RESUMEN

This study investigated the effect of pulsed electric fields (PEF) pretreatment on rehydration kinetics, firmness, and release of intracellular components of dried chickpeas during rehydration at 35 to 65°C. After soaking preconditioning, chickpeas were subjected to PEF treatments (2.5 and 3.3 kV/cm, 0.2 to 12.0 kJ/kg, 15 µs pulse width, 20 Hz frequency). PEF treated and untreated chickpeas were dried in crossflow air dryer and their rehydration at constant seed/water ratio of 1:5 was studied for 24 hr. During rehydration, moisture, firmness, and concentration of released proteins, carbohydrates and raffinose family oligosaccharides (RFO) were determined and described using appropriate mathematical models. PEF treatment led to up to 70% higher rehydration rates of dried chickpeas. This increase corresponds to rehydration time of approximately 1.5 hr, as opposed to 5 hr for untreated samples. Firmness of PEF treated chickpeas (for energy inputs higher than 3 kJ/kg) during rehydration decreased up to 30% compared to untreated samples. The firmness of untreated samples after 300 min of rehydration was achieved at much shorter times (up to 30 min) for PEF treated samples. At the end of 300 min of rehydration, more than 47.7%, 76.1%, and 86.6% of total raffinose, stachyose, and verbascose, respectively has been extracted, but only 0.03% of nutritionally valuable proteins from PEF treated chickpeas. Consequently, this study demonstrates that PEF processing could be implemented in dried chickpeas processing as pretreatment, for the reduction of rehydration time prior to cooking and of intestinal discomfort caused by RFO.

Asunto(s)

Cicer/química , Cicer/metabolismo , Culinaria/métodos , Carbohidratos de la Dieta/análisis , Proteínas en la Dieta/análisis , Fluidoterapia/métodos , Rafinosa/análisis , Carbohidratos de la Dieta/aislamiento & purificación , Proteínas en la Dieta/aislamiento & purificación , Electricidad , Cinética , Rafinosa/aislamiento & purificación

RESUMEN

A neutral polysaccharide (HJP-1a) and three acid polysaccharides (HJP-2, HJP-3 and HJP-4) were obtained from Z. jujuba cv. Hamidazao. HJP-1a was mainly composed of arabinose and galactose in a ratio of 56.9:20.0, with an average molecular weight of 3.115 × 104 g/mol. HJP-2, HJP-3 and HJP-4 were homogeneous heteropolysaccharides mainly containing galacturonic acid, arabinose and galactose, with average molecular weights of 4.590 × 104, 6.986 × 104 and 1.951 × 105 g/mol, respectively. Structural characterization indicated that the backbone of HJP-3 appeared to be mainly composed of →4)-α-d-GalpA (1→ and →2,4)-α-l-Rhap (1→ residues with some branches consisting of →5)-α-l-Araf (1→ residues and terminals of T-α-l-Araf (1→ and T-ß-d-Galp residues. The four purified fractions displayed dose-dependent radical scavenging activity on ABTS+ radicals and reducing capacity, as well as excellent protective effect on H2O2-induced HepG2 cells and metronidazole-damaged zebrafish embryos, especially HJP-2 in vitro and HJP-1a in vivo. Therefore, the polysaccharides from Z. jujuba cv. Hamidazao could be used as a potential antioxidant in functional foods.

Asunto(s)

Antioxidantes , Polisacáridos , Ziziphus/química , Ácidos/química , Ácidos/farmacología , Animales , Antioxidantes/química , Antioxidantes/farmacología , Carbohidratos de la Dieta/análisis , Carbohidratos de la Dieta/aislamiento & purificación , Carbohidratos de la Dieta/farmacología , Femenino , Células Hep G2 , Humanos , Concentración de Iones de Hidrógeno , Masculino , Estructura Molecular , Extractos Vegetales/química , Extractos Vegetales/farmacología , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Pez Cebra

RESUMEN

A 91 kDa heteropolysaccharide (F2) was isolated from Mangifera indica fruit via extraction with H2O, purification by C2H5OH, starch removal and ion exchange chromatography. This polymer was made up mostly of Ara, Gal, Glc, Rha, Xyl, and GalA in a 37: 29: 9:3:2:19 molar proportion. It inherited a small backbone containing GalpA and Rhap units substituted with very large side chains containing differently linked Ara and Gal units plus esterified gallic acid (GA) residue. Several enzymes generated oligosaccharides including (i) Ara2-10Ac6-22, (ii) Gal1-8Ac5-26 and (iii) GA1Gal1Ac7 were characterized. This polysaccharide, which showed dose dependent antioxidant activity, exhibited synergism with gallic acid, and formed a complex (K = 1.2 × 106 M-1) with ß-lactoglobulin. Accordingly, H2O treatment produces a polysaccharide with desired biochemical properties; this could be effective in designing innovative functional food with flexible makeup.

