RESUMEN
Objectives: Many studies indicate the involvement of transient receptor potential (TRP) channels in the development of heart hypertrophy. However, the data is often conflicted and has originated in animal models. Here, we provide systematic analysis of TRP channels expression in human failing myocardium. Methods and results: Left-ventricular tissue samples were isolated from explanted hearts of NYHA III-IV patients undergoing heart transplants (n = 43). Quantitative real-time PCR was performed to assess the mRNA levels of TRPC, TRPM and TRPV channels. Analysis of functional, clinical and biochemical data was used to confirm an end-stage heart failure diagnosis. Compared to myocardium samples from healthy donor hearts (n = 5), we detected a distinct increase in the expression of TRPC1, TRPC5, TRPM4 and TRPM7, and decreased expression of TRPC4 and TRPV2. These changes were not dependent on gender, clinical or biochemical parameters, nor functional parameters of the heart. We detected, however, a significant correlation of TRPC1 and MEF2c expression. Conclusions: The end-stage heart failure displays distinct expressional changes of TRP channels. Our findings provide a systematic description of TRP channel expression in human heart failure. The results highlight the complex interplay between TRP channels and the need for deeper analysis of early stages of hypertrophy and heart failure development.
Asunto(s)
Insuficiencia Cardíaca/fisiopatología , Trasplante de Corazón/efectos adversos , Canales de Potencial de Receptor Transitorio/análisis , Análisis de Varianza , Femenino , Insuficiencia Cardíaca/sangre , Insuficiencia Cardíaca/complicaciones , Trasplante de Corazón/métodos , Humanos , Masculino , Persona de Mediana Edad , Proteínas Serina-Treonina Quinasas/análisis , Proteínas Serina-Treonina Quinasas/sangre , Reacción en Cadena en Tiempo Real de la Polimerasa/métodos , Estadísticas no Paramétricas , Canales Catiónicos TRPC/análisis , Canales Catiónicos TRPC/sangre , Canales Catiónicos TRPM/análisis , Canales Catiónicos TRPM/sangre , Canales de Potencial de Receptor Transitorio/sangre , Canales de Potencial de Receptor Transitorio/farmacologíaRESUMEN
A critical challenge for chemotherapy is the development of chemoresistance in breast cancer. However, the underlying mechanisms and validated predictors remain unclear. Extracellular vesicles (EVs) have gained attention as potential means for cancer cells to share intracellular contents. In adriamycin-resistant human breast cancer cells (MCF-7/ADM), we analyzed the role of transient receptor potential channel 5 (TrpC5) in EV formation and transfer as well as the diagnostic implications. Up-regulated TrpC5, accumulated in EVs, is responsible for EV formation and trapping of adriamycin (ADM) in EVs. EV-mediated intercellular transfer of TrpC5 allowed recipient cells to acquire TrpC5, consequently stimulating multidrug efflux transporter P-glycoprotein production through a Ca(2+)- and activated T-cells isoform c3-mediated mechanism and thus, conferring chemoresistance on nonresistant cells. TrpC5-containing circulating EVs were detected in nude mice bearing MCF-7/ADM tumor xenografts, and the level was lower after TrpC5-siRNA treatment. In breast cancer patients who underwent chemotherapy, TrpC5 expression in the tumor was significantly higher in patients with progressive or stable disease than in patients with a partial or complete response. TrpC5-containing circulating EVs were found in peripheral blood from patients who underwent chemotherapy but not patients without chemotherapy. Taken together, we found that TrpC5-containing circulating EVs may transfer chemoresistance property to nonchemoresistant recipient cells. It may be worthwhile to further explore the potential of using TrpC5-containing EVs as a diagnostic biomarker for chemoresistant breast cancer.
