RESUMEN
OBJECTIVES: To evaluate the influence of violet LED, associated or not with a 17.5% hydrogen peroxide (HP) bleaching gel, on inflammation, mineralization in pulp tissue, and collagen fiber maturation in dentin and pulp tissue. MATERIALS AND METHODS: The maxillary molars of eighty Wistar rats were distributed into four groups (n = 10): CONT - without treatment; HP - 30 min application of 17.5% HP; LED - 20 min application of violet LED; and HP+LED - application of PH and violet LED. Rats were euthanized and jaws were processed for histologic and immunohistochemical evaluation (IL-17, IL-23, and osteocalcin) and picrosirius red immediately after (T0), and at 7 (T1), 15 (T2), and 30 days (T3) post-treatment, with Wilcoxon, Mann-Whitney, paired T-test, and T-test (α = 0.05). RESULTS: HP and HP+LED presented necrosis and severe inflammatory infiltrate. When compared to CONT group, LED presented severe osteocalcin (OCN) immunostaining in T2 and less immature fibers in T2 and T3. CONCLUSION: The violet LED caused no severe damage to the pulp tissue, increased IL-17 and IL-23 expression in T0 when associated with HP, and had no influence on pulp tissue mineralization, besides accelerating the maturation of collagen fibers of dentin. CLINICAL RELEVANCE: Violet LED therapy induced no inflammation in the pulp tissue of rats and played no role in pulp tissue fibrosis, besides accelerating the maturation of dentin collagen fibers.
Asunto(s)
Luces de Curación Dental , Pulpa Dental , Dentina , Peróxido de Hidrógeno , Inflamación , Fotoquimioterapia , Blanqueadores Dentales , Blanqueamiento de Dientes , Calcificación de Dientes , Animales , Colágeno/metabolismo , Pulpa Dental/efectos de los fármacos , Pulpa Dental/efectos de la radiación , Dentina/efectos de los fármacos , Dentina/efectos de la radiación , Geles , Peróxido de Hidrógeno/farmacología , Peróxido de Hidrógeno/uso terapéutico , Inflamación/tratamiento farmacológico , Inflamación/radioterapia , Interleucina-17/metabolismo , Interleucina-23/metabolismo , Osteocalcina/metabolismo , Fotoquimioterapia/métodos , Ratas , Ratas Wistar , Blanqueamiento de Dientes/métodos , Blanqueadores Dentales/farmacología , Blanqueadores Dentales/uso terapéutico , Calcificación de Dientes/efectos de los fármacos , Calcificación de Dientes/efectos de la radiaciónRESUMEN
OBJECTIVE: This study evaluated the effects of fluoride varnishes containing sodium trimetaphosphate (TMP) on bovine enamel demineralization in vitro. MATERIAL AND METHODS: Enamel bovine discs were randomly assigned into six groups (n = 20/group): placebo, 2.5% NaF, 2.5% NaF/5% TMP, 5% NaF, 5% NaF/5% TMP, and a commercial formulation (Duraphat, 5% NaF). Varnishes were applied on all enamel discs and kept for 6 h. Loosely and firmly bound fluoride formed on/in enamel after treatment were analyzed in 10 discs from each group. The other 10 discs were subjected to a pH-cycling regimen for 7 days, and analyzed for surface (SH) and cross-sectional hardness (ΔKHN), as well as for loosely and firmly bound fluoride in/on enamel. Data were analyzed by analysis of variance (ANOVA) followed by Student-Newman-Keuls' test (p < .05). RESULTS: The lowest SH change and ΔKHN were observed for the 5%NaF/5%TMP varnish, which was significantly different from all the other groups. Both fluoridated varnishes containing TMP promoted significantly lower SH change and ΔKHN when compared with their counterparts without TMP. Loosely and firmly bound fluoride was significantly lower in groups treated with varnishes containing TMP. CONCLUSION: TMP and fluoride added to varnishes have a synergistic effect against enamel demineralization in vitro.
Asunto(s)
Cariostáticos/farmacología , Esmalte Dental/efectos de los fármacos , Fluoruros Tópicos/farmacología , Fluoruro de Sodio/farmacología , Calcificación de Dientes/efectos de los fármacos , Animales , Cariostáticos/uso terapéutico , Bovinos , Relación Dosis-Respuesta a Droga , Fluoruros/administración & dosificación , Fluoruros/farmacología , Fluoruros Tópicos/administración & dosificación , Fosfatos/farmacología , Fluoruro de Sodio/administración & dosificación , Desmineralización DentalRESUMEN
Materials such as calcium hydroxide paste and mineral trioxide aggregate are used in apexification treatment of immature permanent teeth, but the search for improved materials with higher characteristics of biocompatibility results in different materials. Biodentine is a tricalcium silicate cement that possesses adequate handling characteristics and acceptable mechanical and bioactivity properties. This report describes the case of a 9-year-old boy who was referred to the Department of Dental Clinic of Querétaro Autonomous University of Mexico. One month prior the patient had suffered a dental trauma of his upper left central incisor and had been treated by another dentist. The clinical diagnosis was previously initiated therapy and symptomatic apical periodontitis. The treatment was apexification with Biodentine. At follow-ups performed at 3, 6, and 18 months after treatment the tooth was asymptomatic. The cone-beam computed tomography scan at 18-month postoperative follow-up revealed continuity of periodontal ligament space, absence of periapical rarefactions, and a thin layer of calcified tissue formed apical to the Biodentine barrier. On the basis of sealing ability and biocompatibility, apexification treatment with Biodentine was applied in the present case report. The favorable clinical and radiographic outcome in this case demonstrated that Biodentine may be an efficient alternative to the conventional apexification materials.
