RESUMEN
Anal squamous cell carcinoma (ASCC) is a rare gastrointestinal malignancy linked to high-risk human papillomavirus (HPV) infection, which develops from precursor lesions like low-grade squamous intraepithelial lesions and high-grade squamous intraepithelial lesions (HGSILs). ASCC incidence varies across populations and poses increased risk for people living with HIV. Our investigation focused on transcriptomic and metatranscriptomic changes from squamous intraepithelial lesions to ASCC. Metatranscriptomic analysis highlighted specific bacterial species (e.g., Fusobacterium nucleatum, Bacteroides fragilis) more prevalent in ASCC than precancerous lesions. These species correlated with gene-encoding enzymes (Acca, glyQ, eno, pgk, por) and oncoproteins (FadA, dnaK), presenting potential diagnostic or treatment markers. Unsupervised transcriptomic analysis identified distinct sample clusters reflecting histological diagnosis, immune infiltrate, HIV/HPV status, and pathway activities, recapitulating anal cancer progression's natural history. Our study unveiled molecular mechanisms in anal cancer progression, aiding in stratifying HGSIL cases based on low or high risk of progression to malignancy.
Asunto(s)
Neoplasias del Ano , Carcinoma de Células Escamosas , Transcriptoma , Humanos , Neoplasias del Ano/genética , Neoplasias del Ano/inmunología , Neoplasias del Ano/patología , Neoplasias del Ano/virología , Neoplasias del Ano/microbiología , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/inmunología , Carcinoma de Células Escamosas/microbiología , Carcinoma de Células Escamosas/patología , Microbiota/inmunología , Masculino , Infecciones por Papillomavirus/complicaciones , Infecciones por Papillomavirus/genética , Infecciones por Papillomavirus/virología , Infecciones por Papillomavirus/inmunología , Lesiones Intraepiteliales Escamosas/genética , Lesiones Intraepiteliales Escamosas/patología , Lesiones Intraepiteliales Escamosas/virología , Femenino , Progresión de la Enfermedad , Persona de Mediana Edad , Infecciones por VIH/complicaciones , Infecciones por VIH/inmunologíaRESUMEN
There is increasing evidence showing positive association between changes in oral microbiome and the occurrence of oral squamous cell carcinoma (OSCC). Alcohol- and nicotine-related products can induce microbial changes but are still unknown if these changes are related to cancerous lesion sites. In an attempt to understand how these changes can influence the OSCC development and maintenance, the aim of this study was to investigate the oral microbiome linked with OSCC as well as to identify functional signatures and associate them with healthy or precancerous and cancerous sites. Our group used data of oral microbiomes available in public repositories. The analysis included data of oral microbiomes from electronic cigarette users, alcohol consumers, and precancerous and OSCC samples. An R-based pipeline was used for taxonomic and functional prediction analysis. The Streptococcus spp. genus was the main class identified in the healthy group. Haemophilus spp. predominated in precancerous lesions. OSCC samples revealed a higher relative abundance compared with the other groups, represented by an increased proportion of Fusobacterium spp., Prevotella spp., Haemophilus spp., and Campylobacter spp. Venn diagram analysis showed 52 genera exclusive of OSCC samples. Both precancerous and OSCC samples seemed to present a specific associated functional pattern. They were menaquinone-dependent protoporphyrinogen oxidase pattern enhanced in the former and both 3',5'-cyclic-nucleotide phosphodiesterase (purine metabolism) and iron(III) transport system ATP-binding protein enhanced in the latter. We conclude that although precancerous and OSCC samples present some differences on microbial profile, both microbiomes act as "iron chelators-like" potentially contributing to tumor growth.