Asunto(s)
Neoplasias de la Mama/metabolismo , Neoplasias de la Mama/patología , Vesículas Citoplasmáticas/metabolismo , Resistencia a Antineoplásicos , Canales Catiónicos TRPC/metabolismo , Animales , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/ultraestructura , Línea Celular Tumoral , Vesículas Citoplasmáticas/efectos de los fármacos , Vesículas Citoplasmáticas/ultraestructura , Doxorrubicina/farmacología , Doxorrubicina/uso terapéutico , Resistencia a Antineoplásicos/efectos de los fármacos , Femenino , Humanos , Ratones , Ratones Desnudos , Canales Catiónicos TRPC/sangre , Resultado del Tratamiento , Ensayos Antitumor por Modelo de XenoinjertoRESUMEN
We determined whether store-operated channels (SOC) are involved in neonatal pulmonary artery function under conditions of acute and chronic hypoxia, using newborn sheep gestated and born either at high altitude (HA, 3,600 m) or low altitude (LA, 520 m). Cardiopulmonary variables were recorded in vivo, with and without SOC blockade by 2-aminoethyldiphenylborinate (2-APB), during basal or acute hypoxic conditions. 2-APB did not have effects on basal mean pulmonary arterial pressure (mPAP), cardiac output, systemic arterial blood pressure, or systemic vascular resistance in both groups of neonates. During acute hypoxia 2-APB reduced mPAP and pulmonary vascular resistance in LA and HA, but this reduction was greater in HA. In addition, isolated pulmonary arteries mounted in a wire myograph were assessed for vascular reactivity. HA arteries showed a greater relaxation and sensitivity to SOC blockers than LA arteries. The pulmonary expression of two SOC-forming subunits, TRPC4 and STIM1, was upregulated in HA. Taken together, our results show that SOC contribute to hypoxic pulmonary vasoconstriction in newborn sheep and that SOC are upregulated by chronic hypoxia. Therefore, SOC may contribute to the development of neonatal pulmonary hypertension. We propose SOC channels could be potential targets to treat neonatal pulmonary hypertension.
Asunto(s)
Altitud , Canales Iónicos/fisiología , Circulación Pulmonar/fisiología , Oveja Doméstica/fisiología , Mal de Altura/sangre , Mal de Altura/complicaciones , Mal de Altura/genética , Mal de Altura/fisiopatología , Animales , Animales Recién Nacidos , Compuestos de Boro/farmacología , Modelos Animales de Enfermedad , Hemodinámica/efectos de los fármacos , Hemodinámica/fisiología , Humanos , Hipoxia/sangre , Hipoxia/complicaciones , Hipoxia/genética , Hipoxia/fisiopatología , Recién Nacido , Canales Iónicos/sangre , Canales Iónicos/genética , Síndrome de Circulación Fetal Persistente/sangre , Síndrome de Circulación Fetal Persistente/etiología , Síndrome de Circulación Fetal Persistente/fisiopatología , Arteria Pulmonar/fisiopatología , Circulación Pulmonar/efectos de los fármacos , Oveja Doméstica/sangre , Oveja Doméstica/genética , Canales Catiónicos TRPC/sangre , Canales Catiónicos TRPC/fisiología , Vasoconstricción/fisiologíaRESUMEN
Ca(2+)influx might occur through K(+)-dependent Na(+)/Ca(2+) exchanger operating in reverse mode (rNCKX). In a cellular model different from platelets, an interaction between canonical transient receptor potential cation (TRPC) channels and NCX has been found. The aim of this study was to verify whether the TRPC/NCKX interaction operates in human platelets. Our results showed that the diacylglycerol (DAG) analogue, 1-oleoyl-2-acetyl-sn-glycerol (OAG) induced rNCKX-mediated Ca(2+) influx through TRPC-mediated Na(+) influx. DAG-induced activation of TRPC/NCKX occurs independently of protein kinase C (PKC) activation, as PKC inhibitor did not modify OAG-mediated Ca(2+) influx. Moreover, as both rNCKX and TRPC inhibitors reduced OAG-induced platelet aggregation which, conversely, was increased by flufenamic acid, known to develop TRPC activity, it could be suggested that the TRPC/NCKX interaction has a role in OAG-dependent platelet aggregation.