Asunto(s)
Apexificación/métodos , Compuestos de Calcio/uso terapéutico , Silicatos/uso terapéutico , Ápice del Diente/efectos de los fármacos , Compuestos de Aluminio/uso terapéutico , Hidróxido de Calcio/uso terapéutico , Niño , Tomografía Computarizada de Haz Cónico , Necrosis de la Pulpa Dental/terapia , Combinación de Medicamentos , Humanos , Incisivo/diagnóstico por imagen , Incisivo/lesiones , Masculino , México , Óxidos/uso terapéutico , Periodontitis Periapical/tratamiento farmacológico , Ligamento Periodontal/patología , Materiales de Obturación del Conducto Radicular/uso terapéutico , Irrigantes del Conducto Radicular/uso terapéutico , Preparación del Conducto Radicular/métodos , Tratamiento del Conducto Radicular/métodos , Ápice del Diente/diagnóstico por imagen , Calcificación de Dientes/efectos de los fármacosRESUMEN
INTRODUCTION: This study evaluated the effect of hypertension on tissue response to and mineralization capacity of white and gray mineral trioxide aggregate (MTA) (Angelus Industry Ontological Products, Londrina, Brazil), an endodontic reparative cement. METHODS: Polyethylene tubes containing gray MTA, white MTA, or intermediate restorative material (positive control) or an empty tube (negative control) were implanted into the dorsal connective tissue of spontaneous hypertensive and Wistar rats (n = 12 each). Six rats in each group were sacrificed after 7 days, and the remainder after 30 days. Tubes with surrounding tissue were removed, and a histologic analysis was performed using hematoxylin-eosin and von Kossa staining and examination by polarized light microscopy. RESULTS: The inflammatory response to all materials was greater in hypertensive compared with normotensive rats (P < .05). Positive von Kossa staining and birefringent structures in polarized light were observed for both gray and white MTA (P > .05), but these were more pronounced in normotensive rats (P < .05). Necrotic areas with positive von Kossa staining were observed for intermediate restorative material. CONCLUSIONS: Hypertension undermines tissue repair and mineralization, which can negatively affect treatment outcome. Nonetheless, mineralization in response to MTA was observed even under hypertensive conditions.
Asunto(s)
Compuestos de Aluminio/farmacología , Compuestos de Calcio/farmacología , Cementos Dentales/farmacología , Hipertensión/metabolismo , Óxidos/farmacología , Materiales de Obturación del Conducto Radicular/farmacología , Silicatos/farmacología , Calcificación de Dientes/efectos de los fármacos , Animales , Materiales Biocompatibles/farmacología , Calcio/metabolismo , Tejido Conectivo/efectos de los fármacos , Tejido Conectivo/patología , Combinación de Medicamentos , Cementos de Ionómero Vítreo/farmacología , Hipertensión/patología , Masculino , Necrosis , Ratas , Ratas Wistar , Tejido Subcutáneo/efectos de los fármacos , Resultado del TratamientoRESUMEN
OBJECTIVE: The purpose of this study was to evaluate surface roughness and changes in the composition of enamel submitted to different bleaching protocols and toothbrushing with regular and whitening toothpastes. BACKGROUND DATA: Bleaching treatment could promote morphological and chemical changes in enamel surface. METHODS: Enamel blocks were randomized into nine groups (n=10) according to the bleaching treatment (no bleaching, control group; 6% hydrogen peroxide, HP; or 10% carbamide peroxide, CP) and toothpaste used (placebo, PL; regular, R; or whitening dentifrice, W). Bleaching was performed according to manufacturers' instructions and all groups were submitted to 30,000 cycles of simulated toothbrushing with toothpaste (PL, R, or W). Mineral content evaluation and enamel roughness were evaluated initially (T1), after bleaching (T2), and after toothbrushing (T3), using an energy-dispersive micro X-ray fluorescence spectrometer and profilometry, respectively. Data were statistically analyzed with two way ANOVA, Tukey, and Dunnett tests (5%). RESULTS: Enamel surface roughness was influenced by bleaching and toothbrushing. Surface roughness increased for the groups that brushed with the placebo dentifrice (CP+PL, HP+PL, C+PL) and for the control group that brushed with whitening dentifrice (C+W). Enamel Ca/P ratio decreased after bleaching, but toothbrushing, regardless of the dentifrice used, did not reduce the enamel mineral content. CONCLUSIONS: The bleaching treatment resulted in a decrease of enamel mineral content, but the studied dentifrices did not contribute to surface mineral loss.
