RESUMEN
The aim of this study was to evaluate the anti-edematogenic activity of X. americana L. (HEXA) hydroethanolic extract in ear edema models (acute and chronic) induced by croton oil and by different phlogistic agents (arachidonic acid, capsaicin, phenol and histamine), identifying the possible anti-edematogenic mechanism. HEXA demonstrated a significant anti-edematogenic effect at concentrations of 100-500⯵g/ear in ear edema induced by croton oil with higher inhibition of edema of 39.37. However, the concentrations of 100 and 200⯵g/ear were taken as a standard, demonstrating the effect in the chronic model induced by croton oil with inhibition of 61.62% and 48.74%. In the AA-induced ear edema model, HEXA showed inhibition of: 24.45% and 32.31%; capsaicin inhibition of 72.72% and 47.57%; phenol inhibition of 34% and 20.1%; and histamine inhibition of 31.8% and 21.62%. Then, the results were showed that HEXA demonstrated an anti-edematogenic effect in acute and chronic inflammation models, demonstrating a probable mechanism of action by the inhibition or modulation of key mediators of the inflammatory process. The chemical profile and presence of flavonoids guaranteeing a profile of activity similar to natural drugs that act or modulate the production of mediators of inflammations.
Asunto(s)
Cromatografía Líquida de Alta Presión/métodos , Dermatitis/tratamiento farmacológico , Edema/tratamiento farmacológico , Olacaceae/química , Extractos Vegetales/uso terapéutico , Animales , Ácido Araquidónico/efectos adversos , Ácido Araquidónico/antagonistas & inhibidores , Capsaicina/efectos adversos , Capsaicina/antagonistas & inhibidores , Aceite de Crotón/toxicidad , Relación Dosis-Respuesta a Droga , Edema/inducido químicamente , Femenino , Histamina/efectos adversos , Antagonistas de los Receptores Histamínicos/uso terapéutico , Ratones , Fenol/efectos adversos , Fenol/antagonistas & inhibidoresRESUMEN
During a migraine attack capsaicin-sensitive trigeminal sensory nerves release calcitonin gene-related peptide (CGRP), producing cranial vasodilatation and central nociception; hence, trigeminal inhibition may prevent this vasodilatation and abort migraine headache. This study investigated the role of spinal α2-adrenoceptors and their subtypes (i.e. α(2A), α(2B) and/or α(2C)-adrenoceptors) in the inhibition of the canine external carotid vasodilator responses to capsaicin. Anaesthetized vagosympathectomized dogs were prepared to measure arterial blood pressure, heart rate and external carotid conductance. The thyroid artery was cannulated for one-min intracarotid infusions of capsaicin, α-CGRP and acetylcholine. A cannula was inserted intrathecally for spinal (C1-C3) administration of 2-amino-6-ethyl-4,5,7,8-tetrahydro-6H-oxazolo-[5,4-d]-azepin-dihydrochloride (B-HT 933; a selective α2-adrenoceptor agonist) and/or the α2-adrenoceptor antagonists rauwolscine (α(2A/2B/2C)), 2-[(4,5-dihydro-1H-imidazol-2-yl)methyl]-2,3-dihydro-1-methyl-1H-isoindole maleate (BRL44408; α(2A)), imiloxan (α(2B)) or acridin-9-yl-[4-(4-methylpiperazin-1-yl)-phenyl]amine (JP-1302; α(2C)). Infusions of capsaicin, α-CGRP and acetylcholine dose-dependently increased the external carotid conductance. Intrathecal B-HT 933 (1000 and 3100 µg) inhibited the vasodilator responses to capsaicin, but not those to α-CGRP or acetylcholine. This inhibition, abolished by rauwolscine (310 µg), was: (i) unaffected by 3,100 µg imiloxan; (ii) partially blocked by 310 µg of BRL44408 or 100 µg of JP-1302; and (iii) abolished by 1,000 µg of BRL44408 or 310 µg of JP-1302. Thus, intrathecal B-HT 933 inhibited the external carotid vasodilator responses to capsaicin. This response, mediated by spinal α2-adrenoceptors unrelated to the α(2B)-adrenoceptor subtype, resembles the pharmacological profile of α(2C)-adrenoceptors and, to a lesser extent, α(2A)-adrenoceptors.
