RESUMEN
Objetivou-se com estudo determinar a ocorrência da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco, Brasil. Foram coletadas 133 amostras biológicas (muco cervicovaginal e raspado prepucial) de animais, procedentes de oito propriedades, de diferentes regiões do estado. O material biológico coletado foi transferido para solução salina tamponada (PBS) e, posteriormente, inoculado em meios de transporte específicos, Lander para diagnóstico de C. fetus subsp. venerealis e Diamond para T. foetus. Para o diagnóstico das infecções por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus, as amostras foram submetidas à reação em cadeia da polimerase (PCR) e cultivadas em meio ágar Columbia acrescido de antibiótico e Diamond, respectivamente. Para pesquisa de C. fetus subsp. venerealis, observou-se uma ocorrência de 1,8% (2/113) de animais positivos no exame microbiológico com confirmação pela PCR. Em relação à procedência, observou-se que 100% das amostras positivas pertenciam a dois machos do mesmo rebanho. Nenhum animal foi positivo na pesquisa de T. foetus. Este é o primeiro registro da infecção por C. fetus subsp. venerealis em búfalos no Brasil. Apesar da baixa ocorrência, recomenda-se adoção de medidas de controle, com o intuito de se evitar a disseminação do agente para outros rebanhos.(AU)
The objective this study was to determine the occurrence of infection with Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in buffaloes in the State of Pernambuco, Brazil. Biological samples were collected (cervico vaginal mucus and shaved prepucial) of 113 animals, coming from 8 properties in different regions of the state. The biological material collected was transferred into phosphate buffered saline (PBS) and inoculated in the specific transport, Lander for diagnosis of C. fetus subsp. venerealis and Diamond for T. fetus subsequently. For the diagnosis of infection by Campylobacter fetus subsp. venrealis and Tritrichomonas foetus the samples were submitted to Polymerase Chain Reaction (PCR) grown in Columbia agar plus antibiotics and Diamond, respectively. There was an occurrence of 1.8% (2/113) of positive animals in the microbiological examination with confirmation by PCR, for C. fetus subsp. venerealis. We observed that 100% of positive samples were from two (2) males from the same herd. No animals were positive for T. foetus. This is the first report of infection with C. fetus subsp. venerealis in buffaloes in Brazil. Despite rare occurrence, control measures are recommended in order to prevent the spread of the agent to other herds.(AU)
Asunto(s)
Animales , Búfalos/microbiología , Tritrichomonas foetus/patogenicidad , Campylobacter fetus/patogenicidad , Medidas de Ocurrencia de EnfermedadesRESUMEN
Objetivou-se com estudo determinar a ocorrência da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco, Brasil. Foram coletadas 133 amostras biológicas (muco cervicovaginal e raspado prepucial) de animais, procedentes de oito propriedades, de diferentes regiões do estado. O material biológico coletado foi transferido para solução salina tamponada (PBS) e, posteriormente, inoculado em meios de transporte específicos, Lander para diagnóstico de C. fetus subsp. venerealis e Diamond para T. foetus. Para o diagnóstico das infecções por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus, as amostras foram submetidas à reação em cadeia da polimerase (PCR) e cultivadas em meio ágar Columbia acrescido de antibiótico e Diamond, respectivamente. Para pesquisa de C. fetus subsp. venerealis, observou-se uma ocorrência de 1,8% (2/113) de animais positivos no exame microbiológico com confirmação pela PCR. Em relação à procedência, observou-se que 100% das amostras positivas pertenciam a dois machos do mesmo rebanho. Nenhum animal foi positivo na pesquisa de T. foetus. Este é o primeiro registro da infecção por C. fetus subsp. venerealis em búfalos no Brasil. Apesar da baixa ocorrência, recomenda-se adoção de medidas de controle, com o intuito de se evitar a disseminação do agente para outros rebanhos.(AU)
The objective this study was to determine the occurrence of infection with Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in buffaloes in the State of Pernambuco, Brazil. Biological samples were collected (cervico vaginal mucus and shaved prepucial) of 113 animals, coming from 8 properties in different regions of the state. The biological material collected was transferred into phosphate buffered saline (PBS) and inoculated in the specific transport, Lander for diagnosis of C. fetus subsp. venerealis and Diamond for T. fetus subsequently. For the diagnosis of infection by Campylobacter fetus subsp. venrealis and Tritrichomonas foetus the samples were submitted to Polymerase Chain Reaction (PCR) grown in Columbia agar plus antibiotics and Diamond, respectively. There was an occurrence of 1.8% (2/113) of positive animals in the microbiological examination with confirmation by PCR, for C. fetus subsp. venerealis. We observed that 100% of positive samples were from two (2) males from the same herd. No animals were positive for T. foetus. This is the first report of infection with C. fetus subsp. venerealis in buffaloes in Brazil. Despite rare occurrence, control measures are recommended in order to prevent the spread of the agent to other herds.(AU)
Asunto(s)
Animales , Búfalos/microbiología , Campylobacter fetus/patogenicidad , Medidas de Ocurrencia de Enfermedades , Tritrichomonas foetus/patogenicidadRESUMEN