Asunto(s)
Esmalte Dental/efectos de los fármacos , Esmalte Dental/efectos de la radiación , Dentífricos/farmacología , Terapia por Luz de Baja Intensidad , Blanqueadores Dentales/farmacología , Blanqueamiento de Dientes , Humanos , Propiedades de Superficie/efectos de los fármacos , Propiedades de Superficie/efectos de la radiación , Técnicas de Cultivo de Tejidos , Calcificación de Dientes/efectos de los fármacos , Calcificación de Dientes/efectos de la radiación , Cepillado DentalRESUMEN
Immature avulsed teeth are not usually treated with pulp revascularization because of the possibility of complications. However, this therapy has shown success in the treatment of immature teeth with periapical lesions. This report describes the case of an immature replanted tooth that was successfully treated by pulp revascularization. An 8-year-old boy suffered avulsion on his maxillary left lateral incisor. The tooth showed incomplete root development and was replanted after 30 minutes. After diagnosis, revascularization therapy was performed by irrigating the root canal and applying a calcium hydroxide paste and 2% chlorhexidine gel for 21 days. In the second session, the intracanal dressing was removed and a blood clot was stimulated up to the cervical third of the root canal. Mineral trioxide aggregate was placed as a cervical barrier at the entrance of the root canal and the crown was restored. During the follow-up period, periapical repair, apical closure and calcification in the apical 4 mm of the root canal was observed. An avulsed immature tooth replanted after a brief extra-alveolar period and maintained in a viable storage medium may be treated with revascularization.
Asunto(s)
Apexificación/métodos , Incisivo/lesiones , Avulsión de Diente/terapia , Reimplante Dental/métodos , Compuestos de Aluminio/uso terapéutico , Compuestos de Calcio/uso terapéutico , Hidróxido de Calcio/uso terapéutico , Niño , Clorhexidina/uso terapéutico , Pulpa Dental/irrigación sanguínea , Pulpa Dental/efectos de los fármacos , Cavidad Pulpar/efectos de los fármacos , Combinación de Medicamentos , Estudios de Seguimiento , Humanos , Masculino , Óxidos/uso terapéutico , Materiales de Obturación del Conducto Radicular/uso terapéutico , Irrigantes del Conducto Radicular/uso terapéutico , Preparación del Conducto Radicular/métodos , Silicatos/uso terapéutico , Ápice del Diente/efectos de los fármacos , Calcificación de Dientes/efectos de los fármacosRESUMEN
OBJECTIVE: This study analyzed the effects of a fluoride (F) varnish supplemented with sodium trimetaphosphate (TMP) on the remineralization of caries-like lesions in situ. MATERIALS AND METHODS: Twelve subjects used palatal devices with demineralized enamel discs for 3 days, following a double-blind, crossover protocol. Test groups included placebo (no F or TMP), 5% NaF and 5% NaF/5% TMP varnishes. The percentage of surface hardness recovery (%SHR) and cross-sectional hardness (ΔKHN) were determined. RESULTS: Significant differences were observed among all varnishes regarding %SHR and ΔKHN. The highest %SHR and the lowest ΔKHN were seen for the 5% NaF/5% TMP varnish, followed by 5% NaF and placebo. CONCLUSION: The remineralizing effect of a 5% NaF varnish is significantly enhanced when associated with TMP. CLINICAL RELEVANCE: The reduction in the subsurface lesion area of enamel treated with the TMP-containing varnish implies that cavities would take longer to develop or might not develop at all depending on individual factors, resulting in lower net caries increments at individual and population levels.