Asunto(s)
Antagonistas de Receptores Adrenérgicos alfa 2/uso terapéutico , Arteria Carótida Externa/efectos de los fármacos , Modelos Animales de Enfermedad , Trastornos Migrañosos/tratamiento farmacológico , Receptores Adrenérgicos alfa 2/metabolismo , Médula Espinal/efectos de los fármacos , Vasoconstrictores/uso terapéutico , Acetilcolina/metabolismo , Antagonistas de Receptores Adrenérgicos alfa 2/administración & dosificación , Antagonistas de Receptores Adrenérgicos alfa 2/química , Analgésicos no Narcóticos/administración & dosificación , Analgésicos no Narcóticos/antagonistas & inhibidores , Analgésicos no Narcóticos/uso terapéutico , Animales , Péptido Relacionado con Gen de Calcitonina/metabolismo , Capsaicina/antagonistas & inhibidores , Capsaicina/toxicidad , Arteria Carótida Externa/fisiología , Vértebras Cervicales , Perros , Hemodinámica/efectos de los fármacos , Infusión Espinal , Masculino , Trastornos Migrañosos/inducido químicamente , Trastornos Migrañosos/metabolismo , Isoformas de Proteínas/antagonistas & inhibidores , Isoformas de Proteínas/metabolismo , Receptores Adrenérgicos alfa 2/química , Flujo Sanguíneo Regional/efectos de los fármacos , Médula Espinal/metabolismo , Vasoconstrictores/administración & dosificación , Vasoconstrictores/antagonistas & inhibidores , Vasodilatadores/antagonistas & inhibidores , Vasodilatadores/toxicidadRESUMEN
Animal models for mechanical pressure or heat nociception usually only measure the threshold response latency. In this study, the effect of typical sensitising treatments on the lasting nocifensive behaviour elicited after a supra-threshold heating stimulus - the hyperpathic component of hypernociception - was assessed. Male Wistar rats received either intra-plantar (i.pl.) injection of 350ng PGE(2) (50microL) or topical application (t.a.) of 100% dimethylsulfoxide (DMSO), and 10mM capsaicin. One hour after the paw treatments the number of nocifensive events (NNE) was scored hourly (6h), for 5min, immediately after a hind paw immersion in hot water (50 degrees C/7s). PGE(2), DMSO and capsaicin increased the NNE -induced by the supra-threshold stimuli. Indomethacin (2.5mg/kg i.p.), given 30min before paw treatments, completely inhibited NNE in all groups (P<0.01). However, indomethacin given 60min after PGE(2) did not reverse this sensitisation. PGE(2) and DMSO did not lower the heat threshold in the paw withdrawal test, although carrageenan and capsaicin were effective (P<0.05). Capsaicin neonatal treatment (CNT) (50mg/kg) reduced the sensitisation induced by DMSO and capsaicin (P<0.01), but not that induced by PGE(2). These data suggest that the heat-induced lasting nociception is probably conveyed by Aeth nociceptors, and PGE(2) seems to be more selective to induce this phenomenon than the thermal threshold lowering. In addition, this hyperpathic effect induced by DMSO and capsaicin seems to be indirectly mediated by PGE(2) and C-fibres.