Campylobacter fetus is a venereal pathogen of cattle and sheep, and an opportunistic human pathogen. It is often assumed that C. fetus infection occurs in humans as a zoonosis through food chain transmission. Here we show that mammalian C. fetus consists of distinct evolutionary lineages, primarily associated with either human or bovine hosts. We use whole-genome phylogenetics on 182 strains from 17 countries to provide evidence that C. fetus may have originated in humans around 10,500 years ago and may have "jumped" into cattle during the livestock domestication period. We detect C. fetus genomes in 8% of healthy human fecal metagenomes, where the human-associated lineages are the dominant type (78%). Thus, our work suggests that C. fetus is an unappreciated human intestinal pathobiont likely spread by human to human transmission. This genome-based evolutionary framework will facilitate C. fetus epidemiology research and the development of improved molecular diagnostics and prevention schemes for this neglected pathogen.
Asunto(s)
Infecciones por Campylobacter/transmisión , Campylobacter fetus/genética , Campylobacter fetus/patogenicidad , Microbioma Gastrointestinal , Animales , Infecciones por Campylobacter/veterinaria , Bovinos , Enfermedades de los Bovinos/microbiología , Enfermedades de los Bovinos/transmisión , Heces/microbiología , Interacciones Huésped-Patógeno , Humanos , Masculino , FilogeniaRESUMEN
Bovine genital campylobacteriosis is a reproductive disease that affects cattle production. It is caused by Campylobacter fetus subspecies, C. fetus fetus (Cff) and C. fetus venerealis (Cfv). The aim of this study was to identify the presence of C. fetus in genital fluids by bacteriological culture and direct immunofluorescence (DIF) and to compare the results. Two groups of 6 heifers and 5 bulls, one infected with Cff (Cff group) and the other with Cfv (Cfv group) were formed. Two heifers and 2 bulls, all of them uninfected, made up the control group. Samples of cervicovaginal mucus and preputial fluid were processed by culture and DIF. In the Cff group, 100% of the heifers and 80% of the bulls were infected, while in the Cfv group, 50% of the heifers and 60% of the bulls were infected. The degree of agreement (Kappa values) from benchmarking diagnostic techniques were 0.57 for heifers in the Cff group and 0.52 for heifers in the Cfv group, whereas the values for bulls were 0.17 and 0.27, respectively. Heifers yielded more positive results in the DIF assay than in the culture, exhibiting 5.6% increase in the Cff group and 7.4% in the Cfv group. The lowest percentage of positive results for DIF in bulls, 40% less for the Cff group and 5.2% for the Cfv group, could be due to improper sampling. Kappa values showed moderate agreement for the heifers and low for the bulls.
Asunto(s)
Técnicas Bacteriológicas , Líquidos Corporales/microbiología , Infecciones por Campylobacter/veterinaria , Campylobacter fetus/aislamiento & purificación , Enfermedades de los Bovinos/microbiología , Enfermedades de los Genitales Femeninos/virología , Enfermedades de los Genitales Masculinos/veterinaria , Animales , Antígenos Bacterianos/análisis , Infecciones por Campylobacter/microbiología , Campylobacter fetus/clasificación , Campylobacter fetus/crecimiento & desarrollo , Campylobacter fetus/patogenicidad , Bovinos , Cuello del Útero/microbiología , Femenino , Técnica del Anticuerpo Fluorescente Directa , Prepucio/microbiología , Enfermedades de los Genitales Femeninos/microbiología , Enfermedades de los Genitales Masculinos/microbiología , Masculino , Embarazo , Complicaciones Infecciosas del Embarazo/microbiología , Complicaciones Infecciosas del Embarazo/veterinaria , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Especificidad de la Especie , Vagina/microbiología , VirulenciaRESUMEN
The genus Campylobacter contains pathogens causing a wide range of diseases, targeting both humans and animals. Among them, the Campylobacter fetus subspecies fetus and venerealis deserve special attention, as they are the etiological agents of human bacterial gastroenteritis and bovine genital campylobacteriosis, respectively. We compare the whole genomes of both subspecies to get insights into genomic architecture, phylogenetic relationships, genome conservation and core virulence factors. Pan-genomic approach was applied to identify the core- and pan-genome for both C. fetus subspecies and members of the genus. The C. fetus subspecies conserved (76%) proteome were then analyzed for their subcellular localization and protein functions in biological processes. Furthermore, with pathogenomic strategies, unique candidate regions in the genomes and several potential core-virulence factors were identified. The potential candidate factors identified for attenuation and/or subunit vaccine development against C. fetus subspecies contain: nucleoside diphosphate kinase (Ndk), type IV secretion systems (T4SS), outer membrane proteins (OMP), substrate binding proteins CjaA and CjaC, surface array proteins, sap gene, and cytolethal distending toxin (CDT). Significantly, many of those genes were found in genomic regions with signals of horizontal gene transfer and, therefore, predicted as putative pathogenicity islands. We found CRISPR loci and dam genes in an island specific for C. fetus subsp. fetus, and T4SS and sap genes in an island specific for C. fetus subsp. venerealis. The genomic variations and potential core and unique virulence factors characterized in this study would lead to better insight into the species virulence and to more efficient use of the candidates for antibiotic, drug and vaccine development.