Asunto(s)
Caries Dental/tratamiento farmacológico , Caries Dental/metabolismo , Fluoruros Tópicos/administración & dosificación , Polifosfatos/administración & dosificación , Calcificación de Dientes/efectos de los fármacos , Adulto , Método Doble Ciego , Femenino , Humanos , MasculinoRESUMEN
INTRODUCTION: Pulp necrosis in immature teeth and the resulting periodontal apical inflammation negatively affect root formation. Resolvin E1 (RvE1) is a lipid-derived endogenous pro-resolution molecule that controls inflammation. The aim of this investigation was to evaluate the impact of RvE1 applied as an intracanal medication on root formation in nonvital immature teeth. METHODS: To arrest root development, pulpectomy was performed in the lower first molars of 4-week-old Wistar rats. After 3 weeks, irrigation with 2.5% sodium hypochlorite and 0.9% sterile saline was performed, and either a triple antibiotic paste (TAP) or RvE1 in saline was applied into the root canals. In the control group, access openings drilled into molars were left exposed to the oral environment. Root development and periapical repair were evaluated radiographically and histologically at 3 and 6 weeks after treatment. RESULTS: RvE1 reduced periapical lesion size compared with the control at 3 weeks, which was similar to TAP. Inflammatory response in the RvE1-treated group was markedly reduced compared with both TAP and control specimens. At 6 weeks, root development was observed in both groups, but RvE1 treatment produced less cellularity with more regular calcified tissue deposition. CONCLUSIONS: RvE1 and TAP had a positive impact on reducing inflammation and promoting root formation. RvE1 was more effective in reducing inflammation at earlier stages. RvE1 has potential to be used as root canal dressing to control inflammation in endodontically compromised teeth before complete root formation. Stability of RvE1 within the root canal and its delivery are issues to be addressed before its clinical use.
Asunto(s)
Antiinflamatorios/uso terapéutico , Cavidad Pulpar/efectos de los fármacos , Necrosis de la Pulpa Dental/tratamiento farmacológico , Ácido Eicosapentaenoico/análogos & derivados , Irrigantes del Conducto Radicular/uso terapéutico , Raíz del Diente/efectos de los fármacos , Diente no Vital/tratamiento farmacológico , Animales , Antibacterianos/uso terapéutico , Antiinflamatorios/administración & dosificación , Ciprofloxacina/uso terapéutico , Ácido Eicosapentaenoico/administración & dosificación , Ácido Eicosapentaenoico/uso terapéutico , Masculino , Metronidazol/uso terapéutico , Minociclina/uso terapéutico , Odontogénesis/efectos de los fármacos , Periodontitis Periapical/terapia , Pulpectomía/métodos , Ratas , Ratas Wistar , Hipoclorito de Sodio/uso terapéutico , Calcificación de Dientes/efectos de los fármacos , Raíz del Diente/crecimiento & desarrolloRESUMEN
The aim of this study was to evaluate the odontogenic potential of undifferentiated pulp cells (OD-21 cell line) through chemical stimuli in vitro. Cells were divided into uninduced cells (OD-21), induced cells (OD-21 cultured in supplemented medium/OD-21+OM) and odontoblast-like cells (MDPC-23 cell line). After 3, 7, 10 and 14 days of culture, it was evaluated: proliferation and cell viability, alkaline phosphatase activity, total protein content, mineralization, immunolocalization of dentin matrix acidic phosphoprotein 1 (DMP1), alkaline phosphatase (ALP) and osteopontin (OPN) and quantification of genes ALP, OSTERIX (Osx), DMP1 and runt-related transcription factor 2 (RUNX2) through real-time polymerase chain reaction (PCR). Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests (p<0.05). There was a decrease in cell proliferation in OD-21 + OM, whereas cell viability was similar in all groups, except at 7 days. The amount of total protein was higher in group OD-21 + OM in all periods; the same occurred with ALP activity after 10 days when compared with OD-21, with no significant differences from the MDPC-23 group. Mineralization was higher in OD-21+OM when compared with the negative control. Immunolocalization demonstrated that DMP1 and ALP were highly expressed in MDPC-23 cells and OD-21 + OM cells, whereas OPN was high in all groups. Real-time PCR revealed that DMP1 and ALP expression was higher in MDPC-23 cell cultures, whereas RUNX2 was lower for these cells and higher for OD-21 negative control. Osx expression was lower for OD-21 + OM. These results suggest that OD-21 undifferentiated pulp cells have odontogenic potential and could be used in dental tissue engineering.