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Plaquetas/efectos de los fármacos , Canales de Calcio/sangre , Calcio/sangre , Diglicéridos/farmacología , Proteína Quinasa C/sangre , Canales Catiónicos TRPC/sangre , Plaquetas/citología , Plaquetas/metabolismo , Activación Enzimática , Humanos , Agregación Plaquetaria/efectos de los fármacos , Agregación Plaquetaria/fisiología , Transducción de SeñalRESUMEN
Increased transient receptor potential canonical type 3 (TRPC3) channels have been observed in patients with essential hypertension. In the present study we tested the hypothesis that increased monocyte migration is associated with increased TRPC3 expression. Monocyte migration assay was performed in a microchemotaxis chamber using chemoattractants formylated peptide Met-Leu-Phe (fMLP) and tumor necrosis factor-α (TNF-α). Proteins were identified by immunoblotting and quantitative in-cell Western assay. The effects of TRP channel-inhibitor 2-aminoethoxydiphenylborane (2-APB) and small interfering RNA knockdown of TRPC3 were investigated. We observed an increased fMLP-induced migration of monocytes from hypertensive patients compared with normotensive control subjects (246 ± 14% vs 151 ± 10%). The TNF-α-induced migration of monocytes in patients with essential hypertension was also significantly increased compared to normotensive control subjects (221 ± 20% vs 138 ± 18%). In the presence of 2-APB or after siRNA knockdown of TRPC3 the fMLP-induced monocyte migration was significantly blocked. The fMLP-induced changes of cytosolic calcium were significantly increased in monocytes from hypertensive patients compared to normotensive control subjects. The fMLP-induced monocyte migration was significantly reduced in the presence of inhibitors of tyrosine kinase and phosphoinositide 3-kinase. We conclude that increased monocyte migration in patients with essential hypertension is associated with increased TRPC3 channels.
Asunto(s)
Hipertensión/sangre , Monocitos/fisiología , Canales Catiónicos TRPC/sangre , Anciano , Secuencia de Bases , Compuestos de Boro/farmacología , Calcio/sangre , Estudios de Casos y Controles , Movimiento Celular , Citosol/metabolismo , Femenino , Técnicas de Silenciamiento del Gen , Humanos , Técnicas In Vitro , Sistema de Señalización de MAP Quinasas , Masculino , Persona de Mediana Edad , Monocitos/clasificación , Monocitos/efectos de los fármacos , N-Formilmetionina Leucil-Fenilalanina/farmacología , Fosfatidilinositol 3-Quinasas/sangre , Proteínas Tirosina Quinasas/sangre , Proteínas Proto-Oncogénicas c-akt/sangre , ARN Interferente Pequeño/genética , Canales Catiónicos TRPC/agonistas , Canales Catiónicos TRPC/antagonistas & inhibidores , Canales Catiónicos TRPC/genéticaRESUMEN
PURPOSE: Our aim is to verify the association of three Single nucleotide polymorphisms (SNPs) (-218A/G, -254C/G, -361A/T) in the promoter of TRPC6 in 168 sporadic cases with infantile hypertrophic pyloric stenosis (IHPS) and 164 controls in Chinese people. METHODS: All participants were genotyped using polymerase chain reaction and direct sequencing. And the χ(2) value was calculated. A value of P less than 0.05 was considered statistically significant. We also got the P value of Hardy-Weinberg equilibrium test, and the value of P greater than 0.05 was assumed to be at Hardy-Weinberg equilibrium in this population. RESULTS: The results tell us that there are no significant differences in the allele and genotype frequencies of all these three SNPs between the case and the control groups (P > 0.05). CONCLUSION: These three TRPC6 SNPs have no association with the IHPS in Chinese people. However, we cannot deny that TRPC6 would be a susceptible gene with IHPS in Chinese people. May be other SNPs in TRPC6 would have some association with the IHPS in Chinese people. But in this study our results may be due to the fact that these SNPs are not the functional SNPs for the development of IHPS in Chinese people.
