RESUMEN

This study aimed at investigating the effects of broccoli residues fermented with probiotics (BF) on the growth performance, immunity, and gut health in broilers challenged with Clostridium perfringens (C. perfringens). A total of 600 broilers (one day old) were randomly allotted into five treatments with six replicates of 20 birds each and were reared until 42 days of age. The treatments included a positive control (PC, fed a basal diet and reared on uncontaminated litter), a negative control (NC, birds reared on litter contaminated with C. perfringens and fed a basal diet), and NC plus BF at 25, 50 or 75 g/kg of diet. The BF contained yeast 3.1 × 10 (7) cfu/g, lactic acid bacteria 9.5 × 10 (6) cfu/g and Bacillus subtilis 3.5 × 10 (6) cfu/g. Birds in the NC group showed lower (p<0.05) feed intake and body weight gain, whereas BF supplementation recovered (p<0.05) the growth performance to the levels of PC group. Dietary BF at 50and 75 g/kg reduced (p<0.05) broiler mortality. Similarly, compared to the NC group, BF increased (p<0.05) immune organ weights and serum immunoglobulins A, G, and M to the levels of PC group. The ileal populations of Escherichia coli and Gram-negative bacteria were decreased (p<0.05) by BF to the levels of PC, and C. perfringens was also decreased (p<0.05) by BF. The serum profiles of mono- and di-amine oxidase were decreased (p<0.05) by BF. BF at 75 g/kg reduced (p<0.05) monoamine oxidase compared with the other BF doses. The results suggest that broccoli residues fermented with probiotics can be a novel biological feed additive to protect the performance and health of broilers against C. perfringens infection.

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RESUMEN

Devido à alta frequência de lesões em membros locomotores e sua difícil cicatrização em equinos, há uma crescente busca por tratamentos alternativos e fitoterápicos, visando redução de custo e de fácil manipulação. O repolho vem sendo utilizado com êxito devido a propriedades adstringente e de regeneração tecidual, além de promover a proliferação de fibrosblastos e motilidade das células epiteliais na região do tecido lesionado. O óleo de Girassol ozonizado possui característica de penetrar nos tecidos, ocasionar homeostasia, e contribuir com ação bactericida, fungicida e aumentar fibrinogênio na lesão. Neste relato de caso, após o tratamento como Repolho e óleo de Girassol ozonizado, o equino obteve alta hospitalar em boas condições clínicas e a ferida em processo final de cicatrização.

Due to the high frequency of lesions on locomotor limbs and their difficult healing in equines, there is a growing search for alternative and phytotherapeutic treatments, aiming at cost reduction and easy manipulation. The cabbage has been used successfully due to abstergent and tissue regeneration properties, besides promoting fibroblast proliferation and epithelial cell motility in the region of the injured tissue. The ozonized sunflower oil has the characteristic of penetrating the tissues, causing homeostasis, and contributing with bactericidal, fungicidal action and increasing fibrinogen in the lesion. In this case report, after treatment with Cabbage and ozonized Sunflower oil, the horse was discharged in good clinical condition and the wound in the final healing process.

Debido la grande frecuencia de las lesiones en los membros de los equídeos y la dificultad de cicatrización de los mismo hay una búsqueda creciente por tratamentos alternativos y de fitoterapia, com el objetivo de reducir los gastos y para que sea fácil su manipulación. El repollo ha tenido un gran exito en el tratamento de heridas de piel debido sus propiedades astringentes y de regeneración de los tejidos, y también por promover la proliferación de fibroblastos y la motilidad de las células epiteliales en la región del tejido lesionado. El aceite de girasol con ozono tiene la característica de penetrar em los tejidos ayudando en la homeostasis y contribuye com una acción bactericida, fungicida y aumentando el fibrinogénio de la lesión. En este trabajo se objetiva presentar um caso de la utilización del repollo y de aceite de girasol con ozono, el equino obtuve alta hospitalaria em buenas condiciones clínicas y la herida de piel em proceso final de cicatrización.

Asunto(s)

RESUMEN

This study aimed at investigating the effects of broccoli residues fermented with probiotics (BF) on the growth performance, immunity, and gut health in broilers challenged with Clostridium perfringens (C. perfringens). A total of 600 broilers (one day old) were randomly allotted into five treatments with six replicates of 20 birds each and were reared until 42 days of age. The treatments included a positive control (PC, fed a basal diet and reared on uncontaminated litter), a negative control (NC, birds reared on litter contaminated with C. perfringens and fed a basal diet), and NC plus BF at 25, 50 or 75 g/kg of diet. The BF contained yeast 3.1 × 10 (7) cfu/g, lactic acid bacteria 9.5 × 10 (6) cfu/g and Bacillus subtilis 3.5 × 10 (6) cfu/g. Birds in the NC group showed lower (p<0.05) feed intake and body weight gain, whereas BF supplementation recovered (p<0.05) the growth performance to the levels of PC group. Dietary BF at 50and 75 g/kg reduced (p<0.05) broiler mortality. Similarly, compared to the NC group, BF increased (p<0.05) immune organ weights and serum immunoglobulins A, G, and M to the levels of PC group. The ileal populations of Escherichia coli and Gram-negative bacteria were decreased (p<0.05) by BF to the levels of PC, and C. perfringens was also decreased (p<0.05) by BF. The serum profiles of mono- and di-amine oxidase were decreased (p<0.05) by BF. BF at 75 g/kg reduced (p<0.05) monoamine oxidase compared with the other BF doses. The results suggest that broccoli residues fermented with probiotics can be a novel biological feed additive to protect the performance and health of broilers against C. perfringens infection.(AU)