Asunto(s)
Dinoprostona/farmacología , Calor , Hiperalgesia/patología , Fibras Nerviosas Mielínicas/patología , Dolor/fisiopatología , Animales , Antiinflamatorios no Esteroideos/farmacología , Capsaicina/antagonistas & inhibidores , Capsaicina/farmacología , Dimetilsulfóxido/antagonistas & inhibidores , Dimetilsulfóxido/farmacología , Dinoprostona/antagonistas & inhibidores , Indometacina/farmacología , Masculino , Fibras Nerviosas Mielínicas/fisiología , Fibras Nerviosas Amielínicas/efectos de los fármacos , Fibras Nerviosas Amielínicas/patología , Nociceptores/efectos de los fármacos , Dolor/inducido químicamente , Dimensión del Dolor , Umbral del Dolor/efectos de los fármacos , Ratas , Ratas WistarRESUMEN
The duration of noxious stimulus-induced antinociception (NSIA) has been shown to outlast the pain stimulus that elicited it, however, the mechanism that determines the duration of analgesia is unknown. We evaluated the role of spinal excitatory and inhibitory receptors (NMDA, mGluR(5), mu-opioid, GABA(A), and GABA(B)), previously implicated in NSIA initiation, in its maintenance. As in our previous studies, the supraspinal trigeminal jaw-opening reflex (JOR) in the rat was used for nociceptive testing because of its remoteness from the region of drug application, the lumbar spinal cord. NSIA was reversed by antagonists for two inhibitory receptors (GABA(B) and mu-opioid) but not by antagonists for either of the two excitatory receptors (NMDA and mGluR(5)), indicating that NSIA is maintained by ongoing activity at inhibitory synapses in the spinal cord. Furthermore, spinal administration of the GABA(B) agonist baclofen mimicked NSIA in that it could be blocked by prior injection of the mu-opioid receptor antagonist H-D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH(2) (CTAP) in nucleus accumbens. CTAP also blocked baclofen antinociception when administered in the spinal cord. We conclude that analgesia induced by noxious stimulation is maintained by activity in spinal inhibitory receptors.
Asunto(s)
Inhibición Neural/fisiología , Umbral del Dolor/fisiología , Dolor/fisiopatología , Médula Espinal/fisiología , Transmisión Sináptica/fisiología , Analgésicos/antagonistas & inhibidores , Animales , Baclofeno/farmacología , Capsaicina/antagonistas & inhibidores , Interacciones Farmacológicas/fisiología , Agonistas del GABA/farmacología , Antagonistas del GABA/farmacología , Agonistas de Receptores GABA-B , Antagonistas de Receptores de GABA-B , Masculino , Antagonistas de Narcóticos/farmacología , Inhibición Neural/efectos de los fármacos , Dolor/inducido químicamente , Umbral del Dolor/efectos de los fármacos , Péptidos/farmacología , Ratas , Ratas Sprague-Dawley , Receptores de GABA-B/metabolismo , Receptores Opioides mu/antagonistas & inhibidores , Receptores Opioides mu/metabolismo , Fármacos del Sistema Sensorial/antagonistas & inhibidores , Médula Espinal/efectos de los fármacos , Sinapsis/efectos de los fármacos , Sinapsis/fisiología , Transmisión Sináptica/efectos de los fármacosRESUMEN
The present study was designed to investigate further the mechanisms involved in the antinociception caused by bis-selenide in behavioral model of pain in mice. Bis-selenide (5-50 mg/kg), given orally, produced significant inhibition of the antinociceptive behavior induced by intrathecal (i.t.) injection of glutamate (175 nmol/site), kainate (110 pmol/site) and (+/-)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD; 50 nmol/site) and the maximal inhibitions observed were 57+/-5, 46+/-7 and 73+/-3%, respectively. Bis-selenide failed to affect the nociception induced by alpha-amino-3-hydroxy-5-mehtyl-4-isoxazolepropionic acid (AMPA; 135 pmol/site) and N-methyl-d-aspartate (NMDA; 450 pmol/site). This compound also reduced the nociceptive response induced by tumor necrosis factor-alpha (TNF-alpha; 0.1 pg/site), interleukin-1beta (IL-1beta; 1 pg/site), substance P (SP) (135 ng/site, i.t.) and capsaicin (30 ng/site) and the inhibitions observed were 81+/-3%, 88+/-1%, 77+/-3 and 67+/-3, respectively. The oral administration of bis-selenide (25-50 mg/kg) in mice caused a significant increase in the reaction time to thermal stimuli in the hot plate test and the mean ID(50) value (and the 95% confidence limits) was 20.37 (15.00-25.74) mg/kg. The antinociceptive effect caused by bis-selenide (50 mg/kg, p.o.) on the hot plate test in mice was reversed by intrathecal (i.t.) injection of some K(+) channel blockers such as tetraethylammonium (TEA, non-selective voltage-dependent K(+) channel inhibitor) and glibenclamide (ATP-sensitive K(+) channel inhibitor), but not apamin and charybdotoxin (large- and small-conductance Ca(2+)-activated K(+) channel inhibitors, respectively). Together, these results indicate that bis-selenide produces antinociception at spinal sites through the activation of ATP-sensitive and voltage-gated K(+) channels and interaction with kainate and trans-ACDP receptors as well as vanilloid and neuropeptide receptors and pro-inflammatory cytokines.