Asunto(s)

Antioxidantes/química , Lactoglobulinas/química , Mangifera/química , Polisacáridos/química , Antioxidantes/aislamiento & purificación , Secuencia de Carbohidratos/genética , Carbohidratos de la Dieta/aislamiento & purificación , Frutas/química , Frutas/genética , Humanos , Lactoglobulinas/genética , Mangifera/genética , Monosacáridos/química , Monosacáridos/genética , Monosacáridos/aislamiento & purificación , Oligosacáridos/química , Oligosacáridos/genética , Oligosacáridos/aislamiento & purificación , Pectinas/química , Pectinas/genética , Polisacáridos/genética , Polisacáridos/aislamiento & purificación

RESUMEN

Abelmoschus esculentus L (okra) is widely used as a healthy vegetable and favourable source of dietary medicine. Okra flowers which are by-products of okra, are rich in polysaccharide, polyphenols and trace elements etc., however, except a few for health tea, most of them were discarded as the waste of resources. In this study, a polysaccharide named AEFP22 was extracted, purified and identified from okra flowers, and its physicochemical property and antioxidant activity were also elucidated. AEFP22, with a molecular weight of 2.741 × 105 Da, was composed of Rha, GalA and Gal in the ratio of 1: 1.02: 0.86. The methylation and nuclear magnetic resonance (NMR) analysis indicated AEFP22 was composed of [2)-α-D-Rhap-(1 â†’ 4)-α-D-GalpA-(1 â†’ 2,4)-α-D-Rhap-(1 â†’ 4)-α-D-GalpA-(1] with branch of terminal T-α-D-Galp pointed at C4 of 1,2,4-α-D-Rhap. The Conge-red test, Atomic force microscope (AFM) and Scanning electron microscope (SEM) further revealed the triple-helical conformation, irregular sheet structure with molecule aggregations of AEFP22. The physicochemical property analysis indicated AEFP22 possessed stable thermal property and exhibited shear-thinning and normal Newtonian fluid in different concentrations, -7.04 mV zeta potential and polymerization phenomenon existed in AEFP22 solution. AEFP22 exhibited good 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging ability. These results indicated potential utilization of AEFP22 in nutritional food and material application.

Asunto(s)

Abelmoschus/química , Antioxidantes/metabolismo , Flores/química , Polifenoles/análisis , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Carbohidratos de la Dieta/aislamiento & purificación , Carbohidratos de la Dieta/metabolismo , Peso Molecular , Polisacáridos/metabolismo

RESUMEN

This study aimed to explore the effects of Astragalus membranaceus polysaccharide (AMP) on the growth and innate immunity of crucian carp (Carassius auratus). Crucian carps were randomly divided into a control group (fed with basal diet) and three AMP groups (received basal diet supplemented with 50, 100 and 150 mg/kg AMP). After 60 days of culture, the crucian carps from each group were weighed, and their immune indexes were measured. Another batch crucian carps from each group was injected with 0.15 ml of 107 CFU/ml Aeromonas hydrophila. The body weight gain, feed conversion rate, specific growth rate and digestive enzyme activity of the crucian carps in the low and middle doses of AMP groups were higher than those in the control group. The AMP groups had significantly higher survival rate and alkaline phosphatase level but lower glutamic-oxaloacetic transaminase, glutamic-alanine transaminase and serum bacteria number compared with the control group. The optimal dose of dietary AMP required for the maximum growth of crucian carp was 100 mg/kg. These results showed that AMP could promote the growth of crucian carps, improve their disease resistance and thus may be developed as a dietary supplement.