Asunto(s)
ADN/genética , Predisposición Genética a la Enfermedad , Polimorfismo de Nucleótido Simple , Regiones Promotoras Genéticas , Estenosis Hipertrófica del Piloro/genética , Canales Catiónicos TRPC/genética , China/epidemiología , Femenino , Frecuencia de los Genes , Genotipo , Humanos , Incidencia , Lactante , Recién Nacido , Masculino , Reacción en Cadena de la Polimerasa , Estenosis Hipertrófica del Piloro/sangre , Estenosis Hipertrófica del Piloro/epidemiología , Canales Catiónicos TRPC/sangre , Canal Catiónico TRPC6RESUMEN
Ca(2+) entry, particularly store-operated Ca(2+) entry (SOCE), has been reported to be crucial for a variety of cellular functions. SOCE is a mechanism regulated by the Ca(2+) content of the stores, where the intraluminal Ca(2+) sensor STromal Interaction Molecule 1 (STIM1) has been reported to communicate the filling state of the intracellular Ca(2+) stores to the store-operated Ca(2+)-permeable channels in the plasma membrane, likely involving Orai1 and TRPC proteins, such as TRPC1. Here we have investigated the role of Orai1, STIM1 and TRPC1 in platelet aggregation, an event that occurs during the process of thrombosis and hemostasis. Electrotransjection of cells with anti-STIM1 (25-139) antibody, directed towards the Ca(2+)-binding motif, significantly reduced thrombin-induced aggregation and prevented ADP-evoked response. Extracellular application of the anti-STIM1 antibody, in order to block the function of plasma membrane-located STIM1, reduced thrombin- and ADP-stimulated platelet aggregation to a lesser extent. Introduction of an anti-Orai1 (288-301) antibody, which binds the STIM1-binding site located in the Orai1 C-terminus, or extracellular application of anti-hTRPC1 (557-571) antibody to impair hTRPC1 channel function, significantly reduced thrombin- and ADP-induced platelet aggregation. These findings suggest a role of STIM1, Orai1 and hTRPC1 in thrombin- and ADP-induced platelet aggregation probably through the regulation of Ca(2+) entry, which might become targets for the development of therapeutic strategies to treat platelet hyperactivity and thrombosis disorders.
Asunto(s)
Adenosina Difosfato/farmacología , Canales de Calcio/sangre , Proteínas de la Membrana/sangre , Proteínas de Neoplasias/sangre , Agregación Plaquetaria/efectos de los fármacos , Agregación Plaquetaria/fisiología , Canales Catiónicos TRPC/sangre , Trombina/farmacología , Animales , Anticuerpos/administración & dosificación , Plaquetas/efectos de los fármacos , Plaquetas/fisiología , Calcio/farmacología , Canales de Calcio/inmunología , Señalización del Calcio , Humanos , Técnicas In Vitro , Proteínas de la Membrana/antagonistas & inhibidores , Proteínas de la Membrana/inmunología , Proteínas de Neoplasias/antagonistas & inhibidores , Proteínas de Neoplasias/inmunología , Proteína ORAI1 , Molécula de Interacción Estromal 1 , Canales Catiónicos TRPC/antagonistas & inhibidores , Canales Catiónicos TRPC/inmunologíaRESUMEN
Upon stimulation, activation of NADPH oxidase complexes in neutrophils produces a burst of superoxide anions contributing to oxidative stress and the development of inflammatory process. Store-operated calcium entry (SOCE), whereby the depletion of intracellular stores induces extracellular calcium influx, is known to be a crucial element of NADPH oxidase regulation. However, the mechanistic basis mediating SOCE is still only partially understood, as is the signal-coupling pathway leading to modulation of store-operated channels. This review emphasizes the role of calcium influx in the control of the NADPH oxidase and summarizes the current knowledge of pathways mediating this extracellular calcium entry in neutrophils. Such investigations into the cross-talk between NADPH oxidase and calcium might allow the identification of novel pharmacological targets with clinical use, particularly in inflammatory diseases.
Asunto(s)
Calcio/sangre , NADPH Oxidasas/sangre , Neutrófilos/fisiología , Superóxidos/sangre , Citosol/enzimología , Humanos , Neutrófilos/enzimología , Fagocitosis , Fosfolipasas A2/sangre , Transducción de Señal , Canales Catiónicos TRPC/sangreRESUMEN
Changes in [Ca(2+)](i) are a central step in platelet activation. In nonexcitable cells, receptor-mediated depletion of intracellular Ca(2+) stores triggers Ca(2+) entry through store-operated calcium (SOC) channels. Stromal interaction molecule 1 (STIM1) has been identified as an endoplasmic reticulum (ER)-resident Ca(2+) sensor that regulates store-operated calcium entry (SOCE), but the identity of the SOC channel in platelets has been controversially debated. Some investigators proposed transient receptor potential (TRP) C1 to fulfil this function based on the observation that antibodies against the channel impaired SOCE in platelets. However, others could not detect TRPC1 in the plasma membrane of platelets and raised doubts about the specificity of the inhibiting anti-TRPC1 antibodies. To address the role of TRPC1 in SOCE in platelets, we analyzed mice lacking TRPC1. Platelets from these mice display fully intact SOCE and also otherwise unaltered calcium homeostasis compared to wild-type. Furthermore, platelet function in vitro and in vivo is not altered in the absence of TRPC1. Finally, studies on human platelets revealed that the presumably inhibitory anti-TRPC1 antibodies have no specific effect on SOCE and fail to bind to the protein. Together, these results provide evidence that SOCE in platelets is mediated by channels other than TRPC1.