Asunto(s)
Carcinoma de Células Escamosas , Hierro/metabolismo , Microbiota , Neoplasias de la Boca , Microambiente Tumoral , Consumo de Bebidas Alcohólicas , Carcinoma de Células Escamosas/microbiología , Sistemas Electrónicos de Liberación de Nicotina , Compuestos Férricos/metabolismo , Humanos , Neoplasias de la Boca/microbiología , Lesiones Precancerosas/microbiologíaRESUMEN
OBJECTIVE:: Esophageal squamous cell carcinoma is one of the most common esophageal diseases in the developing world, but the relationship between esophageal squamous cell carcinoma and Helicobacter pylori infection remains a neglected topic. The primary objective of this study was to determine the association between Helicobacter pylori infection and esophageal squamous cell carcinoma. A second purpose was to determine the incidence and factors associated with Helicobacter pylori infection following esophagectomy. METHOD:: The microorganism was identified by testing the gastric biopsy materials from 95 esophageal squamous cell carcinoma patients (66 females; 39 were esophagectomized) for urease activity in a medium containing urea and a power of hydrogen detection reagent and comparing the results with those from a healthy population. Differences in patient characteristics were assessed with chi-square tests and t-tests for categorical and continuous factors, respectively. RESULTS:: The patients with esophageal squamous cell carcinoma had a significantly lower prevalence of Helicobacter pylori compared with the healthy population (p<0.001). The naive and esophagectomized patients, in contrast, showed no significant differences in Helicobacter pylori infection (p>0.005). Patients with esophageal squamous cell carcinoma showed a significant association between leukocytosis and hypoglobulinemia and the presence of Helicobacter pylori infection (p=0.023 and p=0.045, respectively). CONCLUSION:: These results suggest that Helicobacter pylori is not an etiological factor in patients with esophageal squamous cell carcinoma. We found a statistically significant negative correlation between esophageal squamous cell cancer and Helicobacter pylori infection. These findings may guide new strategies for esophageal squamous cell carcinoma therapy.
Asunto(s)
Carcinoma de Células Escamosas/microbiología , Neoplasias Esofágicas/microbiología , Infecciones por Helicobacter/complicaciones , Helicobacter pylori/aislamiento & purificación , Adulto , Anciano , Anciano de 80 o más Años , Biopsia , Carcinoma de Células Escamosas/patología , Estudios de Casos y Controles , Neoplasias Esofágicas/patología , Carcinoma de Células Escamosas de Esófago , Femenino , Infecciones por Helicobacter/patología , Helicobacter pylori/patogenicidad , Humanos , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Factores de Riesgo , Factores Sexuales , Estadísticas no ParamétricasRESUMEN
OBJECTIVE: Esophageal squamous cell carcinoma is one of the most common esophageal diseases in the developing world, but the relationship between esophageal squamous cell carcinoma and Helicobacter pylori infection remains a neglected topic. The primary objective of this study was to determine the association between Helicobacter pylori infection and esophageal squamous cell carcinoma. A second purpose was to determine the incidence and factors associated with Helicobacter pylori infection following esophagectomy. METHOD: The microorganism was identified by testing the gastric biopsy materials from 95 esophageal squamous cell carcinoma patients (66 females; 39 were esophagectomized) for urease activity in a medium containing urea and a power of hydrogen detection reagent and comparing the results with those from a healthy population. Differences in patient characteristics were assessed with chi-square tests and t-tests for categorical and continuous factors, respectively. RESULTS: The patients with esophageal squamous cell carcinoma had a significantly lower prevalence of Helicobacter pylori compared with the healthy population (p<0.001). The naive and esophagectomized patients, in contrast, showed no significant differences in Helicobacter pylori infection (p>0.005). Patients with esophageal squamous cell carcinoma showed a significant association between leukocytosis and hypoglobulinemia and the presence of Helicobacter pylori infection (p=0.023 and p=0.045, respectively). CONCLUSION: These results suggest that Helicobacter pylori is not an etiological factor in patients with esophageal squamous cell carcinoma. We found a statistically significant negative correlation between esophageal squamous cell cancer and Helicobacter pylori infection. These findings may guide new strategies for esophageal squamous cell carcinoma therapy.