Asunto(s)

Astragalus propinquus/química , Carpas/crecimiento & desarrollo , Carpas/inmunología , Carbohidratos de la Dieta/farmacología , Inmunidad Innata/efectos de los fármacos , Polisacáridos/química , Polisacáridos/farmacología , Animales , Cromatografía Líquida de Alta Presión , Carbohidratos de la Dieta/aislamiento & purificación , Polisacáridos/aislamiento & purificación

RESUMEN

Sargassum fusiforme polysaccharides, acidic water-soluble polysaccharides extract from Sargassum fusiforme, are mainly composed of alginic acid, fucoidan and laminaran. Alginic acid is carboxyl-containing polysaccharide formed by joining ß-D-mannuronic acid and α-L-guluronic acid through ß-(1→4)/α-(1→4) glycosidic bond. Fucoidan, a natural water-soluble sulfated heteropolysaccharide with fucose and sulfuric acid groups as the core structure, is mainly linked by L-fucose through α-(1→3) glycosidic bond and has the strongest biological activity. Laminaran is mainly composed of ß-D-glucose through ß-(1→3) glycosidic bond linkage. Sargassum fusiforme polysaccharides have a variety of pharmacological activities, including antioxidant, anti-tumor, promoting immunity, anti-aging, prompting bone growth, lowering blood glucose, anti-coagulation, anti-virus, anti-bacteria, anti-fatigue, promoting growth and development, and skin protection. These activities are closely related to the functions of fucoidan in Sargassum fusiforme polysaccharides, which fucoidan is able to strengthen immune system and antioxidation in human body. In this review, the composition, the isolation and purification, and the biological activities of Sargassum fusiforme polysaccharides are discussed and can bereference for further study.

Asunto(s)

Ácido Algínico , Glucanos , Polisacáridos , Sargassum/metabolismo , Envejecimiento/efectos de los fármacos , Ácido Algínico/química , Ácido Algínico/aislamiento & purificación , Ácido Algínico/farmacología , Animales , Bacterias/efectos de los fármacos , Línea Celular , Carbohidratos de la Dieta/aislamiento & purificación , Carbohidratos de la Dieta/farmacología , Glucanos/química , Glucanos/aislamiento & purificación , Glucanos/farmacología , Humanos , Hipoglucemiantes/aislamiento & purificación , Hipoglucemiantes/farmacología , Ratones , Neoplasias/tratamiento farmacológico , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Ratas , Virus/efectos de los fármacos

RESUMEN

The aim of this study was to evaluate Egyptian date palm pollen (DPP) grains composition, physical and functional potentials in comparing with two forms; 80% ethanol extract, and nanoencapsulated form. Functional yoghurt fortified with DPP in three forms was prepared and their physicochemical, microstructure, texture and sensory characteristics were assessed. The micro morphology was explored via Scanning Electron Microscope (SEM). Fourier Transform Infrared (FTIR) spectroscopy was employed for functional groups detection. Phenolic compounds were detected by High Performance Liquid Chromatography (HPLC) while fatty acids were identified via Gas Liquid Chromatography (GLC). Cytotoxicity of DPP nanocapsules was evaluated against RPE1 cell line (BJ1). The Egyptian date palm pollen grains evaluation revealed its rich content of protein and carbohydrate (36.28 and 17.14 g/ 100g), high content of Fe, Zn and Mg (226.5, 124.4 and 318 mg/100g), unsaturated fatty acids ω-3, ω-6 and ω-9 (8.76, 20.26 and 7.11 g/100g, which was increased by ethanol extraction) and phenolic compounds especially catechin (191.73 µg/mL) which was pronounced in DPP antioxidant potentials (IC50 35.54 mg/g). The FTIR analyses indicated the presence of soluble amides (proteins) and polysaccharides (fibers) functional groups in DPP. Fortification with nanoencapsulated DPP proved to be safe and the recommended form due to the announced positive characteristics. Yoghurt fortification with DPP forms enhanced viscosity, syneresis and Water Holding Capacity (WHC), which can be considered a symbiotic functional product as it contained both probiotics (106 CFU/g) and prebiotics represented in DPP forms.

Asunto(s)

Composición de Medicamentos/métodos , Alimentos Funcionales/análisis , Phoeniceae/química , Polen/química , Yogur/análisis , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Línea Celular , Supervivencia Celular/efectos de los fármacos , Carbohidratos de la Dieta/aislamiento & purificación , Células Epiteliales/citología , Células Epiteliales/efectos de los fármacos , Ácidos Grasos Insaturados/química , Ácidos Grasos Insaturados/aislamiento & purificación , Humanos , Hierro/análisis , Magnesio/análisis , Nanoestructuras/química , Nanoestructuras/ultraestructura , Fenoles/química , Fenoles/aislamiento & purificación , Extractos Vegetales/química , Proteínas de Plantas/química , Proteínas de Plantas/aislamiento & purificación , Epitelio Pigmentado de la Retina/citología , Epitelio Pigmentado de la Retina/efectos de los fármacos , Zinc/análisis