Asunto(s)

RESUMEN

Devido à alta frequência de lesões em membros locomotores e sua difícil cicatrização em equinos, há uma crescente busca por tratamentos alternativos e fitoterápicos, visando redução de custo e de fácil manipulação. O repolho vem sendo utilizado com êxito devido a propriedades adstringente e de regeneração tecidual, além de promover a proliferação de fibrosblastos e motilidade das células epiteliais na região do tecido lesionado. O óleo de Girassol ozonizado possui característica de penetrar nos tecidos, ocasionar homeostasia, e contribuir com ação bactericida, fungicida e aumentar fibrinogênio na lesão. Neste relato de caso, após o tratamento como Repolho e óleo de Girassol ozonizado, o equino obteve alta hospitalar em boas condições clínicas e a ferida em processo final de cicatrização.(AU)

Due to the high frequency of lesions on locomotor limbs and their difficult healing in equines, there is a growing search for alternative and phytotherapeutic treatments, aiming at cost reduction and easy manipulation. The cabbage has been used successfully due to abstergent and tissue regeneration properties, besides promoting fibroblast proliferation and epithelial cell motility in the region of the injured tissue. The ozonized sunflower oil has the characteristic of penetrating the tissues, causing homeostasis, and contributing with bactericidal, fungicidal action and increasing fibrinogen in the lesion. In this case report, after treatment with Cabbage and ozonized Sunflower oil, the horse was discharged in good clinical condition and the wound in the final healing process.(AU)

Debido la grande frecuencia de las lesiones en los membros de los equídeos y la dificultad de cicatrización de los mismo hay una búsqueda creciente por tratamentos alternativos y de fitoterapia, com el objetivo de reducir los gastos y para que sea fácil su manipulación. El repollo ha tenido un gran exito en el tratamento de heridas de piel debido sus propiedades astringentes y de regeneración de los tejidos, y también por promover la proliferación de fibroblastos y la motilidad de las células epiteliales en la región del tejido lesionado. El aceite de girasol con ozono tiene la característica de penetrar em los tejidos ayudando en la homeostasis y contribuye com una acción bactericida, fungicida y aumentando el fibrinogénio de la lesión. En este trabajo se objetiva presentar um caso de la utilización del repollo y de aceite de girasol con ozono, el equino obtuve alta hospitalaria em buenas condiciones clínicas y la herida de piel em proceso final de cicatrización.(AU)

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RESUMEN

PURPOSE: Since Technetium-99m (99mTc) has favorable physical and chemical characteristics, it is widely used radioisotope in Nuclear Medicine. However, stannous dichloride (SnCl2) has been widely used as a reducing agent in labeling procedure of pharmaceutical with radionuclide, it has been realized that SnCl2 have genotoxic and cytotoxic effects on biological systems. In previous studies, it has been shown that some herbal extract can reduce genotoxic and cytotoxic effects of SnCl2. In the present study, it is aimed to evaluate the effect of the broccoli extract on the survival of E. coli ATCC 25922 strain against to toxic effects of SnCl2. METHODS: Broccoli was extracted with methanol extraction. HPLC and TLC analysis of broccoli extract were performed. Then antitoxicity and dose response assays were performed on bacterial strain. RESULTS: The broccoli extract had dose dependent protective effect against SnCl2 toxic effect on E. coli. CONCLUSIONS: The consumption of broccoli may alter the stannous dichloride toxicity. Broccoli extract may use as a new protective strategies against the toxic effect of SnCl2 on patients who were taken 99mTc radiopharmaceuticals.(AU)

OBJETIVO: Em face de suas características físico-químicas, o Tecnécio-99m (99mTc) é um radiofármaco amplamente utilizado na Medicina Nuclear. Todavia, o dicloreto de estanho (SnCl2) tem sido largamente aplicado como um agente redutor no procedimento farmacêutico de marcação com radionuclídeos. Constatou-se que o SnCl2 apresenta efeitos genotóxicos e citotóxicos nos sistemas biológicos. Em estudos prévios, foi demonstrado que alguns extratos de ervas podem reduzir tais efeitos. O estudo atual objetivou avaliar os efeitos do extrato de brócolis na sobrevida da cepa E. coli ATCC 25922, exposta ao efeito tóxico do SnCl2. MÉTODOS: O extrato de brócolis foi obtido mediante extração com metanol. Analises com HPLC e TLC foram efetuadas. Avaliou-se a antitoxicidade e realizou-se um ensaio dose-resposta para uma cepa de bactérias. RESULTADOS: O extrato de brócolis mostrou um efeito protetor dose dependente para os efeitos tóxicos do SnCl2 sobre a E. coli. CONCLUSÕES: O consumo de brócolis pode alterar a toxicidade do dicloreto de estanho. O extrato de brócolis pode ser utilizado como uma nova estratégia para proteção de pacientes contra os efeitos tóxicos do SnCl2, nos quais foi administrado o radiofármaco Tecnécio-99m.(AU)

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