Asunto(s)
Humanos , Masculino , Femenino , Adulto , Persona de Mediana Edad , Anciano , Anciano de 80 o más Años , Carcinoma de Células Escamosas/microbiología , Neoplasias Esofágicas/microbiología , Infecciones por Helicobacter/complicaciones , Helicobacter pylori/aislamiento & purificación , Biopsia , Carcinoma de Células Escamosas/patología , Estudios de Casos y Controles , Neoplasias Esofágicas/patología , Infecciones por Helicobacter/patología , Helicobacter pylori/patogenicidad , Estudios Retrospectivos , Factores de Riesgo , Factores Sexuales , Estadísticas no ParamétricasRESUMEN
Fanconi anemia (FA) is a rare genetic disease characterized by chromosomal instability and impaired DNA damage repair. FA patients develop oral squamous cell carcinoma (OSCC) earlier and more frequently than the general population, especially after hematopoietic stem cell transplantation (HSCT). Although evidence of an etiological role of the local microbiome and carcinogenesis has been mounting, no information exists regarding the oral microbiome of FA patients. The aim of this study was to explore the salivary microbiome of 61 FA patients regarding their oral health status and OSCC risk factors. After answering a questionnaire and receiving clinical examination, saliva samples were collected and analyzed using 16S rRNA sequencing of the V3-V4 hypervariable region. The microbial profiles associated with medical and clinical parameters were analyzed using general linear models. Patients were young (mean age, 22 y) and most had received HSCT ( n = 53). The most abundant phyla were Firmicutes [mean relative abundance (SD), 42.1% (10.1%)] and Bacteroidetes [(25.4% (11.4%)]. A history of graft-versus-host disease (GVHD) ( n = 27) was associated with higher proportions of Firmicutes (43.8% × 38.5%, P = 0.05). High levels of gingival bleeding were associated with the genera Prevotella (22.25% × 20%), Streptococcus (19.83% × 17.61%), Porphyromonas (3.63% × 1.42%, P = 0.03), Treponema (1.02% × 0.28%, P = 0.009), Parvimonas (0.28% × 0.07%, P = 0.02) and Dialister (0.27% × 0.10%, P = 0.04). Finally, participants transplanted over 11 y ago showed the highest levels of Streptococcus (18.4%), Haemophilus (12.7%) and Neisseria (6.8%). In conclusion, FA patients that showed poor oral hygiene harbored higher proportions of the genera of bacteria compatible with gingival disease. Specific microbial differences were associated with a history of oral GVHD and a history of oral mucositis.
Asunto(s)
Carcinoma de Células Escamosas/microbiología , Anemia de Fanconi/complicaciones , Microbiota , Neoplasias de la Boca/microbiología , Saliva/microbiología , Factores de Edad , Anemia de Fanconi/terapia , Femenino , Hemorragia Gingival/microbiología , Enfermedad Injerto contra Huésped/microbiología , Trasplante de Células Madre Hematopoyéticas , Humanos , Masculino , Mucositis/microbiología , Higiene Bucal , Factores de Riesgo , Adulto JovenAsunto(s)
Carcinoma de Células Escamosas/diagnóstico por imagen , Neoplasias Pulmonares/diagnóstico por imagen , Aspergilosis Pulmonar/diagnóstico por imagen , Toracoscopía/métodos , Anciano , Antifúngicos/administración & dosificación , Broncoscopios , Carcinoma de Células Escamosas/microbiología , Humanos , Neoplasias Pulmonares/microbiología , Masculino , Aspergilosis Pulmonar/tratamiento farmacológico , Toracoscopía/instrumentación , Resultado del Tratamiento , Voriconazol/administración & dosificaciónRESUMEN
INTRODUCTION: Paracoccidioidomycosis is a disease that is endemic to southern and southeastern Brazil, caused by the fungus Paracoccidioides brasiliensis. The most common clinical oral manifestation is the presence of multiple granulomatous ulcers with hemorrhagic dots, located mainly on the lips, palate, and buccal mucosa. However, the disease can manifest atypically as a single ulcer, mimicking oral squamous cell carcinoma (SCC) or tuberculosis. CASE REPORT: A 65-year-old male patient presented with a complaint of a single ulcerated lesion on the dorsum of the tongue; the lesion had evolved over 6 months. The diagnostic hypotheses were SCC and oral manifestation of tuberculosis. An incisional biopsy was performed, and histopathological analysis of the specimen revealed pseudoepitheliomatous hyperplasia, a granulomatous structure of epithelioid histiocytes, multinucleated giant cells, and lymphocytes in the connective tissue. Grocott staining confirmed the presence of the fungus in the lesion, and a diagnosis was made of paracoccidioidomycosis. The patient was treated with 200 mg/day of itraconazole for 12 months and now shows no signs or symptoms of recurrence of the disease. CONCLUSION: Correct diagnosis is essential for a successful therapeutic approach and resolution of the lesion.