RESUMEN

BACKGROUND: The aim of this study was to compare the effects of carbohydrate (CHO) drinks (6% per volume) sweetened with maple (syrup or sap) to a commercial sports drink, glucose, and a control solution (water) on cognitive flexibility during high-intensity intermittent exercise. METHODS: Eighty-five active men completed six 3-min bouts at 95% of their maximal aerobic power on a stationary bike, with 3 min of passive rest between efforts. Subjects were randomly allocated to an ingestion condition. Following each exercise bout, subjects ingested 166 mL of the experimental solution, drinking a total of 1 L of the same solution throughout the experimentation. Cognitive flexibility was measured using reaction time and accuracy on the Stroop task. The cognitive task was performed a total of 10 times, including 15 and 30 min post-exercise. Glycemia and cerebral oxygenation were also measured at each time point. Statistical analyses were performed using a two-way ANOVA (Condition × Time) with repeated measures. RESULTS: The ingestion of maple products and the commercial sports drink led to a lesser increase in glycemia than glucose ingestion. CHO ingestion, when compared to water, induced a slight reduction in reaction times on the cognitive task, especially in the switching trials. CHO ingestion had no impact on cerebral oxygenation. CONCLUSIONS: This study shows that CHO ingestion, regardless of its type, tends to improve cognitive performance throughout exercise, especially during difficult cognitive tasks.

Asunto(s)

Acer/química , Bebidas , Encéfalo/irrigación sanguínea , Circulación Cerebrovascular , Cognición , Carbohidratos de la Dieta/administración & dosificación , Entrenamiento de Intervalos de Alta Intensidad , Consumo de Oxígeno , Oxígeno/sangre , Exudados de Plantas/administración & dosificación , Adulto , Glucemia/metabolismo , Carbohidratos de la Dieta/sangre , Carbohidratos de la Dieta/aislamiento & purificación , Humanos , Masculino , Exudados de Plantas/sangre , Exudados de Plantas/aislamiento & purificación , Factores de Tiempo , Adulto Joven

RESUMEN

A new purified polysaccharide (PNP40c-1) with a molecular weight of 2.06 × 105 Da was obtained from pine nuts (Pinus koraiensis Sieb. et Zucc.). Structural analysis indicated that PNP40c-1 is a homogeneous heteropolysaccharide composed of arabinose, rhamnose and glucose in a molar ratio of 2.98:1.00:0.52. The major backbone consisted of →3,4)-α-l-Arap(1→, →4)-α-l-Arap3Me(1→, →3)-α-l-Rhap(1→ and →6)-ß-d-Glcp(1→, and the side chain is ß-d-Glcp-(1→ linked at C4-position of →3,4)-α-l-Arap(1→. In addition, the hepatoprotective effect of PNP40c-1 was investigated by human hepatocyte cell line L02 treated with carbon tetrachloride (CCl4). Results suggested that PNP40c-1 could protect L02 cells from CCl4-induced damage by enhancing the activities of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), increasing the level of non-enzymatic antioxidant glutathione (GSH), suppressing lipid perioxidation and further reducing the leakage of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Hence, PNP40c-1 could be a promising functional food to serve as hepatoprotective agent.

Asunto(s)

Carbohidratos de la Dieta/farmacología , Nueces/química , Pinus/química , Polisacáridos/farmacología , Sustancias Protectoras/farmacología , Alanina Transaminasa/metabolismo , Aspartato Aminotransferasas/metabolismo , Secuencia de Carbohidratos , Tetracloruro de Carbono , Línea Celular Tumoral , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Carbohidratos de la Dieta/aislamiento & purificación , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Peroxidación de Lípido/efectos de los fármacos , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Sustancias Protectoras/química , Sustancias Protectoras/aislamiento & purificación , Superóxido Dismutasa/metabolismo

