healing processes. Hence, they are of high interest for supporting bone regeneration and wound healing. Although sulfated GAGs (sGAGs) appear intracellularly, the knowledge about intracellular effects and putative interaction partners is scarce. Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). Overall, 477 proteins were found interacting with at least one of four distinct sGAGs. Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. The interaction of sGAG with α2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and serine protease HTRA1 (HTRA1) was confirmed in reverse assays. Consecutive pathway and cluster analysis led to the identification of biological processes, namely processes involving binding and processing of nucleic acids, LRP1-dependent endocytosis, and exosome formation. Respecting the preferentially intracellular localization of sGAG in vesicle-like structures, also the interaction data indicate sGAG-specific modulation of vesicle-based transport processes. By identifying many sGAG-specific interacting proteins, our data provide a resource for upcoming studies aimed at molecular mechanisms and understanding of sGAG cellular effects.
Asunto(s)
Glicosaminoglicanos/metabolismo , Serina Peptidasa A1 que Requiere Temperaturas Altas/metabolismo , Carioferinas/metabolismo , Proteína Asociada a Proteínas Relacionadas con Receptor de LDL/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Células Cultivadas , Cromatografía Liquida , Glicosaminoglicanos/química , Serina Peptidasa A1 que Requiere Temperaturas Altas/química , Serina Peptidasa A1 que Requiere Temperaturas Altas/aislamiento & purificación , Humanos , Carioferinas/química , Carioferinas/aislamiento & purificación , Proteína Asociada a Proteínas Relacionadas con Receptor de LDL/química , Proteína Asociada a Proteínas Relacionadas con Receptor de LDL/aislamiento & purificación , Células Madre Mesenquimatosas/química , Células Madre Mesenquimatosas/metabolismo , Receptores Citoplasmáticos y Nucleares/química , Receptores Citoplasmáticos y Nucleares/aislamiento & purificación , Espectrometría de Masas en Tándem , Proteína Exportina 1RESUMEN
Nucleocytoplasmic transport of proteins with molar masses of larger than 60,000 is mediated by transport receptors. The transport receptor transportin1 (Trn1) transports various kinds of RNA-binding proteins such as JKTBP, hnRNP D and TAP. Trn1 was successfully cocrystallized with nucleocytoplasmic shuttling fragments of JKTBP and hnRNP D and a nuclear localization fragment of TAP. The crystal of the Trn1-JKTBP fragment complex belongs to space group P2(1)2(1)2, with unit-cell parameters a = 131.5, b =171.5, c = 68.2 angstroms. The crystals of Trn1 in complex with hnRNP D and TAP fragments are orthorhombic, space group P2(1)2(1)2(1), with unit-cell parameters a = 69.1, b = 119.1, c = 151.1 angstroms and a = 69.0, b = 119.1, c = 146.0 angstroms, respectively. The crystals diffracted to beyond 3.0, 3.2 and 2.4 angstroms resolution, respectively, using synchrotron radiation at SPring-8.
Asunto(s)
Carioferinas/química , Fragmentos de Péptidos/química , Cristalización , Cristalografía por Rayos X , Carioferinas/aislamiento & purificación , Carioferinas/metabolismo , Fragmentos de Péptidos/aislamiento & purificación , Conformación Proteica , Proteínas de Unión al ARN/química , SincrotronesRESUMEN
Expression of recombinant proteins as fusions to the eukaryotic protein ubiquitin has been found to significantly increase the yield of unstable or poorly expressed proteins. The benefit of this technique is further enhanced by the availability of naturally occurring deubiquitylating enzymes, which remove ubiquitin from the fusion product. However, the versatility of the system has been constrained due to the lack of a robust, easily purified deubiquitylating enzyme. Here we report the development of an efficient expression system, utilizing the ubiquitin fusion technique, which allows convenient high yield and easy purification of authentic protein. An Escherichia coli vector (pHUE) was constructed for the expression of proteins as histidine-tagged ubiquitin fusions, and a histidine-tagged deubiquitylating enzyme to cleave these fusions was expressed and purified. The expression system was tested using several proteins varying in size and complexity. These results indicate that this procedure will be suitable for the expression and rapid purification of a broad range of proteins and peptides, and should be amenable to high-throughput applications.