Asunto(s)
Nociceptores/efectos de los fármacos , Compuestos de Organoselenio/farmacología , Dolor/tratamiento farmacológico , Médula Espinal/efectos de los fármacos , Administración Oral , Analgésicos/química , Analgésicos/farmacología , Animales , Capsaicina/antagonistas & inhibidores , Citocinas/antagonistas & inhibidores , Relación Dosis-Respuesta a Droga , Agonistas de Aminoácidos Excitadores/farmacología , Femenino , Inyecciones Espinales , Ratones , Estructura Molecular , Nociceptores/metabolismo , Dolor/inducido químicamente , Dolor/fisiopatología , Umbral del Dolor/efectos de los fármacos , Umbral del Dolor/fisiología , Bloqueadores de los Canales de Potasio/farmacología , Canales de Potasio/efectos de los fármacos , Canales de Potasio/metabolismo , Tiempo de Reacción/efectos de los fármacos , Tiempo de Reacción/fisiología , Receptores de Ácido Kaínico/efectos de los fármacos , Receptores de Ácido Kaínico/metabolismo , Receptores de Glutamato Metabotrópico/efectos de los fármacos , Receptores de Glutamato Metabotrópico/metabolismo , Médula Espinal/metabolismo , Médula Espinal/fisiopatologíaRESUMEN
The anti-inflammatory pentacyclic triterpene, oleanolic acid (OA) was examined on acute nociception induced by intraplantar injection of capsaicin in mice. OA administered orally to mice at 10, 30 and 100 mgkg(-1), significantly attenuated the paw-licking response to capsaicin (1.6 microg/paw) by 53%, 68.5% and 36.6%, respectively. Ruthenium red (3 mgkg(-1), s.c.), a non-competitive vanilloid receptor (V1, TRPV1)-antagonist also suppressed the capsaicin nociception by 38.6%. The maximal antinociception produced by 30 mgkg(-1) OA was significantly blocked in animals pre-treated with naloxone (2 mgkg(-1), i.p.), the opioid antagonist; l-arginine (600 mgkg(-1), i.p.), the substrate for nitric oxide synthase; or glibenclamide (2 mgkg(-1), i.p.), the K(ATP)-channel blocker, but was unaffected by yohimbine (2 mgkg(-1), i.p.), an alpha(2)-adrenoceptor antagonist. In open-field and rota-rod tests that detect motor deficits, mice received 30 mgkg(-1) OA did not manifest any effect per se, indicating that the observed antinociception is not a consequence of motor abnormality. These data suggest that OA inhibits capsaicin-evoked acute nociception due to mechanisms possibly involving endogenous opioids, nitric oxide, and K(ATP)-channel opening.