RESUMEN

Our previous study characterized the structure-associated immunomodulatory effects of an edible Dendrobium aphyllum polysaccharide (DAP), and the in vitro gastrointestinal digestions highlighted DAP could be digested by the GI tract in some extent. Therefore, the present study further explored the digestive properties in vivo to infer the metabolic pathway with health mice model. Results revealed that DAP-treated group showed slightly lower blood glucose levels and significantly higher (P < 0.05) enzyme activities, namely G6Pase and GDH with an increment of about 0.4 to 0.9 and 45 to 91 U/mL, respectively. Meanwhile, DAP up-regulated the expression of glucose transporters, GLUT1 and GLUT2 in the increment rates of 56.34% to 68.28% and 76.63% to 83.03%, in colon. Furthermore, the beneficial effects of DAP on colon were confirmed by the increment of four types short chain fatty acids and the health-promoting microbiota diversity. The above results successfully identify the metabolic pathways after the oral administration of bioactive DAP. PRACTICAL APPLICATION: The metabolic pathways of Dendrobium aphyllum polysaccharide, after artificially stimulated oral administration, were characterized. The most of the unabsorbed portion of DAP were utilized by the colon microbiota, resulting in the significantly increasing production of four health-promoting SCFAs. The unabsorbed portion of DAP upregulated the diversity of various beneficial microbiota genus, and meanwhile downregulated kinds of harmful microbiota genus.

Asunto(s)

Dendrobium/química , Carbohidratos de la Dieta , Extractos Vegetales , Polisacáridos , Animales , Carbohidratos de la Dieta/aislamiento & purificación , Carbohidratos de la Dieta/metabolismo , Microbioma Gastrointestinal/fisiología , Redes y Vías Metabólicas , Ratones , Extractos Vegetales/aislamiento & purificación , Extractos Vegetales/metabolismo , Polisacáridos/aislamiento & purificación , Polisacáridos/metabolismo

RESUMEN

The conditions of wheat germ polysaccharide extraction were determined using the water extraction method. The gastric and intestinal digestion of wheat germ polysaccharide was analyzed in vitro. According to a single factor experiment and response surface experiment, the optimal extraction conditions of wheat germ polysaccharide were the following: liquid-solid ratio, 5:1 mL/g; extraction temperature, 69 °C; repetition of the extracting procedure, 3 times; extraction time, 44 min. Under such conditions, wheat germ polysaccharide yield was 8.89% ±â€¯0.002%. In the in vitro gastrointestinal experiment, wheat germ polysaccharide was digested and the content of reducing sugar increased during the digestion period, indicating that this increase might be due to the breakdown of glycosidic bonds in the wheat germ polysaccharide. Furthermore, no monosaccharide was detected, demonstrating that the gastrointestinal digestion did not cause free monosaccharide released. These results show some preliminary characteristics of the wheat germ polysaccharide in vitro digestion, providing a theoretical basis for further understanding wheat germ polysaccharide properties.

Asunto(s)

Carbohidratos de la Dieta/aislamiento & purificación , Grano Comestible/química , Polisacáridos/aislamiento & purificación , Triticum/química , Digestión/efectos de los fármacos , Medicamentos Herbarios Chinos/química , Células Germinativas de las Plantas , Calor , Humanos , Mucosa Intestinal/metabolismo , Polisacáridos/química , Agua/química

RESUMEN

Blackcurrants (Ribes nigrum L.) have various benefits for human health. In particular, a polysaccharide derived from blackcurrant was found to be an immunostimulating food ingredient in a mouse model. We named a polysaccharide derived from blackcurrant cassis polysaccharide (CAPS). In a previous clinical study, we reported that CAPS affects skin dehydration, demonstrating its effectiveness against skin inflammation was related to atopic dermatitis; skin inflammation caused skin dehydration. However, there are no studies regarding CAPS effectiveness against skin dehydration. The current study aimed to investigate CAPS effectiveness against skin dehydration. We further demonstrate the effect of oral administration of CAPS on skin dehydration caused by ultraviolet (UV) irradiation-induced inflammation in mice. We found that CAPS administration suppresses skin dehydration caused by UV irradiation. We also found that CAPS decreases interleukin-6 and matrix metalloproteinase transcription levels in the mouse skin. These results show that CAPS improves skin hydration in UV-irradiated mice.

Asunto(s)