Asunto(s)
Plaquetas/metabolismo , Señalización del Calcio , Calcio/sangre , Canales Catiónicos TRPC/sangre , Animales , Coagulación Sanguínea , Cloruros , Modelos Animales de Enfermedad , Compuestos Férricos , Humanos , Proteínas de la Membrana/sangre , Ratones , Ratones Noqueados , Proteínas de Neoplasias/sangre , Activación Plaquetaria , Molécula de Interacción Estromal 1 , Canales Catiónicos TRPC/deficiencia , Canales Catiónicos TRPC/genética , Trombosis/sangre , Trombosis/inducido químicamente , Factores de TiempoRESUMEN
BACKGROUND: Transient receptor potential canonical type 6 (TRPC6) channels mediating 1-oleoyl-2-acetyl-sn-glycerol (OAG)-induced calcium entry have been identified on human platelets. In the present study we tested the hypothesis that hyperglycemia increases the expression of TRPC6 channels. METHODS AND RESULTS: Platelets from healthy control subjects and patients with type 2 diabetes mellitus were incubated with glucose and calcium influx was measured using the fluorescent dye technique. TRPC channel protein expression was investigated using immunofluorescence and fluorescence microscopy of single platelets. Administration of 25 mmol/L glucose significantly enhanced the OAG-induced calcium influx, which was attenuated by inhibitors of the phosphatidylinositol 3-kinase, wortmannin or LY294002. The glucose-enhanced and OAG-induced calcium influx was concentration- and time-dependent. Glucose significantly increased the TRPC6 protein expression in platelets to 131+/-12% (n=33; P<0.05), whereas the expression of TRPC1, TRPC3, TRPC4, or TRPC5 were unchanged. The glucose-induced TRPC6 expression was significantly attenuated in the presence of wortmannin or LY294002. Platelets from patients with type 2 diabetes mellitus showed increased TRPC6 expression compared to nondiabetic individuals (P<0.05). CONCLUSIONS: The study indicates that high glucose increases TRPC6 channel protein expression on the platelet surface which is mediated by a phosphatidylinositol 3-kinase-dependent pathway.
Asunto(s)
Plaquetas/metabolismo , Señalización del Calcio/fisiología , Hiperglucemia/sangre , Fosfatidilinositol 3-Quinasas/sangre , Canales Catiónicos TRPC/sangre , Anciano , Androstadienos/farmacología , Glucemia/metabolismo , Plaquetas/efectos de los fármacos , Señalización del Calcio/efectos de los fármacos , Estudios de Casos y Controles , Cromonas/farmacología , Diabetes Mellitus Tipo 2/sangre , Angiopatías Diabéticas/sangre , Angiopatías Diabéticas/etiología , Diglicéridos/farmacología , Inhibidores Enzimáticos/farmacología , Técnica del Anticuerpo Fluorescente , Glucosa/farmacología , Humanos , Técnicas In Vitro , Persona de Mediana Edad , Modelos Biológicos , Morfolinas/farmacología , Selectina-P/sangre , Inhibidores de las Quinasa Fosfoinosítidos-3 , Activación Plaquetaria/efectos de los fármacos , Canal Catiónico TRPC6 , WortmaninaRESUMEN
Focal and segmental glomerulosclerosis (FSGS) is a pathologic entity that is a common and increasing cause of end-stage renal disease. Typical manifestations include proteinuria, hypertension, worsening renal insufficiency, and, frequently, renal failure. The etiology, however, remains unknown in a majority of patients. There is an estimated recurrence rate of 30% to 40% in renal transplant patients, suggesting that the pathogenesis is not solely a result of intrinsic kidney disease. Although some of its characteristics have been reported, the precise identification of a circulating factor associated with FSGS has not been made. Remarkable progress has been made in recent years regarding biologic mechanisms surrounding FSGS and proteinuria. Insight into the pathogenesis of FSGS has been gained through the study of hereditary forms of FSGS and nephrotic syndromes. Mutations in cytoskeletal proteins that affect podocyte structure have been the target until recently. Here we review the current understanding of this glomerular disease and areas for future concentration.