Asunto(s)
Pulpa Dental/citología , Odontogénesis/fisiología , Fosfatasa Alcalina/análisis , Animales , Recuento de Células , Técnicas de Cultivo de Célula , Diferenciación Celular/efectos de los fármacos , Línea Celular , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Subunidad alfa 1 del Factor de Unión al Sitio Principal/análisis , Medios de Cultivo , Proteínas de la Matriz Extracelular/análisis , Ratones , Odontoblastos/efectos de los fármacos , Osteopontina/análisis , Fosfoproteínas/análisis , Proteínas/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factor de Transcripción Sp7 , Factores de Tiempo , Calcificación de Dientes/efectos de los fármacos , Factores de Transcripción/análisisRESUMEN
INTRODUCTION: The main purpose of this study was to evaluate the biocompatibility and bioactivity of a new mineral trioxide aggregate (MTA)-based endodontic sealer, MTA Fillapex (MTA-F; Angelus, Londrina, Brazil), in human cell culture. METHODS: Human osteoblast-like cells (Saos-2) were exposed for 1, 2, 3, and 7 days to MTA-F, Epiphany SE (EP-SE; SybronEndo, Orange, CA), and zinc oxide-eugenol sealer (ZOE). Unexposed cultures were the control group (CT). The viability of the cells was assessed by MTT assay and the morphology by scanning electron microscopy (SEM). The bioactivity of MTA-F was evaluated by alkaline phosphatase activity (ALP) and the detection of calcium deposits in the culture with alizarin red stain (ARS). Energy-dispersive X-ray spectroscopy (EDS) was used to chemically characterize the hydroxyapatite crystallites (HAP). Saos-2 cells were cultured for 21 days for ARS and SEM/EDS. ARS results were expressed as the number of stained nodules per area. Statistical analysis was performed with analysis of variance and Bonferroni tests (P < .01). RESULTS: MTA-F exposure for 1, 2, and 3 days resulted in increased cytotoxicity. In contrast, viability increased after 7 days of exposure to MTA-F. Exposure to EP-SE and ZOE was cytotoxic at all time points. At day 7, ALP activity increase was significant in the MTA-F group. MTA-F presented the highest percentage of ARS-stained nodules (MTA-F > CT > EP-SE > ZOE). SEM/EDS analysis showed hydroxyapatite crystals only in the MTA-F and CT groups. In the MTA-F group, crystallite morphology and chemical composition were different from CT. CONCLUSIONS: After setting, the cytotoxicity of MTA-F decreases and the sealer presents suitable bioactivity to stimulate HAP crystal nucleation.
Asunto(s)
Compuestos de Aluminio/farmacología , Compuestos de Calcio/farmacología , Durapatita/química , Osteoblastos/efectos de los fármacos , Óxidos/farmacología , Materiales de Obturación del Conducto Radicular/farmacología , Silicatos/farmacología , Calcificación de Dientes/efectos de los fármacos , Compuestos de Aluminio/síntesis química , Compuestos de Aluminio/química , Compuestos de Aluminio/toxicidad , Análisis de Varianza , Compuestos de Calcio/síntesis química , Compuestos de Calcio/química , Compuestos de Calcio/toxicidad , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Cristalización , Combinación de Medicamentos , Humanos , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Óxidos/síntesis química , Óxidos/química , Óxidos/toxicidad , Materiales de Obturación del Conducto Radicular/síntesis química , Materiales de Obturación del Conducto Radicular/química , Materiales de Obturación del Conducto Radicular/toxicidad , Silicatos/síntesis química , Silicatos/química , Silicatos/toxicidad , Espectrometría por Rayos X , Estadísticas no ParamétricasRESUMEN
The aim of this study was to evaluate the odontogenic potential of undifferentiated pulp cells (OD-21 cell line) through chemical stimuli in vitro. Cells were divided into uninduced cells (OD-21), induced cells (OD-21 cultured in supplemented medium/OD-21+OM) and odontoblast-like cells (MDPC-23 cell line). After 3, 7, 10 and 14 days of culture, it was evaluated: proliferation and cell viability, alkaline phosphatase activity, total protein content, mineralization, immunolocalization of dentin matrix acidic phosphoprotein 1 (DMP1), alkaline phosphatase (ALP) and osteopontin (OPN) and quantification of genes ALP, OSTERIX (Osx), DMP1 and runt-related transcription factor 2 (RUNX2) through real-time polymerase chain reaction (PCR). Data were analyzed by Kruskal-Wallis and Mann-Whitney U tests (p<0.05). There was a decrease in cell proliferation in OD-21 + OM, whereas cell viability was similar in all groups, except at 7 days. The amount of total protein was higher in group OD-21 + OM in all periods; the same occurred with ALP activity after 10 days when compared with OD-21, with no significant differences from the MDPC-23 group. Mineralization was higher in OD-21+OM when compared with the negative control. Immunolocalization demonstrated that DMP1 and ALP were highly expressed in MDPC-23 cells and OD-21 + OM cells, whereas OPN was high in all groups. Real-time PCR revealed that DMP1 and ALP expression was higher in MDPC-23 cell cultures, whereas RUNX2 was lower for these cells and higher for OD-21 negative control. Osx expression was lower for OD-21 + OM. These results suggest that OD-21 undifferentiated pulp cells have odontogenic potential and could be used in dental tissue engineering.