Antiinflamatorios no Esteroideos/uso terapéutico , Dermatitis Atópica/terapia , Carbohidratos de la Dieta/uso terapéutico , Frutas/química , Extractos Vegetales/uso terapéutico , Ribes/química , Piel/metabolismo , Animales , Antiinflamatorios no Esteroideos/administración & dosificación , Antiinflamatorios no Esteroideos/química , Antiinflamatorios no Esteroideos/aislamiento & purificación , Dermatitis Atópica/etiología , Dermatitis Atópica/inmunología , Dermatitis Atópica/metabolismo , Carbohidratos de la Dieta/administración & dosificación , Carbohidratos de la Dieta/análisis , Carbohidratos de la Dieta/aislamiento & purificación , Fibras de la Dieta/administración & dosificación , Fibras de la Dieta/análisis , Fibras de la Dieta/uso terapéutico , Suplementos Dietéticos/análisis , Femenino , Regulación de la Expresión Génica/efectos de la radiación , Interleucina-6/antagonistas & inhibidores , Interleucina-6/genética , Interleucina-6/metabolismo , Metaloproteinasa 13 de la Matriz/química , Metaloproteinasa 13 de la Matriz/genética , Metaloproteinasa 13 de la Matriz/metabolismo , Ratones Pelados , Extractos Vegetales/administración & dosificación , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Prebióticos/administración & dosificación , Prebióticos/análisis , Traumatismos Experimentales por Radiación/inmunología , Traumatismos Experimentales por Radiación/metabolismo , Traumatismos Experimentales por Radiación/terapia , Piel/inmunología , Piel/efectos de la radiación , Organismos Libres de Patógenos Específicos , Rayos Ultravioleta/efectos adversos , Agua/metabolismo

RESUMEN

A novel bioactive polysaccharide, GFP-22, was isolated from the fruit bodies of Grifola frondosa by anion-exchange and gel filtration chromatography. Structure of GFP-22 was investigated using gas chromatography-mass spectrometry (GC-MS), methylation, nuclear magnetic resonance (NMR), high-performance size exclusion chromatography-multi-angle laser light scattering-refractive index detector (HPSEC-MALLS-RI) and atomic force microscopy (AFM) analysis. The backbone of GFP-22 is composed of 1,4-ß-d-Glcp, 1,3-ß-d-Glcp, 1,6-α-d-Glcp, 1,6-α-d-Galp, 1,4,6-α-d-Manp and 1,3,6-α-d-Manp units. Molecular weight of GFP-22 is 2.72 × 104 Da. GFP-22 has a linear filamentous structure. The administration of GFP-22 could improve or reverse the CTX-induced immunosuppression, significantly enhance the spleen and thymus indices, spleen lymphocyte proliferation and cytokines production in splenocytes. These findings suggest that GFP-22 could be explored as a natural and potential immunomodulatory agent and as an alternative means of lessening chemotherapy-induced immunosuppression.

Asunto(s)

Proliferación Celular/efectos de los fármacos , Grifola/química , Factores Inmunológicos/química , Polisacáridos/química , Animales , Cromatografía en Gel , Citocinas/genética , Carbohidratos de la Dieta/aislamiento & purificación , Humanos , Factores Inmunológicos/aislamiento & purificación , Espectroscopía de Resonancia Magnética , Ratones , Peso Molecular , Polisacáridos/aislamiento & purificación , Células RAW 264.7/efectos de los fármacos

RESUMEN

Polysaccharides are food ingredients that critically determine rheological properties and shelf life. A qualitative and quantitative assessment on food-specific polysaccharide mixtures by 1H NMR is presented. The method is based on the identification of intact polysaccharides, combined with a quantitative analysis of their monosaccharide constituents. Identification of the polysaccharides is achieved by 1H NMR line shape fitting with pure compound spectra. The monomeric composition was determined using the Saeman hydrolysis procedure, followed by direct monosaccharide quantification by 1H NMR. In the quantification, both the monosaccharide degradation during hydrolysis, as well as a correction for the non-instantaneous polysaccharide dissolution were taken into account. These factors were particularly important for the quantification of pectins. The method showed overall good repeatability (RSDr=4.1±0.9%) and within-laboratory reproducibility (RSDR=6.1±1.4%) for various food polysaccharides. Polysaccharide mixtures were quantitatively resolved by a non-negative least squares estimation, using identified polysaccharides and their molar monosaccharide stoichiometry as prior knowledge. The accuracy and precision of the presented method make it applicable to a wide range of food polysaccharide mixtures with complex and overlapping 1H NMR spectra.

Asunto(s)

Carbohidratos de la Dieta/análisis , Monosacáridos/análisis , Monosacáridos/química , Resonancia Magnética Nuclear Biomolecular/métodos , Carbohidratos de la Dieta/aislamiento & purificación , Industria de Alimentos , Hidrólisis , Análisis de los Mínimos Cuadrados , Peso Molecular , Pectinas/análisis , Reproducibilidad de los Resultados , Agua/química