Asunto(s)
Carcinoma de Células Escamosas/patología , Paracoccidioides/fisiología , Paracoccidioidomicosis/patología , Enfermedades de la Lengua/patología , Anciano , Antifúngicos/uso terapéutico , Brasil , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/tratamiento farmacológico , Carcinoma de Células Escamosas/microbiología , Humanos , Itraconazol/uso terapéutico , Masculino , Paracoccidioides/aislamiento & purificación , Paracoccidioidomicosis/diagnóstico , Paracoccidioidomicosis/tratamiento farmacológico , Paracoccidioidomicosis/microbiología , Enfermedades de la Lengua/diagnóstico , Enfermedades de la Lengua/tratamiento farmacológico , Enfermedades de la Lengua/microbiologíaRESUMEN
INTRODUCTION: Several studies have implicated Helicobacter pylori as a risk factor in laryngeal cancer, but other studies disagree. It is fundamental that the relationship between Helicobacter pylori and laryngeal cancer be verified in order to provide evidence of ways to prevent the initiation and development of this carcinoma. MATERIALS AND METHODS: In total, 81 patients with laryngeal squamous cell carcinoma and 75 control subjects were enrolled in a case-control study. Semi-nested polymerase chain reaction techniques were applied to detect Helicobacter pylori in the laryngeal mucosa and enzyme-linked immunosorbent assays were used to detect serum antibodies against Helicobacter pylori. Risk factors associated with laryngeal carcinoma were analyzed using logistic regression models. RESULTS: The presence of Helicobacter pylori in the larynx was higher in patients with laryngeal cancer than in control subjects (71.6 vs. 25.3 %, p < 0.001). Among patients with laryngeal carcinoma, rates of Helicobacter pylori infection were higher in normal laryngeal tissues than in tumor tissues. After adjusting for confounding factors, regression analysis indicated that the microbe was an independent risk factor for laryngeal cancer (OR = 7.15, 95 % CI [3.29, 15.53], p < 0.001). CONCLUSIONS: This study suggests that Helicobacter pylori is present in the mucosa of the larynx. The microorganism may be an independent risk factor for laryngeal squamous cell carcinoma. The laryngeal mucosa thus provides a reservoir for the bacteria possibly, and is a likely staging place for its transmission to other areas.
Asunto(s)
Carcinoma de Células Escamosas/etiología , Infecciones por Helicobacter/complicaciones , Helicobacter pylori , Neoplasias Laríngeas/etiología , Carcinoma de Células Escamosas/microbiología , Carcinoma de Células Escamosas/patología , Estudios de Casos y Controles , Infecciones por Helicobacter/patología , Humanos , Neoplasias Laríngeas/microbiología , Neoplasias Laríngeas/patología , Modelos Logísticos , Factores de RiesgoRESUMEN
Candidosis is the most common fungal infection in the oral cavity, and is usually associated with local and systemic predisposing factors. The occurrence and relevance of Candidal infection in oral lesions such as liquen planus, leukoplakias and carcinomas are still to be understood. The aim of the present study was to define the frequency of infection by Candida sp. on biopsies of oral mucosal lesions and associate its presence with malignant and dysplastic lesions. Histopathology reports issued between 1990 and 2001 inclusive were reviewed. Three sections of each mucosal biopsy were stained using the periodic acid-Schiff (PAS) technique. From the 832 biopsies 27.2% were PAS positive, of which 83.25% were obtained from male patients. There was positive association between fungic infection and mild, moderate and severe epithelial dysplasia, squamous cell carcinoma and hiperqueratosis (p < 0.05). There was no association between fungic infection and inflammatory fibrous hyperplasia, hyperkeratosis, lichen planus and pyogenic granuloma (p < 0.05). The frequency of infection in the tongue was significantly higher (p < 0.05) than in the other sites. Our results do not show a causal relation between Candida sp. and dysplastic lesions and carcinomas, but do confirm the higher presence of that microrganism in those lesions.