O objetivo foi avaliar o potencial odontogênico de células indiferenciadas da polpa (OD-21) por meio de indução química in vitro. As células foram divididas em grupos: controle (OD-21), induzido (OD-21 em meio suplementado/OD-21 + OM), e células odontoblastóides (MDPC-23). Após 3, 7, 10 e 14 dias, avaliou-se proliferação e viabilidade celular, proteína total e fosfatase alcalina (ALP), mineralização, imunolocalização da proteína da matriz dentinária 1 (DMP1), ALP e osteopontina (OPN), assim como a expressão dos genes ALP, OSTERIX (Osx), DMP1 e fator de transcrição RUNX2 por PCR em tempo real. Os dados foram avaliados pelo teste de Kruskal-Wallis seguido pelo teste de Mann-Whitney U (p<0.05). Houve diminuição na proliferação celular em OD-21 + OM, com viabilidade celular similar em todos os grupos, exceto aos sete dias. O conteúdo de proteína total foi maior no grupo OD-21 + OM em todos os períodos; o mesmo ocorreu com a atividade de ALP quando comparada com o grupo OD-21, além de apresentar resultados similares ao grupo MDPC-23. A mineralização foi maior em OD-21 + OM quando comparada com o controle negativo. A imunolocalização demonstrou expressão de DMP1 e ALP em MDPC-23 e OD-21 + OM, enquanto que todos os grupos foram positivos para OPN. A expressão gênica de DMP1 e ALP foi maior nas culturas de MDPC-23, enquanto que a de RUNX2 foi menor para estas células e maior no controle negativo. A expressão de OSTERIX foi menor em OD-21 + OM quando comparada aos outros grupos. Sugere-se que as células indiferenciadas da polpa da linhagem OD-21 apresentam potencial odontogênico e poderiam ser usadas para a engenharia tecidual.
Asunto(s)
Animales , Ratones , Pulpa Dental/citología , Odontogénesis/fisiología , Fosfatasa Alcalina/análisis , Recuento de Células , Técnicas de Cultivo de Célula , Línea Celular , Medios de Cultivo , Diferenciación Celular/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Subunidad alfa 1 del Factor de Unión al Sitio Principal/análisis , Proteínas de la Matriz Extracelular/análisis , Odontoblastos/efectos de los fármacos , Osteopontina/análisis , Fosfoproteínas/análisis , Proteínas/análisis , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de Tiempo , Calcificación de Dientes/efectos de los fármacos , Factores de Transcripción/análisisRESUMEN
AIM: Our aim was to test the hypothesis that co-exposure to lead and fluoride alter the severity of enamel fluorosis. MATERIALS AND METHODS: Wistar rats were allocated in four groups: control, and 3 groups that received water containing 100 ppm of fluoride (F), 30 ppm of lead (Pb), or 100 ppm of F and 30 ppm of Pb (F+Pb) from the beginning of gestation. Enamel analysis and F and Pb determinations in enamel, dentine, and bone were performed in 81-day-old animals. Fluorosis was quantified using a new fluorosis index based on the identification of incisor enamel defects (white bands and white islets, representing hypomineralization, and cavities) weighted according to their severity and quantity. Hypomineralization was validated histopathologically by polarizing microscopy and microradiography. Scores were given by two blinded calibrated examiners (intra and interexaminer kappa values were 0.8 and 0.86, respectively). RESULTS: The control and the Pb groups presented normal enamel. The F+Pb group presented more severe enamel defects compared with the F group (P<0.0001). CONCLUSIONS: This study shows that lead exacerbates dental fluorosis in rodents, suggesting that co-exposure to lead may affect the degree of fluorosis.
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Cariostáticos/efectos adversos , Exposición a Riesgos Ambientales , Fluoruros/efectos adversos , Fluorosis Dental/etiología , Plomo/efectos adversos , Animales , Cariostáticos/análisis , Caries Dental/inducido químicamente , Caries Dental/patología , Esmalte Dental/química , Dentina/química , Sinergismo Farmacológico , Femenino , Fémur/química , Fluoruros/análisis , Fluorosis Dental/clasificación , Fluorosis Dental/patología , Incisivo/química , Plomo/análisis , Plomo/sangre , Masculino , Microrradiografía , Microscopía de Polarización , Fósforo/análisis , Distribución Aleatoria , Ratas , Ratas Wistar , Calcificación de Dientes/efectos de los fármacos , Desmineralización Dental/inducido químicamente , Desmineralización Dental/clasificación , Desmineralización Dental/patología , Abastecimiento de Agua/análisisRESUMEN
BACKGROUND: The difference between dental age (DA) and chronological age (CA) indicates an advance or delay in comparison with the normal standard. Considering that DA has a very close correlation with CA in a developing child, this study aimed to investigate the relationship between the effects of antiretroviral therapy on the development of HIV positive children, by observing the timing of dental mineralization. METHODS: A retrospective examination was made of the medical records and dental panoramic radiographs of 50 HIV-positive pediatric patients, aged 37-168 months of age. Through these radiographs, their DA was estimated and compared with their CA. RESULTS: The mean DA was significantly lower than the mean CA in the entire studied sample (P < 0.01). There was a statistical difference between children who received antiretroviral drugs and those who used no drugs (P = 0.02), i.e. the non-treated individuals presented practically no difference between CA and DA, while the treated patients showed a difference of 10.67 months. CONCLUSION: We conclude that the DA of HIV infected children was delayed when compared to the CA, and there was a positive association between the use of antiretroviral therapy and a delay in the chronology of dental mineralization in the sample.