RESUMEN

Spirulina platensis is considered an alternative and excellent source of protein [46-63% dry basis (DB)], having protein levels comparable to meat and soybeans. Thus, it can be considered an adequate ingredient to supply the necessity of this compound in the food industry. Its carbohydrates (8-14% DB) may also be a useful food ingredient or a potential source of bioenergy. Thus, extracting these compounds from the microalgae biomass will maximize its exploitation. Sonication can completely or partially degrade the microalgal cell wall, providing a useful technique to extract the protein and carbohydrate. This study used a sequential strategy of experimental design (fractional factorial design and central composite rotatable design) to evaluate the protein and carbohydrate extraction from S. platensis defatted biomass using ultrasonic waves and mechanical agitation, under alkaline conditions. The optimal conditions for protein and carbohydrate co-extraction were established by selecting and maximizing the variables that significantly influenced the extraction. The optimized percentages recovery from the extraction process yielded 75.76% protein and 41.52% carbohydrate at 33-40min sonication and 40-55min agitation. The protein fraction may be further concentrated and purified for use in food formulations, and the carbohydrates may be a useful feedstock for bioethanol production.

Asunto(s)

Proteínas Bacterianas/aislamiento & purificación , Carbohidratos de la Dieta/aislamiento & purificación , Proteínas en la Dieta/aislamiento & purificación , Suplementos Dietéticos , Aditivos Alimentarios/aislamiento & purificación , Manipulación de Alimentos/métodos , Microbiología Industrial/métodos , Microalgas/metabolismo , Sonicación , Spirulina/metabolismo , Ultrasonido/métodos , Biomasa , Microalgas/crecimiento & desarrollo , Spirulina/crecimiento & desarrollo

RESUMEN

The two fractions of polysaccharide MPS-1 and MPS-2 were extracted from Lepidium meyenii Walp. (maca) by water, and purified using a DEAE-52 and a Sephadex G-100 column. The molecular weight (MW) of MPS-1 was 7.6kDa, and the MW of MPS-2 was 6.7kDa. The MPS-1 was composed of xylose, arabinose, galactose and glucose, with the mole ratio 1:1.7:3.3:30.5; the MPS-2 was composed of arabinose, galactose and glucose, with the mole ratio 1:1.3:36.8. The IR spectrum implied that only α-pyranose existed in MPS-1, and both α-pyranose and ß-pyranose existed in MPS-2. The anti-fatigue activities of MPS-1 and MPS-2 were measured by the forced swimming test, along with the determination of blood lactate (BLA), urea nitrogen (BUN), lactic dehydrogenase (LDH) activity and liver glycogen (LG). The results indicated that both MPS-1 and MPS-2 presented dose-dependently positive effects on the fatigue related parameters. Additionally, MPS-2 has a better anti-fatigue effect than MPS-1.

Asunto(s)

Carbohidratos de la Dieta/farmacología , Metabolismo Energético/efectos de los fármacos , Fatiga/tratamiento farmacológico , Lepidium/química , Polisacáridos/farmacología , Animales , Arabinosa/análisis , Nitrógeno de la Urea Sanguínea , Fraccionamiento Químico/métodos , Carbohidratos de la Dieta/aislamiento & purificación , Relación Dosis-Respuesta a Droga , Fatiga/metabolismo , Fatiga/fisiopatología , Galactosa/análisis , Glucosa/análisis , Glucógeno/metabolismo , Hidrólisis , L-Lactato Deshidrogenasa/sangre , Ácido Láctico/sangre , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Ratones , Peso Molecular , Extractos Vegetales/química , Polisacáridos/química , Polisacáridos/aislamiento & purificación , Natación , Xilosa/análisis

RESUMEN

CE applications to charged polysaccharides are briefly reported. A simple procedure is presented to determine the esterification degree of a hyaluronan derivative. In this case the degree of substitution was as low as 14 %.The molecular weight distribution of mannuronic oligosaccharides mixture produced by hydrolysis of native polymannuronic is readily calculated from peak area of the species resolved by CE on the basis of a specific degree of polymerization.The influence of the applied electric field strength on the free solution mobility of hyaluronan samples is briefly addressed for molar masses of the order of 10(5) and 10(6) g/mol. The data are compared with the results obtained for a 50 % galactose substituted HA.Mobility data obtained as a function of buffer pH for a native HA sample as well as for two galactose-amide HA derivatives, having slightly different degrees of substitution, are presented and discussed in terms of the polymer charge density parameters ξ.In most cases, more questions than answers arise from the application of CE to charged polysaccharides. However, perspectives are disclosed for a further understanding of the reliability of CE applied for the structural elucidation of such macromolecules.