Asunto(s)
Candidiasis Bucal/microbiología , Carcinoma de Células Escamosas/microbiología , Mucosa Bucal/microbiología , Neoplasias de la Boca/microbiología , Lesiones Precancerosas/microbiología , Biopsia , Candida/aislamiento & purificación , Candidiasis Bucal/patología , Recuento de Colonia Microbiana , Femenino , Humanos , Masculino , Mucosa Bucal/patologíaRESUMEN
The aim of this study was to quantify the microorganisms Streptococcus mutans and Candida sp in the oral cavity of patients with oropharynx carcinoma, before, during and after radiotherapy, and to correlate the results with salivary factors such as pH, buffer capacity (CT) and flow rate (FS). Saliva samples were collected, diluted and inoculated in SB-20 agar and in Sabouraud agar, for Streptococcus mutans and Candida sp, respectively. Previously to dilution, the concentrated saliva was analyzed, and the salivary factors were determined. After the growth of colonies, the number of microorganisms was determined in CFU/ml. The analysis of the results allowed to conclude that the salivary factors are related to the presence of microorganisms, and that the number of CFU/ml increased as salivary flow rate decreased. The effects of radiation compromised salivary homeostasis and favored the increase of infection by yeasts and bacteria.
Asunto(s)
Candida/aislamiento & purificación , Carcinoma de Células Escamosas/microbiología , Neoplasias Orofaríngeas/microbiología , Saliva/microbiología , Streptococcus mutans/aislamiento & purificación , Análisis de Varianza , Carcinoma de Células Escamosas/radioterapia , Humanos , Concentración de Iones de Hidrógeno , Neoplasias Orofaríngeas/radioterapia , Saliva/química , Saliva/metabolismoRESUMEN
The relationship between the histological grading of malignancy and p53 protein expression was studied in 40 biopsies of oral squamous cell carcinomas. An immunohistochemical analysis was carried out using the steptavidin method preceded by a treatment with citric acid in a microwave oven. All cases were classified according to the histological malignancy grading system proposed by Anneroth et al. (Scandinavian Journal of Dental Research 1987, 95, 229-249). The expression of p53 was found in 62.5 percent of the carcinomas studied. Positivity of p53 staining showed a correlation with the histological grade of malignancy and with the degree of keratinisation, nuclear polymorphism and number of mitoses
Asunto(s)
Biopsia , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/microbiología , Neoplasias de la Boca , InmunohistoquímicaRESUMEN
To assess the association between human papillomavirus (HPV) and cervical cancer we have carried out two case-control studies of cervical intraepithelial neoplasia grade III (CIN III) (525 cases and 512 matched controls) and two case-control studies of invasive squamous cell carcinoma (436 cases and 387 population controls) in Cali, Colombia and nine provinces of Spain. HPV DNA detected by polymerase chain reaction, a PCR-based hybridization assay in the exfoliated cells of the uterine cervix, was the strongest risk factor in both countries. For invasive cancer the adjusted odds ratios and 95% confidence intervals were: 46.2 (18.5-115.1) in Spain and 15.6 (6.9-34.7) in Columbia and for CIN III they were: 56.9 (24.8-130.6) in Spain and 15.5 (8.2-29.4) in Columbia. This strong association was specific for types 16, 18, 31, 33 and 35 and also for HPV types not yet characterized. Hormonal factors such as oral contraceptives and high parity appear to confer an additional risk increasing the progression from chronic HPV infection to cancer. Our overall results indicate that HPV is the main cause of cervical cancer in two countries with contrasting rates of cervical cancer, Columbia having an incidence rate about 8 times higher than Spain.
Asunto(s)
Carcinoma de Células Escamosas/epidemiología , Papillomaviridae/aislamiento & purificación , Displasia del Cuello del Útero/epidemiología , Neoplasias del Cuello Uterino/epidemiología , Neoplasias del Cuello Uterino/microbiología , Adolescente , Adulto , Carcinoma de Células Escamosas/etiología , Carcinoma de Células Escamosas/microbiología , Carcinoma de Células Escamosas/patología , Estudios de Casos y Controles , Cuello del Útero/microbiología , Cuello del Útero/patología , Colombia/epidemiología , Anticonceptivos Orales , ADN Viral/análisis , Femenino , Humanos , Invasividad Neoplásica , Papillomaviridae/genética , Paridad , Reacción en Cadena de la Polimerasa/métodos , Factores de Riesgo , Conducta Sexual , Fumar , España/epidemiología , Displasia del Cuello del Útero/microbiología , Displasia del Cuello del Útero/patología , Displasia del Cuello del Útero/etiología , Neoplasias del Cuello Uterino/etiología , Neoplasias del Cuello Uterino/patologíaRESUMEN
Paraffinized tissue from Barbadian women with histologically proven genital carcinoma was subjected to a consensus polymerase chain reaction method. Nineteen patients had cervical and one, vaginal carcinoma. The histological types were 17 squamous cell carcinoma, 2 adenocarcinoma and 1 adenosquamous carcinoma. HPV DNA was detected in 18/20 (90%). HPV DNA type 16 in 13 (65%), type 33 and type 45 in 1 (5%) each and 3 (15%) could not be typed. HPV DNA, type 16, was detected in one (50%) of the two cases of adenocarcinoma and 12/17 (71%) cases of squamous cell carcinoma. DNA HPV, type 33, and type 45 were each detected in 1/17 (6%) cases of squamous cell carcinoma. No HPV DNA, type 18, was detected.