Asunto(s)
Antirretrovirales/efectos adversos , Seropositividad para VIH/tratamiento farmacológico , Calcificación de Dientes/efectos de los fármacos , Adolescente , Determinación de la Edad por los Dientes , Factores de Edad , Fármacos Anti-VIH/efectos adversos , Terapia Antirretroviral Altamente Activa , Niño , Preescolar , Femenino , Inhibidores de la Proteasa del VIH/efectos adversos , Humanos , Masculino , Odontogénesis/efectos de los fármacos , Radiografía Panorámica , Estudios Retrospectivos , Inhibidores de la Transcriptasa Inversa/efectos adversosRESUMEN
INTRODUCTION: Recently, it was shown that the interaction of each of mineral trioxide aggregate (MTA) and Portland cement with dentin in phosphate-buffered saline (PBS) promotes a biomineralization process that leads to the formation of an interfacial layer with tag-like structures at the cement-dentin interface. This study analyzes the influence of the biomineralization process on the push-out strength of ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), MTA Branco (Angelus Soluções Odontológicas, Londrina, PR, Brazil), MTA BIO (Angelus Soluções Odontológicas), or Portland cement with and without calcium chloride. METHODS: Dentin discs with standardized cavities were filled with ProRoot MTA, MTA Branco, MTA BIO, white Portland cement + 20% bismuth oxide (PC1), or PC1 + 10% of calcium chloride (PC2). The specimens were randomly divided into two groups: cement in contact with a wet cotton pellet for 72 hours or immersed in PBS for 2 months. The bond strengths were measured with the Instron Testing machine (Model 4444; Instron Corp, Canton, MA), and the fractured surfaces on the root walls were observed by scanning electron microscopy. RESULTS: All samples immersed in PBS displayed a significantly greater resistance to displacement than that observed for the samples in contact with a wet cotton pellet for 72 hours (p < 0.05). MTAs displayed a significantly greater resistance to displacement than Portland cements. CONCLUSION: It was concluded that the biomineralization process positively influenced the push-out bond strength of the cements, particularly the MTA groups.
Asunto(s)
Cementos Dentales/farmacología , Desconsolidación Dental , Adaptación Marginal Dental , Dentina/efectos de los fármacos , Materiales de Obturación del Conducto Radicular/farmacología , Compuestos de Aluminio/farmacología , Materiales Biocompatibles/farmacología , Compuestos de Calcio/farmacología , Análisis del Estrés Dental , Dentina/ultraestructura , Combinación de Medicamentos , Humanos , Ensayo de Materiales , Óxidos/farmacología , Silicatos/farmacología , Propiedades de Superficie , Calcificación de Dientes/efectos de los fármacosRESUMEN
The emphasis currently given to new technologies for enamel remineralization suggests that the changes in the understanding of the dental caries disease, which occurred in the last century, were either not yet adopted or were forgotten. Just like in the past, when the disease was "treated" by restoring cavities, there is presently a misunderstanding on the concept of incipient lesion remineralization. The aim of this paper was to review some concepts about caries, the natural phenomenon of enamel remineralization and the effect of fluoride (F) on it, and also to discuss the clinical relevance of remineralizing products recently launched in the marketplace aiming to "treat early caries lesions".
Asunto(s)
Cariostáticos/uso terapéutico , Caries Dental/prevención & control , Esmalte Dental/fisiología , Fluoruros/uso terapéutico , Calcificación de Dientes/fisiología , Fosfatos de Calcio/farmacología , Fosfatos de Calcio/uso terapéutico , Cariostáticos/farmacología , Caries Dental/etiología , Caries Dental/patología , Esmalte Dental/efectos de los fármacos , Placa Dental/patología , Placa Dental/prevención & control , Progresión de la Enfermedad , Fluoruros/farmacología , Humanos , Calcificación de Dientes/efectos de los fármacosRESUMEN
The emphasis currently given to new technologies for enamel remineralization suggests that the changes in the understanding of the dental caries disease, which occurred in the last century, were either not yet adopted or were forgotten. Just like in the past, when the disease was "treated" by restoring cavities, there is presently a misunderstanding on the concept of incipient lesion remineralization. The aim of this paper was to review some concepts about caries, the natural phenomenon of enamel remineralization and the effect of fluoride (F) on it, and also to discuss the clinical relevance of remineralizing products recently launched in the marketplace aiming to "treat early caries lesions".