Asunto(s)

Carbohidratos de la Dieta/aislamiento & purificación , Electroforesis Capilar/métodos , Ácido Hialurónico/aislamiento & purificación , Polisacáridos/aislamiento & purificación , Esterificación , Ácido Hialurónico/química , Peso Molecular , Polisacáridos/química

RESUMEN

Dragon fruit is a tropical fruit with good taste. It can bring health benefits to human body. As one of the major bioactive components in this fruit, the polysaccharides might contribute to the health benefits. However, the precise structure information remains unknown. A leading polysaccharide of dragon fruit pulp, DFPP, was purified and identified by NMR and GC-MS. →4-ß-d-GlcpA-1→, →6-ß-d-Galp-1→ and →4-α-l-Rhap-1→ constituted the backbone and α-l-Araf-1→5-α-l-Araf-1→ formed the branch chain. The precise structure was putatively identified as below. The molecular weight was 2.2×10(3)kDa. The structure information of polysaccharides will be helpful to understand this fruit.

Asunto(s)

Caryophyllales/química , Extractos Vegetales/química , Polisacáridos/química , Secuencia de Carbohidratos , Carbohidratos de la Dieta/aislamiento & purificación , Frutas/química , Espectroscopía de Resonancia Magnética , Estructura Molecular , Extractos Vegetales/aislamiento & purificación , Polisacáridos/aislamiento & purificación , Agua/química

RESUMEN

Soy protein is a well-known nutritional supplement in proteinaceous food and animal feed. However, soybeans contain complex carbohydrate. Selective carbohydrate removal by enzymes could increase the protein content and remove the indigestibility of soy products for inclusion in animal feed. Complete hydrolysis of soy flour carbohydrates is challenging due to the presence of proteins and different types of non-structural polysaccharides. This study is designed to guide complex enzyme mixture required for hydrolysis of all types of soy flour carbohydrates. Enzyme broths from Aspergillus niger, Aspergillus aculeatus and Trichoderma reesei fermentations were evaluated in this study for soy carbohydrate hydrolysis. The resultant hydrolysate was measured for solubilized carbohydrate by both total carbohydrate and reducing sugar analyses. Conversion data attained after 48h hydrolysis were first fitted with models to determine the maximum fractions of carbohydrate hydrolyzable by each enzyme group, i.e., cellulase, xylanase, pectinase and α-galactosidase. Kinetic models were then developed to describe the increasing conversions over time under different enzyme activities and process conditions. The models showed high fidelity in predicting soy carbohydrate hydrolysis over broad ranges of soy flour loading (5-25%) and enzyme activities: per g soy flour, cellulase, 0.04-30 FPU; xylanase, 3.5-618U; pectinase, 0.03-120U; and α-galactosidase, 0.01-60U. The models are valuable in guiding the development and production of optimal enzyme mixtures toward hydrolysis of all types of carbohydrates present in soy flour and in optimizing the design and operation of hydrolysis reactor and process.

Asunto(s)

Carbohidratos de la Dieta/aislamiento & purificación , Proteínas de Vegetales Comestibles/aislamiento & purificación , Alimentos de Soja/análisis , Proteínas de Soja/aislamiento & purificación , Alimentación Animal/análisis , Animales , Aspergillus/enzimología , Fermentación , Humanos , Hidrólisis , Cinética , Modelos Biológicos , Trichoderma/enzimología

RESUMEN

A polysaccharide fraction (FMPS) was isolated from floral mushrooms cultivated in Huangshan Mountain, and the rheological properties of FMPS in aqueous solutions were investigated. The FMPS solution showed shear-thinning behavior at 25°C. Dynamic viscoelastic tests revealed that G' and G″ exhibited strong dependences on the concentration and temperature. The FMPS/water system exhibited sol and weak gel behavior with the change of concentration and temperature. The exponent n of G'∼ω(n) and tan δ also exhibited strong dependences on the concentration and temperature. The gel point (cgel) of FMPS solution was 1.16×10(-2)g/mL at 15°C, and the Tgel of 1.4×10(-2)g/mL FMPS solution was 20.6°C. Dynamic frequency sweep measurements indicated that the FMPS gel system was stable in the selected range of frequency. The heating-cooling process proved that the sol-gel transition of FMPS in aqueous solutions was thermally reversible.

Asunto(s)

Agaricales/química , Polisacáridos/química , China , Carbohidratos de la Dieta/aislamiento & purificación , Elasticidad , Polisacáridos/aislamiento & purificación , Reología , Soluciones , Estrés Mecánico , Temperatura , Viscosidad