Asunto(s)
Capsaicina/antagonistas & inhibidores , Ácido Oleanólico/farmacología , Dolor/inducido químicamente , Dolor/tratamiento farmacológico , Antagonistas Adrenérgicos alfa/farmacología , Analgésicos Opioides/farmacología , Animales , Capsaicina/farmacología , Colorantes , Inhibidores Enzimáticos/farmacología , Gliburida/farmacología , Hipoglucemiantes/farmacología , Canales KATP , Lamiaceae/química , Masculino , Ratones , Morfina/farmacología , Actividad Motora/efectos de los fármacos , NG-Nitroarginina Metil Éster/farmacología , Naloxona/farmacología , Antagonistas de Narcóticos/farmacología , Dimensión del Dolor/efectos de los fármacos , Equilibrio Postural/efectos de los fármacos , Canales de Potasio de Rectificación Interna/efectos de los fármacos , Canales de Potasio de Rectificación Interna/metabolismo , Rojo de Rutenio , Yohimbina/farmacologíaRESUMEN
Curatella americana L. (Dilleneaceae) popularly known as 'cajueiro-bravo' and 'sambaiba' is used in Brazilian folk medicine for the treatment of inflammation and ulcer. The anti-inflammatory and analgesic tests were conducted with the hydroalcoholic extract (HAE) of the bark of the plant. The HAE inhibited mouse ear oedema induced by o-tetradecanoyl phorbol acetate (TPA) and by capsaicin. While the ID50 values obtained for the HAE against these two irritants were 40.8 +/- 1.7 and 30 +/- 1.2 mg/kg i.p. (mean +/- S.E.M., n = 6), respectively, the corresponding value for carrageenan induced paw oedema (3 h) was 21.8 +/- 2.1 mg/kg, i.p., n = 6. In the established adjuvant-induced arthritis model, the HAE significantly inhibited the oedema in daily doses of 50 mg/kg, i.p. (n = 10). The HAE also inhibited acetic acid-induced writhing (ID50 23.2 +/- 0.8 mg/kg, i.p., n = 6) and the formalin-induced late phase paw licking response (ID50 11.9 +/- 1.2 mg/kg, i.p., n = 10) in the mice. However, the HAE was inactive in the formalin-induced initial paw licking response in mice or heat induced tail flick response in rats. The HAE has shown both anti-inflammatory and peripheral analgesic activities when administrated in the mouse by the intraperitoneal route in doses which are at least 12 times lower than its LD50 dose of 647 mg/kg, i.p.
Asunto(s)
Analgésicos , Antiinflamatorios/uso terapéutico , Artritis/tratamiento farmacológico , Edema/prevención & control , Medicina Tradicional , Extractos Vegetales/uso terapéutico , Ácido Acético/antagonistas & inhibidores , Ácido Acético/toxicidad , Animales , Artritis/inducido químicamente , Brasil , Capsaicina/antagonistas & inhibidores , Capsaicina/toxicidad , Carcinógenos/antagonistas & inhibidores , Carcinógenos/toxicidad , Relación Dosis-Respuesta a Droga , Edema/inducido químicamente , Indicadores y Reactivos/toxicidad , Dosificación Letal Mediana , Masculino , Ratones , Ratas , Ratas Wistar , Conducta Estereotipada/efectos de los fármacos , Acetato de Tetradecanoilforbol/antagonistas & inhibidores , Acetato de Tetradecanoilforbol/toxicidadRESUMEN
Superfusion of capsaicin onto the serosal surface of jejunum of Nippostrongylus brasiliensis-sensitized rats induces a short-lasting (1-3 min), dose-dependent (2 to 20 micrograms) decrease in blood pressure which ranges from -5.3 +/- 1.4% to -22.6 +/- 2.2%. The hypotension evoked by capsaicin was more marked in sensitized rats than in unsensitized animals, which responded only to the highest dose (20 mg) of capsaicin tested. The hypotensive effects of capsaicin were not affected by intravenous injections of mepyramine (10 mg/kg), a histamine receptor antagonist, or by the cyclooxygenase inhibitor indomethacin (10 mg/kg). However, an intravenous injection of a platelet-activating factor (PAF) antagonist, BN 52021 (20 mg/kg), or an intraperitoneal injection of guanethidine (8 mg/kg) 18 h prior to experimentation, to functionally impair the sympathetic nerves, abolished the capsaicin-induced drop in blood pressure. Treatment of neonatal rats with capsaicin reduced by 75% the hypotensive effects of capsaicin, whereas the capsaicin antagonist, ruthenium red, reduced non-significantly the hypotensive action of capsaicin. It is concluded that the activation of jejunal sensory nerves in N. brasiliensis-sensitized rats by capsaicin induced a reflex hypotension that is dependent upon PAF release from mast cells and functional sympathetic nerves. In addition, the afferent function of the sensory nerves are not totally blocked by ruthenium red as capsaicin elicits the reflex hypotension in the presence of this blocker of sensory nerve efferent function.