Asunto(s)
Campylobacter fetus/clasificación , Campylobacter fetus/genética , Genes Bacterianos , Genoma Bacteriano , Islas Genómicas/genética , Factores de Virulencia/genética , Virulencia/genética , Animales , Infecciones por Campylobacter/microbiología , Campylobacter fetus/patogenicidad , Bovinos , ADN Bacteriano/genética , Humanos , Filogenia , Análisis de Secuencia de ADN , Especificidad de la EspecieRESUMEN
A polirradiculoneurite desmielinizante inflamatoria aguda(Sindrome de Guillain-Barre) pode apresentar-se de modo severo em criancas. Os criterios clinicos do exame do liquido cefalorraqueano eletromiograficos, importantes para o diagnostico, sao revisados. E apresentado o caso de menina de seis anos de idade que manifestou esta sindrome apos quadro de gastroenterite. As manifestacoes clinicas alcancaram nadir5 com 15 dias de evolucao quando, devido a insuficiencia respiratoria, foi internada em unidade de terapia intensiva. Utilizaram-se altas doses de imunoglobulinas por via endovenosa (0,4/kg/dia) durante cinco dias, com excelente resultados. Sao relatadas as avaliacoes evolutivas, clinicas e eletromiograficas, no decorrer de seis meses, nao tendo ocorrido recidivas. As bases do tratamento atual da Sindrome de Guillain-Barre sao consideradas .
Asunto(s)
Humanos , Femenino , Niño , Inmunización Pasiva , Polirradiculoneuropatía/terapia , Campylobacter fetus/patogenicidad , Haemophilus influenzae/patogenicidadAsunto(s)
Lactante , Animales , Humanos , Masculino , Femenino , Campylobacter fetus/análisis , Infecciones por Campylobacter/epidemiología , Antígenos/aislamiento & purificación , Campylobacter fetus/aislamiento & purificación , Campylobacter fetus/patogenicidad , Serotipificación , Diarrea Infantil/etiologíaRESUMEN
Nueve niños con historia de diarrea crónica y coprocultivos positivos por Campylobacter fetus subespecie jejuni fueron admitidos y estudiados en el Hospital Nacional de Niños. Las edades variaron desde tres meses hasta tres años. Los pacientes provenían de pequeñas comunidades del área rural y de zonas urbanas. Previo a su ingreso los cuadros diarreicos se prolongaron por períodos desde treinta hasta ciento cincuenta días. En todos los casos se observó algún grado de desnutrición. Diarrea y vómito fueron los sintómas principales. Ninguno presentó el típico síndrome disenteriforme pero la presencia de muchos leucocitos en heces fue común a todos. Los cómputos de leucocitos oscilaron entre 5.000 y 20.000 con desviación a la izquierda. Intolerancia a la lactosa se documentó en dos casos. Ocho niños recibieron eritromicina y uno trimetoprín-sulfa, con rápida resolución de la diarrea. Todos los casos menos uno estuvieron asintomáticos antes del tercer día
Asunto(s)
Lactante , Preescolar , Humanos , Masculino , Femenino , Campylobacter fetus/patogenicidad , Infecciones por Campylobacter/complicaciones , Diarrea Infantil/etiologíaRESUMEN
Se presenta un caso de diarrea por triple etiología (Shigella Campylobacter-criptosporidium) en una niña de 3 meses y medio de edad, quién ingresó al Hospital Nacional de Niños en mayo de 1984