Asunto(s)
Humanos , Cariostáticos/uso terapéutico , Caries Dental/prevención & control , Esmalte Dental/fisiología , Fluoruros/uso terapéutico , Calcificación de Dientes/fisiología , Fosfatos de Calcio/farmacología , Fosfatos de Calcio/uso terapéutico , Cariostáticos/farmacología , Progresión de la Enfermedad , Caries Dental/etiología , Caries Dental/patología , Esmalte Dental/efectos de los fármacos , Placa Dental/patología , Placa Dental/prevención & control , Fluoruros/farmacología , Calcificación de Dientes/efectos de los fármacosRESUMEN
The aim of this study was to evaluate the remineralization of incipient carious lesions in bovine enamel in situ. Artificial carious lesions were produced and fixed in removable lower appliances in the region of the lingual surfaces of first molars, in six volunteers with ages between 18 and 22 years, who were subjected to 3 distinct experimental periods of 1 week each. In the first period (control group), patients brushed their teeth with a non-fluoridated dentifrice 4 times a day (after meals), and, in the second period (group I), patients used a dentifrice containing 1,500 ppm of fluorine (in the form of MFP). In the third period (group II) volunteers brushed their teeth with non-fluoridated dentifrice and used chewing gum containing 60% of sucrose during 20 minutes, 4 times a day (after meals). Before and after each treatment, the specimens underwent Vicker's hardness test (200 g of load), and the remineralization percentage (alpha) was calculated. The control group showed 2.78% of demineralization, and groups I and II showed 3.36 and 5.21% of remineralization, respectively. Statistical analysis (with Kruskal-Wallis and Miller's tests) showed significant difference (p < 0.05) between the control and experimental groups (I and II). Group II showed greater alpha than group I, but this difference was not significant. These results suggest that the use of sucrose-containing chewing gum and fluoridated dentifrice has a considerable effect on the remineralization of incipient carious lesions and may be a valuable alternative for their prevention.
Asunto(s)
Goma de Mascar , Caries Dental/tratamiento farmacológico , Esmalte Dental/efectos de los fármacos , Dentífricos , Fluoruros/farmacología , Sacarosa/farmacología , Calcificación de Dientes/efectos de los fármacos , Remineralización Dental , Adolescente , Adulto , Animales , Humanos , SalivaRESUMEN
The present study reveals the inhibitory effect of iron intoxication on the process of dentine mineralization. Wistar rats were injected intraperitoneally with iron dextran at 0.88 g/kg body weight per day for 10 days during the period of odontogenesis. An age-matched group was injected intraperitoneally with bisodium etidronate (EHBP) at 20 mg/kg body weight per day for 10 days. Another age-matched group was treated with similar amounts of saline intraperitoneally and considered as control. At the end of the experimental period the animals intoxicated with iron exhibited non-mineralized dentine and mineralized bone. The animals treated with EHBP showed non-mineralized dentine and bone. These findings would suggest the existence of different mineralization mechanisms for bone and dentine.
Asunto(s)
Dentina/fisiopatología , Sobrecarga de Hierro/fisiopatología , Calcificación de Dientes/fisiología , Proceso Alveolar/efectos de los fármacos , Proceso Alveolar/fisiopatología , Animales , Calcificación Fisiológica/efectos de los fármacos , Calcificación Fisiológica/fisiología , Dentina/efectos de los fármacos , Ácido Etidrónico/farmacología , Hematínicos/farmacología , Inyecciones Intraperitoneales , Complejo Hierro-Dextran/farmacología , Ratas , Ratas Wistar , Calcificación de Dientes/efectos de los fármacosRESUMEN
In most animal species, acute fluorosis is triggered by the accidental consumption of substances with a high content of fluorine, widely used in pest control. Fluorine normally concentrates in bones and teeth, and forms nearly 0.05% of these structures in mature animals, positively contributing to increase resistance of enamel to caries and favoring mineralization after eruption, a process which increases with age. The present article submits a form of study of fluorosis, stressing due warning as to its harmful effects.
Asunto(s)
Modelos Animales de Enfermedad , Fluorosis Dental , Fluoruro de Sodio/efectos adversos , Calcificación de Dientes/efectos de los fármacos , Animales , Fluoruros/análisis , Mamíferos , Ratas , Diente/químicaRESUMEN
Degree of mineralization of enamel is one of the parameters mediating in its resistance to acid dissolution and at the stage of teething, the enamel is immature and, therefore, present a higher susceptibility to dental caries. This investigation was carried out with 38 preschool children (means = 5.5 years) and 19 children coursing second grade (means = 7.6 years). They were treated with 10 oral rinsing with mineralizing solution in order to evaluate its effect on enamel resistance to acid dissolution; colorimetric technique was used for such purpose. A study in vitro was also performed in impacted third molars extracted by surgery and to two halves of the clinical crowns of such molars the treatment was applied; the other two halves were taken as control. Increase of enamel resistance found was 18.6% in the study of preschool children and 34.6% in the children attending to second grade school. In both groups, 89.5% of the children increased enamel resistance and in the study in vitro increaser enamel was 14.9%.. It is concluded that the use of risings with mineralizing solution at the outbreak of permanent teething increase, significatively, enamel resistance, and that at the age of seven the enamel is still immature, thus its resistance to acid dissolution can be also increased using mineralizing solution.