Asunto(s)
Reacción en Cadena de la Polimerasa , Neoplasias del Cuello Uterino/genética , Neoplasias Vaginales/genética , Adenocarcinoma/genética , Adenocarcinoma/microbiología , Adenocarcinoma/patología , Adulto , Anciano , Anciano de 80 o más Años , Secuencia de Bases , Carcinoma Adenoescamoso/genética , Carcinoma Adenoescamoso/microbiología , Carcinoma Adenoescamoso/patología , Carcinoma de Células Escamosas/genética , Carcinoma de Células Escamosas/microbiología , Carcinoma de Células Escamosas/patología , Sondas de ADN de HPV/análisis , ADN Viral/análisis , Femenino , Humanos , Persona de Mediana Edad , Datos de Secuencia Molecular , Papillomaviridae/genética , Infecciones por Papillomavirus/genética , Infecciones por Papillomavirus/microbiología , Infecciones por Papillomavirus/patología , Neoplasias del Cuello Uterino/microbiología , Neoplasias del Cuello Uterino/patología , Neoplasias Vaginales/microbiología , Neoplasias Vaginales/patologíaRESUMEN
Paraffinized tissue from Barbadian women with histologically proven gential carcinoma was subjected to a consensus polymerase chain reaction method. Nineteen patients had cervical and one, vaginal carcinoma. The histological types were 17 squamous cell carcinoma, 2 adenocarcinoma and 1 adenosquamous carcinoma. HPVDNA was detected in 18/20 (90 percent). HPVDNA type 16 in 13 (65 percent), type 33 and type 45 in 1 (5 percent) each and 3 (15 percent) could not be typed. HPVDNA, type 16, was detected in one (50 percent) of the two cases of adenocarcinoma and 12/17 (71 percent) cases of squamous cell carcinoma. DNAHPV, type 33, and type 45 were each detected in 1/17 (6 percent) cases of squamous cell carcinoma. No HPVDNA, type 18, was detected (AU)
Asunto(s)
Humanos , Adulto , Persona de Mediana Edad , Anciano , Femenino , /genética , ADN Viral , Neoplasias del Cuello Uterino/microbiología , Neoplasias Vaginales/microbiología , Reacción en Cadena de la Polimerasa , Virus Oncogénicos , Carcinoma de Células Escamosas/microbiología , Adenocarcinoma/microbiología , Sondas de ADN de HPV , BarbadosRESUMEN
Paraffinized tissue from Barbadian women with histologically proven gential carcinoma was subjected to a censensus polymerase chain reaction method. Nineteen patients had cervical and one, vaginal carcinoma. The histological types were 17 squamous cell carcinoma, 2 adenocarcinoma and 1 adenosquamous carcinoma. HPVDNA was detected in 18/20 (90 per cent ). HPVDNA type 16 in 13 (65 per cent ), type 33 and type 45 in 1 (5 per cent ) each and 3 (15 per cent ) could not be typed. HPVDNA, type 16, was detected in one (50 per cent ) of the two cases of adenocarcinoma and 12/17 (71 per cent ) cases of squamous cell carcinoma. DNAHPV, type 33, and type 45 were each detected in 1/17 (6 per cent ) cases of squamous cell carcinoma. No HPVDNA, type 18, was detected.
Asunto(s)
Humanos , Adulto , Persona de Mediana Edad , Femenino , Papillomaviridae/genética , Neoplasias Vaginales/microbiología , ADN Viral , Neoplasias del Cuello Uterino/microbiología , Virus Oncogénicos , Barbados , Carcinoma de Células Escamosas/microbiología , Sondas de ADN de HPV , Adenocarcinoma/microbiología , Reacción en Cadena de la PolimerasaRESUMEN
To evaluate the association between human papillomavirus (HPV) and cervical cancer, we performed a population-based case-control study in Columbia and Spain, the former country having an incidence rate of cervical cancer about 8 times higher than the latter. It included 436 cases of histologically confirmed invasive cervical cancer and 387 randomly selected population controls. Information on demographic variables, sexual behaviour and other risk factors was obtained by interview. HPV-DNA was measured in cervical-swab specimens with 3 hybridization assays: ViraPap, Southern hybridization (SH) and polymerase chain reaction (PCR). The presence of HPV-DNA and detection of types 16, 18, 31, 33 and 35 were strongly associated with cervical cancer in each country regardless of the assay used. For both countries combined the adjusted odds ratios and 95% confidence intervals were: ViraPap OR = 25.9 (10.0-66.7); SH OR = 6.8 (3.4-13.4); and PCR OR = 28.8 (15.7-52.6). HPV-16 was the most common type detected in both cases and controls. Our results indicate that there is a very strong association between HPV 16, 18, 31, 33 and 35 and invasive cervical cancer and that this association is probably causal.
Asunto(s)
Carcinoma de Células Escamosas/etiología , Papillomaviridae/patogenicidad , Infecciones Tumorales por Virus/complicaciones , Neoplasias del Cuello Uterino/etiología , Carcinoma de Células Escamosas/microbiología , Estudios de Casos y Controles , Colombia , ADN Viral/análisis , Femenino , Humanos , Papillomaviridae/genética , Reacción en Cadena de la Polimerasa , España , Neoplasias del Cuello Uterino/microbiologíaAsunto(s)
Neoplasias del Ano/microbiología , Carcinoma de Células Escamosas/microbiología , Papillomaviridae/aislamiento & purificación , Neoplasias del Pene/microbiología , Neoplasias del Cuello Uterino/microbiología , Adulto , Anciano , Neoplasias del Ano/epidemiología , Brasil/epidemiología , Sondas de ADN de HPV , Femenino , Humanos , Hibridación in Situ , Incidencia , Masculino , Persona de Mediana Edad , Neoplasias del Pene/epidemiología , Neoplasias del Cuello Uterino/epidemiologíaRESUMEN
Human papillomavirus type 6 subtype a (HPV-6a) was detected in a human invasive tonsillar carcinoma. Southern blot hybridization analysis showed the presence of additional bands when using non-cutting and single-cut restriction enzymes. Molecular cloning yielded two recombinant clones of 8.0 and 1.4 kb in size. The first represents the complete HPV-6a genome. Sequence analysis of the second clone showed a 0.6 kb DNA sequence corresponding to the L2 region of HPV-6a, whereas the rest belongs to cellular sequences. These data show the presence of a usually low risk HPV type in an invasive carcinoma, at an unusual infection site, with viral DNA integrated into the host genome. These findings add evidence in support of the hypothesis of a relationship between HPV infection and at least some ororespiratory cancers.
Asunto(s)
Carcinoma de Células Escamosas/microbiología , Papillomaviridae/aislamiento & purificación , Neoplasias Tonsilares/microbiología , Infecciones Tumorales por Virus/microbiología , Secuencia de Bases , Southern Blotting , Clonación Molecular , ADN Viral , Femenino , Humanos , Persona de Mediana Edad , Datos de Secuencia Molecular , Mutación , Papillomaviridae/genéticaRESUMEN
An association between anal squamous cell carcinoma and human papillomavirus (HPV) type 16 DNA has been documented in the UK. If HPV type 16 is an important aetiological factor in the development of this tumour it would be expected to occur in anal cancer tissues from other parts of the world. In this study a series of 173 anal squamous cell carcinoma tissue samples from five centres around the world have been examined by DNA hybridisation for HPV type 16 DNA sequences. HPV type 16 DNA was found in 50 of 173 (29%) of these. The prevalence of HPV associated anal squamous cell carcinoma was significantly lower in tissue from India and South Africa than in the Swiss, Polish, or Brazilian samples. HPV associated anal squamous cell carcinoma does occur in other countries and further investigations of the prevalence of these tumours in association with other HPV types are required.